Csor.00g187110 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g187110
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	acyl-CoA--sterol O-acyltransferase 1-like
Location	Csor_Chr14: 6415102 .. 6416166 (-)
RNA-Seq Expression	Csor.00g187110
Synteny	Csor.00g187110

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSsinglepolypeptidestart_codonstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGCCGAGATTCATGCATTCATTCAAACTCTGTTCCTGATTGCCTCTTCTCTCTGCCTCTGTTTCTCAATTAGAAACTCAATCCCAAATGGGTTCCTCAAATTCCTCTTTGTTCTTCCCTTCTTCTTCCTCTTCCTCTACATTCCTCTCCAATTCCAAACCATCCATCTCCGCGGCACCCTCGGCTTCTTCATCGGCTGGCTCGGCAGCTTCAAGCTCCTCCTCTTTGCCTTCGGCAAAGGCCCTCTCTGTTTTGCCGCCACCTCCTCCTCCCTCCGCCGCTTCCTCGCCGTCGCCTGCCTCCCAATCGACATCCCCGAACACAAATCTCCGCCCAACCGCTCCGATCCCGATCCGCTTCTCCCTCTGAGCTTCGCCGCAAAATTAGGGTTCCTAATCCTCACATTCGCCGCGATCTATTTCAAAAATCGATTCTACCCAAATGTGGTTCTAATTTTCTTCTGCCTCCTAATTTACCTCCTCCTCGAAATTCTCATCGGCGGAGCCACCGCCCTGGCGGAGGCGGTGCTCGGAGTCGAGTTATTGCCCTTTTTCGATGAGCCCTATCTCTCTGATTCGTTACAGGACTTTTGGGGGCGACGATGGAATCTAGTTTCCTCCAGAATCCTCCGCCCCGCGGTTTTCGATCCGTGCCGGAAACTCGCCGGCACTGTCGTCGGGAAGAAACCGGCGGCGATTATAGCGGTTATGGCGACATTTGCTGTCTCCGGTGTTATGCACGAACTGATCTACTTCTACATGGGAAGACTGACGCCGACTTGGGAGGTGAGTTGGTTCTTTCTGATACATGGAGTTTGTCTGGCGGCGGAGATGGCGGTGAAGTCAGCGGTGGGCGGGAGGTTCCGGCCGCCGCGGGTTGTATCTGGGGTGTTAACGATCGGGTTCGTGATGGCGACGAGTTTTTGGCTCTTTTTCCCGCAGCTTTTGAGGTTTAAAGCGGATGTCAGAATGCTTGAAGAGTACGCGGCTTTGGGCGCGTTTGTGAAGAGTGTGACGGCCGAGTTAATCGGACCGTTGTTCATTTCATTTCAAGTCAAATAA

mRNA sequence

ATGGCTGCCGAGATTCATGCATTCATTCAAACTCTGTTCCTGATTGCCTCTTCTCTCTGCCTCTGTTTCTCAATTAGAAACTCAATCCCAAATGGGTTCCTCAAATTCCTCTTTGTTCTTCCCTTCTTCTTCCTCTTCCTCTACATTCCTCTCCAATTCCAAACCATCCATCTCCGCGGCACCCTCGGCTTCTTCATCGGCTGGCTCGGCAGCTTCAAGCTCCTCCTCTTTGCCTTCGGCAAAGGCCCTCTCTGTTTTGCCGCCACCTCCTCCTCCCTCCGCCGCTTCCTCGCCGTCGCCTGCCTCCCAATCGACATCCCCGAACACAAATCTCCGCCCAACCGCTCCGATCCCGATCCGCTTCTCCCTCTGAGCTTCGCCGCAAAATTAGGGTTCCTAATCCTCACATTCGCCGCGATCTATTTCAAAAATCGATTCTACCCAAATGTGGTTCTAATTTTCTTCTGCCTCCTAATTTACCTCCTCCTCGAAATTCTCATCGGCGGAGCCACCGCCCTGGCGGAGGCGGTGCTCGGAGTCGAGTTATTGCCCTTTTTCGATGAGCCCTATCTCTCTGATTCGTTACAGGACTTTTGGGGGCGACGATGGAATCTAGTTTCCTCCAGAATCCTCCGCCCCGCGGTTTTCGATCCGTGCCGGAAACTCGCCGGCACTGTCGTCGGGAAGAAACCGGCGGCGATTATAGCGGTTATGGCGACATTTGCTGTCTCCGGTGTTATGCACGAACTGATCTACTTCTACATGGGAAGACTGACGCCGACTTGGGAGGTGAGTTGGTTCTTTCTGATACATGGAGTTTGTCTGGCGGCGGAGATGGCGGTGAAGTCAGCGGTGGGCGGGAGGTTCCGGCCGCCGCGGGTTGTATCTGGGGTGTTAACGATCGGGTTCGTGATGGCGACGAGTTTTTGGCTCTTTTTCCCGCAGCTTTTGAGGTTTAAAGCGGATGTCAGAATGCTTGAAGAGTACGCGGCTTTGGGCGCGTTTGTGAAGAGTGTGACGGCCGAGTTAATCGGACCGTTGTTCATTTCATTTCAAGTCAAATAA

Coding sequence (CDS)

ATGGCTGCCGAGATTCATGCATTCATTCAAACTCTGTTCCTGATTGCCTCTTCTCTCTGCCTCTGTTTCTCAATTAGAAACTCAATCCCAAATGGGTTCCTCAAATTCCTCTTTGTTCTTCCCTTCTTCTTCCTCTTCCTCTACATTCCTCTCCAATTCCAAACCATCCATCTCCGCGGCACCCTCGGCTTCTTCATCGGCTGGCTCGGCAGCTTCAAGCTCCTCCTCTTTGCCTTCGGCAAAGGCCCTCTCTGTTTTGCCGCCACCTCCTCCTCCCTCCGCCGCTTCCTCGCCGTCGCCTGCCTCCCAATCGACATCCCCGAACACAAATCTCCGCCCAACCGCTCCGATCCCGATCCGCTTCTCCCTCTGAGCTTCGCCGCAAAATTAGGGTTCCTAATCCTCACATTCGCCGCGATCTATTTCAAAAATCGATTCTACCCAAATGTGGTTCTAATTTTCTTCTGCCTCCTAATTTACCTCCTCCTCGAAATTCTCATCGGCGGAGCCACCGCCCTGGCGGAGGCGGTGCTCGGAGTCGAGTTATTGCCCTTTTTCGATGAGCCCTATCTCTCTGATTCGTTACAGGACTTTTGGGGGCGACGATGGAATCTAGTTTCCTCCAGAATCCTCCGCCCCGCGGTTTTCGATCCGTGCCGGAAACTCGCCGGCACTGTCGTCGGGAAGAAACCGGCGGCGATTATAGCGGTTATGGCGACATTTGCTGTCTCCGGTGTTATGCACGAACTGATCTACTTCTACATGGGAAGACTGACGCCGACTTGGGAGGTGAGTTGGTTCTTTCTGATACATGGAGTTTGTCTGGCGGCGGAGATGGCGGTGAAGTCAGCGGTGGGCGGGAGGTTCCGGCCGCCGCGGGTTGTATCTGGGGTGTTAACGATCGGGTTCGTGATGGCGACGAGTTTTTGGCTCTTTTTCCCGCAGCTTTTGAGGTTTAAAGCGGATGTCAGAATGCTTGAAGAGTACGCGGCTTTGGGCGCGTTTGTGAAGAGTGTGACGGCCGAGTTAATCGGACCGTTGTTCATTTCATTTCAAGTCAAATAA

Protein sequence

MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRGTLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDPLLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGVELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMATFAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVLTIGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIGPLFISFQVK

Homology

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BLAST of Csor.00g187110 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 8.4e-71
Identity = 148/344 (43.02%), Postives = 203/344 (59.01%), Query Frame = 0


Query: 7   AFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRGTLGFFI 66
           +FI+   L+  SLC  F I   +P G  + +   P F +F  +P    ++HL G   FFI
Sbjct: 3   SFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFI 62

Query: 67  GWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDPLL--PL 126
            WL +FKLLLFA G+GPL       SL  FLAV+CLPI I     P      +     PL
Sbjct: 63  AWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGPL 122

Query: 127 SFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGVELLP 186
            +  K  F++L   A  +  +    VVL  + + IY  LEI++    A   A+  +EL P
Sbjct: 123 IYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEP 182

Query: 187 FFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMATFAVS 246
            F++PYL+ SLQDFWGRRWNL+ + ILRP V++P  +L  +V+G   + I+A   TF VS
Sbjct: 183 QFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLF-SVLGPNYSQILAAFGTFVVS 242

Query: 247 GVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVLTIGFV 306
           G+MHELI+FYMGRL P W++ WFFLI+G C   E+A+K  + GR+R P+ +S VLT+ FV
Sbjct: 243 GIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLTFV 302

Query: 307 MATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIGPLF 349
           M T+ WLF P+  R     + L+EYAA+GAF   V  +L   LF
Sbjct: 303 MVTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEVRRKLTAYLF 345

[Close]

BLAST of Csor.00g187110 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 8.7e-60
Identity = 139/338 (41.12%), Postives = 194/338 (57.40%), Query Frame = 0


Query: 1   MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 60
           M  E+ + I+       S+  C+     I +G  + L VLP   LFL +PL   ++H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP 120
           +  FF+ WL +FKL+LF+F +GPL      S+L RF+   C PI + ++  P N+     
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPL--FPVPSNLSRFVCFTCFPIKLQQNPKPQNQ----- 120

Query: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGV 180
           +    FA KL F  +      +K    P V+L+ + L IYL  EIL+     L    L  
Sbjct: 121 MPKWGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWC 180

Query: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMAT 240
           +L P F+EPYLS SLQDFWGRRWNL+   ILRPAV+ P R++AG  +    A  + V A+
Sbjct: 181 DLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFAS 240

Query: 241 FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVK-SAVGGRFRPPRVVSGVL 300
           F VSGV+HELI+FY  R +PT EV+ FF++HGVC AAE A K + +  R++  ++VS +L
Sbjct: 241 FLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMVSRLL 300

Query: 301 TIGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVK 338
           T+GFV+ T  WLFFP L R     R+ +E      FVK
Sbjct: 301 TVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVK 331

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BLAST of Csor.00g187110 vs. ExPASy Swiss-Prot
Match: Q9XGY6 (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 4.8e-58
Identity = 133/348 (38.22%), Postives = 193/348 (55.46%), Query Frame = 0


Query: 4   EIHAFIQTLFLIASSLCLCFSIRNSIPN-GFLKFLFVLPFFFLFLYIPLQFQTIHLRGTL 63
           E+  F +      ++ C C  +    P+ G L+ L +LP   LF+++PL+  + HL G  
Sbjct: 6   ELKTFSEVWISAIAAACYCRFVPAVAPHGGALRLLLLLPVVLLFIFLPLRLSSFHLGGPT 65

Query: 64  GFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDPL- 123
             ++ WL +FKLLLFAF  GPL  +  S SL  F++   LPI   +  S  +  +   L 
Sbjct: 66  ALYLVWLANFKLLLFAFHLGPL--SNPSLSLLHFISTTLLPIKFRDDPSNDHEKNKRTLS 125

Query: 124 ----LPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAV 183
                 + F AKL F         F+      V+ + +C   YL  EI +  +  +A A 
Sbjct: 126 FEWRKVVLFVAKLVFFAGILKIYEFRKDLPHFVISVLYCFHFYLGTEITLAASAVIARAT 185

Query: 184 LGVELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAV 243
           LG++L P F+EPYL+ SLQDFWGRRWNL+ S IL    + P R++    V  +     A+
Sbjct: 186 LGLDLYPQFNEPYLATSLQDFWGRRWNLMVSDILGLTTYQPVRRVLSRWVRLRWEVAGAM 245

Query: 244 MATFAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSG 303
           +  F VSG+MHE+ +FY+ R  P+WEV+ FF++HGVC A EM VK AV G+ R  R VSG
Sbjct: 246 LVAFTVSGLMHEVFFFYLTRARPSWEVTGFFVLHGVCTAVEMVVKKAVSGKVRLRREVSG 305

Query: 304 VLTIGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIG 346
            LT+GFVM T  WLF PQL+R   D++ ++EY  +  + +     L+G
Sbjct: 306 ALTVGFVMVTGGWLFLPQLVRHGVDLKTIDEYPVMFNYTQKKLMGLLG 351

[Close]

BLAST of Csor.00g187110 vs. ExPASy Swiss-Prot
Match: Q9FJ74 (Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=AT3 PE=3 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 5.9e-56
Identity = 138/339 (40.71%), Postives = 190/339 (56.05%), Query Frame = 0


Query: 1   MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 60
           M  E+  FI+       S+  C+ +   I  G  + L VLP   LFL  PL F  +H  G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP 120
            + FF+ WL +FKL+LF+F +GPL  +    +L RF+ + C PI   ++ +  N   P  
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPL--SPLPRTLSRFICITCFPIKPQQNPNIQNYKIPIW 120

Query: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGV 180
           L    FA K+   ++      +K    P ++L+F  L I+L LEI+     AL    LG 
Sbjct: 121 L----FAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALVFITLGC 180

Query: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMAT 240
           +L P  +EPYL+ SLQDFWGRRWNL+   ILRPAV+ P R++A   V    A  + V A 
Sbjct: 181 DLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMFLGVFAA 240

Query: 241 FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGR--FRPPRVVSGV 300
           F VSG +HE+++FY+ R  PT EV+WFFL+HGVC  AE+AVK +   R  +R    VS +
Sbjct: 241 FLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVSPTVSRL 300

Query: 301 LTIGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVK 338
           LT+GFV+ TS W FFP L+R     R+  E      FVK
Sbjct: 301 LTVGFVVVTSGWFFFP-LIRSGIIERLASEALMCIDFVK 332

[Close]

BLAST of Csor.00g187110 vs. ExPASy Swiss-Prot
Match: Q9FJ73 (Probable long-chain-alcohol O-fatty-acyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=AT2 PE=3 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 2.1e-53
Identity = 133/336 (39.58%), Postives = 190/336 (56.55%), Query Frame = 0


Query: 1   MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 60
           M  E+   I+       S+  C+ I + I  G L+ L +LP F +FL +PL F ++H   
Sbjct: 1   MEEELRNLIKVWISALISISYCYYISSKISKGVLRLLSLLPIFIIFLLLPLFFSSVHFCV 60

Query: 61  TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP 120
             GFF  WL +FKL LFAF + PL  +   S+L RF   AC PI I ++ S  NR    P
Sbjct: 61  ISGFFFTWLANFKLFLFAFDQEPL--SPLPSNLTRFFCFACFPIKINKNPS-SNRIHNKP 120

Query: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNV----VLIFFCLLIYLLLEILIGGATALAEA 180
           +     A KL  LI +F    ++N +   +     L  F + +YL  E+++    AL   
Sbjct: 121 MSKWVLAFKL--LIFSFLLHVYRNNYDSGLSRFAFLALFTIHVYLEAELILVFVGALMSM 180

Query: 181 VLGVELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIA 240
           +LG E+ P F++PYL+ SLQ+FW RRWNL+   +LRPAV  P ++    ++G   A    
Sbjct: 181 LLGCEMEPVFNDPYLATSLQEFWSRRWNLMVPAVLRPAVHIPVQRFCAPLLGLHRAFYAG 240

Query: 241 VMATFAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFR----PP 300
           ++ATF VSG+MHELIYFY+ R +PTWEV+ FFL+HGV    E+A+K     R R    P 
Sbjct: 241 MLATFIVSGLMHELIYFYVIRKSPTWEVTCFFLLHGVVTCLEIAMK-----RMRWLPTPR 300

Query: 301 RVVSGVLTIGFVMATSFWLFFPQLLRFKADVRMLEE 329
           R VSG+    F++ T+ WLF+PQ+LR     R++ E
Sbjct: 301 RAVSGLAITVFLLVTAGWLFYPQMLRNDVHKRVISE 326

[Close]

BLAST of Csor.00g187110 vs. NCBI nr
Match: XP_022934181.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata] >KAG6581558.1 Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 687 bits (1774), Expect = 3.61e-249
Identity = 354/354 (100.00%), Postives = 354/354 (100.00%), Query Frame = 0


Query: 1   MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 60
           MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG
Sbjct: 1   MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 60

Query: 61  TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP 120
           TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP
Sbjct: 61  TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP 120

Query: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGV 180
           LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGV
Sbjct: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGV 180

Query: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMAT 240
           ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMAT
Sbjct: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMAT 240

Query: 241 FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVLT 300
           FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVLT
Sbjct: 241 FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVLT 300

Query: 301 IGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIGPLFISFQVK 354
           IGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIGPLFISFQVK
Sbjct: 301 IGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIGPLFISFQVK 354

[Close]

BLAST of Csor.00g187110 vs. NCBI nr
Match: KAG7018062.1 (Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 687 bits (1774), Expect = 3.56e-246
Identity = 354/354 (100.00%), Postives = 354/354 (100.00%), Query Frame = 0


Query: 1   MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 60
           MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG
Sbjct: 188 MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 247

Query: 61  TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP 120
           TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP
Sbjct: 248 TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP 307

Query: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGV 180
           LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGV
Sbjct: 308 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGV 367

Query: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMAT 240
           ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMAT
Sbjct: 368 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMAT 427

Query: 241 FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVLT 300
           FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVLT
Sbjct: 428 FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVLT 487

Query: 301 IGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIGPLFISFQVK 354
           IGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIGPLFISFQVK
Sbjct: 488 IGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIGPLFISFQVK 541

[Close]

BLAST of Csor.00g187110 vs. NCBI nr
Match: XP_022982637.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 664 bits (1712), Expect = 1.02e-239
Identity = 342/354 (96.61%), Postives = 347/354 (98.02%), Query Frame = 0


Query: 1   MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 60
           MAAEIHAFIQTLFLI+SSLCLCFSIRNSIPNGFLKF+FVLPFFFLFLYIPLQFQTIHLRG
Sbjct: 1   MAAEIHAFIQTLFLISSSLCLCFSIRNSIPNGFLKFIFVLPFFFLFLYIPLQFQTIHLRG 60

Query: 61  TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP 120
           TLGFFIGWLGSFKLLLFAFGKGPLC AATSSSLRRFLAVA LPIDIPEHK PPNRSDPDP
Sbjct: 61  TLGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVASLPIDIPEHKPPPNRSDPDP 120

Query: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGV 180
           LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATA+AEAVLGV
Sbjct: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATAVAEAVLGV 180

Query: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMAT 240
           ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPA IIAVMAT
Sbjct: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAVIIAVMAT 240

Query: 241 FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVLT 300
           FAVSGVMHELIYFYMGRL PTWEVSWFFLIHGVCLAAEMAVKSAVG RFR PRVVSGVLT
Sbjct: 241 FAVSGVMHELIYFYMGRLAPTWEVSWFFLIHGVCLAAEMAVKSAVGRRFRAPRVVSGVLT 300

Query: 301 IGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIGPLFISFQVK 354
           +GFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVK+VTAELIGPLFISFQVK
Sbjct: 301 MGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKNVTAELIGPLFISFQVK 354

[Close]

BLAST of Csor.00g187110 vs. NCBI nr
Match: XP_023528035.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 660 bits (1703), Expect = 2.39e-238
Identity = 340/354 (96.05%), Postives = 346/354 (97.74%), Query Frame = 0


Query: 1   MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 60
           MAAEIHAFIQTLFLI++SLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG
Sbjct: 1   MAAEIHAFIQTLFLISTSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 60

Query: 61  TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP 120
           TLGFFIGWLGSFKLLLFAFGKGPLC AATSSSLRRFLAVA LPIDIPEHK PP+RSDPDP
Sbjct: 61  TLGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVASLPIDIPEHKPPPDRSDPDP 120

Query: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGV 180
           LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLI FCLLIYLLLEILIGGATALAEAVLGV
Sbjct: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLILFCLLIYLLLEILIGGATALAEAVLGV 180

Query: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMAT 240
           E LPFFDEPYLSDSLQDFWGRRWNLVSSRILR AVFDPCRKLAGT VGKKPAAIIAVMAT
Sbjct: 181 EFLPFFDEPYLSDSLQDFWGRRWNLVSSRILRSAVFDPCRKLAGTFVGKKPAAIIAVMAT 240

Query: 241 FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVLT 300
           FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSG+LT
Sbjct: 241 FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGLLT 300

Query: 301 IGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIGPLFISFQVK 354
           IGFVMATSFWLFFPQLLRFKADVRMLEEYA+LGAFVKSVTAE+I PLFISFQVK
Sbjct: 301 IGFVMATSFWLFFPQLLRFKADVRMLEEYASLGAFVKSVTAEMIAPLFISFQVK 354

[Close]

BLAST of Csor.00g187110 vs. NCBI nr
Match: XP_038905685.1 (acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida])

HSP 1 Score: 541 bits (1394), Expect = 2.79e-191
Identity = 272/354 (76.84%), Postives = 309/354 (87.29%), Query Frame = 0


Query: 1   MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 60
           MAAEI  F+QTLFL++SSLC CF+IRNSIPNGFLKFL +LPFFFLFLYIPLQFQ+IH +G
Sbjct: 1   MAAEIRIFLQTLFLVSSSLCFCFTIRNSIPNGFLKFLLILPFFFLFLYIPLQFQSIHFQG 60

Query: 61  TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP 120
           ++GFFIGWLGSFKLLLFAFGKGPLC AA SSSLR FLAV  LPI+IP+HK   N S  +P
Sbjct: 61  SIGFFIGWLGSFKLLLFAFGKGPLCSAAASSSLRHFLAVGSLPIEIPDHKPAANHSHSNP 120

Query: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGV 180
           +LPLSF++KLG LILT  AIYFKN F+PN +LIFFC +IYLLLEIL+GGA ALA+A LGV
Sbjct: 121 VLPLSFSSKLGLLILTIVAIYFKNLFHPNAILIFFCFVIYLLLEILLGGAAALAKATLGV 180

Query: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMAT 240
           ELLP+FDEPYLSDSLQDFWGRRWNL++SRILR  V+DPCRK+   +VG+KPAA++A+ AT
Sbjct: 181 ELLPYFDEPYLSDSLQDFWGRRWNLMTSRILRLVVYDPCRKITIGIVGRKPAAMMAMTAT 240

Query: 241 FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVLT 300
           FAVSG+MHELIYFYMGRL PTWEV+ FFL+HGVCLAAEMA+KSAVGGRFR P VVS  LT
Sbjct: 241 FAVSGLMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMAIKSAVGGRFRLPGVVSAALT 300

Query: 301 IGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIGPLFISFQVK 354
           IGFVM TSFWLFFPQ L+ K+DVRM EEYAALGAFVKSVT +LI PL ISF  K
Sbjct: 301 IGFVMTTSFWLFFPQFLKLKSDVRMFEEYAALGAFVKSVTGQLITPLNISFHFK 354

[Close]

BLAST of Csor.00g187110 vs. ExPASy TrEMBL
Match: A0A6J1F144 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111441426 PE=3 SV=1)

HSP 1 Score: 687 bits (1774), Expect = 1.75e-249
Identity = 354/354 (100.00%), Postives = 354/354 (100.00%), Query Frame = 0


Query: 1   MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 60
           MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG
Sbjct: 1   MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 60

Query: 61  TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP 120
           TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP
Sbjct: 61  TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP 120

Query: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGV 180
           LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGV
Sbjct: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGV 180

Query: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMAT 240
           ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMAT
Sbjct: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMAT 240

Query: 241 FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVLT 300
           FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVLT
Sbjct: 241 FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVLT 300

Query: 301 IGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIGPLFISFQVK 354
           IGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIGPLFISFQVK
Sbjct: 301 IGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIGPLFISFQVK 354

[Close]

BLAST of Csor.00g187110 vs. ExPASy TrEMBL
Match: A0A6J1IX47 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111481451 PE=3 SV=1)

HSP 1 Score: 664 bits (1712), Expect = 4.92e-240
Identity = 342/354 (96.61%), Postives = 347/354 (98.02%), Query Frame = 0


Query: 1   MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 60
           MAAEIHAFIQTLFLI+SSLCLCFSIRNSIPNGFLKF+FVLPFFFLFLYIPLQFQTIHLRG
Sbjct: 1   MAAEIHAFIQTLFLISSSLCLCFSIRNSIPNGFLKFIFVLPFFFLFLYIPLQFQTIHLRG 60

Query: 61  TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP 120
           TLGFFIGWLGSFKLLLFAFGKGPLC AATSSSLRRFLAVA LPIDIPEHK PPNRSDPDP
Sbjct: 61  TLGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVASLPIDIPEHKPPPNRSDPDP 120

Query: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGV 180
           LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATA+AEAVLGV
Sbjct: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATAVAEAVLGV 180

Query: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMAT 240
           ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPA IIAVMAT
Sbjct: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAVIIAVMAT 240

Query: 241 FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVLT 300
           FAVSGVMHELIYFYMGRL PTWEVSWFFLIHGVCLAAEMAVKSAVG RFR PRVVSGVLT
Sbjct: 241 FAVSGVMHELIYFYMGRLAPTWEVSWFFLIHGVCLAAEMAVKSAVGRRFRAPRVVSGVLT 300

Query: 301 IGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIGPLFISFQVK 354
           +GFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVK+VTAELIGPLFISFQVK
Sbjct: 301 MGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKNVTAELIGPLFISFQVK 354

[Close]

BLAST of Csor.00g187110 vs. ExPASy TrEMBL
Match: A0A6J1GBB6 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111452615 PE=3 SV=1)

HSP 1 Score: 492 bits (1267), Expect = 2.96e-172
Identity = 256/355 (72.11%), Postives = 292/355 (82.25%), Query Frame = 0


Query: 1   MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 60
           MAAEI  F+QTL  ++SSLC CFSIRNSIPNGFLKFL +LPFF LFLYIPLQFQT+HL+G
Sbjct: 1   MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG 60

Query: 61  TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP 120
            +GFFIGWL SFKLLLFAFGKGPLC A  SSSLRRFL V  LPIDI +H S   R D + 
Sbjct: 61  AIGFFIGWLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHDSASTRPDTNS 120

Query: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGV 180
           +LPLS ++KL FL L+ AAIY KN F+PN  LI FC +IYLLLEI++GGA A+A+A LGV
Sbjct: 121 VLPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGV 180

Query: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMAT 240
           ELLP FDEPYLS SLQDFWGRRWNL+ SRILR +V+DPCR  A  +VG+KPAAI+A  AT
Sbjct: 181 ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTAT 240

Query: 241 FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVLT 300
           FAVSG+MHELIYFY+GRL PTWEV+ FFL+HG  LA EMAVK+AVG RFRPPR+V+  LT
Sbjct: 241 FAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLT 300

Query: 301 IGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIGPL-FISFQVK 354
           IGFVM TSFWLFFPQ  RFK DVRM EE+AA+GAFVK+V AELI  +  ISFQVK
Sbjct: 301 IGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKNVAAELITSVKLISFQVK 355

[Close]

BLAST of Csor.00g187110 vs. ExPASy TrEMBL
Match: A0A6J1KCL2 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493090 PE=3 SV=1)

HSP 1 Score: 489 bits (1258), Expect = 6.92e-171
Identity = 256/355 (72.11%), Postives = 290/355 (81.69%), Query Frame = 0


Query: 1   MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 60
           MAAEI  F+QTL  ++SSLC CFSIRNSIPNGFLKFL +LPFF LFLYIPLQFQT+HL+G
Sbjct: 1   MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG 60

Query: 61  TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP 120
            +GFFIGWL SFKLLLFAFGKG LC AA SSSL RFL V  LPIDI +H     R D   
Sbjct: 61  AIGFFIGWLASFKLLLFAFGKGSLCSAAASSSLSRFLLVGSLPIDIYDHDPDSTRPDTTS 120

Query: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGV 180
           +LPLS ++KL FLIL+ AAIY KN F+PN  LI FC +IYLLLEI++GGA A+A+A LGV
Sbjct: 121 VLPLSLSSKLFFLILSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGV 180

Query: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMAT 240
           ELLP FDEPYLS SLQDFWGRRWNL+ SRILR +V+DPCR  A  +VG+KPAAI+A+ AT
Sbjct: 181 ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMAMTAT 240

Query: 241 FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVLT 300
           FAVSG+MHELIYFYMGRL PTWEV+ FFL+HG  LA EMAVK+AVG RFRPPR+V+  LT
Sbjct: 241 FAVSGLMHELIYFYMGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLT 300

Query: 301 IGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIGPL-FISFQVK 354
           IGFVM TSFWLFFPQ  RFK DVRM EE+AALGAFVK+V  ELI  +  ISFQVK
Sbjct: 301 IGFVMVTSFWLFFPQFFRFKPDVRMFEEHAALGAFVKNVAGELITSVKLISFQVK 355

[Close]

BLAST of Csor.00g187110 vs. ExPASy TrEMBL
Match: A0A6J1DI64 (acyl-CoA--sterol O-acyltransferase 1-like OS=Momordica charantia OX=3673 GN=LOC111020278 PE=3 SV=1)

HSP 1 Score: 496 bits (1278), Expect = 1.02e-170
Identity = 252/349 (72.21%), Postives = 292/349 (83.67%), Query Frame = 0


Query: 1   MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 60
           MA EI  F+QT+ L+ SSLC CFSIRNSIP+GFL+FL +LPFFFLFL IPLQF TIHL+G
Sbjct: 219 MATEIGIFVQTVLLVCSSLCFCFSIRNSIPHGFLRFLLILPFFFLFLCIPLQFHTIHLQG 278

Query: 61  TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPD- 120
            +GFFIGWLGSFKLLLFAFGKGPLC A  ++SLRRFLAV  LPI+IP H  PP    PD 
Sbjct: 279 AIGFFIGWLGSFKLLLFAFGKGPLCSAPAAASLRRFLAVGSLPIEIPVH--PPQAPAPDS 338

Query: 121 -PLLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVL 180
            P+L L+F AKLG L+LTF AIYF+N F P  VL+F+CLLIYLLLEIL+GGA ALA  +L
Sbjct: 339 PPVLSLTFPAKLGLLLLTFCAIYFRNYFNPYAVLVFYCLLIYLLLEILVGGAAALARTLL 398

Query: 181 GVELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVM 240
           GV+L P+FDEPYLS SLQ+FWGRRWNL+++RILR AV+DPCR++AG  VGK+ A ++AV 
Sbjct: 399 GVDLSPYFDEPYLSASLQEFWGRRWNLMTTRILRVAVYDPCRRVAGAAVGKRAAVMLAVA 458

Query: 241 ATFAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGV 300
           ATFAVSG+MHELIYFYMGR+ PTWEV+ FFL+HGVCL AEMAVK+A GGRFR PR VS  
Sbjct: 459 ATFAVSGLMHELIYFYMGRMAPTWEVTCFFLLHGVCLPAEMAVKAAAGGRFRLPRAVSAP 518

Query: 301 LTIGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIGPL 347
           LT+GFVM TSFWLFFPQ LRFKADVRM EEYAALGA VK+VT ++I PL
Sbjct: 519 LTLGFVMVTSFWLFFPQFLRFKADVRMFEEYAALGALVKNVTGDMITPL 565

[Close]

BLAST of Csor.00g187110 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 268.9 bits (686), Expect = 6.0e-72
Identity = 148/344 (43.02%), Postives = 203/344 (59.01%), Query Frame = 0


Query: 7   AFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRGTLGFFI 66
           +FI+   L+  SLC  F I   +P G  + +   P F +F  +P    ++HL G   FFI
Sbjct: 3   SFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFI 62

Query: 67  GWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDPLL--PL 126
            WL +FKLLLFA G+GPL       SL  FLAV+CLPI I     P      +     PL
Sbjct: 63  AWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGPL 122

Query: 127 SFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGVELLP 186
            +  K  F++L   A  +  +    VVL  + + IY  LEI++    A   A+  +EL P
Sbjct: 123 IYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEP 182

Query: 187 FFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMATFAVS 246
            F++PYL+ SLQDFWGRRWNL+ + ILRP V++P  +L  +V+G   + I+A   TF VS
Sbjct: 183 QFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLF-SVLGPNYSQILAAFGTFVVS 242

Query: 247 GVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVLTIGFV 306
           G+MHELI+FYMGRL P W++ WFFLI+G C   E+A+K  + GR+R P+ +S VLT+ FV
Sbjct: 243 GIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLTFV 302

Query: 307 MATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIGPLF 349
           M T+ WLF P+  R     + L+EYAA+GAF   V  +L   LF
Sbjct: 303 MVTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEVRRKLTAYLF 345

[Close]

BLAST of Csor.00g187110 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 232.3 bits (591), Expect = 6.2e-61
Identity = 139/338 (41.12%), Postives = 194/338 (57.40%), Query Frame = 0


Query: 1   MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 60
           M  E+ + I+       S+  C+     I +G  + L VLP   LFL +PL   ++H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP 120
           +  FF+ WL +FKL+LF+F +GPL      S+L RF+   C PI + ++  P N+     
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPL--FPVPSNLSRFVCFTCFPIKLQQNPKPQNQ----- 120

Query: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGV 180
           +    FA KL F  +      +K    P V+L+ + L IYL  EIL+     L    L  
Sbjct: 121 MPKWGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWC 180

Query: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMAT 240
           +L P F+EPYLS SLQDFWGRRWNL+   ILRPAV+ P R++AG  +    A  + V A+
Sbjct: 181 DLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFAS 240

Query: 241 FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVK-SAVGGRFRPPRVVSGVL 300
           F VSGV+HELI+FY  R +PT EV+ FF++HGVC AAE A K + +  R++  ++VS +L
Sbjct: 241 FLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMVSRLL 300

Query: 301 TIGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVK 338
           T+GFV+ T  WLFFP L R     R+ +E      FVK
Sbjct: 301 TVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVK 331

[Close]

BLAST of Csor.00g187110 vs. TAIR 10
Match: AT5G55360.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 219.5 bits (558), Expect = 4.2e-57
Identity = 138/339 (40.71%), Postives = 190/339 (56.05%), Query Frame = 0


Query: 1   MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 60
           M  E+  FI+       S+  C+ +   I  G  + L VLP   LFL  PL F  +H  G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP 120
            + FF+ WL +FKL+LF+F +GPL  +    +L RF+ + C PI   ++ +  N   P  
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPL--SPLPRTLSRFICITCFPIKPQQNPNIQNYKIPIW 120

Query: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGV 180
           L    FA K+   ++      +K    P ++L+F  L I+L LEI+     AL    LG 
Sbjct: 121 L----FAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALVFITLGC 180

Query: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMAT 240
           +L P  +EPYL+ SLQDFWGRRWNL+   ILRPAV+ P R++A   V    A  + V A 
Sbjct: 181 DLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMFLGVFAA 240

Query: 241 FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGR--FRPPRVVSGV 300
           F VSG +HE+++FY+ R  PT EV+WFFL+HGVC  AE+AVK +   R  +R    VS +
Sbjct: 241 FLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVSPTVSRL 300

Query: 301 LTIGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVK 338
           LT+GFV+ TS W FFP L+R     R+  E      FVK
Sbjct: 301 LTVGFVVVTSGWFFFP-LIRSGIIERLASEALMCIDFVK 332

[Close]

BLAST of Csor.00g187110 vs. TAIR 10
Match: AT5G55370.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 211.1 bits (536), Expect = 1.5e-54
Identity = 133/336 (39.58%), Postives = 190/336 (56.55%), Query Frame = 0


Query: 1   MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 60
           M  E+   I+       S+  C+ I + I  G L+ L +LP F +FL +PL F ++H   
Sbjct: 1   MEEELRNLIKVWISALISISYCYYISSKISKGVLRLLSLLPIFIIFLLLPLFFSSVHFCV 60

Query: 61  TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP 120
             GFF  WL +FKL LFAF + PL  +   S+L RF   AC PI I ++ S  NR    P
Sbjct: 61  ISGFFFTWLANFKLFLFAFDQEPL--SPLPSNLTRFFCFACFPIKINKNPS-SNRIHNKP 120

Query: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNV----VLIFFCLLIYLLLEILIGGATALAEA 180
           +     A KL  LI +F    ++N +   +     L  F + +YL  E+++    AL   
Sbjct: 121 MSKWVLAFKL--LIFSFLLHVYRNNYDSGLSRFAFLALFTIHVYLEAELILVFVGALMSM 180

Query: 181 VLGVELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIA 240
           +LG E+ P F++PYL+ SLQ+FW RRWNL+   +LRPAV  P ++    ++G   A    
Sbjct: 181 LLGCEMEPVFNDPYLATSLQEFWSRRWNLMVPAVLRPAVHIPVQRFCAPLLGLHRAFYAG 240

Query: 241 VMATFAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFR----PP 300
           ++ATF VSG+MHELIYFY+ R +PTWEV+ FFL+HGV    E+A+K     R R    P 
Sbjct: 241 MLATFIVSGLMHELIYFYVIRKSPTWEVTCFFLLHGVVTCLEIAMK-----RMRWLPTPR 300

Query: 301 RVVSGVLTIGFVMATSFWLFFPQLLRFKADVRMLEE 329
           R VSG+    F++ T+ WLF+PQ+LR     R++ E
Sbjct: 301 RAVSGLAITVFLLVTAGWLFYPQMLRNDVHKRVISE 326

[Close]

BLAST of Csor.00g187110 vs. TAIR 10
Match: AT5G55380.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 206.5 bits (524), Expect = 3.6e-53
Identity = 128/305 (41.97%), Postives = 173/305 (56.72%), Query Frame = 0


Query: 18  SLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRGTLGFFIGWLGSFKLLLF 77
           SL  C+ I + +  G L+ L +LP   LFL +PL    +H       F+ WL +FKLLLF
Sbjct: 18  SLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCAISVLFLSWLANFKLLLF 77

Query: 78  AFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSP---PNRSDPDPLLPLSFAAKLGFLI 137
           AF +GPL        L RF+  ACLPI I +  SP   PN   P P+     A K+  L 
Sbjct: 78  AFDEGPL--FPLPPKLSRFICFACLPIKIRQDPSPNAIPN-LHPKPMPKWVLAVKILVLG 137

Query: 138 LTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGVELLPFFDEPYLSDS 197
           +      +++     VVL  +CL IYL +E+++    A+   +LG  + P F+EPYL+ S
Sbjct: 138 VLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVVSTLLGCNIEPVFNEPYLATS 197

Query: 198 LQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMATFAVSGVMHELIYFY 257
           LQDFW RRWNL+ S +LR  V  P ++    ++    A    VMA+F VSG+MHEL+YFY
Sbjct: 198 LQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFAGVMASFFVSGLMHELLYFY 257

Query: 258 MGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPP-RVVSGVLTIGFVMATSFWLFF 317
           M R  PTWEV+ FF++HG   A E+AVK       RPP R VSG++ + FV  T  WLF 
Sbjct: 258 MIRKPPTWEVTCFFVLHGAATATEIAVKRTQW--LRPPHRAVSGLVVLTFVSVTGVWLFL 317

Query: 318 PQLLR 319
            Q+LR
Sbjct: 318 AQVLR 317

The following BLAST results are available for this feature:

BLAST of Csor.00g187110 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SV07	8.4e-71	43.02	Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE...	[more]
Q9FJ75	8.7e-60	41.12	Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX...	[more]
Q9XGY6	4.8e-58	38.22	Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 ...	[more]
Q9FJ74	5.9e-56	40.71	Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX...	[more]
Q9FJ73	2.1e-53	39.58	Probable long-chain-alcohol O-fatty-acyltransferase 2 OS=Arabidopsis thaliana OX...	[more]

BLAST of Csor.00g187110 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022934181.1	3.61e-249	100.00	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata] >KAG6581558.1 Acy...	[more]
KAG7018062.1	3.56e-246	100.00	Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. arg...	[more]
XP_022982637.1	1.02e-239	96.61	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima]	[more]
XP_023528035.1	2.39e-238	96.05	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_038905685.1	2.79e-191	76.84	acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida]	[more]

BLAST of Csor.00g187110 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F144	1.75e-249	100.00	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1IX47	4.92e-240	96.61	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1GBB6	2.96e-172	72.11	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1KCL2	6.92e-171	72.11	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1DI64	1.02e-170	72.21	acyl-CoA--sterol O-acyltransferase 1-like OS=Momordica charantia OX=3673 GN=LOC1...	[more]

BLAST of Csor.00g187110 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51970.1	6.0e-72	43.02	acyl-CoA sterol acyl transferase 1	[more]
AT5G55350.1	6.2e-61	41.12	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55360.1	4.2e-57	40.71	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55370.1	1.5e-54	39.58	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55380.1	3.6e-53	41.97	MBOAT (membrane bound O-acyl transferase) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032805	Wax synthase domain	PFAM	PF13813	MBOAT_2	coord: 184..269 e-value: 4.7E-16 score: 58.8
IPR017088	Wax synthase, Magnoliopsida	PIRSF	PIRSF037006	Wax_synthase	coord: 1..352 e-value: 2.4E-111 score: 370.2
IPR044851	Wax synthase	PANTHER	PTHR31595	LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED	coord: 3..341
None	No IPR available	PANTHER	PTHR31595:SF46	ACYL-COA--STEROL O-ACYLTRANSFERASE 1	coord: 3..341

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g187110.m01	Csor.00g187110.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008374	O-acyltransferase activity