Csor.00g188270 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g188270
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionbeta-amyrin 11-oxidase-like isoform X1
LocationCsor_Chr16: 8001559 .. 8008520 (-)
RNA-Seq ExpressionCsor.00g188270
SyntenyCsor.00g188270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTAACAATGGGGGTGAAGAACTGGTTGGTGTTGATTGTTGCAGCCTTTGTGTTTGTTTTGGGGATTTTGAAGAGAGTAAATGGTTGGTATTATGCGGCCAAGCTTGGTAAAACATGGTCTATGCTTCCTCCTGGTGATATGGGTTGGCCTCTTCTTGGTTCTACCATATCATACATCAAGAGTTTCATCTCTGGTCAGCCAAGTAATTTCATTGCCAACTTTTACATAAGGTCATTTCTTCATTCATTTCATTCAAGTTTATGATTAATATGTTCAAATAATATGTTTCTTTCCTCATTCTACTTAGAATGTTGCATCAAAATTGACATCTGAAACGGGTGTAATCGTATTGTTGAAGTCTTATATTGGTTTATTAAGGGGAAGATCATGGGTATATAAGTAGAAAACACTATCTATCAGACCTTCTGGAAAATCAAAAGCAAAGTCTGAACAATATCATACCGTTGTGTAGGTTCGTTGTACCGTTGTGTAGGTTCGTTGTCTCTAACAAATGGTATCAAAGTCATGCCCTTAACAGACATATCAATAGAATTTTCAGGTGTCGAACAAATTAAAGATTTATGGTAGAGCTCGATTAAACTAGCAAAAAAAAAAAAAAAAAAAATTGTTGTGCGGGATAGTGCTAGACGAATGGATGACTATTTAAAAAGAGCCTCAATGATACTTTGTTCGAGAGGAGGATGGTTGGAGTGGTTAATTGAGACCCTTACGTGGAAACCAAAATCAAAACCATGAAAATGTATGTTAAAGTGGACAATTTCATACCATTTATTGAGGTTCGTTGTTACTAATAGGTGTTTGGATCCTAATTTTTATTCCAAGTCACTGTCACACGTCGTTTTCAAACCTTCTAATGAATTACTACTCCAAACTTCAAGAGTATATTTCGTATAATTTAGCTTTCATTTGACATATAATTGTTACTTTAGAAATTACTCTCAGAAATGAAGAAGCGATGCATCAAAGTCTTAATGAATGTTAGCTAATTGTCGATCAAAATATCAGAGTTCGATCATAGCTCAAACGATAAGAATCAAGACACTATTTTGATATCTCTCATCGAACTTCTTTTTTTTTTTTTTTTACAGATATGGTAAAGGTGATATGTACAAAACACACATACTTGGTAGAGCAACTATAATTGTTTGTTCATCTGAACTTTGTCGTCAAGTCTTAGGCGATGAAGACAACTTCAAACCTAGTTTACCCAAGAACATCAATATGCTAACCGGTAGAAAGTCACTGGTACAAGTGTTCAAGGCGGAGCACAAGCGGCTTCGGCGGTTGACGATGGCGCCGATCAGCGGCCACGCCGCGCTGGAGATGTACATCAATCATATTGAACACACAGTGATGAGTGGGTTAGAGGAATGGGCAAGCATGAAGAACCCAATTGAGGTATTGTCAGAGATAAAGAAGCTGACCTTCAAAATTATTTGGAACATTTTCATGGGTTCTTCTTCTTTAGAGTCTTCCATTGGAGAAATGGAGGGTTTGTTTTCTGAAGTTGCTTTAGGGTTCATATCATTGCCCATCAACTTTCCTGGCTTCAACCTCCATAAAGCACTCAAGGTAAGCCCCTTCCTTCTTTTCTTTCCTTTTTACCTTTGTTTCTTGTCAATAATTCATGTGGGATTTGAATAAGTAGTTGTATTTGATCATCGAAACGGCTAAAAAGACTAAATTAACGGTAACTTATGTTGAGAATTGTTAGGAGAGAAGTCCAAGTAAAGAATACTTCTGCGTTAGTACAGGGGCATTTTGAAAAAACCTAAAGTAAAGTCACGAGAATTTATTCTCAAAGTGCATAATATCATATTATCGTATCAGAGTCGTGTCCTTGATTTAGTCATGTCAATAGAATCCTAAAAAAAAAAGAAATTGTGAGTGTGAGAACCCACATTGATTGGAGAGCAAAATGAGTGCCAGTGAGGATGTTGGGCTCCGAAGGGAGGTGGATTGTGAGATCCCACATTGGTTAGAGAAGAGAACGAAACATTTTTTAAAAGAGTTAAGAAAACTCTCCCTAACAAACGCGTTTTAAAAACCTTGAGGGAAAACCCGAAAGGAGCAGTGGGCTTGAGTTGTTATAGTGAGCCTCAAAGGTGTAGTCAAAAGTGACTCAAGTGTTGAACAAAATGTCTATTTTATTCAAGGGCTTCGGAGGAGTCGAGCCTCGAAGGTTGTTCGAGGGCTACATAGCCTCAGAGGAGGCTCTATGGTGTGGGAACCCACATCCGTTGGAGAGGAGAATGAAACATTGTTTATAAGGGTGTGAGAACTTCTCCGTAGTATATGTGTTTTAAAAACCTAAAAGTTCGAAAGGAAAAGTCTAAAGAGGACAATATCTGCTAGCGGTGGACTTGAGTTATTACATATGGTGTATTTTGTCGGGAGAGGAGTCCAAATAAAGAATACATCTCCATTGGTATGAACTCTATTGAAAAAACCGAAAATAAAATTATAAGAGGGTCGTTATTAATAACAACTTATCCACGAACGTAGCAATAATTTAACATAGTATTATACAAAGTTCTATGTTTGAATCCTTATAATGTCGTGGTCATCTCTAGTTCATTTTAGATTAAAATATACATTTCTTTACTAATACTTAATTGTATTTGTCACTATGTTACCGCTAAGAGGGATTTAAACTATAATATTTGAAACTAATTCAACATTGTTAGGTTGGACTTATTGTATTCTTTAAATTATATTTCATATCTTTCCTCTCTCATTTGATCAATGCAAAATAATACTTTTTTTATAGTAATTAAATATATTTTGCAGTTAATCCCTTGATTATGCCAAATTCAAACCTTGGAAATTTCAAAGGTTTGGCCATTTAATTAAATAACCTAGTGTTAAAATAAATCGAAAATCATTCCTAGTTTTTAATTATTGTTGTTTAAGGTAACCTGCAATTAAAGTAGGTGAGAGTCGGAGTAGAAGGGCTTTAATTAAGTTACCCACACAATTAAACATTTCCACTCGGGTTCGCTTAGTCAAAGATAAGACTTAGTACATTGCCCAAAAAAATAATAATAATAAAATACTAGTGGAGCAAAATTCAAAGTTGAGGATTGTTGAGAAAATAGTCAAGTGAAGAATACAGCTCGATTGGTACGAGACTTTTTCGGAATTTTAAAAGCAAGACCTTTGGGGAATATCAAAAGCAAAGTCATAAGAGTTTATGCTCAAAATGGACAATATCATATTATTGTAGAGGATCATGATTACTAATCGTATCAAAGTCATGCCCTTAACTTAGTCATATCAATAGAATTCTCAAGTGTCGAACAAAAAACTTGTGAGTCTCGAATGTATAGTCAAAAGTGACTCAAATGTTGAACAAATGGTGTACTTTATTTGAGGGCTCCAGAGAAAGAGTCGAACCTCGATTAAGGGGAGGTTGTTCGAGGGCCCCATAGGCCTCAAAGGAGACTCTATGGTATACTTTGTTCAATGAGAGAATTGCTAAGGATTGTTGGGAGATGAGTACAAGTAAAGAATACATCTCCATTGATATTAACCCTTTTGGGGAAACCAAAAGTAAAGACATGAGAGCTTATGTTCAAAGTAGACAATATCATACCATTATTGTGAGATCCCACATCGGTTCGGAAGGAGAACGAAACAAATTTTTTAAGAGAAGCTTGAAAGGAAAAATCTCAAAAAAGACAATATCTACTAGTGATAGGCTTTGTCATTACAATTATAAAGGGTCGTGATTACTATCAACTTATCAACAAGTTTACACAGTAATAATTTAACATGGTATCAAATACAAGGTCGTGTTTGAATTCTTAGAATGTCATCGTTCTCTCTAGTTCATTGTAGATTAAAAGATGTGTTCCTTTACTAATACTTGAGAATGACATATACTTATTGTATTTACGTCACTATCTTACAGCTAAGAGATTTAAACTATAATATTTGAAACTAATTCATCATTGTTAGGTTGGACTTATTATATGCTTTCTCTATCTCATTTGATCAATGCAAAATAATATATATTTTTTTATTGTAATTAAATATATTTTGTAGTTAATATCTTGATTATGCCAAATTTAAACCTCTCACAAAGTCCAAACTAGGTTAAATTAATAAAAATACTCTCGAATTTTGCACTTTTATCTAAACAAAGTTTCAAAAAGTTCAAAAACACGGGTTAGTATTCTGTTTCAAAAATATCTTTTTTCTGGATATTTTTAAACATGTTACCAATTCGAGGTATTAATGAAATTTTTAAAAAATCAAGGGTATTTTTTAACAGATTAAAAGTACAAAGTATTTTTGAAACAAAGTACTAATAGTTTCCATCTAACGGCAAGGGTATTTTTTTTAGACTCATTCAAAAGTTTTTAGGGTATATTAAAAGTTTTAGGGTATTTTTGAAATAAAAGTATACCGCTTAGAGGTACTTTTATTAATTTAGCATCAAAACTATTTCATATCAACACCATACTCACTCCAAGTCTTAATTTTCATTAAGGCCACTATTGTAACTTTTGAAAGAACGGGAGTATGTTTAAAATAAATAGCAAACTTCAGTGGTATTTTTGTAATTTACTACCTTAAATTTATCCAAACCCATCAAATAAACCGGTAGACGAGTAAATACGCTAATTAAATAGATAAATTTTCATGTAAATTATAGCTTGAATTATTGTTTTTTTTTTTTTTTTTTTTTTTCCTGTTAACATATTTAAACATAGGCTAGAAAGAAGTTGCTCAACATACTTCAATCCATCATCAACCAAAAGAGATTGGTGAGGGAAAGGCAAGGAACAAGTTGGGAAGCTAAAGACATGATGGATTTGCTTATGGAAGTGAAAGATGAAGATGGTGAAGAGTTGGATGATGAAACCATTAGAGATTTGATATTTGGTAAGCTATTTGCAGGCCATGAAACTTCAGCTTATACAGCAATGTGGGCGGTTTTGTTTCTTACAAAACATCCACATATTTTCCAAAGAGCCAAGGTTAGTGATTATTTCTTTCTTAACTTTGCTACATTTCATGTTCTTTTCAACTATATAATATTTGGGATTTGTAGTATTTATTATATGATCTATTATATTATAAAATCTATGTGTTTTAGGAAGAACAAGAACGTATCGTTACCCAAAGACCCACGACTCAAAAGGGTATAATTCTCTCTGAGGTTAAACAGATGAAATTTCTTTATCAGGTATGATTTTAATATTTATTTTCCTTGAGATTGAAATTACTCATTTATATTGTGAGATCCCACCTCGGTTACAGAGAGGAATGAAACATTCCTTAAAAGGGTGTGGAAACCTCTCCCTAGTAGACACGTTTTAAAACAATGAGGTTGATGGCAATACATAACGGGCCAAAGCGAATAATATCTACTAGCGGTGGACTTGAGTTGTGTTACAAATGGTATCAGAATCAAACACCTTGTGCTAGTGAGGACATTGGCTCCCAAGAGGAGTGGATTGTGAGATCCACATCGATTGAAAAGGGGAACAAAGCATTCCTCACGAGGGTGTGGAAACCTCTCCTGTAGATCGTGAGACTCCATAACGTTGGAGAAGGAAACGAAACATTTTTTATAAGAGTGTGGAGACCTCTCCCTAGTAGACGTGTTTTAAAACTGTGAGACTGACGACGATACATAACAGGCCAAAGCGAACATTAGGAAATCAAAATTAAGAATACATTTTTTGATATTTCAGGTTATAGATGAGACGCTACGTGTTGGTGGTGTGACATTAATGCTTTTTCGTGAAGCAGTAGTTGATGTCGAAATTAATGGTAAGTGATTAAATTTCTATACATATATGTATGTCTTCTAACACATGAATTATGATTCCTTGTACTTTATTTAGGTAAGATTATACCAAAGGGATGGAAAGTCATACCTTGGTTTGGGGAGCTTTATATGGATGAAAAGCTATTTCCTTCACCACAAGAGTTCAACCCTTCAAGATGGGATGTATGTAACTTATTTATGGGCAATATATTAGATAATCATTATTCTAAAGGGTATTCAAAAGATTCCACGTTTTCATATCCTTGTAATGATGCTTCGTTCTTCGTCTTCAAATTTTTATAAGGGATGCTTCGTTCCTCGTTTTCACAATCTTATAAGAGATGTTTCGTGCCCCATTTCCACACCCTTATAAGAGATTCTTTGTGCCCCGTTTCTACACCCTTATAAGAAATTCTTCGTGCCCTGTTTTCACACCTTTACAAAGGATGCTTATTGTCCCGTTTTCATACCCTTATAAGGAATGCTTCCTGCTTAGTTCTCCGTTTTGATCCTCGTTTTCACACCTTTAGAAGGAAGGCTTTGTTTCCTTCTCTAACCAATGTGGGATCTCAAAGAGGTTTTCACACCTTTATACGTAATTCTCGTTTCCACACCTTTAGAACGAACGTTTCATTTCATTCTCTAACCAATATGGGATCTCAAAGAGGTTTTCACACCTTTATAAGGAATCCTTGTTTCCACACCTTTAGAATAAATGTTTCGTTTCCTTCTCTAACCATTGTAGGATCTCAGAGAGGTTTTCACACCTTCTAAACGACGTGCATTTTGTTTTCTCTTTTGATCTAATTAATGGCATATTGAATGGAATTTTTCTTGTTGTTTCATAAAGAATTATGTAGCCACGCCAGGAGCTTTCATCCCTTTTGGATCAGGAAGCAGGTATTGCCCCGGAAGTGATCTTGCCAAGCTCGAAATCTCAATTTTTCTTCATTATTTTCTCCTCAATTACAAGTAAGTTTACTCTTAACTTCTCATTAACTTTAACACAAAATTTAAACAAAAAGCTTTTCTTAATTACTACACAATCATTTTGTAATAATGCAGGATTGAACAGCTTAATCCAAAATGTCCGATGACTTACATGCCATTTCCTCATCCCAAAGACAAATGCTTGGCAAGAGTGCATAAAGTTTAA

mRNA sequence

ATGTTAACAATGGGGGTGAAGAACTGGTTGGTGTTGATTGTTGCAGCCTTTGTGTTTGTTTTGGGGATTTTGAAGAGAGTAAATGGTTGGTATTATGCGGCCAAGCTTGGTAAAACATGGTCTATGCTTCCTCCTGGTGATATGGGTTGGCCTCTTCTTGGTTCTACCATATCATACATCAAGAGTTTCATCTCTGGTCAGCCAAGTAATTTCATTGCCAACTTTTACATAAGATATGGTAAAGGTGATATGTACAAAACACACATACTTGGTAGAGCAACTATAATTGTTTGTTCATCTGAACTTTGTCGTCAAGTCTTAGGCGATGAAGACAACTTCAAACCTAGTTTACCCAAGAACATCAATATGCTAACCGGTAGAAAGTCACTGGTACAAGTGTTCAAGGCGGAGCACAAGCGGCTTCGGCGGTTGACGATGGCGCCGATCAGCGGCCACGCCGCGCTGGAGATGTACATCAATCATATTGAACACACAGTGATGAGTGGGTTAGAGGAATGGGCAAGCATGAAGAACCCAATTGAGGTATTGTCAGAGATAAAGAAGCTGACCTTCAAAATTATTTGGAACATTTTCATGGGTTCTTCTTCTTTAGAGTCTTCCATTGGAGAAATGGAGGGTTTGTTTTCTGAAGTTGCTTTAGGGTTCATATCATTGCCCATCAACTTTCCTGGCTTCAACCTCCATAAAGCACTCAAGGCTAGAAAGAAGTTGCTCAACATACTTCAATCCATCATCAACCAAAAGAGATTGGTGAGGGAAAGGCAAGGAACAAGTTGGGAAGCTAAAGACATGATGGATTTGCTTATGGAAGTGAAAGATGAAGATGGTGAAGAGTTGGATGATGAAACCATTAGAGATTTGATATTTGGTAAGCTATTTGCAGGCCATGAAACTTCAGCTTATACAGCAATGTGGGCGGTTTTGTTTCTTACAAAACATCCACATATTTTCCAAAGAGCCAAGGAAGAACAAGAACGTATCGTTACCCAAAGACCCACGACTCAAAAGGGTATAATTCTCTCTGAGGTTAAACAGATGAAATTTCTTTATCAGGTTATAGATGAGACGCTACGTGTTGGTGGTGTGACATTAATGCTTTTTCGTGAAGCAGTAGTTGATGTCGAAATTAATGGTAAGATTATACCAAAGGGATGGAAAGTCATACCTTGGTTTGGGGAGCTTTATATGGATGAAAAGCTATTTCCTTCACCACAAGAGTTCAACCCTTCAAGATGGGATAATTATGTAGCCACGCCAGGAGCTTTCATCCCTTTTGGATCAGGAAGCAGGTATTGCCCCGGAAGTGATCTTGCCAAGCTCGAAATCTCAATTTTTCTTCATTATTTTCTCCTCAATTACAAGATTGAACAGCTTAATCCAAAATGTCCGATGACTTACATGCCATTTCCTCATCCCAAAGACAAATGCTTGGCAAGAGTGCATAAAGTTTAA

Coding sequence (CDS)

ATGTTAACAATGGGGGTGAAGAACTGGTTGGTGTTGATTGTTGCAGCCTTTGTGTTTGTTTTGGGGATTTTGAAGAGAGTAAATGGTTGGTATTATGCGGCCAAGCTTGGTAAAACATGGTCTATGCTTCCTCCTGGTGATATGGGTTGGCCTCTTCTTGGTTCTACCATATCATACATCAAGAGTTTCATCTCTGGTCAGCCAAGTAATTTCATTGCCAACTTTTACATAAGATATGGTAAAGGTGATATGTACAAAACACACATACTTGGTAGAGCAACTATAATTGTTTGTTCATCTGAACTTTGTCGTCAAGTCTTAGGCGATGAAGACAACTTCAAACCTAGTTTACCCAAGAACATCAATATGCTAACCGGTAGAAAGTCACTGGTACAAGTGTTCAAGGCGGAGCACAAGCGGCTTCGGCGGTTGACGATGGCGCCGATCAGCGGCCACGCCGCGCTGGAGATGTACATCAATCATATTGAACACACAGTGATGAGTGGGTTAGAGGAATGGGCAAGCATGAAGAACCCAATTGAGGTATTGTCAGAGATAAAGAAGCTGACCTTCAAAATTATTTGGAACATTTTCATGGGTTCTTCTTCTTTAGAGTCTTCCATTGGAGAAATGGAGGGTTTGTTTTCTGAAGTTGCTTTAGGGTTCATATCATTGCCCATCAACTTTCCTGGCTTCAACCTCCATAAAGCACTCAAGGCTAGAAAGAAGTTGCTCAACATACTTCAATCCATCATCAACCAAAAGAGATTGGTGAGGGAAAGGCAAGGAACAAGTTGGGAAGCTAAAGACATGATGGATTTGCTTATGGAAGTGAAAGATGAAGATGGTGAAGAGTTGGATGATGAAACCATTAGAGATTTGATATTTGGTAAGCTATTTGCAGGCCATGAAACTTCAGCTTATACAGCAATGTGGGCGGTTTTGTTTCTTACAAAACATCCACATATTTTCCAAAGAGCCAAGGAAGAACAAGAACGTATCGTTACCCAAAGACCCACGACTCAAAAGGGTATAATTCTCTCTGAGGTTAAACAGATGAAATTTCTTTATCAGGTTATAGATGAGACGCTACGTGTTGGTGGTGTGACATTAATGCTTTTTCGTGAAGCAGTAGTTGATGTCGAAATTAATGGTAAGATTATACCAAAGGGATGGAAAGTCATACCTTGGTTTGGGGAGCTTTATATGGATGAAAAGCTATTTCCTTCACCACAAGAGTTCAACCCTTCAAGATGGGATAATTATGTAGCCACGCCAGGAGCTTTCATCCCTTTTGGATCAGGAAGCAGGTATTGCCCCGGAAGTGATCTTGCCAAGCTCGAAATCTCAATTTTTCTTCATTATTTTCTCCTCAATTACAAGATTGAACAGCTTAATCCAAAATGTCCGATGACTTACATGCCATTTCCTCATCCCAAAGACAAATGCTTGGCAAGAGTGCATAAAGTTTAA

Protein sequence

MLTMGVKNWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWSMLPPGDMGWPLLGSTISYIKSFISGQPSNFIANFYIRYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKNINMLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIEVLSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARKKLLNILQSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLFAGHETSAYTAMWAVLFLTKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVIDETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWDNYVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPKDKCLARVHKV
Homology
BLAST of Csor.00g188270 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 7.1e-145
Identity = 243/481 (50.52%), Postives = 347/481 (72.14%), Query Frame = 0

Query: 13  IVAAFVFVLGILKRVNGWYYAAKLGKTWSMLPPGDMGWPLLGSTISYIKSFISGQPSNFI 72
           ++  ++F    ++ +NGWYY  KL +    LPPGDMGWPL+G  +S+IK F SG P +FI
Sbjct: 13  LLVCYIFGSKFVRNLNGWYYDVKLRRKEHPLPPGDMGWPLIGDLLSFIKDFSSGHPDSFI 72

Query: 73  ANFYIRYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKNINMLTGRKSLVQ 132
            N  ++YG+  +YKTH+ G  +IIVC  ++CR+VL D+ NFK   PK+I  L   + ++ 
Sbjct: 73  NNLVLKYGRSGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYPKSIKELARCRPMID 132

Query: 133 VFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIEVLSEIKKLTFK 192
           V  AEH+  RRL  +PI GH AL MY+  +E  V++ LEE +SMK+P+E+L E+KK++FK
Sbjct: 133 VSNAEHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSSMKHPVELLKEMKKVSFK 192

Query: 193 IIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARKKLLNILQSII 252
            I ++FMGSS+ +  I ++   F+++  G  S+PIN PGF  HKAL+ARKKL  I+Q ++
Sbjct: 193 AIVHVFMGSSN-QDIIKKIGSSFTDLYNGMFSIPINVPGFTFHKALEARKKLAKIVQPVV 252

Query: 253 NQKRLVRE---RQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLFAGHETSAYT 312
           +++RL+ E   ++G+  + KD++D+L+EVKDE+G +L+DE I DL+ G LFAGHE++A +
Sbjct: 253 DERRLMIENGPQEGS--QRKDLIDILLEVKDENGRKLEDEDISDLLIGLLFAGHESTATS 312

Query: 313 AMWAVLFLTKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVIDETLRVGGV 372
            MW++ +LT+HPHI ++AKEEQE I   R ++QK + L E+KQM +L QVIDETLR   +
Sbjct: 313 LMWSITYLTQHPHILKKAKEEQEEITRTRFSSQKQLSLKEIKQMVYLSQVIDETLRCANI 372

Query: 373 TLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWDNYVATPGAF 432
               FREA  DV ING IIPKGW+V+ W   ++MD + +P+P+EFNPSRWD+Y A  G F
Sbjct: 373 AFATFREATADVNINGYIIPKGWRVLIWARAIHMDSEYYPNPEEFNPSRWDDYNAKAGTF 432

Query: 433 IPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPKDKCLARVHK 491
           +PFG+GSR CPG+DLAKLEISIFLHYFL NY++E++NP+C +T +P   P D CLA+V K
Sbjct: 433 LPFGAGSRLCPGADLAKLEISIFLHYFLRNYRLERINPECHVTSLPVSKPTDNCLAKVIK 490

BLAST of Csor.00g188270 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 2.0e-131
Identity = 232/486 (47.74%), Postives = 330/486 (67.90%), Query Frame = 0

Query: 3   TMGVKNWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWSMLPPGDMGWPLLGSTISYIKS 62
           T  +  W  L+V     +  ++++VN W Y + LG+    LPPGD+GWP +G+ +S++++
Sbjct: 5   TSWIPVWFPLMVLGCFGLNWLVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRA 64

Query: 63  FISGQPSNFIANFYIRYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKNIN 122
           F +  P +F      RYG   +YK H+ G  +IIV +S+ CR+VL D+D FKP  P +  
Sbjct: 65  FKTSDPDSFTRTLIKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTM 124

Query: 123 MLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIEV 182
            L GRKS V +   EHKRLRRLT AP++GH AL  YI +IE  V++ L++W  M    E 
Sbjct: 125 ELIGRKSFVGISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKM-GEFEF 184

Query: 183 LSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARK 242
           L+ ++KLTF+II  IF+ S S E+ +  +E  ++ +  G  ++ +N PGF  H+ALKARK
Sbjct: 185 LTHLRKLTFRIIMYIFLSSES-ENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARK 244

Query: 243 KLLNILQSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLFAG 302
            L+   QSI+ ++R  R +Q      KDM+D L+ VKDEDG+ LDDE I D++   L AG
Sbjct: 245 TLVAAFQSIVTERRNQR-KQNILSNKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAG 304

Query: 303 HETSAYTAMWAVLFLTKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVIDE 362
           HE+S +T MWA +FL +HP + QRAK EQE I+  RP  QKG+ L E ++M+FL QV+DE
Sbjct: 305 HESSGHTIMWATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDE 364

Query: 363 TLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWDN- 422
           TLRV   +L  FREA  DVE+NG +IPKGWKV+ WF ++++D ++FP P++F+P+RWDN 
Sbjct: 365 TLRVITFSLTAFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNG 424

Query: 423 YVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPKD 482
           +V   GAF+PFG+GS  CPG+DLAKLEISIFLH+FLL Y++++ NP+CP+ Y+P   P D
Sbjct: 425 FVPKAGAFLPFGAGSHLCPGNDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTD 484

Query: 483 KCLARV 488
            CLAR+
Sbjct: 485 NCLARI 487

BLAST of Csor.00g188270 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 465.7 bits (1197), Expect = 6.5e-130
Identity = 234/479 (48.85%), Postives = 324/479 (67.64%), Query Frame = 0

Query: 9   WLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWSMLPPGDMGWPLLGSTISYIKSFISGQP 68
           W  LI+     +  +LKRVN W Y +KLG+    LPPGD+GWP++G+  S++++F +  P
Sbjct: 10  WFPLIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDP 69

Query: 69  SNFIANFYIRYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKNINMLTGRK 128
            +FI ++  RYG+  +YK H+ G   ++V + E CR+VL D+D F    PK+   L GRK
Sbjct: 70  ESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRK 129

Query: 129 SLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIEVLSEIKK 188
           S V +   EHKRLRRLT AP++G  AL +YI  IE TV + LE+W+ M   IE LS ++K
Sbjct: 130 SFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKM-GEIEFLSHLRK 189

Query: 189 LTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARKKLLNIL 248
           LTFK+I  IF+ S S E  +  +E  ++ +  G  ++ IN PGF  H+ALKARKKL+   
Sbjct: 190 LTFKVIMYIFLSSES-EHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAF 249

Query: 249 QSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLFAGHETSAY 308
           QSI+  +R  R +Q  S   KDM+D L++VKDE+G  LDDE I DL+   L AGHE+S +
Sbjct: 250 QSIVTNRRNQR-KQNISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGH 309

Query: 309 TAMWAVLFLTKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVIDETLRVGG 368
             MWA + + +HP I Q+AKEEQERIV +R   QK + L E ++M +L QVIDETLRV  
Sbjct: 310 LTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVIT 369

Query: 369 VTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWDNYVATPGA 428
            +L  FREA  DV+++G IIPKGWKV+ WF  +++D +++P P++F+PSRW+ Y    G 
Sbjct: 370 FSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGT 429

Query: 429 FIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPKDKCLARV 488
           F+PFG GS  CPG+DLAKLEISIFLH+FLL Y++E+ NP CP+ ++P   PKD CLAR+
Sbjct: 430 FLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARI 484

BLAST of Csor.00g188270 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 4.4e-118
Identity = 209/480 (43.54%), Postives = 320/480 (66.67%), Query Frame = 0

Query: 13  IVAAFVFVLGILKRVNGWYYAAKLG-KTWSMLPPGDMGWPLLGSTISYIKSFISGQPSNF 72
           +VA  V V  +++R + W   A LG +  S LPPG+MGWP++GS  +++++F SG P  F
Sbjct: 24  VVAGVVLVDAVVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAFKSGNPDAF 83

Query: 73  IANFYIRYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKNINMLTGRKSLV 132
           IA+F  R+G+  +Y+T +    TI+  + E C+QVL D++ F    PK    L G KS V
Sbjct: 84  IASFIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATVTLIGPKSFV 143

Query: 133 QVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKN-PIEVLSEIKKLT 192
            +   +H+R+R+LT API+G  AL  Y++ I+ TV++ L  W+S ++  +E L+E++++T
Sbjct: 144 NMSYDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEFLTELRRMT 203

Query: 193 FKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARKKLLNILQS 252
           FKII  IFM S + ++++  +E  ++++  G  ++ IN PGF  ++AL+AR+KL+++LQ 
Sbjct: 204 FKIIVQIFM-SGADDATMEALERSYTDLNYGMRAMAINLPGFAYYRALRARRKLVSVLQG 263

Query: 253 IINQKRLVRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLFAGHETSAYTA 312
           +++ +R    +      A DMMD L+E +DE G  L D+ I D++   L AGHE+S +  
Sbjct: 264 VLDGRRAAAAKGFKRSGAMDMMDRLIEAEDERGRRLADDEIVDVLIMYLNAGHESSGHIT 323

Query: 313 MWAVLFLTKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVIDETLRVGGVT 372
           MWA +FL ++P IF RAK EQE I+   P TQ G+ L + K+M FL QV+DETLR   ++
Sbjct: 324 MWATVFLQENPDIFARAKAEQEEIMRSIPATQNGLTLRDFKKMHFLSQVVDETLRCVNIS 383

Query: 373 LMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWDNYVATPGAFI 432
            + FR+A  D+ +NG +IPKGWKV  W+  ++MD++++P P+ FNPSRW+      G F+
Sbjct: 384 FVSFRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRWEGPPPKAGTFL 443

Query: 433 PFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPKDKCLARVHKV 491
           PFG G+R CPG+DLAKLEIS+FLH+FLL YK+++ NPKC + Y+P P P D CLA + KV
Sbjct: 444 PFGLGARLCPGNDLAKLEISVFLHHFLLGYKLKRANPKCRVRYLPHPRPVDNCLATITKV 502

BLAST of Csor.00g188270 vs. ExPASy Swiss-Prot
Match: Q43246 (Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 8.2e-117
Identity = 206/476 (43.28%), Postives = 317/476 (66.60%), Query Frame = 0

Query: 16  AFVFVLGILKRVNGWYYAAKLGKT-WSMLPPGDMGWPLLGSTISYIKSFISGQPSNFIAN 75
           A V V    +R +GWY  A LG    + LPPG+MGWPL+G   +++++F SG+P  FIA+
Sbjct: 44  ALVVVDAAARRAHGWYREAALGAARRARLPPGEMGWPLVGGMWAFLRAFKSGKPDAFIAS 103

Query: 76  FYIRYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKNINMLTGRKSLVQVF 135
           F  R+G+  +Y++ +    T++V ++E C+QVL D+D F    PK    L G +S V + 
Sbjct: 104 FVRRFGRTGVYRSFMFSSPTVLVTTAEGCKQVLMDDDAFVTGWPKATVALVGPRSFVAMP 163

Query: 136 KAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIEVLSEIKKLTFKII 195
             EH+R+R+LT API+G  AL  Y+  I+ TV S L  WA     +E L+E++++TFKII
Sbjct: 164 YDEHRRIRKLTAAPINGFDALTGYLPFIDRTVTSSLRAWADHGGSVEFLTELRRMTFKII 223

Query: 196 WNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARKKLLNILQSIINQ 255
             IF+G +  +++   +E  ++E+  G  ++ IN PGF    AL+AR++L+ +LQ ++++
Sbjct: 224 VQIFLGGAD-QATTRALERSYTELNYGMRAMAINLPGFAYRGALRARRRLVAVLQGVLDE 283

Query: 256 KRLVRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLFAGHETSAYTAMWAV 315
           +R  R + G S    DMMD L+E +DE G  LDD+ I D++   L AGHE+S +  MWA 
Sbjct: 284 RRAARAK-GVSGGGVDMMDRLIEAQDERGRHLDDDEIIDVLVMYLNAGHESSGHITMWAT 343

Query: 316 LFLTKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVIDETLRVGGVTLMLF 375
           +FL ++P +F RAK EQE I+   P++Q+G+ L + ++M++L QVIDETLR+  ++ + F
Sbjct: 344 VFLQENPDMFARAKAEQEAIMRSIPSSQRGLTLRDFRKMEYLSQVIDETLRLVNISFVSF 403

Query: 376 REAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWDNYVATPGAFIPFGS 435
           R+A  DV +NG +IPKGWKV  W+  ++MD +++P P +F+PSRW+ +    G F+ FG 
Sbjct: 404 RQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPTKFDPSRWEGHSPRAGTFLAFGL 463

Query: 436 GSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPKDKCLARVHKV 491
           G+R CPG+DLAKLEIS+FLH+FLL YK+ + NP+C + Y+P P P D CLA++ +V
Sbjct: 464 GARLCPGNDLAKLEISVFLHHFLLGYKLARTNPRCRVRYLPHPRPVDNCLAKITRV 517

BLAST of Csor.00g188270 vs. NCBI nr
Match: XP_022923278.1 (beta-amyrin 11-oxidase-like isoform X1 [Cucurbita moschata] >KAG6577626.1 Beta-amyrin 11-oxidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 988 bits (2553), Expect = 0.0
Identity = 490/490 (100.00%), Postives = 490/490 (100.00%), Query Frame = 0

Query: 1   MLTMGVKNWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWSMLPPGDMGWPLLGSTISYI 60
           MLTMGVKNWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWSMLPPGDMGWPLLGSTISYI
Sbjct: 1   MLTMGVKNWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWSMLPPGDMGWPLLGSTISYI 60

Query: 61  KSFISGQPSNFIANFYIRYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKN 120
           KSFISGQPSNFIANFYIRYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKN
Sbjct: 61  KSFISGQPSNFIANFYIRYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKN 120

Query: 121 INMLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPI 180
           INMLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPI
Sbjct: 121 INMLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPI 180

Query: 181 EVLSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKA 240
           EVLSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKA
Sbjct: 181 EVLSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKA 240

Query: 241 RKKLLNILQSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLF 300
           RKKLLNILQSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLF
Sbjct: 241 RKKLLNILQSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLF 300

Query: 301 AGHETSAYTAMWAVLFLTKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVI 360
           AGHETSAYTAMWAVLFLTKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVI
Sbjct: 301 AGHETSAYTAMWAVLFLTKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVI 360

Query: 361 DETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWD 420
           DETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWD
Sbjct: 361 DETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWD 420

Query: 421 NYVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPK 480
           NYVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPK
Sbjct: 421 NYVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPK 480

Query: 481 DKCLARVHKV 490
           DKCLARVHKV
Sbjct: 481 DKCLARVHKV 490

BLAST of Csor.00g188270 vs. NCBI nr
Match: XP_023552542.1 (beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 962 bits (2488), Expect = 0.0
Identity = 476/490 (97.14%), Postives = 483/490 (98.57%), Query Frame = 0

Query: 1   MLTMGVKNWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWSMLPPGDMGWPLLGSTISYI 60
           ML M VKNWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTW  LPPG MGWPLLGSTISYI
Sbjct: 1   MLAMEVKNWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWPKLPPGHMGWPLLGSTISYI 60

Query: 61  KSFISGQPSNFIANFYIRYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKN 120
           K+FISGQPSNF+ANFYIRYGK DMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKN
Sbjct: 61  KNFISGQPSNFMANFYIRYGKSDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKN 120

Query: 121 INMLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPI 180
           INMLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPI
Sbjct: 121 INMLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPI 180

Query: 181 EVLSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKA 240
           E+LSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKA
Sbjct: 181 ELLSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKA 240

Query: 241 RKKLLNILQSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLF 300
           RKKLL+ILQSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLF
Sbjct: 241 RKKLLDILQSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLF 300

Query: 301 AGHETSAYTAMWAVLFLTKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVI 360
           AGHETSAYTAMWAVLFLTKHPHIFQRAKEEQ+RI+TQRPTTQKGIILSEVKQMKFL Q+I
Sbjct: 301 AGHETSAYTAMWAVLFLTKHPHIFQRAKEEQDRIITQRPTTQKGIILSEVKQMKFLSQII 360

Query: 361 DETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWD 420
           DETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWD
Sbjct: 361 DETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWD 420

Query: 421 NYVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPK 480
           NYVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPK
Sbjct: 421 NYVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPK 480

Query: 481 DKCLARVHKV 490
           DKCLARVHKV
Sbjct: 481 DKCLARVHKV 490

BLAST of Csor.00g188270 vs. NCBI nr
Match: XP_023007796.1 (beta-amyrin 11-oxidase-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 954 bits (2466), Expect = 0.0
Identity = 472/490 (96.33%), Postives = 482/490 (98.37%), Query Frame = 0

Query: 1   MLTMGVKNWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWSMLPPGDMGWPLLGSTISYI 60
           ML M VKNWLVLIVAAFVFVLGILKR+NGWYYAAKLGKTW  LPPGDMGWPLLGSTISYI
Sbjct: 1   MLAMEVKNWLVLIVAAFVFVLGILKRLNGWYYAAKLGKTWPKLPPGDMGWPLLGSTISYI 60

Query: 61  KSFISGQPSNFIANFYIRYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKN 120
           K+FISGQPSNFIANFYIRYGK DMYKTH+LGRATIIVCSSELCRQVLGDEDNFKPSLPKN
Sbjct: 61  KNFISGQPSNFIANFYIRYGKRDMYKTHLLGRATIIVCSSELCRQVLGDEDNFKPSLPKN 120

Query: 121 INMLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPI 180
           INMLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPI
Sbjct: 121 INMLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPI 180

Query: 181 EVLSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKA 240
           E+LSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKA
Sbjct: 181 ELLSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKA 240

Query: 241 RKKLLNILQSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLF 300
           RKKLL+ILQSII+QKRLVR+RQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLF
Sbjct: 241 RKKLLDILQSIIDQKRLVRKRQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLF 300

Query: 301 AGHETSAYTAMWAVLFLTKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVI 360
           AGHETSAYTAMWAVLFLTKHPHIFQRAKEEQE IV QRPTTQKGIILSEVK+MKFL Q+I
Sbjct: 301 AGHETSAYTAMWAVLFLTKHPHIFQRAKEEQELIVAQRPTTQKGIILSEVKRMKFLSQII 360

Query: 361 DETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWD 420
           DETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSP+EFNPSRWD
Sbjct: 361 DETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPREFNPSRWD 420

Query: 421 NYVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPK 480
           NYVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPK
Sbjct: 421 NYVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPK 480

Query: 481 DKCLARVHKV 490
           DKCLARVHKV
Sbjct: 481 DKCLARVHKV 490

BLAST of Csor.00g188270 vs. NCBI nr
Match: XP_023552543.1 (beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 851 bits (2199), Expect = 2.54e-309
Identity = 418/490 (85.31%), Postives = 449/490 (91.63%), Query Frame = 0

Query: 1   MLTMGVKNWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWSMLPPGDMGWPLLGSTISYI 60
           ML M VKNWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTW  LPPG MGWPLLGSTI ++
Sbjct: 1   MLAMEVKNWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWPKLPPGHMGWPLLGSTILFL 60

Query: 61  KSFISGQPSNFIANFYIRYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKN 120
           KSF SGQP NFI +FYIRYGKGDMYKTHILGRATIIVC+SELCRQVLGDE NFKPS P +
Sbjct: 61  KSFSSGQPRNFIRSFYIRYGKGDMYKTHILGRATIIVCTSELCRQVLGDEHNFKPSFPVS 120

Query: 121 INMLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPI 180
           IN L+G+KSL+QVFKAEHKRLRRLTMAPISGH AL+MYINHIEHTV++GLEEWASMKNPI
Sbjct: 121 INRLSGKKSLIQVFKAEHKRLRRLTMAPISGHMALKMYINHIEHTVINGLEEWASMKNPI 180

Query: 181 EVLSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKA 240
           E+LSE+KKLTF+IIWNIFMGSSS E SI EM  LFS+VALGFISLPINFPGFN HKALKA
Sbjct: 181 ELLSEMKKLTFQIIWNIFMGSSSFEYSIDEMVVLFSQVALGFISLPINFPGFNFHKALKA 240

Query: 241 RKKLLNILQSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLF 300
           RKKLL+ILQSIINQKRLV+  QG SWEAKDMMDLL+EVKDEDGE LDDETI DLIFG LF
Sbjct: 241 RKKLLHILQSIINQKRLVKNMQGKSWEAKDMMDLLIEVKDEDGEALDDETIIDLIFGNLF 300

Query: 301 AGHETSAYTAMWAVLFLTKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVI 360
           AGHETS+ TAMWAVLFL+KHPHIFQ AKEEQE IVTQRPTTQKGI LSE+KQMKFLYQVI
Sbjct: 301 AGHETSSITAMWAVLFLSKHPHIFQTAKEEQEHIVTQRPTTQKGITLSEIKQMKFLYQVI 360

Query: 361 DETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWD 420
           DETLR+GGVT M+FREA+ DVEINGKIIPKGWKVIPWFGE ++DEK FPSPQEFNPSRWD
Sbjct: 361 DETLRLGGVTFMVFREALADVEINGKIIPKGWKVIPWFGEHFIDEKYFPSPQEFNPSRWD 420

Query: 421 NYVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPK 480
           NYVATPG FIPFGSGSR+CPGSDLAK E+SIFLHYFLLNYKIE LNP C +T +PFPHPK
Sbjct: 421 NYVATPGVFIPFGSGSRHCPGSDLAKFEVSIFLHYFLLNYKIELLNPNCELTCLPFPHPK 480

Query: 481 DKCLARVHKV 490
           DK LARV+KV
Sbjct: 481 DKGLARVYKV 490

BLAST of Csor.00g188270 vs. NCBI nr
Match: XP_022923277.1 (beta-amyrin 11-oxidase-like [Cucurbita moschata])

HSP 1 Score: 850 bits (2195), Expect = 1.03e-308
Identity = 415/490 (84.69%), Postives = 447/490 (91.22%), Query Frame = 0

Query: 1   MLTMGVKNWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWSMLPPGDMGWPLLGSTISYI 60
           ML M VKNWLVL+VAAFVFVLG+LKR+NGWYYAAKLGK W  LPPGDMGWPLLGSTI ++
Sbjct: 1   MLAMEVKNWLVLVVAAFVFVLGVLKRLNGWYYAAKLGKAWPKLPPGDMGWPLLGSTILFL 60

Query: 61  KSFISGQPSNFIANFYIRYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKN 120
           KSF  GQP NFI+N +IRYGKGDMYKTHILGRATIIVC+SELCRQVLGDE NFKPS P +
Sbjct: 61  KSFSCGQPRNFISNIFIRYGKGDMYKTHILGRATIIVCTSELCRQVLGDEHNFKPSFPVS 120

Query: 121 INMLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPI 180
           IN L+GRKSL+QVFKAEH+RLRRLTMAPISGHAAL+MYINHIEHTVMSGLEEWASMKNPI
Sbjct: 121 INRLSGRKSLIQVFKAEHRRLRRLTMAPISGHAALKMYINHIEHTVMSGLEEWASMKNPI 180

Query: 181 EVLSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKA 240
           E+LSE+KKLTFKIIWNIFMGSSS E SI EM  LFSEVALGFISLPINFPGFN HK+LKA
Sbjct: 181 ELLSEMKKLTFKIIWNIFMGSSSFEYSIDEMVVLFSEVALGFISLPINFPGFNFHKSLKA 240

Query: 241 RKKLLNILQSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLF 300
           RKKLL ILQSII+QKRLV+  QG SWEAKDMMDLLM+VKDEDGEELDDETI DLIFG LF
Sbjct: 241 RKKLLKILQSIIHQKRLVKNMQGKSWEAKDMMDLLMDVKDEDGEELDDETIIDLIFGNLF 300

Query: 301 AGHETSAYTAMWAVLFLTKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVI 360
           AGHETS+ TAMWAVLFLTKH HIFQ AKEEQERIV QRPTTQKGI +SE+KQMKFLYQVI
Sbjct: 301 AGHETSSITAMWAVLFLTKHSHIFQTAKEEQERIVAQRPTTQKGITISEIKQMKFLYQVI 360

Query: 361 DETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWD 420
           DETLR+GGVT M+FREA+ DVEINGKIIPKGWKVIPWFGE ++DEK FPSPQEFNPSRWD
Sbjct: 361 DETLRLGGVTFMVFREALADVEINGKIIPKGWKVIPWFGEHFIDEKYFPSPQEFNPSRWD 420

Query: 421 NYVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPK 480
           NYVATPG FIPFGSGSR+CPGSDLAK E+SIFLHYFLLNYK+E LNP C  T +PFPHPK
Sbjct: 421 NYVATPGVFIPFGSGSRHCPGSDLAKFEVSIFLHYFLLNYKVELLNPNCKSTCLPFPHPK 480

Query: 481 DKCLARVHKV 490
           DK LARV+KV
Sbjct: 481 DKGLARVYKV 490

BLAST of Csor.00g188270 vs. ExPASy TrEMBL
Match: A0A6J1E938 (beta-amyrin 11-oxidase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431020 PE=3 SV=1)

HSP 1 Score: 988 bits (2553), Expect = 0.0
Identity = 490/490 (100.00%), Postives = 490/490 (100.00%), Query Frame = 0

Query: 1   MLTMGVKNWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWSMLPPGDMGWPLLGSTISYI 60
           MLTMGVKNWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWSMLPPGDMGWPLLGSTISYI
Sbjct: 1   MLTMGVKNWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWSMLPPGDMGWPLLGSTISYI 60

Query: 61  KSFISGQPSNFIANFYIRYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKN 120
           KSFISGQPSNFIANFYIRYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKN
Sbjct: 61  KSFISGQPSNFIANFYIRYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKN 120

Query: 121 INMLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPI 180
           INMLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPI
Sbjct: 121 INMLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPI 180

Query: 181 EVLSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKA 240
           EVLSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKA
Sbjct: 181 EVLSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKA 240

Query: 241 RKKLLNILQSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLF 300
           RKKLLNILQSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLF
Sbjct: 241 RKKLLNILQSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLF 300

Query: 301 AGHETSAYTAMWAVLFLTKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVI 360
           AGHETSAYTAMWAVLFLTKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVI
Sbjct: 301 AGHETSAYTAMWAVLFLTKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVI 360

Query: 361 DETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWD 420
           DETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWD
Sbjct: 361 DETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWD 420

Query: 421 NYVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPK 480
           NYVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPK
Sbjct: 421 NYVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPK 480

Query: 481 DKCLARVHKV 490
           DKCLARVHKV
Sbjct: 481 DKCLARVHKV 490

BLAST of Csor.00g188270 vs. ExPASy TrEMBL
Match: A0A6J1L1N7 (beta-amyrin 11-oxidase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111500298 PE=3 SV=1)

HSP 1 Score: 954 bits (2466), Expect = 0.0
Identity = 472/490 (96.33%), Postives = 482/490 (98.37%), Query Frame = 0

Query: 1   MLTMGVKNWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWSMLPPGDMGWPLLGSTISYI 60
           ML M VKNWLVLIVAAFVFVLGILKR+NGWYYAAKLGKTW  LPPGDMGWPLLGSTISYI
Sbjct: 1   MLAMEVKNWLVLIVAAFVFVLGILKRLNGWYYAAKLGKTWPKLPPGDMGWPLLGSTISYI 60

Query: 61  KSFISGQPSNFIANFYIRYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKN 120
           K+FISGQPSNFIANFYIRYGK DMYKTH+LGRATIIVCSSELCRQVLGDEDNFKPSLPKN
Sbjct: 61  KNFISGQPSNFIANFYIRYGKRDMYKTHLLGRATIIVCSSELCRQVLGDEDNFKPSLPKN 120

Query: 121 INMLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPI 180
           INMLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPI
Sbjct: 121 INMLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPI 180

Query: 181 EVLSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKA 240
           E+LSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKA
Sbjct: 181 ELLSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKA 240

Query: 241 RKKLLNILQSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLF 300
           RKKLL+ILQSII+QKRLVR+RQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLF
Sbjct: 241 RKKLLDILQSIIDQKRLVRKRQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLF 300

Query: 301 AGHETSAYTAMWAVLFLTKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVI 360
           AGHETSAYTAMWAVLFLTKHPHIFQRAKEEQE IV QRPTTQKGIILSEVK+MKFL Q+I
Sbjct: 301 AGHETSAYTAMWAVLFLTKHPHIFQRAKEEQELIVAQRPTTQKGIILSEVKRMKFLSQII 360

Query: 361 DETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWD 420
           DETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSP+EFNPSRWD
Sbjct: 361 DETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPREFNPSRWD 420

Query: 421 NYVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPK 480
           NYVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPK
Sbjct: 421 NYVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPK 480

Query: 481 DKCLARVHKV 490
           DKCLARVHKV
Sbjct: 481 DKCLARVHKV 490

BLAST of Csor.00g188270 vs. ExPASy TrEMBL
Match: A0A6J1E5Q1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111431019 PE=3 SV=1)

HSP 1 Score: 850 bits (2195), Expect = 4.99e-309
Identity = 415/490 (84.69%), Postives = 447/490 (91.22%), Query Frame = 0

Query: 1   MLTMGVKNWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWSMLPPGDMGWPLLGSTISYI 60
           ML M VKNWLVL+VAAFVFVLG+LKR+NGWYYAAKLGK W  LPPGDMGWPLLGSTI ++
Sbjct: 1   MLAMEVKNWLVLVVAAFVFVLGVLKRLNGWYYAAKLGKAWPKLPPGDMGWPLLGSTILFL 60

Query: 61  KSFISGQPSNFIANFYIRYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKN 120
           KSF  GQP NFI+N +IRYGKGDMYKTHILGRATIIVC+SELCRQVLGDE NFKPS P +
Sbjct: 61  KSFSCGQPRNFISNIFIRYGKGDMYKTHILGRATIIVCTSELCRQVLGDEHNFKPSFPVS 120

Query: 121 INMLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPI 180
           IN L+GRKSL+QVFKAEH+RLRRLTMAPISGHAAL+MYINHIEHTVMSGLEEWASMKNPI
Sbjct: 121 INRLSGRKSLIQVFKAEHRRLRRLTMAPISGHAALKMYINHIEHTVMSGLEEWASMKNPI 180

Query: 181 EVLSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKA 240
           E+LSE+KKLTFKIIWNIFMGSSS E SI EM  LFSEVALGFISLPINFPGFN HK+LKA
Sbjct: 181 ELLSEMKKLTFKIIWNIFMGSSSFEYSIDEMVVLFSEVALGFISLPINFPGFNFHKSLKA 240

Query: 241 RKKLLNILQSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLF 300
           RKKLL ILQSII+QKRLV+  QG SWEAKDMMDLLM+VKDEDGEELDDETI DLIFG LF
Sbjct: 241 RKKLLKILQSIIHQKRLVKNMQGKSWEAKDMMDLLMDVKDEDGEELDDETIIDLIFGNLF 300

Query: 301 AGHETSAYTAMWAVLFLTKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVI 360
           AGHETS+ TAMWAVLFLTKH HIFQ AKEEQERIV QRPTTQKGI +SE+KQMKFLYQVI
Sbjct: 301 AGHETSSITAMWAVLFLTKHSHIFQTAKEEQERIVAQRPTTQKGITISEIKQMKFLYQVI 360

Query: 361 DETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWD 420
           DETLR+GGVT M+FREA+ DVEINGKIIPKGWKVIPWFGE ++DEK FPSPQEFNPSRWD
Sbjct: 361 DETLRLGGVTFMVFREALADVEINGKIIPKGWKVIPWFGEHFIDEKYFPSPQEFNPSRWD 420

Query: 421 NYVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPK 480
           NYVATPG FIPFGSGSR+CPGSDLAK E+SIFLHYFLLNYK+E LNP C  T +PFPHPK
Sbjct: 421 NYVATPGVFIPFGSGSRHCPGSDLAKFEVSIFLHYFLLNYKVELLNPNCKSTCLPFPHPK 480

Query: 481 DKCLARVHKV 490
           DK LARV+KV
Sbjct: 481 DKGLARVYKV 490

BLAST of Csor.00g188270 vs. ExPASy TrEMBL
Match: A0A6J1L8N2 (beta-amyrin 11-oxidase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111500299 PE=3 SV=1)

HSP 1 Score: 841 bits (2172), Expect = 1.53e-305
Identity = 415/490 (84.69%), Postives = 444/490 (90.61%), Query Frame = 0

Query: 1   MLTMGVKNWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWSMLPPGDMGWPLLGSTISYI 60
           ML M VKNWLVLIVAAFVFVLGILKR+NGWYYAAKLGKTW  LPPG MGWPLLGSTI ++
Sbjct: 1   MLAMEVKNWLVLIVAAFVFVLGILKRLNGWYYAAKLGKTWPKLPPGHMGWPLLGSTILFL 60

Query: 61  KSFISGQPSNFIANFYIRYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKN 120
           KSF SGQP NFI +FYIRYGK DMYKTHILGRATIIVC+SELCRQVLGDE NFKPS P +
Sbjct: 61  KSFSSGQPRNFIRSFYIRYGKCDMYKTHILGRATIIVCTSELCRQVLGDEHNFKPSFPVS 120

Query: 121 INMLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPI 180
           IN LTGRKSL+QVFKAEHKRLRRLT APISGHAAL+MYIN IEHTVMSGLEEWASMKNPI
Sbjct: 121 INRLTGRKSLIQVFKAEHKRLRRLTTAPISGHAALKMYINGIEHTVMSGLEEWASMKNPI 180

Query: 181 EVLSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKA 240
           E+LSE+KKLTFKIIWNIFMGSS LE SI EM  LFS+VA+GFISLPIN PGFN H++LKA
Sbjct: 181 ELLSEMKKLTFKIIWNIFMGSS-LEYSIDEMVVLFSQVAIGFISLPINSPGFNFHQSLKA 240

Query: 241 RKKLLNILQSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLF 300
           RKKLLNILQSIINQKRL +  QG SWEAKDMMDLLM+VKDEDGEELDDETI DLIFG LF
Sbjct: 241 RKKLLNILQSIINQKRLAKNMQGKSWEAKDMMDLLMDVKDEDGEELDDETIIDLIFGNLF 300

Query: 301 AGHETSAYTAMWAVLFLTKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVI 360
           AGHETS+ TAMWAVLFLTKHPH+FQRAKEEQE IVTQRPTTQKGI +S +KQMKFLYQVI
Sbjct: 301 AGHETSSITAMWAVLFLTKHPHVFQRAKEEQEHIVTQRPTTQKGITISNIKQMKFLYQVI 360

Query: 361 DETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWD 420
           DETLR  GVT M+FREA+ DVEINGKIIPKGWKVIPWFGEL+ DEK FPSPQEFNPSRWD
Sbjct: 361 DETLRASGVTFMVFREALADVEINGKIIPKGWKVIPWFGELFTDEKYFPSPQEFNPSRWD 420

Query: 421 NYVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPK 480
           NYVATPG FIPFGSGSR+CPGSDLAK E+SIFLHYFLLNYK+E LNP C +T +PFPHPK
Sbjct: 421 NYVATPGVFIPFGSGSRHCPGSDLAKFEVSIFLHYFLLNYKVELLNPNCKLTCLPFPHPK 480

Query: 481 DKCLARVHKV 490
           DK LARV+KV
Sbjct: 481 DKGLARVYKV 489

BLAST of Csor.00g188270 vs. ExPASy TrEMBL
Match: A0A6J1E5Y2 (beta-amyrin 11-oxidase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111431020 PE=3 SV=1)

HSP 1 Score: 833 bits (2153), Expect = 1.89e-303
Identity = 413/413 (100.00%), Postives = 413/413 (100.00%), Query Frame = 0

Query: 78  RYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKNINMLTGRKSLVQVFKAE 137
           RYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKNINMLTGRKSLVQVFKAE
Sbjct: 29  RYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKNINMLTGRKSLVQVFKAE 88

Query: 138 HKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIEVLSEIKKLTFKIIWNI 197
           HKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIEVLSEIKKLTFKIIWNI
Sbjct: 89  HKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIEVLSEIKKLTFKIIWNI 148

Query: 198 FMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARKKLLNILQSIINQKRL 257
           FMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARKKLLNILQSIINQKRL
Sbjct: 149 FMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARKKLLNILQSIINQKRL 208

Query: 258 VRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLFAGHETSAYTAMWAVLFL 317
           VRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLFAGHETSAYTAMWAVLFL
Sbjct: 209 VRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLFAGHETSAYTAMWAVLFL 268

Query: 318 TKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVIDETLRVGGVTLMLFREA 377
           TKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVIDETLRVGGVTLMLFREA
Sbjct: 269 TKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVIDETLRVGGVTLMLFREA 328

Query: 378 VVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWDNYVATPGAFIPFGSGSR 437
           VVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWDNYVATPGAFIPFGSGSR
Sbjct: 329 VVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWDNYVATPGAFIPFGSGSR 388

Query: 438 YCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPKDKCLARVHKV 490
           YCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPKDKCLARVHKV
Sbjct: 389 YCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPKDKCLARVHKV 441

BLAST of Csor.00g188270 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 470.7 bits (1210), Expect = 1.4e-132
Identity = 232/486 (47.74%), Postives = 330/486 (67.90%), Query Frame = 0

Query: 3   TMGVKNWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWSMLPPGDMGWPLLGSTISYIKS 62
           T  +  W  L+V     +  ++++VN W Y + LG+    LPPGD+GWP +G+ +S++++
Sbjct: 5   TSWIPVWFPLMVLGCFGLNWLVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRA 64

Query: 63  FISGQPSNFIANFYIRYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKNIN 122
           F +  P +F      RYG   +YK H+ G  +IIV +S+ CR+VL D+D FKP  P +  
Sbjct: 65  FKTSDPDSFTRTLIKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTM 124

Query: 123 MLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIEV 182
            L GRKS V +   EHKRLRRLT AP++GH AL  YI +IE  V++ L++W  M    E 
Sbjct: 125 ELIGRKSFVGISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKM-GEFEF 184

Query: 183 LSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARK 242
           L+ ++KLTF+II  IF+ S S E+ +  +E  ++ +  G  ++ +N PGF  H+ALKARK
Sbjct: 185 LTHLRKLTFRIIMYIFLSSES-ENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARK 244

Query: 243 KLLNILQSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLFAG 302
            L+   QSI+ ++R  R +Q      KDM+D L+ VKDEDG+ LDDE I D++   L AG
Sbjct: 245 TLVAAFQSIVTERRNQR-KQNILSNKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAG 304

Query: 303 HETSAYTAMWAVLFLTKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVIDE 362
           HE+S +T MWA +FL +HP + QRAK EQE I+  RP  QKG+ L E ++M+FL QV+DE
Sbjct: 305 HESSGHTIMWATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDE 364

Query: 363 TLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWDN- 422
           TLRV   +L  FREA  DVE+NG +IPKGWKV+ WF ++++D ++FP P++F+P+RWDN 
Sbjct: 365 TLRVITFSLTAFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNG 424

Query: 423 YVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPKD 482
           +V   GAF+PFG+GS  CPG+DLAKLEISIFLH+FLL Y++++ NP+CP+ Y+P   P D
Sbjct: 425 FVPKAGAFLPFGAGSHLCPGNDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTD 484

Query: 483 KCLARV 488
            CLAR+
Sbjct: 485 NCLARI 487

BLAST of Csor.00g188270 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 465.7 bits (1197), Expect = 4.6e-131
Identity = 234/479 (48.85%), Postives = 324/479 (67.64%), Query Frame = 0

Query: 9   WLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWSMLPPGDMGWPLLGSTISYIKSFISGQP 68
           W  LI+     +  +LKRVN W Y +KLG+    LPPGD+GWP++G+  S++++F +  P
Sbjct: 10  WFPLIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDP 69

Query: 69  SNFIANFYIRYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKNINMLTGRK 128
            +FI ++  RYG+  +YK H+ G   ++V + E CR+VL D+D F    PK+   L GRK
Sbjct: 70  ESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRK 129

Query: 129 SLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIEVLSEIKK 188
           S V +   EHKRLRRLT AP++G  AL +YI  IE TV + LE+W+ M   IE LS ++K
Sbjct: 130 SFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKM-GEIEFLSHLRK 189

Query: 189 LTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARKKLLNIL 248
           LTFK+I  IF+ S S E  +  +E  ++ +  G  ++ IN PGF  H+ALKARKKL+   
Sbjct: 190 LTFKVIMYIFLSSES-EHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAF 249

Query: 249 QSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLFAGHETSAY 308
           QSI+  +R  R +Q  S   KDM+D L++VKDE+G  LDDE I DL+   L AGHE+S +
Sbjct: 250 QSIVTNRRNQR-KQNISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGH 309

Query: 309 TAMWAVLFLTKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVIDETLRVGG 368
             MWA + + +HP I Q+AKEEQERIV +R   QK + L E ++M +L QVIDETLRV  
Sbjct: 310 LTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVIT 369

Query: 369 VTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWDNYVATPGA 428
            +L  FREA  DV+++G IIPKGWKV+ WF  +++D +++P P++F+PSRW+ Y    G 
Sbjct: 370 FSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGT 429

Query: 429 FIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPKDKCLARV 488
           F+PFG GS  CPG+DLAKLEISIFLH+FLL Y++E+ NP CP+ ++P   PKD CLAR+
Sbjct: 430 FLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARI 484

BLAST of Csor.00g188270 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 465.7 bits (1197), Expect = 4.6e-131
Identity = 234/479 (48.85%), Postives = 324/479 (67.64%), Query Frame = 0

Query: 9   WLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWSMLPPGDMGWPLLGSTISYIKSFISGQP 68
           W  LI+     +  +LKRVN W Y +KLG+    LPPGD+GWP++G+  S++++F +  P
Sbjct: 10  WFPLIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDP 69

Query: 69  SNFIANFYIRYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKNINMLTGRK 128
            +FI ++  RYG+  +YK H+ G   ++V + E CR+VL D+D F    PK+   L GRK
Sbjct: 70  ESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRK 129

Query: 129 SLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIEVLSEIKK 188
           S V +   EHKRLRRLT AP++G  AL +YI  IE TV + LE+W+ M   IE LS ++K
Sbjct: 130 SFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKM-GEIEFLSHLRK 189

Query: 189 LTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARKKLLNIL 248
           LTFK+I  IF+ S S E  +  +E  ++ +  G  ++ IN PGF  H+ALKARKKL+   
Sbjct: 190 LTFKVIMYIFLSSES-EHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAF 249

Query: 249 QSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLFAGHETSAY 308
           QSI+  +R  R +Q  S   KDM+D L++VKDE+G  LDDE I DL+   L AGHE+S +
Sbjct: 250 QSIVTNRRNQR-KQNISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGH 309

Query: 309 TAMWAVLFLTKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVIDETLRVGG 368
             MWA + + +HP I Q+AKEEQERIV +R   QK + L E ++M +L QVIDETLRV  
Sbjct: 310 LTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVIT 369

Query: 369 VTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWDNYVATPGA 428
            +L  FREA  DV+++G IIPKGWKV+ WF  +++D +++P P++F+PSRW+ Y    G 
Sbjct: 370 FSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGT 429

Query: 429 FIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPKDKCLARV 488
           F+PFG GS  CPG+DLAKLEISIFLH+FLL Y++E+ NP CP+ ++P   PKD CLAR+
Sbjct: 430 FLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARI 484

BLAST of Csor.00g188270 vs. TAIR 10
Match: AT3G19270.1 (cytochrome P450, family 707, subfamily A, polypeptide 4 )

HSP 1 Score: 247.7 bits (631), Expect = 2.0e-65
Identity = 157/441 (35.60%), Postives = 235/441 (53.29%), Query Frame = 0

Query: 43  LPPGDMGWPLLGSTISYIKSFISGQPSNFIANFYIRYGKGDMYKTHILGRATIIVCSSEL 102
           LPPG MGWP LG T+       S  P+ F  +   RY  G+++KT ILG   +++ S E 
Sbjct: 34  LPPGSMGWPYLGETL----QLYSQNPNVFFTSKQKRY--GEIFKTRILGYPCVMLASPEA 93

Query: 103 CRQVLGDEDN-FKPSLPKNINMLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINH 162
            R VL    + FKP+ P++   L G  +L       H  +R+L  +       +   I  
Sbjct: 94  ARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGDYHSHIRKLVQSSFYPE-TIRKLIPD 153

Query: 163 IEHTVMSGLEEWASMKNPIEVLSEIKKLTFKIIWNIFMGSSSLESSIGE-MEGLFSEVAL 222
           IEH  +S L+ WA+M   +    E+KK  F +   I      LESS  E ++  ++ V  
Sbjct: 154 IEHIALSSLQSWANMP-IVSTYQEMKKFAFDV--GILAIFGHLESSYKEILKHNYNIVDK 213

Query: 223 GFISLPINFPGFNLHKALKARKKLLNILQSIINQKRLVRERQGTSWEAKDMMDLLMEVKD 282
           G+ S P++ PG + HKAL ARK+L  I+  II ++R  R  Q       D +  L+  K+
Sbjct: 214 GYNSFPMSLPGTSYHKALMARKQLKTIVSEIICERREKRALQ------TDFLGHLLNFKN 273

Query: 283 EDGEELDDETIRDLIFGKLFAGHETSAYTAMWAVLFLTKHPHIFQRAKEEQERIVTQRPT 342
           E G  L  E I D I G LFA  +T+A    W + +L     + +  K EQ+ I  +   
Sbjct: 274 EKGRVLTQEQIADNIIGVLFAAQDTTASCLTWILKYLHDDQKLLEAVKAEQKAIYEENSR 333

Query: 343 TQKGIILSEVKQMKFLYQVIDETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGE 402
            +K +   + + M   ++VI E+LR+  +    FREAVVDVE  G +IPKGWKV+P F  
Sbjct: 334 EKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRN 393

Query: 403 LYMDEKLFPSPQEFNPSRWDNYVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNY 462
           ++ + K F +P+ F+PSR++     P  F+PFGSG   CPG++LAKL+I IFLH+ + N+
Sbjct: 394 IHHNPKYFSNPEVFDPSRFE-VNPKPNTFMPFGSGVHACPGNELAKLQILIFLHHLVSNF 453

Query: 463 KIEQLNPKCPMTYMPFPHPKD 482
           + E    +  + Y PFP P++
Sbjct: 454 RWEVKGGEKGIQYSPFPIPQN 457

BLAST of Csor.00g188270 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 246.5 bits (628), Expect = 4.4e-65
Identity = 149/446 (33.41%), Postives = 236/446 (52.91%), Query Frame = 0

Query: 43  LPPGDMGWPLLGSTISYIKSFISGQPSNFIANFYIRYGKGDMYKTHILGRATIIVCSSEL 102
           LPPG MG P +G T+       +  P++F A    +Y  GD++KTHILG   +++ S E 
Sbjct: 49  LPPGSMGLPYIGETL----RLYTENPNSFFATRQNKY--GDIFKTHILGCPCVMISSPEA 108

Query: 103 CRQVLGDEDN-FKPSLPKNINMLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINH 162
            R VL  + + FKP+ P +   + G ++L       H  L+RL  +     +AL   ++H
Sbjct: 109 ARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFM-PSALRPTVSH 168

Query: 163 IEHTVMSGLEEWASMKNPIEVLSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALG 222
           IE  V+  L  W S K+ I  L  +K+  F +      G     ++I  ++ L+  +  G
Sbjct: 169 IELLVLQTLSSWTSQKS-INTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERG 228

Query: 223 FISLPINFPGFNLHKALKARKKLLNILQSIINQKRLVRERQGTSWEAKDMMDLLMEVKDE 282
           + S+P++ PG   HK++KAR +L   L+ +I ++R     +G       ++ +L+  KD+
Sbjct: 229 YNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGREEG------GLLGVLLGAKDQ 288

Query: 283 DGEELDDETIRDLIFGKLFAGHETSAYTAMWAVLFLTKHPHIFQRAKEEQERIVTQRPTT 342
               L D  I D I G +FA  +T+A    W + +L  HP++ Q    EQ  I  +    
Sbjct: 289 KRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKE 348

Query: 343 QKGIILSEVKQMKFLYQVIDETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGEL 402
            + I   + ++M    +VI ETLR   V    FREAV DVE +G +IPKGWKV+P F  +
Sbjct: 349 NRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRI 408

Query: 403 YMDEKLFPSPQEFNPSRWDNYVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYK 462
           +   + FP P++F+PSR++     P  ++PFG+G   CPGS+LAKLE+ I LH+   +++
Sbjct: 409 HHSSEFFPDPEKFDPSRFE-VAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFR 468

Query: 463 IEQLNPKCPMTYMPFPHPKDKCLARV 488
            E +  +  + Y PFP PK     RV
Sbjct: 469 WEVIGDEEGIQYGPFPVPKKGLPIRV 479

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
B5BSX17.1e-14550.52Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1[more]
O230512.0e-13147.74Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1[more]
Q9C5Y26.5e-13048.85Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2[more]
Q5VRM74.4e-11843.54Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ... [more]
Q432468.2e-11743.28Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022923278.10.0100.00beta-amyrin 11-oxidase-like isoform X1 [Cucurbita moschata] >KAG6577626.1 Beta-a... [more]
XP_023552542.10.097.14beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo][more]
XP_023007796.10.096.33beta-amyrin 11-oxidase-like isoform X1 [Cucurbita maxima][more]
XP_023552543.12.54e-30985.31beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo][more]
XP_022923277.11.03e-30884.69beta-amyrin 11-oxidase-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1E9380.0100.00beta-amyrin 11-oxidase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
A0A6J1L1N70.096.33beta-amyrin 11-oxidase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1115002... [more]
A0A6J1E5Q14.99e-30984.69beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111431019 PE=3 S... [more]
A0A6J1L8N21.53e-30584.69beta-amyrin 11-oxidase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1115002... [more]
A0A6J1E5Y21.89e-303100.00beta-amyrin 11-oxidase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
Match NameE-valueIdentityDescription
AT1G05160.11.4e-13247.74cytochrome P450, family 88, subfamily A, polypeptide 3 [more]
AT2G32440.14.6e-13148.85ent-kaurenoic acid hydroxylase 2 [more]
AT2G32440.24.6e-13148.85ent-kaurenoic acid hydroxylase 2 [more]
AT3G19270.12.0e-6535.60cytochrome P450, family 707, subfamily A, polypeptide 4 [more]
AT2G29090.14.4e-6533.41cytochrome P450, family 707, subfamily A, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 430..439
score: 56.28
coord: 439..450
score: 39.48
coord: 301..318
score: 34.66
coord: 358..369
score: 36.23
IPR001128Cytochrome P450PFAMPF00067p450coord: 44..470
e-value: 3.8E-69
score: 233.6
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 429..439
score: 53.91
coord: 439..462
score: 30.53
coord: 397..421
score: 27.19
coord: 290..307
score: 25.14
coord: 310..336
score: 27.67
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 36..490
e-value: 1.6E-106
score: 358.7
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 43..487
NoneNo IPR availablePANTHERPTHR24286:SF199CYTOCHROME P450 88D6coord: 22..468
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 22..468
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 432..441

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g188270.m01Csor.00g188270.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen