Csor.00g189640 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g189640
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionshort-chain dehydrogenase TIC 32, chloroplastic-like
LocationCsor_Chr16: 9058477 .. 9059990 (-)
RNA-Seq ExpressionCsor.00g189640
SyntenyCsor.00g189640
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTTGAAATGTTCAAGTACCTCGTCGGTTCCGCTGGAGCAAGCGGCTACGGCTCTAAATCCACCGCCGAAGAAGTCAGTGAATCTTGTATGAACTCCATCACCGCCATAGTCACAGGTTGTTTTTCCGAATTTTGATTCTGATTCCATTACATTTCATTAGATTCTCACTCGCTAACGAATTTTGGAGCAGGAGCGACGTCGGGAATCGGAGAGGAGACGGCGAGAGTCTTGGCCAAGCGCGGTGTAAGGATCATCATTCCGGCTCGAAATCTAAAAGCCGCTGAGGAAGCGAAGGCGAGAATCGTATCCGAATCGGATTGTTTTGATCATTCACGAATCACGGTTATGGCTCTCGATCTTAGCTCTGTGAATTCCGTCGTGAACTTCGTCTCCAATTTCGAGTCGTTAAATTTGCCTCTTAATCTTCTCATGTAATTCACTCAAACACGTCGCTAATTCACTCTGTTGTATCGAATGATCACACGATATTTAATTTACCGAAGAAACTAAAACTCAGTTTCGTTCTAATTTTCAGAAACAACGCAGGCAGGTTCTGTTACGAACATGCGTTTTCTGAAGACGGAATTGAGATGACTTTCGCTACTAATTATCTTGGCAAGAACATTACAGAACAATTTTATTTTCATCTCCTTTAATCACGAGGCCTTTTACCATTATTGACGAACCGATGATGCAGGTCATTTTCTCTTGACGAAACTTCTATTGAATAGGATGATAGAAACCGCCAAATCGACAGGAGTCCAAGGCCGTATAGTGAATGTGACGTCCAACATCCATAGCTGGTTTTCGGGCGACATATTCGAATATCTCGGCCAGATAAGCCAGAAAAACAGGTATGAATAATGATTAATTTCCCTTCGCTTATGCATTACTGGCGGTTTGATCATAGCGACAGTGATATTCACTTGCAGCAGAGTGTACGATTCGACACGTGCTTACGCGTTCTCCAAACTCGCAAATGTCTTGCACACTGTTCAACTCGCTCACAAACTCCAGGTTCTTCAATACATCACATTCATTGGCATTTTAATTTAATGATTTCACGTCCGCGTTGGATTTTGACACAATCGAAACAGGAAATGAAGGCGAATGTTACGGTGAACTGTGTTCATCCAGGCGTTGTGAAAACCAATTTGAACAGAGAACGTGAAGGCTTCGTCACAGGTACGAACTACCGAACAATTAAGCTCCTGCATGGAAACTAATCGATCTAAAAAGCGTATACAGATAATTAATTCAATGAGAATGCAGATTTGATTTTCTACATGGCGTCCAAATCAAGGTTGTTGAAGACAATTCCTGAAGGTGCAGCAACAAGTTGTTACGTAGCCACGAATCGGAACGTGGAAAATGTGAATGGAAATTACTTCGCGGATTGCAACGATCAAGGAAGTTGCGAATGCAAAACGAAATCCGCGCATTTGGCTGAAGGCCTATGGTCCGCCTCCGAAACCATCGTCTCCACACTTACCGGTAAGCCTCAACCCTAA

mRNA sequence

ATGCTTGAAATGTTCAAGTACCTCGTCGGTTCCGCTGGAGCAAGCGGCTACGGCTCTAAATCCACCGCCGAAGAAGTCAGTGAATCTTGTATGAACTCCATCACCGCCATAGTCACAGGAGCGACGTCGGGAATCGGAGAGGAGACGGCGAGAGTCTTGGCCAAGCGCGGTGTAAGGATCATCATTCCGGCTCGAAATCTAAAAGCCGCTGAGGAAGCGAAGGCGAGAATCGTATCCGAATCGGATTGTTTTGATCATTCACGAATCACGGTTATGGCTCTCGATCTTAGCTCTGTGAATTCCGTCGTGAACTTCGTCTCCAATTTCGAGTCGTTAAATTTGCCTCTTAATCTTCTCATAAACAACGCAGGCAGGTTCTGTTACGAACATGCGTTTTCTGAAGACGGAATTGAGATGACTTTCGCTACTAATTATCTTGGTCATTTTCTCTTGACGAAACTTCTATTGAATAGGATGATAGAAACCGCCAAATCGACAGGAGTCCAAGGCCGTATAGTGAATGTGACGTCCAACATCCATAGCTGGTTTTCGGGCGACATATTCGAATATCTCGGCCAGATAAGCCAGAAAAACAGCAGAGTGTACGATTCGACACGTGCTTACGCGTTCTCCAAACTCGCAAATGTCTTGCACACTGTTCAACTCGCTCACAAACTCCAGGAAATGAAGGCGAATGTTACGGTGAACTGTGTTCATCCAGGCGTTGTGAAAACCAATTTGAACAGAGAACGTGAAGGCTTCGTCACAGATTTGATTTTCTACATGGCGTCCAAATCAAGGTTGTTGAAGACAATTCCTGAAGGTGCAGCAACAAGTTGTTACGTAGCCACGAATCGGAACGTGGAAAATGTGAATGGAAATTACTTCGCGGATTGCAACGATCAAGGAAGTTGCGAATGCAAAACGAAATCCGCGCATTTGGCTGAAGGCCTATGGTCCGCCTCCGAAACCATCGTCTCCACACTTACCGGTAAGCCTCAACCCTAA

Coding sequence (CDS)

ATGCTTGAAATGTTCAAGTACCTCGTCGGTTCCGCTGGAGCAAGCGGCTACGGCTCTAAATCCACCGCCGAAGAAGTCAGTGAATCTTGTATGAACTCCATCACCGCCATAGTCACAGGAGCGACGTCGGGAATCGGAGAGGAGACGGCGAGAGTCTTGGCCAAGCGCGGTGTAAGGATCATCATTCCGGCTCGAAATCTAAAAGCCGCTGAGGAAGCGAAGGCGAGAATCGTATCCGAATCGGATTGTTTTGATCATTCACGAATCACGGTTATGGCTCTCGATCTTAGCTCTGTGAATTCCGTCGTGAACTTCGTCTCCAATTTCGAGTCGTTAAATTTGCCTCTTAATCTTCTCATAAACAACGCAGGCAGGTTCTGTTACGAACATGCGTTTTCTGAAGACGGAATTGAGATGACTTTCGCTACTAATTATCTTGGTCATTTTCTCTTGACGAAACTTCTATTGAATAGGATGATAGAAACCGCCAAATCGACAGGAGTCCAAGGCCGTATAGTGAATGTGACGTCCAACATCCATAGCTGGTTTTCGGGCGACATATTCGAATATCTCGGCCAGATAAGCCAGAAAAACAGCAGAGTGTACGATTCGACACGTGCTTACGCGTTCTCCAAACTCGCAAATGTCTTGCACACTGTTCAACTCGCTCACAAACTCCAGGAAATGAAGGCGAATGTTACGGTGAACTGTGTTCATCCAGGCGTTGTGAAAACCAATTTGAACAGAGAACGTGAAGGCTTCGTCACAGATTTGATTTTCTACATGGCGTCCAAATCAAGGTTGTTGAAGACAATTCCTGAAGGTGCAGCAACAAGTTGTTACGTAGCCACGAATCGGAACGTGGAAAATGTGAATGGAAATTACTTCGCGGATTGCAACGATCAAGGAAGTTGCGAATGCAAAACGAAATCCGCGCATTTGGCTGAAGGCCTATGGTCCGCCTCCGAAACCATCGTCTCCACACTTACCGGTAAGCCTCAACCCTAA

Protein sequence

MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRIIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLINNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIHSWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCNDQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP
Homology
BLAST of Csor.00g189640 vs. ExPASy Swiss-Prot
Match: A0A078IS66 (Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=TIC32A PE=2 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 3.5e-66
Identity = 149/331 (45.02%), Postives = 209/331 (63.14%), Query Frame = 0

Query: 2   LEMFKYLVGSAGASGYGSKSTAEEVSESC-MNSITAIVTGATSGIGEETARVLAKRGVRI 61
           +E++  + G AG SGYGS STAE+V+ S     +TAI+TG TSGIG E ARVL  RG  +
Sbjct: 1   MEIYGMVTGKAGKSGYGSASTAEDVTHSIDAKHLTAIITGGTSGIGLEAARVLGMRGAHV 60

Query: 62  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 121
           II +RN KAA ++K  I+     + ++RI  + LDLSS+ SV +F+  F +LN+PLN+LI
Sbjct: 61  IIASRNTKAANDSKEMIL---QMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILI 120

Query: 122 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 181
           NNAG        SEDGIE  FATN++GHFLLT LLL++M  +A+ +G++GRIVN++S  H
Sbjct: 121 NNAGVMFCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAH 180

Query: 182 --SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCV 241
             ++  G +F+Y+    +     Y   +AY  SKLAN+LH+  L+ KLQE   N+T+N V
Sbjct: 181 TYTYTEGIMFDYINDPDR-----YSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSV 240

Query: 242 HPGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFAD 301
           HPG++ TNL R        +    A    L K IP+GAAT+CYVA + ++++V G YFAD
Sbjct: 241 HPGLITTNLFRHSG---LGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKDVTGKYFAD 300

Query: 302 CNDQGSCECKTKSAHLAEGLWSASETIVSTL 330
           CN          +  LA+ LW  S  +V +L
Sbjct: 301 CNVTTPSNFAIDTT-LADKLWDFSIKLVESL 319

BLAST of Csor.00g189640 vs. ExPASy Swiss-Prot
Match: A0A078ISJ6 (Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=TIC32B PE=3 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 5.9e-66
Identity = 149/331 (45.02%), Postives = 207/331 (62.54%), Query Frame = 0

Query: 2   LEMFKYLVGSAGASGYGSKSTAEEVSESC-MNSITAIVTGATSGIGEETARVLAKRGVRI 61
           +E++  + G AG SGYGS STAE+V+ S     +TAI+TG TSGIG E ARVL  RG  +
Sbjct: 1   MEIYGMVTGKAGKSGYGSASTAEDVTHSIDAKHLTAIITGGTSGIGLEAARVLGMRGAHV 60

Query: 62  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 121
           II ARN KAA ++K  I+     + ++RI  + LDLSS+ SV +F+  F +LN+PLN+LI
Sbjct: 61  IIAARNTKAANDSKEMIL---QMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILI 120

Query: 122 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 181
           NNAG        SEDGIE  FATN++GHFLLT LLL++M  +A+ +G++GRIVN++S  H
Sbjct: 121 NNAGVMFCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAH 180

Query: 182 --SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCV 241
             ++  G +F+Y+    +     Y   +AY  SKLAN+LH+  L+ KLQE   N+T+N V
Sbjct: 181 TYTYTEGIMFDYINDPDR-----YSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSV 240

Query: 242 HPGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFAD 301
           HPG++ TNL R        +    A    L K IP+GAAT+CYVA + +++ V G YF D
Sbjct: 241 HPGLITTNLFRHSG---LGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKGVTGKYFTD 300

Query: 302 CNDQGSCECKTKSAHLAEGLWSASETIVSTL 330
           CN          +  LA+ LW  S  +V +L
Sbjct: 301 CNVTTPSNFAIDTT-LADKLWDFSIKLVDSL 319

BLAST of Csor.00g189640 vs. ExPASy Swiss-Prot
Match: A2RVM0 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC32 PE=2 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 9.8e-61
Identity = 146/329 (44.38%), Postives = 203/329 (61.70%), Query Frame = 0

Query: 7   YLVGSAGASGYGSKSTAEEVSESCMNS-ITAIVTGATSGIGEETARVLAKRGVRIIIPAR 66
           +  GS GASG+ S+STAEEV+     + +TAIVTGA+SGIG ETARVL+ RGV +++  R
Sbjct: 2   WFFGSKGASGFSSRSTAEEVTHGVDGTGLTAIVTGASSGIGVETARVLSLRGVHVVMAVR 61

Query: 67  NLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLINNAGR 126
           N  +  + K  IV +      +++ VM LDLSS+ SV  F S ++S  LPLNLLINNAG 
Sbjct: 62  NTDSGAKVKEDIVKQ---VPGAKLDVMELDLSSMQSVRKFASEYKSTGLPLNLLINNAGI 121

Query: 127 FCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH--SWF 186
                  S+D IE+ FATN+LGHFLLTKLLL+ M  T++ +  +GRIVN++S  H  S+ 
Sbjct: 122 MACPFMLSKDNIELQFATNHLGHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHRFSYP 181

Query: 187 SGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPGVV 246
            G  F+   +I+ K+S  Y S RAY  SKL NVLH  +L  +L+E   N+T N +HPG +
Sbjct: 182 EGVRFD---KINDKSS--YSSMRAYGQSKLCNVLHANELTKQLKEDGVNITANSLHPGAI 241

Query: 247 KTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCNDQG 306
            TNL R    ++   +  +A    +LK++P+GAAT+CYVA N  V  V+G YF D N   
Sbjct: 242 MTNLGRYFNPYLAVAVGAVA--KYILKSVPQGAATTCYVALNPQVAGVSGEYFQDSNIAK 301

Query: 307 SCECKTKSAHLAEGLWSASETIVSTLTGK 333
                 K   LA+ +W  S  +  + +G+
Sbjct: 302 PLPL-VKDTELAKKVWDFSTKLTDSQSGE 319

BLAST of Csor.00g189640 vs. ExPASy Swiss-Prot
Match: Q6RVV4 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC32 PE=1 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 4.7e-55
Identity = 136/315 (43.17%), Postives = 189/315 (60.00%), Query Frame = 0

Query: 13  GASGYGSKSTAEEVSESC-MNSITAIVTGATSGIGEETARVLAKRGVRIIIPARNLKAAE 72
           G SG+   STAE+V+       +TAIVTGA+SGIG ET RVLA RG  +I+  RN+ AA+
Sbjct: 9   GVSGFSGSSTAEQVTHGIDATGLTAIVTGASSGIGAETTRVLALRGAHVIMGVRNMVAAK 68

Query: 73  EAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLINNAGRFCYEHA 132
           + K  I+ +      +++  + LDLSS++SV  F S F S   PLN+LINNAG       
Sbjct: 69  DVKDTILKD---IPSAKVDAIELDLSSLDSVKKFASEFNSSGRPLNILINNAGIMACPFK 128

Query: 133 FSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIHSWFSGDIFEYL 192
            S+D IE+ FATN++GHFLLT LLL+ M +T + +  +GRIVNV S  H +   +   + 
Sbjct: 129 LSKDNIELQFATNHIGHFLLTNLLLDTMKKTTRESKKEGRIVNVASEAHRFAYPEGIRF- 188

Query: 193 GQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPGVVKTNLNRER 252
            +I+ ++S  Y++ RAY  SKLANVLH  QL   L+E   N+T N +HPG + TNL R  
Sbjct: 189 DKINDQSS--YNNWRAYGQSKLANVLHANQLTKHLKEDGVNITANSLHPGTIVTNLFRHN 248

Query: 253 EGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCNDQGSCECKTKS 312
              V  LI  +     +LK + +GAAT+CYVA +  V+ V+G YF+D N   +     K 
Sbjct: 249 SA-VNGLINVIG--KLVLKNVQQGAATTCYVALHPQVKGVSGEYFSDSNVYKTTP-HGKD 308

Query: 313 AHLAEGLWSASETIV 327
             LA+ LW  S  +V
Sbjct: 309 VDLAKKLWDFSINLV 313

BLAST of Csor.00g189640 vs. ExPASy Swiss-Prot
Match: Q9HBH5 (Retinol dehydrogenase 14 OS=Homo sapiens OX=9606 GN=RDH14 PE=1 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 8.9e-38
Identity = 118/311 (37.94%), Postives = 169/311 (54.34%), Query Frame = 0

Query: 31  MNSITAIVTGATSGIGEETARVLAKRGVRIIIPARNLKAAEEAKARIVSE----SDCFDH 90
           M+  T ++TGA SG+G  TA  L + G R+I+  R+   AEEA  ++  E    ++C   
Sbjct: 41  MHGKTVLITGANSGLGRATAAELLRLGARVIMGCRDRARAEEAAGQLRRELRQAAECGPE 100

Query: 91  ------SRITVMALDLSSVNSVVNFVSNFESLNLPLNLLINNAGRFCYEHAFSEDGIEMT 150
                   + V  LDL+S+ SV  F          L++LINNAG F   +  +EDG EM 
Sbjct: 101 PGVSGVGELIVRELDLASLRSVRAFCQEMLQEEPRLDVLINNAGIFQCPYMKTEDGFEMQ 160

Query: 151 FATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIHSWFSGDI-FEYLGQISQKNS 210
           F  N+LGHFLLT LLL  +  +A S     RIV V+S ++ +  GDI F+ L      + 
Sbjct: 161 FGVNHLGHFLLTNLLLGLLKSSAPS-----RIVVVSSKLYKY--GDINFDDL-----NSE 220

Query: 211 RVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPGVVKTNLNRE-REGFVTDL 270
           + Y+ +  Y+ SKLAN+L T +LA +L+    NVTVN +HPG+V+TNL R      +   
Sbjct: 221 QSYNKSFCYSRSKLANILFTRELARRLE--GTNVTVNVLHPGIVRTNLGRHIHIPLLVKP 280

Query: 271 IFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCNDQGSCECKTKSAHLAEGL 330
           +F + S +   KT  EGA TS Y+A++  VE V+G YF DC ++     K     +A  L
Sbjct: 281 LFNLVSWA-FFKTPVEGAQTSIYLASSPEVEGVSGRYFGDCKEEELLP-KAMDESVARKL 335

BLAST of Csor.00g189640 vs. NCBI nr
Match: XP_022923250.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita moschata] >KAG6577763.1 Short-chain dehydrogenase TIC 32 A, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 657 bits (1695), Expect = 9.05e-238
Identity = 335/335 (100.00%), Postives = 335/335 (100.00%), Query Frame = 0

Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60
           MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60

Query: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120
           IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI
Sbjct: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120

Query: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180
           NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH
Sbjct: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180

Query: 181 SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240
           SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP
Sbjct: 181 SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240

Query: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 300
           GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN
Sbjct: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 300

Query: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335
           DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP
Sbjct: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335

BLAST of Csor.00g189640 vs. NCBI nr
Match: XP_023552711.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 654 bits (1688), Expect = 1.06e-236
Identity = 333/335 (99.40%), Postives = 334/335 (99.70%), Query Frame = 0

Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60
           MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60

Query: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120
           IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI
Sbjct: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120

Query: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180
           NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH
Sbjct: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180

Query: 181 SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240
           SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP
Sbjct: 181 SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240

Query: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 300
           GVVKTNLNREREGFVTDLIFYM SKSRLLKTIPEGAAT+CYVATNRNVENVNGNYFADCN
Sbjct: 241 GVVKTNLNREREGFVTDLIFYMTSKSRLLKTIPEGAATTCYVATNRNVENVNGNYFADCN 300

Query: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335
           DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP
Sbjct: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335

BLAST of Csor.00g189640 vs. NCBI nr
Match: XP_022923251.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita moschata] >KAG7015802.1 Short-chain dehydrogenase TIC 32, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 651 bits (1679), Expect = 2.40e-235
Identity = 334/335 (99.70%), Postives = 334/335 (99.70%), Query Frame = 0

Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60
           MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60

Query: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120
           IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI
Sbjct: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120

Query: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180
           NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH
Sbjct: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180

Query: 181 SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240
           SWFSGDIFEYLGQISQKN RVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP
Sbjct: 181 SWFSGDIFEYLGQISQKN-RVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240

Query: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 300
           GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN
Sbjct: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 300

Query: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335
           DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP
Sbjct: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 334

BLAST of Csor.00g189640 vs. NCBI nr
Match: XP_023552712.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 648 bits (1672), Expect = 2.80e-234
Identity = 332/335 (99.10%), Postives = 333/335 (99.40%), Query Frame = 0

Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60
           MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60

Query: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120
           IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI
Sbjct: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120

Query: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180
           NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH
Sbjct: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180

Query: 181 SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240
           SWFSGDIFEYLGQISQKN RVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP
Sbjct: 181 SWFSGDIFEYLGQISQKN-RVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240

Query: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 300
           GVVKTNLNREREGFVTDLIFYM SKSRLLKTIPEGAAT+CYVATNRNVENVNGNYFADCN
Sbjct: 241 GVVKTNLNREREGFVTDLIFYMTSKSRLLKTIPEGAATTCYVATNRNVENVNGNYFADCN 300

Query: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335
           DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP
Sbjct: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 334

BLAST of Csor.00g189640 vs. NCBI nr
Match: XP_022965265.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 643 bits (1658), Expect = 3.95e-232
Identity = 329/335 (98.21%), Postives = 330/335 (98.51%), Query Frame = 0

Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60
           MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60

Query: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120
           IIPARNLKAAEEAKARIVSES+CFD SRITVMALDLSSV SVVNFVSNFESLNLPLNLLI
Sbjct: 61  IIPARNLKAAEEAKARIVSESNCFDRSRITVMALDLSSVKSVVNFVSNFESLNLPLNLLI 120

Query: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180
           NNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH
Sbjct: 121 NNAGRFCYEHALSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180

Query: 181 SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240
           SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP
Sbjct: 181 SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240

Query: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 300
           GVVKTNLNREREGFVTDLIFYMASKSRLLK IPEGAATSCYVATNRNVENVNG YFADCN
Sbjct: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKIIPEGAATSCYVATNRNVENVNGKYFADCN 300

Query: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335
           DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP
Sbjct: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335

BLAST of Csor.00g189640 vs. ExPASy TrEMBL
Match: A0A6J1EB90 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430995 PE=4 SV=1)

HSP 1 Score: 657 bits (1695), Expect = 4.38e-238
Identity = 335/335 (100.00%), Postives = 335/335 (100.00%), Query Frame = 0

Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60
           MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60

Query: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120
           IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI
Sbjct: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120

Query: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180
           NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH
Sbjct: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180

Query: 181 SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240
           SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP
Sbjct: 181 SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240

Query: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 300
           GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN
Sbjct: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 300

Query: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335
           DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP
Sbjct: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335

BLAST of Csor.00g189640 vs. ExPASy TrEMBL
Match: A0A6J1E6B3 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430995 PE=4 SV=1)

HSP 1 Score: 651 bits (1679), Expect = 1.16e-235
Identity = 334/335 (99.70%), Postives = 334/335 (99.70%), Query Frame = 0

Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60
           MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60

Query: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120
           IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI
Sbjct: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120

Query: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180
           NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH
Sbjct: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180

Query: 181 SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240
           SWFSGDIFEYLGQISQKN RVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP
Sbjct: 181 SWFSGDIFEYLGQISQKN-RVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240

Query: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 300
           GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN
Sbjct: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 300

Query: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335
           DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP
Sbjct: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 334

BLAST of Csor.00g189640 vs. ExPASy TrEMBL
Match: A0A6J1HN85 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465182 PE=4 SV=1)

HSP 1 Score: 643 bits (1658), Expect = 1.91e-232
Identity = 329/335 (98.21%), Postives = 330/335 (98.51%), Query Frame = 0

Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60
           MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60

Query: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120
           IIPARNLKAAEEAKARIVSES+CFD SRITVMALDLSSV SVVNFVSNFESLNLPLNLLI
Sbjct: 61  IIPARNLKAAEEAKARIVSESNCFDRSRITVMALDLSSVKSVVNFVSNFESLNLPLNLLI 120

Query: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180
           NNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH
Sbjct: 121 NNAGRFCYEHALSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180

Query: 181 SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240
           SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP
Sbjct: 181 SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240

Query: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 300
           GVVKTNLNREREGFVTDLIFYMASKSRLLK IPEGAATSCYVATNRNVENVNG YFADCN
Sbjct: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKIIPEGAATSCYVATNRNVENVNGKYFADCN 300

Query: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335
           DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP
Sbjct: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335

BLAST of Csor.00g189640 vs. ExPASy TrEMBL
Match: A0A6J1HL83 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111465182 PE=4 SV=1)

HSP 1 Score: 637 bits (1642), Expect = 5.06e-230
Identity = 328/335 (97.91%), Postives = 329/335 (98.21%), Query Frame = 0

Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60
           MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60

Query: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120
           IIPARNLKAAEEAKARIVSES+CFD SRITVMALDLSSV SVVNFVSNFESLNLPLNLLI
Sbjct: 61  IIPARNLKAAEEAKARIVSESNCFDRSRITVMALDLSSVKSVVNFVSNFESLNLPLNLLI 120

Query: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180
           NNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH
Sbjct: 121 NNAGRFCYEHALSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180

Query: 181 SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240
           SWFSGDIFEYLGQISQKN RVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP
Sbjct: 181 SWFSGDIFEYLGQISQKN-RVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240

Query: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 300
           GVVKTNLNREREGFVTDLIFYMASKSRLLK IPEGAATSCYVATNRNVENVNG YFADCN
Sbjct: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKIIPEGAATSCYVATNRNVENVNGKYFADCN 300

Query: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335
           DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP
Sbjct: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 334

BLAST of Csor.00g189640 vs. ExPASy TrEMBL
Match: A0A5A7UCR1 (Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold344G00880 PE=4 SV=1)

HSP 1 Score: 565 bits (1455), Expect = 2.04e-201
Identity = 288/341 (84.46%), Postives = 313/341 (91.79%), Query Frame = 0

Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC--MNSITAIVTGATSGIGEETARVLAKRGV 60
           MLEM KYLVGSAGASGYGS STAEEV++S   +NSITAIVTGATSGIGEETARVLAKRG 
Sbjct: 1   MLEMLKYLVGSAGASGYGSNSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA 60

Query: 61  RIIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNL 120
           RI+IPARNLKAAEEAKARIVSE +CFD+SRITVM LDLSS+NSV+NFVS FE+LNLPLNL
Sbjct: 61  RIVIPARNLKAAEEAKARIVSELECFDYSRITVMVLDLSSLNSVMNFVSEFEALNLPLNL 120

Query: 121 LINNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSN 180
           LINNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLN+MIETAKSTG+QGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHATSEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLGQISQK----NSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVT 240
           IHSWFSGDIFEYL QIS+     NSR YD+TRAY+FSKLAN+LHTVQLAH+LQEMKANVT
Sbjct: 181 IHSWFSGDIFEYLSQISRNYNNNNSREYDATRAYSFSKLANLLHTVQLAHRLQEMKANVT 240

Query: 241 VNCVHPGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGN 300
           VNCVHPGVV+TNLNR+REGF+ DLIF+MASKS+LLKTIP+ AATSCYVAT+R VENVNG 
Sbjct: 241 VNCVHPGVVRTNLNRDREGFLKDLIFFMASKSKLLKTIPQSAATSCYVATHRKVENVNGK 300

Query: 301 YFADCNDQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335
           YF DCN+QGSCE  TKSAHLAE LWSASE I+S LTG PQP
Sbjct: 301 YFEDCNEQGSCESNTKSAHLAERLWSASEIIISNLTGNPQP 341

BLAST of Csor.00g189640 vs. TAIR 10
Match: AT4G24050.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 417.9 bits (1073), Expect = 7.5e-117
Identity = 221/328 (67.38%), Postives = 268/328 (81.71%), Query Frame = 0

Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC-MNSITAIVTGATSGIGEETARVLAKRGVR 60
           M+E  KYL+G+AGASG+GSKSTAEEV+E+C + SITA++TGATSGIG ETARVLAKRG R
Sbjct: 1   MIETGKYLLGAAGASGFGSKSTAEEVTENCDLRSITAVITGATSGIGAETARVLAKRGAR 60

Query: 61  IIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLL 120
           +I PARN+KAAEEAK RIVSE   F  + I VM LDLSS+ SV NFV++FESL+LPLNLL
Sbjct: 61  LIFPARNVKAAEEAKERIVSE---FPETEIVVMKLDLSSIASVRNFVADFESLDLPLNLL 120

Query: 121 INNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNI 180
           INNAG+  +EHA SEDGIEMTFATNYLGHFLLT LLLN+MI+TA+ TGVQGRIVNVTS I
Sbjct: 121 INNAGKLAHEHAISEDGIEMTFATNYLGHFLLTNLLLNKMIQTAEETGVQGRIVNVTSGI 180

Query: 181 HSWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVH 240
           H WFSGD+ EYL  ISQ   + +D+TRAYA SKLANVLHT +L+ +LQ++ ANVTVNCVH
Sbjct: 181 HGWFSGDLIEYLRLISQPKCQ-FDATRAYALSKLANVLHTKELSSRLQKIGANVTVNCVH 240

Query: 241 PGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADC 300
           PGVV+T L R+REG +TDL+F++ASK  L+KT+P+ AAT+CYVATN  + NV+G YF DC
Sbjct: 241 PGVVRTRLTRDREGLLTDLVFFLASK--LVKTVPQAAATTCYVATNPRLVNVSGKYFTDC 300

Query: 301 NDQGSCECKTKSAHLAEGLWSASETIVS 328
           N+       T S+  A  LW+ASE +V+
Sbjct: 301 NETTPSGLGTNSSE-ATKLWAASEILVT 321

BLAST of Csor.00g189640 vs. TAIR 10
Match: AT1G64590.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 401.0 bits (1029), Expect = 9.5e-112
Identity = 211/333 (63.36%), Postives = 263/333 (78.98%), Query Frame = 0

Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVS-ESCMNSITAIVTGATSGIGEETARVLAKRGVR 60
           M+E  K+L+GS G SG+GS+STA+ V+  S + S+TAI+TGATSGIG ETARVLAKRG R
Sbjct: 1   MIETVKHLIGSGGPSGFGSRSTADHVTCNSDLRSLTAIITGATSGIGAETARVLAKRGAR 60

Query: 61  IIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLL 120
           +++PAR++K AEE KARI+SE   F  + I VM LDLSS+ SV  FV +FESLNLPLN+L
Sbjct: 61  LVLPARSVKTAEETKARILSE---FPDAEIIVMHLDLSSLTSVRRFVDDFESLNLPLNIL 120

Query: 121 INNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNI 180
           INNAG++ ++HA SEDG+EMTFATNYLGHFLLTKLLL +MIETA  TGVQGRIVNVTS +
Sbjct: 121 INNAGKYAHKHALSEDGVEMTFATNYLGHFLLTKLLLKKMIETAAQTGVQGRIVNVTSVV 180

Query: 181 HSWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVH 240
           HSWFSGD+ +YL  IS +N+R YD+TRAYA SKLANVLHTV+L+  L +M ANVT NCVH
Sbjct: 181 HSWFSGDMLQYLADIS-RNNRNYDATRAYALSKLANVLHTVELSRLLHKMDANVTANCVH 240

Query: 241 PGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADC 300
           PG+VKT L R+REG VTDL+F++ SK  LLK++P+ AAT+CYVAT+  + NV G YF+DC
Sbjct: 241 PGIVKTRLTRDREGVVTDLVFFLTSK--LLKSVPQAAATTCYVATSPRLRNVCGKYFSDC 300

Query: 301 ND-----QGSCECKTKSAHLAEGLWSASETIVS 328
           N+      GSC  K      A+ LW+AS+ +VS
Sbjct: 301 NEARSSKSGSCNLK------AQRLWTASDLLVS 321

BLAST of Csor.00g189640 vs. TAIR 10
Match: AT5G50130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 287.0 bits (733), Expect = 2.0e-77
Identity = 157/324 (48.46%), Postives = 226/324 (69.75%), Query Frame = 0

Query: 6   KYLVGSAGASGYGSKSTAEEVSESCM---NSITAIVTGATSGIGEETARVLAKRGVRIII 65
           +YL G AG +G+GS+STAE+V++      + +TAI+TG TSGIG ETARVLAKRGVR+++
Sbjct: 6   RYLAGIAGPNGFGSRSTAEQVTQHSFFPCSHLTAIITGGTSGIGAETARVLAKRGVRVVM 65

Query: 66  PARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLINN 125
             R++K AE  K RI+ E+   D   I +  +DLSS++SV  F S F S +LPLN+LINN
Sbjct: 66  AVRDMKKAEMVKERIIRENPEAD---IILFEIDLSSLSSVARFCSQFLSQDLPLNILINN 125

Query: 126 AGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIHSW 185
           AG F     FSE+ IE+TFATN+LGH+LLT++L+ +MI+TA+ +G++GRI+N++S IH+W
Sbjct: 126 AGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVIHNW 185

Query: 186 FSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPGV 245
              D F +  ++    SR Y+ TRAYA SKLA +LH   L+ +L++  ANVT+N VHPG+
Sbjct: 186 VKPDCFSF-PKLLHPISR-YNGTRAYAQSKLATILHAKALSKQLKDRNANVTINAVHPGI 245

Query: 246 VKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCNDQ 305
           VKT + R  +G  TD +F +ASK  LLK+I +GAAT+CYVA +   + ++G YFADCN+ 
Sbjct: 246 VKTGIIRAHKGLFTDSLFLIASK--LLKSISQGAATTCYVALSNETKGLSGKYFADCNET 305

Query: 306 GSCECKTKSAHLAEGLWSASETIV 327
            +C       ++A  L + S  ++
Sbjct: 306 -NCSDLANDEYVALKLCTQSRALI 321

BLAST of Csor.00g189640 vs. TAIR 10
Match: AT5G02540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 264.2 bits (674), Expect = 1.4e-70
Identity = 152/332 (45.78%), Postives = 218/332 (65.66%), Query Frame = 0

Query: 4   MFKYLVGSAGASGYGSKSTAEEVSESC-MNSITAIVTGATSGIGEETARVLAKRGVRIII 63
           ++  + G  G SG+GS STAEEV++     ++TAI+TG T GIG ETARVL+KRG  ++I
Sbjct: 3   LYSLITGRRGPSGFGSASTAEEVTQGIDATNLTAIITGGTGGIGMETARVLSKRGAHVVI 62

Query: 64  PARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLINN 123
            ARN+ AAE AK  I+ ++    ++R+T++ LDLSS+ S+  FV  F +L+LPLNLLINN
Sbjct: 63  GARNMGAAENAKTEILRQN---ANARVTLLQLDLSSIKSIKAFVREFHALHLPLNLLINN 122

Query: 124 AGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTS--NIH 183
           AG     +  SEDGIE+ FATN++GHFLLT LLL+ M  TAK++GV+GRI+NV+S  +I+
Sbjct: 123 AGVMFCPYQLSEDGIELQFATNHIGHFLLTNLLLDTMKNTAKTSGVEGRILNVSSVAHIY 182

Query: 184 SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 243
           ++  G  F+ +  I       Y   RAY  SKLAN+LH  +L+ +LQE   N+T N VHP
Sbjct: 183 TYQEGIQFDSINDICS-----YSDKRAYGQSKLANILHANELSRQLQEEGVNITANSVHP 242

Query: 244 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 303
           G++ TNL +     +  L F+      L K IP+GAAT+CYVA + +V+ V G YFADCN
Sbjct: 243 GLILTNLFQHTALLMRFLKFF---SFYLWKNIPQGAATTCYVALHPSVKGVTGKYFADCN 302

Query: 304 DQGSCECKTKSAHLAEGLWSASETIVSTLTGK 333
           +    +   +   LA+ LW  S  ++++++ K
Sbjct: 303 EVTPSKL-ARDETLAQKLWDFSVKLINSVSKK 322

BLAST of Csor.00g189640 vs. TAIR 10
Match: AT5G50130.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 251.1 bits (640), Expect = 1.2e-66
Identity = 138/272 (50.74%), Postives = 195/272 (71.69%), Query Frame = 0

Query: 6   KYLVGSAGASGYGSKSTAEEVSESCM---NSITAIVTGATSGIGEETARVLAKRGVRIII 65
           +YL G AG +G+GS+STAE+V++      + +TAI+TG TSGIG ETARVLAKRGVR+++
Sbjct: 6   RYLAGIAGPNGFGSRSTAEQVTQHSFFPCSHLTAIITGGTSGIGAETARVLAKRGVRVVM 65

Query: 66  PARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLINN 125
             R++K AE  K RI+ E+   D   I +  +DLSS++SV  F S F S +LPLN+LINN
Sbjct: 66  AVRDMKKAEMVKERIIRENPEAD---IILFEIDLSSLSSVARFCSQFLSQDLPLNILINN 125

Query: 126 AGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIHSW 185
           AG F     FSE+ IE+TFATN+LGH+LLT++L+ +MI+TA+ +G++GRI+N++S IH+W
Sbjct: 126 AGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVIHNW 185

Query: 186 FSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPGV 245
              D F +  ++    SR Y+ TRAYA SKLA +LH   L+ +L++  ANVT+N VHPG+
Sbjct: 186 VKPDCFSF-PKLLHPISR-YNGTRAYAQSKLATILHAKALSKQLKDRNANVTINAVHPGI 245

Query: 246 VKTNLNREREGFVTDLIFYMASKSRLLKTIPE 275
           VKT + R  +G  TD +F +ASK  LLK+I +
Sbjct: 246 VKTGIIRAHKGLFTDSLFLIASK--LLKSISQ 270

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A078IS663.5e-6645.02Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=T... [more]
A0A078ISJ65.9e-6645.02Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=T... [more]
A2RVM09.8e-6144.38Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
Q6RVV44.7e-5543.17Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC3... [more]
Q9HBH58.9e-3837.94Retinol dehydrogenase 14 OS=Homo sapiens OX=9606 GN=RDH14 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022923250.19.05e-238100.00short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita mosch... [more]
XP_023552711.11.06e-23699.40short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita pepo ... [more]
XP_022923251.12.40e-23599.70short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita mosch... [more]
XP_023552712.12.80e-23499.10short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita pepo ... [more]
XP_022965265.13.95e-23298.21short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita maxim... [more]
Match NameE-valueIdentityDescription
A0A6J1EB904.38e-238100.00short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita mos... [more]
A0A6J1E6B31.16e-23599.70short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita mos... [more]
A0A6J1HN851.91e-23298.21short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita max... [more]
A0A6J1HL835.06e-23097.91short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita max... [more]
A0A5A7UCR12.04e-20184.46Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
Match NameE-valueIdentityDescription
AT4G24050.17.5e-11767.38NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G64590.19.5e-11263.36NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G50130.12.0e-7748.46NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G02540.11.4e-7045.78NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G50130.21.2e-6650.74NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 114..125
score: 42.55
coord: 164..180
score: 27.08
coord: 208..227
score: 30.23
coord: 35..52
score: 46.16
coord: 231..248
score: 36.77
IPR002347Short-chain dehydrogenase/reductase SDRPFAMPF00106adh_shortcoord: 35..178
e-value: 4.6E-25
score: 88.2
NoneNo IPR availableGENE3D3.40.50.720coord: 28..329
e-value: 8.6E-69
score: 233.8
NoneNo IPR availablePANTHERPTHR24320RETINOL DEHYDROGENASEcoord: 4..329
NoneNo IPR availablePANTHERPTHR24320:SF114OS06G0129100 PROTEINcoord: 4..329
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 35..300

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g189640.m01Csor.00g189640.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity