Csor.00g201750 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g201750
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	aspartic proteinase PCS1-like
Location	Csor_Chr06: 1466039 .. 1468685 (+)
RNA-Seq Expression	Csor.00g201750
Synteny	Csor.00g201750

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTCCTTATGTTCATTTCCACGCATTTCTTCTGCAGATCCCATCAAGCATCTCGCCGCCGCCCCCTTTCCGCCGTCCAATCACCCGATAAGACCATCGGCGTTGTCTCTCCGTCAAAGCAGCCGCAACCAGAAGAGAACTTCTACGATTGTCGCCGCCATCGGAGACGTCTCCGCTGACGGCACCACGTATTTGATCGCCGGCGCCGTCGCTGTGGCTCTCGTTGGTACCGCCTTCCCTATACTCTTCTCTCGCAAAGACCTGTAAGCAAAATCTCAATTTCTCCTATAAACCATTTGTTCATATTGAACTCATTTTAAAAACGCCGTTTAATACCGCTGTTTGAATGGTCTGGTCCTCCGTAGGTGCCCGGTATGCGACGGCGCAGGGTTTGTCCGGAAGTCGGGGGCGGCGCTGAGGGCGAATGCCGCTCGTAAAGACCAAGCTCAGATCGTTTGTTCTCGTTGCAATGGCCTGGGCAAGCTCAATCAAGTGGACAAATAAATTACTTTATTTTATTTTATATTTTCCGCCCTTTCCTAATGTGACTGTAATATAATATTAAAATATATTATAAGTCTATGTACCTTGAGCTTATAAACATTTAGACTTATAATATATTTTAATACCATACATTGTACTAAAATCTCATGATTTTAATTCAGAAACCTTCCAATTATTAATTTAATGATTTTGCTGATCTGGCAGGCAATATCCACGTGTCAACTCAACGTTGACTCTGGTTCGGCGACCCATTTACTGACTGTTTCTTTTCCACGTGTTATTTTCATCAAAATATATATTTATAAAATAAAAAATTATAAATTCAATTAATATTGCCAAAAATAATAGAGTTAAATATGTTGCATTCTTGCACTATAATTATTATTTTATTATTCAATGAATTGAAACACCTAGGTGTCCTTTTCCAATAATTTCCCCAAATAGGGTAGCCAAATTTTACATTTAATTTTAATTTAAAAACAATTTATAGCCTTCTTAATTACAAATTTATTATTTTTAAATTTATTTAACTTTTGATTTTGAATTTAATAGATCTATTTAATTATTATTTTTTGGAATTTATTGATTTTCTTTTAATAATTGGTCTTTTTTATTGTCGGATCTTCGCAGGGAAGAAGTTTCTCAAAGGATTTCAAAGATTTTATGAACAATCTGTTCTAATAAACCAAAACAGAGCGCACGAATTTCCTCAGATTCTTTTCACTCTGCTTCTTCCGACAGCCCCAAAAAACTTGGAATTCCAGTTTTCTAGGAAGCAAAGAAGAACAAAATGAGGGATTATTGTATAAGTTTAAATCCCTTGAACAACCCATTTCTCAAATATTGTTTCTCTTTCTTCTTAATTTCTCTGGTTTTTGTCTTTCAAAATCCATTACTCTGTTTCTCATTGAACCCAGCCCTTATTTTGCCCCTCAAAACCCAGGTGATTCCGCCTGAATCCGTCCCGCGATCTCCCGACAAGCTCCCTTTCCGGCATAACGTCAGCCTTACTGTCTCTCTGACTGTCGGAACGCCGCCGCAGAATGTCACGATGGTCATCGACACTGGCAGCGAACTCTCATGGCTCCACTGTAACAGATCACAAAACTCCTCTTCTTCATCTTCAACATTCAACCCAGTCCAGTCCTCTTCGTACACTCCTATCCCTTGTTCCTCCTCCACGTGTACCGACCAAACAAGGGATTTTCCGATTCCGGCATCGTGTGATTCAAATCAGCTCTGTCACGCTACTCTGTCTTACGCCGACGCCTCTTCTTCAGAAGGAAATCTCGCCGCCGATATGTTTTACATCGGCAATTCCGGTATTCCGAATGTCGTTTTCGGCTGTATGGATTCAATTTTCAGTTCCAACAATGAAGAAGACTCCAAAAACACTGGATTAATGGGTATGAATCGTGGATCTTTGTCTTTTGTTTCTCAAATGGGTTTCCCCAAATTTTCCTATTGCATATCGGAATATGATTTTCCCGGTTTGTTATTACTCGGAGATGCCAATTTTTCATGGTTGGCTCCGTTGAATTACACTCCAATGATCCAAATATCCACGCCATTGCCGTATTTCGATCGAGTCGCTTACACGGTTCAATTGCAAGGAATCAAAGTTTCCAACAAGTTACTTCCAATACCGGAATCGGTCTTCGAACCGGACCACACCGGCGCCGGTCAGACCATGGTCGATTCAGGCACGCAGTTCACTTTCCTTCTCGGGCAAGCTTACACCGTTCTCCGAGACGAGTTCCTGAACCAAACCGCCGGTTCGCTTCGTGTTTTCGAGGATCCGAATTTCGTTTTCCAAGGAGCCATGGATCTTTGCTACCGAGTTCCAACGAATCATACCCGTCTCCCGCCGCTGCCGGCGGTGACGCTGGTGTTTCGCGGCGCCGAGATGACAGTTACCGGCGACCGTATTCTGTACAGAGTGGCCGGAGAAATAAGAGGGAACGATACGATTTATTGCTTTACATTCGGGAATTCAGATCTTCTGGGCGTTGAAGCGTACGTGATTGGGCATCTCCATCAACAAAACGTGTGGATGGAATTCGATCTGAAAAAATCGCGGATCGGGTTGGCGGAGATCCGGTGCGATTTAGCGGGTCAGAAATTGGGCATGGGCCTGTAA

mRNA sequence

ATGGCGTCCTTATGTTCATTTCCACGCATTTCTTCTGCAGATCCCATCAAGCATCTCGCCGCCGCCCCCTTTCCGCCGTCCAATCACCCGATAAGACCATCGGCGTTGTCTCTCCGTCAAAGCAGCCGCAACCAGAAGAGAACTTCTACGATTGTCGCCGCCATCGGAGACGTCTCCGCTGACGGCACCACGTATTTGATCGCCGGCGCCGTCGCTGTGGCTCTCGTTGGTACCGCCTTCCCTATACTCTTCTCTCGCAAAGACCTGTGCCCGGTATGCGACGGCGCAGGGTTTGTCCGGAAGTCGGGGGCGGCGCTGAGGGCGAATGCCGCTCGTAAAGACCAAGCTCAGATCGTTTGTTCTCGTTGCAATGGCCTGGGCAAGCTCAATCAAGTGATTCCGCCTGAATCCGTCCCGCGATCTCCCGACAAGCTCCCTTTCCGGCATAACGTCAGCCTTACTGTCTCTCTGACTGTCGGAACGCCGCCGCAGAATGTCACGATGGTCATCGACACTGGCAGCGAACTCTCATGGCTCCACTGTAACAGATCACAAAACTCCTCTTCTTCATCTTCAACATTCAACCCAGTCCAGTCCTCTTCGTACACTCCTATCCCTTGTTCCTCCTCCACGTGTACCGACCAAACAAGGGATTTTCCGATTCCGGCATCGTGTGATTCAAATCAGCTCTGTCACGCTACTCTGTCTTACGCCGACGCCTCTTCTTCAGAAGGAAATCTCGCCGCCGATATGTTTTACATCGGCAATTCCGGTATTCCGAATGTCGTTTTCGGCTGTATGGATTCAATTTTCAGTTCCAACAATGAAGAAGACTCCAAAAACACTGGATTAATGGGTATGAATCGTGGATCTTTGTCTTTTGTTTCTCAAATGGGTTTCCCCAAATTTTCCTATTGCATATCGGAATATGATTTTCCCGGTTTGTTATTACTCGGAGATGCCAATTTTTCATGGTTGGCTCCGTTGAATTACACTCCAATGATCCAAATATCCACGCCATTGCCGTATTTCGATCGAGTCGCTTACACGGTTCAATTGCAAGGAATCAAAGTTTCCAACAAGTTACTTCCAATACCGGAATCGGTCTTCGAACCGGACCACACCGGCGCCGGTCAGACCATGGTCGATTCAGGCACGCAGTTCACTTTCCTTCTCGGGCAAGCTTACACCGTTCTCCGAGACGAGTTCCTGAACCAAACCGCCGGTTCGCTTCGTGTTTTCGAGGATCCGAATTTCGTTTTCCAAGGAGCCATGGATCTTTGCTACCGAGTTCCAACGAATCATACCCGTCTCCCGCCGCTGCCGGCGGTGACGCTGGTGTTTCGCGGCGCCGAGATGACAGTTACCGGCGACCGTATTCTGTACAGAGTGGCCGGAGAAATAAGAGGGAACGATACGATTTATTGCTTTACATTCGGGAATTCAGATCTTCTGGGCGTTGAAGCGTACGTGATTGGGCATCTCCATCAACAAAACGTGTGGATGGAATTCGATCTGAAAAAATCGCGGATCGGGTTGGCGGAGATCCGGTGCGATTTAGCGGGTCAGAAATTGGGCATGGGCCTGTAA

Coding sequence (CDS)

ATGGCGTCCTTATGTTCATTTCCACGCATTTCTTCTGCAGATCCCATCAAGCATCTCGCCGCCGCCCCCTTTCCGCCGTCCAATCACCCGATAAGACCATCGGCGTTGTCTCTCCGTCAAAGCAGCCGCAACCAGAAGAGAACTTCTACGATTGTCGCCGCCATCGGAGACGTCTCCGCTGACGGCACCACGTATTTGATCGCCGGCGCCGTCGCTGTGGCTCTCGTTGGTACCGCCTTCCCTATACTCTTCTCTCGCAAAGACCTGTGCCCGGTATGCGACGGCGCAGGGTTTGTCCGGAAGTCGGGGGCGGCGCTGAGGGCGAATGCCGCTCGTAAAGACCAAGCTCAGATCGTTTGTTCTCGTTGCAATGGCCTGGGCAAGCTCAATCAAGTGATTCCGCCTGAATCCGTCCCGCGATCTCCCGACAAGCTCCCTTTCCGGCATAACGTCAGCCTTACTGTCTCTCTGACTGTCGGAACGCCGCCGCAGAATGTCACGATGGTCATCGACACTGGCAGCGAACTCTCATGGCTCCACTGTAACAGATCACAAAACTCCTCTTCTTCATCTTCAACATTCAACCCAGTCCAGTCCTCTTCGTACACTCCTATCCCTTGTTCCTCCTCCACGTGTACCGACCAAACAAGGGATTTTCCGATTCCGGCATCGTGTGATTCAAATCAGCTCTGTCACGCTACTCTGTCTTACGCCGACGCCTCTTCTTCAGAAGGAAATCTCGCCGCCGATATGTTTTACATCGGCAATTCCGGTATTCCGAATGTCGTTTTCGGCTGTATGGATTCAATTTTCAGTTCCAACAATGAAGAAGACTCCAAAAACACTGGATTAATGGGTATGAATCGTGGATCTTTGTCTTTTGTTTCTCAAATGGGTTTCCCCAAATTTTCCTATTGCATATCGGAATATGATTTTCCCGGTTTGTTATTACTCGGAGATGCCAATTTTTCATGGTTGGCTCCGTTGAATTACACTCCAATGATCCAAATATCCACGCCATTGCCGTATTTCGATCGAGTCGCTTACACGGTTCAATTGCAAGGAATCAAAGTTTCCAACAAGTTACTTCCAATACCGGAATCGGTCTTCGAACCGGACCACACCGGCGCCGGTCAGACCATGGTCGATTCAGGCACGCAGTTCACTTTCCTTCTCGGGCAAGCTTACACCGTTCTCCGAGACGAGTTCCTGAACCAAACCGCCGGTTCGCTTCGTGTTTTCGAGGATCCGAATTTCGTTTTCCAAGGAGCCATGGATCTTTGCTACCGAGTTCCAACGAATCATACCCGTCTCCCGCCGCTGCCGGCGGTGACGCTGGTGTTTCGCGGCGCCGAGATGACAGTTACCGGCGACCGTATTCTGTACAGAGTGGCCGGAGAAATAAGAGGGAACGATACGATTTATTGCTTTACATTCGGGAATTCAGATCTTCTGGGCGTTGAAGCGTACGTGATTGGGCATCTCCATCAACAAAACGTGTGGATGGAATTCGATCTGAAAAAATCGCGGATCGGGTTGGCGGAGATCCGGTGCGATTTAGCGGGTCAGAAATTGGGCATGGGCCTGTAA

Protein sequence

MASLCSFPRISSADPIKHLAAAPFPPSNHPIRPSALSLRQSSRNQKRTSTIVAAIGDVSADGTTYLIAGAVAVALVGTAFPILFSRKDLCPVCDGAGFVRKSGAALRANAARKDQAQIVCSRCNGLGKLNQVIPPESVPRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPVQSSSYTPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADMFYIGNSGIPNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFPGLLLLGDANFSWLAPLNYTPMIQISTPLPYFDRVAYTVQLQGIKVSNKLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGQAYTVLRDEFLNQTAGSLRVFEDPNFVFQGAMDLCYRVPTNHTRLPPLPAVTLVFRGAEMTVTGDRILYRVAGEIRGNDTIYCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL

Homology

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BLAST of Csor.00g201750 vs. ExPASy Swiss-Prot
Match: Q9LZL3 (Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 1.4e-159
Identity = 278/394 (70.56%), Postives = 328/394 (83.25%), Query Frame = 0


Query: 140 RSPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPVQS 199
           R  DKL F HNV+LTV+LTVGTPPQN++MVIDTGSELSWL CNRS N +  ++ F+P +S
Sbjct: 60  RPTDKLHFHHNVTLTVTLTVGTPPQNISMVIDTGSELSWLRCNRSSNPNPVNN-FDPTRS 119

Query: 200 SSYTPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADMFYIGNS-G 259
           SSY+PIPCSS TC  +TRDF IPASCDS++LCHATLSYADASSSEGNLAA++F+ GNS  
Sbjct: 120 SSYSPIPCSSPTCRTRTRDFLIPASCDSDKLCHATLSYADASSSEGNLAAEIFHFGNSTN 179

Query: 260 IPNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCIS-EYDFPGLLL 319
             N++FGCM S+  S+ EED+K TGL+GMNRGSLSF+SQMGFPKFSYCIS   DFPG LL
Sbjct: 180 DSNLIFGCMGSVSGSDPEEDTKTTGLLGMNRGSLSFISQMGFPKFSYCISGTDDFPGFLL 239

Query: 320 LGDANFSWLAPLNYTPMIQISTPLPYFDRVAYTVQLQGIKVSNKLLPIPESVFEPDHTGA 379
           LGD+NF+WL PLNYTP+I+ISTPLPYFDRVAYTVQL GIKV+ KLLPIP+SV  PDHTGA
Sbjct: 240 LGDSNFTWLTPLNYTPLIRISTPLPYFDRVAYTVQLTGIKVNGKLLPIPKSVLVPDHTGA 299

Query: 380 GQTMVDSGTQFTFLLGQAYTVLRDEFLNQTAGSLRVFEDPNFVFQGAMDLCYRVPTNHTR 439
           GQTMVDSGTQFTFLLG  YT LR  FLN+T G L V+EDP+FVFQG MDLCYR+     R
Sbjct: 300 GQTMVDSGTQFTFLLGPVYTALRSHFLNRTNGILTVYEDPDFVFQGTMDLCYRISPVRIR 359

Query: 440 ---LPPLPAVTLVFRGAEMTVTGDRILYRVAGEIRGNDTIYCFTFGNSDLLGVEAYVIGH 499
              L  LP V+LVF GAE+ V+G  +LYRV     GND++YCFTFGNSDL+G+EAYVIGH
Sbjct: 360 SGILHRLPTVSLVFEGAEIAVSGQPLLYRVPHLTVGNDSVYCFTFGNSDLMGMEAYVIGH 419

Query: 500 LHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGMG 529
            HQQN+W+EFDL++SRIGLA + CD++GQ+LG+G
Sbjct: 420 HHQQNMWIEFDLQRSRIGLAPVECDVSGQRLGIG 452

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BLAST of Csor.00g201750 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 5.7e-39
Identity = 119/370 (32.16%), Postives = 186/370 (50.27%), Query Frame = 0


Query: 155 VSLTVGTPPQNVTMVIDTGSELSWLHCNR-SQNSSSSSSTFNPVQSSSYTPIPCSSSTCT 214
           ++L++GTP Q  + ++DTGS+L W  C   +Q  + S+  FNP  SSS++ +PCSS  C 
Sbjct: 97  MNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQLC- 156

Query: 215 DQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADMFYIGNSGIPNVVFGCMDSIFSS 274
            Q    P   +C SN  C  T  Y D S ++G++  +    G+  IPN+ FGC       
Sbjct: 157 -QALSSP---TC-SNNFCQYTYGYGDGSETQGSMGTETLTFGSVSIPNITFGC-----GE 216

Query: 275 NNE--EDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEY--DFPGLLLLGDANFSWLAPL 334
           NN+        GL+GM RG LS  SQ+   KFSYC++      P  LLLG    S  A  
Sbjct: 217 NNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGS 276

Query: 335 NYTPMIQISTPLPYFDRVAYTVQLQGIKVSNKLLPIPESVFE-PDHTGAGQTMVDSGTQF 394
             T +IQ S+ +P F    Y + L G+ V +  LPI  S F    + G G  ++DSGT  
Sbjct: 277 PNTTLIQ-SSQIPTF----YYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTL 336

Query: 395 TFLLGQAYTVLRDEFLNQTAGSLRVFEDPNFVFQGAMDLCYRVPTNHTRLPPLPAVTLVF 454
           T+ +  AY  +R EF++Q   +L V    +  F    DLC++ P++ + L  +P   + F
Sbjct: 337 TYFVNNAYQSVRQEFISQI--NLPVVNGSSSGF----DLCFQTPSDPSNL-QIPTFVMHF 396

Query: 455 RGAEMTVTGDRILYRVAGEIRGNDTIYCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKK 514
            G ++ +  +         I  ++ + C   G+S   G+   + G++ QQN+ + +D   
Sbjct: 397 DGGDLELPSENYF------ISPSNGLICLAMGSSS-QGMS--IFGNIQQQNMLVVYDTGN 434

Query: 515 SRIGLAEIRC 519
           S +  A  +C
Sbjct: 457 SVVSFASAQC 434

[Close]

BLAST of Csor.00g201750 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 2.4e-37
Identity = 106/367 (28.88%), Postives = 174/367 (47.41%), Query Frame = 0


Query: 155 VSLTVGTPPQNVTMVIDTGSELSWLHCNR-SQNSSSSSSTFNPVQSSSYTPIPCSSSTCT 214
           +++ +GTP  + + ++DTGS+L W  C   +Q  S  +  FNP  SSS++ +PC S  C 
Sbjct: 98  MNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQ 157

Query: 215 DQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADMFYIGNSGIPNVVFGCMDSIFSS 274
           D      +P+   +N  C  T  Y D S+++G +A + F    S +PN+ FGC +    +
Sbjct: 158 D------LPSETCNNNECQYTYGYGDGSTTQGYMATETFTFETSSVPNIAFGCGE---DN 217

Query: 275 NNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEY--DFPGLLLLGDANFSWLAPLNY 334
                    GL+GM  G LS  SQ+G  +FSYC++ Y    P  L LG A          
Sbjct: 218 QGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTLALGSAASGVPEGSPS 277

Query: 335 TPMIQISTPLPYFDRVAYTVQLQGIKVSNKLLPIPESVFEPDHTGAGQTMVDSGTQFTFL 394
           T +I  S    Y     Y + LQGI V    L IP S F+    G G  ++DSGT  T+L
Sbjct: 278 TTLIHSSLNPTY-----YYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYL 337

Query: 395 LGQAYTVLRDEFLNQTAGSLRVFEDPNFVFQGAMDLCYRVPTNHTRLPPLPAVTLVFRGA 454
              AY  +   F +Q   +L   ++ +      +  C++ P++ + +  +P +++ F G 
Sbjct: 338 PQDAYNAVAQAFTDQI--NLPTVDESS----SGLSTCFQQPSDGSTV-QVPEISMQFDGG 397

Query: 455 EMTVTGDRILYRVAGEIRGNDTIYCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKKSRI 514
            + +    IL   A      + + C   G+S  LG+   + G++ QQ   + +DL+   +
Sbjct: 398 VLNLGEQNILISPA------EGVICLAMGSSSQLGIS--IFGNIQQQETQVLYDLQNLAV 435

Query: 515 GLAEIRC 519
                +C
Sbjct: 458 SFVPTQC 435

[Close]

BLAST of Csor.00g201750 vs. ExPASy Swiss-Prot
Match: O04496 (Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 5.2e-32
Identity = 120/393 (30.53%), Postives = 180/393 (45.80%), Query Frame = 0


Query: 135 PESVP-RSPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSST 194
           P SVP  S ++L   H  +  V   +GTPPQ + MV+DT ++  WL C+     S++S++
Sbjct: 88  PTSVPVASGNQL---HIGNYVVRAKLGTPPQLMFMVLDTSNDAVWLPCSGCSGCSNASTS 147

Query: 195 FNPVQSSSYTPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADMFY 254
           FN   SS+Y+ + CS++ CT Q R    P+S     +C    SY   SS   +L  D   
Sbjct: 148 FNTNSSSTYSTVSCSTAQCT-QARGLTCPSSSPQPSVCSFNQSYGGDSSFSASLVQDTLT 207

Query: 255 IGNSGIPNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQ---MGFPKFSYCISEY 314
           +    IPN  FGC++S  +S N    +  GLMG+ RG +S VSQ   +    FSYC+  +
Sbjct: 208 LAPDVIPNFSFGCINS--ASGNSLPPQ--GLMGLGRGPMSLVSQTTSLYSGVFSYCLPSF 267

Query: 315 D---FPGLLLLGDANFSWLAPLNYTPMIQISTPLPYFDRVAYTVQLQGIKVSNKLLPIPE 374
               F G L LG         + YTP+++ +   P      Y V L G+ V +  +P+  
Sbjct: 268 RSFYFSGSLKLG--LLGQPKSIRYTPLLR-NPRRPSL----YYVNLTGVSVGSVQVPVDP 327

Query: 375 SVFEPDHTGAGQTMVDSGTQFTFLLGQAYTVLRDEFLNQTAGSLRVFEDPNFVFQGAMDL 434
                D      T++DSGT  T      Y  +RDEF  Q   S       +F   GA D 
Sbjct: 328 VYLTFDANSGAGTIIDSGTVITRFAQPVYEAIRDEFRKQVNVS-------SFSTLGAFDT 387

Query: 435 CYRVPTNHTRLPPLPAVTLVFRGAEMTVTGDRILYRVAGEIRGNDTIYCFTF-GNSDLLG 494
           C+     +      P +TL     ++ +  +  L   +       T+ C +  G      
Sbjct: 388 CFSADNENV----APKITLHMTSLDLKLPMENTLIHSSA-----GTLTCLSMAGIRQNAN 447

Query: 495 VEAYVIGHLHQQNVWMEFDLKKSRIGLAEIRCD 520
               VI +L QQN+ + FD+  SRIG+A   C+
Sbjct: 448 AVLNVIANLQQQNLRILFDVPNSRIGIAPEPCN 449

[Close]

BLAST of Csor.00g201750 vs. ExPASy Swiss-Prot
Match: Q6F4N5 (Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 2.2e-30
Identity = 120/385 (31.17%), Postives = 178/385 (46.23%), Query Frame = 0


Query: 152 SLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPVQSSSYTPIPCSSST 211
           S  V   +G+P Q + + +DT ++ +W HC+    +  SSS F P  SSSY  +PCSSS 
Sbjct: 78  SYVVRAGLGSPSQQLLLALDTSADATWAHCSPC-GTCPSSSLFAPANSSSYASLPCSSSW 137

Query: 212 C-TDQTRDFPIPASCDSNQLCHATL-------SYADASSSEGNLAADMFYIGNSGIPNVV 271
           C   Q +  P P          ATL        +ADA S +  LA+D   +G   IPN  
Sbjct: 138 CPLFQGQACPAPQGGGDAAPPPATLPTCAFSKPFADA-SFQAALASDTLRLGKDAIPNYT 197

Query: 272 FGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGF---PKFSYCISEYD---FPGLLL 331
           FGC+ S+  +    +    GL+G+ RG ++ +SQ G      FSYC+  Y    F G L 
Sbjct: 198 FGCVSSV--TGPTTNMPRQGLLGLGRGPMALLSQAGSLYNGVFSYCLPSYRSYYFSGSLR 257

Query: 332 LGDANFSWLAPLNYTPMIQISTPLPYFDRVAYTVQLQGIKVSNKLLPIPESVFEPD-HTG 391
           LG A       + YTPM++     P+   + Y V + G+ V +  + +P   F  D  TG
Sbjct: 258 LG-AGGGQPRSVRYTPMLR----NPHRSSL-YYVNVTGLSVGHAWVKVPAGSFAFDAATG 317

Query: 392 AGQTMVDSGTQFTFLLGQAYTVLRDEFLNQTAGSLRVFEDPNFVFQGAMDLCYRVPTNHT 451
           AG T+VDSGT  T      Y  LR+EF  Q A          +   GA D C+   T+  
Sbjct: 318 AG-TVVDSGTVITRWTAPVYAALREEFRRQVAA------PSGYTSLGAFDTCFN--TDEV 377

Query: 452 RLPPLPAVTL-VFRGAEMTVTGDRILYRVAGEIRGNDTIYCFTFGNS-DLLGVEAYVIGH 511
                PAVT+ +  G ++ +  +  L   +        + C     +   +     VI +
Sbjct: 378 AAGGAPAVTVHMDGGVDLALPMENTLIHSSA-----TPLACLAMAEAPQNVNSVVNVIAN 437

Query: 512 LHQQNVWMEFDLKKSRIGLAEIRCD 520
           L QQN+ + FD+  SR+G A+  C+
Sbjct: 438 LQQQNIRVVFDVANSRVGFAKESCN 438

[Close]

BLAST of Csor.00g201750 vs. NCBI nr
Match: KAG6596401.1 (Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1054 bits (2725), Expect = 0.0
Identity = 529/529 (100.00%), Postives = 529/529 (100.00%), Query Frame = 0


Query: 1   MASLCSFPRISSADPIKHLAAAPFPPSNHPIRPSALSLRQSSRNQKRTSTIVAAIGDVSA 60
           MASLCSFPRISSADPIKHLAAAPFPPSNHPIRPSALSLRQSSRNQKRTSTIVAAIGDVSA
Sbjct: 1   MASLCSFPRISSADPIKHLAAAPFPPSNHPIRPSALSLRQSSRNQKRTSTIVAAIGDVSA 60

Query: 61  DGTTYLIAGAVAVALVGTAFPILFSRKDLCPVCDGAGFVRKSGAALRANAARKDQAQIVC 120
           DGTTYLIAGAVAVALVGTAFPILFSRKDLCPVCDGAGFVRKSGAALRANAARKDQAQIVC
Sbjct: 61  DGTTYLIAGAVAVALVGTAFPILFSRKDLCPVCDGAGFVRKSGAALRANAARKDQAQIVC 120

Query: 121 SRCNGLGKLNQVIPPESVPRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLH 180
           SRCNGLGKLNQVIPPESVPRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLH
Sbjct: 121 SRCNGLGKLNQVIPPESVPRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLH 180

Query: 181 CNRSQNSSSSSSTFNPVQSSSYTPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADA 240
           CNRSQNSSSSSSTFNPVQSSSYTPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADA
Sbjct: 181 CNRSQNSSSSSSTFNPVQSSSYTPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADA 240

Query: 241 SSSEGNLAADMFYIGNSGIPNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGF 300
           SSSEGNLAADMFYIGNSGIPNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGF
Sbjct: 241 SSSEGNLAADMFYIGNSGIPNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGF 300

Query: 301 PKFSYCISEYDFPGLLLLGDANFSWLAPLNYTPMIQISTPLPYFDRVAYTVQLQGIKVSN 360
           PKFSYCISEYDFPGLLLLGDANFSWLAPLNYTPMIQISTPLPYFDRVAYTVQLQGIKVSN
Sbjct: 301 PKFSYCISEYDFPGLLLLGDANFSWLAPLNYTPMIQISTPLPYFDRVAYTVQLQGIKVSN 360

Query: 361 KLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGQAYTVLRDEFLNQTAGSLRVFEDPNFV 420
           KLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGQAYTVLRDEFLNQTAGSLRVFEDPNFV
Sbjct: 361 KLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGQAYTVLRDEFLNQTAGSLRVFEDPNFV 420

Query: 421 FQGAMDLCYRVPTNHTRLPPLPAVTLVFRGAEMTVTGDRILYRVAGEIRGNDTIYCFTFG 480
           FQGAMDLCYRVPTNHTRLPPLPAVTLVFRGAEMTVTGDRILYRVAGEIRGNDTIYCFTFG
Sbjct: 421 FQGAMDLCYRVPTNHTRLPPLPAVTLVFRGAEMTVTGDRILYRVAGEIRGNDTIYCFTFG 480

Query: 481 NSDLLGVEAYVIGHLHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 529
           NSDLLGVEAYVIGHLHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL
Sbjct: 481 NSDLLGVEAYVIGHLHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 529

[Close]

BLAST of Csor.00g201750 vs. NCBI nr
Match: KAG7027943.1 (Aspartic proteinase PCS1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1035 bits (2676), Expect = 0.0
Identity = 520/521 (99.81%), Postives = 520/521 (99.81%), Query Frame = 0


Query: 1   MASLCSFPRISSADPIKHLAAAPFPPSNHPIRPSALSLRQSSRNQKRTSTIVAAIGDVSA 60
           MASLCSFPRISSADPIKHLAAAPFPPSN PIRPSALSLRQSSRNQKRTSTIVAAIGDVSA
Sbjct: 1   MASLCSFPRISSADPIKHLAAAPFPPSNRPIRPSALSLRQSSRNQKRTSTIVAAIGDVSA 60

Query: 61  DGTTYLIAGAVAVALVGTAFPILFSRKDLCPVCDGAGFVRKSGAALRANAARKDQAQIVC 120
           DGTTYLIAGAVAVALVGTAFPILFSRKDLCPVCDGAGFVRKSGAALRANAARKDQAQIVC
Sbjct: 61  DGTTYLIAGAVAVALVGTAFPILFSRKDLCPVCDGAGFVRKSGAALRANAARKDQAQIVC 120

Query: 121 SRCNGLGKLNQVIPPESVPRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLH 180
           SRCNGLGKLNQVIPPESVPRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLH
Sbjct: 121 SRCNGLGKLNQVIPPESVPRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLH 180

Query: 181 CNRSQNSSSSSSTFNPVQSSSYTPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADA 240
           CNRSQNSSSSSSTFNPVQSSSYTPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADA
Sbjct: 181 CNRSQNSSSSSSTFNPVQSSSYTPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADA 240

Query: 241 SSSEGNLAADMFYIGNSGIPNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGF 300
           SSSEGNLAADMFYIGNSGIPNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGF
Sbjct: 241 SSSEGNLAADMFYIGNSGIPNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGF 300

Query: 301 PKFSYCISEYDFPGLLLLGDANFSWLAPLNYTPMIQISTPLPYFDRVAYTVQLQGIKVSN 360
           PKFSYCISEYDFPGLLLLGDANFSWLAPLNYTPMIQISTPLPYFDRVAYTVQLQGIKVSN
Sbjct: 301 PKFSYCISEYDFPGLLLLGDANFSWLAPLNYTPMIQISTPLPYFDRVAYTVQLQGIKVSN 360

Query: 361 KLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGQAYTVLRDEFLNQTAGSLRVFEDPNFV 420
           KLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGQAYTVLRDEFLNQTAGSLRVFEDPNFV
Sbjct: 361 KLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGQAYTVLRDEFLNQTAGSLRVFEDPNFV 420

Query: 421 FQGAMDLCYRVPTNHTRLPPLPAVTLVFRGAEMTVTGDRILYRVAGEIRGNDTIYCFTFG 480
           FQGAMDLCYRVPTNHTRLPPLPAVTLVFRGAEMTVTGDRILYRVAGEIRGNDTIYCFTFG
Sbjct: 421 FQGAMDLCYRVPTNHTRLPPLPAVTLVFRGAEMTVTGDRILYRVAGEIRGNDTIYCFTFG 480

Query: 481 NSDLLGVEAYVIGHLHQQNVWMEFDLKKSRIGLAEIRCDLA 521
           NSDLLGVEAYVIGHLHQQNVWMEFDLKKSRIGLAEIRCDLA
Sbjct: 481 NSDLLGVEAYVIGHLHQQNVWMEFDLKKSRIGLAEIRCDLA 521

[Close]

BLAST of Csor.00g201750 vs. NCBI nr
Match: XP_022934632.1 (uncharacterized protein LOC111441767 [Cucurbita moschata])

HSP 1 Score: 853 bits (2205), Expect = 2.01e-298
Identity = 456/600 (76.00%), Postives = 481/600 (80.17%), Query Frame = 0


Query: 1   MASLCSFPRISSADPIKHL-AAAPFPPSNHPIRPSALSLRQSSRNQKRTSTIVAAIGDVS 60
           MASLC+FPRISS +PIK   AAAPFPPSN P+RPSALSLRQSS   +R ST+VAAIGDVS
Sbjct: 36  MASLCTFPRISSTEPIKQTPAAAPFPPSNQPMRPSALSLRQSSSKHRRISTVVAAIGDVS 95

Query: 61  ADGTTYLIAGAVAVALVGTAFPILFSRKDLCP-VCDGAGFVRKSGAALRANAARKDQAQ- 120
           ADGTTYLIAGAVAVALVGTAFPILFSRKDL   +  G G + ++ A +      + Q + 
Sbjct: 96  ADGTTYLIAGAVAVALVGTAFPILFSRKDLWIFIFAGKGEIYRTVAKILGTLCSRKQKKD 155

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 156 NEVARISSLCGASNCPQLFSDSNIPGKKRKMRDYCIAFNSSNHKFLKSLFPFFLCTLFSV 215

Query: 181 ----IVCSRCNG---LGKLNQVIPPESVPRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVI 240
               I+CS  N    L    QVIPPES+ RSPDKLPFRHNVSLTVSLTVGTPPQNVTMVI
Sbjct: 216 FQNLILCSSQNPALLLPLKTQVIPPESIRRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVI 275

Query: 241 DTGSELSWLHCNRSQNSSSSSS-TFNPVQSSSYTPIPCSSSTCTDQTRDFPIPASCDSNQ 300
           DTGSELSWLHCNRSQNSSSSSS TFNP  SSSYTPIPCSSSTCTD+TRDFPIPASCDSN 
Sbjct: 276 DTGSELSWLHCNRSQNSSSSSSSTFNPAGSSSYTPIPCSSSTCTDRTRDFPIPASCDSNH 335

Query: 301 LCHATLSYADASSSEGNLAADMFYIGNSGIPNVVFGCMDSIFSSNNEEDSKNTGLMGMNR 360
           LCHATLSYADASSSEG LA D FYIGNSGI NVVFGCMDSIFSSNNEEDSKNTGLMGMNR
Sbjct: 336 LCHATLSYADASSSEGTLATDTFYIGNSGISNVVFGCMDSIFSSNNEEDSKNTGLMGMNR 395

Query: 361 GSLSFVSQMGFPKFSYCISEYDFPGLLLLGDANFSWLAPLNYTPMIQISTPLPYFDRVAY 420
           GSLSFVSQMGFPKFSYCISEYDF GLLLLGDANFSWLAPLNYTP+I++STPLPYFDRVAY
Sbjct: 396 GSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSWLAPLNYTPLIEMSTPLPYFDRVAY 455

Query: 421 TVQLQGIKVSNKLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGQAYTVLRDEFLNQTAG 480
           TVQL+GIKVS+KLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLG AYT LRDEFLN+TAG
Sbjct: 456 TVQLEGIKVSHKLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNRTAG 515

Query: 481 SLRVFEDPNFVFQGAMDLCYRVPTNHTRLPPLPAVTLVFRGAEMTVTGDRILYRVAGEIR 529
           S RVFED NFVFQGAMDLCYRVP N TRLPPLP+VTLVFRGAEMTVTGDRILYRV GEIR
Sbjct: 516 SFRVFEDSNFVFQGAMDLCYRVPMNQTRLPPLPSVTLVFRGAEMTVTGDRILYRVPGEIR 575

[Close]

BLAST of Csor.00g201750 vs. NCBI nr
Match: XP_022948372.1 (aspartic proteinase PCS1-like [Cucurbita moschata] >XP_022948381.1 aspartic proteinase PCS1-like [Cucurbita moschata])

HSP 1 Score: 796 bits (2055), Expect = 2.06e-287
Identity = 395/399 (99.00%), Postives = 395/399 (99.00%), Query Frame = 0


Query: 131 QVIPPESVPRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSS 190
           QVIPPESVPRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSS
Sbjct: 51  QVIPPESVPRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSS 110

Query: 191 SSTFNPVQSSSYTPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAAD 250
           SSTFNPVQSSSYTPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAAD
Sbjct: 111 SSTFNPVQSSSYTPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAAD 170

Query: 251 MFYIGNSGIPNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEY 310
            FYIGNSGIPNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEY
Sbjct: 171 TFYIGNSGIPNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEY 230

Query: 311 DFPGLLLLGDANFSWLAPLNYTPMIQISTPLPYFDRVAYTVQLQGIKVSNKLLPIPESVF 370
           DFPGLLLLGDANFSWLAPLNYTPMIQISTPLPYFDRVAYTVQLQGIKVSNKLLPIPES F
Sbjct: 231 DFPGLLLLGDANFSWLAPLNYTPMIQISTPLPYFDRVAYTVQLQGIKVSNKLLPIPESAF 290

Query: 371 EPDHTGAGQTMVDSGTQFTFLLGQAYTVLRDEFLNQTAGSLRVFEDPNFVFQGAMDLCYR 430
           EPDHTGAGQTMVDSGTQFTFLLGQAYTVLRDEFLNQTAGSLRV EDPNFVFQGAMDLCYR
Sbjct: 291 EPDHTGAGQTMVDSGTQFTFLLGQAYTVLRDEFLNQTAGSLRVSEDPNFVFQGAMDLCYR 350

Query: 431 VPTNHTRLPPLPAVTLVFRGAEMTVTGDRILYRVAGEIRGNDTIYCFTFGNSDLLGVEAY 490
           VPTN TRLPPLPAVTLVFRGAEMTVTGDRILYRVAGEIRGNDTIYCFTFGNSDLLGVEAY
Sbjct: 351 VPTNQTRLPPLPAVTLVFRGAEMTVTGDRILYRVAGEIRGNDTIYCFTFGNSDLLGVEAY 410

Query: 491 VIGHLHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 529
           VIGHLHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL
Sbjct: 411 VIGHLHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 449

[Close]

BLAST of Csor.00g201750 vs. NCBI nr
Match: XP_023540033.1 (aspartic proteinase PCS1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 790 bits (2041), Expect = 2.78e-285
Identity = 393/398 (98.74%), Postives = 393/398 (98.74%), Query Frame = 0


Query: 131 QVIPPESVPRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSS 190
           QVIPPESVPRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSS
Sbjct: 51  QVIPPESVPRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSS 110

Query: 191 SSTFNPVQSSSYTPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAAD 250
           SSTFN VQSSSYTPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAAD
Sbjct: 111 SSTFNTVQSSSYTPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAAD 170

Query: 251 MFYIGNSGIPNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEY 310
            F IGNSGIPNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEY
Sbjct: 171 TFNIGNSGIPNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEY 230

Query: 311 DFPGLLLLGDANFSWLAPLNYTPMIQISTPLPYFDRVAYTVQLQGIKVSNKLLPIPESVF 370
           DFPGLLLLGDANFSWLAPLNYTPMIQISTPLPYFDRVAYTVQLQGIKVSNKLLPIPESVF
Sbjct: 231 DFPGLLLLGDANFSWLAPLNYTPMIQISTPLPYFDRVAYTVQLQGIKVSNKLLPIPESVF 290

Query: 371 EPDHTGAGQTMVDSGTQFTFLLGQAYTVLRDEFLNQTAGSLRVFEDPNFVFQGAMDLCYR 430
           EPDHTGAGQTMVDSGTQFTFLLGQAYTVLRDEFLNQTAGSLRVFEDPNFVFQGAMDLCYR
Sbjct: 291 EPDHTGAGQTMVDSGTQFTFLLGQAYTVLRDEFLNQTAGSLRVFEDPNFVFQGAMDLCYR 350

Query: 431 VPTNHTRLPPLPAVTLVFRGAEMTVTGDRILYRVAGEIRGNDTIYCFTFGNSDLLGVEAY 490
           VPTN T LPPLPAVTLVFRGAEMTVTGDRILYRVAGEIRGNDTIYCFTFGNSDLLGVEAY
Sbjct: 351 VPTNQTHLPPLPAVTLVFRGAEMTVTGDRILYRVAGEIRGNDTIYCFTFGNSDLLGVEAY 410

Query: 491 VIGHLHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGMG 528
           VIGHLHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGMG
Sbjct: 411 VIGHLHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGMG 448

[Close]

BLAST of Csor.00g201750 vs. ExPASy TrEMBL
Match: A0A6J1F3C7 (Laccase OS=Cucurbita moschata OX=3662 GN=LOC111441767 PE=3 SV=1)

HSP 1 Score: 853 bits (2205), Expect = 9.74e-299
Identity = 456/600 (76.00%), Postives = 481/600 (80.17%), Query Frame = 0


Query: 1   MASLCSFPRISSADPIKHL-AAAPFPPSNHPIRPSALSLRQSSRNQKRTSTIVAAIGDVS 60
           MASLC+FPRISS +PIK   AAAPFPPSN P+RPSALSLRQSS   +R ST+VAAIGDVS
Sbjct: 36  MASLCTFPRISSTEPIKQTPAAAPFPPSNQPMRPSALSLRQSSSKHRRISTVVAAIGDVS 95

Query: 61  ADGTTYLIAGAVAVALVGTAFPILFSRKDLCP-VCDGAGFVRKSGAALRANAARKDQAQ- 120
           ADGTTYLIAGAVAVALVGTAFPILFSRKDL   +  G G + ++ A +      + Q + 
Sbjct: 96  ADGTTYLIAGAVAVALVGTAFPILFSRKDLWIFIFAGKGEIYRTVAKILGTLCSRKQKKD 155

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 156 NEVARISSLCGASNCPQLFSDSNIPGKKRKMRDYCIAFNSSNHKFLKSLFPFFLCTLFSV 215

Query: 181 ----IVCSRCNG---LGKLNQVIPPESVPRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVI 240
               I+CS  N    L    QVIPPES+ RSPDKLPFRHNVSLTVSLTVGTPPQNVTMVI
Sbjct: 216 FQNLILCSSQNPALLLPLKTQVIPPESIRRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVI 275

Query: 241 DTGSELSWLHCNRSQNSSSSSS-TFNPVQSSSYTPIPCSSSTCTDQTRDFPIPASCDSNQ 300
           DTGSELSWLHCNRSQNSSSSSS TFNP  SSSYTPIPCSSSTCTD+TRDFPIPASCDSN 
Sbjct: 276 DTGSELSWLHCNRSQNSSSSSSSTFNPAGSSSYTPIPCSSSTCTDRTRDFPIPASCDSNH 335

Query: 301 LCHATLSYADASSSEGNLAADMFYIGNSGIPNVVFGCMDSIFSSNNEEDSKNTGLMGMNR 360
           LCHATLSYADASSSEG LA D FYIGNSGI NVVFGCMDSIFSSNNEEDSKNTGLMGMNR
Sbjct: 336 LCHATLSYADASSSEGTLATDTFYIGNSGISNVVFGCMDSIFSSNNEEDSKNTGLMGMNR 395

Query: 361 GSLSFVSQMGFPKFSYCISEYDFPGLLLLGDANFSWLAPLNYTPMIQISTPLPYFDRVAY 420
           GSLSFVSQMGFPKFSYCISEYDF GLLLLGDANFSWLAPLNYTP+I++STPLPYFDRVAY
Sbjct: 396 GSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSWLAPLNYTPLIEMSTPLPYFDRVAY 455

Query: 421 TVQLQGIKVSNKLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGQAYTVLRDEFLNQTAG 480
           TVQL+GIKVS+KLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLG AYT LRDEFLN+TAG
Sbjct: 456 TVQLEGIKVSHKLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNRTAG 515

Query: 481 SLRVFEDPNFVFQGAMDLCYRVPTNHTRLPPLPAVTLVFRGAEMTVTGDRILYRVAGEIR 529
           S RVFED NFVFQGAMDLCYRVP N TRLPPLP+VTLVFRGAEMTVTGDRILYRV GEIR
Sbjct: 516 SFRVFEDSNFVFQGAMDLCYRVPMNQTRLPPLPSVTLVFRGAEMTVTGDRILYRVPGEIR 575

[Close]

BLAST of Csor.00g201750 vs. ExPASy TrEMBL
Match: A0A6J1G928 (aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111452070 PE=3 SV=1)

HSP 1 Score: 796 bits (2055), Expect = 9.95e-288
Identity = 395/399 (99.00%), Postives = 395/399 (99.00%), Query Frame = 0


Query: 131 QVIPPESVPRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSS 190
           QVIPPESVPRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSS
Sbjct: 51  QVIPPESVPRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSS 110

Query: 191 SSTFNPVQSSSYTPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAAD 250
           SSTFNPVQSSSYTPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAAD
Sbjct: 111 SSTFNPVQSSSYTPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAAD 170

Query: 251 MFYIGNSGIPNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEY 310
            FYIGNSGIPNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEY
Sbjct: 171 TFYIGNSGIPNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEY 230

Query: 311 DFPGLLLLGDANFSWLAPLNYTPMIQISTPLPYFDRVAYTVQLQGIKVSNKLLPIPESVF 370
           DFPGLLLLGDANFSWLAPLNYTPMIQISTPLPYFDRVAYTVQLQGIKVSNKLLPIPES F
Sbjct: 231 DFPGLLLLGDANFSWLAPLNYTPMIQISTPLPYFDRVAYTVQLQGIKVSNKLLPIPESAF 290

Query: 371 EPDHTGAGQTMVDSGTQFTFLLGQAYTVLRDEFLNQTAGSLRVFEDPNFVFQGAMDLCYR 430
           EPDHTGAGQTMVDSGTQFTFLLGQAYTVLRDEFLNQTAGSLRV EDPNFVFQGAMDLCYR
Sbjct: 291 EPDHTGAGQTMVDSGTQFTFLLGQAYTVLRDEFLNQTAGSLRVSEDPNFVFQGAMDLCYR 350

Query: 431 VPTNHTRLPPLPAVTLVFRGAEMTVTGDRILYRVAGEIRGNDTIYCFTFGNSDLLGVEAY 490
           VPTN TRLPPLPAVTLVFRGAEMTVTGDRILYRVAGEIRGNDTIYCFTFGNSDLLGVEAY
Sbjct: 351 VPTNQTRLPPLPAVTLVFRGAEMTVTGDRILYRVAGEIRGNDTIYCFTFGNSDLLGVEAY 410

Query: 491 VIGHLHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 529
           VIGHLHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL
Sbjct: 411 VIGHLHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 449

[Close]

BLAST of Csor.00g201750 vs. ExPASy TrEMBL
Match: A0A6J1I7C0 (aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111470272 PE=3 SV=1)

HSP 1 Score: 787 bits (2033), Expect = 2.23e-284
Identity = 391/399 (97.99%), Postives = 393/399 (98.50%), Query Frame = 0


Query: 131 QVIPPESVPRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSS 190
           QVIPPESV RSPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSS
Sbjct: 51  QVIPPESVWRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSS 110

Query: 191 SSTFNPVQSSSYTPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAAD 250
           SSTFNPVQSSSYTPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAAD
Sbjct: 111 SSTFNPVQSSSYTPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAAD 170

Query: 251 MFYIGNSGIPNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEY 310
            FYIGNS IPNVVFGCMDSIFSSNN EDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEY
Sbjct: 171 TFYIGNSSIPNVVFGCMDSIFSSNNVEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEY 230

Query: 311 DFPGLLLLGDANFSWLAPLNYTPMIQISTPLPYFDRVAYTVQLQGIKVSNKLLPIPESVF 370
           DFPGLLLLGDANFSWLAPLNYTPMIQISTPLPYFDRVAYTVQLQGIKVSNKLLPIPESVF
Sbjct: 231 DFPGLLLLGDANFSWLAPLNYTPMIQISTPLPYFDRVAYTVQLQGIKVSNKLLPIPESVF 290

Query: 371 EPDHTGAGQTMVDSGTQFTFLLGQAYTVLRDEFLNQTAGSLRVFEDPNFVFQGAMDLCYR 430
           EPDHTGAGQTMVDSGTQFTFLLGQAYTVLRDEFLNQTAGSLRVFEDPNFVFQGAMDLCYR
Sbjct: 291 EPDHTGAGQTMVDSGTQFTFLLGQAYTVLRDEFLNQTAGSLRVFEDPNFVFQGAMDLCYR 350

Query: 431 VPTNHTRLPPLPAVTLVFRGAEMTVTGDRILYRVAGEIRGNDTIYCFTFGNSDLLGVEAY 490
           VPTN TRLPPLPAVTLVFRGAEMTVTGDRILY+VAGEIRGNDTIYCFTFGN+DLLGVEAY
Sbjct: 351 VPTNQTRLPPLPAVTLVFRGAEMTVTGDRILYKVAGEIRGNDTIYCFTFGNADLLGVEAY 410

Query: 491 VIGHLHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 529
           VIGHLHQQNVWMEFDLKKSRIGL EIRCDLAGQKLGMGL
Sbjct: 411 VIGHLHQQNVWMEFDLKKSRIGLVEIRCDLAGQKLGMGL 449

[Close]

BLAST of Csor.00g201750 vs. ExPASy TrEMBL
Match: A0A6J1J2F5 (aspartic proteinase PCS1 OS=Cucurbita maxima OX=3661 GN=LOC111482077 PE=3 SV=1)

HSP 1 Score: 756 bits (1952), Expect = 4.76e-272
Identity = 378/418 (90.43%), Postives = 391/418 (93.54%), Query Frame = 0


Query: 115 QAQIVCSRCNG---LGKLNQVIPPESVPRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVID 174
           Q  I+CS  N    L    QVIPPES+ RSPDKLPFRHNVSLTVSLTVGTPPQNVTMVID
Sbjct: 32  QNLILCSSLNPALLLPLKTQVIPPESIRRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVID 91

Query: 175 TGSELSWLHCNRSQNSSSSSSTFNPVQSSSYTPIPCSSSTCTDQTRDFPIPASCDSNQLC 234
           TGSELSWLHCNRSQNSSSSSSTFNP  SSSYTPIPCSSSTCTDQTRDFPIPASCDSN LC
Sbjct: 92  TGSELSWLHCNRSQNSSSSSSTFNPAGSSSYTPIPCSSSTCTDQTRDFPIPASCDSNHLC 151

Query: 235 HATLSYADASSSEGNLAADMFYIGNSGIPNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGS 294
           HATLSYADASSSEG LA D FYIGNSGI NVVFGCMDSIFSSNNEEDSKNTGLMGMNRGS
Sbjct: 152 HATLSYADASSSEGTLATDTFYIGNSGISNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGS 211

Query: 295 LSFVSQMGFPKFSYCISEYDFPGLLLLGDANFSWLAPLNYTPMIQISTPLPYFDRVAYTV 354
           LSFVSQMGFPKFSYCISEYDF GLLLLGDANFSWLAPLNYTP+I++STPLPYFDRVAYTV
Sbjct: 212 LSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSWLAPLNYTPLIEMSTPLPYFDRVAYTV 271

Query: 355 QLQGIKVSNKLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGQAYTVLRDEFLNQTAGSL 414
           QL+GIKVS+KLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLG AYT LRDEFLN+TAGS+
Sbjct: 272 QLEGIKVSHKLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNRTAGSI 331

Query: 415 RVFEDPNFVFQGAMDLCYRVPTNHTRLPPLPAVTLVFRGAEMTVTGDRILYRVAGEIRGN 474
           RVFED NFVFQGAMDLCYRVP N TRLPPLP+VTLVFRGAEMTVTGDRILYRV GEIRGN
Sbjct: 332 RVFEDSNFVFQGAMDLCYRVPMNQTRLPPLPSVTLVFRGAEMTVTGDRILYRVPGEIRGN 391

Query: 475 DTIYCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 529
           D+I+CFTFGNSDLLGVEA+VIGHLHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL
Sbjct: 392 DSIHCFTFGNSDLLGVEAFVIGHLHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 449

[Close]

BLAST of Csor.00g201750 vs. ExPASy TrEMBL
Match: A0A6J1F9C1 (aspartic proteinase PCS1 OS=Cucurbita moschata OX=3662 GN=LOC111442007 PE=3 SV=1)

HSP 1 Score: 748 bits (1932), Expect = 5.46e-269
Identity = 377/419 (89.98%), Postives = 390/419 (93.08%), Query Frame = 0


Query: 115 QAQIVCSRCNG---LGKLNQVIPPESVPRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVID 174
           Q  I+CS  N    L    QVIPPES+ RSPDKLPFRHNVSLTVSLTVGTPPQNVTMVID
Sbjct: 32  QNLILCSSQNPALLLPLKTQVIPPESIRRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVID 91

Query: 175 TGSELSWLHCNRSQNSSSSSS-TFNPVQSSSYTPIPCSSSTCTDQTRDFPIPASCDSNQL 234
           TGSELSWLHCNRSQNSSSSSS TFNP  SSSYTPIPCSSSTCTD+TRDFPIPASCDSN L
Sbjct: 92  TGSELSWLHCNRSQNSSSSSSSTFNPAGSSSYTPIPCSSSTCTDRTRDFPIPASCDSNHL 151

Query: 235 CHATLSYADASSSEGNLAADMFYIGNSGIPNVVFGCMDSIFSSNNEEDSKNTGLMGMNRG 294
           CHATLSYADASSSEG LA D FYIGNSGI NVVFGCMDSIFSSNNEEDSKNTGLMGMNRG
Sbjct: 152 CHATLSYADASSSEGTLATDTFYIGNSGISNVVFGCMDSIFSSNNEEDSKNTGLMGMNRG 211

Query: 295 SLSFVSQMGFPKFSYCISEYDFPGLLLLGDANFSWLAPLNYTPMIQISTPLPYFDRVAYT 354
           SLSFVSQMGFPKFSYCISEYDF GLLLLGDANFSWLAPLNYTP+I++STPLPYFDRVAYT
Sbjct: 212 SLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSWLAPLNYTPLIEMSTPLPYFDRVAYT 271

Query: 355 VQLQGIKVSNKLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGQAYTVLRDEFLNQTAGS 414
           VQL+GIKVS+KLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLG AYT LRDEFLN+TAGS
Sbjct: 272 VQLEGIKVSHKLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNRTAGS 331

Query: 415 LRVFEDPNFVFQGAMDLCYRVPTNHTRLPPLPAVTLVFRGAEMTVTGDRILYRVAGEIRG 474
            RVFED NFVFQGAMDLCYRVP N TRLPPLP+VTLVFRGAEMTVTGDRILYRV GEIRG
Sbjct: 332 FRVFEDSNFVFQGAMDLCYRVPMNQTRLPPLPSVTLVFRGAEMTVTGDRILYRVPGEIRG 391

Query: 475 NDTIYCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 529
           ND+I+CFTFGNSDLLGVEA+VIGHLHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL
Sbjct: 392 NDSIHCFTFGNSDLLGVEAFVIGHLHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 450

[Close]

BLAST of Csor.00g201750 vs. TAIR 10
Match: AT5G02190.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 564.3 bits (1453), Expect = 1.0e-160
Identity = 278/394 (70.56%), Postives = 328/394 (83.25%), Query Frame = 0


Query: 140 RSPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPVQS 199
           R  DKL F HNV+LTV+LTVGTPPQN++MVIDTGSELSWL CNRS N +  ++ F+P +S
Sbjct: 60  RPTDKLHFHHNVTLTVTLTVGTPPQNISMVIDTGSELSWLRCNRSSNPNPVNN-FDPTRS 119

Query: 200 SSYTPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADMFYIGNS-G 259
           SSY+PIPCSS TC  +TRDF IPASCDS++LCHATLSYADASSSEGNLAA++F+ GNS  
Sbjct: 120 SSYSPIPCSSPTCRTRTRDFLIPASCDSDKLCHATLSYADASSSEGNLAAEIFHFGNSTN 179

Query: 260 IPNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCIS-EYDFPGLLL 319
             N++FGCM S+  S+ EED+K TGL+GMNRGSLSF+SQMGFPKFSYCIS   DFPG LL
Sbjct: 180 DSNLIFGCMGSVSGSDPEEDTKTTGLLGMNRGSLSFISQMGFPKFSYCISGTDDFPGFLL 239

Query: 320 LGDANFSWLAPLNYTPMIQISTPLPYFDRVAYTVQLQGIKVSNKLLPIPESVFEPDHTGA 379
           LGD+NF+WL PLNYTP+I+ISTPLPYFDRVAYTVQL GIKV+ KLLPIP+SV  PDHTGA
Sbjct: 240 LGDSNFTWLTPLNYTPLIRISTPLPYFDRVAYTVQLTGIKVNGKLLPIPKSVLVPDHTGA 299

Query: 380 GQTMVDSGTQFTFLLGQAYTVLRDEFLNQTAGSLRVFEDPNFVFQGAMDLCYRVPTNHTR 439
           GQTMVDSGTQFTFLLG  YT LR  FLN+T G L V+EDP+FVFQG MDLCYR+     R
Sbjct: 300 GQTMVDSGTQFTFLLGPVYTALRSHFLNRTNGILTVYEDPDFVFQGTMDLCYRISPVRIR 359

Query: 440 ---LPPLPAVTLVFRGAEMTVTGDRILYRVAGEIRGNDTIYCFTFGNSDLLGVEAYVIGH 499
              L  LP V+LVF GAE+ V+G  +LYRV     GND++YCFTFGNSDL+G+EAYVIGH
Sbjct: 360 SGILHRLPTVSLVFEGAEIAVSGQPLLYRVPHLTVGNDSVYCFTFGNSDLMGMEAYVIGH 419

Query: 500 LHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGMG 529
            HQQN+W+EFDL++SRIGLA + CD++GQ+LG+G
Sbjct: 420 HHQQNMWIEFDLQRSRIGLAPVECDVSGQRLGIG 452

[Close]

BLAST of Csor.00g201750 vs. TAIR 10
Match: AT2G39710.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 526.6 bits (1355), Expect = 2.4e-149
Identity = 257/391 (65.73%), Postives = 310/391 (79.28%), Query Frame = 0


Query: 141 SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPVQSS 200
           S DKL FRHNV+LTV+L VG PPQN++MV+DTGSELSWLHC +S N     S FNPV SS
Sbjct: 53  SSDKLSFRHNVTLTVTLAVGDPPQNISMVLDTGSELSWLHCKKSPN---LGSVFNPVSSS 112

Query: 201 SYTPIPCSSSTCTDQTRDFPIPASCD-SNQLCHATLSYADASSSEGNLAADMFYIGNSGI 260
           +Y+P+PCSS  C  +TRD PIPASCD    LCH  +SYADA+S EGNLA + F IG+   
Sbjct: 113 TYSPVPCSSPICRTRTRDLPIPASCDPKTHLCHVAISYADATSIEGNLAHETFVIGSVTR 172

Query: 261 PNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFPGLLLLG 320
           P  +FGCMDS  SSN+EED+K+TGLMGMNRGSLSFV+Q+GF KFSYCIS  D  G LLLG
Sbjct: 173 PGTLFGCMDSGLSSNSEEDAKSTGLMGMNRGSLSFVNQLGFSKFSYCISGSDSSGFLLLG 232

Query: 321 DANFSWLAPLNYTPMIQISTPLPYFDRVAYTVQLQGIKVSNKLLPIPESVFEPDHTGAGQ 380
           DA++SWL P+ YTP++  STPLPYFDRVAYTVQL+GI+V +K+L +P+SVF PDHTGAGQ
Sbjct: 233 DASYSWLGPIQYTPLVLQSTPLPYFDRVAYTVQLEGIRVGSKILSLPKSVFVPDHTGAGQ 292

Query: 381 TMVDSGTQFTFLLGQAYTVLRDEFLNQTAGSLRVFEDPNFVFQGAMDLCYRV-PTNHTRL 440
           TMVDSGTQFTFL+G  YT L++EF+ QT   LR+ +DP+FVFQG MDLCY+V  T     
Sbjct: 293 TMVDSGTQFTFLMGPVYTALKNEFITQTKSVLRLVDDPDFVFQGTMDLCYKVGSTTRPNF 352

Query: 441 PPLPAVTLVFRGAEMTVTGDRILYRVAGE-IRGNDTIYCFTFGNSDLLGVEAYVIGHLHQ 500
             LP V+L+FRGAEM+V+G ++LYRV G    G + +YCFTFGNSDLLG+EA+VIGH HQ
Sbjct: 353 SGLPMVSLMFRGAEMSVSGQKLLYRVNGAGSEGKEEVYCFTFGNSDLLGIEAFVIGHHHQ 412

Query: 501 QNVWMEFDLKKSRIGLA-EIRCDLAGQKLGM 528
           QNVWMEFDL KSR+G A  +RCDLA Q+LG+
Sbjct: 413 QNVWMEFDLAKSRVGFAGNVRCDLASQRLGL 440

[Close]

BLAST of Csor.00g201750 vs. TAIR 10
Match: AT1G66180.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 254.2 bits (648), Expect = 2.3e-67
Identity = 152/403 (37.72%), Postives = 223/403 (55.33%), Query Frame = 0


Query: 129 LNQVIPPESVPRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSS 188
           L++  P  S P    +  F+++++L +SL +GTPPQ   MV+DTGS+LSW+ C+R +   
Sbjct: 48  LSRKNPSPSSPPYNFRSRFKYSMALIISLPIGTPPQAQQMVLDTGSQLSWIQCHRKKLPP 107

Query: 189 SSSSTFNPVQSSSYTPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLA 248
              ++F+P  SSS++ +PCS   C  +  DF +P SCDSN+LCH +  YAD + +EGNL 
Sbjct: 108 KPKTSFDPSLSSSFSTLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLV 167

Query: 249 ADMFYIGNSGI-PNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCI 308
            +     N+ I P ++ GC          E S + G++GMNRG LSFVSQ    KFSYCI
Sbjct: 168 KEKITFSNTEITPPLILGCA--------TESSDDRGILGMNRGRLSFVSQAKISKFSYCI 227

Query: 309 SE------YDFPGLLLLGD----ANFSWLAPLNYTPMIQISTPLPYFDRVAYTVQLQGIK 368
                   +   G   LGD      F +++ L +      S  +P  D +AYTV + GI+
Sbjct: 228 PPKSNRPGFTPTGSFYLGDNPNSHGFKYVSLLTFPE----SQRMPNLDPLAYTVPMIGIR 287

Query: 369 VSNKLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGQAYTVLRDEFLNQTAGSLRVFEDP 428
              K L I  SVF PD  G+GQTMVDSG++FT L+  AY  +R E + +    L+     
Sbjct: 288 FGLKKLNISGSVFRPDAGGSGQTMVDSGSEFTHLVDAAYDKVRAEIMTRVGRRLK----K 347

Query: 429 NFVFQGAMDLCYRVPTNHTRLPPL--PAVTLVFRGAEMTVTGDRILYRVAGEIRGNDTIY 488
            +V+ G  D+C+    N   +P L    V +  RG E+ V  +R+L  V G       I+
Sbjct: 348 GYVYGGTADMCF--DGNVAMIPRLIGDLVFVFTRGVEILVPKERVLVNVGG------GIH 407

Query: 489 CFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKKSRIGLAEIRC 519
           C   G S +LG  + +IG++HQQN+W+EFD+   R+G A+  C
Sbjct: 408 CVGIGRSSMLGAASNIIGNVHQQNLWVEFDVTNRRVGFAKADC 426

[Close]

BLAST of Csor.00g201750 vs. TAIR 10
Match: AT5G37540.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 241.9 bits (616), Expect = 1.2e-63
Identity = 152/405 (37.53%), Postives = 222/405 (54.81%), Query Frame = 0


Query: 137 SVPRSPDKLPFRHNV----SLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSS---S 196
           S P SP    FR N+    +L +SL +GTP Q+  +V+DTGS+LSW+ C+  +       
Sbjct: 62  SPPSSP--YTFRSNIKYSMALILSLPIGTPSQSQELVLDTGSQLSWIQCHPKKIKKPLPP 121

Query: 197 SSSTFNPVQSSSYTPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAA 256
            +++F+P  SSS++ +PCS   C  +  DF +P SCDSN+LCH +  YAD + +EGNL  
Sbjct: 122 PTTSFDPSLSSSFSDLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVK 181

Query: 257 DMFYIGNS-GIPNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCI- 316
           + F   NS   P ++ GC         +E +   G++GMN G LSF+SQ    KFSYCI 
Sbjct: 182 EKFTFSNSQTTPPLILGCA--------KESTDEKGILGMNLGRLSFISQAKISKFSYCIP 241

Query: 317 SEYDFPGL-----LLLGD----ANFSWLAPLNYTPMIQISTPLPYFDRVAYTVQLQGIKV 376
           +  + PGL       LGD      F +++ L +      S  +P  D +AYTV LQGI++
Sbjct: 242 TRSNRPGLASTGSFYLGDNPNSRGFKYVSLLTFPQ----SQRMPNLDPLAYTVPLQGIRI 301

Query: 377 SNKLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGQAYTVLRDEFLNQTAGSLRVFEDPN 436
             K L IP SVF PD  G+GQTMVDSG++FT L+  AY  +++E +      L+      
Sbjct: 302 GQKRLNIPGSVFRPDAGGSGQTMVDSGSEFTHLVDVAYDKVKEEIVRLVGSRLK----KG 361

Query: 437 FVFQGAMDLCYRVPTNHTRLPPLPAVTLVF---RGAEMTVTGDRILYRVAGEIRGNDTIY 496
           +V+    D+C+    NH+         LVF   RG E+ V    +L  V G       I+
Sbjct: 362 YVYGSTADMCF--DGNHSMEIGRLIGDLVFEFGRGVEILVEKQSLLVNVGG------GIH 421

Query: 497 CFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKKSRIGLAEIRCDL 521
           C   G S +LG  + +IG++HQQN+W+EFD+   R+G ++  C L
Sbjct: 422 CVGIGRSSMLGAASNIIGNVHQQNLWVEFDVTNRRVGFSKAECRL 440

[Close]

BLAST of Csor.00g201750 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 141.7 bits (356), Expect = 1.6e-33
Identity = 113/380 (29.74%), Postives = 186/380 (48.95%), Query Frame = 0


Query: 155 VSLTVGTPPQNVTMVIDTGSELSWLHCNR-SQNSSSSSSTFNPVQSSSYTPIPCSSSTCT 214
           + L++G P    + ++DTGS+L W  C   ++     +  F+P +SSSY+ + CSS  C 
Sbjct: 109 MELSIGNPAVKYSAIVDTGSDLIWTQCKPCTECFDQPTPIFDPEKSSSYSKVGCSSGLCN 168

Query: 215 DQTRDFPIPASCDSNQ-LCHATLSYADASSSEGNLAADMF-YIGNSGIPNVVFGCMDSIF 274
              R     ++C+ ++  C    +Y D SS+ G LA + F +   + I  + FGC     
Sbjct: 169 ALPR-----SNCNEDKDACEYLYTYGDYSSTRGLLATETFTFEDENSISGIGFGC----- 228

Query: 275 SSNNEED--SKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYD--------FPGLLLLGDA 334
              NE D  S+ +GL+G+ RG LS +SQ+   KFSYC++  +        F G L  G  
Sbjct: 229 GVENEGDGFSQGSGLVGLGRGPLSLISQLKETKFSYCLTSIEDSEASSSLFIGSLASGIV 288

Query: 335 N---FSWLAPLNYTPMIQISTPLPYFDRVAYTVQLQGIKVSNKLLPIPESVFEPDHTGAG 394
           N    S    +  T  +  +   P F    Y ++LQGI V  K L + +S FE    G G
Sbjct: 289 NKTGASLDGEVTKTMSLLRNPDQPSF----YYLELQGITVGAKRLSVEKSTFELAEDGTG 348

Query: 395 QTMVDSGTQFTFLLGQAYTVLRDEFLNQTAGSLRVFEDPNFVFQGAMDLCYRVPTNHTRL 454
             ++DSGT  T+L   A+ VL++EF ++   SL V +  +      +DLC+++P     +
Sbjct: 349 GMIIDSGTTITYLEETAFKVLKEEFTSRM--SLPVDDSGS----TGLDLCFKLPDAAKNI 408

Query: 455 PPLPAVTLVFRGAEMTVTGDRILYRVAGEIRGNDTIYCFTFGNSDLLGVEAYVIGHLHQQ 514
             +P +   F+GA++ + G+   Y VA    G   + C   G+S+ +     + G++ QQ
Sbjct: 409 -AVPKMIFHFKGADLELPGEN--YMVADSSTG---VLCLAMGSSNGMS----IFGNVQQQ 458

Query: 515 NVWMEFDLKKSRIGLAEIRC 519
           N  +  DL+K  +      C
Sbjct: 469 NFNVLHDLEKETVSFVPTEC 458

The following BLAST results are available for this feature:

BLAST of Csor.00g201750 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LZL3	1.4e-159	70.56	Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1	[more]
Q766C3	5.7e-39	32.16	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q766C2	2.4e-37	28.88	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
O04496	5.2e-32	30.53	Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1	[more]
Q6F4N5	2.2e-30	31.17	Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1	[more]

BLAST of Csor.00g201750 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6596401.1	0.0	100.00	Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7027943.1	0.0	99.81	Aspartic proteinase PCS1 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022934632.1	2.01e-298	76.00	uncharacterized protein LOC111441767 [Cucurbita moschata]	[more]
XP_022948372.1	2.06e-287	99.00	aspartic proteinase PCS1-like [Cucurbita moschata] >XP_022948381.1 aspartic prot...	[more]
XP_023540033.1	2.78e-285	98.74	aspartic proteinase PCS1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Csor.00g201750 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F3C7	9.74e-299	76.00	Laccase OS=Cucurbita moschata OX=3662 GN=LOC111441767 PE=3 SV=1	[more]
A0A6J1G928	9.95e-288	99.00	aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111452070 PE=3...	[more]
A0A6J1I7C0	2.23e-284	97.99	aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111470272 PE=3 S...	[more]
A0A6J1J2F5	4.76e-272	90.43	aspartic proteinase PCS1 OS=Cucurbita maxima OX=3661 GN=LOC111482077 PE=3 SV=1	[more]
A0A6J1F9C1	5.46e-269	89.98	aspartic proteinase PCS1 OS=Cucurbita moschata OX=3662 GN=LOC111442007 PE=3 SV=1	[more]

BLAST of Csor.00g201750 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G02190.1	1.0e-160	70.56	Eukaryotic aspartyl protease family protein	[more]
AT2G39710.1	2.4e-149	65.73	Eukaryotic aspartyl protease family protein	[more]
AT1G66180.1	2.3e-67	37.72	Eukaryotic aspartyl protease family protein	[more]
AT5G37540.1	1.2e-63	37.53	Eukaryotic aspartyl protease family protein	[more]
AT2G03200.1	1.6e-33	29.74	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 159..179 score: 46.19 coord: 380..391 score: 32.91 coord: 490..505 score: 18.07
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR47965	ASPARTYL PROTEASE-RELATED	coord: 135..527
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 146..320 e-value: 3.7E-36 score: 126.8
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 324..521 e-value: 2.0E-43 score: 150.1
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 154..518
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 348..513 e-value: 3.5E-36 score: 124.4
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 155..320 e-value: 6.9E-39 score: 133.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 24..43
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 187..207
None	No IPR available	PANTHER	PTHR47965:SF64	ASPARTIC PROTEINASE PCS1	coord: 135..527
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 168..179
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 153..514 score: 35.118797
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 154..518 e-value: 1.72559E-70 score: 224.449

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g201750.m01	Csor.00g201750.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046274	lignin catabolic process
biological_process	GO:0012501	programmed cell death
biological_process	GO:0006508	proteolysis
cellular_component	GO:0048046	apoplast
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004190	aspartic-type endopeptidase activity
molecular_function	GO:0005507	copper ion binding
molecular_function	GO:0052716	hydroquinone:oxygen oxidoreductase activity