Csor.00g208490 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g208490
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionPectate lyase
LocationCsor_Chr05: 7921998 .. 7923700 (+)
RNA-Seq ExpressionCsor.00g208490
SyntenyCsor.00g208490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGGGTTTAGAATCTTGTTCGTCTTCTCGTTGTTGTACTTTGTCGCGGCATATACCGATGCCAATGTCGATGTCGATGACCATGATGAATATTGGCTCACTCGTGCTAATGAGGCTAAGAAAGTAGCCCAAGATGCCTACAATCCTAAGCCAGAAAGCGTCACTGAGCATTTGAACGTCGCCGTCCGTCTGTAAGTAGATCCATAATAAAAAAAGTGTTTGTGAATAAATGAAACAATATGTACATCATGAACTGTCCTGTAACGAAAATATGGTTTGGCAGGGCGATGGTGGGTGACAATAGTACAAGAAGGAGTCTCAATAAATATAAAGGACCATGTTTGAAGACAAACCCGATCGATAGATGCTGGAGATGCGATAAAAATTGGGCTAACAATCGAAAGAAGCTAGCCGAGTGCCCGTTAGGGTTTGGCCACCAAACCATCGGCGGAAAGAGAGGACGCATCTACGTGGTGACCGACGCATCTGATGATAACTTGGTTGACCCCAAGCCCGGAACCCTCCGCCATGGCGTGATTCAAAAAGGTCCACTTTGGATCATTTTTGGACGTAGCATGGTCATCAGGCTAGCCCAAGAGCTGATGATCTCAAGCGACAAAACCATCGATGCTCGTGGCTTTAATGTTCACATTGCCTATGGCGCTGGCCTCACCATTCAATTCGCCCACAATGTTATCATCCATGGCCTTCACATCCATAATATCATCTCCAAATCCGGTGGCTATATTAGAGATTCCGTTGATCATTATGGATTTAGAACACAAAGCGATGGCGATGGTATCTCCATCTTTGGCTCTTCTCATATATGGATCGACCATGTTTCCATGTATGCATGCCAAGACGGCCTCATCGATGCCATCATGGGTTCCACCGGCATCACCATTTCCAATAGTCATTTCACTCATCACAACGAGGTATCTAATTATTTCATTTATGACATTCTGCTTTGTTTATGACATACCATTGTGGGAGAGTCATGATTCCTATCATGGAATCACACCATTGTGGGTTTGTCGTGCTTCCTAACGCGATATGGAATCACACCATTGTGGGTCCTAACGCGATATGGAATCACACCATTGTGGGTCCTAACGCGTTAGGGAATCACACCATTGTGGGTTTGTCGTGCTTCCTAACGCGTCAGGGAATCACACCATTGTGGGGTTGTCGTGCTTCCTAACACTGTATCATCTGTATATGAACAGGCGATGTTATTCGGAGCAAGCGATAGCTACAAAGAGGACGAGATCATGCAAATAACCCTCGCATTCAACCACTTCGGGAGGGGATTGGTGCAAAGAATGCCAAGGTGTAGATTTGGGTTCTTCCATGTGGTAAACAATGACTACACCCATTGGCTAATGTATGCCATCGGAGGTAGCCAACATCCAACCATCGTGAGCCAAGGCAACCGATTCATTGCGCCACAGAACATCAATGCAAAAACGATAACAAAGAGAGAGTATGCAGATGAGAGTGAGTGGAAGAAGTGGTCGTGGAGATCGGAAGGGGATTTGTTGTTGAATGGTGCGGTGTTTGTTGGGTCGGGAGACCAAACCAAAGGGAAGAAGAAGGACTTGTCGAGATATGACATGATTTCGTTCAAGCCAGGGTCTTATGTGAAGAGACTTGTACGCTTCGCGGGAACACTTGAATGTCGTGTAGGCAAACCATGCTAG

mRNA sequence

ATGGCCGGGTTTAGAATCTTGTTCGTCTTCTCGTTGTTGTACTTTGTCGCGGCATATACCGATGCCAATGTCGATGTCGATGACCATGATGAATATTGGCTCACTCGTGCTAATGAGGCTAAGAAAGTAGCCCAAGATGCCTACAATCCTAAGCCAGAAAGCGTCACTGAGCATTTGAACGTCGCCGTCCGTCTGGCGATGGTGGGTGACAATAGTACAAGAAGGAGTCTCAATAAATATAAAGGACCATGTTTGAAGACAAACCCGATCGATAGATGCTGGAGATGCGATAAAAATTGGGCTAACAATCGAAAGAAGCTAGCCGAGTGCCCGTTAGGGTTTGGCCACCAAACCATCGGCGGAAAGAGAGGACGCATCTACGTGGTGACCGACGCATCTGATGATAACTTGGTTGACCCCAAGCCCGGAACCCTCCGCCATGGCGTGATTCAAAAAGGTCCACTTTGGATCATTTTTGGACGTAGCATGGTCATCAGGCTAGCCCAAGAGCTGATGATCTCAAGCGACAAAACCATCGATGCTCGTGGCTTTAATGTTCACATTGCCTATGGCGCTGGCCTCACCATTCAATTCGCCCACAATGTTATCATCCATGGCCTTCACATCCATAATATCATCTCCAAATCCGGTGGCTATATTAGAGATTCCGTTGATCATTATGGATTTAGAACACAAAGCGATGGCGATGGTATCTCCATCTTTGGCTCTTCTCATATATGGATCGACCATGTTTCCATGTATGCATGCCAAGACGGCCTCATCGATGCCATCATGGGTTCCACCGGCATCACCATTTCCAATAGTCATTTCACTCATCACAACGAGGCGATGTTATTCGGAGCAAGCGATAGCTACAAAGAGGACGAGATCATGCAAATAACCCTCGCATTCAACCACTTCGGGAGGGGATTGGTGCAAAGAATGCCAAGGTGTAGATTTGGGTTCTTCCATGTGGTAAACAATGACTACACCCATTGGCTAATGTATGCCATCGGAGGTAGCCAACATCCAACCATCGTGAGCCAAGGCAACCGATTCATTGCGCCACAGAACATCAATGCAAAAACGATAACAAAGAGAGAGTATGCAGATGAGAGTGAGTGGAAGAAGTGGTCGTGGAGATCGGAAGGGGATTTGTTGTTGAATGGTGCGGTGTTTGTTGGGTCGGGAGACCAAACCAAAGGGAAGAAGAAGGACTTGTCGAGATATGACATGATTTCGTTCAAGCCAGGGTCTTATGTGAAGAGACTTGTACGCTTCGCGGGAACACTTGAATGTCGTGTAGGCAAACCATGCTAG

Coding sequence (CDS)

ATGGCCGGGTTTAGAATCTTGTTCGTCTTCTCGTTGTTGTACTTTGTCGCGGCATATACCGATGCCAATGTCGATGTCGATGACCATGATGAATATTGGCTCACTCGTGCTAATGAGGCTAAGAAAGTAGCCCAAGATGCCTACAATCCTAAGCCAGAAAGCGTCACTGAGCATTTGAACGTCGCCGTCCGTCTGGCGATGGTGGGTGACAATAGTACAAGAAGGAGTCTCAATAAATATAAAGGACCATGTTTGAAGACAAACCCGATCGATAGATGCTGGAGATGCGATAAAAATTGGGCTAACAATCGAAAGAAGCTAGCCGAGTGCCCGTTAGGGTTTGGCCACCAAACCATCGGCGGAAAGAGAGGACGCATCTACGTGGTGACCGACGCATCTGATGATAACTTGGTTGACCCCAAGCCCGGAACCCTCCGCCATGGCGTGATTCAAAAAGGTCCACTTTGGATCATTTTTGGACGTAGCATGGTCATCAGGCTAGCCCAAGAGCTGATGATCTCAAGCGACAAAACCATCGATGCTCGTGGCTTTAATGTTCACATTGCCTATGGCGCTGGCCTCACCATTCAATTCGCCCACAATGTTATCATCCATGGCCTTCACATCCATAATATCATCTCCAAATCCGGTGGCTATATTAGAGATTCCGTTGATCATTATGGATTTAGAACACAAAGCGATGGCGATGGTATCTCCATCTTTGGCTCTTCTCATATATGGATCGACCATGTTTCCATGTATGCATGCCAAGACGGCCTCATCGATGCCATCATGGGTTCCACCGGCATCACCATTTCCAATAGTCATTTCACTCATCACAACGAGGCGATGTTATTCGGAGCAAGCGATAGCTACAAAGAGGACGAGATCATGCAAATAACCCTCGCATTCAACCACTTCGGGAGGGGATTGGTGCAAAGAATGCCAAGGTGTAGATTTGGGTTCTTCCATGTGGTAAACAATGACTACACCCATTGGCTAATGTATGCCATCGGAGGTAGCCAACATCCAACCATCGTGAGCCAAGGCAACCGATTCATTGCGCCACAGAACATCAATGCAAAAACGATAACAAAGAGAGAGTATGCAGATGAGAGTGAGTGGAAGAAGTGGTCGTGGAGATCGGAAGGGGATTTGTTGTTGAATGGTGCGGTGTTTGTTGGGTCGGGAGACCAAACCAAAGGGAAGAAGAAGGACTTGTCGAGATATGACATGATTTCGTTCAAGCCAGGGTCTTATGTGAAGAGACTTGTACGCTTCGCGGGAACACTTGAATGTCGTGTAGGCAAACCATGCTAG

Protein sequence

MAGFRILFVFSLLYFVAAYTDANVDVDDHDEYWLTRANEAKKVAQDAYNPKPESVTEHLNVAVRLAMVGDNSTRRSLNKYKGPCLKTNPIDRCWRCDKNWANNRKKLAECPLGFGHQTIGGKRGRIYVVTDASDDNLVDPKPGTLRHGVIQKGPLWIIFGRSMVIRLAQELMISSDKTIDARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSDGDGISIFGSSHIWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKEDEIMQITLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPQNINAKTITKREYADESEWKKWSWRSEGDLLLNGAVFVGSGDQTKGKKKDLSRYDMISFKPGSYVKRLVRFAGTLECRVGKPC
Homology
BLAST of Csor.00g208490 vs. ExPASy Swiss-Prot
Match: P15722 (Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 1.6e-159
Identity = 276/443 (62.30%), Postives = 323/443 (72.91%), Query Frame = 0

Query: 8   FVFSLLYFVAAYTDANVDVDDHDEYWLTRANEAKKVAQDAYNPKPESVTEHLNVAVRLAM 67
           F+F  + F          +  +DE W  RA EAK+ A++ Y P PE+VT   N  +R  M
Sbjct: 10  FLFLCITFATIIPSLMAHIGHYDEVWRRRAEEAKEYARNIYEPHPENVTLAFNQKLRDTM 69

Query: 68  --------VGDNSTRRSL--NKYKGPCLKTNPIDRCWRCDKNWANNRKKLAECPLGFGHQ 127
                     +NSTRR L   KY GPC+ TNPID+CWRCD NWA+NRKKLA+C +GFG +
Sbjct: 70  KELKKVKGTHNNSTRRGLGTKKYTGPCMVTNPIDKCWRCDPNWADNRKKLADCAMGFGSK 129

Query: 128 TIGGKRGRIYVVTDASDDNLVDPKPGTLRHGVIQKGPLWIIFGRSMVIRLAQELMISSDK 187
            IGGK G  YVVTD SDD   DPKPGTLRH VIQK PLWIIF R M IRL QE+++ SDK
Sbjct: 130 AIGGKDGEFYVVTDNSDD-YNDPKPGTLRHAVIQKEPLWIIFKRGMNIRLHQEMIMQSDK 189

Query: 188 TIDARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSDGDG 247
           TIDARG NVHI  GAG+T+Q+  NVIIHGLHIH+I+  +GG +RD+VDH G RT+SDGDG
Sbjct: 190 TIDARGVNVHITKGAGITLQYIKNVIIHGLHIHDIVEGNGGMVRDAVDHIGIRTKSDGDG 249

Query: 248 ISIFGSSHIWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKEDEI 307
           ISIFG+S+IWIDHVSM  C DGLIDA+ GSTGITISN HFT HNE MLFGASDS   D++
Sbjct: 250 ISIFGASYIWIDHVSMQRCYDGLIDAVEGSTGITISNGHFTDHNEVMLFGASDSSSIDQV 309

Query: 308 MQITLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPQ 367
           MQITLAFNHFG+ L+QRMPRCR+G+ HVVNNDYTHW MYAIGGS HPTI+ QGNRFIAP 
Sbjct: 310 MQITLAFNHFGKRLIQRMPRCRWGYIHVVNNDYTHWNMYAIGGSMHPTIIHQGNRFIAPP 369

Query: 368 NINAKTITKREYADESEWKKWSWRSEGDLLLNGAVFVGSGD-QTKGKKKDLSRYDMISFK 427
           +I  K +TKREY  ES W +W+WRSEG+L +NGA F  SGD +   K KDL  YD IS  
Sbjct: 370 DIFKKQVTKREYNPESVWMQWTWRSEGNLFMNGAYFTESGDPEWSSKHKDL--YDGISAA 429

Query: 428 PGSYVKRLVRFAGTLECRVGKPC 440
           P   V  + RFAG L C+ GKPC
Sbjct: 430 PAEDVTWMTRFAGVLGCKPGKPC 449

BLAST of Csor.00g208490 vs. ExPASy Swiss-Prot
Match: P40973 (Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 2.7e-159
Identity = 270/433 (62.36%), Postives = 328/433 (75.75%), Query Frame = 0

Query: 8   FVFSLLYFVAAYTDANVDVDDHDEYWLTRANEAKKVAQDAYNPKPESVTEHLNVAVRLAM 67
           F++SLL+F +A   ++ ++ + DEYW  ++  A+  A+ AY P PE VT H N AV  + 
Sbjct: 7   FLYSLLFFASAAL-SSANIAEFDEYWQKKSKVAQAKAKKAYTPHPEEVTNHFNKAVHSSF 66

Query: 68  VGDNSTRRSLNKYK-GPCLKTNPIDRCWRCDKNWANNRKKLAECPLGFGHQTIGGKRGRI 127
            G NSTRR+L   K G CL TNPIDRCWRC KNW+ NRK L +C  GFG +T GG  G I
Sbjct: 67  EG-NSTRRNLRTNKLGQCLATNPIDRCWRCKKNWSANRKDLVKCVKGFGRKTTGGAAGEI 126

Query: 128 YVVTDASDDNLVDPKPGTLRHGVIQKGPLWIIFGRSMVIRLAQELMISSDKTIDARGFNV 187
           YVVTD SDD+L DPK GTLR GVIQ  PLWIIFG+SMVIRL QEL+I++DKTID RG NV
Sbjct: 127 YVVTDPSDDSLTDPKFGTLRWGVIQDRPLWIIFGKSMVIRLKQELIINNDKTIDGRGANV 186

Query: 188 HIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSDGDGISIFGSSHI 247
            IA GA LT+QF HNVIIHG+HIH+I    GG IRDS  H G RT+SDGDGISI GSS+I
Sbjct: 187 QIAGGAQLTVQFVHNVIIHGIHIHDIKPGEGGLIRDSEKHSGIRTRSDGDGISIIGSSNI 246

Query: 248 WIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKEDEIMQITLAFNH 307
           WIDHVS+  C DGLID I+GST ITISN H T H++ ML GASD+Y +DEIMQ+T+AFNH
Sbjct: 247 WIDHVSLARCSDGLIDVILGSTAITISNCHLTEHDDVMLLGASDTYTQDEIMQVTVAFNH 306

Query: 308 FGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPQNINAKTITK 367
           FGRGLVQRMPRCR+GF HVVNNDYTHW+MYA+GGSQHPTI+SQGNR+IAP    AK +TK
Sbjct: 307 FGRGLVQRMPRCRYGFVHVVNNDYTHWIMYAVGGSQHPTIISQGNRYIAPHIEAAKEVTK 366

Query: 368 REYADESEWKKWSWRSEGDLLLNGAVFVGSGDQTKGKKKDLSRYDMISFKPGSYVKRLVR 427
           R+YA+ +EW KW+W+S+GDL ++GA FV SG   + K    S+ D+I  KPG++V+RL R
Sbjct: 367 RDYAEPAEWSKWTWKSQGDLFVSGAFFVESGGPFENK---YSKKDLIKAKPGTFVQRLTR 426

Query: 428 FAGTLECRVGKPC 440
           F+G L C+    C
Sbjct: 427 FSGALNCKENMEC 434

BLAST of Csor.00g208490 vs. ExPASy Swiss-Prot
Match: Q9SRH4 (Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 1.0e-150
Identity = 258/455 (56.70%), Postives = 322/455 (70.77%), Query Frame = 0

Query: 22  ANVDVDDHDEYWLTRANEAKKVAQDAYNPKPESVTEHLNV-------------------- 81
           ANV  D+ DEYW+ +ANEA+K    AY+P P  + +H +                     
Sbjct: 24  ANV-ADETDEYWVNKANEARKHTLMAYHPDPYEIVDHFHERHYDNSTDVEGTEEEKAVAS 83

Query: 82  ----AVRLAMVGDNSTRRSL------------NKYKGPCLKTNPIDRCWRCDKNWANNRK 141
                + +     NSTRRSL            +K  GPC  +NPID+CWRC  +WA  RK
Sbjct: 84  EEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTGPCTASNPIDKCWRCQPDWARRRK 143

Query: 142 KLAECPLGFGHQTIGGKRGRIYVVTDASDDNLVDPKPGTLRHGVIQKGPLWIIFGRSMVI 201
           KL  C  GFG++T GGKRGRIYVVT   DD++V+P+PGTLRH VIQK PLWI+F   M I
Sbjct: 144 KLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTLRHAVIQKEPLWIVFKHDMSI 203

Query: 202 RLAQELMISSDKTIDARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVD 261
           RL+QELMI+SDKTIDARG NVHIAYGAG+T+Q+ HN+IIHGLH+H+I+  SGG IRDS++
Sbjct: 204 RLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNIIIHGLHVHHIVKSSGGLIRDSIN 263

Query: 262 HYGFRTQSDGDGISIFGSSHIWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAML 321
           H+G R ++DGDGISIFG+++IW+DH+SM  CQDGLIDAIMGST ITISNSHFTHHN+ ML
Sbjct: 264 HFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLIDAIMGSTAITISNSHFTHHNDVML 323

Query: 322 FGASDSYKEDEIMQITLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPT 381
            GA ++  +D+ MQ+T+A+NHFG+GLVQRMPR R+GF HVVNNDYTHW +YAIGGSQ PT
Sbjct: 324 LGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHVVNNDYTHWELYAIGGSQGPT 383

Query: 382 IVSQGNRFIA-PQNINAKTITKREYADESEWKKWSWRSEGDLLLNGAVFVGSGDQTKGKK 440
           I+S GNRFIA P   + + +TKR+YA ESEWK W+WRSE D+ +N A F  SG+     K
Sbjct: 384 ILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWRSEKDVFMNNAYFRQSGN--PHFK 443

BLAST of Csor.00g208490 vs. ExPASy Swiss-Prot
Match: Q9M9S2 (Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1)

HSP 1 Score: 523.9 bits (1348), Expect = 1.8e-147
Identity = 261/454 (57.49%), Postives = 316/454 (69.60%), Query Frame = 0

Query: 1   MAGFRILFVFSLLYFVAAYTDANVDVDDHDEYWLTRANEAKKVAQDAYNPKPESVTEHLN 60
           + G+  +F  S L  VA     NV V   D YW  R ++A K    +Y+P P +VT H N
Sbjct: 8   LGGYVFVFFSSFLAIVAPQVRGNVAV--FDSYWTQRQSDALKQTIGSYDPHPLNVTNHFN 67

Query: 61  VAVRLAMVGD---NSTRRSL---------NKYKGPCLKTNPIDRCWRCDKNWANNRKKLA 120
             V +A+      N TRR L         +K  G CL  NPID CWRCD+NWANNRKKLA
Sbjct: 68  YHVNIAVDASESRNDTRRELTQVRSGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLA 127

Query: 121 ECPLGFGHQTIGGKRGRIYVVTDASDDNLVDPKPGTLRHGVIQKGPLWIIFGRSMVIRLA 180
           +C LGFG +T GGK G IYVV DASD++L++PKPGTLRH V + GPLWIIF RSM+I+L 
Sbjct: 128 DCVLGFGRRTTGGKDGPIYVVKDASDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQ 187

Query: 181 QELMISSDKTIDARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYG 240
           QELMI+SDKTID RG  V+I  GAGLT+QF +NVIIH +++ +I+  +GG IRDS  H G
Sbjct: 188 QELMITSDKTIDGRGARVYIMEGAGLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIG 247

Query: 241 FRTQSDGDGISIFGSSHIWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGA 300
            RT+SDGDGIS+FG+++IWIDHVSM  C DG+IDAI GST +TISNSHFT H E MLFGA
Sbjct: 248 LRTKSDGDGISLFGATNIWIDHVSMTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGA 307

Query: 301 SDSYKEDEIMQITLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVS 360
            D +  D+ MQIT+AFNHFG+ L QRMPRCR+G  HVVNNDYTHW MYAIGG+ +PTI+S
Sbjct: 308 RDEHVIDKKMQITVAFNHFGKRLEQRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIIS 367

Query: 361 QGNRFIAPQNINAKTITKREYADESEWKKWSWRSEGDLLLNGAVFVGSGDQ---TKGKKK 420
           QGNRFIAP N  AK ITKREY    EWK W+W+SEGD  LNGA FV SG     +   K 
Sbjct: 368 QGNRFIAPPNEEAKQITKREYTPYGEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKT 427

Query: 421 DLSRYDMISFKPGSYVKRLVRFAGTLECRVGKPC 440
            L     I  KPG+ V++L   AG L C++G+ C
Sbjct: 428 PLPNKFTIRPKPGTMVRKLTMDAGVLGCKLGEAC 459

BLAST of Csor.00g208490 vs. ExPASy Swiss-Prot
Match: Q9LFP5 (Probable pectate lyase 19 OS=Arabidopsis thaliana OX=3702 GN=At5g15110 PE=2 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 5.2e-147
Identity = 255/470 (54.26%), Postives = 324/470 (68.94%), Query Frame = 0

Query: 5   RILFVFSLLYFVAAYTDANVDVDDHDEYWLTRANEAKKVAQDAYNPKPESVTEHLNVA-- 64
           +++F F +   +        ++ + DEYW  RA+EA++    AY+  P  + +H +    
Sbjct: 8   KLMFTFCIAVLIPTIRG---NISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHERHY 67

Query: 65  ------------------------VRLAMVGDNSTRRSL--------NKYKGPCLKTNPI 124
                                   + +     NSTRRSL        +K KGPC  +NPI
Sbjct: 68  DNSTDVTTPEEDGDAKPEEEEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASNPI 127

Query: 125 DRCWRCDKNWANNRKKLAECPLGFGHQTIGGKRGRIYVVTDASDDNLVDPKPGTLRHGVI 184
           D+CWRC  +WA  RKKL  C  GFGH+T GGKRGRIYVVT   D+++V+PKPGTLRH VI
Sbjct: 128 DKCWRCRSDWAKRRKKLTRCVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHAVI 187

Query: 185 QKGPLWIIFGRSMVIRLAQELMISSDKTIDARGFNVHIAYGAGLTIQFAHNVIIHGLHIH 244
           QK PLWIIF   M IRL QEL+I+S KTIDARG NVH+A+GAG+T+QF  NVIIHGLHIH
Sbjct: 188 QKEPLWIIFKNDMSIRLNQELLINSHKTIDARGANVHVAHGAGITMQFVKNVIIHGLHIH 247

Query: 245 NIISKSGGYIRDSVDHYGFRTQSDGDGISIFGSSHIWIDHVSMYACQDGLIDAIMGSTGI 304
           +I   SGG IRDSVDH+G RT++DGDG+SI+GSS+IW+DH+SM  CQDGLIDAI+GSTGI
Sbjct: 248 HISESSGGMIRDSVDHFGMRTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGSTGI 307

Query: 305 TISNSHFTHHNEAMLFGASDSYKEDEIMQITLAFNHFGRGLVQRMPRCRFGFFHVVNNDY 364
           TISNSHFTHHN+ ML GA ++ + D+ MQ+T+A+NHFG+GLVQRMPR R+GF HVVNNDY
Sbjct: 308 TISNSHFTHHNDVMLLGAQNTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNNDY 367

Query: 365 THWLMYAIGGSQHPTIVSQGNRFIA-PQNINAKTITKREYADESEWKKWSWRSEGDLLLN 424
           THW +YAIGGSQ PTI+S GNRFIA P   + + +TKR+YA E EWK W+WRS+ D+ +N
Sbjct: 368 THWELYAIGGSQGPTILSHGNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVFMN 427

Query: 425 GAVFVGSGDQTKGKKKDLSRYDMISFKPGSYVKRLVRFAGTLECRVGKPC 440
           GA F  SG+     K   +R  MI  K G  V +L ++AG L+CRVG+ C
Sbjct: 428 GAYFRQSGNPQ--YKCAHTRQQMIKPKNGLAVSKLTKYAGALDCRVGRRC 472

BLAST of Csor.00g208490 vs. NCBI nr
Match: XP_022946578.1 (pectate lyase-like [Cucurbita moschata] >KAG6599067.1 putative pectate lyase P59, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 919 bits (2374), Expect = 0.0
Identity = 439/439 (100.00%), Postives = 439/439 (100.00%), Query Frame = 0

Query: 1   MAGFRILFVFSLLYFVAAYTDANVDVDDHDEYWLTRANEAKKVAQDAYNPKPESVTEHLN 60
           MAGFRILFVFSLLYFVAAYTDANVDVDDHDEYWLTRANEAKKVAQDAYNPKPESVTEHLN
Sbjct: 1   MAGFRILFVFSLLYFVAAYTDANVDVDDHDEYWLTRANEAKKVAQDAYNPKPESVTEHLN 60

Query: 61  VAVRLAMVGDNSTRRSLNKYKGPCLKTNPIDRCWRCDKNWANNRKKLAECPLGFGHQTIG 120
           VAVRLAMVGDNSTRRSLNKYKGPCLKTNPIDRCWRCDKNWANNRKKLAECPLGFGHQTIG
Sbjct: 61  VAVRLAMVGDNSTRRSLNKYKGPCLKTNPIDRCWRCDKNWANNRKKLAECPLGFGHQTIG 120

Query: 121 GKRGRIYVVTDASDDNLVDPKPGTLRHGVIQKGPLWIIFGRSMVIRLAQELMISSDKTID 180
           GKRGRIYVVTDASDDNLVDPKPGTLRHGVIQKGPLWIIFGRSMVIRLAQELMISSDKTID
Sbjct: 121 GKRGRIYVVTDASDDNLVDPKPGTLRHGVIQKGPLWIIFGRSMVIRLAQELMISSDKTID 180

Query: 181 ARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSDGDGISI 240
           ARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSDGDGISI
Sbjct: 181 ARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSDGDGISI 240

Query: 241 FGSSHIWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKEDEIMQI 300
           FGSSHIWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKEDEIMQI
Sbjct: 241 FGSSHIWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKEDEIMQI 300

Query: 301 TLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPQNIN 360
           TLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPQNIN
Sbjct: 301 TLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPQNIN 360

Query: 361 AKTITKREYADESEWKKWSWRSEGDLLLNGAVFVGSGDQTKGKKKDLSRYDMISFKPGSY 420
           AKTITKREYADESEWKKWSWRSEGDLLLNGAVFVGSGDQTKGKKKDLSRYDMISFKPGSY
Sbjct: 361 AKTITKREYADESEWKKWSWRSEGDLLLNGAVFVGSGDQTKGKKKDLSRYDMISFKPGSY 420

Query: 421 VKRLVRFAGTLECRVGKPC 439
           VKRLVRFAGTLECRVGKPC
Sbjct: 421 VKRLVRFAGTLECRVGKPC 439

BLAST of Csor.00g208490 vs. NCBI nr
Match: XP_023523501.1 (pectate lyase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 891 bits (2303), Expect = 0.0
Identity = 427/439 (97.27%), Postives = 431/439 (98.18%), Query Frame = 0

Query: 1   MAGFRILFVFSLLYFVAAYTDANVDVDDHDEYWLTRANEAKKVAQDAYNPKPESVTEHLN 60
           MAGFR LFVFSLLYFVAA TDANVD  DHDEYWLTRANEAKKVAQDAYNP PESVTEHLN
Sbjct: 1   MAGFRTLFVFSLLYFVAACTDANVD--DHDEYWLTRANEAKKVAQDAYNPNPESVTEHLN 60

Query: 61  VAVRLAMVGDNSTRRSLNKYKGPCLKTNPIDRCWRCDKNWANNRKKLAECPLGFGHQTIG 120
           VAV LA+VGDNSTRRSLNKYKGPCLKTNPIDRCWRCDKNWA NRKKLAECPLGFGHQTIG
Sbjct: 61  VAVHLAIVGDNSTRRSLNKYKGPCLKTNPIDRCWRCDKNWAKNRKKLAECPLGFGHQTIG 120

Query: 121 GKRGRIYVVTDASDDNLVDPKPGTLRHGVIQKGPLWIIFGRSMVIRLAQELMISSDKTID 180
           GKRGRIYVVTDASDDNLVDPKPGTLR+GVIQKGPLWIIFGRSMVIRLAQELMISSDKTID
Sbjct: 121 GKRGRIYVVTDASDDNLVDPKPGTLRYGVIQKGPLWIIFGRSMVIRLAQELMISSDKTID 180

Query: 181 ARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSDGDGISI 240
           ARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSDGDGISI
Sbjct: 181 ARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSDGDGISI 240

Query: 241 FGSSHIWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKEDEIMQI 300
           FGSSH+WIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKEDEIMQI
Sbjct: 241 FGSSHVWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKEDEIMQI 300

Query: 301 TLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPQNIN 360
           TLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPQNIN
Sbjct: 301 TLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPQNIN 360

Query: 361 AKTITKREYADESEWKKWSWRSEGDLLLNGAVFVGSGDQTKGKKKDLSRYDMISFKPGSY 420
           AKTITKREYADESEWKKWSWRSEGDL LNGAVFVGSGDQTKGKKKDL+RYDMISFKPGSY
Sbjct: 361 AKTITKREYADESEWKKWSWRSEGDLFLNGAVFVGSGDQTKGKKKDLTRYDMISFKPGSY 420

Query: 421 VKRLVRFAGTLECRVGKPC 439
           VKRLVRFAGTLECRVGKPC
Sbjct: 421 VKRLVRFAGTLECRVGKPC 437

BLAST of Csor.00g208490 vs. NCBI nr
Match: XP_022999690.1 (pectate lyase-like [Cucurbita maxima])

HSP 1 Score: 886 bits (2289), Expect = 0.0
Identity = 426/439 (97.04%), Postives = 433/439 (98.63%), Query Frame = 0

Query: 1   MAGFRILFVFSLLYFVAAYTDANVDVDDHDEYWLTRANEAKKVAQDAYNPKPESVTEHLN 60
           MAGFRILFVFSL YF AAY  A+VDVDDHDEYWLTRANEAK+VA+DAYNP PESVTEHLN
Sbjct: 1   MAGFRILFVFSL-YFFAAY--ASVDVDDHDEYWLTRANEAKEVAKDAYNPNPESVTEHLN 60

Query: 61  VAVRLAMVGDNSTRRSLNKYKGPCLKTNPIDRCWRCDKNWANNRKKLAECPLGFGHQTIG 120
           VAVRLAMVGDNSTRRSLNKYKGPCLKTNPIDRCWRCDKNWANNRKKLAECPLGFGHQTIG
Sbjct: 61  VAVRLAMVGDNSTRRSLNKYKGPCLKTNPIDRCWRCDKNWANNRKKLAECPLGFGHQTIG 120

Query: 121 GKRGRIYVVTDASDDNLVDPKPGTLRHGVIQKGPLWIIFGRSMVIRLAQELMISSDKTID 180
           GKRGRIYVVTDASDDNLVDPK GTLR+GVIQKGPLWIIFGRSMVIRLAQELMISSDKTID
Sbjct: 121 GKRGRIYVVTDASDDNLVDPKLGTLRYGVIQKGPLWIIFGRSMVIRLAQELMISSDKTID 180

Query: 181 ARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSDGDGISI 240
           ARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSDGDGISI
Sbjct: 181 ARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSDGDGISI 240

Query: 241 FGSSHIWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKEDEIMQI 300
           FGSSH+WIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKEDEIMQI
Sbjct: 241 FGSSHVWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKEDEIMQI 300

Query: 301 TLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPQNIN 360
           TLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPQNIN
Sbjct: 301 TLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPQNIN 360

Query: 361 AKTITKREYADESEWKKWSWRSEGDLLLNGAVFVGSGDQTKGKKKDLSRYDMISFKPGSY 420
           AKTITKREYADESEWKKWSWRSEGDLL+NGAVFVGSGDQTKGKKKDLSRYDMIS+KPGSY
Sbjct: 361 AKTITKREYADESEWKKWSWRSEGDLLMNGAVFVGSGDQTKGKKKDLSRYDMISYKPGSY 420

Query: 421 VKRLVRFAGTLECRVGKPC 439
           VKRLVRFAGTLECRVGKPC
Sbjct: 421 VKRLVRFAGTLECRVGKPC 436

BLAST of Csor.00g208490 vs. NCBI nr
Match: KAG7030004.1 (putative pectate lyase P59 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 884 bits (2285), Expect = 0.0
Identity = 427/438 (97.49%), Postives = 428/438 (97.72%), Query Frame = 0

Query: 1   MAGFRILFVFSLLYFVAAYTDANVDVDDHDEYWLTRANEAKKVAQDAYNPKPESVTEHLN 60
           MAGFRILFVFSLLYFVAAYTDANVDVDDHDEYWLTRANEAKKVAQDAYNPKPESVTEHLN
Sbjct: 1   MAGFRILFVFSLLYFVAAYTDANVDVDDHDEYWLTRANEAKKVAQDAYNPKPESVTEHLN 60

Query: 61  VAVRLAMVGDNSTRRSLNKYKGPCLKTNPIDRCWRCDKNWANNRKKLAECPLGFGHQTIG 120
           VAVRLAMVGDNSTRRSLNKYKGPCLKTNPIDRCWRCDKNWANNRKKLAECPLGFGHQTIG
Sbjct: 61  VAVRLAMVGDNSTRRSLNKYKGPCLKTNPIDRCWRCDKNWANNRKKLAECPLGFGHQTIG 120

Query: 121 GKRGRIYVVTDASDDNLVDPKPGTLRHGVIQKGPLWIIFGRSMVIRLAQELMISSDKTID 180
           GKRG IYVVTDASDDNLVDPKPGTLRHGVIQKGPLWIIFGRSMVIRLAQELMISSDKTID
Sbjct: 121 GKRGHIYVVTDASDDNLVDPKPGTLRHGVIQKGPLWIIFGRSMVIRLAQELMISSDKTID 180

Query: 181 ARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSDGDGISI 240
           ARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSDGDGISI
Sbjct: 181 ARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSDGDGISI 240

Query: 241 FGSSHIWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKEDEIMQI 300
           FGSSHIWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKEDEIMQI
Sbjct: 241 FGSSHIWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKEDEIMQI 300

Query: 301 TLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPQNIN 360
           TLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPQNIN
Sbjct: 301 TLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPQNIN 360

Query: 361 AKTITKREYADESEWKKWSWRSEGDLLLNGAVFVGSGDQTKGKKKDLSRYDMISFKPGSY 420
           AKTITKREYADESEWKKWSWRSEGDLLLNGAVFVGSGDQTKGKKKDLSRYDMISFKPGSY
Sbjct: 361 AKTITKREYADESEWKKWSWRSEGDLLLNGAVFVGSGDQTKGKKKDLSRYDMISFKPGSY 420

Query: 421 VKRLVRFAGTLECRVGKP 438
           VKRL   +  L  R G P
Sbjct: 421 VKRL---STKLARREGSP 435

BLAST of Csor.00g208490 vs. NCBI nr
Match: XP_008456459.1 (PREDICTED: pectate lyase-like [Cucumis melo])

HSP 1 Score: 767 bits (1980), Expect = 8.94e-278
Identity = 368/439 (83.83%), Postives = 394/439 (89.75%), Query Frame = 0

Query: 1   MAGFRILFVFSLLYFVAAYTDANVDVDDHDEYWLTRANEAKKVAQDAYNPKPESVTEHLN 60
           MAGF  LFV SLL+FV A  +ANVD    DEYW TRA EAKKVA+ A+NP PE VT++LN
Sbjct: 1   MAGFSTLFVCSLLFFVVA--NANVD---EDEYWNTRATEAKKVAKSAFNPNPEIVTDNLN 60

Query: 61  VAVRLAMVGDNSTRRSLNKYKGPCLKTNPIDRCWRCDKNWANNRKKLAECPLGFGHQTIG 120
            AV  AMVG NSTRRSL KY+GPCL TNPIDRCWRCD+NWA NRKKLAECPLGFGHQTIG
Sbjct: 61  AAVSRAMVGRNSTRRSLKKYRGPCLATNPIDRCWRCDRNWAKNRKKLAECPLGFGHQTIG 120

Query: 121 GKRGRIYVVTDASDDNLVDPKPGTLRHGVIQKGPLWIIFGRSMVIRLAQELMISSDKTID 180
           GKRG IYVVTDASDD+LV+PKPGTLR+GVIQKGPLWIIFGRSM+IRL+QELMISSDKTID
Sbjct: 121 GKRGPIYVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTID 180

Query: 181 ARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSDGDGISI 240
           ARG NVHIA+GAGLTIQF +NVIIH LHIH+IISKSGG IRDSVDHYGFRTQSDGDGISI
Sbjct: 181 ARGANVHIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFRTQSDGDGISI 240

Query: 241 FGSSHIWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKEDEIMQI 300
           FGSSH+WIDHVSM  C+DGLIDAIMGST ITISN HFTHHNE MLFGASDSY EDEIMQ+
Sbjct: 241 FGSSHVWIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQV 300

Query: 301 TLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPQNIN 360
           TLAFNHFGRGLVQRMPRCR+GFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAP N N
Sbjct: 301 TLAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPN 360

Query: 361 AKTITKREYADESEWKKWSWRSEGDLLLNGAVFVGSGDQTKGKKKDLSRYDMISFKPGSY 420
           AK +TKREYA E EWK WSWRSEGD L+NGAVF+ SGDQTKGKKK LSRYDMISFKPG+Y
Sbjct: 361 AKQVTKREYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQTKGKKKGLSRYDMISFKPGTY 420

Query: 421 VKRLVRFAGTLECRVGKPC 439
           V+RL RF+GTLEC  GKPC
Sbjct: 421 VRRLARFSGTLECTPGKPC 434

BLAST of Csor.00g208490 vs. ExPASy TrEMBL
Match: A0A6J1G495 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111450600 PE=3 SV=1)

HSP 1 Score: 919 bits (2374), Expect = 0.0
Identity = 439/439 (100.00%), Postives = 439/439 (100.00%), Query Frame = 0

Query: 1   MAGFRILFVFSLLYFVAAYTDANVDVDDHDEYWLTRANEAKKVAQDAYNPKPESVTEHLN 60
           MAGFRILFVFSLLYFVAAYTDANVDVDDHDEYWLTRANEAKKVAQDAYNPKPESVTEHLN
Sbjct: 1   MAGFRILFVFSLLYFVAAYTDANVDVDDHDEYWLTRANEAKKVAQDAYNPKPESVTEHLN 60

Query: 61  VAVRLAMVGDNSTRRSLNKYKGPCLKTNPIDRCWRCDKNWANNRKKLAECPLGFGHQTIG 120
           VAVRLAMVGDNSTRRSLNKYKGPCLKTNPIDRCWRCDKNWANNRKKLAECPLGFGHQTIG
Sbjct: 61  VAVRLAMVGDNSTRRSLNKYKGPCLKTNPIDRCWRCDKNWANNRKKLAECPLGFGHQTIG 120

Query: 121 GKRGRIYVVTDASDDNLVDPKPGTLRHGVIQKGPLWIIFGRSMVIRLAQELMISSDKTID 180
           GKRGRIYVVTDASDDNLVDPKPGTLRHGVIQKGPLWIIFGRSMVIRLAQELMISSDKTID
Sbjct: 121 GKRGRIYVVTDASDDNLVDPKPGTLRHGVIQKGPLWIIFGRSMVIRLAQELMISSDKTID 180

Query: 181 ARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSDGDGISI 240
           ARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSDGDGISI
Sbjct: 181 ARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSDGDGISI 240

Query: 241 FGSSHIWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKEDEIMQI 300
           FGSSHIWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKEDEIMQI
Sbjct: 241 FGSSHIWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKEDEIMQI 300

Query: 301 TLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPQNIN 360
           TLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPQNIN
Sbjct: 301 TLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPQNIN 360

Query: 361 AKTITKREYADESEWKKWSWRSEGDLLLNGAVFVGSGDQTKGKKKDLSRYDMISFKPGSY 420
           AKTITKREYADESEWKKWSWRSEGDLLLNGAVFVGSGDQTKGKKKDLSRYDMISFKPGSY
Sbjct: 361 AKTITKREYADESEWKKWSWRSEGDLLLNGAVFVGSGDQTKGKKKDLSRYDMISFKPGSY 420

Query: 421 VKRLVRFAGTLECRVGKPC 439
           VKRLVRFAGTLECRVGKPC
Sbjct: 421 VKRLVRFAGTLECRVGKPC 439

BLAST of Csor.00g208490 vs. ExPASy TrEMBL
Match: A0A6J1KG97 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111493968 PE=3 SV=1)

HSP 1 Score: 886 bits (2289), Expect = 0.0
Identity = 426/439 (97.04%), Postives = 433/439 (98.63%), Query Frame = 0

Query: 1   MAGFRILFVFSLLYFVAAYTDANVDVDDHDEYWLTRANEAKKVAQDAYNPKPESVTEHLN 60
           MAGFRILFVFSL YF AAY  A+VDVDDHDEYWLTRANEAK+VA+DAYNP PESVTEHLN
Sbjct: 1   MAGFRILFVFSL-YFFAAY--ASVDVDDHDEYWLTRANEAKEVAKDAYNPNPESVTEHLN 60

Query: 61  VAVRLAMVGDNSTRRSLNKYKGPCLKTNPIDRCWRCDKNWANNRKKLAECPLGFGHQTIG 120
           VAVRLAMVGDNSTRRSLNKYKGPCLKTNPIDRCWRCDKNWANNRKKLAECPLGFGHQTIG
Sbjct: 61  VAVRLAMVGDNSTRRSLNKYKGPCLKTNPIDRCWRCDKNWANNRKKLAECPLGFGHQTIG 120

Query: 121 GKRGRIYVVTDASDDNLVDPKPGTLRHGVIQKGPLWIIFGRSMVIRLAQELMISSDKTID 180
           GKRGRIYVVTDASDDNLVDPK GTLR+GVIQKGPLWIIFGRSMVIRLAQELMISSDKTID
Sbjct: 121 GKRGRIYVVTDASDDNLVDPKLGTLRYGVIQKGPLWIIFGRSMVIRLAQELMISSDKTID 180

Query: 181 ARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSDGDGISI 240
           ARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSDGDGISI
Sbjct: 181 ARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSDGDGISI 240

Query: 241 FGSSHIWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKEDEIMQI 300
           FGSSH+WIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKEDEIMQI
Sbjct: 241 FGSSHVWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKEDEIMQI 300

Query: 301 TLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPQNIN 360
           TLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPQNIN
Sbjct: 301 TLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPQNIN 360

Query: 361 AKTITKREYADESEWKKWSWRSEGDLLLNGAVFVGSGDQTKGKKKDLSRYDMISFKPGSY 420
           AKTITKREYADESEWKKWSWRSEGDLL+NGAVFVGSGDQTKGKKKDLSRYDMIS+KPGSY
Sbjct: 361 AKTITKREYADESEWKKWSWRSEGDLLMNGAVFVGSGDQTKGKKKDLSRYDMISYKPGSY 420

Query: 421 VKRLVRFAGTLECRVGKPC 439
           VKRLVRFAGTLECRVGKPC
Sbjct: 421 VKRLVRFAGTLECRVGKPC 436

BLAST of Csor.00g208490 vs. ExPASy TrEMBL
Match: A0A1S3C3Z5 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103496402 PE=3 SV=1)

HSP 1 Score: 767 bits (1980), Expect = 4.33e-278
Identity = 368/439 (83.83%), Postives = 394/439 (89.75%), Query Frame = 0

Query: 1   MAGFRILFVFSLLYFVAAYTDANVDVDDHDEYWLTRANEAKKVAQDAYNPKPESVTEHLN 60
           MAGF  LFV SLL+FV A  +ANVD    DEYW TRA EAKKVA+ A+NP PE VT++LN
Sbjct: 1   MAGFSTLFVCSLLFFVVA--NANVD---EDEYWNTRATEAKKVAKSAFNPNPEIVTDNLN 60

Query: 61  VAVRLAMVGDNSTRRSLNKYKGPCLKTNPIDRCWRCDKNWANNRKKLAECPLGFGHQTIG 120
            AV  AMVG NSTRRSL KY+GPCL TNPIDRCWRCD+NWA NRKKLAECPLGFGHQTIG
Sbjct: 61  AAVSRAMVGRNSTRRSLKKYRGPCLATNPIDRCWRCDRNWAKNRKKLAECPLGFGHQTIG 120

Query: 121 GKRGRIYVVTDASDDNLVDPKPGTLRHGVIQKGPLWIIFGRSMVIRLAQELMISSDKTID 180
           GKRG IYVVTDASDD+LV+PKPGTLR+GVIQKGPLWIIFGRSM+IRL+QELMISSDKTID
Sbjct: 121 GKRGPIYVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTID 180

Query: 181 ARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSDGDGISI 240
           ARG NVHIA+GAGLTIQF +NVIIH LHIH+IISKSGG IRDSVDHYGFRTQSDGDGISI
Sbjct: 181 ARGANVHIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFRTQSDGDGISI 240

Query: 241 FGSSHIWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKEDEIMQI 300
           FGSSH+WIDHVSM  C+DGLIDAIMGST ITISN HFTHHNE MLFGASDSY EDEIMQ+
Sbjct: 241 FGSSHVWIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQV 300

Query: 301 TLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPQNIN 360
           TLAFNHFGRGLVQRMPRCR+GFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAP N N
Sbjct: 301 TLAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPN 360

Query: 361 AKTITKREYADESEWKKWSWRSEGDLLLNGAVFVGSGDQTKGKKKDLSRYDMISFKPGSY 420
           AK +TKREYA E EWK WSWRSEGD L+NGAVF+ SGDQTKGKKK LSRYDMISFKPG+Y
Sbjct: 361 AKQVTKREYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQTKGKKKGLSRYDMISFKPGTY 420

Query: 421 VKRLVRFAGTLECRVGKPC 439
           V+RL RF+GTLEC  GKPC
Sbjct: 421 VRRLARFSGTLECTPGKPC 434

BLAST of Csor.00g208490 vs. ExPASy TrEMBL
Match: A0A6J1GIY2 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111454644 PE=3 SV=1)

HSP 1 Score: 748 bits (1931), Expect = 2.87e-270
Identity = 355/457 (77.68%), Postives = 387/457 (84.68%), Query Frame = 0

Query: 1   MAGFRILFVFSLLYFVAAYTDA------------------NVDVDDHDEYWLTRANEAKK 60
           M  FR LF+ SLL FV  Y+DA                   +D++  DEYW  RA EA+K
Sbjct: 1   MIPFRALFLCSLL-FVVVYSDAPPDGDKTPDVEVDKINDKGIDLEKADEYWQKRAAEAQK 60

Query: 61  VAQDAYNPKPESVTEHLNVAVRLAMVGDNSTRRSLNKYKGPCLKTNPIDRCWRCDKNWAN 120
            AQ A+NP P+SV ++ N AV   +V  N++RRSL +Y+GPCL TNPIDRCWRCD NW  
Sbjct: 61  AAQKAFNPDPDSVADNFNAAVGQEIVNSNNSRRSLRRYRGPCLATNPIDRCWRCDPNWDK 120

Query: 121 NRKKLAECPLGFGHQTIGGKRGRIYVVTDASDDNLVDPKPGTLRHGVIQKGPLWIIFGRS 180
           NRKKLAECPLGFGHQTIGGKRG IYVVTDASD+NLVDPKPGTLRH V+Q GP WIIFGRS
Sbjct: 121 NRKKLAECPLGFGHQTIGGKRGPIYVVTDASDENLVDPKPGTLRHAVVQNGPRWIIFGRS 180

Query: 181 MVIRLAQELMISSDKTIDARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRD 240
           M+IRL+QEL+ISS+KTID RGFNVHIA+GAGLT+QF HN+IIHGLHIH+IISKSGG IRD
Sbjct: 181 MIIRLSQELIISSNKTIDGRGFNVHIAFGAGLTLQFVHNIIIHGLHIHDIISKSGGMIRD 240

Query: 241 SVDHYGFRTQSDGDGISIFGSSHIWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNE 300
           + DHYGFRTQSDGDGISIFG SHIWIDHVSMY CQDGLIDAIMGST ITISNSHFTHHNE
Sbjct: 241 AFDHYGFRTQSDGDGISIFGGSHIWIDHVSMYNCQDGLIDAIMGSTAITISNSHFTHHNE 300

Query: 301 AMLFGASDSYKEDEIMQITLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQ 360
            MLFGASDSYKED IMQITLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQ
Sbjct: 301 VMLFGASDSYKEDSIMQITLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQ 360

Query: 361 HPTIVSQGNRFIAPQNINAKTITKREYADESEWKKWSWRSEGDLLLNGAVFVGSGDQTKG 420
           HPTIVSQGNRFIAP N +AK ITKREYADESEWK WSWRSEGDL++NGA FV +GDQTKG
Sbjct: 361 HPTIVSQGNRFIAPPNPSAKQITKREYADESEWKNWSWRSEGDLMINGAYFVPAGDQTKG 420

Query: 421 KKKDLSRYDMISFKPGSYVKRLVRFAGTLECRVGKPC 439
           KK D SRYDMI FKPG+YVKRL RFAGTLEC+VGKPC
Sbjct: 421 KKSDFSRYDMIKFKPGAYVKRLTRFAGTLECQVGKPC 456

BLAST of Csor.00g208490 vs. ExPASy TrEMBL
Match: A0A5A7UQS9 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00930 PE=3 SV=1)

HSP 1 Score: 698 bits (1801), Expect = 8.11e-252
Identity = 326/373 (87.40%), Postives = 346/373 (92.76%), Query Frame = 0

Query: 67  MVGDNSTRRSLNKYKGPCLKTNPIDRCWRCDKNWANNRKKLAECPLGFGHQTIGGKRGRI 126
           MVG NSTRRSL KY+GPCL TNPIDRCWRCD+NWA NRKKLAECPLGFGHQTIGGKRG I
Sbjct: 1   MVGRNSTRRSLKKYRGPCLATNPIDRCWRCDRNWAKNRKKLAECPLGFGHQTIGGKRGPI 60

Query: 127 YVVTDASDDNLVDPKPGTLRHGVIQKGPLWIIFGRSMVIRLAQELMISSDKTIDARGFNV 186
           YVVTDASDD+LV+PKPGTLR+GVIQKGPLWIIFGRSM+IRL+QELMISSDKTIDARG NV
Sbjct: 61  YVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTIDARGANV 120

Query: 187 HIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSDGDGISIFGSSHI 246
           HIA+GAGLTIQF +NVIIH LHIH+IISKSGG IRDSVDHYGFRTQSDGDGISIFGSSH+
Sbjct: 121 HIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFRTQSDGDGISIFGSSHV 180

Query: 247 WIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKEDEIMQITLAFNH 306
           WIDHVSM  C+DGLIDAIMGST ITISN HFTHHNE MLFGASDSY EDEIMQ+TLAFNH
Sbjct: 181 WIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQVTLAFNH 240

Query: 307 FGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPQNINAKTITK 366
           FGRGLVQRMPRCR+GFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAP N NAK +TK
Sbjct: 241 FGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKQVTK 300

Query: 367 REYADESEWKKWSWRSEGDLLLNGAVFVGSGDQTKGKKKDLSRYDMISFKPGSYVKRLVR 426
           REYA E EWK WSWRSEGD L+NGAVF+ SGDQTKGKKK LSRYDMISFKPG+YV+RL R
Sbjct: 301 REYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQTKGKKKGLSRYDMISFKPGTYVRRLAR 360

Query: 427 FAGTLECRVGKPC 439
           F+GTLEC  GKPC
Sbjct: 361 FSGTLECTPGKPC 373

BLAST of Csor.00g208490 vs. TAIR 10
Match: AT3G01270.1 (Pectate lyase family protein )

HSP 1 Score: 534.6 bits (1376), Expect = 7.2e-152
Identity = 258/455 (56.70%), Postives = 322/455 (70.77%), Query Frame = 0

Query: 22  ANVDVDDHDEYWLTRANEAKKVAQDAYNPKPESVTEHLNV-------------------- 81
           ANV  D+ DEYW+ +ANEA+K    AY+P P  + +H +                     
Sbjct: 24  ANV-ADETDEYWVNKANEARKHTLMAYHPDPYEIVDHFHERHYDNSTDVEGTEEEKAVAS 83

Query: 82  ----AVRLAMVGDNSTRRSL------------NKYKGPCLKTNPIDRCWRCDKNWANNRK 141
                + +     NSTRRSL            +K  GPC  +NPID+CWRC  +WA  RK
Sbjct: 84  EEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTGPCTASNPIDKCWRCQPDWARRRK 143

Query: 142 KLAECPLGFGHQTIGGKRGRIYVVTDASDDNLVDPKPGTLRHGVIQKGPLWIIFGRSMVI 201
           KL  C  GFG++T GGKRGRIYVVT   DD++V+P+PGTLRH VIQK PLWI+F   M I
Sbjct: 144 KLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTLRHAVIQKEPLWIVFKHDMSI 203

Query: 202 RLAQELMISSDKTIDARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVD 261
           RL+QELMI+SDKTIDARG NVHIAYGAG+T+Q+ HN+IIHGLH+H+I+  SGG IRDS++
Sbjct: 204 RLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNIIIHGLHVHHIVKSSGGLIRDSIN 263

Query: 262 HYGFRTQSDGDGISIFGSSHIWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAML 321
           H+G R ++DGDGISIFG+++IW+DH+SM  CQDGLIDAIMGST ITISNSHFTHHN+ ML
Sbjct: 264 HFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLIDAIMGSTAITISNSHFTHHNDVML 323

Query: 322 FGASDSYKEDEIMQITLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPT 381
            GA ++  +D+ MQ+T+A+NHFG+GLVQRMPR R+GF HVVNNDYTHW +YAIGGSQ PT
Sbjct: 324 LGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHVVNNDYTHWELYAIGGSQGPT 383

Query: 382 IVSQGNRFIA-PQNINAKTITKREYADESEWKKWSWRSEGDLLLNGAVFVGSGDQTKGKK 440
           I+S GNRFIA P   + + +TKR+YA ESEWK W+WRSE D+ +N A F  SG+     K
Sbjct: 384 ILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWRSEKDVFMNNAYFRQSGN--PHFK 443

BLAST of Csor.00g208490 vs. TAIR 10
Match: AT1G14420.1 (Pectate lyase family protein )

HSP 1 Score: 523.9 bits (1348), Expect = 1.3e-148
Identity = 261/454 (57.49%), Postives = 316/454 (69.60%), Query Frame = 0

Query: 1   MAGFRILFVFSLLYFVAAYTDANVDVDDHDEYWLTRANEAKKVAQDAYNPKPESVTEHLN 60
           + G+  +F  S L  VA     NV V   D YW  R ++A K    +Y+P P +VT H N
Sbjct: 8   LGGYVFVFFSSFLAIVAPQVRGNVAV--FDSYWTQRQSDALKQTIGSYDPHPLNVTNHFN 67

Query: 61  VAVRLAMVGD---NSTRRSL---------NKYKGPCLKTNPIDRCWRCDKNWANNRKKLA 120
             V +A+      N TRR L         +K  G CL  NPID CWRCD+NWANNRKKLA
Sbjct: 68  YHVNIAVDASESRNDTRRELTQVRSGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLA 127

Query: 121 ECPLGFGHQTIGGKRGRIYVVTDASDDNLVDPKPGTLRHGVIQKGPLWIIFGRSMVIRLA 180
           +C LGFG +T GGK G IYVV DASD++L++PKPGTLRH V + GPLWIIF RSM+I+L 
Sbjct: 128 DCVLGFGRRTTGGKDGPIYVVKDASDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQ 187

Query: 181 QELMISSDKTIDARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYG 240
           QELMI+SDKTID RG  V+I  GAGLT+QF +NVIIH +++ +I+  +GG IRDS  H G
Sbjct: 188 QELMITSDKTIDGRGARVYIMEGAGLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIG 247

Query: 241 FRTQSDGDGISIFGSSHIWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGA 300
            RT+SDGDGIS+FG+++IWIDHVSM  C DG+IDAI GST +TISNSHFT H E MLFGA
Sbjct: 248 LRTKSDGDGISLFGATNIWIDHVSMTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGA 307

Query: 301 SDSYKEDEIMQITLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVS 360
            D +  D+ MQIT+AFNHFG+ L QRMPRCR+G  HVVNNDYTHW MYAIGG+ +PTI+S
Sbjct: 308 RDEHVIDKKMQITVAFNHFGKRLEQRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIIS 367

Query: 361 QGNRFIAPQNINAKTITKREYADESEWKKWSWRSEGDLLLNGAVFVGSGDQ---TKGKKK 420
           QGNRFIAP N  AK ITKREY    EWK W+W+SEGD  LNGA FV SG     +   K 
Sbjct: 368 QGNRFIAPPNEEAKQITKREYTPYGEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKT 427

Query: 421 DLSRYDMISFKPGSYVKRLVRFAGTLECRVGKPC 440
            L     I  KPG+ V++L   AG L C++G+ C
Sbjct: 428 PLPNKFTIRPKPGTMVRKLTMDAGVLGCKLGEAC 459

BLAST of Csor.00g208490 vs. TAIR 10
Match: AT5G15110.1 (Pectate lyase family protein )

HSP 1 Score: 521.9 bits (1343), Expect = 4.9e-148
Identity = 254/470 (54.04%), Postives = 324/470 (68.94%), Query Frame = 0

Query: 5   RILFVFSLLYFVAAYTDANVDVDDHDEYWLTRANEAKKVAQDAYNPKPESVTEHLNVA-- 64
           +++F F +   +        ++ + DEYW  RA+EA++    AY+  P  + +H +    
Sbjct: 8   KLMFTFCIAVLIPTIRG---NISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHERHY 67

Query: 65  ------------------------VRLAMVGDNSTRRSL--------NKYKGPCLKTNPI 124
                                   + +     NSTRRSL        +K KGPC  +NPI
Sbjct: 68  DNSTDVTTPEEDGDAKPEEEEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASNPI 127

Query: 125 DRCWRCDKNWANNRKKLAECPLGFGHQTIGGKRGRIYVVTDASDDNLVDPKPGTLRHGVI 184
           D+CWRC  +WA  RKKL  C  GFGH+T GGKRGRIYVVT   D+++V+PKPGTLRH VI
Sbjct: 128 DKCWRCRSDWAKRRKKLTRCVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHAVI 187

Query: 185 QKGPLWIIFGRSMVIRLAQELMISSDKTIDARGFNVHIAYGAGLTIQFAHNVIIHGLHIH 244
           QK PLWIIF   M IRL QEL+I+S KTIDARG NVH+A+GAG+T+QF  NV+IHGLHIH
Sbjct: 188 QKEPLWIIFKNDMSIRLNQELLINSHKTIDARGANVHVAHGAGITMQFVKNVVIHGLHIH 247

Query: 245 NIISKSGGYIRDSVDHYGFRTQSDGDGISIFGSSHIWIDHVSMYACQDGLIDAIMGSTGI 304
           +I   SGG IRDSVDH+G RT++DGDG+SI+GSS+IW+DH+SM  CQDGLIDAI+GSTGI
Sbjct: 248 HISESSGGMIRDSVDHFGMRTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGSTGI 307

Query: 305 TISNSHFTHHNEAMLFGASDSYKEDEIMQITLAFNHFGRGLVQRMPRCRFGFFHVVNNDY 364
           TISNSHFTHHN+ ML GA ++ + D+ MQ+T+A+NHFG+GLVQRMPR R+GF HVVNNDY
Sbjct: 308 TISNSHFTHHNDVMLLGAQNTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNNDY 367

Query: 365 THWLMYAIGGSQHPTIVSQGNRFIA-PQNINAKTITKREYADESEWKKWSWRSEGDLLLN 424
           THW +YAIGGSQ PTI+S GNRFIA P   + + +TKR+YA E EWK W+WRS+ D+ +N
Sbjct: 368 THWELYAIGGSQGPTILSHGNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVFMN 427

Query: 425 GAVFVGSGDQTKGKKKDLSRYDMISFKPGSYVKRLVRFAGTLECRVGKPC 440
           GA F  SG+     K   +R  MI  K G  V +L ++AG L+CRVG+ C
Sbjct: 428 GAYFRQSGNPQ--YKCAHTRQQMIKPKNGLAVSKLTKYAGALDCRVGRRC 472

BLAST of Csor.00g208490 vs. TAIR 10
Match: AT2G02720.1 (Pectate lyase family protein )

HSP 1 Score: 491.5 bits (1264), Expect = 7.0e-139
Identity = 245/448 (54.69%), Postives = 302/448 (67.41%), Query Frame = 0

Query: 7   LFVFSLLYFVAAYTDANVDVDDHDEYWLTRANEAKKVAQDAYNPKPESVTEHLNVAVRLA 66
           +FVF  L            + ++DEYW  R   A +   ++Y+P PE+VT+H N    LA
Sbjct: 8   VFVFVALSLTVVVPSVQAHIAEYDEYWTQRQTNALRETLESYDPNPENVTDHFNYHAALA 67

Query: 67  MVGD---NSTRRSL---------NKYKGPCLKTNPIDRCWRCDKNWANNRKKLAECPLGF 126
           M      N TRR L          +  G     N ID+CWR DKNW  NRKKLA+C LGF
Sbjct: 68  METTGIVNETRRDLRQVGRGKKTTRRGGRFESLNAIDKCWRGDKNWDKNRKKLADCVLGF 127

Query: 127 GHQTIGGKRGRIYVVTDASDDNLVDPKPGTLRHGVIQKGPLWIIFGRSMVIRLAQELMIS 186
           G +T GGK G IYVVTD SD++L+ PKPGT+RH V +  PLWIIF RSM+I+L QEL+I+
Sbjct: 128 GRKTTGGKNGPIYVVTDPSDNDLLKPKPGTIRHAVTRDRPLWIIFARSMIIKLQQELIIT 187

Query: 187 SDKTIDARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSD 246
           +DKTID RG  ++I  GAGLT+QF  NVIIH +HI  I   +GG I DS  H+G RT SD
Sbjct: 188 NDKTIDGRGAKIYITGGAGLTLQFVRNVIIHNIHIKQIKRGAGGLIIDSEQHFGLRTVSD 247

Query: 247 GDGISIFGSSHIWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKE 306
           GDGI+IFG++++WIDHVSM  C DG+IDAIMGST ITISNSHFT H+E MLFG ++    
Sbjct: 248 GDGINIFGATNVWIDHVSMTDCSDGMIDAIMGSTAITISNSHFTDHDEVMLFGGTNKDVI 307

Query: 307 DEIMQITLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFI 366
           D+ MQIT+AFNHFG+ L QRMPR RFG  HVVNNDYTHW MYAIGG+ +PTI+SQGNRFI
Sbjct: 308 DKKMQITVAFNHFGKRLKQRMPRVRFGLVHVVNNDYTHWEMYAIGGNMNPTIISQGNRFI 367

Query: 367 APQNINAKTITKREYADESEWKKWSWRSEGDLLLNGAVFVGSGDQ---TKGKKKDLSRYD 426
           AP   ++K +TKREY    EWK W+W+SE D  LNGA FV SG     +   K  + R  
Sbjct: 368 APPIEDSKQVTKREYTPYPEWKSWNWQSEKDYFLNGAYFVQSGKANAWSATPKNPIPRKF 427

Query: 427 MISFKPGSYVKRLVRFAGTLECRVGKPC 440
            I  +PG+ V+RL + AGTL C+ GK C
Sbjct: 428 AIRPQPGTKVRRLTKDAGTLGCKPGKSC 455

BLAST of Csor.00g208490 vs. TAIR 10
Match: AT4G13710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 489.6 bits (1259), Expect = 2.7e-138
Identity = 234/405 (57.78%), Postives = 288/405 (71.11%), Query Frame = 0

Query: 35  TRANEAKKVAQDAYNPKPESVTEHLNVAVRLAMVGDNSTRRSLNKYKGPCLKTNPIDRCW 94
           TR +   +  Q A    P+ V + +   V+++   + + RR L  +   C   NPID CW
Sbjct: 72  TRLDGEPQQQQHAVADDPDMVADEVAKLVQMS-EQNRTARRKLGFFS--CGTGNPIDDCW 131

Query: 95  RCDKNWANNRKKLAECPLGFGHQTIGGKRGRIYVVTDASDDNLVDPKPGTLRHGVIQKGP 154
           RCD+NW  NRK+LA+C +GFG   IGG+ GR Y+VTD +D+++V+PKPGTLRH VIQ+ P
Sbjct: 132 RCDRNWHKNRKRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHAVIQEEP 191

Query: 155 LWIIFGRSMVIRLAQELMISSDKTIDARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIIS 214
           LWI+F R MVI L QEL+++S KTIDARG NVHIA GA +TIQF  NVIIHGLHIH+   
Sbjct: 192 LWIVFKRDMVIELKQELIMNSFKTIDARGSNVHIANGACITIQFITNVIIHGLHIHDCKP 251

Query: 215 KSGGYIRDSVDHYGFRTQSDGDGISIFGSSHIWIDHVSMYACQDGLIDAIMGSTGITISN 274
                +R S  H+G+RT +DGD +SIFGSSHIWIDH S+  C DGL+DA+MGST IT+SN
Sbjct: 252 TGNAMVRSSPSHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGSTAITVSN 311

Query: 275 SHFTHHNEAMLFGASDSYKEDEIMQITLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWL 334
           +HFTHHNE ML G SDSY +D++MQ+T+A+NHFG GLVQRMPRCR G+FHVVNNDYTHW 
Sbjct: 312 NHFTHHNEVMLLGHSDSYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWE 371

Query: 335 MYAIGGSQHPTIVSQGNRFIAPQNINAKTITKREYADESEWKKWSWRSEGDLLLNGAVFV 394
           MYAIGGS  PTI SQGNR+ AP +  AK +TKR   D SEWKKW+WRSEGDLLLNGA F 
Sbjct: 372 MYAIGGSAEPTINSQGNRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFR 431

Query: 395 GSGDQTKGKKKDLSRYDMISFKPGSYVKRLVRFAGTLECRVGKPC 440
            SG    G      R   ++ KP S V  +   AG L CR G+PC
Sbjct: 432 PSG---AGASASYGRASSLAAKPSSMVDTITSTAGALGCRKGRPC 470

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P157221.6e-15962.30Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1[more]
P409732.7e-15962.36Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1[more]
Q9SRH41.0e-15056.70Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1[more]
Q9M9S21.8e-14757.49Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1[more]
Q9LFP55.2e-14754.26Probable pectate lyase 19 OS=Arabidopsis thaliana OX=3702 GN=At5g15110 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022946578.10.0100.00pectate lyase-like [Cucurbita moschata] >KAG6599067.1 putative pectate lyase P59... [more]
XP_023523501.10.097.27pectate lyase-like [Cucurbita pepo subsp. pepo][more]
XP_022999690.10.097.04pectate lyase-like [Cucurbita maxima][more]
KAG7030004.10.097.49putative pectate lyase P59 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_008456459.18.94e-27883.83PREDICTED: pectate lyase-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A6J1G4950.0100.00Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111450600 PE=3 SV=1[more]
A0A6J1KG970.097.04Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111493968 PE=3 SV=1[more]
A0A1S3C3Z54.33e-27883.83Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103496402 PE=3 SV=1[more]
A0A6J1GIY22.87e-27077.68Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111454644 PE=3 SV=1[more]
A0A5A7UQS98.11e-25287.40Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00930 ... [more]
Match NameE-valueIdentityDescription
AT3G01270.17.2e-15256.70Pectate lyase family protein [more]
AT1G14420.11.3e-14857.49Pectate lyase family protein [more]
AT5G15110.14.9e-14854.04Pectate lyase family protein [more]
AT2G02720.17.0e-13954.69Pectate lyase family protein [more]
AT4G13710.12.7e-13857.78Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018082AmbAllergenPRINTSPR00807AMBALLERGENcoord: 234..255
score: 63.42
coord: 314..333
score: 79.05
coord: 110..127
score: 58.47
coord: 410..433
score: 38.49
coord: 134..159
score: 66.48
coord: 170..186
score: 63.31
coord: 336..355
score: 77.86
coord: 378..402
score: 51.24
IPR002022Pectate lyaseSMARTSM00656amb_allcoord: 162..359
e-value: 7.5E-99
score: 344.4
IPR002022Pectate lyasePFAMPF00544Pectate_lyase_4coord: 194..353
e-value: 5.7E-18
score: 65.3
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 87..432
e-value: 1.0E-110
score: 371.9
IPR007524Pectate lyase, N-terminalPFAMPF04431Pec_lyase_Ncoord: 27..77
e-value: 2.9E-17
score: 62.7
IPR045032Pectin lyase familyPANTHERPTHR31683PECTATE LYASE 18-RELATEDcoord: 5..439
NoneNo IPR availablePANTHERPTHR31683:SF132PECTATE LYASEcoord: 5..439
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 78..433

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g208490.m01Csor.00g208490.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0046872 metal ion binding
molecular_function GO:0030570 pectate lyase activity