Csor.00g208370 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g208370
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionTerpene cyclase/mutase family member
LocationCsor_Chr05: 7761658 .. 7764159 (-)
RNA-Seq ExpressionCsor.00g208370
SyntenyCsor.00g208370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGAGACTTAAGCTGGGGGAAGGAGCAAACGATCCATACTTATTCAGCTCCAATAACTTCGTGGGAAGACAAACATGGGACTTCGAAGTTGATGAAGGCACTCCAAAAGAGCGAGCTGAAGTGGAAGAAGCCTGCCAAAATTTTTATCAGAATCGCTTCAAAGTTCAATGCAGCAGTAACTTTTTTTGGAAATTTCAGGTTTTCTTTAATGATTGCATAACTCATTTAAGTGTTTATCTATTTTAAGTTTTAAACCTTTTTTAATCGTGTTTTACTTTCCTAAGTTTAAATCTAAATGGGAATAACTACCGTGTTTAGTTATTTAATATTTTTTTTCCCGATTATCAAATATAGATTGATAGGTTTATTAAAAATATGAAATTTAATTTATTGTACCTATATCATCCTACCCTCTGCATATTCCTTCGGCTGACAAACTGCTTACTCACTACCCATTGCATCAGCGAGAGAGGAGAATGATATAAATTTTTATAAGAATGTGGAAACTTTTTTCTAACAGACGCGTTTTAAAAACTTTGAGGAGAAACCCGGAAGGAAAATCTCAAAGAAAATAATATCTGCTAGCGGTAGACTTGTAGACTTGTATTGTTATAAATGGTATTAGAGGCAAATACGAGACGATGTGCCAGCGAGGAGGCTGAGCCTCATAAGAGGTGGACACGAAGTGGTGTGCTAGCAAGAACGCTGGACCTTGAAGGGGATAGATTGGTGGGAGTCCCACATTGTTTGAAAAAGGGAATGAGTGTCAACGAGGACGCTGACCCCGAAGTAAGGTGGAGTGTGAGATCCTGCATTCGTTGGAACAAAAATAAAATATTCTTTATAACTGTATGAAAATCTTTTCATAGCATACGCGTTTTAAAAACCTTAAAGAGAAGCTCGAAAAGGAACAATAATTTCATTAAAAGAAACAAATGCGAGTAGGTTGGAAATGAAGTTATAATAAGTTATTATGAGAATGCAGGTTCTAAGAGAGAGAAATTTCAAGCAAACAATTCCAGTAGTGAAAGTGGAGGAAGGAATAGATGGGGACGAGAAAGTAGCATTAATTAATAAGGAAACAGCGAGCATTGCACTGAGAAGAGCCACCAAGTTCTTTGCAGCCTTACAAAGCCACCATGGCCATTGGCCTGCTGAAAACTCTGGCCCTATGTTTTACTTCCCTCCACTGGTACTCTTCCTTTTTCTATTCATTTTATCTAATATAATTCTATCACAATATTACCCTTTTCTTTTCAGGTATTTTCGTTGTATATTACGGGACATCTCGGCATTATATTCTCAGAAGAGCACCGAAGGGAAATCCTTCGCTATGCATATTGTCATCAGAATGAAGATGGTGGATGGGGATTGCACATTGAGGGACAAAGTTGTATGCTTTGCACTGTTTTTAACTATATTCAACTCCGTTTGTTGGGGGAAGAACCTGACAAGAATGAGTGTTTTAGAGCTCGAAAATGGATTCTAGATCATGGAGGTGCTATTTATATACCTTCCTGGGGAAAGATTTGGCTATCGGTTAGTATTGTATATATACGTCTATACTATTTTATTTGAAACAGTGTAACTCAAATTTAACGAACTAAACTTATAATTTAACTTTGTAGATTTTGGGAGTTTACGAGTGGGAGGGAGCAAACCCTATGCCTCCAGAGTTTTGGCTGTTAGGAAAATTACTTCCTTTCATTCCTCAAAGTTTGTTTTGCTATTCGAGATTGACACTTCTTCCCATGTCGTATTTATTTGGGAAGCGATTTGTTGGACCTCTCACCCCTCTCATTCTTCAATTACGCCAAGAAATCTATACTCAACCTTACAATCACATTAAATGGAGTCCAACACGCCATTATTGTGCAAAGGTACGTTCCTAAATCATTCAATCCTCCCTACAAAAATGCACCAGAAAAAGATTATAGAATTTCTACGCACAAATCAATATTCATCCTTTCAAAAGGTTTGAGATTTCTATGTGTAGAAATTGATCGTTTCCATTTTAGGTACGGTATGAGACTTTGATTATTTTGATTGAATTAGATATGATGATGCAGGAAGACAAATGCTTTGAACGTTCATTATTTCAGAAGCTAACATGGGATGCTATTCAATACTTTGGAGAACCCATTCTTAATAGTTGGGCCTTTAAAACGATAAGAAATAGTGCTCTTCAAGTAGCTAAATGTCATATTGATTATGAAGATCATAACAGTCATTACATTACAATTGGATGCGTTGAAAAGGTTAATTTCGTACTTCTTTATTCTTTTATTATTATCATTATAGTTAATAACATTACATTAATAAATATAATTGAGCTAATATGGTGGAGGGGTATTGAACATGCAGCCATTGTTCACACTTGCTTGTTGGGTCGATGATCCTAATGGGGAAGCTTACAAGAAACATGTTGCTAGAATCAAAGATTACTTATGGATTGCTGAAGATGGAATGAAGATGCAAGTAAGTATTACCCATTATTAA

mRNA sequence

ATGTGGAGACTTAAGCTGGGGGAAGGAGCAAACGATCCATACTTATTCAGCTCCAATAACTTCGTGGGAAGACAAACATGGGACTTCGAAGTTGATGAAGGCACTCCAAAAGAGCGAGCTGAAGTGGAAGAAGCCTGCCAAAATTTTTATCAGAATCGCTTCAAAGTTCAATGCAGCAGTAACTTTTTTTGGAAATTTCAGGTTCTAAGAGAGAGAAATTTCAAGCAAACAATTCCAGTAGTGAAAGTGGAGGAAGGAATAGATGGGGACGAGAAAGTAGCATTAATTAATAAGGAAACAGCGAGCATTGCACTGAGAAGAGCCACCAAGTTCTTTGCAGCCTTACAAAGCCACCATGGCCATTGGCCTGCTGAAAACTCTGGCCCTATGTTTTACTTCCCTCCACTGGTATTTTCGTTGTATATTACGGGACATCTCGGCATTATATTCTCAGAAGAGCACCGAAGGGAAATCCTTCGCTATGCATATTGTCATCAGAATGAAGATGGTGGATGGGGATTGCACATTGAGGGACAAAGTTGTATGCTTTGCACTGTTTTTAACTATATTCAACTCCGTTTGTTGGGGGAAGAACCTGACAAGAATGAGTGTTTTAGAGCTCGAAAATGGATTCTAGATCATGGAGGTGCTATTTATATACCTTCCTGGGGAAAGATTTGGCTATCGATTTTGGGAGTTTACGAGTGGGAGGGAGCAAACCCTATGCCTCCAGAGTTTTGGCTGTTAGGAAAATTACTTCCTTTCATTCCTCAAAGTTTGTTTTGCTATTCGAGATTGACACTTCTTCCCATGTCGTATTTATTTGGGAAGCGATTTGTTGGACCTCTCACCCCTCTCATTCTTCAATTACGCCAAGAAATCTATACTCAACCTTACAATCACATTAAATGGAGTCCAACACGCCATTATTGTGCAAAGGAAGACAAATGCTTTGAACGTTCATTATTTCAGAAGCTAACATGGGATGCTATTCAATACTTTGGAGAACCCATTCTTAATAGTTGGGCCTTTAAAACGATAAGAAATAGTGCTCTTCAAGTAGCTAAATGTCATATTGATTATGAAGATCATAACAGTCATTACATTACAATTGGATGCGTTGAAAAGCCATTGTTCACACTTGCTTGTTGGGTCGATGATCCTAATGGGGAAGCTTACAAGAAACATGTTGCTAGAATCAAAGATTACTTATGGATTGCTGAAGATGGAATGAAGATGCAAGTAAGTATTACCCATTATTAA

Coding sequence (CDS)

ATGTGGAGACTTAAGCTGGGGGAAGGAGCAAACGATCCATACTTATTCAGCTCCAATAACTTCGTGGGAAGACAAACATGGGACTTCGAAGTTGATGAAGGCACTCCAAAAGAGCGAGCTGAAGTGGAAGAAGCCTGCCAAAATTTTTATCAGAATCGCTTCAAAGTTCAATGCAGCAGTAACTTTTTTTGGAAATTTCAGGTTCTAAGAGAGAGAAATTTCAAGCAAACAATTCCAGTAGTGAAAGTGGAGGAAGGAATAGATGGGGACGAGAAAGTAGCATTAATTAATAAGGAAACAGCGAGCATTGCACTGAGAAGAGCCACCAAGTTCTTTGCAGCCTTACAAAGCCACCATGGCCATTGGCCTGCTGAAAACTCTGGCCCTATGTTTTACTTCCCTCCACTGGTATTTTCGTTGTATATTACGGGACATCTCGGCATTATATTCTCAGAAGAGCACCGAAGGGAAATCCTTCGCTATGCATATTGTCATCAGAATGAAGATGGTGGATGGGGATTGCACATTGAGGGACAAAGTTGTATGCTTTGCACTGTTTTTAACTATATTCAACTCCGTTTGTTGGGGGAAGAACCTGACAAGAATGAGTGTTTTAGAGCTCGAAAATGGATTCTAGATCATGGAGGTGCTATTTATATACCTTCCTGGGGAAAGATTTGGCTATCGATTTTGGGAGTTTACGAGTGGGAGGGAGCAAACCCTATGCCTCCAGAGTTTTGGCTGTTAGGAAAATTACTTCCTTTCATTCCTCAAAGTTTGTTTTGCTATTCGAGATTGACACTTCTTCCCATGTCGTATTTATTTGGGAAGCGATTTGTTGGACCTCTCACCCCTCTCATTCTTCAATTACGCCAAGAAATCTATACTCAACCTTACAATCACATTAAATGGAGTCCAACACGCCATTATTGTGCAAAGGAAGACAAATGCTTTGAACGTTCATTATTTCAGAAGCTAACATGGGATGCTATTCAATACTTTGGAGAACCCATTCTTAATAGTTGGGCCTTTAAAACGATAAGAAATAGTGCTCTTCAAGTAGCTAAATGTCATATTGATTATGAAGATCATAACAGTCATTACATTACAATTGGATGCGTTGAAAAGCCATTGTTCACACTTGCTTGTTGGGTCGATGATCCTAATGGGGAAGCTTACAAGAAACATGTTGCTAGAATCAAAGATTACTTATGGATTGCTGAAGATGGAATGAAGATGCAAGTAAGTATTACCCATTATTAA

Protein sequence

MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKERAEVEEACQNFYQNRFKVQCSSNFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALINKETASIALRRATKFFAALQSHHGHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRREILRYAYCHQNEDGGWGLHIEGQSCMLCTVFNYIQLRLLGEEPDKNECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGANPMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQPYNHIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSWAFKTIRNSALQVAKCHIDYEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHVARIKDYLWIAEDGMKMQVSITHY
Homology
BLAST of Csor.00g208370 vs. ExPASy Swiss-Prot
Match: Q2XPU7 (Lupeol synthase OS=Ricinus communis OX=3988 PE=1 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 4.1e-165
Identity = 259/417 (62.11%), Postives = 316/417 (75.78%), Query Frame = 0

Query: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKERAEVEEACQNFYQNRFKVQCSS 60
           MWR+K+ EG N+PY++S+NNF GRQ W F+ + GTP+E+AEVEEA QNF++NRF+V+ +S
Sbjct: 1   MWRIKIAEGGNNPYIYSTNNFQGRQIWVFDPNAGTPEEQAEVEEARQNFWKNRFQVKPNS 60

Query: 61  NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALINKETASIALRRATKFFAALQSHHG 120
           +  W+ Q LRE+NFKQ IP VKVE   DG+E    I  E A+ ALRR+   F+ALQ+  G
Sbjct: 61  DLLWQLQFLREKNFKQKIPKVKVE---DGEE----ITSEIAAAALRRSVHLFSALQASDG 120

Query: 121 HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRREILRYAYCHQNEDGGWGLHIEGQS 180
           HW AEN G +F+ PPLVF++YITGHL  +FS EHR+EILRY YCHQNEDGGWG+HIEG S
Sbjct: 121 HWCAENGGLLFFLPPLVFAVYITGHLNTVFSPEHRKEILRYIYCHQNEDGGWGIHIEGHS 180

Query: 181 CMLCTVFNYIQLRLLGEEPD---KNECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWE 240
            M CTV NYI +R+LGE  D   +N C R RKWILDHGGA  I SWGK WLSILGVYEW+
Sbjct: 181 TMFCTVLNYICMRILGEARDGGIENACERGRKWILDHGGATGISSWGKTWLSILGVYEWD 240

Query: 241 GANPMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQ 300
           G NPMPPEFW      P  P  +FCY R+T +PMSYL+GKRFVGP+TPLILQ+R+EIY +
Sbjct: 241 GTNPMPPEFWAFPSSFPLHPAKMFCYCRITYMPMSYLYGKRFVGPITPLILQIREEIYNE 300

Query: 301 PYNHIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSWAFKTIRNSALQVAKC 360
           PYN IKW+  RH CAKED  F     QKL WDA+  F EP+ + W F  +R  AL++   
Sbjct: 301 PYNKIKWNSVRHLCAKEDNYFPHPTIQKLLWDALYTFSEPLFSRWPFNKLREKALKITMD 360

Query: 361 HIDYEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHVARIKDYLWIAEDGMKMQ 415
           HI YEDHNS YITIGCVEKPL  LACW++DP+GEA+KKH+ARI DY+W+ EDG+KMQ
Sbjct: 361 HIHYEDHNSRYITIGCVEKPLCMLACWIEDPHGEAFKKHLARIADYIWVGEDGIKMQ 410

BLAST of Csor.00g208370 vs. ExPASy Swiss-Prot
Match: Q8W3Z1 (Beta-amyrin synthase OS=Betula platyphylla OX=78630 GN=OSCBPY PE=1 SV=1)

HSP 1 Score: 577.8 bits (1488), Expect = 1.0e-163
Identity = 256/418 (61.24%), Postives = 318/418 (76.08%), Query Frame = 0

Query: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKERAEVEEACQNFYQNRFKVQCSS 60
           MWRLK+ +G +DPY++S+NNFVGRQTW+F+   G+P+ERAEVEEA +NFY NR++V+ S 
Sbjct: 1   MWRLKIADGGSDPYIYSTNNFVGRQTWEFDPQAGSPQERAEVEEARRNFYDNRYQVKPSG 60

Query: 61  NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALINKETASIALRRATKFFAALQSHHG 120
           +  W+ Q L+E+NFKQTIP VKVE   DG+E    I  E ++ ALRRA  F++ALQ+  G
Sbjct: 61  DLLWRMQFLKEKNFKQTIPPVKVE---DGEE----ITYEKSTAALRRAVHFYSALQASDG 120

Query: 121 HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRREILRYAYCHQNEDGGWGLHIEGQS 180
           HWPAEN+GP+F+ PPLV  +YITGHL  +F  EH++EILRY Y HQNEDGGWGLHIEG S
Sbjct: 121 HWPAENAGPLFFLPPLVMCMYITGHLNTVFPAEHQKEILRYIYYHQNEDGGWGLHIEGHS 180

Query: 181 CMLCTVFNYIQLRLLGEEPD---KNECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWE 240
            M CT  +YI +R+LGE PD    N C RARKWILDHGG  ++PSWGK WLSILG++EW 
Sbjct: 181 TMFCTALSYICMRILGEGPDGGQDNACARARKWILDHGGVTHMPSWGKTWLSILGIFEWI 240

Query: 241 GANPMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQ 300
           G+NPMPPEFW+L   LP  P  ++CY R+  +PMSYL+GKRFVGP+TPLILQLR+E+YTQ
Sbjct: 241 GSNPMPPEFWILPSFLPMHPAKMWCYCRMVYMPMSYLYGKRFVGPITPLILQLREELYTQ 300

Query: 301 PYNHIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSWAF-KTIRNSALQVAK 360
           PY+ + W   RH CAKED  +   L Q L WD++  F EP+L  W F K +R  ALQV  
Sbjct: 301 PYHQVNWKKVRHLCAKEDIYYPHPLIQDLLWDSLYIFTEPLLTRWPFNKLVREKALQVTM 360

Query: 361 CHIDYEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHVARIKDYLWIAEDGMKMQ 415
            HI YED NS YITIGCVEK L  LACWV+DPNG+ +KKH+ARI DY+W+AEDG+KMQ
Sbjct: 361 KHIHYEDENSRYITIGCVEKVLCMLACWVEDPNGDYFKKHIARIPDYIWVAEDGIKMQ 411

BLAST of Csor.00g208370 vs. ExPASy Swiss-Prot
Match: E7DN63 (Beta-amyrin synthase OS=Solanum lycopersicum OX=4081 GN=TTS1 PE=1 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 6.5e-163
Identity = 257/417 (61.63%), Postives = 311/417 (74.58%), Query Frame = 0

Query: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKERAEVEEACQNFYQNRFKVQCSS 60
           MW+LK+ EG N PYL+S+NN+VGRQTW+F+ + GT +ERA++EEA Q F+ NR+KV+ SS
Sbjct: 1   MWKLKIAEGQNGPYLYSTNNYVGRQTWEFDPNGGTIEERAKIEEARQQFWNNRYKVKPSS 60

Query: 61  NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALINKETASIALRRATKFFAALQSHHG 120
           +  W+ Q L E+NFKQ IP VKVEEG +       I+ E A+IAL RA  FF+ALQ+  G
Sbjct: 61  DLLWRIQFLGEKNFKQKIPAVKVEEGEE-------ISHEVATIALHRAVNFFSALQATDG 120

Query: 121 HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRREILRYAYCHQNEDGGWGLHIEGQS 180
           HWPAEN+GP+F+ PPLV  +YITGHL  +F  EHR+EILRY YCHQNEDGGWGLHIEG S
Sbjct: 121 HWPAENAGPLFFLPPLVMCMYITGHLNTVFPAEHRKEILRYIYCHQNEDGGWGLHIEGHS 180

Query: 181 CMLCTVFNYIQLRLLGEEPD---KNECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWE 240
            M CT  +YI +R+LGE PD    N C RARKWILDHG    IPSWGK WLSILGV+EW 
Sbjct: 181 TMFCTALSYICMRILGEGPDGGVNNACARARKWILDHGSVTAIPSWGKTWLSILGVFEWI 240

Query: 241 GANPMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQ 300
           G NPMPPEFW+L   LP  P  ++CY R+  +PMSYL+GKRFVGP+TPLILQLR+E+Y +
Sbjct: 241 GTNPMPPEFWILPSFLPVHPAKMWCYCRMVYMPMSYLYGKRFVGPITPLILQLREELYDR 300

Query: 301 PYNHIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSWAFKTIRNSALQVAKC 360
           PY+ I W   RH CAKED  +   L Q L WD++    EP+L  W F  +RN AL+V   
Sbjct: 301 PYDEINWKKVRHVCAKEDLYYPHPLVQDLMWDSLYICTEPLLTRWPFNKLRNKALEVTMK 360

Query: 361 HIDYEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHVARIKDYLWIAEDGMKMQ 415
           HI YED NS YITIGCVEK L  LACWV+DPNG+ +KKH+ARI DYLW+AEDGMKMQ
Sbjct: 361 HIHYEDENSRYITIGCVEKVLCMLACWVEDPNGDYFKKHLARIPDYLWVAEDGMKMQ 410

BLAST of Csor.00g208370 vs. ExPASy Swiss-Prot
Match: Q9LRH8 (Beta-amyrin synthase OS=Pisum sativum OX=3888 GN=OSCPSY PE=2 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 3.9e-160
Identity = 255/418 (61.00%), Postives = 310/418 (74.16%), Query Frame = 0

Query: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKERAEVEEACQNFYQNRFKVQCSS 60
           MWRLK+ EG NDPYLFS+NNFVGRQTW+++ + G+ +ERA+VEEA +NFY NRF+V+   
Sbjct: 1   MWRLKIAEGGNDPYLFSTNNFVGRQTWEYDPEAGSEEERAQVEEARRNFYNNRFEVKPCG 60

Query: 61  NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALINKETASIALRRATKFFAALQSHHG 120
           +  W+FQVLRE NFKQTI  VK+E   D +E    I  E  +  LRR T   A LQ+  G
Sbjct: 61  DLLWRFQVLRENNFKQTIGGVKIE---DEEE----ITYEKTTTTLRRGTHHLATLQTSDG 120

Query: 121 HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRREILRYAYCHQNEDGGWGLHIEGQS 180
           HWPA+ +GP+F+ PPLVF +YITGHL  +F  EHR+EILRY YCHQNEDGGWGLHIEG S
Sbjct: 121 HWPAQIAGPLFFMPPLVFCVYITGHLDSVFPPEHRKEILRYIYCHQNEDGGWGLHIEGHS 180

Query: 181 CMLCTVFNYIQLRLLGEEPD---KNECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWE 240
            M CT  NYI +R+LGE PD    N C RAR WI  HGG  +IPSWGK WLSILGV++W 
Sbjct: 181 TMFCTALNYICMRILGEGPDGGEDNACVRARNWIRQHGGVTHIPSWGKTWLSILGVFDWL 240

Query: 241 GANPMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQ 300
           G+NPMPPEFW+L   LP  P  ++CY RL  +PMSYL+GKRFVGP+TPLILQLR+E++T+
Sbjct: 241 GSNPMPPEFWILPSFLPMHPAKMWCYCRLVYMPMSYLYGKRFVGPITPLILQLREELHTE 300

Query: 301 PYNHIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSWAF-KTIRNSALQVAK 360
           PY  I W+ TRH CAKED  +   L Q L WD++  F EP+L  W F K +R  AL+V  
Sbjct: 301 PYEKINWTKTRHLCAKEDIYYPHPLIQDLIWDSLYIFTEPLLTRWPFNKLVRKRALEVTM 360

Query: 361 CHIDYEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHVARIKDYLWIAEDGMKMQ 415
            HI YED NS Y+TIGCVEK L  LACWV+DPNG+A+KKH+AR+ DYLWI+EDGM MQ
Sbjct: 361 KHIHYEDENSRYLTIGCVEKVLCMLACWVEDPNGDAFKKHIARVPDYLWISEDGMTMQ 411

BLAST of Csor.00g208370 vs. ExPASy Swiss-Prot
Match: O82146 (Beta-amyrin synthase 2 OS=Panax ginseng OX=4054 GN=OSCPNY2 PE=2 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 2.0e-159
Identity = 253/417 (60.67%), Postives = 306/417 (73.38%), Query Frame = 0

Query: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKERAEVEEACQNFYQNRFKVQCSS 60
           MWRL   +G NDPYL+S+NNF+GRQTW+F+ D GTP ERAEVEEA  +F+ NR++V+ SS
Sbjct: 1   MWRLMTAKGGNDPYLYSTNNFIGRQTWEFDPDYGTPAERAEVEEARLHFWNNRYQVKPSS 60

Query: 61  NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALINKETASIALRRATKFFAALQSHHG 120
           +  W+ Q L+E+NFKQ IP VKVE   DG+E    I  E A+  LRRA  +F+ALQ+  G
Sbjct: 61  DVLWRMQFLKEKNFKQIIPQVKVE---DGEE----ITYEAATTTLRRAVHYFSALQADDG 120

Query: 121 HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRREILRYAYCHQNEDGGWGLHIEGQS 180
           HWPAEN+GP+F+ PPLV  LYITGHL  +F  EHR EILRY YCHQN+DGGWGLHIEG S
Sbjct: 121 HWPAENAGPLFFLPPLVMCLYITGHLNTVFPAEHRIEILRYIYCHQNDDGGWGLHIEGHS 180

Query: 181 CMLCTVFNYIQLRLLGEEPD---KNECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWE 240
            M CT  +YI +R+LGE  D    N C RARKWILDHG    IPSWGK WLSILG+++W 
Sbjct: 181 TMFCTALSYICMRILGEGRDGGENNACARARKWILDHGSVTAIPSWGKTWLSILGLFDWS 240

Query: 241 GANPMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQ 300
           G+NPMPPEFW+L   LP  P  ++CY R+  +PMSYL+GKRFVGP+TPLILQLR+E+Y Q
Sbjct: 241 GSNPMPPEFWILPPFLPMHPAKMWCYCRMVYMPMSYLYGKRFVGPITPLILQLREELYAQ 300

Query: 301 PYNHIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSWAFKTIRNSALQVAKC 360
            Y+ I W   RH CAKED  +   L Q L WD++  F EP L  W F  +R  ALQ    
Sbjct: 301 AYDEINWRKVRHNCAKEDLYYPHPLIQDLMWDSLYIFTEPFLTRWPFNKLREKALQTTMK 360

Query: 361 HIDYEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHVARIKDYLWIAEDGMKMQ 415
           HI YED NS YITIGCVEK L  LACWV+DPNG+ +K+H+ARI DY+W+AEDGMKMQ
Sbjct: 361 HIHYEDENSRYITIGCVEKVLCMLACWVEDPNGDYFKQHLARIPDYIWVAEDGMKMQ 410

BLAST of Csor.00g208370 vs. NCBI nr
Match: KAG6599055.1 (hypothetical protein SDJN03_08833, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 907 bits (2343), Expect = 0.0
Identity = 420/420 (100.00%), Postives = 420/420 (100.00%), Query Frame = 0

Query: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKERAEVEEACQNFYQNRFKVQCSS 60
           MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKERAEVEEACQNFYQNRFKVQCSS
Sbjct: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKERAEVEEACQNFYQNRFKVQCSS 60

Query: 61  NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALINKETASIALRRATKFFAALQSHHG 120
           NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALINKETASIALRRATKFFAALQSHHG
Sbjct: 61  NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALINKETASIALRRATKFFAALQSHHG 120

Query: 121 HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRREILRYAYCHQNEDGGWGLHIEGQS 180
           HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRREILRYAYCHQNEDGGWGLHIEGQS
Sbjct: 121 HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRREILRYAYCHQNEDGGWGLHIEGQS 180

Query: 181 CMLCTVFNYIQLRLLGEEPDKNECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGAN 240
           CMLCTVFNYIQLRLLGEEPDKNECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGAN
Sbjct: 181 CMLCTVFNYIQLRLLGEEPDKNECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGAN 240

Query: 241 PMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQPYN 300
           PMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQPYN
Sbjct: 241 PMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQPYN 300

Query: 301 HIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSWAFKTIRNSALQVAKCHID 360
           HIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSWAFKTIRNSALQVAKCHID
Sbjct: 301 HIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSWAFKTIRNSALQVAKCHID 360

Query: 361 YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHVARIKDYLWIAEDGMKMQVSITHY 420
           YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHVARIKDYLWIAEDGMKMQVSITHY
Sbjct: 361 YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHVARIKDYLWIAEDGMKMQVSITHY 420

BLAST of Csor.00g208370 vs. NCBI nr
Match: XP_022946464.1 (beta-amyrin synthase-like isoform X4 [Cucurbita moschata])

HSP 1 Score: 865 bits (2235), Expect = 1.10e-312
Identity = 401/414 (96.86%), Postives = 408/414 (98.55%), Query Frame = 0

Query: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKERAEVEEACQNFYQNRFKVQCSS 60
           MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPK+RAEVE+A QNFY+NRFKVQCSS
Sbjct: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKKRAEVEQARQNFYKNRFKVQCSS 60

Query: 61  NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALINKETASIALRRATKFFAALQSHHG 120
           NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALI KET +IALRRATKFFAALQSHHG
Sbjct: 61  NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALIKKETVTIALRRATKFFAALQSHHG 120

Query: 121 HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRREILRYAYCHQNEDGGWGLHIEGQS 180
           HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHR+EILRYAYCHQNEDGGWGLHIEGQS
Sbjct: 121 HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQNEDGGWGLHIEGQS 180

Query: 181 CMLCTVFNYIQLRLLGEEPDKNECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGAN 240
           CMLCTVFNYIQLRLLGEEPDK+ECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGAN
Sbjct: 181 CMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGAN 240

Query: 241 PMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQPYN 300
           PMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVG LTPLILQLRQEIYTQPYN
Sbjct: 241 PMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGTLTPLILQLRQEIYTQPYN 300

Query: 301 HIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSWAFKTIRNSALQVAKCHID 360
           HIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNS AFKTIRN ALQVAKCHID
Sbjct: 301 HIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSSAFKTIRNRALQVAKCHID 360

Query: 361 YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHVARIKDYLWIAEDGMKMQ 414
           YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKH+ARIKDYLWIAEDGMKMQ
Sbjct: 361 YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHLARIKDYLWIAEDGMKMQ 414

BLAST of Csor.00g208370 vs. NCBI nr
Match: XP_022946462.1 (beta-amyrin synthase-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 865 bits (2235), Expect = 1.06e-311
Identity = 401/414 (96.86%), Postives = 408/414 (98.55%), Query Frame = 0

Query: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKERAEVEEACQNFYQNRFKVQCSS 60
           MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPK+RAEVE+A QNFY+NRFKVQCSS
Sbjct: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKKRAEVEQARQNFYKNRFKVQCSS 60

Query: 61  NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALINKETASIALRRATKFFAALQSHHG 120
           NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALI KET +IALRRATKFFAALQSHHG
Sbjct: 61  NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALIKKETVTIALRRATKFFAALQSHHG 120

Query: 121 HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRREILRYAYCHQNEDGGWGLHIEGQS 180
           HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHR+EILRYAYCHQNEDGGWGLHIEGQS
Sbjct: 121 HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQNEDGGWGLHIEGQS 180

Query: 181 CMLCTVFNYIQLRLLGEEPDKNECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGAN 240
           CMLCTVFNYIQLRLLGEEPDK+ECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGAN
Sbjct: 181 CMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGAN 240

Query: 241 PMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQPYN 300
           PMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVG LTPLILQLRQEIYTQPYN
Sbjct: 241 PMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGTLTPLILQLRQEIYTQPYN 300

Query: 301 HIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSWAFKTIRNSALQVAKCHID 360
           HIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNS AFKTIRN ALQVAKCHID
Sbjct: 301 HIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSSAFKTIRNRALQVAKCHID 360

Query: 361 YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHVARIKDYLWIAEDGMKMQ 414
           YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKH+ARIKDYLWIAEDGMKMQ
Sbjct: 361 YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHLARIKDYLWIAEDGMKMQ 414

BLAST of Csor.00g208370 vs. NCBI nr
Match: XP_022946460.1 (beta-amyrin synthase-like isoform X1 [Cucurbita moschata] >XP_022946461.1 beta-amyrin synthase-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 865 bits (2235), Expect = 1.06e-311
Identity = 401/414 (96.86%), Postives = 408/414 (98.55%), Query Frame = 0

Query: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKERAEVEEACQNFYQNRFKVQCSS 60
           MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPK+RAEVE+A QNFY+NRFKVQCSS
Sbjct: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKKRAEVEQARQNFYKNRFKVQCSS 60

Query: 61  NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALINKETASIALRRATKFFAALQSHHG 120
           NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALI KET +IALRRATKFFAALQSHHG
Sbjct: 61  NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALIKKETVTIALRRATKFFAALQSHHG 120

Query: 121 HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRREILRYAYCHQNEDGGWGLHIEGQS 180
           HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHR+EILRYAYCHQNEDGGWGLHIEGQS
Sbjct: 121 HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQNEDGGWGLHIEGQS 180

Query: 181 CMLCTVFNYIQLRLLGEEPDKNECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGAN 240
           CMLCTVFNYIQLRLLGEEPDK+ECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGAN
Sbjct: 181 CMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGAN 240

Query: 241 PMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQPYN 300
           PMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVG LTPLILQLRQEIYTQPYN
Sbjct: 241 PMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGTLTPLILQLRQEIYTQPYN 300

Query: 301 HIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSWAFKTIRNSALQVAKCHID 360
           HIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNS AFKTIRN ALQVAKCHID
Sbjct: 301 HIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSSAFKTIRNRALQVAKCHID 360

Query: 361 YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHVARIKDYLWIAEDGMKMQ 414
           YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKH+ARIKDYLWIAEDGMKMQ
Sbjct: 361 YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHLARIKDYLWIAEDGMKMQ 414

BLAST of Csor.00g208370 vs. NCBI nr
Match: KAG6599054.1 (hypothetical protein SDJN03_08832, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 838 bits (2166), Expect = 8.20e-307
Identity = 387/420 (92.14%), Postives = 401/420 (95.48%), Query Frame = 0

Query: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKERAEVEEACQNFYQNRFKVQCSS 60
           MWRLKLGEGANDPYLFSSNNF+GRQTWDFE DEGTP+ERAEVEEA QN+Y NRFKV CSS
Sbjct: 1   MWRLKLGEGANDPYLFSSNNFMGRQTWDFEPDEGTPEERAEVEEARQNYYHNRFKVPCSS 60

Query: 61  NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALINKETASIALRRATKFFAALQSHHG 120
           + FWKFQ LRERNFKQTIP+VKVEE        A+INKETASIALRRATKFFAALQSHHG
Sbjct: 61  DLFWKFQFLRERNFKQTIPIVKVEE--------AIINKETASIALRRATKFFAALQSHHG 120

Query: 121 HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRREILRYAYCHQNEDGGWGLHIEGQS 180
           HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHR+EILRYAYCHQNEDGGWGLHIEGQS
Sbjct: 121 HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQNEDGGWGLHIEGQS 180

Query: 181 CMLCTVFNYIQLRLLGEEPDKNECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGAN 240
           CMLCTVFNYIQLRLLGEEPDK+ECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGAN
Sbjct: 181 CMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGAN 240

Query: 241 PMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQPYN 300
           PMPPEFWLLGKLLPFIP++LFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQPYN
Sbjct: 241 PMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQPYN 300

Query: 301 HIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSWAFKTIRNSALQVAKCHID 360
           HIKW+PTRHYCAKEDKCFERSLFQKL WD++QY GEPILNS AFK IRN ALQ+AKCHID
Sbjct: 301 HIKWNPTRHYCAKEDKCFERSLFQKLAWDSLQYLGEPILNSMAFKKIRNKALQIAKCHID 360

Query: 361 YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHVARIKDYLWIAEDGMKMQVSITHY 420
           YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHVARIKDYLWIAEDGMKMQVSITHY
Sbjct: 361 YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHVARIKDYLWIAEDGMKMQVSITHY 412

BLAST of Csor.00g208370 vs. ExPASy TrEMBL
Match: A0A6J1G3U8 (Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC111450512 PE=3 SV=1)

HSP 1 Score: 865 bits (2235), Expect = 5.33e-313
Identity = 401/414 (96.86%), Postives = 408/414 (98.55%), Query Frame = 0

Query: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKERAEVEEACQNFYQNRFKVQCSS 60
           MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPK+RAEVE+A QNFY+NRFKVQCSS
Sbjct: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKKRAEVEQARQNFYKNRFKVQCSS 60

Query: 61  NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALINKETASIALRRATKFFAALQSHHG 120
           NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALI KET +IALRRATKFFAALQSHHG
Sbjct: 61  NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALIKKETVTIALRRATKFFAALQSHHG 120

Query: 121 HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRREILRYAYCHQNEDGGWGLHIEGQS 180
           HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHR+EILRYAYCHQNEDGGWGLHIEGQS
Sbjct: 121 HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQNEDGGWGLHIEGQS 180

Query: 181 CMLCTVFNYIQLRLLGEEPDKNECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGAN 240
           CMLCTVFNYIQLRLLGEEPDK+ECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGAN
Sbjct: 181 CMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGAN 240

Query: 241 PMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQPYN 300
           PMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVG LTPLILQLRQEIYTQPYN
Sbjct: 241 PMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGTLTPLILQLRQEIYTQPYN 300

Query: 301 HIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSWAFKTIRNSALQVAKCHID 360
           HIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNS AFKTIRN ALQVAKCHID
Sbjct: 301 HIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSSAFKTIRNRALQVAKCHID 360

Query: 361 YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHVARIKDYLWIAEDGMKMQ 414
           YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKH+ARIKDYLWIAEDGMKMQ
Sbjct: 361 YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHLARIKDYLWIAEDGMKMQ 414

BLAST of Csor.00g208370 vs. ExPASy TrEMBL
Match: A0A6J1G3X4 (Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC111450512 PE=3 SV=1)

HSP 1 Score: 865 bits (2235), Expect = 5.12e-312
Identity = 401/414 (96.86%), Postives = 408/414 (98.55%), Query Frame = 0

Query: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKERAEVEEACQNFYQNRFKVQCSS 60
           MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPK+RAEVE+A QNFY+NRFKVQCSS
Sbjct: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKKRAEVEQARQNFYKNRFKVQCSS 60

Query: 61  NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALINKETASIALRRATKFFAALQSHHG 120
           NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALI KET +IALRRATKFFAALQSHHG
Sbjct: 61  NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALIKKETVTIALRRATKFFAALQSHHG 120

Query: 121 HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRREILRYAYCHQNEDGGWGLHIEGQS 180
           HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHR+EILRYAYCHQNEDGGWGLHIEGQS
Sbjct: 121 HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQNEDGGWGLHIEGQS 180

Query: 181 CMLCTVFNYIQLRLLGEEPDKNECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGAN 240
           CMLCTVFNYIQLRLLGEEPDK+ECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGAN
Sbjct: 181 CMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGAN 240

Query: 241 PMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQPYN 300
           PMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVG LTPLILQLRQEIYTQPYN
Sbjct: 241 PMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGTLTPLILQLRQEIYTQPYN 300

Query: 301 HIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSWAFKTIRNSALQVAKCHID 360
           HIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNS AFKTIRN ALQVAKCHID
Sbjct: 301 HIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSSAFKTIRNRALQVAKCHID 360

Query: 361 YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHVARIKDYLWIAEDGMKMQ 414
           YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKH+ARIKDYLWIAEDGMKMQ
Sbjct: 361 YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHLARIKDYLWIAEDGMKMQ 414

BLAST of Csor.00g208370 vs. ExPASy TrEMBL
Match: A0A6J1G3T6 (Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC111450512 PE=3 SV=1)

HSP 1 Score: 865 bits (2235), Expect = 5.12e-312
Identity = 401/414 (96.86%), Postives = 408/414 (98.55%), Query Frame = 0

Query: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKERAEVEEACQNFYQNRFKVQCSS 60
           MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPK+RAEVE+A QNFY+NRFKVQCSS
Sbjct: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKKRAEVEQARQNFYKNRFKVQCSS 60

Query: 61  NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALINKETASIALRRATKFFAALQSHHG 120
           NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALI KET +IALRRATKFFAALQSHHG
Sbjct: 61  NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALIKKETVTIALRRATKFFAALQSHHG 120

Query: 121 HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRREILRYAYCHQNEDGGWGLHIEGQS 180
           HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHR+EILRYAYCHQNEDGGWGLHIEGQS
Sbjct: 121 HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQNEDGGWGLHIEGQS 180

Query: 181 CMLCTVFNYIQLRLLGEEPDKNECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGAN 240
           CMLCTVFNYIQLRLLGEEPDK+ECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGAN
Sbjct: 181 CMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGAN 240

Query: 241 PMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQPYN 300
           PMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVG LTPLILQLRQEIYTQPYN
Sbjct: 241 PMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGTLTPLILQLRQEIYTQPYN 300

Query: 301 HIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSWAFKTIRNSALQVAKCHID 360
           HIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNS AFKTIRN ALQVAKCHID
Sbjct: 301 HIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSSAFKTIRNRALQVAKCHID 360

Query: 361 YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHVARIKDYLWIAEDGMKMQ 414
           YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKH+ARIKDYLWIAEDGMKMQ
Sbjct: 361 YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHLARIKDYLWIAEDGMKMQ 414

BLAST of Csor.00g208370 vs. ExPASy TrEMBL
Match: A0A6J1KGL7 (Terpene cyclase/mutase family member OS=Cucurbita maxima OX=3661 GN=LOC111493705 PE=3 SV=1)

HSP 1 Score: 829 bits (2141), Expect = 1.06e-298
Identity = 376/414 (90.82%), Postives = 398/414 (96.14%), Query Frame = 0

Query: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKERAEVEEACQNFYQNRFKVQCSS 60
           MWRLKLGEGANDPYLFSSNNFVGRQTWDFE D+GTP+ERAEVEEA QN+YQNRFKVQCSS
Sbjct: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYQNRFKVQCSS 60

Query: 61  NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALINKETASIALRRATKFFAALQSHHG 120
           + FWKFQ LR+RNFKQTIP+VKVEEG DGDEKVA+INKETA+ ALRRAT FFAALQSHHG
Sbjct: 61  DLFWKFQFLRDRNFKQTIPIVKVEEGRDGDEKVAIINKETANNALRRATLFFAALQSHHG 120

Query: 121 HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRREILRYAYCHQNEDGGWGLHIEGQS 180
           HWPAENSGPMFYFPPLVFSLYITGHLG+IFS+ HR EILRY+YCHQN DGGWGLHI+GQS
Sbjct: 121 HWPAENSGPMFYFPPLVFSLYITGHLGVIFSQHHRNEILRYSYCHQNGDGGWGLHIQGQS 180

Query: 181 CMLCTVFNYIQLRLLGEEPDKNECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGAN 240
           CMLCTVFNY+QLRLLGE+PDK+ECFRARKWILDHGGAIYIPSWGKIWLSILGVY+WEGAN
Sbjct: 181 CMLCTVFNYLQLRLLGEQPDKDECFRARKWILDHGGAIYIPSWGKIWLSILGVYQWEGAN 240

Query: 241 PMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQPYN 300
           PMPPEFWLLGKLLPFIP++LFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQ YN
Sbjct: 241 PMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQSYN 300

Query: 301 HIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSWAFKTIRNSALQVAKCHID 360
           +IKWSPTRHYCAK DKCFERSLFQKL WDA+QYFGEPILNSWAFKTIRN ALQ AKCHID
Sbjct: 301 NIKWSPTRHYCAKADKCFERSLFQKLAWDALQYFGEPILNSWAFKTIRNRALQEAKCHID 360

Query: 361 YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHVARIKDYLWIAEDGMKMQ 414
           YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKH+ARIKDYLW+ EDGMKMQ
Sbjct: 361 YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHLARIKDYLWVGEDGMKMQ 414

BLAST of Csor.00g208370 vs. ExPASy TrEMBL
Match: A0A6J1KEZ8 (Terpene cyclase/mutase family member OS=Cucurbita maxima OX=3661 GN=LOC111493705 PE=3 SV=1)

HSP 1 Score: 829 bits (2141), Expect = 1.00e-297
Identity = 376/414 (90.82%), Postives = 398/414 (96.14%), Query Frame = 0

Query: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKERAEVEEACQNFYQNRFKVQCSS 60
           MWRLKLGEGANDPYLFSSNNFVGRQTWDFE D+GTP+ERAEVEEA QN+YQNRFKVQCSS
Sbjct: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYQNRFKVQCSS 60

Query: 61  NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALINKETASIALRRATKFFAALQSHHG 120
           + FWKFQ LR+RNFKQTIP+VKVEEG DGDEKVA+INKETA+ ALRRAT FFAALQSHHG
Sbjct: 61  DLFWKFQFLRDRNFKQTIPIVKVEEGRDGDEKVAIINKETANNALRRATLFFAALQSHHG 120

Query: 121 HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRREILRYAYCHQNEDGGWGLHIEGQS 180
           HWPAENSGPMFYFPPLVFSLYITGHLG+IFS+ HR EILRY+YCHQN DGGWGLHI+GQS
Sbjct: 121 HWPAENSGPMFYFPPLVFSLYITGHLGVIFSQHHRNEILRYSYCHQNGDGGWGLHIQGQS 180

Query: 181 CMLCTVFNYIQLRLLGEEPDKNECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGAN 240
           CMLCTVFNY+QLRLLGE+PDK+ECFRARKWILDHGGAIYIPSWGKIWLSILGVY+WEGAN
Sbjct: 181 CMLCTVFNYLQLRLLGEQPDKDECFRARKWILDHGGAIYIPSWGKIWLSILGVYQWEGAN 240

Query: 241 PMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQPYN 300
           PMPPEFWLLGKLLPFIP++LFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQ YN
Sbjct: 241 PMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQSYN 300

Query: 301 HIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSWAFKTIRNSALQVAKCHID 360
           +IKWSPTRHYCAK DKCFERSLFQKL WDA+QYFGEPILNSWAFKTIRN ALQ AKCHID
Sbjct: 301 NIKWSPTRHYCAKADKCFERSLFQKLAWDALQYFGEPILNSWAFKTIRNRALQEAKCHID 360

Query: 361 YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHVARIKDYLWIAEDGMKMQ 414
           YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKH+ARIKDYLW+ EDGMKMQ
Sbjct: 361 YEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHLARIKDYLWVGEDGMKMQ 414

BLAST of Csor.00g208370 vs. TAIR 10
Match: AT1G78950.1 (Terpenoid cyclases family protein )

HSP 1 Score: 562.4 bits (1448), Expect = 3.1e-160
Identity = 259/419 (61.81%), Postives = 309/419 (73.75%), Query Frame = 0

Query: 1   MWRLKLGEG-ANDPYLFSSNNFVGRQTWDFEVDEGTPKERAEVEEACQNFYQNRFKVQCS 60
           MWRLK+GEG  +DPYLF++NNF GRQTW+F+ D G+P+ER  V EA + FY NRF V+ S
Sbjct: 1   MWRLKIGEGNGDDPYLFTTNNFAGRQTWEFDPDGGSPEERHSVVEARRIFYDNRFHVKAS 60

Query: 61  SNFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALINKETASIALRRATKFFAALQSHH 120
           S+  W+ Q LRE+ F+Q I  VKVE+     EKV     ETA+ ALRR   FF+ALQ+  
Sbjct: 61  SDLLWRMQFLREKKFEQRIAPVKVED----SEKVTF---ETATSALRRGIHFFSALQASD 120

Query: 121 GHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRREILRYAYCHQNEDGGWGLHIEGQ 180
           GHWPAEN+GP+F+ PPLVF LYITGHL  +F+ EHR+EILRY YCHQ EDGGWGLHIEG 
Sbjct: 121 GHWPAENAGPLFFLPPLVFCLYITGHLDEVFTSEHRKEILRYIYCHQKEDGGWGLHIEGH 180

Query: 181 SCMLCTVFNYIQLRLLGEEPD---KNECFRARKWILDHGGAIYIPSWGKIWLSILGVYEW 240
           S M CT  NYI +R+LGE PD    N C RAR+WIL HGG  YIPSWGK WLSILGV++W
Sbjct: 181 STMFCTTLNYICMRILGESPDGGHDNACGRAREWILSHGGVTYIPSWGKTWLSILGVFDW 240

Query: 241 EGANPMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYT 300
            G+NPMPPEFW+L    P  P  ++ Y R+  LPMSYL+GKRFVGP+T LILQLR+E+Y 
Sbjct: 241 SGSNPMPPEFWILPSFFPVHPAKMWSYCRMVYLPMSYLYGKRFVGPITSLILQLRKELYL 300

Query: 301 QPYNHIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSWAF-KTIRNSALQVA 360
           QPY  I W   RH CAKED  + R L Q+L WD++  F EP L  W F K +R  ALQ+A
Sbjct: 301 QPYEEINWMKVRHLCAKEDTYYPRPLVQELVWDSLYIFAEPFLARWPFNKLLREKALQLA 360

Query: 361 KCHIDYEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHVARIKDYLWIAEDGMKMQ 415
             HI YED NS YITIGCVEK L  LACWV+DPNG+ +KKH++RI DYLW+AEDGMKMQ
Sbjct: 361 MKHIHYEDENSRYITIGCVEKVLCMLACWVEDPNGDYFKKHLSRISDYLWMAEDGMKMQ 412

BLAST of Csor.00g208370 vs. TAIR 10
Match: AT1G78955.1 (camelliol C synthase 1 )

HSP 1 Score: 548.5 bits (1412), Expect = 4.6e-156
Identity = 251/419 (59.90%), Postives = 304/419 (72.55%), Query Frame = 0

Query: 1   MWRLKLGEG-ANDPYLFSSNNFVGRQTWDFEVDEGTPKERAEVEEACQNFYQNRFKVQCS 60
           MW+LK+  G   +PYLFS+NNF+GRQTW+F+ D GT +E A VEEA + FY +RF+V+ S
Sbjct: 1   MWKLKIANGNKEEPYLFSTNNFLGRQTWEFDPDAGTVEELAAVEEARRKFYDDRFRVKAS 60

Query: 61  SNFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALINKETASIALRRATKFFAALQSHH 120
           S+  W+ Q L+E+ F+Q IP  KVE+  +       I  E A+ ALR+   F +ALQ+  
Sbjct: 61  SDLIWRMQFLKEKKFEQVIPPAKVEDANN-------ITSEIATNALRKGVNFLSALQASD 120

Query: 121 GHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRREILRYAYCHQNEDGGWGLHIEGQ 180
           GHWPAEN+GP+F+ PPLVF LY+TGHL  IF+++HRRE+LRY YCHQNEDGGWGLHIEG 
Sbjct: 121 GHWPAENAGPLFFLPPLVFCLYVTGHLHEIFTQDHRREVLRYIYCHQNEDGGWGLHIEGN 180

Query: 181 SCMLCTVFNYIQLRLLGEEPD---KNECFRARKWILDHGGAIYIPSWGKIWLSILGVYEW 240
           S M CT  NYI +R+LGE P+    N C RAR WILDHGGA YIPSWGK WLSILGV++W
Sbjct: 181 STMFCTTLNYICMRILGEGPNGGPGNACKRARDWILDHGGATYIPSWGKTWLSILGVFDW 240

Query: 241 EGANPMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYT 300
            G+NPMPPEFW+L   LP  P  ++CY RL  +PMSYL+GKRFVGP++PLILQLR+EIY 
Sbjct: 241 SGSNPMPPEFWILPSFLPIHPAKMWCYCRLVYMPMSYLYGKRFVGPISPLILQLREEIYL 300

Query: 301 QPYNHIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSWAF-KTIRNSALQVA 360
           QPY  I W+  RH CAKED        Q + W+ +  F EP L  W F K +R  AL VA
Sbjct: 301 QPYAKINWNRARHLCAKEDAYCPHPQIQDVIWNCLYIFTEPFLACWPFNKLLREKALGVA 360

Query: 361 KCHIDYEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHVARIKDYLWIAEDGMKMQ 415
             HI YED NS YITIGCVEK L  LACWV+DPNG  +KKH+ RI DYLWIAEDGMKMQ
Sbjct: 361 MKHIHYEDENSRYITIGCVEKALCMLACWVEDPNGIHFKKHLLRISDYLWIAEDGMKMQ 412

BLAST of Csor.00g208370 vs. TAIR 10
Match: AT1G78960.1 (lupeol synthase 2 )

HSP 1 Score: 544.3 bits (1401), Expect = 8.7e-155
Identity = 249/419 (59.43%), Postives = 307/419 (73.27%), Query Frame = 0

Query: 1   MWRLKLGEG-ANDPYLFSSNNFVGRQTWDFEVDEGTPKERAEVEEACQNFYQNRFKVQCS 60
           MW+LK+GEG   DPYLFSSNNFVGRQTW+F+   GTP+ERA VE+A +N+  NR +V+  
Sbjct: 1   MWKLKIGEGNGEDPYLFSSNNFVGRQTWEFDPKAGTPEERAAVEDARRNYLDNRPRVKGC 60

Query: 61  SNFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALINKETASIALRRATKFFAALQSHH 120
           S+  W+ Q L+E  F+Q IP VK+++G +G      I  + A+ ALRRA  F++ALQS  
Sbjct: 61  SDLLWRMQFLKEAKFEQVIPPVKIDDG-EG------ITYKNATDALRRAVSFYSALQSSD 120

Query: 121 GHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRREILRYAYCHQNEDGGWGLHIEGQ 180
           GHWPAE +G +F+ PPLVF  YITGHL  IF  EHR+E+LR+ YCHQNEDGGWGLHIEG+
Sbjct: 121 GHWPAEITGTLFFLPPLVFCFYITGHLEKIFDAEHRKEMLRHIYCHQNEDGGWGLHIEGK 180

Query: 181 SCMLCTVFNYIQLRLLGEEPD---KNECFRARKWILDHGGAIYIPSWGKIWLSILGVYEW 240
           S M CTV NYI LR+LGE P+    N C RAR+WILDHGG  YIPSWGKIWLSILG+Y+W
Sbjct: 181 SVMFCTVLNYICLRMLGEGPNGGRNNACKRARQWILDHGGVTYIPSWGKIWLSILGIYDW 240

Query: 241 EGANPMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYT 300
            G NPMPPE WLL    P       CY+R+  +PMSYL+GKRFVGPLTPLI+ LR+E++ 
Sbjct: 241 SGTNPMPPEIWLLPSFFPIHLGKTLCYTRMVYMPMSYLYGKRFVGPLTPLIMLLRKELHL 300

Query: 301 QPYNHIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSWAF-KTIRNSALQVA 360
           QPY  I W+  R  CAKED  +   L Q L WD +  F EPIL +W   K +R  AL+VA
Sbjct: 301 QPYEEINWNKARRLCAKEDMIYPHPLVQDLLWDTLHNFVEPILTNWPLKKLVREKALRVA 360

Query: 361 KCHIDYEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHVARIKDYLWIAEDGMKMQ 415
             HI YED NSHYITIGCVEK L  LACW+++PNG+ +KKH+ARI D++W+AEDG+KMQ
Sbjct: 361 MEHIHYEDENSHYITIGCVEKVLCMLACWIENPNGDHFKKHLARIPDFMWVAEDGLKMQ 412

BLAST of Csor.00g208370 vs. TAIR 10
Match: AT1G66960.1 (Terpenoid cyclases family protein )

HSP 1 Score: 531.9 bits (1369), Expect = 4.5e-151
Identity = 247/419 (58.95%), Postives = 300/419 (71.60%), Query Frame = 0

Query: 1   MWRLKLGEG-ANDPYLFSSNNFVGRQTWDFEVDEGTPKERAEVEEACQNFYQNRFKVQCS 60
           MWRLK+GEG   DPYLFSSNNFVGRQTW+F+   GT +ER  VEEA ++F+ NR +V+ S
Sbjct: 1   MWRLKVGEGKGKDPYLFSSNNFVGRQTWEFDPKAGTREERTAVEEARRSFFDNRSRVKPS 60

Query: 61  SNFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALINKETASIALRRATKFFAALQSHH 120
           S+  WK Q L+E  F+Q IP VK    IDG E    I  E A+ ALRR   F +ALQ+  
Sbjct: 61  SDLLWKMQFLKEAKFEQVIPPVK----IDGGE---AITYEKATNALRRGVAFLSALQASD 120

Query: 121 GHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRREILRYAYCHQNEDGGWGLHIEGQ 180
           GHWP E +GP+   PPLVF LYITGHL  +F  EHR+E+LRY YCHQNEDGGWG HIE +
Sbjct: 121 GHWPGEFTGPLCMLPPLVFCLYITGHLEEVFDAEHRKEMLRYIYCHQNEDGGWGFHIESK 180

Query: 181 SCMLCTVFNYIQLRLLGEEPD---KNECFRARKWILDHGGAIYIPSWGKIWLSILGVYEW 240
           S M  T  NYI LR+LG  PD   +N C RAR+WIL HGG IYIP WGK+WLS+LG+Y+W
Sbjct: 181 SIMFTTTLNYICLRILGVGPDGGLENACKRARQWILSHGGVIYIPCWGKVWLSVLGIYDW 240

Query: 241 EGANPMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYT 300
            G NPMPPE WLL   LP      F Y+R+T +P+SYL+GK+FVG +TPLI+QLR+E++ 
Sbjct: 241 SGVNPMPPEIWLLPYFLPIHLGKAFSYTRITYMPISYLYGKKFVGQITPLIMQLREELHL 300

Query: 301 QPYNHIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSWAF-KTIRNSALQVA 360
           QPY  I W+  RH CAKEDK +   L Q L WDA+  F EP+L SW   K +R  ALQVA
Sbjct: 301 QPYEEINWNKARHLCAKEDKYYPHPLVQDLIWDALHTFVEPLLASWPINKLVRKKALQVA 360

Query: 361 KCHIDYEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHVARIKDYLWIAEDGMKMQ 415
             HI YED NSHYITIGC+EK L  LACW+D+P+G  +KKH++RI D +W+AEDGMKMQ
Sbjct: 361 MKHIHYEDENSHYITIGCIEKNLCMLACWIDNPDGNHFKKHLSRIPDMMWVAEDGMKMQ 412

BLAST of Csor.00g208370 vs. TAIR 10
Match: AT1G78970.2 (lupeol synthase 1 )

HSP 1 Score: 530.4 bits (1365), Expect = 1.3e-150
Identity = 241/416 (57.93%), Postives = 299/416 (71.88%), Query Frame = 0

Query: 1   MWRLKLGEG-ANDPYLFSSNNFVGRQTWDFEVDEGTPKERAEVEEACQNFYQNRFKVQCS 60
           MW+LK+G+G   DP+LFSSNNFVGRQTW F+   G+P+ERA VEEA + F  NRF+V+  
Sbjct: 1   MWKLKIGKGNGEDPHLFSSNNFVGRQTWKFDHKAGSPEERAAVEEARRGFLDNRFRVKGC 60

Query: 61  SNFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALINKETASIALRRATKFFAALQSHH 120
           S+  W+ Q LRE+ F+Q IP +K          +  I  ET + ALRR  ++F ALQ+  
Sbjct: 61  SDLLWRMQFLREKKFEQGIPQLKA-------TNIEEITYETTTNALRRGVRYFTALQASD 120

Query: 121 GHWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRREILRYAYCHQNEDGGWGLHIEGQ 180
           GHWP E +GP+F+ PPL+F LYITGHL  +F  EHR+E+LR+ YCHQNEDGGWGLHIE +
Sbjct: 121 GHWPGEITGPLFFLPPLIFCLYITGHLEEVFDAEHRKEMLRHIYCHQNEDGGWGLHIESK 180

Query: 181 SCMLCTVFNYIQLRLLGEEPDKNECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGA 240
           S M CTV NYI LR+LGE P+++ C RAR+WILD GG I+IPSWGK WLSILGVY+W G 
Sbjct: 181 SVMFCTVLNYICLRMLGENPEQDACKRARQWILDRGGVIFIPSWGKFWLSILGVYDWSGT 240

Query: 241 NPMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQPY 300
           NP PPE  +L   LP  P  + CYSR+  +PMSYL+GKRFVGP+TPLIL LR+E+Y +PY
Sbjct: 241 NPTPPELLMLPSFLPIHPGKILCYSRMVSIPMSYLYGKRFVGPITPLILLLREELYLEPY 300

Query: 301 NHIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSWAF-KTIRNSALQVAKCH 360
             I W  +R   AKED  +   L Q L  D +Q F EP+L  W   K +R  ALQ+   H
Sbjct: 301 EEINWKKSRRLYAKEDMYYAHPLVQDLLSDTLQNFVEPLLTRWPLNKLVREKALQLTMKH 360

Query: 361 IDYEDHNSHYITIGCVEKPLFTLACWVDDPNGEAYKKHVARIKDYLWIAEDGMKMQ 415
           I YED NSHYITIGCVEK L  LACWV++PNG+ +KKH+ARI DY+W+AEDGMKMQ
Sbjct: 361 IHYEDENSHYITIGCVEKVLCMLACWVENPNGDYFKKHLARIPDYMWVAEDGMKMQ 409

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q2XPU74.1e-16562.11Lupeol synthase OS=Ricinus communis OX=3988 PE=1 SV=1[more]
Q8W3Z11.0e-16361.24Beta-amyrin synthase OS=Betula platyphylla OX=78630 GN=OSCBPY PE=1 SV=1[more]
E7DN636.5e-16361.63Beta-amyrin synthase OS=Solanum lycopersicum OX=4081 GN=TTS1 PE=1 SV=1[more]
Q9LRH83.9e-16061.00Beta-amyrin synthase OS=Pisum sativum OX=3888 GN=OSCPSY PE=2 SV=1[more]
O821462.0e-15960.67Beta-amyrin synthase 2 OS=Panax ginseng OX=4054 GN=OSCPNY2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAG6599055.10.0100.00hypothetical protein SDJN03_08833, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022946464.11.10e-31296.86beta-amyrin synthase-like isoform X4 [Cucurbita moschata][more]
XP_022946462.11.06e-31196.86beta-amyrin synthase-like isoform X3 [Cucurbita moschata][more]
XP_022946460.11.06e-31196.86beta-amyrin synthase-like isoform X1 [Cucurbita moschata] >XP_022946461.1 beta-a... [more]
KAG6599054.18.20e-30792.14hypothetical protein SDJN03_08832, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
A0A6J1G3U85.33e-31396.86Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC1114505... [more]
A0A6J1G3X45.12e-31296.86Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC1114505... [more]
A0A6J1G3T65.12e-31296.86Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC1114505... [more]
A0A6J1KGL71.06e-29890.82Terpene cyclase/mutase family member OS=Cucurbita maxima OX=3661 GN=LOC111493705... [more]
A0A6J1KEZ81.00e-29790.82Terpene cyclase/mutase family member OS=Cucurbita maxima OX=3661 GN=LOC111493705... [more]
Match NameE-valueIdentityDescription
AT1G78950.13.1e-16061.81Terpenoid cyclases family protein [more]
AT1G78955.14.6e-15659.90camelliol C synthase 1 [more]
AT1G78960.18.7e-15559.43lupeol synthase 2 [more]
AT1G66960.14.5e-15158.95Terpenoid cyclases family protein [more]
AT1G78970.21.3e-15057.93lupeol synthase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032697Squalene cyclase, N-terminalPFAMPF13249SQHop_cyclase_Ncoord: 106..359
e-value: 4.1E-38
score: 131.1
NoneNo IPR availableGENE3D1.50.10.20coord: 126..416
e-value: 1.2E-120
score: 404.0
NoneNo IPR availablePANTHERPTHR11764:SF69TERPENE CYCLASE/MUTASE FAMILY MEMBERcoord: 1..415
IPR018333Squalene cyclasePANTHERPTHR11764TERPENE CYCLASE/MUTASE FAMILY MEMBERcoord: 1..415
IPR008930Terpenoid cyclases/protein prenyltransferase alpha-alpha toroidSUPERFAMILY48239Terpenoid cyclases/Protein prenyltransferasescoord: 128..413

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g208370.m01Csor.00g208370.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016104 triterpenoid biosynthetic process
cellular_component GO:0005811 lipid droplet
molecular_function GO:0042300 beta-amyrin synthase activity
molecular_function GO:0000250 lanosterol synthase activity
molecular_function GO:0016866 intramolecular transferase activity