Csor.00g209060 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g209060
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionReticulon-like protein
LocationCsor_Chr05: 8412978 .. 8417155 (+)
RNA-Seq ExpressionCsor.00g209060
SyntenyCsor.00g209060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTATGGGGAGCGAGGAGAAATGGTGCGTTGTTACTGGAGGGAGAGGTTTCGCTGCACGACATCTCGTTGAGATGCTGATCAGTTCCAAAATGTTTTCCGTTCGAATCGTTGATTTAGGCTCCTCCATTGAATTGGAAGCAAGTGAGGAGAAAGGGATTCTCGGAGAGGCATTGCGTTCTGGTCGTGCTCAATATGTTTCAGCCGATCTTCGTGATAAAGCTCAATTGCTCAAAGGTTGTGATTTTTTTTCTTGAACCTTTCTGTCAACGCTATTGATTTCTGTTTTATTCGTGAAATGTGGTTGTAATGTCGATTTGAATGTTGCAGCGTTTGAAGGAGTGGAGGTTGTTTTCCATATGGCTGCTCCGAATTCATCCATTAACAATTACAAACTTCATTATTCGGTCAACGTTGAAGGTACGAACTATTTGGTTTAGCAATTCTTCTTTGATGTGCTGATTATTGTTATTGTTTTCTTTGCTTTTCAGGAGCCAAGAATGTAATTGACGCTTGTGTCGAGAAAAATGTAAAGCGGCTAATATATACTAGTTCGGCCAGTGTAGTGTTTGATGGGATAAATGGACTTGTTAATGCAGATGAGACACTGCCTTACGCATCGAAGGTACTAACTCCACTGACGACTCTGCTCATTTCATCAATAATTGGACTTTCTGTTCAGTGATTTTCGTTGCATGTTGCAAATTTTCATCGACTGTACTGCTTGGACAATGTTTGTCCACTGTTTCAGCACAATGATTTCTACTCTGCGACTAAAGCTGAGGGGGAAGCTTTGATCCTCAAGGCAAACGGCTTAAATGGACTTCTAACATGCAGCCTAAGGCCCAGTGGCATCTTTGGCCCTGGTGATAGGTTACTGGTTCCATCCCTAGTTGCTAATGCGAGGGCAGGGAAATCCAAGGTAAGCATGTTCATTATTTTTGTTAGTTTTTTTTGTTTTGTTTTGGTAAGAAACACATTTCCATCCAAAGACATGAAAGAATATACAAAGTATAAAAAACCATCCCCAACAGAATATTGAGACGATTGGGCTCTAACTACAGAAAAGAACTCAAATCCCAAAGAATAAGAGCAAGCTCCCAATGACGAAAGGACCTAGTGACACTCACAAAGAGGCGCTAATCCTATCCGCTTCCTGACTTCCTGACCAATCTCTCAATCTTCTAGAGATTCTATTTAAGTTCCATTTTAGTTTTTTTTCACTTTGTCAAAAATTTTCTGACGTATAATTTGGAACTTGGAGTCTTGGAGCTACTTCTGTCATCAGGCTCTTGTTTAGATACCAAAATATTATATTTATGTTTTTATAAGGCTTTCAGCTTGCCCCTAATTTTAATCCAGAAGTTGGCAGTGGACAAGCAATCAAAATCCAAGATCTCCATTTCCCACTGAAGCCCTTAACAGAATGATATCCCAAAATGACCAATGTACTTTGTCGCATACCTTTTCAATTTCAAGTTCAAGCACTAGCCTTTTCATGGTCGTTTTGCAGTCATCAATTAGCTGGTTGGCAAGAATAATGGAACCAACCATTTGTCTTTCCTCTTTATAGAGGCCAATAACTAATAAGCCTTAATTAAATGATGTAAATCTGTTATTGATTTAGCTATAAAAAAGACTATTCTTTCGTTTTAGTTTTATACCTCCTGCTACCATCTAAGATTAGACTGATTTGAGATTAGATTGATGTTGCTCTCTCTTATCCTAGAGTTTACAACTTCATCTGGAAAGTCGTAGAACACCTTGATAAAATCAACCTGGATGACATTTGAAGTCCTTGGAGCGTTGTTGGATCATAATTTTTTTTGTCATTCCATACCGGAGAGGAAGCTATGACTGCCTACTGGAAAGGAACCCCCCGGTAGAATTTAAAAACTATCCTAAAAAACTTGAAAACTTTTGATACAGGTTAATGGTCTCCCTTCCACATCCAAATAATTGGTCATTAGAAATCTCTTGGTAAATGAAACTCTTCTTCTTTTGCTTTTTAACTTGAAGGTGGAATCAGAAAACAGAATCTTCAACACTGTTTTAAATGTTCATCTTGTTATTGCATCTTTGCCTTCACATAGTGTCCACCTTCTTCCCTTTCAAATGACACTTTCTGCACTCTCTTTGGTACACACCCAAGCTGCTCAATAGAAAGACCAACCACCTGTTCTCCATGCCATCCAAAAAATGAAGCTCGGAGAAAATGGCATCCTTTTGAGCTGCAATATTACCATGTATTACACAGGGATTAATTGAAATTATTTTTATTTTCATTTGTCTTATAGCCTAATTTATAATAGCATTTGATCTAATCCCCTCTTCAAGATTATAGGATGAATTTGATTTGCTGTTCCTCTCTTCTCAAAATCCTATGTACAAATACATATATATGTTATTAATATTATTTTGAACAAGAAACAACTTTTATCAATAAATGAAAAGGAAGATAAGTGTTAAAGGATACAAGTTCCAAAGAGAACGAAGAAAATAAAAAACAGGCCTACAAAATATGTATGGGAAGTCTATACAGTTTTCTTTCTATCGCCCTATGCAATTACATTTCATAAAAAATAAAAATAAAAAAAAGTCATGGCCTTCATTGTAATTGTCATGTGTTTTAAAATTTACAGTTCATTATTGGTAAGGGCAACAACACCTATGACTTTACTTATGTTGAAAATGTGGGACATGCCCATGTGTGTGCTGAGCGAGCTCTAGCTTCAGGAGGAATGATTGCAGAAAAAGCTGCAGGGCAGGTACCTCTGTCAGTGTATGGAACGACTTAATTATATTCAGTTATCCTGGTCTTCTCTGTCTAATGAATAATCGAATTGTAGTATTGCATGATATATATAAGTTTTCCTACCGGACAAAGAATTTATAGAAGTTGGGTTCTTGTTTCTTTTTTTTTTTTTAAATGTAATGGATGATAAAACAAAAATCATAGTTTCGTCACATTTAGTTCTTGGTTATAAACCATTGGAATATTAATGGTATGCTTGTCTCTTGTCTTCGATACTCTCATTACAATAATTTTCATTGTGCATATAGCACCAGTCTAGCAGCAAATTAACATCTTTGACATATGTATTCAGTACTTGTCCGTGAGCATAGGAGAAATTGTGATGTTAAAGTATTTACTGCAGATCAATTAATAAAAAAGCAATTTAATGGATTAAACTCAGGACTCTCAAGGAAGCTTAATTGTTGTCAAATTTGTTGCTAGTAAAGTTGAACATTAGGGTTCGGTTAATTTCGAACTTAAATGTAAAATAGTAAAATAAAAGAGAAACTTCTAGAAGTTGGTTAGAAGTTATAAGAACTTTTAGGGAGTCAATCTCAAAAGCAATAGTTGACCTGGTCAACGTAATTGAAAAAGTGATTCTATTTAAATTAATTTATAATCAGTTTTATCAAACACTCGAAACAGTTAAATACCCCCATAGTCACATTTAAGGCCGGAAGAATCAATCTCAAAGGGAGCTATAGTCTTAAATTAATTTATAATCAATTTTACCGAACACTAAACACAATTAGAATTTCTCCATAATCACTTTCAAGACCTGCAAAATCAATTTTAAACAGAGCCTTAGACTAAACTTGAAGGTCTTTTGTAGGCTTATTTCATAACCAACATGGAACCCATAAAGTTTTGGGAGTTTGTCTCACTTATACTGGATGGCCTTGGCTATGAAAGGTATTCTTTATTGTAATTTAAATTTTAGCCCCTCTGATCATTTGGTTTTCCAGCCATCTTTATCCTCGTCTTGGTAAAGCTAAGATATGCTGTATTGACATCTTGCATTCTCTGAGTTGCAGGCCAAGAATAAAAATCCCTGCCTACCTAGTGATGCCGATTGCACATATGGTGAACTGGATTTATACGCTTTTAGGTCCTTATGGAATGCCTGTCCCACAGTTTACTCCTTCCCGTATAAGACTCCTCTCTTGTTGTAGAACTTTTAACTCCTCAAAGGCCAAGGATCGACTTTTCTATACCCCCATTGTTTCACTTCAGGTTCTTCTATGTTTTATTTGTTGCATATACTTTTTTCTTGTGCATGTTAATGCTATTTTTTAACTCTCTACTATACTCTTTAAATGCCTTCCTTTGATGATGTTTCTGTAGGAACTAAAGAAAACCATGTGTATTTTGCTCTACTGA

mRNA sequence

ATGAGTATGGGGAGCGAGGAGAAATGGTGCGTTGTTACTGGAGGGAGAGGTTTCGCTGCACGACATCTCGTTGAGATGCTGATCAGTTCCAAAATGTTTTCCGTTCGAATCGTTGATTTAGGCTCCTCCATTGAATTGGAAGCAAGTGAGGAGAAAGGGATTCTCGGAGAGGCATTGCGTTCTGGTCGTGCTCAATATGTTTCAGCCGATCTTCGTGATAAAGCTCAATTGCTCAAAGCGTTTGAAGGAGTGGAGGTTGTTTTCCATATGGCTGCTCCGAATTCATCCATTAACAATTACAAACTTCATTATTCGGTCAACGTTGAAGGAGCCAAGAATGTAATTGACGCTTGTGTCGAGAAAAATGTAAAGCGGCTAATATATACTAGTTCGGCCAGTGTAGTGTTTGATGGGATAAATGGACTTGTTAATGCAGATGAGACACTGCCTTACGCATCGAAGCACAATGATTTCTACTCTGCGACTAAAGCTGAGGGGGAAGCTTTGATCCTCAAGGCAAACGGCTTAAATGGACTTCTAACATGCAGCCTAAGGCCCAGTGGCATCTTTGGCCCTGGTGATAGGTTACTGGTTCCATCCCTAGTTGCTAATGCGAGGGCAGGGAAATCCAAGTTCATTATTGGTAAGGGCAACAACACCTATGACTTTACTTATGTTGAAAATGTGGGACATGCCCATGTGTGTGCTGAGCGAGCTCTAGCTTCAGGAGGAATGATTGCAGAAAAAGCTGCAGGGCAGGCTTATTTCATAACCAACATGGAACCCATAAAGTTTTGGGAGTTTGTCTCACTTATACTGGATGGCCTTGGCTATGAAAGGCCAAGAATAAAAATCCCTGCCTACCTAGTGATGCCGATTGCACATATGGTGAACTGGATTTATACGCTTTTAGGTCCTTATGGAATGCCTGTCCCACAGTTTACTCCTTCCCGTATAAGACTCCTCTCTTGTTGTAGAACTTTTAACTCCTCAAAGGCCAAGGATCGACTTTTCTATACCCCCATTGTTTCACTTCAGGAACTAAAGAAAACCATGTGTATTTTGCTCTACTGA

Coding sequence (CDS)

ATGAGTATGGGGAGCGAGGAGAAATGGTGCGTTGTTACTGGAGGGAGAGGTTTCGCTGCACGACATCTCGTTGAGATGCTGATCAGTTCCAAAATGTTTTCCGTTCGAATCGTTGATTTAGGCTCCTCCATTGAATTGGAAGCAAGTGAGGAGAAAGGGATTCTCGGAGAGGCATTGCGTTCTGGTCGTGCTCAATATGTTTCAGCCGATCTTCGTGATAAAGCTCAATTGCTCAAAGCGTTTGAAGGAGTGGAGGTTGTTTTCCATATGGCTGCTCCGAATTCATCCATTAACAATTACAAACTTCATTATTCGGTCAACGTTGAAGGAGCCAAGAATGTAATTGACGCTTGTGTCGAGAAAAATGTAAAGCGGCTAATATATACTAGTTCGGCCAGTGTAGTGTTTGATGGGATAAATGGACTTGTTAATGCAGATGAGACACTGCCTTACGCATCGAAGCACAATGATTTCTACTCTGCGACTAAAGCTGAGGGGGAAGCTTTGATCCTCAAGGCAAACGGCTTAAATGGACTTCTAACATGCAGCCTAAGGCCCAGTGGCATCTTTGGCCCTGGTGATAGGTTACTGGTTCCATCCCTAGTTGCTAATGCGAGGGCAGGGAAATCCAAGTTCATTATTGGTAAGGGCAACAACACCTATGACTTTACTTATGTTGAAAATGTGGGACATGCCCATGTGTGTGCTGAGCGAGCTCTAGCTTCAGGAGGAATGATTGCAGAAAAAGCTGCAGGGCAGGCTTATTTCATAACCAACATGGAACCCATAAAGTTTTGGGAGTTTGTCTCACTTATACTGGATGGCCTTGGCTATGAAAGGCCAAGAATAAAAATCCCTGCCTACCTAGTGATGCCGATTGCACATATGGTGAACTGGATTTATACGCTTTTAGGTCCTTATGGAATGCCTGTCCCACAGTTTACTCCTTCCCGTATAAGACTCCTCTCTTGTTGTAGAACTTTTAACTCCTCAAAGGCCAAGGATCGACTTTTCTATACCCCCATTGTTTCACTTCAGGAACTAAAGAAAACCATGTGTATTTTGCTCTACTGA

Protein sequence

MSMGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEKGILGEALRSGRAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVEKNVKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLLTCSLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERALASGGMIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWIYTLLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQELKKTMCILLY
Homology
BLAST of Csor.00g209060 vs. ExPASy Swiss-Prot
Match: Q67ZE1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 OS=Arabidopsis thaliana OX=3702 GN=3BETAHSD/D2 PE=1 SV=2)

HSP 1 Score: 528.5 bits (1360), Expect = 5.9e-149
Identity = 255/341 (74.78%), Postives = 293/341 (85.92%), Query Frame = 0

Query: 7   EKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEKGILGEALRSGRAQY 66
           E+WCVVTGGRGFAARHLVEML+  +MF VRI DL  +I L+  E  G+L E LRSGR QY
Sbjct: 9   ERWCVVTGGRGFAARHLVEMLVRYEMFCVRIADLAPAIMLDPQEGNGVLDEGLRSGRVQY 68

Query: 67  VSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVEKNVKRL 126
           +SADLRDK+Q++KAF+G EVVFHMAAP+SSINN++L YSVNV+G +NVIDACV+  VKRL
Sbjct: 69  ISADLRDKSQVVKAFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTQNVIDACVDVGVKRL 128

Query: 127 IYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLLTCSLRP 186
           IYTSS SVVFDG++G++N  E++ Y  KHND YSATKAEGE LI+KANG NGLLTC +RP
Sbjct: 129 IYTSSPSVVFDGVHGILNGTESMAYPIKHNDSYSATKAEGEELIMKANGRNGLLTCCIRP 188

Query: 187 SGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERALASGGMI 246
           S IFGPGDRLLVPSLVA ARAGKSKFIIG GNN YDFTYVENV HAHVCAERALASGG +
Sbjct: 189 SSIFGPGDRLLVPSLVAAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAERALASGGDV 248

Query: 247 AEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWIYTLLGP 306
           + KAAGQAYFITNMEPIKFWEF+S +LDGLGYERP IKIPA+++MPIAH+V   Y +LGP
Sbjct: 249 STKAAGQAYFITNMEPIKFWEFMSQLLDGLGYERPSIKIPAFIMMPIAHLVELTYKVLGP 308

Query: 307 YGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQE 348
           YGM VPQ TPSR+RLLSC RTF+S+KAKDRL Y P+V LQE
Sbjct: 309 YGMTVPQLTPSRVRLLSCSRTFDSTKAKDRLGYAPVVPLQE 349

BLAST of Csor.00g209060 vs. ExPASy Swiss-Prot
Match: Q9FX01 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 OS=Arabidopsis thaliana OX=3702 GN=3BETAHSD/D1 PE=1 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 1.3e-148
Identity = 261/353 (73.94%), Postives = 299/353 (84.70%), Query Frame = 0

Query: 1   MSMGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEKGILGEALR 60
           M +   E+WCVVTGGRGFAARHLVEML+  +MF VRI DL  +I L   EE GILGEA+R
Sbjct: 3   MEVTETERWCVVTGGRGFAARHLVEMLVRYQMFHVRIADLAPAIVLNPHEETGILGEAIR 62

Query: 61  SGRAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVE 120
           SGR QYVSADLR+K Q++K F+G EVVFHMAAP+SSINN++L YSVNV+G  NVIDAC+E
Sbjct: 63  SGRVQYVSADLRNKTQVVKGFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTTNVIDACIE 122

Query: 121 KNVKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLL 180
             VKRLIYTSS SVVFDG++G +NADE+LPY  KHND YSATKAEGEALILKANG +GLL
Sbjct: 123 VGVKRLIYTSSPSVVFDGVHGTLNADESLPYPPKHNDSYSATKAEGEALILKANGRSGLL 182

Query: 181 TCSLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERAL 240
           TC +RPS IFGPGD+L+VPSLV  ARAGKSKFIIG G+N YDFTYVENV HAHVCAERAL
Sbjct: 183 TCCIRPSSIFGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTYVENVVHAHVCAERAL 242

Query: 241 ASGGMIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWI 300
           ASGG +  KAAGQAYFITNMEPIKFWEF+S +L+GLGYERP IKIPA L+MPIA++V   
Sbjct: 243 ASGGEVCAKAAGQAYFITNMEPIKFWEFMSQLLEGLGYERPSIKIPASLMMPIAYLVELA 302

Query: 301 YTLLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQE-LKKTM 353
           Y LLGPYGM VP  TPSR+RLLSC RTF+SSKAKDRL Y+P+V LQE +K+T+
Sbjct: 303 YKLLGPYGMKVPVLTPSRVRLLSCNRTFDSSKAKDRLGYSPVVPLQEGIKRTI 355

BLAST of Csor.00g209060 vs. ExPASy Swiss-Prot
Match: A9X4U2 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 3 OS=Arabidopsis thaliana OX=3702 GN=3BETAHSD/D3 PE=2 SV=2)

HSP 1 Score: 283.1 bits (723), Expect = 4.3e-75
Identity = 158/344 (45.93%), Postives = 216/344 (62.79%), Query Frame = 0

Query: 5   SEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEKGILGEALRSGRA 64
           S  K CVV GGRGF  R LV  L+    ++VR+ D G ++ L+ S+   +L +AL SGRA
Sbjct: 10  SHLKTCVVLGGRGFIGRSLVSRLLRLGNWTVRVADSGHTLHLDESD--SLLEDALSSGRA 69

Query: 65  QYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVEKNVK 124
            Y   D+RDK Q++K  EG  VVF+M A +   ++Y   Y V V+G +NVI AC E  V+
Sbjct: 70  SYHCVDVRDKPQIVKVTEGSYVVFYMGATDLRSHDYFDCYKVIVQGTRNVISACRESGVR 129

Query: 125 RLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLLTCSL 184
           +LIY S+A VVFDG   + + DE+L    K     +  KA+ EALI  AN  +GLLTC+L
Sbjct: 130 KLIYNSTADVVFDGSQPIRDGDESLRRPLKFQSMLTDFKAQAEALIKLANNRDGLLTCAL 189

Query: 185 RPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERALASGG 244
           R S +FGPGD   VP LV  A++G +KFI+G G N  DFTY ENV HAH+CA +AL S  
Sbjct: 190 RSSIVFGPGDTEFVPFLVNLAKSGYAKFILGSGENISDFTYSENVSHAHICAVKALDSQ- 249

Query: 245 MIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWIYTLL 304
              E  AG+ +FITN++P++FW+FVS I++GLGY RP IK+P  LV+ +  ++ W +   
Sbjct: 250 --MEFVAGKEFFITNLKPVRFWDFVSHIVEGLGYPRPSIKLPVRLVLYVFSLLKWTHE-- 309

Query: 305 GPYGMPVPQFTPSRIRLL-SCCRTFNSSKAKDRLFYTPIVSLQE 348
              G+     T  +  LL S  RTFN + AK  L YTP+V+L++
Sbjct: 310 -KEGLGSNYDTAHQYALLASSTRTFNCNAAKKHLGYTPVVTLED 345

BLAST of Csor.00g209060 vs. ExPASy Swiss-Prot
Match: Q9R1J0 (Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Mus musculus OX=10090 GN=Nsdhl PE=1 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 3.2e-62
Identity = 137/344 (39.83%), Postives = 191/344 (55.52%), Query Frame = 0

Query: 5   SEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEKGILGEALRSGRA 64
           S+ K C V GG GF  +H+VE L+  + ++V + D+    +               + R 
Sbjct: 24  SKAKKCTVIGGSGFLGQHMVEQLL-ERGYTVNVFDIHQGFD---------------NPRV 83

Query: 65  QYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVEKNVK 124
           Q+   DL ++  L  A +GV  VFH A+P    NN +L Y VN  G K VI+ C E  V+
Sbjct: 84  QFFIGDLCNQQDLYPALKGVSTVFHCASPPPYSNNKELFYRVNFIGTKTVIETCREAGVQ 143

Query: 125 RLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANG-LNGLLTCS 184
           +LI TSSASVVF+G++ + N  E LPYA K  D+Y+ TK   E  +L AN      LT +
Sbjct: 144 KLILTSSASVVFEGVD-IKNGTEDLPYAMKPIDYYTETKILQERAVLDANDPKKNFLTAA 203

Query: 185 LRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERALASG 244
           +RP GIFGP D  LVP L+  AR GK KF+IG G N  DFT+VENV H H+ A   L+  
Sbjct: 204 IRPHGIFGPRDPQLVPILIDAARKGKMKFMIGNGENLVDFTFVENVVHGHILAAEHLSQD 263

Query: 245 GMIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWIYTL 304
             +     G+A+ ITN EPI FW F+S IL GL YE P+  IP ++   +A +++ +  +
Sbjct: 264 AAL----GGKAFHITNDEPIPFWTFLSRILTGLNYEAPKYHIPYWMAYYLAFLLSLLVMV 323

Query: 305 LGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQE 348
           + P     P FTP R+ L      ++  KAK    Y P+V++ E
Sbjct: 324 VSPLIQIQPTFTPIRVALAGTFHYYSCEKAKKLFGYRPLVTMDE 346

BLAST of Csor.00g209060 vs. ExPASy Swiss-Prot
Match: Q5PPL3 (Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Rattus norvegicus OX=10116 GN=Nsdhl PE=2 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 4.2e-62
Identity = 138/344 (40.12%), Postives = 193/344 (56.10%), Query Frame = 0

Query: 5   SEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEKGILGEALRSGRA 64
           S+ K C V GG GF  +H+VE L+ S+ ++V + D+    +               + R 
Sbjct: 24  SKAKKCTVIGGSGFLGQHMVEQLL-SRGYAVNVFDVRQGFD---------------NPRV 83

Query: 65  QYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVEKNVK 124
           Q+   DL ++  L  A +GV  VFH A+P S+ NN +L Y VN  G K VI+ C E  V+
Sbjct: 84  QFFIGDLCNQQDLYPALKGVSTVFHCASPPSNSNNKELFYRVNSTGTKTVIETCKEAGVQ 143

Query: 125 RLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANG-LNGLLTCS 184
           +LI TSSASVVF+G++ + N  E LPYA K  D+Y+ TK   E  +L AN      LT +
Sbjct: 144 KLILTSSASVVFEGVD-IKNGTEDLPYAMKPIDYYTETKILQERAVLDANDPKKNFLTAA 203

Query: 185 LRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERALASG 244
           +RP GIFGP D  LVP L+  AR GK KF+IG G N  DFT+VENV H H+ A   L+  
Sbjct: 204 IRPHGIFGPRDPQLVPVLIDAARKGKMKFMIGNGKNLVDFTFVENVVHGHILAAEHLSRD 263

Query: 245 GMIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWIYTL 304
             +     G+A+ ITN EPI FW F+S IL GL YE P+  IP  +   +A +++ +  +
Sbjct: 264 AGL----GGKAFHITNDEPIPFWTFLSRILTGLNYEAPKYHIPYRVAYYLAFLLSLLVMV 323

Query: 305 LGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQE 348
           L P       FTP R+ L      ++  KAK  + Y P+V++ +
Sbjct: 324 LSPLIQIQTTFTPFRVALAGTFHYYSCEKAKKLIGYRPLVTMDD 346

BLAST of Csor.00g209060 vs. NCBI nr
Match: KAG6599124.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 712 bits (1839), Expect = 5.63e-259
Identity = 357/357 (100.00%), Postives = 357/357 (100.00%), Query Frame = 0

Query: 1   MSMGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEKGILGEALR 60
           MSMGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEKGILGEALR
Sbjct: 1   MSMGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEKGILGEALR 60

Query: 61  SGRAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVE 120
           SGRAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVE
Sbjct: 61  SGRAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVE 120

Query: 121 KNVKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLL 180
           KNVKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLL
Sbjct: 121 KNVKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLL 180

Query: 181 TCSLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERAL 240
           TCSLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERAL
Sbjct: 181 TCSLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERAL 240

Query: 241 ASGGMIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWI 300
           ASGGMIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWI
Sbjct: 241 ASGGMIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWI 300

Query: 301 YTLLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQELKKTMCILLY 357
           YTLLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQELKKTMCILLY
Sbjct: 301 YTLLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQELKKTMCILLY 357

BLAST of Csor.00g209060 vs. NCBI nr
Match: XP_022946821.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita moschata] >XP_022946822.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita moschata] >XP_022946824.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita moschata] >XP_022946825.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita moschata])

HSP 1 Score: 693 bits (1788), Expect = 5.69e-248
Identity = 346/347 (99.71%), Postives = 347/347 (100.00%), Query Frame = 0

Query: 1   MSMGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEKGILGEALR 60
           MSMGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEKGILGEALR
Sbjct: 1   MSMGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEKGILGEALR 60

Query: 61  SGRAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVE 120
           SGRAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGA+NVIDACVE
Sbjct: 61  SGRAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGARNVIDACVE 120

Query: 121 KNVKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLL 180
           KNVKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLL
Sbjct: 121 KNVKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLL 180

Query: 181 TCSLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERAL 240
           TCSLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERAL
Sbjct: 181 TCSLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERAL 240

Query: 241 ASGGMIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWI 300
           ASGGMIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWI
Sbjct: 241 ASGGMIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWI 300

Query: 301 YTLLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQE 347
           YTLLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQE
Sbjct: 301 YTLLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQE 347

BLAST of Csor.00g209060 vs. NCBI nr
Match: XP_023546590.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita pepo subsp. pepo] >XP_023546591.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita pepo subsp. pepo] >XP_023546592.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita pepo subsp. pepo] >XP_023546593.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 691 bits (1783), Expect = 3.28e-247
Identity = 345/347 (99.42%), Postives = 347/347 (100.00%), Query Frame = 0

Query: 1   MSMGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEKGILGEALR 60
           MSMGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEE+GILGEALR
Sbjct: 1   MSMGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEQGILGEALR 60

Query: 61  SGRAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVE 120
           SGRAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVE
Sbjct: 61  SGRAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVE 120

Query: 121 KNVKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLL 180
           KNVKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLL
Sbjct: 121 KNVKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLL 180

Query: 181 TCSLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERAL 240
           TCSLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERAL
Sbjct: 181 TCSLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERAL 240

Query: 241 ASGGMIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWI 300
           ASGG+IAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWI
Sbjct: 241 ASGGIIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWI 300

Query: 301 YTLLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQE 347
           YTLLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQE
Sbjct: 301 YTLLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQE 347

BLAST of Csor.00g209060 vs. NCBI nr
Match: XP_022999664.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita maxima] >XP_022999665.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita maxima] >XP_022999667.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita maxima] >XP_022999668.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita maxima])

HSP 1 Score: 687 bits (1774), Expect = 7.67e-246
Identity = 344/347 (99.14%), Postives = 346/347 (99.71%), Query Frame = 0

Query: 1   MSMGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEKGILGEALR 60
           MSMGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEE+GILGEALR
Sbjct: 1   MSMGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEQGILGEALR 60

Query: 61  SGRAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVE 120
           SGRAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVE
Sbjct: 61  SGRAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVE 120

Query: 121 KNVKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLL 180
           KNVKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLL
Sbjct: 121 KNVKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLL 180

Query: 181 TCSLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERAL 240
           TCSLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERAL
Sbjct: 181 TCSLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERAL 240

Query: 241 ASGGMIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWI 300
           ASGG+IAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPA LVMPIAHMVNWI
Sbjct: 241 ASGGIIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPANLVMPIAHMVNWI 300

Query: 301 YTLLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQE 347
           YTLLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQE
Sbjct: 301 YTLLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQE 347

BLAST of Csor.00g209060 vs. NCBI nr
Match: KAA0064699.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform X1 [Cucumis melo var. makuwa] >TYK00715.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 651 bits (1679), Expect = 2.68e-234
Identity = 321/349 (91.98%), Postives = 334/349 (95.70%), Query Frame = 0

Query: 3   MGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEKGILGEALRSG 62
           MGSEEKWCVVTGGRGFAARHLVEMLI   MFSVRIVDLGSSIELEASEE+G LGEALRSG
Sbjct: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60

Query: 63  RAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVEKN 122
           RAQYVSADLRDKAQ+LKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVID+CVE+N
Sbjct: 61  RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120

Query: 123 VKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLLTC 182
           VKRLIYTSSASVVFDGIN L+N DE++PYASKHNDFYSATKAEGEAL+LKANG NGLLTC
Sbjct: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANGTNGLLTC 180

Query: 183 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERALAS 242
           SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIG GNNTYDFTYVENVGHAHVCAERALA+
Sbjct: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240

Query: 243 GGMIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWIYT 302
           GG+I+E+AAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPA +VMPIAHMVNW Y 
Sbjct: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300

Query: 303 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQELKKT 351
           LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQ    T
Sbjct: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQVFLST 349

BLAST of Csor.00g209060 vs. ExPASy TrEMBL
Match: A0A6J1G542 (Reticulon-like protein OS=Cucurbita moschata OX=3662 GN=LOC111450770 PE=4 SV=1)

HSP 1 Score: 693 bits (1788), Expect = 2.75e-248
Identity = 346/347 (99.71%), Postives = 347/347 (100.00%), Query Frame = 0

Query: 1   MSMGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEKGILGEALR 60
           MSMGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEKGILGEALR
Sbjct: 1   MSMGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEKGILGEALR 60

Query: 61  SGRAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVE 120
           SGRAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGA+NVIDACVE
Sbjct: 61  SGRAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGARNVIDACVE 120

Query: 121 KNVKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLL 180
           KNVKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLL
Sbjct: 121 KNVKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLL 180

Query: 181 TCSLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERAL 240
           TCSLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERAL
Sbjct: 181 TCSLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERAL 240

Query: 241 ASGGMIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWI 300
           ASGGMIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWI
Sbjct: 241 ASGGMIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWI 300

Query: 301 YTLLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQE 347
           YTLLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQE
Sbjct: 301 YTLLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQE 347

BLAST of Csor.00g209060 vs. ExPASy TrEMBL
Match: A0A6J1KG57 (Reticulon-like protein OS=Cucurbita maxima OX=3661 GN=LOC111493953 PE=4 SV=1)

HSP 1 Score: 687 bits (1774), Expect = 3.71e-246
Identity = 344/347 (99.14%), Postives = 346/347 (99.71%), Query Frame = 0

Query: 1   MSMGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEKGILGEALR 60
           MSMGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEE+GILGEALR
Sbjct: 1   MSMGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEQGILGEALR 60

Query: 61  SGRAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVE 120
           SGRAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVE
Sbjct: 61  SGRAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVE 120

Query: 121 KNVKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLL 180
           KNVKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLL
Sbjct: 121 KNVKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLL 180

Query: 181 TCSLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERAL 240
           TCSLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERAL
Sbjct: 181 TCSLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERAL 240

Query: 241 ASGGMIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWI 300
           ASGG+IAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPA LVMPIAHMVNWI
Sbjct: 241 ASGGIIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPANLVMPIAHMVNWI 300

Query: 301 YTLLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQE 347
           YTLLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQE
Sbjct: 301 YTLLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQE 347

BLAST of Csor.00g209060 vs. ExPASy TrEMBL
Match: A0A5D3BNL7 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold187G00020 PE=3 SV=1)

HSP 1 Score: 651 bits (1679), Expect = 1.30e-234
Identity = 321/349 (91.98%), Postives = 334/349 (95.70%), Query Frame = 0

Query: 3   MGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEKGILGEALRSG 62
           MGSEEKWCVVTGGRGFAARHLVEMLI   MFSVRIVDLGSSIELEASEE+G LGEALRSG
Sbjct: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60

Query: 63  RAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVEKN 122
           RAQYVSADLRDKAQ+LKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVID+CVE+N
Sbjct: 61  RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120

Query: 123 VKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLLTC 182
           VKRLIYTSSASVVFDGIN L+N DE++PYASKHNDFYSATKAEGEAL+LKANG NGLLTC
Sbjct: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANGTNGLLTC 180

Query: 183 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERALAS 242
           SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIG GNNTYDFTYVENVGHAHVCAERALA+
Sbjct: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240

Query: 243 GGMIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWIYT 302
           GG+I+E+AAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPA +VMPIAHMVNW Y 
Sbjct: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300

Query: 303 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQELKKT 351
           LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQ    T
Sbjct: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQVFLST 349

BLAST of Csor.00g209060 vs. ExPASy TrEMBL
Match: A0A1S3BDV0 (Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103488530 PE=4 SV=1)

HSP 1 Score: 653 bits (1684), Expect = 6.95e-233
Identity = 321/345 (93.04%), Postives = 334/345 (96.81%), Query Frame = 0

Query: 3   MGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEKGILGEALRSG 62
           MGSEEKWCVVTGGRGFAARHLVEMLI   MFSVRIVDLGSSIELEASEE+G LGEALRSG
Sbjct: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60

Query: 63  RAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVEKN 122
           RAQYVSADLRDKAQ+LKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVID+CVE+N
Sbjct: 61  RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120

Query: 123 VKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLLTC 182
           VKRLIYTSSASVVFDGIN L+N DE++PYASKHNDFYSATKAEGEAL+LKANG NGLLTC
Sbjct: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANGTNGLLTC 180

Query: 183 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERALAS 242
           SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIG GNNTYDFTYVENVGHAHVCAERALA+
Sbjct: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240

Query: 243 GGMIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWIYT 302
           GG+I+E+AAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPA +VMPIAHMVNW Y 
Sbjct: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300

Query: 303 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQE 347
           LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQE
Sbjct: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQE 345

BLAST of Csor.00g209060 vs. ExPASy TrEMBL
Match: A0A1S3BDP7 (Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103488530 PE=4 SV=1)

HSP 1 Score: 653 bits (1684), Expect = 1.69e-232
Identity = 321/345 (93.04%), Postives = 334/345 (96.81%), Query Frame = 0

Query: 3   MGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEKGILGEALRSG 62
           MGSEEKWCVVTGGRGFAARHLVEMLI   MFSVRIVDLGSSIELEASEE+G LGEALRSG
Sbjct: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60

Query: 63  RAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVEKN 122
           RAQYVSADLRDKAQ+LKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVID+CVE+N
Sbjct: 61  RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120

Query: 123 VKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLLTC 182
           VKRLIYTSSASVVFDGIN L+N DE++PYASKHNDFYSATKAEGEAL+LKANG NGLLTC
Sbjct: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANGTNGLLTC 180

Query: 183 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERALAS 242
           SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIG GNNTYDFTYVENVGHAHVCAERALA+
Sbjct: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240

Query: 243 GGMIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWIYT 302
           GG+I+E+AAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPA +VMPIAHMVNW Y 
Sbjct: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300

Query: 303 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQE 347
           LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQE
Sbjct: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQE 345

BLAST of Csor.00g209060 vs. TAIR 10
Match: AT2G26260.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 )

HSP 1 Score: 528.5 bits (1360), Expect = 4.2e-150
Identity = 255/341 (74.78%), Postives = 293/341 (85.92%), Query Frame = 0

Query: 7   EKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEKGILGEALRSGRAQY 66
           E+WCVVTGGRGFAARHLVEML+  +MF VRI DL  +I L+  E  G+L E LRSGR QY
Sbjct: 9   ERWCVVTGGRGFAARHLVEMLVRYEMFCVRIADLAPAIMLDPQEGNGVLDEGLRSGRVQY 68

Query: 67  VSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVEKNVKRL 126
           +SADLRDK+Q++KAF+G EVVFHMAAP+SSINN++L YSVNV+G +NVIDACV+  VKRL
Sbjct: 69  ISADLRDKSQVVKAFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTQNVIDACVDVGVKRL 128

Query: 127 IYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLLTCSLRP 186
           IYTSS SVVFDG++G++N  E++ Y  KHND YSATKAEGE LI+KANG NGLLTC +RP
Sbjct: 129 IYTSSPSVVFDGVHGILNGTESMAYPIKHNDSYSATKAEGEELIMKANGRNGLLTCCIRP 188

Query: 187 SGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERALASGGMI 246
           S IFGPGDRLLVPSLVA ARAGKSKFIIG GNN YDFTYVENV HAHVCAERALASGG +
Sbjct: 189 SSIFGPGDRLLVPSLVAAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAERALASGGDV 248

Query: 247 AEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWIYTLLGP 306
           + KAAGQAYFITNMEPIKFWEF+S +LDGLGYERP IKIPA+++MPIAH+V   Y +LGP
Sbjct: 249 STKAAGQAYFITNMEPIKFWEFMSQLLDGLGYERPSIKIPAFIMMPIAHLVELTYKVLGP 308

Query: 307 YGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQE 348
           YGM VPQ TPSR+RLLSC RTF+S+KAKDRL Y P+V LQE
Sbjct: 309 YGMTVPQLTPSRVRLLSCSRTFDSTKAKDRLGYAPVVPLQE 349

BLAST of Csor.00g209060 vs. TAIR 10
Match: AT1G47290.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 )

HSP 1 Score: 527.3 bits (1357), Expect = 9.4e-150
Identity = 261/353 (73.94%), Postives = 299/353 (84.70%), Query Frame = 0

Query: 1   MSMGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEKGILGEALR 60
           M +   E+WCVVTGGRGFAARHLVEML+  +MF VRI DL  +I L   EE GILGEA+R
Sbjct: 3   MEVTETERWCVVTGGRGFAARHLVEMLVRYQMFHVRIADLAPAIVLNPHEETGILGEAIR 62

Query: 61  SGRAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVE 120
           SGR QYVSADLR+K Q++K F+G EVVFHMAAP+SSINN++L YSVNV+G  NVIDAC+E
Sbjct: 63  SGRVQYVSADLRNKTQVVKGFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTTNVIDACIE 122

Query: 121 KNVKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLL 180
             VKRLIYTSS SVVFDG++G +NADE+LPY  KHND YSATKAEGEALILKANG +GLL
Sbjct: 123 VGVKRLIYTSSPSVVFDGVHGTLNADESLPYPPKHNDSYSATKAEGEALILKANGRSGLL 182

Query: 181 TCSLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERAL 240
           TC +RPS IFGPGD+L+VPSLV  ARAGKSKFIIG G+N YDFTYVENV HAHVCAERAL
Sbjct: 183 TCCIRPSSIFGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTYVENVVHAHVCAERAL 242

Query: 241 ASGGMIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWI 300
           ASGG +  KAAGQAYFITNMEPIKFWEF+S +L+GLGYERP IKIPA L+MPIA++V   
Sbjct: 243 ASGGEVCAKAAGQAYFITNMEPIKFWEFMSQLLEGLGYERPSIKIPASLMMPIAYLVELA 302

Query: 301 YTLLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQE-LKKTM 353
           Y LLGPYGM VP  TPSR+RLLSC RTF+SSKAKDRL Y+P+V LQE +K+T+
Sbjct: 303 YKLLGPYGMKVPVLTPSRVRLLSCNRTFDSSKAKDRLGYSPVVPLQEGIKRTI 355

BLAST of Csor.00g209060 vs. TAIR 10
Match: AT1G47290.2 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 )

HSP 1 Score: 527.3 bits (1357), Expect = 9.4e-150
Identity = 261/353 (73.94%), Postives = 299/353 (84.70%), Query Frame = 0

Query: 1   MSMGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEKGILGEALR 60
           M +   E+WCVVTGGRGFAARHLVEML+  +MF VRI DL  +I L   EE GILGEA+R
Sbjct: 3   MEVTETERWCVVTGGRGFAARHLVEMLVRYQMFHVRIADLAPAIVLNPHEETGILGEAIR 62

Query: 61  SGRAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVE 120
           SGR QYVSADLR+K Q++K F+G EVVFHMAAP+SSINN++L YSVNV+G  NVIDAC+E
Sbjct: 63  SGRVQYVSADLRNKTQVVKGFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTTNVIDACIE 122

Query: 121 KNVKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLL 180
             VKRLIYTSS SVVFDG++G +NADE+LPY  KHND YSATKAEGEALILKANG +GLL
Sbjct: 123 VGVKRLIYTSSPSVVFDGVHGTLNADESLPYPPKHNDSYSATKAEGEALILKANGRSGLL 182

Query: 181 TCSLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERAL 240
           TC +RPS IFGPGD+L+VPSLV  ARAGKSKFIIG G+N YDFTYVENV HAHVCAERAL
Sbjct: 183 TCCIRPSSIFGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTYVENVVHAHVCAERAL 242

Query: 241 ASGGMIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWI 300
           ASGG +  KAAGQAYFITNMEPIKFWEF+S +L+GLGYERP IKIPA L+MPIA++V   
Sbjct: 243 ASGGEVCAKAAGQAYFITNMEPIKFWEFMSQLLEGLGYERPSIKIPASLMMPIAYLVELA 302

Query: 301 YTLLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQE-LKKTM 353
           Y LLGPYGM VP  TPSR+RLLSC RTF+SSKAKDRL Y+P+V LQE +K+T+
Sbjct: 303 YKLLGPYGMKVPVLTPSRVRLLSCNRTFDSSKAKDRLGYSPVVPLQEGIKRTI 355

BLAST of Csor.00g209060 vs. TAIR 10
Match: AT2G26260.2 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 )

HSP 1 Score: 412.1 bits (1058), Expect = 4.4e-115
Identity = 198/258 (76.74%), Postives = 225/258 (87.21%), Query Frame = 0

Query: 90  MAAPNSSINNYKLHYSVNVEGAKNVIDACVEKNVKRLIYTSSASVVFDGINGLVNADETL 149
           MAAP+SSINN++L YSVNV+G +NVIDACV+  VKRLIYTSS SVVFDG++G++N  E++
Sbjct: 1   MAAPDSSINNHQLQYSVNVQGTQNVIDACVDVGVKRLIYTSSPSVVFDGVHGILNGTESM 60

Query: 150 PYASKHNDFYSATKAEGEALILKANGLNGLLTCSLRPSGIFGPGDRLLVPSLVANARAGK 209
            Y  KHND YSATKAEGE LI+KANG NGLLTC +RPS IFGPGDRLLVPSLVA ARAGK
Sbjct: 61  AYPIKHNDSYSATKAEGEELIMKANGRNGLLTCCIRPSSIFGPGDRLLVPSLVAAARAGK 120

Query: 210 SKFIIGKGNNTYDFTYVENVGHAHVCAERALASGGMIAEKAAGQAYFITNMEPIKFWEFV 269
           SKFIIG GNN YDFTYVENV HAHVCAERALASGG ++ KAAGQAYFITNMEPIKFWEF+
Sbjct: 121 SKFIIGDGNNLYDFTYVENVAHAHVCAERALASGGDVSTKAAGQAYFITNMEPIKFWEFM 180

Query: 270 SLILDGLGYERPRIKIPAYLVMPIAHMVNWIYTLLGPYGMPVPQFTPSRIRLLSCCRTFN 329
           S +LDGLGYERP IKIPA+++MPIAH+V   Y +LGPYGM VPQ TPSR+RLLSC RTF+
Sbjct: 181 SQLLDGLGYERPSIKIPAFIMMPIAHLVELTYKVLGPYGMTVPQLTPSRVRLLSCSRTFD 240

Query: 330 SSKAKDRLFYTPIVSLQE 348
           S+KAKDRL Y P+V LQE
Sbjct: 241 STKAKDRLGYAPVVPLQE 258

BLAST of Csor.00g209060 vs. TAIR 10
Match: AT2G43420.1 (3-beta hydroxysteroid dehydrogenase/isomerase family protein )

HSP 1 Score: 283.1 bits (723), Expect = 3.1e-76
Identity = 158/344 (45.93%), Postives = 216/344 (62.79%), Query Frame = 0

Query: 5   SEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEKGILGEALRSGRA 64
           S  K CVV GGRGF  R LV  L+    ++VR+ D G ++ L+ S+   +L +AL SGRA
Sbjct: 10  SHLKTCVVLGGRGFIGRSLVSRLLRLGNWTVRVADSGHTLHLDESD--SLLEDALSSGRA 69

Query: 65  QYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVEKNVK 124
            Y   D+RDK Q++K  EG  VVF+M A +   ++Y   Y V V+G +NVI AC E  V+
Sbjct: 70  SYHCVDVRDKPQIVKVTEGSYVVFYMGATDLRSHDYFDCYKVIVQGTRNVISACRESGVR 129

Query: 125 RLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLLTCSL 184
           +LIY S+A VVFDG   + + DE+L    K     +  KA+ EALI  AN  +GLLTC+L
Sbjct: 130 KLIYNSTADVVFDGSQPIRDGDESLRRPLKFQSMLTDFKAQAEALIKLANNRDGLLTCAL 189

Query: 185 RPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERALASGG 244
           R S +FGPGD   VP LV  A++G +KFI+G G N  DFTY ENV HAH+CA +AL S  
Sbjct: 190 RSSIVFGPGDTEFVPFLVNLAKSGYAKFILGSGENISDFTYSENVSHAHICAVKALDSQ- 249

Query: 245 MIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWIYTLL 304
              E  AG+ +FITN++P++FW+FVS I++GLGY RP IK+P  LV+ +  ++ W +   
Sbjct: 250 --MEFVAGKEFFITNLKPVRFWDFVSHIVEGLGYPRPSIKLPVRLVLYVFSLLKWTHE-- 309

Query: 305 GPYGMPVPQFTPSRIRLL-SCCRTFNSSKAKDRLFYTPIVSLQE 348
              G+     T  +  LL S  RTFN + AK  L YTP+V+L++
Sbjct: 310 -KEGLGSNYDTAHQYALLASSTRTFNCNAAKKHLGYTPVVTLED 345

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q67ZE15.9e-14974.783beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 OS=Arabidopsis thalia... [more]
Q9FX011.3e-14873.943beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 OS=Arabidopsis thalia... [more]
A9X4U24.3e-7545.933beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 3 OS=Arabidopsis thalia... [more]
Q9R1J03.2e-6239.83Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Mus musculus OX=1... [more]
Q5PPL34.2e-6240.12Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Rattus norvegicus... [more]
Match NameE-valueIdentityDescription
KAG6599124.15.63e-259100.003beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2, partial [Cucurbita a... [more]
XP_022946821.15.69e-24899.713beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita moschata] >XP_0... [more]
XP_023546590.13.28e-24799.423beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita pepo subsp. pep... [more]
XP_022999664.17.67e-24699.143beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita maxima] >XP_022... [more]
KAA0064699.12.68e-23491.983beta-hydroxysteroid-dehydrogenase/decarboxylase isoform X1 [Cucumis melo var. m... [more]
Match NameE-valueIdentityDescription
A0A6J1G5422.75e-24899.71Reticulon-like protein OS=Cucurbita moschata OX=3662 GN=LOC111450770 PE=4 SV=1[more]
A0A6J1KG573.71e-24699.14Reticulon-like protein OS=Cucurbita maxima OX=3661 GN=LOC111493953 PE=4 SV=1[more]
A0A5D3BNL71.30e-23491.983beta-hydroxysteroid-dehydrogenase/decarboxylase isoform X1 OS=Cucumis melo var.... [more]
A0A1S3BDV06.95e-23393.04Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103488530 PE=4 SV=1[more]
A0A1S3BDP71.69e-23293.04Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103488530 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G26260.14.2e-15074.783beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 [more]
AT1G47290.19.4e-15073.943beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 [more]
AT1G47290.29.4e-15073.943beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 [more]
AT2G26260.24.4e-11576.743beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 [more]
AT2G43420.13.1e-7645.933-beta hydroxysteroid dehydrogenase/isomerase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 2..356
e-value: 2.5E-78
score: 265.6
NoneNo IPR availablePANTHERPTHR10366NAD DEPENDENT EPIMERASE/DEHYDRATASEcoord: 6..353
NoneNo IPR availablePANTHERPTHR10366:SF7253BETA-HYDROXYSTEROID-DEHYDROGENASE/DECARBOXYLASE ISOFORM 1coord: 6..353
IPR0022253-beta hydroxysteroid dehydrogenase/isomerasePFAMPF010733Beta_HSDcoord: 11..285
e-value: 2.9E-77
score: 259.4
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 7..352

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g209060.m01Csor.00g209060.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006694 steroid biosynthetic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003854 3-beta-hydroxy-delta5-steroid dehydrogenase activity
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor