Csor.00g186100 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g186100
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionphospholipase A1-Ialpha2, chloroplastic-like
LocationCsor_Chr14: 13685032 .. 13686908 (-)
RNA-Seq ExpressionCsor.00g186100
SyntenyCsor.00g186100
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintronterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTACAACAACTTTGAAGCTCAATCTTAATATCAACCATGTCACATTTTCCGAGACAACTCGACCCGTGTCTCATTCGTTTGGACAAGTTTCACTACCGAGAAAATCAGATCATGTTTCGTCCACGTCTCGACGTTCGGTTTCTTCTTCTTCTTCCTCTTCGGTTTTAGTACCGTTGATTGATATGTCTTCTTCTTCTTCTTCTTCTACCTCGGCCGCTGTTGCCATGGTTCATAAGGTGGCCGGTTTGGCACGCTTGTGGCGACAAATTCATGGCTGCAATGATTGGGAACATCTCGTCGAACCGACACTTCATCCGCTTCTTCGACGTGAAGTTATTCGGTATGGTGAGTTTGTGACGGCTTGTTATAAAGCTTTTGATCTTGATCCCAACTCCAAACGGTACTTGACTTGCAAATTTGGGAAGAAGAGTTTGTTGAAGGAAGTGGGGTTGGAGAGTTCAGGGTATGAGGTGACTAAATATATATATGCTACCCCACCGGATATCAATATTCCTCCTATTCAAAACTCGCCTCCGTCTTGTGGCCGGTGGATCGGGTATGTCGCTGTCTCCTCCGATGAAACTAGCGAGAGACTAGGGCGTCGAGATGTTGTCATCACTTTCCGTGGTACCGTCACGAATTCTGAGTGGATTGCTAATCTCATGAGCTCGCTTACTCCTGCCCGCCTCGATCCCCATAATCATCGGCCTGATGTCAAGGTTTGTTAATCTAATCATTCTATCCAAAAGTTCTAATTTATTACTCTTATCCTACATCAGTTAGAGAGGGGAACGAAGCATTCTTTATACGGGTGTGGAAACTTCTCTTTAGCAAACGCATTTTAAAACCCTTGAAGGGAAGCCTAGAAGAAAAAACCTAAAAAGAACAATATCTGCTAGCGGTGGGTTTGGGCGGTTACAAATGGTATCAGAGCCAGTCACCGAGCGGTGTGCCTATGAGGACTCTGGCCCTCGAGAGGAAAATGAATCTTTCCTTATAAGGGTGTGAAAACCTCTCCCTAGCAAACACGTTTTAAAAACCTTGAGGGAAAACCCATCAGTAAAAAACCCAAACACAACAAAATCTACTAGCACTGAGTTTGGGCTATTACGGTTACCCTCTTTTACCACAATTTAAAGATCAACCCAAGACCCAAAAACGAACGATTTGCTCTTAATATGAAATTAACTTCTCAAAACCTAATATCTTACGATAAATTCTTACCTTTTTTCAGGTCGAATCAGGGTTTTTAACACTCTACACATGCGAAGAAACTAGCACGAAGTTCGGCCTAGAGAGTTGCCGAGAGCAGCTCCTCTCGGAGATCTCGAGGCTACTAAACAAGTACAAAAGTGAAGAAGTAAGCATAACCATGGCAGGGCATAGCATGGGGAGTGCATTAGCTCTTCTCTTAGCATATGACATAGCCGAGCTAGGGTTAAACAAAAGAACAAACAAGGAAGTGGTACCCGTTTCAGTGTTCTCCTTTGGAGGTCCAAGAGTTGGTAACTCAAGATTCAAACAAAGATGTGAAGAATTAGGAGTCAAAGTATTGAGAGTTGTGAATGTGAACGATCCCATCACAAAAATGCCAGGCGTTTTTGGAGGCTATGAACATGTTGGTGTTGAATTGGTTCTTGATTTCTTCAACATGCAAAACCCTTCATGTGTTCATGATTTGGAGACTTACATAAGCTTGCTAAGATACCCAAAAGAAGAACATCAAGAACATGATGATGATCATCATCATCATAATGGAAGAAGAAAAGCTATTAATGGTGGTGATTTCATCAACAAAGCTATGGAGTTTTTGAGCTTCAACATGTTTCCATGGCGGAATCCCATGAATTATTTGAGTCAATCACAAAATTAG

mRNA sequence

ATGGCTACAACAACTTTGAAGCTCAATCTTAATATCAACCATGTCACATTTTCCGAGACAACTCGACCCGTGTCTCATTCGTTTGGACAAGTTTCACTACCGAGAAAATCAGATCATGTTTCGTCCACGTCTCGACGTTCGGTTTCTTCTTCTTCTTCCTCTTCGGTTTTAGTACCGTTGATTGATATGTCTTCTTCTTCTTCTTCTTCTACCTCGGCCGCTGTTGCCATGGTTCATAAGGTGGCCGGTTTGGCACGCTTGTGGCGACAAATTCATGGCTGCAATGATTGGGAACATCTCGTCGAACCGACACTTCATCCGCTTCTTCGACGTGAAGTTATTCGGTATGGTGAGTTTGTGACGGCTTGTTATAAAGCTTTTGATCTTGATCCCAACTCCAAACGGTACTTGACTTGCAAATTTGGGAAGAAGAGTTTGTTGAAGGAAGTGGGGTTGGAGAGTTCAGGGTATGAGGTGACTAAATATATATATGCTACCCCACCGGATATCAATATTCCTCCTATTCAAAACTCGCCTCCGTCTTGTGGCCGGTGGATCGGGTATGTCGCTGTCTCCTCCGATGAAACTAGCGAGAGACTAGGGCGTCGAGATGTTGTCATCACTTTCCGTGGTACCGTCACGAATTCTGAGTGGATTGCTAATCTCATGAGCTCGCTTACTCCTGCCCGCCTCGATCCCCATAATCATCGGCCTGATGTCAAGGTCGAATCAGGGTTTTTAACACTCTACACATGCGAAGAAACTAGCACGAAGTTCGGCCTAGAGAGTTGCCGAGAGCAGCTCCTCTCGGAGATCTCGAGGCTACTAAACAAGTACAAAAGTGAAGAAGTAAGCATAACCATGGCAGGGCATAGCATGGGGAGTGCATTAGCTCTTCTCTTAGCATATGACATAGCCGAGCTAGGGTTAAACAAAAGAACAAACAAGGAAGTGGTACCCGTTTCAGTGTTCTCCTTTGGAGGTCCAAGAGTTGGTAACTCAAGATTCAAACAAAGATGTGAAGAATTAGGAGTCAAAGTATTGAGAGTTGTGAATGTGAACGATCCCATCACAAAAATGCCAGGCGTTTTTGGAGGCTATGAACATGTTGGTGTTGAATTGGTTCTTGATTTCTTCAACATGCAAAACCCTTCATGTGTTCATGATTTGGAGACTTACATAAGCTTGCTAAGATACCCAAAAGAAGAACATCAAGAACATGATGATGATCATCATCATCATAATGGAAGAAGAAAAGCTATTAATGGTGGTGATTTCATCAACAAAGCTATGGAGTTTTTGAGCTTCAACATGTTTCCATGGCGGAATCCCATGAATTATTTGAGTCAATCACAAAATTAG

Coding sequence (CDS)

ATGGCTACAACAACTTTGAAGCTCAATCTTAATATCAACCATGTCACATTTTCCGAGACAACTCGACCCGTGTCTCATTCGTTTGGACAAGTTTCACTACCGAGAAAATCAGATCATGTTTCGTCCACGTCTCGACGTTCGGTTTCTTCTTCTTCTTCCTCTTCGGTTTTAGTACCGTTGATTGATATGTCTTCTTCTTCTTCTTCTTCTACCTCGGCCGCTGTTGCCATGGTTCATAAGGTGGCCGGTTTGGCACGCTTGTGGCGACAAATTCATGGCTGCAATGATTGGGAACATCTCGTCGAACCGACACTTCATCCGCTTCTTCGACGTGAAGTTATTCGGTATGGTGAGTTTGTGACGGCTTGTTATAAAGCTTTTGATCTTGATCCCAACTCCAAACGGTACTTGACTTGCAAATTTGGGAAGAAGAGTTTGTTGAAGGAAGTGGGGTTGGAGAGTTCAGGGTATGAGGTGACTAAATATATATATGCTACCCCACCGGATATCAATATTCCTCCTATTCAAAACTCGCCTCCGTCTTGTGGCCGGTGGATCGGGTATGTCGCTGTCTCCTCCGATGAAACTAGCGAGAGACTAGGGCGTCGAGATGTTGTCATCACTTTCCGTGGTACCGTCACGAATTCTGAGTGGATTGCTAATCTCATGAGCTCGCTTACTCCTGCCCGCCTCGATCCCCATAATCATCGGCCTGATGTCAAGGTCGAATCAGGGTTTTTAACACTCTACACATGCGAAGAAACTAGCACGAAGTTCGGCCTAGAGAGTTGCCGAGAGCAGCTCCTCTCGGAGATCTCGAGGCTACTAAACAAGTACAAAAGTGAAGAAGTAAGCATAACCATGGCAGGGCATAGCATGGGGAGTGCATTAGCTCTTCTCTTAGCATATGACATAGCCGAGCTAGGGTTAAACAAAAGAACAAACAAGGAAGTGGTACCCGTTTCAGTGTTCTCCTTTGGAGGTCCAAGAGTTGGTAACTCAAGATTCAAACAAAGATGTGAAGAATTAGGAGTCAAAGTATTGAGAGTTGTGAATGTGAACGATCCCATCACAAAAATGCCAGGCGTTTTTGGAGGCTATGAACATGTTGGTGTTGAATTGGTTCTTGATTTCTTCAACATGCAAAACCCTTCATGTGTTCATGATTTGGAGACTTACATAAGCTTGCTAAGATACCCAAAAGAAGAACATCAAGAACATGATGATGATCATCATCATCATAATGGAAGAAGAAAAGCTATTAATGGTGGTGATTTCATCAACAAAGCTATGGAGTTTTTGAGCTTCAACATGTTTCCATGGCGGAATCCCATGAATTATTTGAGTCAATCACAAAATTAG

Protein sequence

MATTTLKLNLNINHVTFSETTRPVSHSFGQVSLPRKSDHVSSTSRRSVSSSSSSSVLVPLIDMSSSSSSSTSAAVAMVHKVAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNSPPSCGRWIGYVAVSSDETSERLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMGSALALLLAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDPITKMPGVFGGYEHVGVELVLDFFNMQNPSCVHDLETYISLLRYPKEEHQEHDDDHHHHNGRRKAINGGDFINKAMEFLSFNMFPWRNPMNYLSQSQN
Homology
BLAST of Csor.00g186100 vs. ExPASy Swiss-Prot
Match: Q9SIN9 (Phospholipase A1-Ialpha2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL5 PE=1 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 2.7e-130
Identity = 259/475 (54.53%), Postives = 321/475 (67.58%), Query Frame = 0

Query: 9   NLNINHVTFSETTRPVSHSFGQVSLPRKSDHVSSTSRRSVSSSSSSSVLVPLIDMSSSSS 68
           N N+ H  F     P    F   +L     + +    ++ +SSSSSS+L P+I ++S  +
Sbjct: 6   NPNMKHAPFLRNRSPQQTLFIPYTLSLPISYQNPKRLKTANSSSSSSLLAPVI-LNSPVA 65

Query: 69  SSTSAAVAMVHK--------VAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIRYGEFV 128
           SS+   +    K           L+R+WR+I GCN+W+ L+EP L+PLL++E+ RYG  V
Sbjct: 66  SSSPPPIYCAPKFPCSSGAATVPLSRVWREIQGCNNWKDLIEP-LNPLLQQEITRYGNLV 125

Query: 129 TACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLES-SGYEVTKYIYATPP-DINIPPIQNS 188
           + CYKAFDLDPNSKRYL CK+GK++LLKE  ++    Y+VTKYIYATP  +INI PIQN 
Sbjct: 126 STCYKAFDLDPNSKRYLNCKYGKQTLLKETEIDQPEDYQVTKYIYATPDININISPIQNE 185

Query: 189 PPSCGRWIGYVAVSSDETSERLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHNHRP 248
                RW+GYVA SSD++ +RLGRRD+V+TFRGTVTN EW+AN MSSLTPAR  PHN R 
Sbjct: 186 MNRRARWVGYVAASSDDSVKRLGRRDIVVTFRGTVTNPEWLANFMSSLTPARFHPHNPRL 245

Query: 249 DVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMGSALA 308
           DVKVESGFL+LYT +E+ +KFGLESCR+QLLSEISRL+NKYK EE+SIT+AGHSMGS+LA
Sbjct: 246 DVKVESGFLSLYTSDESESKFGLESCRQQLLSEISRLMNKYKGEEMSITLAGHSMGSSLA 305

Query: 309 LLLAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDPIT 368
            LLAYDIAELGLN+R  K  +PV+VFSF GPRVGN  FK+RCEELGVKVLR+ NVNDP+T
Sbjct: 306 QLLAYDIAELGLNRRIGKGDIPVTVFSFAGPRVGNLEFKKRCEELGVKVLRITNVNDPVT 365

Query: 369 KMPG--------VFGG----------YEHVGVELVLDFFNMQNPSCVHDLETYISLLRYP 428
           K+PG        V GG          Y HVGVEL LDFF++QN SCVHDL+TYI LL   
Sbjct: 366 KLPGVLFNENFRVLGGFYELPWSCSCYVHVGVELTLDFFDVQNISCVHDLQTYIDLLNQR 425

Query: 429 KEEHQEHDDDHHHHNGRRKAINGGDFINKAMEFLSFN----MFPWRNPMNYLSQS 452
           +   +  D D              D  N A+EFL  N    MF  R  M Y S +
Sbjct: 426 RTNSRSVDSDEDE-----------DSDNVALEFLKTNGEKMMFLKRQRMMYWSNA 467

BLAST of Csor.00g186100 vs. ExPASy Swiss-Prot
Match: Q9MA46 (Galactolipase DONGLE, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DGL PE=1 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 1.0e-129
Identity = 253/424 (59.67%), Postives = 315/424 (74.29%), Query Frame = 0

Query: 38  DHVS---STSRRSVSSSSSSSVLVPLIDMSSSSSSSTSA-----AVAMVHKVAGLARLWR 97
           DH S    TS+R V SSS+ S  +    +S  SSSS+ A     A A+      L+R+WR
Sbjct: 29  DHFSISQHTSKRLVVSSSTMSPPISSSPLSLPSSSSSQAIPPSRAPAV---TLPLSRVWR 88

Query: 98  QIHGCNDWEHLVEPTLHPLLRREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKE 157
           +I G N+WE+L+EP L P+L++E+ RYG  ++A YK FDL+PNSKRYL+CK+GKK+LLKE
Sbjct: 89  EIQGSNNWENLIEP-LSPILQQEITRYGNLLSASYKGFDLNPNSKRYLSCKYGKKNLLKE 148

Query: 158 VGL-ESSGYEVTKYIYATPPDINIPPIQNSPPSCGRWIGYVAVSSDETSERLGRRDVVIT 217
            G+ +  GY+VTKYIYAT PDIN+ PI+N  P+  RWIGYVAVSSDE+ +RLGRRD+++T
Sbjct: 149 SGIHDPDGYQVTKYIYAT-PDINLNPIKNE-PNRARWIGYVAVSSDESVKRLGRRDILVT 208

Query: 218 FRGTVTNSEWIANLMSSLTPARLDPHNHRPDVKVESGFLTLYTCEETSTKFGLESCREQL 277
           FRGTVTN EW+ANL SSLTPARLDPHN RPDVKVESGFL LYT  E+ +KFGLESCREQL
Sbjct: 209 FRGTVTNHEWLANLKSSLTPARLDPHNPRPDVKVESGFLGLYTSGESESKFGLESCREQL 268

Query: 278 LSEISRLLNKYKSEEVSITMAGHSMGSALALLLAYDIAELGLNKRTNKEVVPVSVFSFGG 337
           LSEISRL+NK+K EE+SIT+AGHSMGS+LA LLAYDIAELG+N+R +++ VPV+VFSF G
Sbjct: 269 LSEISRLMNKHKGEEISITLAGHSMGSSLAQLLAYDIAELGMNQRRDEKPVPVTVFSFAG 328

Query: 338 PRVGNSRFKQRCEELGVKVLRVVNVNDPITKMPGV--------FGG----------YEHV 397
           PRVGN  FK+RCEELGVKVLR+ NVNDPITK+PG          GG          Y HV
Sbjct: 329 PRVGNLGFKKRCEELGVKVLRITNVNDPITKLPGFLFNENFRSLGGVYELPWSCSCYTHV 388

Query: 398 GVELVLDFFNMQNPSCVHDLETYISLLRYPKEEHQEHDDDHHHHNGRRKAINGGDFINKA 435
           GVEL LDFF++QN SCVHDLETYI+L+  P+      ++D+           GG+F+N+ 
Sbjct: 389 GVELTLDFFDVQNISCVHDLETYITLVNRPRCSKLAVNEDNF----------GGEFLNRT 436

BLAST of Csor.00g186100 vs. ExPASy Swiss-Prot
Match: A2WY00 (Phospholipase A1 EG1, chloroplastic/mitochondrial OS=Oryza sativa subsp. indica OX=39946 GN=EG1 PE=3 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 1.3e-113
Identity = 205/332 (61.75%), Postives = 252/332 (75.90%), Query Frame = 0

Query: 84  LARLWRQIHGCNDWEHLVEPTLHPLLRREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGK 143
           +A +WR++ GC DWE ++EP  HP+LR EV RYGE V ACYKAFDLDP S+RYL CK+G+
Sbjct: 56  VAGMWREVQGCGDWEGMLEPAPHPVLRGEVARYGELVGACYKAFDLDPASRRYLNCKYGR 115

Query: 144 KSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNSPPSCGRWIGYVAVSSDETSERLGRR 203
           + +L+EVG+  +GYEVT+YIYA   D+++P ++ S    GRWIGYVAVS+DE S RLGRR
Sbjct: 116 ERMLEEVGMGGAGYEVTRYIYAA-ADVSVPTMEPSTSGRGRWIGYVAVSTDEMSRRLGRR 175

Query: 204 DVVITFRGTVTNSEWIANLMSSLTPARLDPHNHRPDVKVESGFLTLYTCEETSTKF-GLE 263
           DV+++FRGTVT +EW+ANLMSSL  ARLDP + RPDVKVESGFL+LYT  + + +F G  
Sbjct: 176 DVLVSFRGTVTPAEWMANLMSSLEAARLDPCDPRPDVKVESGFLSLYTSADKTCRFGGAG 235

Query: 264 SCREQLLSEISRLLNKYK--SEEVSITMAGHSMGSALALLLAYDIAELGLNKRTNKEVVP 323
           SCREQLL E+SRL+  Y    E+VS+T+AGHSMGSALALL AYD+AELGLN+       P
Sbjct: 236 SCREQLLREVSRLVAAYSGGGEDVSVTLAGHSMGSALALLSAYDLAELGLNR-----AAP 295

Query: 324 VSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDPITKMPGVF--------------GG 383
           V+VFSFGGPRVGN+ FK RC+ELGVK LRV NV+DPITK+PGVF                
Sbjct: 296 VTVFSFGGPRVGNAAFKARCDELGVKALRVTNVHDPITKLPGVFLNEATAGVLRPWRHSC 355

Query: 384 YEHVGVELVLDFFNMQNPSCVHDLETYISLLR 399
           Y HVGVEL LDFF + + + VHDL TYISLLR
Sbjct: 356 YTHVGVELPLDFFKVGDLASVHDLATYISLLR 381

BLAST of Csor.00g186100 vs. ExPASy Swiss-Prot
Match: Q8S1D9 (Phospholipase A1 EG1, chloroplastic/mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=EG1 PE=1 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 1.3e-113
Identity = 205/332 (61.75%), Postives = 252/332 (75.90%), Query Frame = 0

Query: 84  LARLWRQIHGCNDWEHLVEPTLHPLLRREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGK 143
           +A +WR++ GC DWE ++EP  HP+LR EV RYGE V ACYKAFDLDP S+RYL CK+G+
Sbjct: 56  VAGMWREVQGCGDWEGMLEPAPHPVLRGEVARYGELVGACYKAFDLDPASRRYLNCKYGR 115

Query: 144 KSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNSPPSCGRWIGYVAVSSDETSERLGRR 203
           + +L+EVG+  +GYEVT+YIYA   D+++P ++ S    GRWIGYVAVS+DE S RLGRR
Sbjct: 116 ERMLEEVGMGGAGYEVTRYIYAA-ADVSVPTMEPSTSGRGRWIGYVAVSTDEMSRRLGRR 175

Query: 204 DVVITFRGTVTNSEWIANLMSSLTPARLDPHNHRPDVKVESGFLTLYTCEETSTKF-GLE 263
           DV+++FRGTVT +EW+ANLMSSL  ARLDP + RPDVKVESGFL+LYT  + + +F G  
Sbjct: 176 DVLVSFRGTVTPAEWMANLMSSLEAARLDPCDPRPDVKVESGFLSLYTSADKTCRFGGAG 235

Query: 264 SCREQLLSEISRLLNKYK--SEEVSITMAGHSMGSALALLLAYDIAELGLNKRTNKEVVP 323
           SCREQLL E+SRL+  Y    E+VS+T+AGHSMGSALALL AYD+AELGLN+       P
Sbjct: 236 SCREQLLREVSRLVAAYSGGGEDVSVTLAGHSMGSALALLSAYDLAELGLNR-----AAP 295

Query: 324 VSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDPITKMPGVF--------------GG 383
           V+VFSFGGPRVGN+ FK RC+ELGVK LRV NV+DPITK+PGVF                
Sbjct: 296 VTVFSFGGPRVGNAAFKARCDELGVKALRVTNVHDPITKLPGVFLNEATAGVLRPWRHSC 355

Query: 384 YEHVGVELVLDFFNMQNPSCVHDLETYISLLR 399
           Y HVGVEL LDFF + + + VHDL TYISLLR
Sbjct: 356 YTHVGVELPLDFFKVGDLASVHDLATYISLLR 381

BLAST of Csor.00g186100 vs. ExPASy Swiss-Prot
Match: Q3EBR6 (Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL3 PE=1 SV=2)

HSP 1 Score: 291.6 bits (745), Expect = 1.6e-77
Identity = 174/399 (43.61%), Postives = 233/399 (58.40%), Query Frame = 0

Query: 88  WRQIHGCNDWEHLVEPTLHPLLRREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLL 147
           WR+I G +DW  L++P + P+LR E+IRYGE   ACY AFD DP SK   T +F +    
Sbjct: 105 WRKIQGEDDWAGLMDP-MDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFF 164

Query: 148 KEVGLESSGYEVTKYIYATPPDINIPPIQNSP------PSCGRWIGYVAVSSDETS-ERL 207
             +G+  SGYEV +Y+YAT  +IN+P   +             W+GYVAVS DETS  RL
Sbjct: 165 DSLGMIDSGYEVARYLYAT-SNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSRNRL 224

Query: 208 GRRDVVITFRGTVTNSEWIANLMSSLTPARLDP-HNHRPDVKVESGFLTLYTCEETSTKF 267
           GRRD+ I +RGTVT  EWIA+L   L P   +      P VKVESGFL LYT ++T+ KF
Sbjct: 225 GRRDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPDPAVKVESGFLDLYTDKDTTCKF 284

Query: 268 GLESCREQLLSEISRLLNKYKSE---EVSITMAGHSMGSALALLLAYDIAELGLNKRTNK 327
              S REQ+L+E+ RL+ ++  +   ++SIT+ GHS+G ALA+L AYDIAE+ LN+    
Sbjct: 285 ARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSKKG 344

Query: 328 EVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDPITKMPGVFGG---------- 387
           +V+PV+V ++GGPRVGN RF++R EELGVKV+RVVNV+D + K PG+F            
Sbjct: 345 KVIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHALMKI 404

Query: 388 -------YEHVGVELVLDFFNMQ------NPSCVHDLETYISLLRYPKEEHQEHDDDHHH 447
                  Y HVG EL LD  N        + S  H+LE  + LL            D +H
Sbjct: 405 AEGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLL------------DGYH 464

BLAST of Csor.00g186100 vs. NCBI nr
Match: KAG6582093.1 (Galactolipase DONGLE, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 904 bits (2336), Expect = 0.0
Identity = 453/453 (100.00%), Postives = 453/453 (100.00%), Query Frame = 0

Query: 1   MATTTLKLNLNINHVTFSETTRPVSHSFGQVSLPRKSDHVSSTSRRSVSSSSSSSVLVPL 60
           MATTTLKLNLNINHVTFSETTRPVSHSFGQVSLPRKSDHVSSTSRRSVSSSSSSSVLVPL
Sbjct: 1   MATTTLKLNLNINHVTFSETTRPVSHSFGQVSLPRKSDHVSSTSRRSVSSSSSSSVLVPL 60

Query: 61  IDMSSSSSSSTSAAVAMVHKVAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIRYGEFV 120
           IDMSSSSSSSTSAAVAMVHKVAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIRYGEFV
Sbjct: 61  IDMSSSSSSSTSAAVAMVHKVAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIRYGEFV 120

Query: 121 TACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNSPP 180
           TACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNSPP
Sbjct: 121 TACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNSPP 180

Query: 181 SCGRWIGYVAVSSDETSERLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHNHRPDV 240
           SCGRWIGYVAVSSDETSERLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHNHRPDV
Sbjct: 181 SCGRWIGYVAVSSDETSERLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHNHRPDV 240

Query: 241 KVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMGSALALL 300
           KVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMGSALALL
Sbjct: 241 KVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMGSALALL 300

Query: 301 LAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDPITKM 360
           LAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDPITKM
Sbjct: 301 LAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDPITKM 360

Query: 361 PGVFGGYEHVGVELVLDFFNMQNPSCVHDLETYISLLRYPKEEHQEHDDDHHHHNGRRKA 420
           PGVFGGYEHVGVELVLDFFNMQNPSCVHDLETYISLLRYPKEEHQEHDDDHHHHNGRRKA
Sbjct: 361 PGVFGGYEHVGVELVLDFFNMQNPSCVHDLETYISLLRYPKEEHQEHDDDHHHHNGRRKA 420

Query: 421 INGGDFINKAMEFLSFNMFPWRNPMNYLSQSQN 453
           INGGDFINKAMEFLSFNMFPWRNPMNYLSQSQN
Sbjct: 421 INGGDFINKAMEFLSFNMFPWRNPMNYLSQSQN 453

BLAST of Csor.00g186100 vs. NCBI nr
Match: XP_022956251.1 (phospholipase A1-Ialpha2, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 894 bits (2309), Expect = 0.0
Identity = 453/458 (98.91%), Postives = 453/458 (98.91%), Query Frame = 0

Query: 1   MATTTLKLNLNINHVTFSETTRPVSHSFGQVSLPRKSDHVSSTSRRSVSSSSSSSVLVPL 60
           MATTTLKLNLNINHVTFSETTRPVSHSFGQVSLPRKSDHVSSTSRRSVSSSSSSSVLVPL
Sbjct: 1   MATTTLKLNLNINHVTFSETTRPVSHSFGQVSLPRKSDHVSSTSRRSVSSSSSSSVLVPL 60

Query: 61  IDM----SSSSSSSTSAAVAMVHKVAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIRY 120
           IDM    SSSSSSSTSAAVAMVHKVAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIRY
Sbjct: 61  IDMFSSSSSSSSSSTSAAVAMVHKVAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIRY 120

Query: 121 GEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQ 180
           GEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQ
Sbjct: 121 GEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQ 180

Query: 181 NSPPSCGRWIGYVAVSSDETSERLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHNH 240
           NSPPSCGRWIGYVAVSSDETSERLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHNH
Sbjct: 181 NSPPSCGRWIGYVAVSSDETSERLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHNH 240

Query: 241 RPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMGSA 300
           RPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMGSA
Sbjct: 241 RPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMGSA 300

Query: 301 LALLLAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDP 360
           LALLLAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDP
Sbjct: 301 LALLLAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDP 360

Query: 361 ITKMPGVFGGYEHVGVELVLDFFNMQNPSCVHDLETYISLLRYPKEEHQEHDDD-HHHHN 420
           ITKMPGVFGGYEHVGVELVLDFFNMQNPSCVHDLETYISLLRYPKEEHQEHDDD HHHHN
Sbjct: 361 ITKMPGVFGGYEHVGVELVLDFFNMQNPSCVHDLETYISLLRYPKEEHQEHDDDDHHHHN 420

Query: 421 GRRKAINGGDFINKAMEFLSFNMFPWRNPMNYLSQSQN 453
           GRRKAINGGDFINKAMEFLSFNMFPWRNPMNYLSQSQN
Sbjct: 421 GRRKAINGGDFINKAMEFLSFNMFPWRNPMNYLSQSQN 458

BLAST of Csor.00g186100 vs. NCBI nr
Match: XP_022979567.1 (phospholipase A1-Ialpha2, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 882 bits (2278), Expect = 0.0
Identity = 443/453 (97.79%), Postives = 448/453 (98.90%), Query Frame = 0

Query: 1   MATTTLKLNLNINHVTFSETTRPVSHSFGQVSLPRKSDHVSSTSRRSVSSSSSSSVLVPL 60
           MATTTLKLNLNINHVTFSETTRP+SHSFGQVSLPRKSDHVSSTSRRSVSSSSSSSVLVPL
Sbjct: 1   MATTTLKLNLNINHVTFSETTRPMSHSFGQVSLPRKSDHVSSTSRRSVSSSSSSSVLVPL 60

Query: 61  IDMSSSSSSSTSAAVAMVHKVAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIRYGEFV 120
           IDM SSSSSS SAAVAMVHK+AGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIRYGEFV
Sbjct: 61  IDMFSSSSSSASAAVAMVHKMAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIRYGEFV 120

Query: 121 TACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNSPP 180
           TACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNSPP
Sbjct: 121 TACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNSPP 180

Query: 181 SCGRWIGYVAVSSDETSERLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHNHRPDV 240
           SCGRWIGYVAVSSDETS+RLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHNHRPDV
Sbjct: 181 SCGRWIGYVAVSSDETSKRLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHNHRPDV 240

Query: 241 KVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMGSALALL 300
           KVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMGSALALL
Sbjct: 241 KVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMGSALALL 300

Query: 301 LAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDPITKM 360
           LAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDPITK+
Sbjct: 301 LAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDPITKI 360

Query: 361 PGVFGGYEHVGVELVLDFFNMQNPSCVHDLETYISLLRYPKEEHQEHDDDHHHHNGRRKA 420
           PGVFGGYEHVGVELVLDFFNMQNPSCVHDLETYISLLRYPKEEHQ+HDDDHHH NGRRK 
Sbjct: 361 PGVFGGYEHVGVELVLDFFNMQNPSCVHDLETYISLLRYPKEEHQKHDDDHHH-NGRRKV 420

Query: 421 INGGDFINKAMEFLSFNMFPWRNPMNYLSQSQN 453
           INGGDFINKAMEFLSFNM PWRNPMNYLSQSQN
Sbjct: 421 INGGDFINKAMEFLSFNMLPWRNPMNYLSQSQN 452

BLAST of Csor.00g186100 vs. NCBI nr
Match: XP_023526912.1 (galactolipase DONGLE, chloroplastic-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 800 bits (2066), Expect = 5.17e-291
Identity = 406/411 (98.78%), Postives = 408/411 (99.27%), Query Frame = 0

Query: 1   MATTTLKLNLNINHVTFSETTRPVSHSFGQVSLPRKSDHVSSTSRRSVSSSSSSSVLVPL 60
           MATTTLKLNLNINHVTFS+TTRPVSHSFGQVSLPRKSDHVSSTSRRSVSSSSSSSVLVPL
Sbjct: 1   MATTTLKLNLNINHVTFSDTTRPVSHSFGQVSLPRKSDHVSSTSRRSVSSSSSSSVLVPL 60

Query: 61  IDM--SSSSSSSTSAAVAMVHKVAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIRYGE 120
           IDM  SSSSSSSTSAAVAMVHKVAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIRYGE
Sbjct: 61  IDMFSSSSSSSSTSAAVAMVHKVAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIRYGE 120

Query: 121 FVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNS 180
           FVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNS
Sbjct: 121 FVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNS 180

Query: 181 PPSCGRWIGYVAVSSDETSERLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHNHRP 240
           PPSCGRWIGYVAVSSDETS+RLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHNHRP
Sbjct: 181 PPSCGRWIGYVAVSSDETSKRLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHNHRP 240

Query: 241 DVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMGSALA 300
           DVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMGSALA
Sbjct: 241 DVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMGSALA 300

Query: 301 LLLAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDPIT 360
           LLLAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDPIT
Sbjct: 301 LLLAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDPIT 360

Query: 361 KMPGVFGGYEHVGVELVLDFFNMQNPSCVHDLETYISLLRYPKEEHQEHDD 409
           KMPGVFGGYEHVGVEL LDFFNMQNPSCVHDLETYISLLRYPKEEHQEHDD
Sbjct: 361 KMPGVFGGYEHVGVELALDFFNMQNPSCVHDLETYISLLRYPKEEHQEHDD 411

BLAST of Csor.00g186100 vs. NCBI nr
Match: XP_004148168.2 (galactolipase DONGLE, chloroplastic [Cucumis sativus] >KGN57254.1 hypothetical protein Csa_009777 [Cucumis sativus])

HSP 1 Score: 749 bits (1935), Expect = 7.73e-270
Identity = 400/487 (82.14%), Postives = 421/487 (86.45%), Query Frame = 0

Query: 1   MATTTLKLNLNINHVTFSETTRPVSHSFGQVSLPRKSD-HVSSTSRRSVSSS-------S 60
           MA TTLKLN   NHVTF ETTRP+SHSFGQVS+PRKSD +VSS+S  S SSS       S
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDTNVSSSSSSSSSSSASRRLSVS 60

Query: 61  SSSVLVPLIDMSSSSS---SSTSAAVAMVHKVAGLARLWRQIHGCNDWEHLVEPTLHPLL 120
           SSSVLVPL+D+ SSSS    ST+AA AMV KVA LARLWRQIHGCNDWE LVEPTLHPLL
Sbjct: 61  SSSVLVPLMDVFSSSSFSSCSTAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLHPLL 120

Query: 121 RREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 180
           RRE+IRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD
Sbjct: 121 RREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 180

Query: 181 INIPPIQNSPPSCGRWIGYVAVSSDETSERLGRRDVVITFRGTVTNSEWIANLMSSLTPA 240
           INIPPIQNSPPSCGRWIGYVAVSSDETS+RLGRRD++ITFRGTVTN EWIANLMSSLTPA
Sbjct: 181 INIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTNPEWIANLMSSLTPA 240

Query: 241 RLDPHNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMA 300
           RLDPHNHRPDVKVESGFLTLYT EE+S KFGLESCREQLLSE+SRLLNKYK EEVSITMA
Sbjct: 241 RLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSEVSRLLNKYKEEEVSITMA 300

Query: 301 GHSMGSALALLLAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLR 360
           GHSMGSALALLLAYDIAELGLN+RTN EVVPVSVFSFGGPRVGNS FK+RCEELGVKVLR
Sbjct: 301 GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLR 360

Query: 361 VVNVNDPITKMPGV--------FGG---------YEHVGVELVLDFFNMQNPSCVHDLET 420
           +VNVNDPITKMPGV        FGG         YEHVGVELVLDFFNMQNPSCVHDLET
Sbjct: 361 IVNVNDPITKMPGVLFNENFRVFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHDLET 420

Query: 421 YISLLRYPKEEHQEHDDDHHHHNGRRKAI-NGGDFINKAMEFL-----SFNMFPWRNPMN 453
           YISLLR PK++  E D+D    +GR K I N G+FINKAMEFL     S NMFPWRNPMN
Sbjct: 421 YISLLRCPKKQEDE-DEDEDEDSGRGKIIINSGEFINKAMEFLCSNAQSLNMFPWRNPMN 480

BLAST of Csor.00g186100 vs. ExPASy TrEMBL
Match: A0A6J1GW19 (phospholipase A1-Ialpha2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111458003 PE=4 SV=1)

HSP 1 Score: 894 bits (2309), Expect = 0.0
Identity = 453/458 (98.91%), Postives = 453/458 (98.91%), Query Frame = 0

Query: 1   MATTTLKLNLNINHVTFSETTRPVSHSFGQVSLPRKSDHVSSTSRRSVSSSSSSSVLVPL 60
           MATTTLKLNLNINHVTFSETTRPVSHSFGQVSLPRKSDHVSSTSRRSVSSSSSSSVLVPL
Sbjct: 1   MATTTLKLNLNINHVTFSETTRPVSHSFGQVSLPRKSDHVSSTSRRSVSSSSSSSVLVPL 60

Query: 61  IDM----SSSSSSSTSAAVAMVHKVAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIRY 120
           IDM    SSSSSSSTSAAVAMVHKVAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIRY
Sbjct: 61  IDMFSSSSSSSSSSTSAAVAMVHKVAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIRY 120

Query: 121 GEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQ 180
           GEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQ
Sbjct: 121 GEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQ 180

Query: 181 NSPPSCGRWIGYVAVSSDETSERLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHNH 240
           NSPPSCGRWIGYVAVSSDETSERLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHNH
Sbjct: 181 NSPPSCGRWIGYVAVSSDETSERLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHNH 240

Query: 241 RPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMGSA 300
           RPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMGSA
Sbjct: 241 RPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMGSA 300

Query: 301 LALLLAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDP 360
           LALLLAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDP
Sbjct: 301 LALLLAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDP 360

Query: 361 ITKMPGVFGGYEHVGVELVLDFFNMQNPSCVHDLETYISLLRYPKEEHQEHDDD-HHHHN 420
           ITKMPGVFGGYEHVGVELVLDFFNMQNPSCVHDLETYISLLRYPKEEHQEHDDD HHHHN
Sbjct: 361 ITKMPGVFGGYEHVGVELVLDFFNMQNPSCVHDLETYISLLRYPKEEHQEHDDDDHHHHN 420

Query: 421 GRRKAINGGDFINKAMEFLSFNMFPWRNPMNYLSQSQN 453
           GRRKAINGGDFINKAMEFLSFNMFPWRNPMNYLSQSQN
Sbjct: 421 GRRKAINGGDFINKAMEFLSFNMFPWRNPMNYLSQSQN 458

BLAST of Csor.00g186100 vs. ExPASy TrEMBL
Match: A0A6J1IP19 (phospholipase A1-Ialpha2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111479244 PE=4 SV=1)

HSP 1 Score: 882 bits (2278), Expect = 0.0
Identity = 443/453 (97.79%), Postives = 448/453 (98.90%), Query Frame = 0

Query: 1   MATTTLKLNLNINHVTFSETTRPVSHSFGQVSLPRKSDHVSSTSRRSVSSSSSSSVLVPL 60
           MATTTLKLNLNINHVTFSETTRP+SHSFGQVSLPRKSDHVSSTSRRSVSSSSSSSVLVPL
Sbjct: 1   MATTTLKLNLNINHVTFSETTRPMSHSFGQVSLPRKSDHVSSTSRRSVSSSSSSSVLVPL 60

Query: 61  IDMSSSSSSSTSAAVAMVHKVAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIRYGEFV 120
           IDM SSSSSS SAAVAMVHK+AGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIRYGEFV
Sbjct: 61  IDMFSSSSSSASAAVAMVHKMAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIRYGEFV 120

Query: 121 TACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNSPP 180
           TACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNSPP
Sbjct: 121 TACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNSPP 180

Query: 181 SCGRWIGYVAVSSDETSERLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHNHRPDV 240
           SCGRWIGYVAVSSDETS+RLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHNHRPDV
Sbjct: 181 SCGRWIGYVAVSSDETSKRLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHNHRPDV 240

Query: 241 KVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMGSALALL 300
           KVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMGSALALL
Sbjct: 241 KVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMGSALALL 300

Query: 301 LAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDPITKM 360
           LAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDPITK+
Sbjct: 301 LAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDPITKI 360

Query: 361 PGVFGGYEHVGVELVLDFFNMQNPSCVHDLETYISLLRYPKEEHQEHDDDHHHHNGRRKA 420
           PGVFGGYEHVGVELVLDFFNMQNPSCVHDLETYISLLRYPKEEHQ+HDDDHHH NGRRK 
Sbjct: 361 PGVFGGYEHVGVELVLDFFNMQNPSCVHDLETYISLLRYPKEEHQKHDDDHHH-NGRRKV 420

Query: 421 INGGDFINKAMEFLSFNMFPWRNPMNYLSQSQN 453
           INGGDFINKAMEFLSFNM PWRNPMNYLSQSQN
Sbjct: 421 INGGDFINKAMEFLSFNMLPWRNPMNYLSQSQN 452

BLAST of Csor.00g186100 vs. ExPASy TrEMBL
Match: A0A0A0L8A7 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G174570 PE=4 SV=1)

HSP 1 Score: 749 bits (1935), Expect = 3.74e-270
Identity = 400/487 (82.14%), Postives = 421/487 (86.45%), Query Frame = 0

Query: 1   MATTTLKLNLNINHVTFSETTRPVSHSFGQVSLPRKSD-HVSSTSRRSVSSS-------S 60
           MA TTLKLN   NHVTF ETTRP+SHSFGQVS+PRKSD +VSS+S  S SSS       S
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDTNVSSSSSSSSSSSASRRLSVS 60

Query: 61  SSSVLVPLIDMSSSSS---SSTSAAVAMVHKVAGLARLWRQIHGCNDWEHLVEPTLHPLL 120
           SSSVLVPL+D+ SSSS    ST+AA AMV KVA LARLWRQIHGCNDWE LVEPTLHPLL
Sbjct: 61  SSSVLVPLMDVFSSSSFSSCSTAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLHPLL 120

Query: 121 RREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 180
           RRE+IRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD
Sbjct: 121 RREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 180

Query: 181 INIPPIQNSPPSCGRWIGYVAVSSDETSERLGRRDVVITFRGTVTNSEWIANLMSSLTPA 240
           INIPPIQNSPPSCGRWIGYVAVSSDETS+RLGRRD++ITFRGTVTN EWIANLMSSLTPA
Sbjct: 181 INIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTNPEWIANLMSSLTPA 240

Query: 241 RLDPHNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMA 300
           RLDPHNHRPDVKVESGFLTLYT EE+S KFGLESCREQLLSE+SRLLNKYK EEVSITMA
Sbjct: 241 RLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSEVSRLLNKYKEEEVSITMA 300

Query: 301 GHSMGSALALLLAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLR 360
           GHSMGSALALLLAYDIAELGLN+RTN EVVPVSVFSFGGPRVGNS FK+RCEELGVKVLR
Sbjct: 301 GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLR 360

Query: 361 VVNVNDPITKMPGV--------FGG---------YEHVGVELVLDFFNMQNPSCVHDLET 420
           +VNVNDPITKMPGV        FGG         YEHVGVELVLDFFNMQNPSCVHDLET
Sbjct: 361 IVNVNDPITKMPGVLFNENFRVFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHDLET 420

Query: 421 YISLLRYPKEEHQEHDDDHHHHNGRRKAI-NGGDFINKAMEFL-----SFNMFPWRNPMN 453
           YISLLR PK++  E D+D    +GR K I N G+FINKAMEFL     S NMFPWRNPMN
Sbjct: 421 YISLLRCPKKQEDE-DEDEDEDSGRGKIIINSGEFINKAMEFLCSNAQSLNMFPWRNPMN 480

BLAST of Csor.00g186100 vs. ExPASy TrEMBL
Match: A0A5A7VKE2 (Galactolipase DONGLE OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G00300 PE=4 SV=1)

HSP 1 Score: 748 bits (1930), Expect = 2.70e-269
Identity = 398/492 (80.89%), Postives = 423/492 (85.98%), Query Frame = 0

Query: 1   MATTTLKLNLNINHVTFSETTRPVSHSFGQVSLPRKSD-HVSSTSRRSVSSSS------- 60
           MA TTLKLN   NHVTF ETTRP+SHSFGQVS+PRKSD +VSS+S  S  SSS       
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDINVSSSSSSSSYSSSASRRLSV 60

Query: 61  -SSSVLVPLIDMSSSSSSS-----TSAAVAMVHKVAGLARLWRQIHGCNDWEHLVEPTLH 120
            SSSVLVPL+D+ SSSSSS     T+AA AMV KVA LARLWRQIHGCNDWE LVEPTLH
Sbjct: 61  SSSSVLVPLMDVFSSSSSSFSSCSTAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLH 120

Query: 121 PLLRREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT 180
           PLLRRE+IRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT
Sbjct: 121 PLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT 180

Query: 181 PPDINIPPIQNSPPSCGRWIGYVAVSSDETSERLGRRDVVITFRGTVTNSEWIANLMSSL 240
           PPDINIPPIQNSPPSCGRWIGYVAVSSDETS+RLGRRD++ITFRGTVT+ EWIANLMSSL
Sbjct: 181 PPDINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTHPEWIANLMSSL 240

Query: 241 TPARLDPHNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSI 300
           TPARLDPHNHRPDVKVESGFLTLYT EE++ KFGLESCREQLLSE+SR+LNKYK EEVSI
Sbjct: 241 TPARLDPHNHRPDVKVESGFLTLYTSEESNMKFGLESCREQLLSEVSRILNKYKEEEVSI 300

Query: 301 TMAGHSMGSALALLLAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVK 360
           TMAGHSMGSALALLLAYDIAELGLN+RTN EVVPVSVFSFGGPRVGNS FK+RC+ELGVK
Sbjct: 301 TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCDELGVK 360

Query: 361 VLRVVNVNDPITKMPGV--------FGG---------YEHVGVELVLDFFNMQNPSCVHD 420
           VLR+VNVNDPITKMPGV        FGG         YEHVGVELVLDFFNMQNPSCVHD
Sbjct: 361 VLRIVNVNDPITKMPGVLFNENFRAFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHD 420

Query: 421 LETYISLLRYPK--EEHQEHDDDHHHHNGRRK-AINGGDFINKAMEFL-----SFNMFPW 453
           LETYISLLR PK  E+  + +D+H  HNGRRK  IN G+FINKA EFL     S NMFPW
Sbjct: 421 LETYISLLRCPKQQEDRGQDEDNHGDHNGRRKITINSGEFINKAKEFLCSNAQSLNMFPW 480

BLAST of Csor.00g186100 vs. ExPASy TrEMBL
Match: A0A1S3AXV1 (galactolipase DONGLE, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103483973 PE=4 SV=1)

HSP 1 Score: 748 bits (1930), Expect = 2.70e-269
Identity = 398/492 (80.89%), Postives = 423/492 (85.98%), Query Frame = 0

Query: 1   MATTTLKLNLNINHVTFSETTRPVSHSFGQVSLPRKSD-HVSSTSRRSVSSSS------- 60
           MA TTLKLN   NHVTF ETTRP+SHSFGQVS+PRKSD +VSS+S  S  SSS       
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDINVSSSSSSSSYSSSASRRLSV 60

Query: 61  -SSSVLVPLIDMSSSSSSS-----TSAAVAMVHKVAGLARLWRQIHGCNDWEHLVEPTLH 120
            SSSVLVPL+D+ SSSSSS     T+AA AMV KVA LARLWRQIHGCNDWE LVEPTLH
Sbjct: 61  SSSSVLVPLMDVFSSSSSSFSSCSTAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLH 120

Query: 121 PLLRREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT 180
           PLLRRE+IRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT
Sbjct: 121 PLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT 180

Query: 181 PPDINIPPIQNSPPSCGRWIGYVAVSSDETSERLGRRDVVITFRGTVTNSEWIANLMSSL 240
           PPDINIPPIQNSPPSCGRWIGYVAVSSDETS+RLGRRD++ITFRGTVT+ EWIANLMSSL
Sbjct: 181 PPDINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTHPEWIANLMSSL 240

Query: 241 TPARLDPHNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSI 300
           TPARLDPHNHRPDVKVESGFLTLYT EE++ KFGLESCREQLLSE+SR+LNKYK EEVSI
Sbjct: 241 TPARLDPHNHRPDVKVESGFLTLYTSEESNMKFGLESCREQLLSEVSRILNKYKEEEVSI 300

Query: 301 TMAGHSMGSALALLLAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVK 360
           TMAGHSMGSALALLLAYDIAELGLN+RTN EVVPVSVFSFGGPRVGNS FK+RC+ELGVK
Sbjct: 301 TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCDELGVK 360

Query: 361 VLRVVNVNDPITKMPGV--------FGG---------YEHVGVELVLDFFNMQNPSCVHD 420
           VLR+VNVNDPITKMPGV        FGG         YEHVGVELVLDFFNMQNPSCVHD
Sbjct: 361 VLRIVNVNDPITKMPGVLFNENFRAFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHD 420

Query: 421 LETYISLLRYPK--EEHQEHDDDHHHHNGRRK-AINGGDFINKAMEFL-----SFNMFPW 453
           LETYISLLR PK  E+  + +D+H  HNGRRK  IN G+FINKA EFL     S NMFPW
Sbjct: 421 LETYISLLRCPKQQEDRGQDEDNHGDHNGRRKITINSGEFINKAKEFLCSNAQSLNMFPW 480

BLAST of Csor.00g186100 vs. TAIR 10
Match: AT2G31690.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 466.8 bits (1200), Expect = 1.9e-131
Identity = 259/475 (54.53%), Postives = 321/475 (67.58%), Query Frame = 0

Query: 9   NLNINHVTFSETTRPVSHSFGQVSLPRKSDHVSSTSRRSVSSSSSSSVLVPLIDMSSSSS 68
           N N+ H  F     P    F   +L     + +    ++ +SSSSSS+L P+I ++S  +
Sbjct: 6   NPNMKHAPFLRNRSPQQTLFIPYTLSLPISYQNPKRLKTANSSSSSSLLAPVI-LNSPVA 65

Query: 69  SSTSAAVAMVHK--------VAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIRYGEFV 128
           SS+   +    K           L+R+WR+I GCN+W+ L+EP L+PLL++E+ RYG  V
Sbjct: 66  SSSPPPIYCAPKFPCSSGAATVPLSRVWREIQGCNNWKDLIEP-LNPLLQQEITRYGNLV 125

Query: 129 TACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLES-SGYEVTKYIYATPP-DINIPPIQNS 188
           + CYKAFDLDPNSKRYL CK+GK++LLKE  ++    Y+VTKYIYATP  +INI PIQN 
Sbjct: 126 STCYKAFDLDPNSKRYLNCKYGKQTLLKETEIDQPEDYQVTKYIYATPDININISPIQNE 185

Query: 189 PPSCGRWIGYVAVSSDETSERLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHNHRP 248
                RW+GYVA SSD++ +RLGRRD+V+TFRGTVTN EW+AN MSSLTPAR  PHN R 
Sbjct: 186 MNRRARWVGYVAASSDDSVKRLGRRDIVVTFRGTVTNPEWLANFMSSLTPARFHPHNPRL 245

Query: 249 DVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMGSALA 308
           DVKVESGFL+LYT +E+ +KFGLESCR+QLLSEISRL+NKYK EE+SIT+AGHSMGS+LA
Sbjct: 246 DVKVESGFLSLYTSDESESKFGLESCRQQLLSEISRLMNKYKGEEMSITLAGHSMGSSLA 305

Query: 309 LLLAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDPIT 368
            LLAYDIAELGLN+R  K  +PV+VFSF GPRVGN  FK+RCEELGVKVLR+ NVNDP+T
Sbjct: 306 QLLAYDIAELGLNRRIGKGDIPVTVFSFAGPRVGNLEFKKRCEELGVKVLRITNVNDPVT 365

Query: 369 KMPG--------VFGG----------YEHVGVELVLDFFNMQNPSCVHDLETYISLLRYP 428
           K+PG        V GG          Y HVGVEL LDFF++QN SCVHDL+TYI LL   
Sbjct: 366 KLPGVLFNENFRVLGGFYELPWSCSCYVHVGVELTLDFFDVQNISCVHDLQTYIDLLNQR 425

Query: 429 KEEHQEHDDDHHHHNGRRKAINGGDFINKAMEFLSFN----MFPWRNPMNYLSQS 452
           +   +  D D              D  N A+EFL  N    MF  R  M Y S +
Sbjct: 426 RTNSRSVDSDEDE-----------DSDNVALEFLKTNGEKMMFLKRQRMMYWSNA 467

BLAST of Csor.00g186100 vs. TAIR 10
Match: AT1G05800.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 464.9 bits (1195), Expect = 7.3e-131
Identity = 253/424 (59.67%), Postives = 315/424 (74.29%), Query Frame = 0

Query: 38  DHVS---STSRRSVSSSSSSSVLVPLIDMSSSSSSSTSA-----AVAMVHKVAGLARLWR 97
           DH S    TS+R V SSS+ S  +    +S  SSSS+ A     A A+      L+R+WR
Sbjct: 29  DHFSISQHTSKRLVVSSSTMSPPISSSPLSLPSSSSSQAIPPSRAPAV---TLPLSRVWR 88

Query: 98  QIHGCNDWEHLVEPTLHPLLRREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKE 157
           +I G N+WE+L+EP L P+L++E+ RYG  ++A YK FDL+PNSKRYL+CK+GKK+LLKE
Sbjct: 89  EIQGSNNWENLIEP-LSPILQQEITRYGNLLSASYKGFDLNPNSKRYLSCKYGKKNLLKE 148

Query: 158 VGL-ESSGYEVTKYIYATPPDINIPPIQNSPPSCGRWIGYVAVSSDETSERLGRRDVVIT 217
            G+ +  GY+VTKYIYAT PDIN+ PI+N  P+  RWIGYVAVSSDE+ +RLGRRD+++T
Sbjct: 149 SGIHDPDGYQVTKYIYAT-PDINLNPIKNE-PNRARWIGYVAVSSDESVKRLGRRDILVT 208

Query: 218 FRGTVTNSEWIANLMSSLTPARLDPHNHRPDVKVESGFLTLYTCEETSTKFGLESCREQL 277
           FRGTVTN EW+ANL SSLTPARLDPHN RPDVKVESGFL LYT  E+ +KFGLESCREQL
Sbjct: 209 FRGTVTNHEWLANLKSSLTPARLDPHNPRPDVKVESGFLGLYTSGESESKFGLESCREQL 268

Query: 278 LSEISRLLNKYKSEEVSITMAGHSMGSALALLLAYDIAELGLNKRTNKEVVPVSVFSFGG 337
           LSEISRL+NK+K EE+SIT+AGHSMGS+LA LLAYDIAELG+N+R +++ VPV+VFSF G
Sbjct: 269 LSEISRLMNKHKGEEISITLAGHSMGSSLAQLLAYDIAELGMNQRRDEKPVPVTVFSFAG 328

Query: 338 PRVGNSRFKQRCEELGVKVLRVVNVNDPITKMPGV--------FGG----------YEHV 397
           PRVGN  FK+RCEELGVKVLR+ NVNDPITK+PG          GG          Y HV
Sbjct: 329 PRVGNLGFKKRCEELGVKVLRITNVNDPITKLPGFLFNENFRSLGGVYELPWSCSCYTHV 388

Query: 398 GVELVLDFFNMQNPSCVHDLETYISLLRYPKEEHQEHDDDHHHHNGRRKAINGGDFINKA 435
           GVEL LDFF++QN SCVHDLETYI+L+  P+      ++D+           GG+F+N+ 
Sbjct: 389 GVELTLDFFDVQNISCVHDLETYITLVNRPRCSKLAVNEDNF----------GGEFLNRT 436

BLAST of Csor.00g186100 vs. TAIR 10
Match: AT2G30550.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 291.6 bits (745), Expect = 1.1e-78
Identity = 174/399 (43.61%), Postives = 233/399 (58.40%), Query Frame = 0

Query: 88  WRQIHGCNDWEHLVEPTLHPLLRREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLL 147
           WR+I G +DW  L++P + P+LR E+IRYGE   ACY AFD DP SK   T +F +    
Sbjct: 105 WRKIQGEDDWAGLMDP-MDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFF 164

Query: 148 KEVGLESSGYEVTKYIYATPPDINIPPIQNSP------PSCGRWIGYVAVSSDETS-ERL 207
             +G+  SGYEV +Y+YAT  +IN+P   +             W+GYVAVS DETS  RL
Sbjct: 165 DSLGMIDSGYEVARYLYAT-SNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSRNRL 224

Query: 208 GRRDVVITFRGTVTNSEWIANLMSSLTPARLDP-HNHRPDVKVESGFLTLYTCEETSTKF 267
           GRRD+ I +RGTVT  EWIA+L   L P   +      P VKVESGFL LYT ++T+ KF
Sbjct: 225 GRRDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPDPAVKVESGFLDLYTDKDTTCKF 284

Query: 268 GLESCREQLLSEISRLLNKYKSE---EVSITMAGHSMGSALALLLAYDIAELGLNKRTNK 327
              S REQ+L+E+ RL+ ++  +   ++SIT+ GHS+G ALA+L AYDIAE+ LN+    
Sbjct: 285 ARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSKKG 344

Query: 328 EVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDPITKMPGVFGG---------- 387
           +V+PV+V ++GGPRVGN RF++R EELGVKV+RVVNV+D + K PG+F            
Sbjct: 345 KVIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHALMKI 404

Query: 388 -------YEHVGVELVLDFFNMQ------NPSCVHDLETYISLLRYPKEEHQEHDDDHHH 447
                  Y HVG EL LD  N        + S  H+LE  + LL            D +H
Sbjct: 405 AEGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLL------------DGYH 464

BLAST of Csor.00g186100 vs. TAIR 10
Match: AT2G30550.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 285.8 bits (730), Expect = 6.0e-77
Identity = 160/344 (46.51%), Postives = 212/344 (61.63%), Query Frame = 0

Query: 88  WRQIHGCNDWEHLVEPTLHPLLRREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLL 147
           WR+I G +DW  L++P + P+LR E+IRYGE   ACY AFD DP SK   T +F +    
Sbjct: 105 WRKIQGEDDWAGLMDP-MDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFF 164

Query: 148 KEVGLESSGYEVTKYIYATPPDINIPPIQNSP------PSCGRWIGYVAVSSDETS-ERL 207
             +G+  SGYEV +Y+YAT  +IN+P   +             W+GYVAVS DETS  RL
Sbjct: 165 DSLGMIDSGYEVARYLYAT-SNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSRNRL 224

Query: 208 GRRDVVITFRGTVTNSEWIANLMSSLTPARLDP-HNHRPDVKVESGFLTLYTCEETSTKF 267
           GRRD+ I +RGTVT  EWIA+L   L P   +      P VKVESGFL LYT ++T+ KF
Sbjct: 225 GRRDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPDPAVKVESGFLDLYTDKDTTCKF 284

Query: 268 GLESCREQLLSEISRLLNKYKSE---EVSITMAGHSMGSALALLLAYDIAELGLNKRTNK 327
              S REQ+L+E+ RL+ ++  +   ++SIT+ GHS+G ALA+L AYDIAE+ LN+    
Sbjct: 285 ARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSKKG 344

Query: 328 EVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDPITKMPGVFGG---------- 387
           +V+PV+V ++GGPRVGN RF++R EELGVKV+RVVNV+D + K PG+F            
Sbjct: 345 KVIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHALMKI 404

Query: 388 -------YEHVGVELVLDFFNMQ------NPSCVHDLETYISLL 398
                  Y HVG EL LD  N        + S  H+LE  + LL
Sbjct: 405 AEGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLL 446

BLAST of Csor.00g186100 vs. TAIR 10
Match: AT1G06800.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 281.6 bits (719), Expect = 1.1e-75
Identity = 187/473 (39.53%), Postives = 262/473 (55.39%), Query Frame = 0

Query: 17  FSETTRPVSHSFGQVSLPRKSDHVSSTSRRSVSSSSSSSVLVPLIDMSSSSSSSTSAAVA 76
           +S + RP       ++ P +S    + SR    +S S+ +      ++++         A
Sbjct: 20  YSLSFRPHFSRSTLITFPARSSPARAMSRTDEEASISTRLEQESYGLTTAEDIRRRDGEA 79

Query: 77  MVHKVAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIRYGEFVTACYKAFDLDPNSKRY 136
              K   L   WR+I G +DW  L++P + P+LR E+IRYGE   ACY AFD DP S+  
Sbjct: 80  KESK--RLRDTWRKIQGEDDWAGLMDP-MDPVLRSELIRYGEMAQACYDAFDFDPFSRYC 139

Query: 137 LTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNSP------PSCGRWIGYVA 196
            +C+F ++ L   +G+  SGYEV +Y+YAT  +IN+P   +             W+GYVA
Sbjct: 140 GSCRFTRRHLFDSLGIIDSGYEVARYLYAT-SNINLPNFFSKSRWSKVWSKNANWMGYVA 199

Query: 197 VSSDE--TSERLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHN-HRPDVKVESGFL 256
           VS D   T  RLGRRD+ I +RGTVT  EWIA+L   L P   +      P VK ESGFL
Sbjct: 200 VSDDNEATRCRLGRRDIAIAWRGTVTRLEWIADLKDFLKPVSGNGFRCPDPAVKAESGFL 259

Query: 257 TLYTCEETSTKFGLESCREQLLSEISRLLNKY---KSEEVSITMAGHSMGSALALLLAYD 316
            LYT ++TS  F   S REQ+L+E+ RL+ +Y   + EE+SIT+ GHS+G ALA+L AYD
Sbjct: 260 DLYTDKDTSCNFSKFSAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGGALAVLSAYD 319

Query: 317 IAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDPITKMPGVF 376
           +AE+G+N+    +V+PV+ F++GGPRVGN RFK+R E+LGVKVLRVVN +D + K PG+F
Sbjct: 320 VAEMGVNRTRKGKVIPVTAFTYGGPRVGNIRFKERIEKLGVKVLRVVNEHDVVAKSPGLF 379

Query: 377 -------------GG----YEHVGVELVLDFFNMQNP--------SCVHDLETYISLLRY 436
                        GG    Y HVG  L LD  + ++P        S  H+LE  + LL  
Sbjct: 380 LNERAPQALMKLAGGLPWCYSHVGEMLPLD--HQKSPFLKPTVDLSTAHNLEALLHLL-- 439

Query: 437 PKEEHQEHDDDHHHHNGRRKAINGG---DFINKAMEFLSFN-MFP--WRNPMN 447
                     D +H  G+R  ++ G     +NKA +FL  + M P  WR   N
Sbjct: 440 ----------DGYHGKGQRFVLSSGRDPALVNKASDFLKDHFMVPPYWRQDAN 474

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SIN92.7e-13054.53Phospholipase A1-Ialpha2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL5... [more]
Q9MA461.0e-12959.67Galactolipase DONGLE, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DGL PE=1 ... [more]
A2WY001.3e-11361.75Phospholipase A1 EG1, chloroplastic/mitochondrial OS=Oryza sativa subsp. indica ... [more]
Q8S1D91.3e-11361.75Phospholipase A1 EG1, chloroplastic/mitochondrial OS=Oryza sativa subsp. japonic... [more]
Q3EBR61.6e-7743.61Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL3... [more]
Match NameE-valueIdentityDescription
KAG6582093.10.0100.00Galactolipase DONGLE, chloroplastic, partial [Cucurbita argyrosperma subsp. soro... [more]
XP_022956251.10.098.91phospholipase A1-Ialpha2, chloroplastic-like [Cucurbita moschata][more]
XP_022979567.10.097.79phospholipase A1-Ialpha2, chloroplastic-like [Cucurbita maxima][more]
XP_023526912.15.17e-29198.78galactolipase DONGLE, chloroplastic-like, partial [Cucurbita pepo subsp. pepo][more]
XP_004148168.27.73e-27082.14galactolipase DONGLE, chloroplastic [Cucumis sativus] >KGN57254.1 hypothetical p... [more]
Match NameE-valueIdentityDescription
A0A6J1GW190.098.91phospholipase A1-Ialpha2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1IP190.097.79phospholipase A1-Ialpha2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A0A0L8A73.74e-27082.14Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G174570 PE... [more]
A0A5A7VKE22.70e-26980.89Galactolipase DONGLE OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577... [more]
A0A1S3AXV12.70e-26980.89galactolipase DONGLE, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103483973... [more]
Match NameE-valueIdentityDescription
AT2G31690.11.9e-13154.53alpha/beta-Hydrolases superfamily protein [more]
AT1G05800.17.3e-13159.67alpha/beta-Hydrolases superfamily protein [more]
AT2G30550.21.1e-7843.61alpha/beta-Hydrolases superfamily protein [more]
AT2G30550.16.0e-7746.51alpha/beta-Hydrolases superfamily protein [more]
AT1G06800.11.1e-7539.53alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 265..285
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 28..50
NoneNo IPR availablePANTHERPTHR31403:SF4PHOSPHOLIPASE A1-IALPHA2, CHLOROPLASTICcoord: 22..436
NoneNo IPR availablePANTHERPTHR31403PHOSPHOLIPASE A1-IBETA2, CHLOROPLASTICcoord: 22..436
NoneNo IPR availableCDDcd00519Lipase_3coord: 150..398
e-value: 1.19486E-50
score: 169.579
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 206..363
e-value: 5.1E-39
score: 133.4
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 87..438
e-value: 3.2E-100
score: 338.1
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 168..391

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g186100.m01Csor.00g186100.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009695 jasmonic acid biosynthetic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0008970 phospholipase A1 activity