Csor.00g186560 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g186560
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	4-coumarate:CoA ligase
Location	Csor_Chr14: 6791816 .. 6794142 (-)
RNA-Seq Expression	Csor.00g186560
Synteny	Csor.00g186560

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGTTGAAGCTCTTCAGAATGACTTCATTTTCCGCTCCAAACTGCCCGATATTTACATTCCTAACCACCTTCCTCTGCACTCCTATTGCCTACACGAAAACCTCGCCAAAATCGGCCACCGGACTTGTCTAATCAACTCCGTCACCGGCGAATCCTTTACCTACCACGACGTCGACCTCGCCGCACGCAAGGTCGCCTCGGGATTGAACAAACTCGGCATTGCTCAAGGCGATGTGATTATGCTCCTCCTCCAGAATTCGCCGGAATTCGTCTTCGCTTTCCTTGGTGCGTCGTATCGCGGCGCGATTATGACGGCGGCGAATCCTTTCTTCACGGCGGCGGAGATCGCAAAGCAAGCAAAAGGATCGAATGCGAAATTAATCGTGACGCAATCGTCGTATTATGAAAAAGTGAAGGAAATAACAGAGGAATTACCTGATGTTAAAATCATGACCGTCGATTCTCCGCCGGATGGTTGTTTATCGTTCGCCGATCTAATTCAGGCCGACGAACGAGAGATGCCGACTGTTGAAATCAATCCGGACGATGTGGTTGCGCTGCCGTATTCCTCTGGAACCACCGGATTGCCGAAAGGAGTGATGCTTACACACAGAGGATTAGTGACGAGTGTGGCTCAGCAAGTGGACGGCGAAAATCCGAATCTGTACTATCGAAATGAGGACGTGATTCTGTGCGTTTTGCCGCTCTTCCATATCTATTCGCTTAATTCCGTCCTCCTCTGCGGATTACGGGCGGGGACCACCATTTTGATTATGCCTAAATTCGAGATCGGTTCGCTTTTGCAATTGGTGCAGAAATTCAAAGTGTCGATTGCGCCGATTGTGCCGCCGATTGTTTTGGCCATCGCGAAGTCGCCGGACCTCGACAAGTACGATCTTTCGTCTATCAGAGTTATCAAATGCGGCGGCGCTCCGCTTGGGAAGGAGCTTGAAGATACCGTGAGAGCCAAGTTTCCCAAGGCTGTGCTCGGCCAGGTAATGCAAATTCATTTAAAAAAGAATTAATAAATTAAAAATCATTTTAAACTTTAAATTATATTAAAATTTAGATATTAAATTTGTAATAATTATATTGAATTGTGGAAATTTGTGTTTATTATGTGTAATTATGATTATTTTTTTATTGGTATTTAATAAAAAAAATATCTATTTTTGGAAGGATAATTAAAAGACTTAAATGGAGACGACACCCCTAAGTCAAAGCAGAAATCCACGTGTAATTTTTTAATTACAATAAAATTAATTCATTTTCATTTTTTTTAACGTTATATAATTTTTAAAAAATTTAAATATATTTTAGGGACCGTTAAATATAAATTCAAGGTTGAATAATTAAAAATAATTTAAATTCAAAACTAAAGCTAACTAAATTTATAATTTAATTTTTGGATCAGATGTAATCCTAGCCGTTGGATCATCAAGATTGATCAGAGAAATTCTGTTGATTGCAGGGATACGGAATGACGGAGGCCGGCCCAGTGCTAACGATGGGTTTAGCATTTGCAAAGGAGCCATTTCCGATGAAACCCGGCGCCTGCGGCACCGTCGTGAGAAACGCCGAGATGAAGATCGTCGACACCGAAAACGGCGGTTCGTTGCCTCGAAACACGCCGGGAGAGATCTGCATCAGAGGCGATCAAATCATGAAAGGGTATCTGAACAATCCGGAGGCGACAGCCGCCACAATCGACAAGGATGGGTGGTTGCACACCGGCGACATCGGATTCATCGACGACGACGACGAGCTGTTCATCGTCGATCGATTGAAGGAACTTATAAAGTATAAGGGATTTCAAGTGGCTCCGGCGGAAGTGGAAGCTCTGTTGCTCACTCATCCGGCAATCTCTGATGCCGCCGTCGTTCCGTAAGAGAATTGATTGTGATTTGATTTCCGAATTGGGTTTGTGGAAATCGCGGATAATTATGGGTTAATTGAATTGCAGAATGAAAGATGAAGAAGCCGGAGAGGTTCCCGTTGCGTTCGTGGTGAGATTGAAGAACTCTGAAGTAACTGAGGATGAAATTAAACAATTCATATCAAAGCAGGTACACTTTTTTCAAATTCATAGGAATCAACCGATCACGATATGATCCTCCTCCATAATGATATGATATTGTCCACTATGAGCTGTGAATTCGGGATGAAATCATTGACTCTGTTCCGTTATTGTTTGCAGGTGGTATTCTACAAGAGAATCAAGAGGGCGTTTATGATAGACGCAATTCCGAAATCGCCGTCGGGAAAGATCTTGAGGAAGGAGTTGAGGGCAAAATTGGCAACTGGTTTTCCAAATTCTAATTAA

mRNA sequence

ATGGCTGTTGAAGCTCTTCAGAATGACTTCATTTTCCGCTCCAAACTGCCCGATATTTACATTCCTAACCACCTTCCTCTGCACTCCTATTGCCTACACGAAAACCTCGCCAAAATCGGCCACCGGACTTGTCTAATCAACTCCGTCACCGGCGAATCCTTTACCTACCACGACGTCGACCTCGCCGCACGCAAGGTCGCCTCGGGATTGAACAAACTCGGCATTGCTCAAGGCGATGTGATTATGCTCCTCCTCCAGAATTCGCCGGAATTCGTCTTCGCTTTCCTTGGTGCGTCGTATCGCGGCGCGATTATGACGGCGGCGAATCCTTTCTTCACGGCGGCGGAGATCGCAAAGCAAGCAAAAGGATCGAATGCGAAATTAATCGTGACGCAATCGTCGTATTATGAAAAAGTGAAGGAAATAACAGAGGAATTACCTGATGTTAAAATCATGACCGTCGATTCTCCGCCGGATGGTTGTTTATCGTTCGCCGATCTAATTCAGGCCGACGAACGAGAGATGCCGACTGTTGAAATCAATCCGGACGATGTGGTTGCGCTGCCGTATTCCTCTGGAACCACCGGATTGCCGAAAGGAGTGATGCTTACACACAGAGGATTAGTGACGAGTGTGGCTCAGCAAGTGGACGGCGAAAATCCGAATCTGTACTATCGAAATGAGGACGTGATTCTGTGCGTTTTGCCGCTCTTCCATATCTATTCGCTTAATTCCGTCCTCCTCTGCGGATTACGGGCGGGGACCACCATTTTGATTATGCCTAAATTCGAGATCGGTTCGCTTTTGCAATTGGTGCAGAAATTCAAAGTGTCGATTGCGCCGATTGTGCCGCCGATTGTTTTGGCCATCGCGAAGTCGCCGGACCTCGACAAGTACGATCTTTCGTCTATCAGAGTTATCAAATGCGGCGGCGCTCCGCTTGGGAAGGAGCTTGAAGATACCGTGAGAGCCAAGTTTCCCAAGGCTGTGCTCGGCCAGGGATACGGAATGACGGAGGCCGGCCCAGTGCTAACGATGGGTTTAGCATTTGCAAAGGAGCCATTTCCGATGAAACCCGGCGCCTGCGGCACCGTCGTGAGAAACGCCGAGATGAAGATCGTCGACACCGAAAACGGCGGTTCGTTGCCTCGAAACACGCCGGGAGAGATCTGCATCAGAGGCGATCAAATCATGAAAGGGTATCTGAACAATCCGGAGGCGACAGCCGCCACAATCGACAAGGATGGGTGGTTGCACACCGGCGACATCGGATTCATCGACGACGACGACGAGCTGTTCATCGTCGATCGATTGAAGGAACTTATAAAGTATAAGGGATTTCAAGTGGCTCCGGCGGAAGTGGAAGCTCTGTTGCTCACTCATCCGGCAATCTCTGATGCCGCCGTCGTTCCAATGAAAGATGAAGAAGCCGGAGAGGTTCCCGTTGCGTTCGTGGTGAGATTGAAGAACTCTGAAGTAACTGAGGATGAAATTAAACAATTCATATCAAAGCAGGTGGTATTCTACAAGAGAATCAAGAGGGCGTTTATGATAGACGCAATTCCGAAATCGCCGTCGGGAAAGATCTTGAGGAAGGAGTTGAGGGCAAAATTGGCAACTGGTTTTCCAAATTCTAATTAA

Coding sequence (CDS)

ATGGCTGTTGAAGCTCTTCAGAATGACTTCATTTTCCGCTCCAAACTGCCCGATATTTACATTCCTAACCACCTTCCTCTGCACTCCTATTGCCTACACGAAAACCTCGCCAAAATCGGCCACCGGACTTGTCTAATCAACTCCGTCACCGGCGAATCCTTTACCTACCACGACGTCGACCTCGCCGCACGCAAGGTCGCCTCGGGATTGAACAAACTCGGCATTGCTCAAGGCGATGTGATTATGCTCCTCCTCCAGAATTCGCCGGAATTCGTCTTCGCTTTCCTTGGTGCGTCGTATCGCGGCGCGATTATGACGGCGGCGAATCCTTTCTTCACGGCGGCGGAGATCGCAAAGCAAGCAAAAGGATCGAATGCGAAATTAATCGTGACGCAATCGTCGTATTATGAAAAAGTGAAGGAAATAACAGAGGAATTACCTGATGTTAAAATCATGACCGTCGATTCTCCGCCGGATGGTTGTTTATCGTTCGCCGATCTAATTCAGGCCGACGAACGAGAGATGCCGACTGTTGAAATCAATCCGGACGATGTGGTTGCGCTGCCGTATTCCTCTGGAACCACCGGATTGCCGAAAGGAGTGATGCTTACACACAGAGGATTAGTGACGAGTGTGGCTCAGCAAGTGGACGGCGAAAATCCGAATCTGTACTATCGAAATGAGGACGTGATTCTGTGCGTTTTGCCGCTCTTCCATATCTATTCGCTTAATTCCGTCCTCCTCTGCGGATTACGGGCGGGGACCACCATTTTGATTATGCCTAAATTCGAGATCGGTTCGCTTTTGCAATTGGTGCAGAAATTCAAAGTGTCGATTGCGCCGATTGTGCCGCCGATTGTTTTGGCCATCGCGAAGTCGCCGGACCTCGACAAGTACGATCTTTCGTCTATCAGAGTTATCAAATGCGGCGGCGCTCCGCTTGGGAAGGAGCTTGAAGATACCGTGAGAGCCAAGTTTCCCAAGGCTGTGCTCGGCCAGGGATACGGAATGACGGAGGCCGGCCCAGTGCTAACGATGGGTTTAGCATTTGCAAAGGAGCCATTTCCGATGAAACCCGGCGCCTGCGGCACCGTCGTGAGAAACGCCGAGATGAAGATCGTCGACACCGAAAACGGCGGTTCGTTGCCTCGAAACACGCCGGGAGAGATCTGCATCAGAGGCGATCAAATCATGAAAGGGTATCTGAACAATCCGGAGGCGACAGCCGCCACAATCGACAAGGATGGGTGGTTGCACACCGGCGACATCGGATTCATCGACGACGACGACGAGCTGTTCATCGTCGATCGATTGAAGGAACTTATAAAGTATAAGGGATTTCAAGTGGCTCCGGCGGAAGTGGAAGCTCTGTTGCTCACTCATCCGGCAATCTCTGATGCCGCCGTCGTTCCAATGAAAGATGAAGAAGCCGGAGAGGTTCCCGTTGCGTTCGTGGTGAGATTGAAGAACTCTGAAGTAACTGAGGATGAAATTAAACAATTCATATCAAAGCAGGTGGTATTCTACAAGAGAATCAAGAGGGCGTTTATGATAGACGCAATTCCGAAATCGCCGTCGGGAAAGATCTTGAGGAAGGAGTTGAGGGCAAAATTGGCAACTGGTTTTCCAAATTCTAATTAA

Protein sequence

MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVDLAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEINPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHIYSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYDLSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEVPVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLATGFPNSN

Homology

[Close]

BLAST of Csor.00g186560 vs. ExPASy Swiss-Prot
Match: O24146 (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 808.1 bits (2086), Expect = 5.9e-233
Identity = 407/544 (74.82%), Postives = 457/544 (84.01%), Query Frame = 0


Query: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60
           M  +  Q D IFRSKLPDIYIPNHLPLHSYC  EN+++   R CLIN    + +TY DV+
Sbjct: 1   MEKDTKQVDIIFRSKLPDIYIPNHLPLHSYCF-ENISEFSSRPCLINGANKQIYTYADVE 60

Query: 61  LAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           L +RKVA+GL+K GI   D IM+LL NSPEFVFAF+GASY GAI T ANP FT AE+ KQ
Sbjct: 61  LNSRKVAAGLHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 120

Query: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180
           AK S+AK+IVTQ+ +  KVK+   E  DVKI+ +DS P+GCL F+ L QA+E ++P VEI
Sbjct: 121 AKASSAKIIVTQACHVNKVKDYAFE-NDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEI 180

Query: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240
            PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDGENPNLY  +EDV+LCVLPLFHI
Sbjct: 181 QPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLR G  ILIM KF+I S L+L+Q++KV+I P VPPIVLAIAKSP +D YD
Sbjct: 241 YSLNSVLLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSS+R +  G APLGKELEDTVRAKFP A LGQGYGMTEAGPVL M LAFAKEPF +K G
Sbjct: 301 LSSVRTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSG 360

Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVD + G SLPRN  GEICIRGDQIMKGYLN+PEATA TIDK+GWL+T
Sbjct: 361 ACGTVVRNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480
           GDIG+IDDDDELFIVDRLKELIKYKGFQVAPAE+EALLL HP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540
           PVAFVVR   S +TEDE+K FISKQV+FYKRIKR F +DAIPKSPSGKILRK+LRAKLA 
Sbjct: 481 PVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAA 540

Query: 541 GFPN 545
           G PN
Sbjct: 541 GLPN 542

[Close]

BLAST of Csor.00g186560 vs. ExPASy Swiss-Prot
Match: P14912 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 805.1 bits (2078), Expect = 5.0e-232
Identity = 400/537 (74.49%), Postives = 456/537 (84.92%), Query Frame = 0


Query: 5   ALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVDLAAR 64
           A + D IFRSKLPDIYIP HLPLH+YC  EN++K+G ++CLIN  TGE+FTY  V+L +R
Sbjct: 6   APKEDLIFRSKLPDIYIPKHLPLHTYCF-ENISKVGDKSCLINGATGETFTYSQVELLSR 65

Query: 65  KVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGS 124
           KVASGLNKLGI QGD IMLLL NSPE+ FAFLGASYRGAI T ANPFFT+AE+ KQ K S
Sbjct: 66  KVASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKAS 125

Query: 125 NAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEINPDD 184
            AKLI+TQ+ Y +KVK+   E  +++I+ +D  P  CL F+ L++ADE EMP V IN DD
Sbjct: 126 QAKLIITQACYVDKVKDYAAE-KNIQIICIDDAPQDCLHFSKLMEADESEMPEVVINSDD 185

Query: 185 VVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHIYSLN 244
           VVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDG+NPNLY  +EDV++C+LPLFHIYSLN
Sbjct: 186 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLN 245

Query: 245 SVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYDLSSI 304
           +VL CGLRAG TILIM KF+I   L+L+QK+KV+I P VPPIVLAIAKSP +DKYDLSS+
Sbjct: 246 AVLCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSV 305

Query: 305 RVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGT 364
           R +  G APLGKELED VRAKFP A LGQGYGMTEAGPVL M LAFAKEP+ +K GACGT
Sbjct: 306 RTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGT 365

Query: 365 VVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIG 424
           VVRNAEMKIVD E   SLPRN  GEICIRGDQIMKGYLN+PE+T  TID++GWLHTGDIG
Sbjct: 366 VVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIG 425

Query: 425 FIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEVPVAF 484
           FIDDDDELFIVDRLKE+IKYKGFQVAPAE+EALLLTHP ISDAAVVPM DE+AGEVPVAF
Sbjct: 426 FIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAF 485

Query: 485 VVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLATG 542
           VVR      TE+EIKQF+SKQVVFYKRI R F +DAIPKSPSGKILRK+LRA++A+G
Sbjct: 486 VVRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRARIASG 540

[Close]

BLAST of Csor.00g186560 vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 8.6e-232
Identity = 397/536 (74.07%), Postives = 449/536 (83.77%), Query Frame = 0


Query: 9   DFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVDLAARKVAS 68
           D IFRSKLPDIYIP HLPLHSYC  EN+++   R CLIN    + +TY +V+L  RKVA 
Sbjct: 14  DLIFRSKLPDIYIPKHLPLHSYCF-ENISEFSSRPCLINGANDQIYTYAEVELTCRKVAV 73

Query: 69  GLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSNAKL 128
           GLNKLGI Q D IM+LL NSPEFVFAF+GASY GAI T ANP FT AE+ KQAK S+AK+
Sbjct: 74  GLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKASSAKI 133

Query: 129 IVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEINPDDVVAL 188
           I+TQS +  KVK+   E  DVK++ +DS P+GCL F++L Q+DE E+P V+I PDDVVAL
Sbjct: 134 IITQSCFVGKVKDYASE-NDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQPDDVVAL 193

Query: 189 PYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHIYSLNSVLL 248
           PYSSGTTGLPKGVMLTH+GLVTSVAQQVDGEN NLY  +EDV++CVLPLFHIYSLNS+LL
Sbjct: 194 PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLNSILL 253

Query: 249 CGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYDLSSIRVIK 308
           CGLR G  ILIM KF+I   L+L+QK+KVSI P VPPIVLAIAKSP +D YDLSS+R + 
Sbjct: 254 CGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSVRTVM 313

Query: 309 CGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRN 368
            G APLGKELED VR KFP A LGQGYGMTEAGPVL M LAFAKEPF +K GACGTVVRN
Sbjct: 314 SGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRN 373

Query: 369 AEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDD 428
           AEMKIVD + G SLPRN PGEICIRGDQIMKGYLN+PEAT  TIDK+GWLHTGDIGFID+
Sbjct: 374 AEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIGFIDE 433

Query: 429 DDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEVPVAFVVRL 488
           DDELFIVDRLKELIKYKGFQVAPAE+EALLL HP ISDAAVVPMKDE+AGEVPVAFVVR 
Sbjct: 434 DDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRS 493

Query: 489 KNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLATGFPN 545
             S +TEDE+K FISKQV+FYKR+KR F ++ +PKSPSGKILRK+LRA+LA G PN
Sbjct: 494 NGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAGVPN 547

[Close]

BLAST of Csor.00g186560 vs. ExPASy Swiss-Prot
Match: P14913 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1)

HSP 1 Score: 803.1 bits (2073), Expect = 1.9e-231
Identity = 400/537 (74.49%), Postives = 456/537 (84.92%), Query Frame = 0


Query: 5   ALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVDLAAR 64
           A + D IFRSKLPDIYIP HLPLH+YC  EN++K+G ++CLIN  TGE+FTY  V+L +R
Sbjct: 6   APKEDLIFRSKLPDIYIPKHLPLHTYCF-ENISKVGDKSCLINGATGETFTYSQVELLSR 65

Query: 65  KVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGS 124
           KVASGLNKLGI QGD IMLLL NSPE+ FAFLGASYRGAI T ANPFFT+AE+ KQ K S
Sbjct: 66  KVASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKAS 125

Query: 125 NAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEINPDD 184
            AKLI+TQ+ Y +KVK+   E  +++I+ +D  P  CL F+ L++ADE EMP V I+ DD
Sbjct: 126 LAKLIITQACYVDKVKDYAAE-KNIQIICIDDAPQDCLHFSKLMEADESEMPEVVIDSDD 185

Query: 185 VVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHIYSLN 244
           VVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDG+NPNLY  +EDV++C+LPLFHIYSLN
Sbjct: 186 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLN 245

Query: 245 SVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYDLSSI 304
           +VL CGLRAG TILIM KF+I   L+L+QK+KV+I P VPPIVLAIAKSP +DKYDLSS+
Sbjct: 246 AVLCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSV 305

Query: 305 RVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGT 364
           R +  G APLGKELED VRAKFP A LGQGYGMTEAGPVL M LAFAKEP+ +K GACGT
Sbjct: 306 RTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGT 365

Query: 365 VVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIG 424
           VVRNAEMKIVD E   SLPRN  GEICIRGDQIMKGYLN+PE+T  TID++GWLHTGDIG
Sbjct: 366 VVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIG 425

Query: 425 FIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEVPVAF 484
           FIDDDDELFIVDRLKE+IKYKGFQVAPAE+EALLLTHP ISDAAVVPM DE+AGEVPVAF
Sbjct: 426 FIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAF 485

Query: 485 VVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLATG 542
           VVR      TE+EIKQF+SKQVVFYKRI R F +DAIPKSPSGKILRK+LRAK+A+G
Sbjct: 486 VVRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRAKIASG 540

[Close]

BLAST of Csor.00g186560 vs. ExPASy Swiss-Prot
Match: M4ISH0 (4-coumarate--CoA ligase CCL1 OS=Humulus lupulus OX=3486 GN=CCL1 PE=1 SV=1)

HSP 1 Score: 798.1 bits (2060), Expect = 6.1e-230
Identity = 397/536 (74.07%), Postives = 454/536 (84.70%), Query Frame = 0


Query: 7   QNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVDLAARKV 66
           Q +FIFRSKLPDIYIPNHLPLHSYC  EN+++   R CLIN  TGE  TY DVDL +RKV
Sbjct: 10  QEEFIFRSKLPDIYIPNHLPLHSYCF-ENISQFKDRPCLINGATGEIITYADVDLTSRKV 69

Query: 67  ASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSNA 126
           A+GL+KLGI QGDVIMLLLQNSPEFV+AFL ASY GAI+T ANPF+T AE+AKQA  S  
Sbjct: 70  AAGLDKLGIKQGDVIMLLLQNSPEFVYAFLAASYIGAIITTANPFYTPAEVAKQAAASKT 129

Query: 127 KLIVTQSSYYEKVKEITEELPDVKIMTVDSPP--DGCLSFADLIQADEREMPTVEINPDD 186
           KL++T + Y +KVKE T     VK+M VD+PP    CL F++L QADE E+P V+I+PDD
Sbjct: 130 KLVITLAGYIDKVKEFTGGESGVKVMCVDAPPPESECLHFSELTQADETEIPAVKIHPDD 189

Query: 187 VVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHIYSLN 246
           VVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDG+NPNLY+   DVILCVLPLFHIYSLN
Sbjct: 190 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFHQNDVILCVLPLFHIYSLN 249

Query: 247 SVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYDLSSI 306
           S+LLCGLR G  ILIM KFEI  LL+L++KFKV+IAP VPPIVL++AK PDL +YDLSSI
Sbjct: 250 SILLCGLRVGAAILIMQKFEISKLLELIEKFKVTIAPFVPPIVLSVAKCPDLHRYDLSSI 309

Query: 307 RVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGT 366
           R +  GGAP+GKELED V+ K P A LGQGYGMTEAGPVL+M LAFAKEPFP+K GACGT
Sbjct: 310 RTVMSGGAPMGKELEDAVKEKLPHAKLGQGYGMTEAGPVLSMCLAFAKEPFPIKSGACGT 369

Query: 367 VVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIG 426
           VVRNAEMKIVD + G SLPRN  GEICIRG QIMKGY+N+ EAT  TID+ GWLHTGDIG
Sbjct: 370 VVRNAEMKIVDPDTGASLPRNQSGEICIRGKQIMKGYINDAEATKGTIDEGGWLHTGDIG 429

Query: 427 FIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEVPVAF 486
           FID+DDELFIVDRLKELIKYKGFQVAPAE+E++L++HP I+DAAVVPMKDE AGEVPVAF
Sbjct: 430 FIDNDDELFIVDRLKELIKYKGFQVAPAELESMLISHPNITDAAVVPMKDEAAGEVPVAF 489

Query: 487 VVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 541
           VVR   S++TE++IKQ+ISKQVVFYKRI +AF I+ IPK+PSGKILRK LRAKL T
Sbjct: 490 VVRSNGSKITEEDIKQYISKQVVFYKRINKAFFIEEIPKNPSGKILRKILRAKLVT 544

[Close]

BLAST of Csor.00g186560 vs. NCBI nr
Match: KAG6581611.1 (4-coumarate--CoA ligase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1076 bits (2782), Expect = 0.0
Identity = 546/546 (100.00%), Postives = 546/546 (100.00%), Query Frame = 0


Query: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60
           MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 61  LAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           LAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180
           AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240
           NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480
           GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540
           PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540

Query: 541 GFPNSN 546
           GFPNSN
Sbjct: 541 GFPNSN 546

[Close]

BLAST of Csor.00g186560 vs. NCBI nr
Match: XP_022935389.1 (4-coumarate--CoA ligase 1-like [Cucurbita moschata])

HSP 1 Score: 1073 bits (2775), Expect = 0.0
Identity = 544/546 (99.63%), Postives = 545/546 (99.82%), Query Frame = 0


Query: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60
           MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 61  LAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           LAARKVASGLNKLGIAQGDVIML+LQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLILQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180
           AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240
           NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480
           GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540
           PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLA 
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAA 540

Query: 541 GFPNSN 546
           GFPNSN
Sbjct: 541 GFPNSN 546

[Close]

BLAST of Csor.00g186560 vs. NCBI nr
Match: XP_023526871.1 (4-coumarate--CoA ligase 1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1070 bits (2768), Expect = 0.0
Identity = 543/546 (99.45%), Postives = 544/546 (99.63%), Query Frame = 0


Query: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60
           MAVEALQNDFIFRSKLP IYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD
Sbjct: 1   MAVEALQNDFIFRSKLPHIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 61  LAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           LAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180
           AKGSNAKLIVTQSSYYEKVKEITEE+PDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEEVPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240
           NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRVIKCGGAPLGKELEDTVR KFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRTKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480
           GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540
           PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540

Query: 541 GFPNSN 546
           GFPNSN
Sbjct: 541 GFPNSN 546

[Close]

BLAST of Csor.00g186560 vs. NCBI nr
Match: XP_022983944.1 (4-coumarate--CoA ligase 1-like [Cucurbita maxima])

HSP 1 Score: 1061 bits (2745), Expect = 0.0
Identity = 539/546 (98.72%), Postives = 543/546 (99.45%), Query Frame = 0


Query: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60
           MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLIN+VT ESFTYHDVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINAVTAESFTYHDVD 60

Query: 61  LAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           LAARKVASGLNKLGIAQGDVI+LLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVILLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180
           AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240
           NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRVIKCGGAPLGKELED+VRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDSVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTENG SLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGCSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480
           GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAE+EALLLTHPAISDAAVVPMKDEEAGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540
           PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540

Query: 541 GFPNSN 546
           GFP SN
Sbjct: 541 GFPISN 546

[Close]

BLAST of Csor.00g186560 vs. NCBI nr
Match: XP_023526872.1 (4-coumarate--CoA ligase 1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 991 bits (2563), Expect = 0.0
Identity = 502/505 (99.41%), Postives = 503/505 (99.60%), Query Frame = 0


Query: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60
           MAVEALQNDFIFRSKLP IYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD
Sbjct: 1   MAVEALQNDFIFRSKLPHIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 61  LAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           LAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180
           AKGSNAKLIVTQSSYYEKVKEITEE+PDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEEVPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240
           NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRVIKCGGAPLGKELEDTVR KFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRTKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480
           GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVRLKNSEVTEDEIKQFISKQ 505
           PVAFVVRLKNSEVTEDEIKQFISKQ
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQ 505

[Close]

BLAST of Csor.00g186560 vs. ExPASy TrEMBL
Match: A0A6J1F599 (4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111442288 PE=4 SV=1)

HSP 1 Score: 1073 bits (2775), Expect = 0.0
Identity = 544/546 (99.63%), Postives = 545/546 (99.82%), Query Frame = 0


Query: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60
           MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 61  LAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           LAARKVASGLNKLGIAQGDVIML+LQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLILQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180
           AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240
           NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480
           GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540
           PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLA 
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAA 540

Query: 541 GFPNSN 546
           GFPNSN
Sbjct: 541 GFPNSN 546

[Close]

BLAST of Csor.00g186560 vs. ExPASy TrEMBL
Match: A0A6J1J912 (4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482411 PE=4 SV=1)

HSP 1 Score: 1061 bits (2745), Expect = 0.0
Identity = 539/546 (98.72%), Postives = 543/546 (99.45%), Query Frame = 0


Query: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60
           MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLIN+VT ESFTYHDVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINAVTAESFTYHDVD 60

Query: 61  LAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           LAARKVASGLNKLGIAQGDVI+LLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVILLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180
           AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240
           NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRVIKCGGAPLGKELED+VRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDSVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTENG SLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGCSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480
           GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAE+EALLLTHPAISDAAVVPMKDEEAGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540
           PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540

Query: 541 GFPNSN 546
           GFP SN
Sbjct: 541 GFPISN 546

[Close]

BLAST of Csor.00g186560 vs. ExPASy TrEMBL
Match: A0A5A7SWF8 (4-coumarate--CoA ligase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1199G00110 PE=4 SV=1)

HSP 1 Score: 974 bits (2519), Expect = 0.0
Identity = 490/546 (89.74%), Postives = 516/546 (94.51%), Query Frame = 0


Query: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60
           M +E ++ND IFRSKLPDIYIPNHLPLHSYCL EN AKIGHRTCLIN VTGESFT++DVD
Sbjct: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60

Query: 61  LAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           L ARKVASGLNKLGI + DVIMLLL NSPEFVFAFLGAS+ GAIMTAANPFFTAAEIAKQ
Sbjct: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180
           AKGS AKLI+TQSSYYEK+KEITEELP+VKIMTVDSP DGCL F DLI+ADERE+P VEI
Sbjct: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180

Query: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240
           +PDDVVALPYSSGTTGLPKGVMLTH+ LVTSVAQQVDGENPNLYY NEDVILCVLPLFHI
Sbjct: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAG+TILIMPKFEIG LLQLV+K+ V++APIVPPIVLAIAKSP+L+KYD
Sbjct: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRVIK GGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTE G SLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480
           GDIGFID+DDELFIVDRLKELIKYKGFQVAPAE+EALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540
           PVAFVV+LKNSE TEDEIKQFISKQVVFYK+I R F IDAIPKSPSGKILRKELRAKLA 
Sbjct: 481 PVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAA 540

Query: 541 GFPNSN 546
            FPNSN
Sbjct: 541 AFPNSN 546

[Close]

BLAST of Csor.00g186560 vs. ExPASy TrEMBL
Match: A0A6J1G9T7 (4-coumarate--CoA ligase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452267 PE=4 SV=1)

HSP 1 Score: 965 bits (2494), Expect = 0.0
Identity = 489/543 (90.06%), Postives = 513/543 (94.48%), Query Frame = 0


Query: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60
           MA E + +DFIFRSKLPDIYIP HLPLHSYCL E +A+IGHRTCLIN+VTGESFTY DVD
Sbjct: 1   MATETV-HDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHRTCLINAVTGESFTYSDVD 60

Query: 61  LAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           LAARK ASGL KLGIA+GDVIMLLL NSPEFVFAFLGASY GAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180
           AKGS AKLI+TQSSYYEKVKEITE+L D KIMTVDSP  GCLSFADLIQ  + ++P VEI
Sbjct: 121 AKGSKAKLIITQSSYYEKVKEITEQLSDAKIMTVDSPSLGCLSFADLIQGGDHKIPAVEI 180

Query: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240
           +PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDGENPNLYYRN+DVILCVLPLFHI
Sbjct: 181 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAG TILIMPKFEIGSLLQLV+K++VSIAPIVPPIVLAIAKSPDL+KYD
Sbjct: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIR+IK GGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAE+KIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATID +GWLHT
Sbjct: 361 ACGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDSEGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480
           GDIG IDDDDELFIVDRLKELIKYKGFQVAPAE+EALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540
           PVAFVV+LKNSEVTEDEIKQFISKQVVFYKRI R F IDAIPKSPSGKILRKELRAKLA 
Sbjct: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA 540

Query: 541 GFP 543
            FP
Sbjct: 541 DFP 542

[Close]

BLAST of Csor.00g186560 vs. ExPASy TrEMBL
Match: A0A6J1KCG9 (4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493060 PE=4 SV=1)

HSP 1 Score: 962 bits (2488), Expect = 0.0
Identity = 488/546 (89.38%), Postives = 513/546 (93.96%), Query Frame = 0


Query: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60
           MA E + +D IFRSKLPDIYIP HLPLHSYCL E +A+IGHRTCLIN+VTGESFTY DVD
Sbjct: 1   MANETV-HDIIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHRTCLINAVTGESFTYSDVD 60

Query: 61  LAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           LAARK ASGL KLGIA+GDVIMLLL NSPEFVFAFLGASY GAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180
           AKGS AKL +TQSSYYEKVKEITE+LPD KIMTVDSPP GCLSFADLIQ  + E+P VEI
Sbjct: 121 AKGSKAKLTITQSSYYEKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEI 180

Query: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240
           +PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDGENPNLYYRN+DVILCVLPLFHI
Sbjct: 181 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAG TILIMPKFEIGSLLQLV+K++VSIAPIVPPIVLAIAKSPDL+KYD
Sbjct: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIR+IK GGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAE+KIVDTENGGSLPRNTPGEICI+GDQIMKGYLNNPEATAATID +GWLHT
Sbjct: 361 ACGTVVRNAEIKIVDTENGGSLPRNTPGEICIKGDQIMKGYLNNPEATAATIDNEGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480
           GDIG IDDDDELFIVDRLKELIKYKGFQVAPAE+EALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540
           PVAFVV+LKNS+VTEDEIKQFISKQVVFYKRI R F I AIPKSPSGKILRKELRAKLA 
Sbjct: 481 PVAFVVKLKNSKVTEDEIKQFISKQVVFYKRINRVFFIGAIPKSPSGKILRKELRAKLAA 540

Query: 541 GFPNSN 546
            FP  N
Sbjct: 541 DFPIPN 545

[Close]

BLAST of Csor.00g186560 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 763.5 bits (1970), Expect = 1.2e-220
Identity = 378/541 (69.87%), Postives = 454/541 (83.92%), Query Frame = 0


Query: 8   NDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVDLAARKVA 67
           ND IFRS+LPDIYIPNHLPLH Y + EN+++   + CLIN  TGE +TY DV + +RK+A
Sbjct: 19  NDVIFRSRLPDIYIPNHLPLHDY-IFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 78

Query: 68  SGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSNAK 127
           +GL+ LG+ Q DV+M+LL NSPE V  FL AS+ GAI T+ANPFFT AEI+KQAK S AK
Sbjct: 79  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 138

Query: 128 LIVTQSSYYEKVKEITEELPDVKIMTVDSP--PDGCLSFADLIQADEREMPTV--EINPD 187
           LIVTQS Y +K+K +  +   V I+T DS   P+ CL F++L Q++E  + ++  +I+P+
Sbjct: 139 LIVTQSRYVDKIKNLQND--GVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPE 198

Query: 188 DVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHIYSL 247
           DVVALP+SSGTTGLPKGVMLTH+GLVTSVAQQVDGENPNLY+  +DVILCVLP+FHIY+L
Sbjct: 199 DVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYAL 258

Query: 248 NSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYDLSS 307
           NS++LC LR G TILIMPKFEI  LL+ +Q+ KV++A +VPPIVLAIAKSP+ +KYDLSS
Sbjct: 259 NSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSS 318

Query: 308 IRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACG 367
           +R++K G APLGKELED + AKFP A LGQGYGMTEAGPVL M L FAKEPFP+K GACG
Sbjct: 319 VRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACG 378

Query: 368 TVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDI 427
           TVVRNAEMKI+D + G SLPRN PGEICIRG+QIMKGYLN+P ATA+TIDKDGWLHTGD+
Sbjct: 379 TVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDV 438

Query: 428 GFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEVPVA 487
           GFIDDDDELFIVDRLKELIKYKGFQVAPAE+E+LL+ HP I+D AVV MK+E+AGEVPVA
Sbjct: 439 GFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVA 498

Query: 488 FVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLATGFP 545
           FVVR K+S ++EDEIKQF+SKQVVFYKRI + F  D+IPK+PSGKILRK+LRA+LA G  
Sbjct: 499 FVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLANGLM 556

[Close]

BLAST of Csor.00g186560 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 756.5 bits (1952), Expect = 1.5e-218
Identity = 378/542 (69.74%), Postives = 446/542 (82.29%), Query Frame = 0


Query: 8   NDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVDLAARKVA 67
           +D IFRSKLPDIYIPNHL LH Y + +N+++   + CLIN  TG  +TY DV + +R++A
Sbjct: 21  SDVIFRSKLPDIYIPNHLSLHDY-IFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIA 80

Query: 68  SGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSNAK 127
           +  +KLG+ Q DV+MLLL N PEFV +FL AS+RGA  TAANPFFT AEIAKQAK SN K
Sbjct: 81  ANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTK 140

Query: 128 LIVTQSSYYEKVKEITEELPDVKIMTVDSP-----PDGCLSFADLIQAD---EREMPTVE 187
           LI+T++ Y +K+K +  +   V I+ +D       P+GCL F +L Q+       + +VE
Sbjct: 141 LIITEARYVDKIKPLQND-DGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVE 200

Query: 188 INPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFH 247
           I+PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDGENPNLY+ ++DVILCVLP+FH
Sbjct: 201 ISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFH 260

Query: 248 IYSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKY 307
           IY+LNS++LCGLR G  ILIMPKFEI  LL+L+Q+ KV++AP+VPPIVLAIAKS + +KY
Sbjct: 261 IYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKY 320

Query: 308 DLSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKP 367
           DLSSIRV+K G APLGKELED V AKFP A LGQGYGMTEAGPVL M L FAKEPFP+K 
Sbjct: 321 DLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKS 380

Query: 368 GACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLH 427
           GACGTVVRNAEMKIVD + G SL RN PGEICIRG QIMKGYLNNP ATA TIDKDGWLH
Sbjct: 381 GACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLH 440

Query: 428 TGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGE 487
           TGDIG IDDDDELFIVDRLKELIKYKGFQVAPAE+EALL+ HP I+D AVV MK+E AGE
Sbjct: 441 TGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGE 500

Query: 488 VPVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLA 542
           VPVAFVV+ K+SE++ED++KQF+SKQVVFYKRI + F  ++IPK+PSGKILRK+LRAKLA
Sbjct: 501 VPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKLA 560

[Close]

BLAST of Csor.00g186560 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 705.7 bits (1820), Expect = 2.9e-203
Identity = 353/507 (69.63%), Postives = 416/507 (82.05%), Query Frame = 0


Query: 8   NDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVDLAARKVA 67
           +D IFRSKLPDIYIPNHL LH Y + +N+++   + CLIN  TG  +TY DV + +R++A
Sbjct: 21  SDVIFRSKLPDIYIPNHLSLHDY-IFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIA 80

Query: 68  SGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSNAK 127
           +  +KLG+ Q DV+MLLL N PEFV +FL AS+RGA  TAANPFFT AEIAKQAK SN K
Sbjct: 81  ANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTK 140

Query: 128 LIVTQSSYYEKVKEITEELPDVKIMTVDSP-----PDGCLSFADLIQAD---EREMPTVE 187
           LI+T++ Y +K+K +  +   V I+ +D       P+GCL F +L Q+       + +VE
Sbjct: 141 LIITEARYVDKIKPLQND-DGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVE 200

Query: 188 INPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFH 247
           I+PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDGENPNLY+ ++DVILCVLP+FH
Sbjct: 201 ISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFH 260

Query: 248 IYSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKY 307
           IY+LNS++LCGLR G  ILIMPKFEI  LL+L+Q+ KV++AP+VPPIVLAIAKS + +KY
Sbjct: 261 IYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKY 320

Query: 308 DLSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKP 367
           DLSSIRV+K G APLGKELED V AKFP A LGQGYGMTEAGPVL M L FAKEPFP+K 
Sbjct: 321 DLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKS 380

Query: 368 GACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLH 427
           GACGTVVRNAEMKIVD + G SL RN PGEICIRG QIMKGYLNNP ATA TIDKDGWLH
Sbjct: 381 GACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLH 440

Query: 428 TGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGE 487
           TGDIG IDDDDELFIVDRLKELIKYKGFQVAPAE+EALL+ HP I+D AVV MK+E AGE
Sbjct: 441 TGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGE 500

Query: 488 VPVAFVVRLKNSEVTEDEIKQFISKQV 507
           VPVAFVV+ K+SE++ED++KQF+SKQV
Sbjct: 501 VPVAFVVKSKDSELSEDDVKQFVSKQV 525

[Close]

BLAST of Csor.00g186560 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 689.9 bits (1779), Expect = 1.7e-198
Identity = 339/531 (63.84%), Postives = 429/531 (80.79%), Query Frame = 0


Query: 11  IFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVDLAARKVASGL 70
           IFRSKLPDI IPNHLPLH+YC  E L+ +  + CLI   TG+S+TY +  L  R+VASGL
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCF-EKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGL 93

Query: 71  NKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSNAKLIV 130
            KLGI +GDVIM+LLQNS EFVF+F+GAS  GA+ T ANPF+T+ E+ KQ K S AKLI+
Sbjct: 94  YKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLII 153

Query: 131 TQSSYYEKVKEITEELPDVKIMTVDSP-PDGCLSFADLIQADERE--MPTVEINPDDVVA 190
           T S Y +K+K + E L    ++T D P P+ CL F+ LI  DE      TV+I  DD  A
Sbjct: 154 THSQYVDKLKNLGENL---TLITTDEPTPENCLPFSTLITDDETNPFQETVDIGGDDAAA 213

Query: 191 LPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHIYSLNSVL 250
           LP+SSGTTGLPKGV+LTH+ L+TSVAQQVDG+NPNLY ++ DVILCVLPLFHIYSLNSVL
Sbjct: 214 LPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVL 273

Query: 251 LCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYDLSSIRVI 310
           L  LR+G T+L+M KFEIG+LL L+Q+ +V+IA +VPP+V+A+AK+P ++ YDLSS+R +
Sbjct: 274 LNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFV 333

Query: 311 KCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVR 370
             G APLGKEL+D++R + P+A+LGQGYGMTEAGPVL+M L FAKEP P K G+CGTVVR
Sbjct: 334 LSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVR 393

Query: 371 NAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFID 430
           NAE+K+V  E   SL  N PGEICIRG QIMK YLN+PEAT+ATID++GWLHTGDIG++D
Sbjct: 394 NAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVD 453

Query: 431 DDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEVPVAFVVR 490
           +DDE+FIVDRLKE+IK+KGFQV PAE+E+LL+ H +I+DAAVVP  DE AGEVPVAFVVR
Sbjct: 454 EDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVR 513

Query: 491 LKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKL 539
              +++TE+++K++++KQVVFYKR+ + F + +IPKSPSGKILRK+L+AKL
Sbjct: 514 SNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

[Close]

BLAST of Csor.00g186560 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 668.7 bits (1724), Expect = 4.0e-192
Identity = 330/550 (60.00%), Postives = 422/550 (76.73%), Query Frame = 0


Query: 4   EALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIG----HRTCLINSVTGESFTYHDV 63
           E   +DFIFRSKLPDI+IPNHLPL  Y + +  +  G      TC+I+  TG   TY DV
Sbjct: 21  EEPSHDFIFRSKLPDIFIPNHLPLTDY-VFQRFSGDGDGDSSTTCIIDGATGRILTYADV 80

Query: 64  DLAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAK 123
               R++A+G+++LGI  GDV+MLLL NSPEF  +FL  +Y GA+ T ANPF+T  EIAK
Sbjct: 81  QTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAK 140

Query: 124 QAKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVD---------SPPDGCLSFADLIQA 183
           QAK S AK+I+T+    +K+  +  +   V I+ +D         S  DGC+SF +L QA
Sbjct: 141 QAKASAAKMIITKKCLVDKLTNLKND--GVLIVCLDDDGDNGVVSSSDDGCVSFTELTQA 200

Query: 184 DEREMPTVEINPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDV 243
           DE E+   +I+P+D VA+PYSSGTTGLPKGVM+TH+GLVTS+AQ+VDGENPNL +   DV
Sbjct: 201 DETELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDV 260

Query: 244 ILCVLPLFHIYSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAI 303
           ILC LP+FHIY+L++++L  +R G  +LI+P+FE+  +++L+Q++KV++ P+ PP+VLA 
Sbjct: 261 ILCFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAF 320

Query: 304 AKSPDLDKYDLSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAF 363
            KSP+ ++YDLSS+R++  G A L KELED VR KFP A+ GQGYGMTE+G V    LAF
Sbjct: 321 IKSPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAF 380

Query: 364 AKEPFPMKPGACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAA 423
           AK PF  K GACGTV+RNAEMK+VDTE G SLPRN  GEIC+RG Q+MKGYLN+PEATA 
Sbjct: 381 AKNPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATAR 440

Query: 424 TIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVV 483
           TIDKDGWLHTGDIGF+DDDDE+FIVDRLKELIK+KG+QVAPAE+EALL++HP+I DAAVV
Sbjct: 441 TIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVV 500

Query: 484 PMKDEEAGEVPVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKIL 541
            MKDE A EVPVAFV R + S++TED++K +++KQVV YKRIK  F I+ IPK+ SGKIL
Sbjct: 501 AMKDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKIL 560

The following BLAST results are available for this feature:

BLAST of Csor.00g186560 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O24146	5.9e-233	74.82	4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1	[more]
P14912	5.0e-232	74.49	4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1	[more]
O24145	8.6e-232	74.07	4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1	[more]
P14913	1.9e-231	74.49	4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1	[more]
M4ISH0	6.1e-230	74.07	4-coumarate--CoA ligase CCL1 OS=Humulus lupulus OX=3486 GN=CCL1 PE=1 SV=1	[more]

BLAST of Csor.00g186560 vs. NCBI nr


Match Name	Identity	Description
KAG6581611.1	100.00	4-coumarate--CoA ligase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022935389.1	99.63	4-coumarate--CoA ligase 1-like [Cucurbita moschata]	[more]
XP_023526871.1	99.45	4-coumarate--CoA ligase 1-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022983944.1	98.72	4-coumarate--CoA ligase 1-like [Cucurbita maxima]	[more]
XP_023526872.1	99.41	4-coumarate--CoA ligase 1-like isoform X2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Csor.00g186560 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1F599	99.63	4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111442288 PE=...	[more]
A0A6J1J912	98.72	4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482411 PE=4 ...	[more]
A0A5A7SWF8	89.74	4-coumarate--CoA ligase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A6J1G9T7	90.06	4-coumarate--CoA ligase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1KCG9	89.38	4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493060 PE=4 ...	[more]

BLAST of Csor.00g186560 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G21240.1	1.2e-220	69.87	4-coumarate:CoA ligase 2	[more]
AT1G51680.1	1.5e-218	69.74	4-coumarate:CoA ligase 1	[more]
AT1G51680.3	2.9e-203	69.63	4-coumarate:CoA ligase 1	[more]
AT1G65060.1	1.7e-198	63.84	4-coumarate:CoA ligase 3	[more]
AT3G21230.1	4.0e-192	60.00	4-coumarate:CoA ligase 5	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR025110	AMP-binding enzyme, C-terminal domain	PFAM	PF13193	AMP-binding_C	coord: 453..528 e-value: 2.6E-16 score: 60.3
None	No IPR available	GENE3D	3.30.300.30		coord: 439..540 e-value: 1.1E-32 score: 114.8
None	No IPR available	PANTHER	PTHR24096:SF382	4-COUMARATE--COA LIGASE 2-LIKE	coord: 6..540
None	No IPR available	PANTHER	PTHR24096	LONG-CHAIN-FATTY-ACID--COA LIGASE	coord: 6..540
None	No IPR available	CDD	cd05904	4CL	coord: 21..534 e-value: 0.0 score: 769.864
None	No IPR available	SUPERFAMILY	56801	Acetyl-CoA synthetase-like	coord: 17..540
IPR000873	AMP-dependent synthetase/ligase	PFAM	PF00501	AMP-binding	coord: 35..444 e-value: 4.8E-111 score: 371.4
IPR042099	ANL, N-terminal domain	GENE3D	3.40.50.12780		coord: 5..438 e-value: 1.3E-171 score: 573.0
IPR020845	AMP-binding, conserved site	PROSITE	PS00455	AMP_BINDING	coord: 188..199

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g186560.m01	Csor.00g186560.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016874	ligase activity