Csor.00g192780 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g192780
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionGTP cyclohydrolase II
LocationCsor_Chr01: 3046077 .. 3049020 (+)
RNA-Seq ExpressionCsor.00g192780
SyntenyCsor.00g192780
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCTACTCTTCTTTCTCACCCTCTCCTTCCCCGTTTCACTAACTTGCCTCTTTCAAGGTACGCTTCTTTCCCTACTCACCCCTAATTTTGTGCTCATTTACGTGCCACTAAACTTGTTGTCTTTGTAGCTTTAATCGCCTCTCTTTGCCTCATCCTAAGATTGGCAAATGTAAGCAAAGGGGGCTGCGTTTTGTTTGCTCCGCTGCTGCTGACTTGTTTGATGACAACCTCGCCGCTAAAAATGGTAACGGTGCTGCTGCTGCTGCTGCTGCTGCCGCCGCTGCTTTAGGACTTGAGGCTGAAACCACGCCCGATGTTGTTGATTTCTTTGATGATGAGGACGATCCTGACTGCCCATCTGAGGGCTACTCTTCCATTCAGAACGCCGTTGATGCTATTAGCCAAGGAAAGGTTGCCAAATGTCCATTTCCTTATGGATAATACTTACTTACACGCTAATAAATTAATACTTTCTTTTTTCTACCTTATCCATTTCTCTTTGGATTTTGACAACCGTCTATTTCGTTTTGGAACGCTGAAGTCCACTTTTAGTAAATATTGTTTTTTTTTATGTCTTTTTTTCGGGTTTCTCCTCAAAGTTTTTTAAAAGCATAATGGTAGTGAGAGGTTTTCACATCTTTACGAATGATGTTTTGTTCTCCTCTCCAACCGATGTGAGATCTCACAATCCACCTCCTTCAAGGCACAACTTCGGTTCAGTGTCTGTCTCTGATATCATATGTAATAACCCAAACACACCACTAGCCGATATTAACCCTTTTGAATTTTTTCTTAAAATTTTTAAAACCTATCCATTAAGGAGAAGTTTCCACAGTCTCACAAAGGATGTTTCGCTTTCTTCTCGAACCTACGAGTGATCTTAAAACGTTACAAAAGAACAAAAATTATTTTCTCTTTGTACTTATTAGTTGAGGATGTGGCATGTTTGTATGGGTCTTTCTATTAATTTTCTTTTTCTTCTCTTACCCTTTTTGCATGCATTTCTCTTCTCTCTCCTCATGCATTTCTCTCACCTAATGGCCAAAATACTTCATTGTTTTCTAGTTTGTGATCGTTGTGGATGATGAAAATGAGGACACGGAAGGGAATTTGGTAATGGCTGCCTCTTTTACAACTCCTAGTAACATAACAACCATGGTTCAACATGGTTCAGGCATACTCTATGTGGGTATGAAAGAGGACGATCTCGAAAGGCTTAAGCTTCCCATAATATCGATCGATAATGGAAACGAGACATCAACTTTTACCATCACTGTGGTACGTAGTACACATGAGAAATTTGAACTTCTGACCTCTTAATTAAATTATTATACATGACCGCACCCATGTAGGGTTTATTACTCTGAGTGTTACTAATTTATTGTTTGGATGAGACAGGATTTGAAAAATGGGACATCAGCAGGAGTATCAACTGCAGATAGAGCTAAGACAATTGTTGCTTTATCATCTCCTGAGTCTAAGGCTGAGGACTTCAGACGGCCAGGTCACGTGTTTCCCCTCCGGTACCGAGACGGTGGCGTTCTGAGGAGAGCTGGCCACACGGAAGCTTCTGTAGATTTGGTTTCCCTTGCTGGGTTAAGGCCAGTTTCTGTTCTTTCAACCATTGTTGACCCTGAAGACGGCTCCATGGCTTCACTACCCAAGTTGAGAAAGTTGGCCTCAGAAAAGAATATCCCAATAGTCTCTATTACTGATTTGATTAGGTAAGTCATGTTGGTGGGTCGATAATTGATGTCTTATGCATTGAACTATAAAAAATTTGATTCACTTAATTTTTTTGGCTGTTTATTTTGGTAGATACCGAAGAAAGAGGGAAAATTTGATTGAAAGAATTGCTATTTCTCGGCTGCCAACTAAATGGGGCCAATTCTATGCTCACTGCTATCGCTCAAATCTAGATGGAATTGAGCACGTGGCTCTTGTAAAGGTAATGGAATTTGTTTTCTTTGAGGAATAATACATTCATTAACTCATGACATCCTTAAATATCACAAACATATGATAGTTGGTGGTTGGTGAGCTTCAAAACCCAATATTTAACCTAAAAAAATAATAAAATTAGCGTTAAATCTTCAGATCTAGTTTGATAGTCACTGGTCACTGTATTAGCATTGAGAATATTTAATTTTGAATTATAGTTTACCGTATATATCATATTTGACAATTTATTATAAGCAAATATCTTATATTTATTTACTGTTTTTTTTATTTCTTTAGTAGTTTATTTGATTCTATAAATGATTATAAATAGAAAACTTTCGCCCCTCTTTAGTTGTAGTAGTTTTAGTAAATATTCTTTCGATGCTCTTTTTTAATAGGGAAACATTGGGGACGGTCAAGATGTGCTTGTAAGAGTCCACTCCGAGTGCTTAACTGGCGACATATTCGCATCGGCAAGATGCGATTGTGGCAACCAACTAGATTTAGCAATGCAAATGATTGAGAAGGCTGGAAGAGGCGCTGTTATTTACCTAAGAGGTCATGAAGGAAGAGGCATTGGCCTTGGCCCCAAGCTGCTCGCTTACAACCTACAAGATCAAGGCCATGACACAGTCGAAGCCAACATCGAGCTAGGATTGCCTGCCGACGCCCGTGATTACGGAGTCGGAGCTCAGGTCAGTCAACTCAACTCGACTCGAGTTAACTAAAGTTAAATTTGATTTTGTTATTTTATTTGTGGATGATGTCAGATGCTGAGAGATATTGGGATTAGAACAATGCGGTTGATGACAAACAACCCCTCGAAGTTCAAGGGGCTGAAGGGATATGGTTTGGCAGTGACAGCGAGGGTCCCTGTGGTGACACCCATTACAGATGAAAACAGAAAGTATTTGGAAACCAAGAGAACCAAGATGGGCCATATCTATGCGAAGGGCCAATTTTTAAGGTCCCGACAGGCTGAAGCTTCAACAAATTGA

mRNA sequence

ATGGCTTCTACTCTTCTTTCTCACCCTCTCCTTCCCCGTTTCACTAACTTGCCTCTTTCAAGCTTTAATCGCCTCTCTTTGCCTCATCCTAAGATTGGCAAATGTAAGCAAAGGGGGCTGCGTTTTGTTTGCTCCGCTGCTGCTGACTTGTTTGATGACAACCTCGCCGCTAAAAATGGTAACGGTGCTGCTGCTGCTGCTGCTGCTGCCGCCGCTGCTTTAGGACTTGAGGCTGAAACCACGCCCGATGTTGTTGATTTCTTTGATGATGAGGACGATCCTGACTGCCCATCTGAGGGCTACTCTTCCATTCAGAACGCCGTTGATGCTATTAGCCAAGGAAAGTTTGTGATCGTTGTGGATGATGAAAATGAGGACACGGAAGGGAATTTGGTAATGGCTGCCTCTTTTACAACTCCTAGTAACATAACAACCATGGTTCAACATGGTTCAGGCATACTCTATGTGGGTATGAAAGAGGACGATCTCGAAAGGCTTAAGCTTCCCATAATATCGATCGATAATGGAAACGAGACATCAACTTTTACCATCACTGTGGATTTGAAAAATGGGACATCAGCAGGAGTATCAACTGCAGATAGAGCTAAGACAATTGTTGCTTTATCATCTCCTGAGTCTAAGGCTGAGGACTTCAGACGGCCAGGTCACGTGTTTCCCCTCCGGTACCGAGACGGTGGCGTTCTGAGGAGAGCTGGCCACACGGAAGCTTCTGTAGATTTGGTTTCCCTTGCTGGGTTAAGGCCAGTTTCTGTTCTTTCAACCATTGTTGACCCTGAAGACGGCTCCATGGCTTCACTACCCAAGTTGAGAAAGTTGGCCTCAGAAAAGAATATCCCAATAGTCTCTATTACTGATTTGATTAGATACCGAAGAAAGAGGGAAAATTTGATTGAAAGAATTGCTATTTCTCGGCTGCCAACTAAATGGGGCCAATTCTATGCTCACTGCTATCGCTCAAATCTAGATGGAATTGAGCACGTGGCTCTTGTAAAGGGAAACATTGGGGACGGTCAAGATGTGCTTGTAAGAGTCCACTCCGAGTGCTTAACTGGCGACATATTCGCATCGGCAAGATGCGATTGTGGCAACCAACTAGATTTAGCAATGCAAATGATTGAGAAGGCTGGAAGAGGCGCTGTTATTTACCTAAGAGGTCATGAAGGAAGAGGCATTGGCCTTGGCCCCAAGCTGCTCGCTTACAACCTACAAGATCAAGGCCATGACACAGTCGAAGCCAACATCGAGCTAGGATTGCCTGCCGACGCCCGTGATTACGGAGTCGGAGCTCAGATGCTGAGAGATATTGGGATTAGAACAATGCGGTTGATGACAAACAACCCCTCGAAGTTCAAGGGGCTGAAGGGATATGGTTTGGCAGTGACAGCGAGGGTCCCTGTGGTGACACCCATTACAGATGAAAACAGAAAGTATTTGGAAACCAAGAGAACCAAGATGGGCCATATCTATGCGAAGGGCCAATTTTTAAGGTCCCGACAGGCTGAAGCTTCAACAAATTGA

Coding sequence (CDS)

ATGGCTTCTACTCTTCTTTCTCACCCTCTCCTTCCCCGTTTCACTAACTTGCCTCTTTCAAGCTTTAATCGCCTCTCTTTGCCTCATCCTAAGATTGGCAAATGTAAGCAAAGGGGGCTGCGTTTTGTTTGCTCCGCTGCTGCTGACTTGTTTGATGACAACCTCGCCGCTAAAAATGGTAACGGTGCTGCTGCTGCTGCTGCTGCTGCCGCCGCTGCTTTAGGACTTGAGGCTGAAACCACGCCCGATGTTGTTGATTTCTTTGATGATGAGGACGATCCTGACTGCCCATCTGAGGGCTACTCTTCCATTCAGAACGCCGTTGATGCTATTAGCCAAGGAAAGTTTGTGATCGTTGTGGATGATGAAAATGAGGACACGGAAGGGAATTTGGTAATGGCTGCCTCTTTTACAACTCCTAGTAACATAACAACCATGGTTCAACATGGTTCAGGCATACTCTATGTGGGTATGAAAGAGGACGATCTCGAAAGGCTTAAGCTTCCCATAATATCGATCGATAATGGAAACGAGACATCAACTTTTACCATCACTGTGGATTTGAAAAATGGGACATCAGCAGGAGTATCAACTGCAGATAGAGCTAAGACAATTGTTGCTTTATCATCTCCTGAGTCTAAGGCTGAGGACTTCAGACGGCCAGGTCACGTGTTTCCCCTCCGGTACCGAGACGGTGGCGTTCTGAGGAGAGCTGGCCACACGGAAGCTTCTGTAGATTTGGTTTCCCTTGCTGGGTTAAGGCCAGTTTCTGTTCTTTCAACCATTGTTGACCCTGAAGACGGCTCCATGGCTTCACTACCCAAGTTGAGAAAGTTGGCCTCAGAAAAGAATATCCCAATAGTCTCTATTACTGATTTGATTAGATACCGAAGAAAGAGGGAAAATTTGATTGAAAGAATTGCTATTTCTCGGCTGCCAACTAAATGGGGCCAATTCTATGCTCACTGCTATCGCTCAAATCTAGATGGAATTGAGCACGTGGCTCTTGTAAAGGGAAACATTGGGGACGGTCAAGATGTGCTTGTAAGAGTCCACTCCGAGTGCTTAACTGGCGACATATTCGCATCGGCAAGATGCGATTGTGGCAACCAACTAGATTTAGCAATGCAAATGATTGAGAAGGCTGGAAGAGGCGCTGTTATTTACCTAAGAGGTCATGAAGGAAGAGGCATTGGCCTTGGCCCCAAGCTGCTCGCTTACAACCTACAAGATCAAGGCCATGACACAGTCGAAGCCAACATCGAGCTAGGATTGCCTGCCGACGCCCGTGATTACGGAGTCGGAGCTCAGATGCTGAGAGATATTGGGATTAGAACAATGCGGTTGATGACAAACAACCCCTCGAAGTTCAAGGGGCTGAAGGGATATGGTTTGGCAGTGACAGCGAGGGTCCCTGTGGTGACACCCATTACAGATGAAAACAGAAAGTATTTGGAAACCAAGAGAACCAAGATGGGCCATATCTATGCGAAGGGCCAATTTTTAAGGTCCCGACAGGCTGAAGCTTCAACAAATTGA

Protein sequence

MASTLLSHPLLPRFTNLPLSSFNRLSLPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNGNGAAAAAAAAAAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSIQNAVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIFASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPSKFKGLKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHIYAKGQFLRSRQAEASTN
Homology
BLAST of Csor.00g192780 vs. ExPASy Swiss-Prot
Match: Q9FN89 (Monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RIBA3 PE=1 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 1.8e-178
Identity = 311/453 (68.65%), Postives = 380/453 (83.89%), Query Frame = 0

Query: 49  DLFDDNLAAKNGNGAAAAAAAAAAALGLEAETTPDVVDFF--DDEDDPDCPSEGYSSIQN 108
           ++FD+N   K       +A+       ++AE TP+  DFF  D E DPDCP++GYSSI+ 
Sbjct: 51  NVFDENPVRKTDGSLFDSASFET----VDAEITPETDDFFVSDAEGDPDCPTQGYSSIEL 110

Query: 109 AVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERL 168
           A+ A+ +GKFVIVVDDE  D EGNL+MAA+ T+P +I  ++++GSGI+ VGMK+++LERL
Sbjct: 111 ALQALRKGKFVIVVDDETGDVEGNLIMAATLTSPKDIAFLIKNGSGIVSVGMKKENLERL 170

Query: 169 KLPIISIDNGNETS---TFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGH 228
            L ++S +  +E S   TFTITVD K+GTS GVS +DRA T++ALSS ++K +DFRRPGH
Sbjct: 171 SLTLMSPEMEDEDSSAPTFTITVDAKSGTSTGVSASDRAMTVLALSSLDAKPDDFRRPGH 230

Query: 229 VFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKLRKLASEK 288
           VFPL+YRDGGVLRRAGHTEASVDL+ LAGLRP+SVLS I+D EDGSMASLP ++KLA+E 
Sbjct: 231 VFPLKYRDGGVLRRAGHTEASVDLMILAGLRPLSVLSAILDQEDGSMASLPYMKKLATEH 290

Query: 289 NIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGD 348
           +IPIVS+TDLIRYRRKR+ L+ERI +SRLPTKWG F A+CYRS LDG E++ALVKGN+G+
Sbjct: 291 DIPIVSLTDLIRYRRKRDKLVERITVSRLPTKWGLFQAYCYRSKLDGTENIALVKGNVGN 350

Query: 349 GQDVLVRVHSECLTGDIFASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPK 408
           G+D+LVRVHSECLTGDIF SARCDCGNQLDLAM++IEK GRG V+YLRGHEGRGIGLG K
Sbjct: 351 GEDILVRVHSECLTGDIFGSARCDCGNQLDLAMELIEKEGRGVVVYLRGHEGRGIGLGHK 410

Query: 409 LLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPSKFKGLKGY 468
           L AYNLQD+GHDTV+AN+ELGL  D+R+YG+GAQMLRDIG+RTMRLMTNNP+KF GLKGY
Sbjct: 411 LRAYNLQDEGHDTVQANVELGLSIDSREYGIGAQMLRDIGVRTMRLMTNNPAKFTGLKGY 470

Query: 469 GLAVTARVPVVTPITDENRKYLETKRTKMGHIY 497
           GLAV  RVPVVTPIT ENR+Y+ETKR KMGHIY
Sbjct: 471 GLAVVGRVPVVTPITKENRRYMETKRKKMGHIY 499

BLAST of Csor.00g192780 vs. ExPASy Swiss-Prot
Match: Q6L506 (Probable monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RIBA3 PE=2 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 4.1e-167
Identity = 294/454 (64.76%), Postives = 368/454 (81.06%), Query Frame = 0

Query: 49  DLFDDNLAAKNGNGAAAAAAAAAAALGLEAETTPDVVDFF--DDEDDPDCPSEGYSSIQN 108
           +L D    + NG        ++A+   + A+ T +  DFF  D E DPD P++G+SSI  
Sbjct: 55  NLNDPLKESNNGPVLQGFNGSSASFRTVGAKITQETGDFFVSDAEGDPDKPTDGFSSIDE 114

Query: 109 AVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERL 168
           A+ A+ +GKFVI VDDE+ D EG+LVMAA+   P +I  M+++GSGI+ VGMKE+DL RL
Sbjct: 115 AIGALHEGKFVIAVDDESGDNEGDLVMAATLADPESIAFMIRNGSGIISVGMKEEDLTRL 174

Query: 169 KLPIIS----IDNGNETSTFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPG 228
            +P++S    I++ +  ++ T+TVD + G S GVS ADRAKTI  L+SP+SK  D RRPG
Sbjct: 175 MIPMMSPIAEIEDISAAAS-TVTVDARVGISTGVSAADRAKTIFTLASPDSKPTDLRRPG 234

Query: 229 HVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKLRKLASE 288
           H+FPL+YR+GGVL+RAGHTEASVDLV+LAGLRPVSVLST+++P DGSMA +P L+++A E
Sbjct: 235 HIFPLKYRNGGVLKRAGHTEASVDLVALAGLRPVSVLSTVINPVDGSMAGMPVLKQMALE 294

Query: 289 KNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIG 348
            +IPIVSI DLIRYRRKRE L+E IA+SRLPTKWG F A+CY+S LDG EH+A+ KG+IG
Sbjct: 295 HDIPIVSIADLIRYRRKREKLVELIAVSRLPTKWGLFRAYCYQSKLDGTEHIAVAKGDIG 354

Query: 349 DGQDVLVRVHSECLTGDIFASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGP 408
           DG+DVLVRVHSECLTGDI  SARCDCGNQLDLAMQ+I+KAGRG ++YLRGHEGRGIGLG 
Sbjct: 355 DGEDVLVRVHSECLTGDILGSARCDCGNQLDLAMQLIDKAGRGVLVYLRGHEGRGIGLGQ 414

Query: 409 KLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPSKFKGLKG 468
           KL AYNLQD GHDTV+AN+ELGL  D+R+YG+GAQ+LRD+G+RTMRLMTNNP+KF GLKG
Sbjct: 415 KLRAYNLQDDGHDTVQANVELGLAVDSREYGIGAQILRDMGVRTMRLMTNNPAKFVGLKG 474

Query: 469 YGLAVTARVPVVTPITDENRKYLETKRTKMGHIY 497
           YGLAV  RVPV++PIT EN++YLETKRTKMGH+Y
Sbjct: 475 YGLAVVGRVPVISPITKENQRYLETKRTKMGHVY 507

BLAST of Csor.00g192780 vs. ExPASy Swiss-Prot
Match: Q6Z234 (Probable bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RIBA1 PE=2 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 9.4e-164
Identity = 292/493 (59.23%), Postives = 369/493 (74.85%), Query Frame = 0

Query: 10  LLPRFTNLPLSSFNRLSLPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNGNGAAAAAAA 69
           ++P  T+  L + + + LP+        R   F  S A     +++        +    A
Sbjct: 22  IMPNSTSNSLRTISSVHLPY------NHRARNFHISHAVGDSSEHVIINGQASPSKVVQA 81

Query: 70  AAAALGLEAETTPDVVDFF---DDEDDPDCPSEGYSSIQNAVDAISQGKFVIVVDDENED 129
            AAALG  A     VVD F   DDE D D P+EG+SSI  A++ I QGK+VIVVDDE+ +
Sbjct: 82  DAAALGTIAADMAPVVDGFSADDDELDLDSPTEGFSSIPEAIEDIRQGKYVIVVDDEDRE 141

Query: 130 TEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNE---TSTFT 189
            EG+L+MAAS  TP  +  +V+HG+GI+ V MKEDDLERL+LP++     NE    + FT
Sbjct: 142 NEGDLIMAASKVTPEAMAFIVRHGTGIVCVSMKEDDLERLELPLMVTTKENEEKLRTAFT 201

Query: 190 ITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHTEA 249
           ++VD K GT+ GVS  DRA T++AL+SP SK EDF RPGH+FPL+YR+GGVL+RAGHTEA
Sbjct: 202 VSVDAKEGTTTGVSAKDRANTVLALASPNSKPEDFNRPGHIFPLKYREGGVLKRAGHTEA 261

Query: 250 SVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKRENL 309
           SVDL  LAGL P +VL  IVD +DGSMA LPKL+  A  +N+ I+SI DLIRYRRKR+ L
Sbjct: 262 SVDLAMLAGLPPAAVLCEIVDDDDGSMALLPKLQDFARRENLKIISIADLIRYRRKRDRL 321

Query: 310 IERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIFAS 369
           +ER+ ++ L  +WG F ++CYRS +DG+EH+A+VKG++GDGQD+LVRVHSECLTGDIF S
Sbjct: 322 VERVCVTPLQLQWGSFQSYCYRSLIDGMEHIAMVKGDVGDGQDILVRVHSECLTGDIFGS 381

Query: 370 ARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIEL 429
           ARCDCGNQL LAM MIEK GRG V+YLRGHEGRGIGLG KL AYNLQD G DTVEAN +L
Sbjct: 382 ARCDCGNQLALAMTMIEKTGRGVVVYLRGHEGRGIGLGHKLRAYNLQDDGRDTVEANEDL 441

Query: 430 GLPADARDYGVGAQMLRDIGIRTMRLMTNNPSKFKGLKGYGLAVTARVPVVTPITDENRK 489
           GLP D+R+YG+GAQ+LRD+G+RTMRLMTNNP+K+ GLKGYGL+V  RVP++TPIT+ENR+
Sbjct: 442 GLPVDSREYGIGAQILRDLGVRTMRLMTNNPAKYTGLKGYGLSVLGRVPLLTPITNENRR 501

Query: 490 YLETKRTKMGHIY 497
           Y+ETKR KMGH+Y
Sbjct: 502 YMETKRLKMGHVY 508

BLAST of Csor.00g192780 vs. ExPASy Swiss-Prot
Match: Q0E079 (Probable bifunctional riboflavin biosynthesis protein RIBA 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RIBA2 PE=2 SV=1)

HSP 1 Score: 572.4 bits (1474), Expect = 5.1e-162
Identity = 282/427 (66.04%), Postives = 343/427 (80.33%), Query Frame = 0

Query: 84  VVDFFDDED-----DPDCPSEGYSSIQNAVDAISQGKFVIVVDDENEDTEGNLVMAASFT 143
           V D F DED     D D P+EG+SSI  A+  I QGK VIVVDDE+ + EG+L+MAAS  
Sbjct: 114 VADMFSDEDKDTEQDLDSPTEGFSSISEAIKDIQQGKLVIVVDDESRENEGDLIMAASLV 173

Query: 144 TPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNE---TSTFTITVDLKNGTSAG 203
           TP  +  +V++G+GI+ V MKE+DLERL LP++     NE    + FT+TVD K GT+ G
Sbjct: 174 TPEAMAFVVRYGTGIVCVSMKEEDLERLNLPLMVATKENEEKLCTAFTVTVDAKEGTTTG 233

Query: 204 VSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRP 263
           VS  DRAKT++ L+SP+SK EDF RPGH+FPL+YR+GGVL+RAGHTEASVDL  LAGL P
Sbjct: 234 VSAKDRAKTVMTLASPDSKPEDFNRPGHIFPLKYREGGVLKRAGHTEASVDLAMLAGLPP 293

Query: 264 VSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTK 323
            +VL  IVD EDGSMA LPKLR  A  +N+ IVSI DLIRYRRKR+ L+ER +++RLP +
Sbjct: 294 AAVLCEIVD-EDGSMARLPKLRVFAERENLKIVSIADLIRYRRKRDRLVERSSVARLPLR 353

Query: 324 WGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIFASARCDCGNQLDLA 383
           WG   A+CYRS +DGIEH+A+VKG IGDGQ VLVRVHSECLTGDIF SARCDCG+QL +A
Sbjct: 354 WGNVRAYCYRSVIDGIEHIAMVKGEIGDGQGVLVRVHSECLTGDIFGSARCDCGDQLAMA 413

Query: 384 MQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVG 443
           M+MIEKAGRG ++YLRGHEGRGIGLG KL AYNLQD G DTVEAN +LGLP D+R+YG+G
Sbjct: 414 MEMIEKAGRGVLVYLRGHEGRGIGLGHKLRAYNLQDDGRDTVEANEDLGLPVDSREYGIG 473

Query: 444 AQMLRDIGIRTMRLMTNNPSKFKGLKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHI 501
           AQ+LRD+G+R+M+LMTNNP+K+ GLKGYGL++  RVP+VTPIT ENR+YLETKRTKMGH+
Sbjct: 474 AQILRDLGVRSMKLMTNNPAKYGGLKGYGLSIVGRVPLVTPITSENRRYLETKRTKMGHV 533

BLAST of Csor.00g192780 vs. ExPASy Swiss-Prot
Match: P47924 (Bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RIBA1 PE=1 SV=2)

HSP 1 Score: 560.8 bits (1444), Expect = 1.5e-158
Identity = 305/535 (57.01%), Postives = 386/535 (72.15%), Query Frame = 0

Query: 2   ASTLLSHPL----LPRFTNLPLSSFNRLSLPHPKIGKCKQRGLRFVCSAAADLFDDNLAA 61
           +ST L H L    LP  +N PLS+F+  +      GK K        +A     DD L+ 
Sbjct: 24  SSTTLLHGLHRVTLP--SNHPLSTFSIKT----NTGKVK--------AAVISREDDLLSF 83

Query: 62  KNGNGAAAAAA-----------AAAAALG-LEAETTPDVVDFFDDEDDP---DCPSEGYS 121
            NGN   +  +           A + +LG L A++ P   + F  EDD    D P+ G+S
Sbjct: 84  TNGNTPLSNGSLIDDRTEEPLEADSVSLGTLAADSAPAPANGFVAEDDDFELDLPTPGFS 143

Query: 122 SIQNAVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDD 181
           SI  A++ I QGK V+VVDDE+ + EG+LVMAA   TP  +  +V+HG+GI+ V MKEDD
Sbjct: 144 SIPEAIEDIRQGKLVVVVDDEDRENEGDLVMAAQLATPEAMAFIVRHGTGIVCVSMKEDD 203

Query: 182 LERLKLPIISIDNGNE---TSTFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFR 241
           LERL LP++     NE   ++ FT+TVD K+GT+ GVS  DRA TI++L+S +SK EDF 
Sbjct: 204 LERLHLPLMVNQKENEEKLSTAFTVTVDAKHGTTTGVSARDRATTILSLASRDSKPEDFN 263

Query: 242 RPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKLRKL 301
           RPGH+FPL+YR+GGVL+RAGHTEASVDL  LAGL PV VL  IVD +DGSMA LPKLR+ 
Sbjct: 264 RPGHIFPLKYREGGVLKRAGHTEASVDLTVLAGLDPVGVLCEIVD-DDGSMARLPKLREF 323

Query: 302 ASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKG 361
           A+E N+ +VSI DLIRYRRKR+ L+ER + +R+PT WG F A+CYRS LDGIEH+A+VKG
Sbjct: 324 AAENNLKVVSIADLIRYRRKRDKLVERASAARIPTMWGPFTAYCYRSILDGIEHIAMVKG 383

Query: 362 NIGDGQDVLVRVHSECLTGDIFASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIG 421
            IGDGQD+LVRVHSECLTGDIF SARCDCGNQL L+MQ IE  GRG ++YLRGHEGRGIG
Sbjct: 384 EIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALSMQQIEATGRGVLVYLRGHEGRGIG 443

Query: 422 LGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPSKFKG 481
           LG KL AYNLQD G DTVEAN ELGLP D+R+YG+GAQ++RD+G+RTM+LMTNNP+K+ G
Sbjct: 444 LGHKLRAYNLQDAGRDTVEANEELGLPVDSREYGIGAQIIRDLGVRTMKLMTNNPAKYVG 503

Query: 482 LKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHIYA---KGQFLRSRQAEAST 512
           LKGYGLA+  RVP+++ IT EN++YLETKRTKMGH+Y    KG  +   ++E+ +
Sbjct: 504 LKGYGLAIVGRVPLLSLITKENKRYLETKRTKMGHMYGLKFKGDVVEKIESESES 543

BLAST of Csor.00g192780 vs. NCBI nr
Match: KAG6607228.1 (Monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 998 bits (2579), Expect = 0.0
Identity = 512/512 (100.00%), Postives = 512/512 (100.00%), Query Frame = 0

Query: 1   MASTLLSHPLLPRFTNLPLSSFNRLSLPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNG 60
           MASTLLSHPLLPRFTNLPLSSFNRLSLPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNG
Sbjct: 1   MASTLLSHPLLPRFTNLPLSSFNRLSLPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNG 60

Query: 61  NGAAAAAAAAAAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSIQNAVDAISQGKFVIVV 120
           NGAAAAAAAAAAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSIQNAVDAISQGKFVIVV
Sbjct: 61  NGAAAAAAAAAAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSIQNAVDAISQGKFVIVV 120

Query: 121 DDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETS 180
           DDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETS
Sbjct: 121 DDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETS 180

Query: 181 TFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGH 240
           TFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGH
Sbjct: 181 TFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGH 240

Query: 241 TEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKR 300
           TEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKR
Sbjct: 241 TEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKR 300

Query: 301 ENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDI 360
           ENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDI
Sbjct: 301 ENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDI 360

Query: 361 FASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEAN 420
           FASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEAN
Sbjct: 361 FASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEAN 420

Query: 421 IELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPSKFKGLKGYGLAVTARVPVVTPITDE 480
           IELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPSKFKGLKGYGLAVTARVPVVTPITDE
Sbjct: 421 IELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPSKFKGLKGYGLAVTARVPVVTPITDE 480

Query: 481 NRKYLETKRTKMGHIYAKGQFLRSRQAEASTN 512
           NRKYLETKRTKMGHIYAKGQFLRSRQAEASTN
Sbjct: 481 NRKYLETKRTKMGHIYAKGQFLRSRQAEASTN 512

BLAST of Csor.00g192780 vs. NCBI nr
Match: XP_022948312.1 (monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 984 bits (2544), Expect = 0.0
Identity = 507/512 (99.02%), Postives = 509/512 (99.41%), Query Frame = 0

Query: 1   MASTLLSHPLLPRFTNLPLSSFNRLSLPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNG 60
           MASTLLSHPLLPRFTNLPLSSFNR +LPHPKIGKCK RGLRFVCSAAADLFDDNLAAKNG
Sbjct: 1   MASTLLSHPLLPRFTNLPLSSFNRPALPHPKIGKCKLRGLRFVCSAAADLFDDNLAAKNG 60

Query: 61  NGAAAAAAAAAAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSIQNAVDAISQGKFVIVV 120
           NGAAAAAAAAAA LGLEAETTPDVVDFFDDEDDPDCPSEGYSSIQNAVDAISQGKFVIVV
Sbjct: 61  NGAAAAAAAAAA-LGLEAETTPDVVDFFDDEDDPDCPSEGYSSIQNAVDAISQGKFVIVV 120

Query: 121 DDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETS 180
           DDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETS
Sbjct: 121 DDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETS 180

Query: 181 TFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGH 240
           TFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGH
Sbjct: 181 TFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGH 240

Query: 241 TEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKR 300
           TEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKR
Sbjct: 241 TEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKR 300

Query: 301 ENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDI 360
           ENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDI
Sbjct: 301 ENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDI 360

Query: 361 FASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEAN 420
           FASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEAN
Sbjct: 361 FASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEAN 420

Query: 421 IELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPSKFKGLKGYGLAVTARVPVVTPITDE 480
           IELGLPADARDYGVGAQMLRDIGIRTMRLMTNNP+KFKGLKGYGLAVTARVPVVTPITDE
Sbjct: 421 IELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDE 480

Query: 481 NRKYLETKRTKMGHIYAKGQFLRSRQAEASTN 512
           NRKYLETKRTKMGHIYAKGQFLRSRQAEASTN
Sbjct: 481 NRKYLETKRTKMGHIYAKGQFLRSRQAEASTN 511

BLAST of Csor.00g192780 vs. NCBI nr
Match: XP_023522983.1 (monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 976 bits (2524), Expect = 0.0
Identity = 502/512 (98.05%), Postives = 506/512 (98.83%), Query Frame = 0

Query: 1   MASTLLSHPLLPRFTNLPLSSFNRLSLPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNG 60
           MAS LLSHPLLPRFTNLPLSSFNR +LPHPKIGKCK RGLRFVCSAAADLFDDNLAAKNG
Sbjct: 1   MASALLSHPLLPRFTNLPLSSFNRPALPHPKIGKCKLRGLRFVCSAAADLFDDNLAAKNG 60

Query: 61  NGAAAAAAAAAAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSIQNAVDAISQGKFVIVV 120
           NGAAAAAAAAA  LGLEAETTPDVVDFFDDEDDPDCPS+GYSSIQ AVDAISQGKFVIVV
Sbjct: 61  NGAAAAAAAAA--LGLEAETTPDVVDFFDDEDDPDCPSDGYSSIQTAVDAISQGKFVIVV 120

Query: 121 DDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETS 180
           DDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETS
Sbjct: 121 DDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETS 180

Query: 181 TFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGH 240
           TFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGH
Sbjct: 181 TFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGH 240

Query: 241 TEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKR 300
           TEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPK+RKLASEKNIPIVSITDLIRYRRKR
Sbjct: 241 TEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKMRKLASEKNIPIVSITDLIRYRRKR 300

Query: 301 ENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDI 360
           ENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDI
Sbjct: 301 ENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDI 360

Query: 361 FASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEAN 420
           FASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEAN
Sbjct: 361 FASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEAN 420

Query: 421 IELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPSKFKGLKGYGLAVTARVPVVTPITDE 480
           IELGLPADARDYGVGAQMLRDIGIRTMRLMTNNP+KFKGLKGYGLAVTARVPVVTPITDE
Sbjct: 421 IELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDE 480

Query: 481 NRKYLETKRTKMGHIYAKGQFLRSRQAEASTN 512
           NRKYLETKRTKMGHIYAKGQFLRSRQAEASTN
Sbjct: 481 NRKYLETKRTKMGHIYAKGQFLRSRQAEASTN 510

BLAST of Csor.00g192780 vs. NCBI nr
Match: XP_022998776.1 (monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 963 bits (2490), Expect = 0.0
Identity = 495/512 (96.68%), Postives = 501/512 (97.85%), Query Frame = 0

Query: 1   MASTLLSHPLLPRFTNLPLSSFNRLSLPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNG 60
           MAS LL+HPLLPRFTNLPLSSF+R +LPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNG
Sbjct: 1   MASALLAHPLLPRFTNLPLSSFHRPALPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNG 60

Query: 61  NGAAAAAAAAAAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSIQNAVDAISQGKFVIVV 120
           NGAA+AAA     LGLEAETTPDVVDFFDDEDDPDCPSEGYSSI  AVDAISQGKFVIVV
Sbjct: 61  NGAASAAA-----LGLEAETTPDVVDFFDDEDDPDCPSEGYSSIPTAVDAISQGKFVIVV 120

Query: 121 DDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETS 180
           DDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETS
Sbjct: 121 DDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETS 180

Query: 181 TFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGH 240
           TFTITVDLKNGTSAGVSTADRAKTIVALSSP+SKAEDFRRPGHVFPLRYRDGGVLRRAGH
Sbjct: 181 TFTITVDLKNGTSAGVSTADRAKTIVALSSPQSKAEDFRRPGHVFPLRYRDGGVLRRAGH 240

Query: 241 TEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKR 300
           TEASVDLVSLAGLR VSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKR
Sbjct: 241 TEASVDLVSLAGLRSVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKR 300

Query: 301 ENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDI 360
           ENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDI
Sbjct: 301 ENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDI 360

Query: 361 FASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEAN 420
           FASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEAN
Sbjct: 361 FASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEAN 420

Query: 421 IELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPSKFKGLKGYGLAVTARVPVVTPITDE 480
           IELGLPADARDYGVGAQMLRDIGIRTMRLMTNNP+KFKGLKGYGLAVTARVPVVTPITDE
Sbjct: 421 IELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDE 480

Query: 481 NRKYLETKRTKMGHIYAKGQFLRSRQAEASTN 512
           NRKYLETKRTKMGHIYAKGQFLRSRQAEAS N
Sbjct: 481 NRKYLETKRTKMGHIYAKGQFLRSRQAEASRN 507

BLAST of Csor.00g192780 vs. NCBI nr
Match: XP_022159223.1 (monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic-like [Momordica charantia])

HSP 1 Score: 785 bits (2028), Expect = 2.01e-282
Identity = 412/508 (81.10%), Postives = 444/508 (87.40%), Query Frame = 0

Query: 1   MASTLLSHPLLPRFTNLPLSSFNR---LSLPHPK-IGKCKQRGLRFVCSAAA----DLFD 60
           M S LL HPL  RF NL LS+FN     + P P+ + K KQR      SAAA    ++FD
Sbjct: 1   MDSALLCHPLFTRFNNLTLSTFNNRPSFAPPPPQTLRKSKQRTSNSASSAAAFSAGNMFD 60

Query: 61  DNLAAKNGNGAAAAAAAAAAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSIQNAVDAIS 120
           +    KNG       + AA+ LGL AET+ + +DFFD+ +DPD PS+GYSSIQ+AVDAI 
Sbjct: 61  ETPTTKNGK-----PSVAASTLGLGAETSSEAIDFFDNLEDPDHPSDGYSSIQSAVDAIH 120

Query: 121 QGKFVIVVDDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIIS 180
           QGKFVIVVDDEN D+EGNLVMAASF TP N+T+MVQHGSGI+YVGMKEDDL+RLKLP+ S
Sbjct: 121 QGKFVIVVDDENGDSEGNLVMAASFATPGNVTSMVQHGSGIVYVGMKEDDLQRLKLPLFS 180

Query: 181 IDNGN-ETSTFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRD 240
           +DNGN ETSTFTITVD+K GTS GVS ADRAKTIVALSS ESK EDFRRPGHVFP+RYRD
Sbjct: 181 VDNGNNETSTFTITVDVKTGTSGGVSAADRAKTIVALSSSESKPEDFRRPGHVFPVRYRD 240

Query: 241 GGVLRRAGHTEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSIT 300
           GGVLRRAGHTEASVDLV+LAGLRPVSVLS IVD +DGSMASLPKLR LAS+  IP+VSIT
Sbjct: 241 GGVLRRAGHTEASVDLVALAGLRPVSVLSAIVDTQDGSMASLPKLRSLASQHQIPMVSIT 300

Query: 301 DLIRYRRKRENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRV 360
           DLIRYRRKRENLIERIAISRLPTKWGQFYAHCYRS +DGIEHVALVKGNIGDGQDVLVRV
Sbjct: 301 DLIRYRRKRENLIERIAISRLPTKWGQFYAHCYRSKVDGIEHVALVKGNIGDGQDVLVRV 360

Query: 361 HSECLTGDIFASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQD 420
           HSECLTGDIFASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQD
Sbjct: 361 HSECLTGDIFASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQD 420

Query: 421 QGHDTVEANIELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPSKFKGLKGYGLAVTARV 480
           QGHDTVEAN+ELGLPADARDYGVGAQMLRDIGIRTMRLMTNNP+KFKGLKGYGLAVT RV
Sbjct: 421 QGHDTVEANLELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTGRV 480

Query: 481 PVVTPITDENRKYLETKRTKMGHIYAKG 499
           PVVT ITDENRKYLETKRTKMGHIYAKG
Sbjct: 481 PVVTQITDENRKYLETKRTKMGHIYAKG 503

BLAST of Csor.00g192780 vs. ExPASy TrEMBL
Match: A0A6J1G8U4 (GTP cyclohydrolase II OS=Cucurbita moschata OX=3662 GN=LOC111452026 PE=3 SV=1)

HSP 1 Score: 984 bits (2544), Expect = 0.0
Identity = 507/512 (99.02%), Postives = 509/512 (99.41%), Query Frame = 0

Query: 1   MASTLLSHPLLPRFTNLPLSSFNRLSLPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNG 60
           MASTLLSHPLLPRFTNLPLSSFNR +LPHPKIGKCK RGLRFVCSAAADLFDDNLAAKNG
Sbjct: 1   MASTLLSHPLLPRFTNLPLSSFNRPALPHPKIGKCKLRGLRFVCSAAADLFDDNLAAKNG 60

Query: 61  NGAAAAAAAAAAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSIQNAVDAISQGKFVIVV 120
           NGAAAAAAAAAA LGLEAETTPDVVDFFDDEDDPDCPSEGYSSIQNAVDAISQGKFVIVV
Sbjct: 61  NGAAAAAAAAAA-LGLEAETTPDVVDFFDDEDDPDCPSEGYSSIQNAVDAISQGKFVIVV 120

Query: 121 DDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETS 180
           DDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETS
Sbjct: 121 DDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETS 180

Query: 181 TFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGH 240
           TFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGH
Sbjct: 181 TFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGH 240

Query: 241 TEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKR 300
           TEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKR
Sbjct: 241 TEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKR 300

Query: 301 ENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDI 360
           ENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDI
Sbjct: 301 ENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDI 360

Query: 361 FASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEAN 420
           FASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEAN
Sbjct: 361 FASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEAN 420

Query: 421 IELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPSKFKGLKGYGLAVTARVPVVTPITDE 480
           IELGLPADARDYGVGAQMLRDIGIRTMRLMTNNP+KFKGLKGYGLAVTARVPVVTPITDE
Sbjct: 421 IELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDE 480

Query: 481 NRKYLETKRTKMGHIYAKGQFLRSRQAEASTN 512
           NRKYLETKRTKMGHIYAKGQFLRSRQAEASTN
Sbjct: 481 NRKYLETKRTKMGHIYAKGQFLRSRQAEASTN 511

BLAST of Csor.00g192780 vs. ExPASy TrEMBL
Match: A0A6J1KB55 (GTP cyclohydrolase II OS=Cucurbita maxima OX=3661 GN=LOC111493343 PE=3 SV=1)

HSP 1 Score: 963 bits (2490), Expect = 0.0
Identity = 495/512 (96.68%), Postives = 501/512 (97.85%), Query Frame = 0

Query: 1   MASTLLSHPLLPRFTNLPLSSFNRLSLPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNG 60
           MAS LL+HPLLPRFTNLPLSSF+R +LPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNG
Sbjct: 1   MASALLAHPLLPRFTNLPLSSFHRPALPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNG 60

Query: 61  NGAAAAAAAAAAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSIQNAVDAISQGKFVIVV 120
           NGAA+AAA     LGLEAETTPDVVDFFDDEDDPDCPSEGYSSI  AVDAISQGKFVIVV
Sbjct: 61  NGAASAAA-----LGLEAETTPDVVDFFDDEDDPDCPSEGYSSIPTAVDAISQGKFVIVV 120

Query: 121 DDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETS 180
           DDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETS
Sbjct: 121 DDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETS 180

Query: 181 TFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGH 240
           TFTITVDLKNGTSAGVSTADRAKTIVALSSP+SKAEDFRRPGHVFPLRYRDGGVLRRAGH
Sbjct: 181 TFTITVDLKNGTSAGVSTADRAKTIVALSSPQSKAEDFRRPGHVFPLRYRDGGVLRRAGH 240

Query: 241 TEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKR 300
           TEASVDLVSLAGLR VSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKR
Sbjct: 241 TEASVDLVSLAGLRSVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKR 300

Query: 301 ENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDI 360
           ENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDI
Sbjct: 301 ENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDI 360

Query: 361 FASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEAN 420
           FASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEAN
Sbjct: 361 FASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEAN 420

Query: 421 IELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPSKFKGLKGYGLAVTARVPVVTPITDE 480
           IELGLPADARDYGVGAQMLRDIGIRTMRLMTNNP+KFKGLKGYGLAVTARVPVVTPITDE
Sbjct: 421 IELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDE 480

Query: 481 NRKYLETKRTKMGHIYAKGQFLRSRQAEASTN 512
           NRKYLETKRTKMGHIYAKGQFLRSRQAEAS N
Sbjct: 481 NRKYLETKRTKMGHIYAKGQFLRSRQAEASRN 507

BLAST of Csor.00g192780 vs. ExPASy TrEMBL
Match: A0A6J1DY85 (GTP cyclohydrolase II OS=Momordica charantia OX=3673 GN=LOC111025642 PE=3 SV=1)

HSP 1 Score: 785 bits (2028), Expect = 9.72e-283
Identity = 412/508 (81.10%), Postives = 444/508 (87.40%), Query Frame = 0

Query: 1   MASTLLSHPLLPRFTNLPLSSFNR---LSLPHPK-IGKCKQRGLRFVCSAAA----DLFD 60
           M S LL HPL  RF NL LS+FN     + P P+ + K KQR      SAAA    ++FD
Sbjct: 1   MDSALLCHPLFTRFNNLTLSTFNNRPSFAPPPPQTLRKSKQRTSNSASSAAAFSAGNMFD 60

Query: 61  DNLAAKNGNGAAAAAAAAAAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSIQNAVDAIS 120
           +    KNG       + AA+ LGL AET+ + +DFFD+ +DPD PS+GYSSIQ+AVDAI 
Sbjct: 61  ETPTTKNGK-----PSVAASTLGLGAETSSEAIDFFDNLEDPDHPSDGYSSIQSAVDAIH 120

Query: 121 QGKFVIVVDDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIIS 180
           QGKFVIVVDDEN D+EGNLVMAASF TP N+T+MVQHGSGI+YVGMKEDDL+RLKLP+ S
Sbjct: 121 QGKFVIVVDDENGDSEGNLVMAASFATPGNVTSMVQHGSGIVYVGMKEDDLQRLKLPLFS 180

Query: 181 IDNGN-ETSTFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRD 240
           +DNGN ETSTFTITVD+K GTS GVS ADRAKTIVALSS ESK EDFRRPGHVFP+RYRD
Sbjct: 181 VDNGNNETSTFTITVDVKTGTSGGVSAADRAKTIVALSSSESKPEDFRRPGHVFPVRYRD 240

Query: 241 GGVLRRAGHTEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSIT 300
           GGVLRRAGHTEASVDLV+LAGLRPVSVLS IVD +DGSMASLPKLR LAS+  IP+VSIT
Sbjct: 241 GGVLRRAGHTEASVDLVALAGLRPVSVLSAIVDTQDGSMASLPKLRSLASQHQIPMVSIT 300

Query: 301 DLIRYRRKRENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRV 360
           DLIRYRRKRENLIERIAISRLPTKWGQFYAHCYRS +DGIEHVALVKGNIGDGQDVLVRV
Sbjct: 301 DLIRYRRKRENLIERIAISRLPTKWGQFYAHCYRSKVDGIEHVALVKGNIGDGQDVLVRV 360

Query: 361 HSECLTGDIFASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQD 420
           HSECLTGDIFASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQD
Sbjct: 361 HSECLTGDIFASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQD 420

Query: 421 QGHDTVEANIELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPSKFKGLKGYGLAVTARV 480
           QGHDTVEAN+ELGLPADARDYGVGAQMLRDIGIRTMRLMTNNP+KFKGLKGYGLAVT RV
Sbjct: 421 QGHDTVEANLELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTGRV 480

Query: 481 PVVTPITDENRKYLETKRTKMGHIYAKG 499
           PVVT ITDENRKYLETKRTKMGHIYAKG
Sbjct: 481 PVVTQITDENRKYLETKRTKMGHIYAKG 503

BLAST of Csor.00g192780 vs. ExPASy TrEMBL
Match: A0A6J1DXS1 (GTP cyclohydrolase II OS=Momordica charantia OX=3673 GN=LOC111024475 PE=3 SV=1)

HSP 1 Score: 721 bits (1861), Expect = 3.56e-257
Identity = 387/519 (74.57%), Postives = 431/519 (83.04%), Query Frame = 0

Query: 1   MASTLLSHP-LLPRFTNLPLS--SFNRLSLPHP-------KIGKCKQRGLRFVCSAAA-- 60
           M   LL HP +LPR+ ++  +  SF+ +    P       KIGKCKQ+GLR+VC  +A  
Sbjct: 1   MECVLLRHPVMLPRYKSVAPTRWSFSGVVSVAPPVANLTLKIGKCKQKGLRYVCLCSAAG 60

Query: 61  ----DLFDDNLAAKNGNGAAAAAAAAAAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSI 120
               +LF ++  A+  N     +   +  LG+  E + +  DF DDEDDPD PS+G+SS+
Sbjct: 61  LSAGNLFGESSHARKNN-IGHESVGVSTNLGVNPEISFEGDDFVDDEDDPDRPSDGFSSV 120

Query: 121 QNAVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLE 180
           Q AVDAI  GKFV+VVDDEN D+EGNLVMAAS  + +NITTMVQ  SG++YV MK DDLE
Sbjct: 121 QTAVDAIRHGKFVMVVDDENGDSEGNLVMAASKISATNITTMVQQSSGMVYVAMKGDDLE 180

Query: 181 RLKLPIISI-DNGN-ETSTFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPG 240
           RL LP+IS+ DNGN ETSTFTITVD K  TS GVS ADRAKTIVALSSPESK EDFRRPG
Sbjct: 181 RLNLPLISLLDNGNYETSTFTITVDAKMETSTGVSAADRAKTIVALSSPESKPEDFRRPG 240

Query: 241 HVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKLRKLASE 300
           HVFP+RYRDGGVLRRAGHTEASVDLV+LAGLRPVSVLS IVD  DGSMASL KLR LAS+
Sbjct: 241 HVFPVRYRDGGVLRRAGHTEASVDLVALAGLRPVSVLSPIVDAHDGSMASLSKLRSLASQ 300

Query: 301 KNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIG 360
            +IPIVSITDLIRYRRKRENLIERI++SRLPTKWG+F A+CYRS LDGIEHVA+VKGNI 
Sbjct: 301 HDIPIVSITDLIRYRRKRENLIERISVSRLPTKWGKFQAYCYRSKLDGIEHVAIVKGNIA 360

Query: 361 DGQDVLVRVHSECLTGDIFASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGP 420
           +GQDVLVRVHSECLTGDIFASARCDCGNQLDLAMQMIEKAGRG VIYLRGHEGRGIGLG 
Sbjct: 361 EGQDVLVRVHSECLTGDIFASARCDCGNQLDLAMQMIEKAGRGVVIYLRGHEGRGIGLGA 420

Query: 421 KLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPSKFKGLKG 480
           KLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIG+RTMRLMTNNP+K KGLKG
Sbjct: 421 KLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIGVRTMRLMTNNPAKLKGLKG 480

Query: 481 YGLAVTARVPVVTPITDENRKYLETKRTKMGHIYAKGQF 501
           YGLA+T RVPVVTPITDENRKYLETKRTKMGH+YAK  F
Sbjct: 481 YGLAMTGRVPVVTPITDENRKYLETKRTKMGHVYAKSPF 518

BLAST of Csor.00g192780 vs. ExPASy TrEMBL
Match: A0A7N2KPF3 (GTP cyclohydrolase II OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 666 bits (1718), Expect = 2.32e-235
Identity = 357/515 (69.32%), Postives = 413/515 (80.19%), Query Frame = 0

Query: 1   MASTLLSHPLLPR-FTNLPLSSFNRLSLPHPKIGKC--KQRGLRFVCSAAA-------DL 60
           M S L +HPL    FT+   SS++   +   + G C  K R L   C AA        +L
Sbjct: 1   MDSALFTHPLFSHIFTS---SSWHPSLVAFQRAGICLYKHRLLNRPCWAAGVPGIGAKNL 60

Query: 61  FDDNLAAKNGNGAAAAA---AAAAAALG-LEAETTPDVVDFF--DDEDDPDCPSEGYSSI 120
           FDD+    + NG    A     A+A  G L+A+ TP+ +DFF  D E DPDCPSEGY SI
Sbjct: 61  FDDSSLKGSENGCVLGAFDDELASAPFGTLDAKITPETIDFFVSDAEGDPDCPSEGYFSI 120

Query: 121 QNAVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLE 180
           + A++ + QGKFVIVVDDEN D EGNLVMAAS T+P ++  MV+HGSGI+ VGMK++DL+
Sbjct: 121 EQALNTLRQGKFVIVVDDENGDIEGNLVMAASLTSPMDVAFMVKHGSGIVSVGMKDEDLQ 180

Query: 181 RLKLPIISIDNGNETS---TFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRP 240
           RLKLP++S +  +E S   TFTITVD K GTS GVS +DRAKT++ALSS ESK EDFRRP
Sbjct: 181 RLKLPLMSPETEDEDSSAPTFTITVDAKTGTSTGVSASDRAKTVLALSSLESKPEDFRRP 240

Query: 241 GHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKLRKLAS 300
           GHVFPL+YR+GGVLRRAGHTEASVDLVSLAGLRPVSVLS IVD +DGSMASLP LRKLA 
Sbjct: 241 GHVFPLKYRNGGVLRRAGHTEASVDLVSLAGLRPVSVLSAIVDADDGSMASLPGLRKLAL 300

Query: 301 EKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNI 360
           E NIPIVSITDLIRYRRKRENL+ER AISRLPTKWG F A+CYRS LDG EH+A+VKGNI
Sbjct: 301 EHNIPIVSITDLIRYRRKRENLVERTAISRLPTKWGLFQAYCYRSKLDGTEHIAIVKGNI 360

Query: 361 GDGQDVLVRVHSECLTGDIFASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLG 420
           G GQDVLVRVHSECLTGDI  SARCDCGNQLDLAM++IE+AGRG V+YLRGHEGRGIGLG
Sbjct: 361 GHGQDVLVRVHSECLTGDILGSARCDCGNQLDLAMKLIEQAGRGVVVYLRGHEGRGIGLG 420

Query: 421 PKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPSKFKGLK 480
            KL AYNLQDQGHDTV+ANIELGL  DAR+YG+GAQ+LRD+G++TMRLMTNNP+KF GLK
Sbjct: 421 HKLRAYNLQDQGHDTVQANIELGLAVDAREYGIGAQILRDVGVQTMRLMTNNPAKFTGLK 480

Query: 481 GYGLAVTARVPVVTPITDENRKYLETKRTKMGHIY 496
           GYGLAV  RVPV+TPIT+EN+KYLETKRTKMGH+Y
Sbjct: 481 GYGLAVIGRVPVLTPITEENKKYLETKRTKMGHVY 512

BLAST of Csor.00g192780 vs. TAIR 10
Match: AT5G59750.1 (DHBP synthase RibB-like alpha/beta domain;GTP cyclohydrolase II )

HSP 1 Score: 627.1 bits (1616), Expect = 1.3e-179
Identity = 311/453 (68.65%), Postives = 380/453 (83.89%), Query Frame = 0

Query: 49  DLFDDNLAAKNGNGAAAAAAAAAAALGLEAETTPDVVDFF--DDEDDPDCPSEGYSSIQN 108
           ++FD+N   K       +A+       ++AE TP+  DFF  D E DPDCP++GYSSI+ 
Sbjct: 51  NVFDENPVRKTDGSLFDSASFET----VDAEITPETDDFFVSDAEGDPDCPTQGYSSIEL 110

Query: 109 AVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERL 168
           A+ A+ +GKFVIVVDDE  D EGNL+MAA+ T+P +I  ++++GSGI+ VGMK+++LERL
Sbjct: 111 ALQALRKGKFVIVVDDETGDVEGNLIMAATLTSPKDIAFLIKNGSGIVSVGMKKENLERL 170

Query: 169 KLPIISIDNGNETS---TFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGH 228
            L ++S +  +E S   TFTITVD K+GTS GVS +DRA T++ALSS ++K +DFRRPGH
Sbjct: 171 SLTLMSPEMEDEDSSAPTFTITVDAKSGTSTGVSASDRAMTVLALSSLDAKPDDFRRPGH 230

Query: 229 VFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKLRKLASEK 288
           VFPL+YRDGGVLRRAGHTEASVDL+ LAGLRP+SVLS I+D EDGSMASLP ++KLA+E 
Sbjct: 231 VFPLKYRDGGVLRRAGHTEASVDLMILAGLRPLSVLSAILDQEDGSMASLPYMKKLATEH 290

Query: 289 NIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGD 348
           +IPIVS+TDLIRYRRKR+ L+ERI +SRLPTKWG F A+CYRS LDG E++ALVKGN+G+
Sbjct: 291 DIPIVSLTDLIRYRRKRDKLVERITVSRLPTKWGLFQAYCYRSKLDGTENIALVKGNVGN 350

Query: 349 GQDVLVRVHSECLTGDIFASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPK 408
           G+D+LVRVHSECLTGDIF SARCDCGNQLDLAM++IEK GRG V+YLRGHEGRGIGLG K
Sbjct: 351 GEDILVRVHSECLTGDIFGSARCDCGNQLDLAMELIEKEGRGVVVYLRGHEGRGIGLGHK 410

Query: 409 LLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPSKFKGLKGY 468
           L AYNLQD+GHDTV+AN+ELGL  D+R+YG+GAQMLRDIG+RTMRLMTNNP+KF GLKGY
Sbjct: 411 LRAYNLQDEGHDTVQANVELGLSIDSREYGIGAQMLRDIGVRTMRLMTNNPAKFTGLKGY 470

Query: 469 GLAVTARVPVVTPITDENRKYLETKRTKMGHIY 497
           GLAV  RVPVVTPIT ENR+Y+ETKR KMGHIY
Sbjct: 471 GLAVVGRVPVVTPITKENRRYMETKRKKMGHIY 499

BLAST of Csor.00g192780 vs. TAIR 10
Match: AT5G59750.2 (DHBP synthase RibB-like alpha/beta domain;GTP cyclohydrolase II )

HSP 1 Score: 609.8 bits (1571), Expect = 2.1e-174
Identity = 311/487 (63.86%), Postives = 380/487 (78.03%), Query Frame = 0

Query: 49  DLFDDNLAAKNGNGAAAAAAAAAAALGLEAETTPDVVDFF--DDEDDPDCPSEGYSSIQN 108
           ++FD+N   K       +A+       ++AE TP+  DFF  D E DPDCP++GYSSI+ 
Sbjct: 51  NVFDENPVRKTDGSLFDSASFET----VDAEITPETDDFFVSDAEGDPDCPTQGYSSIEL 110

Query: 109 AVDAISQGK----------------------------------FVIVVDDENEDTEGNLV 168
           A+ A+ +GK                                  FVIVVDDE  D EGNL+
Sbjct: 111 ALQALRKGKVCSLTIFLKTSDYDDLFLQLYRFGLTKKLSLMFQFVIVVDDETGDVEGNLI 170

Query: 169 MAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETS---TFTITVDLK 228
           MAA+ T+P +I  ++++GSGI+ VGMK+++LERL L ++S +  +E S   TFTITVD K
Sbjct: 171 MAATLTSPKDIAFLIKNGSGIVSVGMKKENLERLSLTLMSPEMEDEDSSAPTFTITVDAK 230

Query: 229 NGTSAGVSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVS 288
           +GTS GVS +DRA T++ALSS ++K +DFRRPGHVFPL+YRDGGVLRRAGHTEASVDL+ 
Sbjct: 231 SGTSTGVSASDRAMTVLALSSLDAKPDDFRRPGHVFPLKYRDGGVLRRAGHTEASVDLMI 290

Query: 289 LAGLRPVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKRENLIERIAI 348
           LAGLRP+SVLS I+D EDGSMASLP ++KLA+E +IPIVS+TDLIRYRRKR+ L+ERI +
Sbjct: 291 LAGLRPLSVLSAILDQEDGSMASLPYMKKLATEHDIPIVSLTDLIRYRRKRDKLVERITV 350

Query: 349 SRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIFASARCDCG 408
           SRLPTKWG F A+CYRS LDG E++ALVKGN+G+G+D+LVRVHSECLTGDIF SARCDCG
Sbjct: 351 SRLPTKWGLFQAYCYRSKLDGTENIALVKGNVGNGEDILVRVHSECLTGDIFGSARCDCG 410

Query: 409 NQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADA 468
           NQLDLAM++IEK GRG V+YLRGHEGRGIGLG KL AYNLQD+GHDTV+AN+ELGL  D+
Sbjct: 411 NQLDLAMELIEKEGRGVVVYLRGHEGRGIGLGHKLRAYNLQDEGHDTVQANVELGLSIDS 470

Query: 469 RDYGVGAQMLRDIGIRTMRLMTNNPSKFKGLKGYGLAVTARVPVVTPITDENRKYLETKR 497
           R+YG+GAQMLRDIG+RTMRLMTNNP+KF GLKGYGLAV  RVPVVTPIT ENR+Y+ETKR
Sbjct: 471 REYGIGAQMLRDIGVRTMRLMTNNPAKFTGLKGYGLAVVGRVPVVTPITKENRRYMETKR 530

BLAST of Csor.00g192780 vs. TAIR 10
Match: AT5G64300.1 (GTP cyclohydrolase II )

HSP 1 Score: 560.8 bits (1444), Expect = 1.1e-159
Identity = 305/535 (57.01%), Postives = 386/535 (72.15%), Query Frame = 0

Query: 2   ASTLLSHPL----LPRFTNLPLSSFNRLSLPHPKIGKCKQRGLRFVCSAAADLFDDNLAA 61
           +ST L H L    LP  +N PLS+F+  +      GK K        +A     DD L+ 
Sbjct: 24  SSTTLLHGLHRVTLP--SNHPLSTFSIKT----NTGKVK--------AAVISREDDLLSF 83

Query: 62  KNGNGAAAAAA-----------AAAAALG-LEAETTPDVVDFFDDEDDP---DCPSEGYS 121
            NGN   +  +           A + +LG L A++ P   + F  EDD    D P+ G+S
Sbjct: 84  TNGNTPLSNGSLIDDRTEEPLEADSVSLGTLAADSAPAPANGFVAEDDDFELDLPTPGFS 143

Query: 122 SIQNAVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDD 181
           SI  A++ I QGK V+VVDDE+ + EG+LVMAA   TP  +  +V+HG+GI+ V MKEDD
Sbjct: 144 SIPEAIEDIRQGKLVVVVDDEDRENEGDLVMAAQLATPEAMAFIVRHGTGIVCVSMKEDD 203

Query: 182 LERLKLPIISIDNGNE---TSTFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFR 241
           LERL LP++     NE   ++ FT+TVD K+GT+ GVS  DRA TI++L+S +SK EDF 
Sbjct: 204 LERLHLPLMVNQKENEEKLSTAFTVTVDAKHGTTTGVSARDRATTILSLASRDSKPEDFN 263

Query: 242 RPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKLRKL 301
           RPGH+FPL+YR+GGVL+RAGHTEASVDL  LAGL PV VL  IVD +DGSMA LPKLR+ 
Sbjct: 264 RPGHIFPLKYREGGVLKRAGHTEASVDLTVLAGLDPVGVLCEIVD-DDGSMARLPKLREF 323

Query: 302 ASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKG 361
           A+E N+ +VSI DLIRYRRKR+ L+ER + +R+PT WG F A+CYRS LDGIEH+A+VKG
Sbjct: 324 AAENNLKVVSIADLIRYRRKRDKLVERASAARIPTMWGPFTAYCYRSILDGIEHIAMVKG 383

Query: 362 NIGDGQDVLVRVHSECLTGDIFASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIG 421
            IGDGQD+LVRVHSECLTGDIF SARCDCGNQL L+MQ IE  GRG ++YLRGHEGRGIG
Sbjct: 384 EIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALSMQQIEATGRGVLVYLRGHEGRGIG 443

Query: 422 LGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPSKFKG 481
           LG KL AYNLQD G DTVEAN ELGLP D+R+YG+GAQ++RD+G+RTM+LMTNNP+K+ G
Sbjct: 444 LGHKLRAYNLQDAGRDTVEANEELGLPVDSREYGIGAQIIRDLGVRTMKLMTNNPAKYVG 503

Query: 482 LKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHIYA---KGQFLRSRQAEAST 512
           LKGYGLA+  RVP+++ IT EN++YLETKRTKMGH+Y    KG  +   ++E+ +
Sbjct: 504 LKGYGLAIVGRVPLLSLITKENKRYLETKRTKMGHMYGLKFKGDVVEKIESESES 543

BLAST of Csor.00g192780 vs. TAIR 10
Match: AT2G22450.1 (riboflavin biosynthesis protein, putative )

HSP 1 Score: 434.9 bits (1117), Expect = 9.1e-122
Identity = 233/457 (50.98%), Postives = 315/457 (68.93%), Query Frame = 0

Query: 26  SLPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNGNGAAAAAAAAAAALG-LEAE---TT 85
           SL +P+I   K      V     D+F    +  NG+           + G L AE   TT
Sbjct: 29  SLVYPRIISKKFNVRMRVIPEEGDVFSS--SKSNGSSMGIELQPDLVSFGTLAAEMIPTT 88

Query: 86  PDVVDFFDDEDDPDCPSEGYSSIQNAVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPS 145
            D  +  D+E D D P++G++SI  A++ I  GK V+VVDDE+ + EG+L+MAAS  TP 
Sbjct: 89  MDSPEVEDEEFDLDRPTDGFASIPQAIEDIRHGKMVVVVDDEDRENEGDLIMAASLATPE 148

Query: 146 NITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNE---TSTFTITVDLKNGTSAGVST 205
            +  +V+HG+GI+ V MK +DLERL+LP++     NE    + FT++VD K GTS GVS 
Sbjct: 149 AMAFVVKHGTGIVCVSMKGEDLERLELPLMVTRKDNEEKLRTAFTVSVDAKKGTSTGVSA 208

Query: 206 ADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSV 265
            DRA+TI+ L+S +SK EDF RPGH+FPLRYR+GGVL+RAGHTEASVDL  LAGL PVSV
Sbjct: 209 RDRAQTILTLASKDSKPEDFNRPGHIFPLRYREGGVLKRAGHTEASVDLTVLAGLEPVSV 268

Query: 266 LSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQ 325
           L  IVD +DGSMA LP+LR+ A E N+ ++SI DLIRYRRKRE L+E  A++ +PT WG 
Sbjct: 269 LCEIVD-DDGSMARLPRLRQFAQENNLKLISIADLIRYRRKRERLVEFTAVAPIPTMWGP 328

Query: 326 FYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIFASARCDCGNQLDLAMQM 385
           F AHC++S LDG+EH+A+VKG IGDG+D+LVRVH+EC+T DIF ++    G QL +AM++
Sbjct: 329 FKAHCFKSLLDGVEHIAMVKGEIGDGKDILVRVHAECITDDIFGNS--SGGKQLAIAMRL 388

Query: 386 IEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQM 445
           IE+ GRG  +YLRG E +GI L  K   YN      D  E    +  P  +R+YG+GAQ+
Sbjct: 389 IEENGRGVFVYLRGPESKGIDLSHKPRTYNTNS---DQAEG---VSFPVASREYGIGAQI 448

Query: 446 LRDIGIRTMRLMTNNPSKFKGLKGYGLAVTARVPVVT 476
           LRD+G+R M++MTNNP+ + GLKGYGL+++ +VP++T
Sbjct: 449 LRDLGVREMKVMTNNPAHYVGLKGYGLSISGKVPLIT 474

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FN891.8e-17868.65Monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic OS=Arabidop... [more]
Q6L5064.1e-16764.76Probable monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic OS... [more]
Q6Z2349.4e-16459.23Probable bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic OS=O... [more]
Q0E0795.1e-16266.04Probable bifunctional riboflavin biosynthesis protein RIBA 2, chloroplastic OS=O... [more]
P479241.5e-15857.01Bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic OS=Arabidopsi... [more]
Match NameE-valueIdentityDescription
KAG6607228.10.0100.00Monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic, partial [C... [more]
XP_022948312.10.099.02monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic-like [Cucur... [more]
XP_023522983.10.098.05monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic-like [Cucur... [more]
XP_022998776.10.096.68monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic-like [Cucur... [more]
XP_022159223.12.01e-28281.10monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic-like [Momor... [more]
Match NameE-valueIdentityDescription
A0A6J1G8U40.099.02GTP cyclohydrolase II OS=Cucurbita moschata OX=3662 GN=LOC111452026 PE=3 SV=1[more]
A0A6J1KB550.096.68GTP cyclohydrolase II OS=Cucurbita maxima OX=3661 GN=LOC111493343 PE=3 SV=1[more]
A0A6J1DY859.72e-28381.10GTP cyclohydrolase II OS=Momordica charantia OX=3673 GN=LOC111025642 PE=3 SV=1[more]
A0A6J1DXS13.56e-25774.57GTP cyclohydrolase II OS=Momordica charantia OX=3673 GN=LOC111024475 PE=3 SV=1[more]
A0A7N2KPF32.32e-23569.32GTP cyclohydrolase II OS=Quercus lobata OX=97700 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G59750.11.3e-17968.65DHBP synthase RibB-like alpha/beta domain;GTP cyclohydrolase II [more]
AT5G59750.22.1e-17463.86DHBP synthase RibB-like alpha/beta domain;GTP cyclohydrolase II [more]
AT5G64300.11.1e-15957.01GTP cyclohydrolase II [more]
AT2G22450.19.1e-12250.98riboflavin biosynthesis protein, putative [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0004223,4-dihydroxy-2-butanone 4-phosphate synthase, RibBTIGRFAMTIGR00506TIGR00506coord: 103..297
e-value: 2.6E-57
score: 191.6
IPR0004223,4-dihydroxy-2-butanone 4-phosphate synthase, RibBPFAMPF00926DHBP_synthasecoord: 106..296
e-value: 2.0E-68
score: 229.7
NoneNo IPR availableGENE3D3.90.870.10DHBP synthasecoord: 94..303
e-value: 7.1E-76
score: 256.1
NoneNo IPR availablePANTHERPTHR21327:SF29MONOFUNCTIONAL RIBOFLAVIN BIOSYNTHESIS PROTEIN RIBA 3, CHLOROPLASTICcoord: 47..497
NoneNo IPR availablePANTHERPTHR21327GTP CYCLOHYDROLASE II-RELATEDcoord: 47..497
IPR036144GTP cyclohydrolase II superfamilyGENE3D3.40.50.10990GTP cyclohydrolase IIcoord: 304..475
e-value: 1.0E-76
score: 258.1
IPR036144GTP cyclohydrolase II superfamilySUPERFAMILY142695RibA-likecoord: 303..474
IPR000926GTP cyclohydrolase II, RibATIGRFAMTIGR00505TIGR00505coord: 310..495
e-value: 1.0E-74
score: 248.2
IPR000926GTP cyclohydrolase II, RibAHAMAPMF_00179RibAcoord: 302..498
score: 40.06826
IPR000926GTP cyclohydrolase II, RibACDDcd00641GTP_cyclohydro2coord: 303..495
e-value: 2.55021E-117
score: 341.787
IPR032677GTP cyclohydrolase IIPFAMPF00925GTP_cyclohydro2coord: 309..472
e-value: 3.3E-71
score: 238.2
IPR016299Riboflavin biosynthesis protein RibBAHAMAPMF_01283RibBAcoord: 100..494
score: 49.245045
IPR017945DHBP synthase RibB-like alpha/beta domain superfamilySUPERFAMILY55821YrdC/RibBcoord: 100..301

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g192780.m01Csor.00g192780.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009231 riboflavin biosynthetic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0008686 3,4-dihydroxy-2-butanone-4-phosphate synthase activity
molecular_function GO:0005525 GTP binding
molecular_function GO:0003935 GTP cyclohydrolase II activity
molecular_function GO:0046872 metal ion binding