Csor.00g200290 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g200290
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionDol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase
LocationCsor_Chr03: 4988718 .. 4989133 (-)
RNA-Seq ExpressionCsor.00g200290
SyntenyCsor.00g200290
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTAAGTTTTTCGTCATATTCGTTTCGATTTTTTTAAATTTGGAGTTTGTTCGTTCTCAATTGCATTTTCTTAGTAAAATTACCTGAAATATTGATCCTGACTGATGTTTAAGGACGAGATATTTCATGTACCTCAAGCTCAGAAATGCTGCAGTGGGAACTTCCGAAGTTGGGACCCGAAGATTTCAAATCCTCCTGGACTGTACGCTCTTATCTCTCTATTCTATGATTCTTTCAACTAGAAGTGTTGGCTCCTTTTCTGGATTATTGTCCTGATTTGTATCCTTATTCAGCTTTCACTTGCCCACGTTGCTTCTTTGTTTCCTGCCATTGTCTTTACTCAAGCCGTACCATCATTTTCTGAAGCTTGTTCCATTCATATTCTCCGTTCAACTAATTTGCATCATTTTCTAG

mRNA sequence

ATGGACGAGATATTTCATGTACCTCAAGCTCAGAAATGCTGCAGTGGGAACTTCCGAAGTTGGGACCCGAAGATTTCAAATCCTCCTGGACTCTTTCACTTGCCCACGTTGCTTCTTTGTTTCCTGCCATTGTCTTTACTCAAGCCGTACCATCATTTTCTGAAGCTTGTTCCATTCATATTCTCCGTTCAACTAATTTGCATCATTTTCTAG

Coding sequence (CDS)

ATGGACGAGATATTTCATGTACCTCAAGCTCAGAAATGCTGCAGTGGGAACTTCCGAAGTTGGGACCCGAAGATTTCAAATCCTCCTGGACTCTTTCACTTGCCCACGTTGCTTCTTTGTTTCCTGCCATTGTCTTTACTCAAGCCGTACCATCATTTTCTGAAGCTTGTTCCATTCATATTCTCCGTTCAACTAATTTGCATCATTTTCTAG

Protein sequence

MDEIFHVPQAQKCCSGNFRSWDPKISNPPGLFHLPTLLLCFLPLSLLKPYHHFLKLVPFIFSVQLICIIF
Homology
BLAST of Csor.00g200290 vs. ExPASy Swiss-Prot
Match: Q8L638 (Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Arabidopsis thaliana OX=3702 GN=ALG10 PE=1 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 9.6e-11
Identity = 26/34 (76.47%), Postives = 31/34 (91.18%), Query Frame = 0

Query: 1  MDEIFHVPQAQKCCSGNFRSWDPKISNPPGLFHL 35
          MDEIFHVPQAQ+ C+GNFRSWDP I+ PPGL++L
Sbjct: 30 MDEIFHVPQAQQYCNGNFRSWDPMITTPPGLYYL 63

BLAST of Csor.00g200290 vs. ExPASy Swiss-Prot
Match: Q4HZE0 (Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) OX=229533 GN=ALG10 PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 9.9e-08
Identity = 21/32 (65.62%), Postives = 25/32 (78.12%), Query Frame = 0

Query: 1   MDEIFHVPQAQKCCSGNFRSWDPKISNPPGLF 33
           +DEIFH+PQAQK C G F  WD KI+ PPGL+
Sbjct: 80  LDEIFHIPQAQKYCQGRFLEWDDKITTPPGLY 111

BLAST of Csor.00g200290 vs. ExPASy Swiss-Prot
Match: Q7SA35 (Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=alg-10 PE=3 SV=3)

HSP 1 Score: 53.9 bits (128), Expect = 8.4e-07
Identity = 19/34 (55.88%), Postives = 25/34 (73.53%), Query Frame = 0

Query: 1   MDEIFHVPQAQKCCSGNFRSWDPKISNPPGLFHL 35
           +DE+FH+PQAQ  C G +  WD KI+ PPGL+ L
Sbjct: 74  LDEVFHIPQAQTYCEGRYHEWDNKITTPPGLYLL 107

BLAST of Csor.00g200290 vs. ExPASy Swiss-Prot
Match: Q6CEA5 (Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=ALG10 PE=3 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 1.4e-06
Identity = 18/32 (56.25%), Postives = 26/32 (81.25%), Query Frame = 0

Query: 1  MDEIFHVPQAQKCCSGNFRSWDPKISNPPGLF 33
          +DEIFH+PQ Q+ C G++ +WD KI+ PPGL+
Sbjct: 40 IDEIFHIPQTQQYCKGHWNAWDSKITTPPGLY 71

BLAST of Csor.00g200290 vs. ExPASy Swiss-Prot
Match: P0C147 (Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) OX=242507 GN=ALG10 PE=3 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 2.1e-05
Identity = 18/39 (46.15%), Postives = 28/39 (71.79%), Query Frame = 0

Query: 1   MDEIFHVPQAQKCCSGNFRSWDPKISNPPGLFHLPTLLL 40
           +DE+FH+PQA+K   G +  WD KI+ PPGL+ +  +L+
Sbjct: 73  LDEVFHIPQAEKYLQGRWVEWDDKITTPPGLYLVSYVLV 111

BLAST of Csor.00g200290 vs. NCBI nr
Match: KAG6603654.1 (Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 149 bits (376), Expect = 2.32e-45
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 0

Query: 1  MDEIFHVPQAQKCCSGNFRSWDPKISNPPGLFHLPTLLLCFLPLSLLKPYHHFLKLVPFI 60
          MDEIFHVPQAQKCCSGNFRSWDPKISNPPGLFHLPTLLLCFLPLSLLKPYHHFLKLVPFI
Sbjct: 1  MDEIFHVPQAQKCCSGNFRSWDPKISNPPGLFHLPTLLLCFLPLSLLKPYHHFLKLVPFI 60

Query: 61 FSVQLICIIF 70
          FSVQLICIIF
Sbjct: 61 FSVQLICIIF 70

BLAST of Csor.00g200290 vs. NCBI nr
Match: KAG7033838.1 (hypothetical protein SDJN02_03563 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 76.6 bits (187), Expect = 2.92e-14
Identity = 32/37 (86.49%), Postives = 35/37 (94.59%), Query Frame = 0

Query: 1   MDEIFHVPQAQKCCSGNFRSWDPKISNPPGLFHLPTL 37
           MD+IFHVPQAQKCCSGNFRSWDPKISNPPGL+ L +L
Sbjct: 406 MDKIFHVPQAQKCCSGNFRSWDPKISNPPGLYVLISL 442

BLAST of Csor.00g200290 vs. NCBI nr
Match: XP_038877648.1 (dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase [Benincasa hispida])

HSP 1 Score: 69.3 bits (168), Expect = 1.16e-11
Identity = 28/34 (82.35%), Postives = 31/34 (91.18%), Query Frame = 0

Query: 1  MDEIFHVPQAQKCCSGNFRSWDPKISNPPGLFHL 34
          MDEIFHVPQAQK CSGNFRSWDP I+ PPGL++L
Sbjct: 30 MDEIFHVPQAQKYCSGNFRSWDPMITTPPGLYYL 63

BLAST of Csor.00g200290 vs. NCBI nr
Match: XP_022143861.1 (dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase isoform X1 [Momordica charantia])

HSP 1 Score: 68.9 bits (167), Expect = 1.58e-11
Identity = 27/34 (79.41%), Postives = 31/34 (91.18%), Query Frame = 0

Query: 1  MDEIFHVPQAQKCCSGNFRSWDPKISNPPGLFHL 34
          MDEIFH+PQAQK CSGNFRSWDP I+ PPGL++L
Sbjct: 30 MDEIFHIPQAQKYCSGNFRSWDPMITTPPGLYYL 63

BLAST of Csor.00g200290 vs. NCBI nr
Match: XP_022931874.1 (dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase [Cucurbita moschata])

HSP 1 Score: 68.9 bits (167), Expect = 1.58e-11
Identity = 27/34 (79.41%), Postives = 31/34 (91.18%), Query Frame = 0

Query: 1  MDEIFHVPQAQKCCSGNFRSWDPKISNPPGLFHL 34
          MDEIFH+PQAQK CSGNFRSWDP I+ PPGL++L
Sbjct: 30 MDEIFHIPQAQKYCSGNFRSWDPMITTPPGLYYL 63

BLAST of Csor.00g200290 vs. ExPASy TrEMBL
Match: A0A6J1CRK7 (Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Momordica charantia OX=3673 GN=LOC111013674 PE=3 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 7.66e-12
Identity = 27/34 (79.41%), Postives = 31/34 (91.18%), Query Frame = 0

Query: 1  MDEIFHVPQAQKCCSGNFRSWDPKISNPPGLFHL 34
          MDEIFH+PQAQK CSGNFRSWDP I+ PPGL++L
Sbjct: 30 MDEIFHIPQAQKYCSGNFRSWDPMITTPPGLYYL 63

BLAST of Csor.00g200290 vs. ExPASy TrEMBL
Match: A0A6J1HUC4 (Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111466254 PE=3 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 7.66e-12
Identity = 27/34 (79.41%), Postives = 31/34 (91.18%), Query Frame = 0

Query: 1  MDEIFHVPQAQKCCSGNFRSWDPKISNPPGLFHL 34
          MDEIFH+PQAQK CSGNFRSWDP I+ PPGL++L
Sbjct: 30 MDEIFHIPQAQKYCSGNFRSWDPMITTPPGLYYL 63

BLAST of Csor.00g200290 vs. ExPASy TrEMBL
Match: A0A6J1EVG0 (Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111438157 PE=3 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 7.66e-12
Identity = 27/34 (79.41%), Postives = 31/34 (91.18%), Query Frame = 0

Query: 1  MDEIFHVPQAQKCCSGNFRSWDPKISNPPGLFHL 34
          MDEIFH+PQAQK CSGNFRSWDP I+ PPGL++L
Sbjct: 30 MDEIFHIPQAQKYCSGNFRSWDPMITTPPGLYYL 63

BLAST of Csor.00g200290 vs. ExPASy TrEMBL
Match: A0A540MVQ2 (Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Malus baccata OX=106549 GN=C1H46_011558 PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.05e-11
Identity = 32/55 (58.18%), Postives = 41/55 (74.55%), Query Frame = 0

Query: 1  MDEIFHVPQAQKCCSGNFRSWDPKISNPPGLFHLPTLLLCFL--PLSLLKPYHHF 53
          MDEIFHVPQAQ+ C+GNFRSWDP I+ PPGL++L    + +L   +SL+K  H F
Sbjct: 30 MDEIFHVPQAQQYCNGNFRSWDPMITTPPGLYYLSLAHVAYLFPGMSLVKADHSF 84

BLAST of Csor.00g200290 vs. ExPASy TrEMBL
Match: A0A498K249 (Dolichyl-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-dolichol alpha-1,2-glucosyltransferase OS=Malus domestica OX=3750 GN=DVH24_026779 PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.08e-11
Identity = 32/55 (58.18%), Postives = 41/55 (74.55%), Query Frame = 0

Query: 1   MDEIFHVPQAQKCCSGNFRSWDPKISNPPGLFHLPTLLLCFL--PLSLLKPYHHF 53
           MDEIFHVPQAQ+ C+GNFRSWDP I+ PPGL++L    + +L   +SL+K  H F
Sbjct: 414 MDEIFHVPQAQQYCNGNFRSWDPMITTPPGLYYLSLAHVAYLFPGMSLVKADHSF 468

BLAST of Csor.00g200290 vs. TAIR 10
Match: AT5G02410.1 (DIE2/ALG10 family )

HSP 1 Score: 67.0 bits (162), Expect = 6.8e-12
Identity = 26/34 (76.47%), Postives = 31/34 (91.18%), Query Frame = 0

Query: 1  MDEIFHVPQAQKCCSGNFRSWDPKISNPPGLFHL 35
          MDEIFHVPQAQ+ C+GNFRSWDP I+ PPGL++L
Sbjct: 30 MDEIFHVPQAQQYCNGNFRSWDPMITTPPGLYYL 63

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L6389.6e-1176.47Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Arabidop... [more]
Q4HZE09.9e-0865.63Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Gibberel... [more]
Q7SA358.4e-0755.88Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Neurospo... [more]
Q6CEA51.4e-0656.25Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Yarrowia... [more]
P0C1472.1e-0546.15Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Magnapor... [more]
Match NameE-valueIdentityDescription
KAG6603654.12.32e-45100.00Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase, partial [C... [more]
KAG7033838.12.92e-1486.49hypothetical protein SDJN02_03563 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_038877648.11.16e-1182.35dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase [Benincasa ... [more]
XP_022143861.11.58e-1179.41dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase isoform X1 ... [more]
XP_022931874.11.58e-1179.41dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase [Cucurbita ... [more]
Match NameE-valueIdentityDescription
A0A6J1CRK77.66e-1279.41Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Momordic... [more]
A0A6J1HUC47.66e-1279.41Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Cucurbit... [more]
A0A6J1EVG07.66e-1279.41Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Cucurbit... [more]
A0A540MVQ21.05e-1158.18Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Malus ba... [more]
A0A498K2491.08e-1158.18Dolichyl-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-dolichol alpha-1,2-glucosyltransferase O... [more]
Match NameE-valueIdentityDescription
AT5G02410.16.8e-1276.47DIE2/ALG10 family [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016900Alpha-2-glucosyltransferase Alg10PFAMPF04922DIE2_ALG10coord: 1..66
e-value: 2.4E-15
score: 56.7

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g200290.m01Csor.00g200290.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006488 dolichol-linked oligosaccharide biosynthetic process
biological_process GO:0048366 leaf development
biological_process GO:0009651 response to salt stress
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0106073 dolichyl pyrophosphate Glc2Man9GlcNAc2 alpha-1,2-glucosyltransferase activity
molecular_function GO:0000166 nucleotide binding