Csor.00g187130 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g187130
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	Hydroxymethylbilane synthase
Location	Csor_Chr14: 6406606 .. 6409717 (-)
RNA-Seq Expression	Csor.00g187130
Synteny	Csor.00g187130

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGAATCCTATCTGCCAACGCGAATGCAGAGATTTGGCCTGTTGGGTCATCAAAAACAATCCCTTCTAAGCTAACCCATCTCCTTTTTCTTTCTTCTTCTTCTTCGTCTTCTCAAATGGTTGCTTTATCTTCCCCATCTGTAAGCCACTCTCCCATGGCTCACCCTTGTAATCTTGCCTCCCTTTCTCTTCCTGGGTTCTCCTCACTTTCCCTTAAACCTCCGGCTTTCACTAATGGCCCCAAGAAACTTCATGGGTTTGGCCTCATAAGAGCCTCTGTTGCTGTGGAGCAGCAGGCAGGGAAGACCAAGGTTGCTCTCCTCAGAATTGGCACCAGAGGAAGGTATAACTTTTTTGTTTTGTGGGTCTTTTTTAGATTTTCTTTTGGTTTATGTTTTAGGATGTGTTCAGTTTCTAATCATGAGTTTGTGGCTGATTTGAGGGATAATGTAGGGCATTGAGTTGTTGTTTAAGGGTTTGGATCAGTTCAATTTGTGCATTTTAACTGTGATTAAGTTCAGATGAAGTGTTTATGCTGCTCATGCTATCAATCATGGCAGTGATAGTGTTTTACCTTACAATCTAAATGTTTGGATCCAATCTGGTGCTGTTAGAGTAGAATCAGTTCTTCGTTAGAAGAGTAAGTCTATTGTGAGATCCTACGTCGGTTGTGGAGGAGAACGAAGCATTTTTTATAAGGGTGTGGAAACCTCTCCCTAGCATACGCGTTTTAAAATCTTGAGGGGAAGCCCAGAAGTGAAAGTTCAAAGAGGACAATATATGTTAGTGGTGGGATTGGGCCGTTATAAATGGTATTGTCGCCAGACACTGGGTGATGTGCCAGTGGAAGGATGAGCCTCGAAGGAGGGTGGACACGAGGCAGTGTGCCATCAAAGACGTTGGGCCCCAAAGGGGGTGGATTGGGAGATCCCACATCGGTTGAGGAGGACGAAACATTCTCCATAAGAGTGTGGAAACCTCTCCCTAGCAGACACATTCTAAAACCTTGAGGGGAACCCCAAAGAGGACAATATTTACTAGCGATGGCCTTAGGTTGTTACAAATGGTATCAGAGCCAGACACCGGGCAATGTGCAACCGGAAGGCTTAGCCCCAAAGGAGGGAGGACACGAGGCGGTGTGCTAGCAAGGTCATTGGACTCCGAAGGGGTGTGGATTAGGGGTCCCACGTCGATTTGAGAAGGGAACAAGTGCCAGTGAGGACGTTGGGCCCAAAGGGGGTGGATTGTGAGATCCCACATCGGTTGGGGAGGACGAAACATTCTTTATAAGGGTGTGGAAACCTCTTCTAGCAAACGCGTTTTAAAAACCTTAAGGGGAAGCTTAGAAGGAAAAGTCTAAAGAGAACAATATATGCTAGCGGTGGGCTTAGGCGGTTACGTCAAGGCTCGTAGTTTTCTTATCTAAACCTGGAACTATGCCTATAAACACAATGTACTTCATTTTCTATATGCTTGTTTGATTGGGTTTACTTTATCTTCTTCTGTGTCAGGACAGCCCATTAGCACTTGCCCAGGCTCATGAGACAAGAGACAAGCTCATGGCTTCCCATCCTGAGCTAGCTGAAGATGGTGCCATTCAGATTGTTGTTATCAAAACCACTGGTGACAAAATACTCTCTCAGCCACTTGCAGACATTGGTGGGAAAGGCTTATTTACAAAAGAGATTGATGATGCACTTATCAATGGTGACATAGACATTGCTGTTCACTCAATGAAAGATGTCCCAACTTACTTACCTGAGAAAACCATCCTCCCGTGTAACCTTCCGAGGGAGGACGTCCGGGACGCATTCATTTCTCTACGTGCAGGTTCACTTGCCGAGCTTCCAGCTGGAAGCGTCGTCGGTACAGCTTCACTGAGAAGAAAGTCTCAGCTACTGAATCGATATCCGTCCCTCGAAGTAAGTATGGTGTGTTATGTAATGGAAAAACATCCAAAAAGTTTATGTTCTACGTTGACATTGTTGTGGTGTATGTTGAATTCTGATTCTTGATGGAACGTTTATGTGTGAGATCCCATGTTGGTTGGAGAGGTGAACGAAACATTTCTTATAAGGGTGTGGAAACCTCTTCCTAGTAGACACGTTTTAAAATCTTGAGAGGAAGCCCTTGAAGGGAAAGCCCAAAGAGGACAGTATCTACTATCTGTAGGCTTAGACTAGTACAAGTGGTATCAGAGTCAGACACTAGACGGTTTGCTAACGAGGACACTAGGCCCCCAAGGGGGGTGGATTGTAAGATCTAAGCATTCTTTATACACTGGAAACCTCTCACTAGCATACGCGTTTTAAAACCGTGAGGTTGATAGCGATACGTAATGGGCCAAAACAGACAATATCTACTAGCATGGTAGTGTTTAATGAGTCTTAAATCTTCAATCTTGTATGTTGGTTCTGATACTCAATCTCGTTTTTTCCATTGTTTGTTGGTGTCATTTGTTAGGTACTCGAAAATTTTCGAGGTAATGTCCAAACAAGGTTGAAAAAACTAAACAAAGGAGTAGTCCAAGCAACACTATTAGCTCTAGCTGGACTTCGACGTTTAAATATGACAGAAAACGTTACTTCGATCCTTTCAATCGATGAAATGCTTCCAGCTGTTGCTCAGGGAGCTATCGGTATCGCCTGTCGAAGCGACGATGACAAAATGGTGCGTTTTCATTGGCTATACAAATCAATATTTGTCCATGAAAGTTCATTGCTATACAAATCAATATTTGCAGGCTGAGTACTTAGCCTCATTAAACCATGAGGAAACAAGACTGGCTGTTGTTTGTGAGAGAGCTTTCCTCGAGACTCTTGATGGATCATGCCGAACCCCGATTGCGGGATATGCGTCGAGGGATGAAGATGGCAATTGTATATTCAAAGGGTTGGTAGCTTCCCCGGACGGAACCCGAGGTACGATCTCAAACGTTCATGTTCGAATGAAATAACTTCTACATTGCTAGAACATTGAATATCTACATCTTTTACAGTTCTTGAAACTTCTCGACGAGGTTCGTACGCCATTGACGATATGATAGCAATGGGGAAGGATGCTGGCAAGGAGCTACTTTCTCGAGCAGGTCCGGGTTTTTTTGATAGCTAG

mRNA sequence

ATGAGAATCCTATCTGCCAACGCGAATGCAGAGATTTGGCCTGTTGGGTCATCAAAAACAATCCCTTCTAAGCTAACCCATCTCCTTTTTCTTTCTTCTTCTTCTTCGTCTTCTCAAATGGTTGCTTTATCTTCCCCATCTGTAAGCCACTCTCCCATGGCTCACCCTTGTAATCTTGCCTCCCTTTCTCTTCCTGGGTTCTCCTCACTTTCCCTTAAACCTCCGGCTTTCACTAATGGCCCCAAGAAACTTCATGGGTTTGGCCTCATAAGAGCCTCTGTTGCTGTGGAGCAGCAGGCAGGGAAGACCAAGGTTGCTCTCCTCAGAATTGGCACCAGAGGAAGCCCATTAGCACTTGCCCAGGCTCATGAGACAAGAGACAAGCTCATGGCTTCCCATCCTGAGCTAGCTGAAGATGGTGCCATTCAGATTGTTGTTATCAAAACCACTGGTGACAAAATACTCTCTCAGCCACTTGCAGACATTGGTGGGAAAGGCTTATTTACAAAAGAGATTGATGATGCACTTATCAATGGTGACATAGACATTGCTGTTCACTCAATGAAAGATGTCCCAACTTACTTACCTGAGAAAACCATCCTCCCGTGTAACCTTCCGAGGGAGGACGTCCGGGACGCATTCATTTCTCTACGTGCAGGTTCACTTGCCGAGCTTCCAGCTGGAAGCGTCGTCGGTACAGCTTCACTGAGAAGAAAGTCTCAGCTACTGAATCGATATCCGTCCCTCGAAGTACTCGAAAATTTTCGAGGTAATGTCCAAACAAGGTTGAAAAAACTAAACAAAGGAGTAGTCCAAGCAACACTATTAGCTCTAGCTGGACTTCGACGTTTAAATATGACAGAAAACGTTACTTCGATCCTTTCAATCGATGAAATGCTTCCAGCTGTTGCTCAGGGAGCTATCGGTATCGCCTGTCGAAGCGACGATGACAAAATGGCTGAGTACTTAGCCTCATTAAACCATGAGGAAACAAGACTGGCTGTTGTTTGTGAGAGAGCTTTCCTCGAGACTCTTGATGGATCATGCCGAACCCCGATTGCGGGATATGCGTCGAGGGATGAAGATGGCAATTGTATATTCAAAGGGTTGGTAGCTTCCCCGGACGGAACCCGAGTTCTTGAAACTTCTCGACGAGGTTCGTACGCCATTGACGATATGATAGCAATGGGGAAGGATGCTGGCAAGGAGCTACTTTCTCGAGCAGGTCCGGGTTTTTTTGATAGCTAG

Coding sequence (CDS)

ATGAGAATCCTATCTGCCAACGCGAATGCAGAGATTTGGCCTGTTGGGTCATCAAAAACAATCCCTTCTAAGCTAACCCATCTCCTTTTTCTTTCTTCTTCTTCTTCGTCTTCTCAAATGGTTGCTTTATCTTCCCCATCTGTAAGCCACTCTCCCATGGCTCACCCTTGTAATCTTGCCTCCCTTTCTCTTCCTGGGTTCTCCTCACTTTCCCTTAAACCTCCGGCTTTCACTAATGGCCCCAAGAAACTTCATGGGTTTGGCCTCATAAGAGCCTCTGTTGCTGTGGAGCAGCAGGCAGGGAAGACCAAGGTTGCTCTCCTCAGAATTGGCACCAGAGGAAGCCCATTAGCACTTGCCCAGGCTCATGAGACAAGAGACAAGCTCATGGCTTCCCATCCTGAGCTAGCTGAAGATGGTGCCATTCAGATTGTTGTTATCAAAACCACTGGTGACAAAATACTCTCTCAGCCACTTGCAGACATTGGTGGGAAAGGCTTATTTACAAAAGAGATTGATGATGCACTTATCAATGGTGACATAGACATTGCTGTTCACTCAATGAAAGATGTCCCAACTTACTTACCTGAGAAAACCATCCTCCCGTGTAACCTTCCGAGGGAGGACGTCCGGGACGCATTCATTTCTCTACGTGCAGGTTCACTTGCCGAGCTTCCAGCTGGAAGCGTCGTCGGTACAGCTTCACTGAGAAGAAAGTCTCAGCTACTGAATCGATATCCGTCCCTCGAAGTACTCGAAAATTTTCGAGGTAATGTCCAAACAAGGTTGAAAAAACTAAACAAAGGAGTAGTCCAAGCAACACTATTAGCTCTAGCTGGACTTCGACGTTTAAATATGACAGAAAACGTTACTTCGATCCTTTCAATCGATGAAATGCTTCCAGCTGTTGCTCAGGGAGCTATCGGTATCGCCTGTCGAAGCGACGATGACAAAATGGCTGAGTACTTAGCCTCATTAAACCATGAGGAAACAAGACTGGCTGTTGTTTGTGAGAGAGCTTTCCTCGAGACTCTTGATGGATCATGCCGAACCCCGATTGCGGGATATGCGTCGAGGGATGAAGATGGCAATTGTATATTCAAAGGGTTGGTAGCTTCCCCGGACGGAACCCGAGTTCTTGAAACTTCTCGACGAGGTTCGTACGCCATTGACGATATGATAGCAATGGGGAAGGATGCTGGCAAGGAGCTACTTTCTCGAGCAGGTCCGGGTTTTTTTGATAGCTAG

Protein sequence

MRILSANANAEIWPVGSSKTIPSKLTHLLFLSSSSSSSQMVALSSPSVSHSPMAHPCNLASLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQAGKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNKGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS

Homology

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BLAST of Csor.00g187130 vs. ExPASy Swiss-Prot
Match: Q43082 (Porphobilinogen deaminase, chloroplastic OS=Pisum sativum OX=3888 GN=HEMC PE=1 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 2.5e-159
Identity = 291/355 (81.97%), Postives = 320/355 (90.14%), Query Frame = 0


Query: 63  SLPGFSSLSLKPPAFTNGPKKLHGFG--LIRASVAVEQQAGKTKVALLRIGTRGSPLALA 122
           S P   SLSL   +F     K   F    IRAS+AVEQQ  + K AL+RIGTRGSPLALA
Sbjct: 15  SAPSNPSLSLFTSSFRFSSFKTSPFSKCRIRASLAVEQQTQQNKTALIRIGTRGSPLALA 74

Query: 123 QAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGD 182
           QAHETRDKLMASH ELAE+GAIQIV+IKTTGDKILSQPLADIGGKGLFTKEID+ALINGD
Sbjct: 75  QAHETRDKLMASHTELAEEGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEIDEALINGD 134

Query: 183 IDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKS 242
           IDIAVHSMKDVPTYLPE+TILPCNLPREDVRDAFISL A SLA+LPAGSV+GTASLRRKS
Sbjct: 135 IDIAVHSMKDVPTYLPEETILPCNLPREDVRDAFISLSAASLADLPAGSVIGTASLRRKS 194

Query: 243 QLLNRYPSLEVLENFRGNVQTRLKKLNKGVVQATLLALAGLRRLNMTENVTSILSIDEML 302
           Q+L+RYPSL V +NFRGNVQTRL+KL++GVV+ATLLALAGL+RLNMTENVTS LSID+ML
Sbjct: 195 QILHRYPSLTVQDNFRGNVQTRLRKLSEGVVKATLLALAGLKRLNMTENVTSTLSIDDML 254

Query: 303 PAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRD 362
           PAVAQGAIGIACRS+DDKMAEYLASLNHEETRLA+ CERAFL TLDGSCRTPIAGYASRD
Sbjct: 255 PAVAQGAIGIACRSNDDKMAEYLASLNHEETRLAISCERAFLTTLDGSCRTPIAGYASRD 314

Query: 363 EDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS 416
           +DGNC+F+GLVASPDGTRVLETSR GSY  +DM+ +GKDAG+ELLSRAGPGFF+S
Sbjct: 315 KDGNCLFRGLVASPDGTRVLETSRIGSYTYEDMMKIGKDAGEELLSRAGPGFFNS 369

[Close]

BLAST of Csor.00g187130 vs. ExPASy Swiss-Prot
Match: Q43316 (Porphobilinogen deaminase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HEMC PE=1 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 1.4e-154
Identity = 287/382 (75.13%), Postives = 321/382 (84.03%), Query Frame = 0


Query: 32  SSSSSSSQMVALSSPSVSHSPMAHPCNLASLSLPGFSSLSLKPPAFTNGPKKLHGFGLIR 91
           SSS S +  V L+      S   + C+L S+S  GFS   +  PA     +K    G ++
Sbjct: 5   SSSLSQAHKVVLTR---QPSSRVNTCSLGSVSAIGFSLPQISSPALGKCRRKQSSSGFVK 64

Query: 92  ASVAVEQQAGKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTG 151
           A VAVEQ   KT+ A++RIGTRGSPLALAQA+ETR+KL   HPEL EDGAI I +IKTTG
Sbjct: 65  ACVAVEQ---KTRTAIIRIGTRGSPLALAQAYETREKLKKKHPELVEDGAIHIEIIKTTG 124

Query: 152 DKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVR 211
           DKILSQPLADIGGKGLFTKEID+ALING IDIAVHSMKDVPTYLPEKTILPCNLPREDVR
Sbjct: 125 DKILSQPLADIGGKGLFTKEIDEALINGHIDIAVHSMKDVPTYLPEKTILPCNLPREDVR 184

Query: 212 DAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNKGVV 271
           DAFI L A +LAELPAGSVVGTASLRRKSQ+L++YP+L V ENFRGNVQTRL KL  G V
Sbjct: 185 DAFICLTAATLAELPAGSVVGTASLRRKSQILHKYPALHVEENFRGNVQTRLSKLQGGKV 244

Query: 272 QATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEET 331
           QATLLALAGL+RL+MTENV SILS+DEMLPAVAQGAIGIACR+DDDKMA YLASLNHEET
Sbjct: 245 QATLLALAGLKRLSMTENVASILSLDEMLPAVAQGAIGIACRTDDDKMATYLASLNHEET 304

Query: 332 RLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAID 391
           RLA+ CERAFLETLDGSCRTPIAGYAS+DE+GNCIF+GLVASPDGT+VLETSR+G Y  +
Sbjct: 305 RLAISCERAFLETLDGSCRTPIAGYASKDEEGNCIFRGLVASPDGTKVLETSRKGPYVYE 364

Query: 392 DMIAMGKDAGKELLSRAGPGFF 414
           DM+ MGKDAG+ELLSRAGPGFF
Sbjct: 365 DMVKMGKDAGQELLSRAGPGFF 380

[Close]

BLAST of Csor.00g187130 vs. ExPASy Swiss-Prot
Match: Q6H6D2 (Porphobilinogen deaminase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=HEMC PE=2 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 1.9e-138
Identity = 250/326 (76.69%), Postives = 285/326 (87.42%), Query Frame = 0


Query: 90  IRASVAVEQQAGKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKT 149
           +RA+VAV+ +A + KV+L+RIGTRGSPLALAQAHETRDKL A+H ELAE+GA++IV+IKT
Sbjct: 33  VRAAVAVQAEA-QAKVSLIRIGTRGSPLALAQAHETRDKLKAAHSELAEEGAVEIVIIKT 92

Query: 150 TGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPRED 209
           TGD IL +PLADIGGKGLFTKEIDDAL+ G IDIAVHSMKDVPTYLPE TILPCNLPRED
Sbjct: 93  TGDMILDKPLADIGGKGLFTKEIDDALLQGRIDIAVHSMKDVPTYLPEGTILPCNLPRED 152

Query: 210 VRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNKG 269
           VRDAFI L A SLAELPAGSVVG+ASLRR+SQ+L +YPSL+V+ NFRGNVQTRL+KL +G
Sbjct: 153 VRDAFICLTASSLAELPAGSVVGSASLRRQSQILYKYPSLKVV-NFRGNVQTRLRKLKEG 212

Query: 270 VVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHE 329
            V ATLLALAGL+RLNM E  TS+LS+DEMLPAVAQGAIGIACRS DD M  YL+SLNHE
Sbjct: 213 DVHATLLALAGLKRLNMAETATSVLSVDEMLPAVAQGAIGIACRSSDDTMMNYLSSLNHE 272

Query: 330 ETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYA 389
           +TRLAV CER FL  LDG+CRTPIA YASRD+DGNC F+GL+ASPDG+ V ETSR G Y 
Sbjct: 273 DTRLAVACEREFLSVLDGNCRTPIAAYASRDKDGNCSFRGLLASPDGSTVYETSRTGPYD 332

Query: 390 IDDMIAMGKDAGKELLSRAGPGFFDS 416
            D M+ MGKDAG EL ++AGPGFFDS
Sbjct: 333 FDIMVEMGKDAGHELKAKAGPGFFDS 356

[Close]

BLAST of Csor.00g187130 vs. ExPASy Swiss-Prot
Match: A1B853 (Porphobilinogen deaminase OS=Paracoccus denitrificans (strain Pd 1222) OX=318586 GN=hemC PE=3 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 7.0e-85
Identity = 175/307 (57.00%), Postives = 219/307 (71.34%), Query Frame = 0


Query: 108 LRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGL 167
           +RIGTRGS LALAQAHETRD+LMA+H  LA D A +IVVIKTTGD++L +PL +IGGKGL
Sbjct: 11  IRIGTRGSALALAQAHETRDRLMAAH-GLAAD-AFRIVVIKTTGDRVLDRPLKEIGGKGL 70

Query: 168 FTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRAGSLAELPA 227
           FT+EI+DAL+  +IDIAVHSMKD+PT  PE  ++ C LPREDVRDAF+S +  +++ELP 
Sbjct: 71  FTREIEDALLAHEIDIAVHSMKDMPTIQPEGLVIDCYLPREDVRDAFVSAQFAAISELPQ 130

Query: 228 GSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNKGVVQATLLALAGLRRLNMT 287
           G+VVG++SLRR++QL  R P L+++E FRGNVQTRLKKL  GV  AT LA+AGL RL M 
Sbjct: 131 GAVVGSSSLRRRAQLAARRPDLKLVE-FRGNVQTRLKKLEDGVAVATFLAMAGLTRLGML 190

Query: 288 ENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCERAFLETLDG 347
                 +  DEMLPAVAQG IG+  R+DD + A  LA+++  ++ L V  ERAFL  LDG
Sbjct: 191 HVARGAVEPDEMLPAVAQGCIGVERRADDARTASLLAAISDRDSALRVTAERAFLARLDG 250

Query: 348 SCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKDAGKELLSR 407
           SC+TPIAG A    D     +G +  PDG+ V+   R G  A  D  AMG D  +EL  R
Sbjct: 251 SCQTPIAGLAELQGD-RLRLRGEILRPDGSEVIAAERVGPAA--DGAAMGTDLAEELRGR 310

Query: 408 AGPGFFD 415
           A   FFD
Sbjct: 311 APADFFD 311

[Close]

BLAST of Csor.00g187130 vs. ExPASy Swiss-Prot
Match: Q1QQP5 (Porphobilinogen deaminase OS=Nitrobacter hamburgensis (strain DSM 10229 / NCIMB 13809 / X14) OX=323097 GN=hemC PE=3 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 2.0e-84
Identity = 176/316 (55.70%), Postives = 219/316 (69.30%), Query Frame = 0


Query: 98  QQAGKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQ 157
           Q +G+T + L  IGTRGS LALAQA+E RD+L  +H    E   I I  I+T+GD I  +
Sbjct: 2   QSSGETDI-LATIGTRGSALALAQANEVRDRLARAHQVAPE--RIVIKTIRTSGDAIQDR 61

Query: 158 PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 217
           PL D+GGKGLFTKEI++AL+ G ID AVHS KDVPT+LP+ T LP  LPREDVRDAFIS 
Sbjct: 62  PLFDVGGKGLFTKEIEEALLAGSIDFAVHSSKDVPTFLPDATWLPAFLPREDVRDAFISP 121

Query: 218 RAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNKGVVQATLLA 277
           RA SL +LPAGS+VGTASLRR++ +L   P L+V    RGNV+TRL+KL  G   ATLLA
Sbjct: 122 RAASLNDLPAGSIVGTASLRRQAMVLRLRPDLKV-SVIRGNVETRLRKLVAGEADATLLA 181

Query: 278 LAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVC 337
           LAGL RL + +  T IL  DE LPAV QGAI I  R DDD++  ++ ++   ET +A+  
Sbjct: 182 LAGLNRLGLQDRATRILETDEFLPAVGQGAIAIESRRDDDRINAFVKAIGDSETEVALSA 241

Query: 338 ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMG 397
           ER+FL  LDGSCRTPI G+   + D    F+GL+ SPDGT+  ET+R G+ A  D  A+G
Sbjct: 242 ERSFLALLDGSCRTPIGGHCRVNGD-RIHFRGLIISPDGTQSYETTREGARA--DAAALG 301

Query: 398 KDAGKELLSRAGPGFF 414
            DA +EL  RAG  FF
Sbjct: 302 ADAARELRERAGEKFF 310

[Close]

BLAST of Csor.00g187130 vs. NCBI nr
Match: KAG6581556.1 (Porphobilinogen deaminase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 797 bits (2059), Expect = 1.53e-290
Identity = 415/415 (100.00%), Postives = 415/415 (100.00%), Query Frame = 0


Query: 1   MRILSANANAEIWPVGSSKTIPSKLTHLLFLSSSSSSSQMVALSSPSVSHSPMAHPCNLA 60
           MRILSANANAEIWPVGSSKTIPSKLTHLLFLSSSSSSSQMVALSSPSVSHSPMAHPCNLA
Sbjct: 1   MRILSANANAEIWPVGSSKTIPSKLTHLLFLSSSSSSSQMVALSSPSVSHSPMAHPCNLA 60

Query: 61  SLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQAGKTKVALLRIGTRGSPLALA 120
           SLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQAGKTKVALLRIGTRGSPLALA
Sbjct: 61  SLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQAGKTKVALLRIGTRGSPLALA 120

Query: 121 QAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGD 180
           QAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGD
Sbjct: 121 QAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGD 180

Query: 181 IDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKS 240
           IDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKS
Sbjct: 181 IDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKS 240

Query: 241 QLLNRYPSLEVLENFRGNVQTRLKKLNKGVVQATLLALAGLRRLNMTENVTSILSIDEML 300
           QLLNRYPSLEVLENFRGNVQTRLKKLNKGVVQATLLALAGLRRLNMTENVTSILSIDEML
Sbjct: 241 QLLNRYPSLEVLENFRGNVQTRLKKLNKGVVQATLLALAGLRRLNMTENVTSILSIDEML 300

Query: 301 PAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRD 360
           PAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRD
Sbjct: 301 PAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRD 360

Query: 361 EDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS 415
           EDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS
Sbjct: 361 EDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS 415

[Close]

BLAST of Csor.00g187130 vs. NCBI nr
Match: XP_022934624.1 (porphobilinogen deaminase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 726 bits (1875), Expect = 3.86e-263
Identity = 375/376 (99.73%), Postives = 376/376 (100.00%), Query Frame = 0


Query: 40  MVALSSPSVSHSPMAHPCNLASLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQ 99
           MVALSSPSVSHSPMAHPCNLASLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQ
Sbjct: 1   MVALSSPSVSHSPMAHPCNLASLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQ 60

Query: 100 AGKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 159
           AGKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL
Sbjct: 61  AGKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120

Query: 160 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 219
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 180

Query: 220 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNKGVVQATLLALA 279
           GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLN+GVVQATLLALA
Sbjct: 181 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA 240

Query: 280 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCER 339
           GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCER 300

Query: 340 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 399
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 360

Query: 400 AGKELLSRAGPGFFDS 415
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of Csor.00g187130 vs. NCBI nr
Match: XP_023529085.1 (porphobilinogen deaminase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 716 bits (1849), Expect = 3.53e-259
Identity = 371/376 (98.67%), Postives = 372/376 (98.94%), Query Frame = 0


Query: 40  MVALSSPSVSHSPMAHPCNLASLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQ 99
           MVALSSPSVSHSPMA PCNLASLSLPGFSSLSLKPPAF N PKKLHG GLIRASVAVEQQ
Sbjct: 1   MVALSSPSVSHSPMARPCNLASLSLPGFSSLSLKPPAFINAPKKLHGLGLIRASVAVEQQ 60

Query: 100 AGKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 159
           AGKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL
Sbjct: 61  AGKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120

Query: 160 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 219
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 180

Query: 220 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNKGVVQATLLALA 279
           GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLN+GVVQATLLALA
Sbjct: 181 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA 240

Query: 280 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCER 339
           GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCER 300

Query: 340 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 399
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 360

Query: 400 AGKELLSRAGPGFFDS 415
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of Csor.00g187130 vs. NCBI nr
Match: XP_022982636.1 (porphobilinogen deaminase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 710 bits (1832), Expect = 1.38e-256
Identity = 368/376 (97.87%), Postives = 370/376 (98.40%), Query Frame = 0


Query: 40  MVALSSPSVSHSPMAHPCNLASLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQ 99
           MVALSSPSVS+SPMA PCNLASLSLPGFSSLSLKPPAFTN PKK HGFGLIRASVAVEQQ
Sbjct: 1   MVALSSPSVSNSPMARPCNLASLSLPGFSSLSLKPPAFTNAPKKFHGFGLIRASVAVEQQ 60

Query: 100 AGKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 159
             KTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL
Sbjct: 61  PEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120

Query: 160 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 219
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 180

Query: 220 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNKGVVQATLLALA 279
           GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLN+GVVQATLLALA
Sbjct: 181 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA 240

Query: 280 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCER 339
           GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMA YLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 300

Query: 340 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 399
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 360

Query: 400 AGKELLSRAGPGFFDS 415
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of Csor.00g187130 vs. NCBI nr
Match: KGN58748.2 (hypothetical protein Csa_002701 [Cucumis sativus])

HSP 1 Score: 708 bits (1828), Expect = 4.37e-255
Identity = 377/432 (87.27%), Postives = 388/432 (89.81%), Query Frame = 0


Query: 1   MRILSANANAEIWPVGSSKTIPSKLTHLLFLSS-----------------SSSSSQMVAL 60
           MRILSANANA+I PVGSSKTIPSKLTH  FL S                   + SQM  L
Sbjct: 1   MRILSANANADICPVGSSKTIPSKLTHFPFLLSFFLLLPLLLLLLISLNLKQTLSQMGVL 60

Query: 61  SSPSVSHSPMAHPCNLASLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQAGKT 120
           SSPSVSHSPM  PCN+ SLS  GFSSLSLKPP F+NG KK HG GLIRA VA EQQ  KT
Sbjct: 61  SSPSVSHSPMPRPCNVGSLSFLGFSSLSLKPPTFSNGAKKFHGVGLIRA-VAAEQQVEKT 120

Query: 121 KVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIG 180
           KVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIG
Sbjct: 121 KVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIG 180

Query: 181 GKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRAGSLA 240
           GKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL AGS A
Sbjct: 181 GKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSFA 240

Query: 241 ELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNKGVVQATLLALAGLRR 300
           ELPAGS++GTASLRRKSQLLNRYPSL+VLENFRGNVQTRL+KLN+GVVQATLLALAGLRR
Sbjct: 241 ELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRR 300

Query: 301 LNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCERAFLE 360
           LNMTENVTSILSIDEMLPAVAQGAIGIACRSDDD MA YLASLNHEETRLAVVCERAFLE
Sbjct: 301 LNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLE 360

Query: 361 TLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKDAGKE 415
           TLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRG YAI+DMIAMGKDAG+E
Sbjct: 361 TLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDAGQE 420

[Close]

BLAST of Csor.00g187130 vs. ExPASy TrEMBL
Match: A0A6J1F354 (Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111441758 PE=3 SV=1)

HSP 1 Score: 726 bits (1875), Expect = 1.87e-263
Identity = 375/376 (99.73%), Postives = 376/376 (100.00%), Query Frame = 0


Query: 40  MVALSSPSVSHSPMAHPCNLASLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQ 99
           MVALSSPSVSHSPMAHPCNLASLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQ
Sbjct: 1   MVALSSPSVSHSPMAHPCNLASLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQ 60

Query: 100 AGKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 159
           AGKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL
Sbjct: 61  AGKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120

Query: 160 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 219
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 180

Query: 220 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNKGVVQATLLALA 279
           GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLN+GVVQATLLALA
Sbjct: 181 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA 240

Query: 280 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCER 339
           GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCER 300

Query: 340 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 399
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 360

Query: 400 AGKELLSRAGPGFFDS 415
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of Csor.00g187130 vs. ExPASy TrEMBL
Match: A0A6J1IZW2 (Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111481450 PE=3 SV=1)

HSP 1 Score: 710 bits (1832), Expect = 6.66e-257
Identity = 368/376 (97.87%), Postives = 370/376 (98.40%), Query Frame = 0


Query: 40  MVALSSPSVSHSPMAHPCNLASLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQ 99
           MVALSSPSVS+SPMA PCNLASLSLPGFSSLSLKPPAFTN PKK HGFGLIRASVAVEQQ
Sbjct: 1   MVALSSPSVSNSPMARPCNLASLSLPGFSSLSLKPPAFTNAPKKFHGFGLIRASVAVEQQ 60

Query: 100 AGKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 159
             KTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL
Sbjct: 61  PEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120

Query: 160 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 219
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 180

Query: 220 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNKGVVQATLLALA 279
           GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLN+GVVQATLLALA
Sbjct: 181 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA 240

Query: 280 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCER 339
           GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMA YLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 300

Query: 340 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 399
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 360

Query: 400 AGKELLSRAGPGFFDS 415
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of Csor.00g187130 vs. ExPASy TrEMBL
Match: A0A6J1GCC7 (Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111452689 PE=3 SV=1)

HSP 1 Score: 677 bits (1746), Expect = 8.41e-244
Identity = 349/376 (92.82%), Postives = 357/376 (94.95%), Query Frame = 0


Query: 40  MVALSSPSVSHSPMAHPCNLASLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQ 99
           M   SSPS+SH PMA PCNL SLS  GFSSLSLKPPAF+ G KK HGFGLIRASVAVEQQ
Sbjct: 1   MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGGKKFHGFGLIRASVAVEQQ 60

Query: 100 AGKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 159
           A KTKVAL+RIGTRGSPLALAQAHETR+KLMA HPELAEDGAIQIV+IKTTGDKILSQPL
Sbjct: 61  AEKTKVALVRIGTRGSPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120

Query: 160 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 219
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL  
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSV 180

Query: 220 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNKGVVQATLLALA 279
           GSLAELPAGSV+GTASLRRKSQLLNRYPSL VLENFRGNVQTRL+KLN+GVVQATLLALA
Sbjct: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 280 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCER 339
           GLRRLNMTE VTSILSIDEMLPAVAQGAIGIACRSDDDKMA YLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 300

Query: 340 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 399
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRG YAIDDMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKD 360

Query: 400 AGKELLSRAGPGFFDS 415
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of Csor.00g187130 vs. ExPASy TrEMBL
Match: A0A6J1KA97 (Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111493083 PE=3 SV=1)

HSP 1 Score: 673 bits (1736), Expect = 2.80e-242
Identity = 346/376 (92.02%), Postives = 356/376 (94.68%), Query Frame = 0


Query: 40  MVALSSPSVSHSPMAHPCNLASLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQ 99
           M   SSPS+SH PMA PCNL SLS  GFSSLSLKPPAF+ G K+ HG GLIRASVAVEQQ
Sbjct: 1   MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGDKRFHGVGLIRASVAVEQQ 60

Query: 100 AGKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 159
           A KTKVAL+RIGTRGSPLALAQAHETR+KLMA HPELAEDGAIQIV+IKTTGDKILSQPL
Sbjct: 61  AEKTKVALVRIGTRGSPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120

Query: 160 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 219
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL  
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSV 180

Query: 220 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNKGVVQATLLALA 279
           GSLAELPAGSV+GTASLRRKSQLLNRYPSL VLENFRGNVQTRL+KLN+GVVQATLLALA
Sbjct: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 280 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCER 339
           GLRRLNMTE VTSILSIDEMLPAVAQGAIGIACRSDDDKMA YLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 300

Query: 340 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 399
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRG YAIDDM+AMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMVAMGKD 360

Query: 400 AGKELLSRAGPGFFDS 415
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of Csor.00g187130 vs. ExPASy TrEMBL
Match: A0A6J1DII9 (Hydroxymethylbilane synthase OS=Momordica charantia OX=3673 GN=LOC111020357 PE=3 SV=1)

HSP 1 Score: 663 bits (1710), Expect = 2.56e-238
Identity = 339/376 (90.16%), Postives = 356/376 (94.68%), Query Frame = 0


Query: 40  MVALSSPSVSHSPMAHPCNLASLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQ 99
           M   SSP ++HSPMA PCN+ SLS  GFSSLSLKPPAF NG KK  G GL+RASVAVEQQ
Sbjct: 1   MGVFSSPPITHSPMARPCNVGSLSPLGFSSLSLKPPAFCNGGKKFGGLGLVRASVAVEQQ 60

Query: 100 AGKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 159
             KTKVAL+RIGTRGSPLALAQA+ETRDKLMA HPELAEDGAIQI++IKTTGDKILSQPL
Sbjct: 61  TEKTKVALVRIGTRGSPLALAQAYETRDKLMAIHPELAEDGAIQIIIIKTTGDKILSQPL 120

Query: 160 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 219
           ADIGGKGLFTKEIDDALIN DIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL A
Sbjct: 121 ADIGGKGLFTKEIDDALINSDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180

Query: 220 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNKGVVQATLLALA 279
           GSLAELPAGSV+GTASLRRKSQLLNRYPSL+VLENFRGNVQTRL+KLN+GVVQATLLALA
Sbjct: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 280 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCER 339
           GLRRLNMTENVTS+LSIDEMLPAVAQGAIGIACRS+DDKMA YLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSVLSIDEMLPAVAQGAIGIACRSNDDKMANYLASLNHEETRLAVVCER 300

Query: 340 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 399
           AFLE LDGSCRTPIAGYAS+DEDGNCIFKGLVASPDGTRVLETSRRG+YAIDDM+AMGKD
Sbjct: 301 AFLEMLDGSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRRGAYAIDDMVAMGKD 360

Query: 400 AGKELLSRAGPGFFDS 415
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of Csor.00g187130 vs. TAIR 10
Match: AT5G08280.1 (hydroxymethylbilane synthase )

HSP 1 Score: 547.4 bits (1409), Expect = 1.0e-155
Identity = 287/382 (75.13%), Postives = 321/382 (84.03%), Query Frame = 0


Query: 32  SSSSSSSQMVALSSPSVSHSPMAHPCNLASLSLPGFSSLSLKPPAFTNGPKKLHGFGLIR 91
           SSS S +  V L+      S   + C+L S+S  GFS   +  PA     +K    G ++
Sbjct: 5   SSSLSQAHKVVLTR---QPSSRVNTCSLGSVSAIGFSLPQISSPALGKCRRKQSSSGFVK 64

Query: 92  ASVAVEQQAGKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTG 151
           A VAVEQ   KT+ A++RIGTRGSPLALAQA+ETR+KL   HPEL EDGAI I +IKTTG
Sbjct: 65  ACVAVEQ---KTRTAIIRIGTRGSPLALAQAYETREKLKKKHPELVEDGAIHIEIIKTTG 124

Query: 152 DKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVR 211
           DKILSQPLADIGGKGLFTKEID+ALING IDIAVHSMKDVPTYLPEKTILPCNLPREDVR
Sbjct: 125 DKILSQPLADIGGKGLFTKEIDEALINGHIDIAVHSMKDVPTYLPEKTILPCNLPREDVR 184

Query: 212 DAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNKGVV 271
           DAFI L A +LAELPAGSVVGTASLRRKSQ+L++YP+L V ENFRGNVQTRL KL  G V
Sbjct: 185 DAFICLTAATLAELPAGSVVGTASLRRKSQILHKYPALHVEENFRGNVQTRLSKLQGGKV 244

Query: 272 QATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEET 331
           QATLLALAGL+RL+MTENV SILS+DEMLPAVAQGAIGIACR+DDDKMA YLASLNHEET
Sbjct: 245 QATLLALAGLKRLSMTENVASILSLDEMLPAVAQGAIGIACRTDDDKMATYLASLNHEET 304

Query: 332 RLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAID 391
           RLA+ CERAFLETLDGSCRTPIAGYAS+DE+GNCIF+GLVASPDGT+VLETSR+G Y  +
Sbjct: 305 RLAISCERAFLETLDGSCRTPIAGYASKDEEGNCIFRGLVASPDGTKVLETSRKGPYVYE 364

Query: 392 DMIAMGKDAGKELLSRAGPGFF 414
           DM+ MGKDAG+ELLSRAGPGFF
Sbjct: 365 DMVKMGKDAGQELLSRAGPGFF 380

The following BLAST results are available for this feature:

BLAST of Csor.00g187130 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q43082	2.5e-159	81.97	Porphobilinogen deaminase, chloroplastic OS=Pisum sativum OX=3888 GN=HEMC PE=1 S...	[more]
Q43316	1.4e-154	75.13	Porphobilinogen deaminase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HEMC...	[more]
Q6H6D2	1.9e-138	76.69	Porphobilinogen deaminase, chloroplastic OS=Oryza sativa subsp. japonica OX=3994...	[more]
A1B853	7.0e-85	57.00	Porphobilinogen deaminase OS=Paracoccus denitrificans (strain Pd 1222) OX=318586...	[more]
Q1QQP5	2.0e-84	55.70	Porphobilinogen deaminase OS=Nitrobacter hamburgensis (strain DSM 10229 / NCIMB ...	[more]

BLAST of Csor.00g187130 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6581556.1	1.53e-290	100.00	Porphobilinogen deaminase, chloroplastic, partial [Cucurbita argyrosperma subsp....	[more]
XP_022934624.1	3.86e-263	99.73	porphobilinogen deaminase, chloroplastic-like [Cucurbita moschata]	[more]
XP_023529085.1	3.53e-259	98.67	porphobilinogen deaminase, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]
XP_022982636.1	1.38e-256	97.87	porphobilinogen deaminase, chloroplastic-like [Cucurbita maxima]	[more]
KGN58748.2	4.37e-255	87.27	hypothetical protein Csa_002701 [Cucumis sativus]	[more]

BLAST of Csor.00g187130 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F354	1.87e-263	99.73	Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111441758 PE=3 ...	[more]
A0A6J1IZW2	6.66e-257	97.87	Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111481450 PE=3 SV...	[more]
A0A6J1GCC7	8.41e-244	92.82	Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111452689 PE=3 ...	[more]
A0A6J1KA97	2.80e-242	92.02	Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111493083 PE=3 SV...	[more]
A0A6J1DII9	2.56e-238	90.16	Hydroxymethylbilane synthase OS=Momordica charantia OX=3673 GN=LOC111020357 PE=3...	[more]

BLAST of Csor.00g187130 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G08280.1	1.0e-155	75.13	hydroxymethylbilane synthase	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000860	Porphobilinogen deaminase	PRINTS	PR00151	PORPHBDMNASE	coord: 182..201 score: 61.19 coord: 151..171 score: 66.21 coord: 250..267 score: 52.38 coord: 337..354 score: 53.17 coord: 230..247 score: 57.41
IPR000860	Porphobilinogen deaminase	TIGRFAM	TIGR00212	TIGR00212	coord: 108..406 e-value: 5.1E-101 score: 335.5
IPR000860	Porphobilinogen deaminase	PANTHER	PTHR11557	PORPHOBILINOGEN DEAMINASE	coord: 51..414
IPR000860	Porphobilinogen deaminase	HAMAP	MF_00260	Porphobil_deam	coord: 107..407 score: 34.349174
IPR022418	Porphobilinogen deaminase, C-terminal	PFAM	PF03900	Porphobil_deamC	coord: 333..405 e-value: 2.7E-15 score: 56.5
IPR036803	Porphobilinogen deaminase, C-terminal domain superfamily	GENE3D	3.30.160.40		coord: 328..414 e-value: 4.9E-24 score: 86.5
IPR036803	Porphobilinogen deaminase, C-terminal domain superfamily	SUPERFAMILY	54782	Porphobilinogen deaminase (hydroxymethylbilane synthase), C-terminal domain	coord: 327..414
IPR022417	Porphobilinogen deaminase, N-terminal	PFAM	PF01379	Porphobil_deam	coord: 108..320 e-value: 2.2E-76 score: 256.0
None	No IPR available	GENE3D	3.40.190.10		coord: 209..309 e-value: 7.0E-82 score: 275.7
None	No IPR available	GENE3D	3.40.190.10		coord: 108..317 e-value: 7.0E-82 score: 275.7
None	No IPR available	PANTHER	PTHR11557:SF8	BNAC02G01240D PROTEIN	coord: 51..414
None	No IPR available	CDD	cd13648	PBP2_PBGD_1	coord: 107..386 e-value: 0.0 score: 513.883
None	No IPR available	SUPERFAMILY	53850	Periplasmic binding protein-like II	coord: 108..325
IPR022419	Porphobilinogen deaminase, dipyrromethane cofactor binding site	PROSITE	PS00533	PORPHOBILINOGEN_DEAM	coord: 338..354

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g187130.m01	Csor.00g187130.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0018160	peptidyl-pyrromethane cofactor linkage
biological_process	GO:0006782	protoporphyrinogen IX biosynthetic process
biological_process	GO:0050790	regulation of catalytic activity
biological_process	GO:0017157	regulation of exocytosis
biological_process	GO:0033014	tetrapyrrole biosynthetic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0005096	GTPase activator activity
molecular_function	GO:0004418	hydroxymethylbilane synthase activity
molecular_function	GO:0045159	myosin II binding
molecular_function	GO:0019905	syntaxin binding