Csor.00g184200 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g184200
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionNEDD8-activating enzyme E1 catalytic subunit
LocationCsor_Chr14: 14765746 .. 14770143 (+)
RNA-Seq ExpressionCsor.00g184200
SyntenyCsor.00g184200
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGATTCTGTTGTCCAATCGAGTCGATCGAGGGACCTCGATAAGCTCCTTCTTAGGCCCGGTAACCTCATCGGCGCCAATTTCGAGCCCGGTTCCCAAGTGGGTACTTCTTCTCTCCGACATTTTATCAAATACCTTGTGCTCAATACATGGTCTGCAATCGTCTTACGACTAAGTTGATTTGTATTACAGTTGAGGGATGATCTTCAACAGTATGTGAAAGTTCTGGTCATAGGAGCTGGAGGGTTGGGCTGCGAGCTCCTGAAGGACTTGGCTTTGTCGGGTTTCCGGAATCTGGAAGTAATTGACATGGATCGCATTGAAGTTACTAATCTCAATCGTCAGTTTCTCTTCAGGTTCCAACTAATTTCTTGGTTCTTTATGAATCTTTGGAGTTCTATTCGTAGTGTTCTTTTGAAATTTTGAACAGCCGGGGCCTGTGGGGTGGGTATAGAATAGAACCTTTACATTTTGTAGGGTGAATACTGTGCTGTAATGAATTGAAAATTGACCTCTATTTTCAATTTACATCCATTATTGACTGATGATATCTATATTTATGTTTGTTAAGTGGAAAGGTTTCACCTGTTCTAGGAATTAGATGGGTGGAATGTTCTTAACCAAAGCATGGTGCTTAAATAGATTTTCCTCAGAGAAAGCTCTTTTCAGGACATTTTCTTGATACGATATGATTTGATCTTGGGCTCATTGAGGAAATCTCTTTCTCATATGTGCATTTGCATATGGCTTTACAATAGTCATTTGATTAACTATGGCTGTCAGGAGAATGACTCTATGTAACATTGTAGGCTTGATGATGTTGGTAAGCCAAAAGCTGAGGTAGCTGCAAAACGTGTTATGGAGAGAGTTAGTGGTGTCAATATTGTGTGGCATTTTTGTCGGATTGAGGACAAAGAAATTGAATTTTACAACGATTTTCACATAATTGCACTTGGTCTTGATTCCATTGAAGCTCGAAGTTACATCAATTCTGTCGCCTGTAGCTTCCTTGGTATGCTATGCTCGTACTTGCTACATTTTACTAATGTCTCTTCACCATCTTGTGTCCGGTAAATTTTAATGTCATGTCAATGATGAATGCAAACTTCATGTCCTTTATTTTCTCTTTGTAACCTCTAGAGTACGATTCTGATGATAATCCGTTAGAAGAAACAATTAAACCAATGGTAGATGGTGGAACTGAAGGCTTCAAGGGGCATGCAAGGGTAATTTTACCAGGGGTCACGCCATGCTTCGAGTGTACCATCTGGCTTTTTCCACCTCAAGTGAAGTTCCCTTTATGTACTCTAGCAGAAACTCCTAGAACTGCCGCTCATTGTATCGAGTATGCTCACCTAATTAAATGGGATGAGGTAATGCATTAGTTACCATGCTTTTCATTGATGATTGTGAATGTATCCCTTCGAGTTACCCAATACTTTTTCTTTTAGGTTCATAGCGGCAAAGCTTTTGATCCGGATGATTCAGAACATATGAAATGGGTTTACAGTGAGGTACATAAATTCCTGTAAGATCTGAGAATTCTTTTCAATTCGTGATTTTACACGTCTAATTTATCTTCCCACATCTATAGGCTGTTAAGAGAGCCGAGCTTTTTGGCATACCCGGAGTTACATATTCACTAACTCAGGTACATATTAGTCTGGGAATTTTCACATTAGTAAACTGATACGTCGATTATATTTTATCAGAACTCGTGATTGTTCTTTTAGTAAGTACATATTATCTGCTTGCATTTTTTTTTAAACTACTCTCTTTGTTGTTACGAATGCACCTACTGATTTACATTTTCTAAGCGATTTTTAGGGTGTTGTGAAGAATATCATCCCTGCTATTGCTTCCACCAATGCATTATATCAGCTGCATGTACACTGGAAACGTTGAAGATCGTATCGGGATGCAGCAAAACGCTATCCAACTACTTAACGTGTGTACTGTTTTCTTCAAACATCTAACAATTCCTTTTCATTTTTTTGTAATATGGGTTTAATAATAATTTTGTGGTGCAACTTAATAATGTTTTTCTTTATTCGTACTCGTAATTATATAGGTAGATAGATAAGATGTCAAGCTGCAATGGGACATTTTGATTTATTCTGTGCAGCATTCTTCGAGTACTGAAGTCATAATCCAGCAGTGTGGTTTATGTTATGATTGCTATCTGTTCACCTTTTCATTTGTTTTTGGTTCTTGTTATGATCTGGAAGTTATTTGGTTCTCATTCTTCCTTCTTGAATTGATAAGTCATTCAATGCCTTGTTCATATACTAAACAATTTTGGTTTCTTTCCATTATTTACTTTTTACTTCTTTTAGATTCGAATTCTAGCCTTTTATAGCCATCCAATCTGTTCTTTCTATCTTTTGGAAGTTGAAATTAGGATTGCGAGTGAATTACACAATTTTCAATGCATTACTTGCTTTATCATATATCCTGGCTGAATTTCTAATACCCAGTAACATTTTTCCATTATTCCTTATATTTGTGGCCTTACTTTCCGAGTGCATTGAATTTAATGCCAAGTGATCCTTTTCCAGATATAATGGTGCAGAAGGTCTCCACACTAAAGTGACAGAGTTTGTAAAAGACAAGGACTGTCTTGTTTGTGGTCCAGGTGTTCTTATTGAGCTGGATTCTTCAATCTCTTTGCAAAAGGTCGGTTATTTAGCTTATGCAAACATATCGTTAATTTCAGATGTTGAGAAATCTTGTAAAATCCTTCATGACGTTACTCCCACTTTTTGTGTGAAACAGTTCATTGAACTTCTAGAGGATCATCCCAAGCTCTTGTTATCGAAGGCTAGTGTCACGCATCGAGGGAAGAATCTATACATGCAGGCACCTCCTGTACTTGAAGAGATGACCAGATCAAATTTGAGTGTTCCACTTTTTGACTTGATGGGTAAAGTTTTGAAAGATGTAGTCCATGTCACTGGTGTAGCTTCTAAGAATGATAAGAAAACTTCCTGTCTCCGAAAATTACGTGTGGCGTTTAAGGGGGTTGACGGTGTTACGGATATGGACACTGCAGGTGGGGCTTAATACTACATTACATTTTTATCTCCAAATTTGAGTCATATGGCTCTCTCTTCCCCCTCTAATGAATGGAAAAAGGTTGATGTGGGGTCGGATCCATTCCCATTTGTACAATTTTTTTTCCTTCCTGAAAAGAACATTAGAGCTGTGAATCTGTTGCCAGAGACTACTGATGTATTGCACAATGATTTACTTCTTTCATCCTTCTACTTCAAGCTACTATGTGACTATTAACTGCTAACAGTGATTAAGACTTGACATAGTGGATATTTTTTTGAAGATTTCCTTGTCATTTGTAATTAGTATCAGCTTTAGCTTGGAAAAATGGGTAGCATGGTTGTTGCTGCTGCTTGCTGCTTGTATTTGAATAATGATACCAATCCTCATGCTTTATGTAAATGTTTATAATGAATTCTTTCACCAGCTGCTGATCCCATATCTGATTGATTTGCTTGCTGAAGACAGGTAATGTTCCTGAAAACCAAGAGATTTACTAATGCTGAATGTTTAATGATATAGTAGAAACAAAGCACCCTTTGACAGGTAATGGTCTAGCAATTACCCCGAGAGGAGGCCTCCAATCACAAAGAAAGTTGGGAAGCAAACATGGAGGGGCCCTCTACCAGCTAGCAAAACGAGTGAATATGTAGAATCCTACTGCCATGATTAATACTTTAGAGCTTTGCTCATTGGGGTTCACTCTAAAGTGGATGAACATAGTGATATGGATATCGATGAGCCTCTGCACTATGTCATGTTCATTCGATTATCTCTAAAGCTGACTGTAGAGGAGAACTGATTCCCCTTCTCTTAAGCTTGACCATAAAAAGATTCAAGTTTATGTTTGATTGTATAGTGACTTTGTTTGCAGGCTTGCGTAGGCTTTACCAAAATCTAAATACAGATCACAGTCTTTGTTTTATTGATCATGAGCCAAACATCCCTTAATTGTTTTTTTATGTTCCCTGACACCACAAATGCAAGTGGCCCCTCGAAAGAGATATCCATCCACCGACTCCTAGAACTAATGGTCTCAAACAACACACAGATGCACGGATGAGTACCTATGACAGTTAGGCTGCTATCTAGTTCAAGTTGAGAGCCAGATAGATCTTCTCCCACAATATCGATCATTTATTGCCTTTTTGAATACATGAGATCAGATCTAAGTCGTATTATGAAACAGACTAATGGGATTTGCTTTGATGAACAGGAAATCCATTAAGAGATGCTCTGCTACAGAAACCCAAGTGCGTAGAAGATTTAAGGCAATCTCATGGCTTGGAGATGGCGATTGGTTGCCGGCAACTGATCCTCATCTTTTCCCCAACTGGATAA

mRNA sequence

ATGGCGGATTCTGTTGTCCAATCGAGTCGATCGAGGGACCTCGATAAGCTCCTTCTTAGGCCCGGTAACCTCATCGGCGCCAATTTCGAGCCCGGTTCCCAATTGAGGGATGATCTTCAACAGTATGTGAAAGTTCTGGTCATAGGAGCTGGAGGGTTGGGCTGCGAGCTCCTGAAGGACTTGGCTTTGTCGGGTTTCCGGAATCTGGAAGTAATTGACATGGATCGCATTGAAGTTACTAATCTCAATCGTCAGTTTCTCTTCAGGCTTGATGATGTTGGTAAGCCAAAAGCTGAGGTAGCTGCAAAACGTGTTATGGAGAGAGTTAGTGGTGTCAATATTGTGTGGCATTTTTGTCGGATTGAGGACAAAGAAATTGAATTTTACAACGATTTTCACATAATTGCACTTGGTCTTGATTCCATTGAAGCTCGAAGTTACATCAATTCTGTCGCCTGTAGCTTCCTTGAGTACGATTCTGATGATAATCCGTTAGAAGAAACAATTAAACCAATGGTAGATGGTGGAACTGAAGGCTTCAAGGGGCATGCAAGGGTAATTTTACCAGGGGTCACGCCATGCTTCGAGTGTACCATCTGGCTTTTTCCACCTCAAGTGAAGTTCCCTTTATGTACTCTAGCAGAAACTCCTAGAACTGCCGCTCATTGTATCGAGTATGCTCACCTAATTAAATGGGATGAGGTTCATAGCGGCAAAGCTTTTGATCCGGATGATTCAGAACATATGAAATGGGTTTACAGTGAGGCTGTTAAGAGAGCCGAGCTTTTTGGCATACCCGGAGTTACATATTCACTAACTCAGGGTGTTATCGTATCGGGATGCAGCAAAACGCTATCCAACTACTTAACATATAATGGTGCAGAAGGTCTCCACACTAAAGTGACAGAGTTTGTAAAAGACAAGGACTGTCTTGTTTGTGGTCCAGGTGTTCTTATTGAGCTGGATTCTTCAATCTCTTTGCAAAAGTTCATTGAACTTCTAGAGGATCATCCCAAGCTCTTGTTATCGAAGGCTAGTGTCACGCATCGAGGGAAGAATCTATACATGCAGGCACCTCCTGTACTTGAAGAGATGACCAGATCAAATTTGAGTGTTCCACTTTTTGACTTGATGGGTAAAGTTTTGAAAGATGTAGTCCATGTCACTGGTGTAGCTTCTAAGAATGATAAGAAAACTTCCTGTCTCCGAAAATTACGTGTGGCGTTTAAGGGGGTTGACGGTGTTACGGATATGGACACTGCAGGAAATCCATTAAGAGATGCTCTGCTACAGAAACCCAAGTGCGTAGAAGATTTAAGGCAATCTCATGGCTTGGAGATGGCGATTGGTTGCCGGCAACTGATCCTCATCTTTTCCCCAACTGGATAA

Coding sequence (CDS)

ATGGCGGATTCTGTTGTCCAATCGAGTCGATCGAGGGACCTCGATAAGCTCCTTCTTAGGCCCGGTAACCTCATCGGCGCCAATTTCGAGCCCGGTTCCCAATTGAGGGATGATCTTCAACAGTATGTGAAAGTTCTGGTCATAGGAGCTGGAGGGTTGGGCTGCGAGCTCCTGAAGGACTTGGCTTTGTCGGGTTTCCGGAATCTGGAAGTAATTGACATGGATCGCATTGAAGTTACTAATCTCAATCGTCAGTTTCTCTTCAGGCTTGATGATGTTGGTAAGCCAAAAGCTGAGGTAGCTGCAAAACGTGTTATGGAGAGAGTTAGTGGTGTCAATATTGTGTGGCATTTTTGTCGGATTGAGGACAAAGAAATTGAATTTTACAACGATTTTCACATAATTGCACTTGGTCTTGATTCCATTGAAGCTCGAAGTTACATCAATTCTGTCGCCTGTAGCTTCCTTGAGTACGATTCTGATGATAATCCGTTAGAAGAAACAATTAAACCAATGGTAGATGGTGGAACTGAAGGCTTCAAGGGGCATGCAAGGGTAATTTTACCAGGGGTCACGCCATGCTTCGAGTGTACCATCTGGCTTTTTCCACCTCAAGTGAAGTTCCCTTTATGTACTCTAGCAGAAACTCCTAGAACTGCCGCTCATTGTATCGAGTATGCTCACCTAATTAAATGGGATGAGGTTCATAGCGGCAAAGCTTTTGATCCGGATGATTCAGAACATATGAAATGGGTTTACAGTGAGGCTGTTAAGAGAGCCGAGCTTTTTGGCATACCCGGAGTTACATATTCACTAACTCAGGGTGTTATCGTATCGGGATGCAGCAAAACGCTATCCAACTACTTAACATATAATGGTGCAGAAGGTCTCCACACTAAAGTGACAGAGTTTGTAAAAGACAAGGACTGTCTTGTTTGTGGTCCAGGTGTTCTTATTGAGCTGGATTCTTCAATCTCTTTGCAAAAGTTCATTGAACTTCTAGAGGATCATCCCAAGCTCTTGTTATCGAAGGCTAGTGTCACGCATCGAGGGAAGAATCTATACATGCAGGCACCTCCTGTACTTGAAGAGATGACCAGATCAAATTTGAGTGTTCCACTTTTTGACTTGATGGGTAAAGTTTTGAAAGATGTAGTCCATGTCACTGGTGTAGCTTCTAAGAATGATAAGAAAACTTCCTGTCTCCGAAAATTACGTGTGGCGTTTAAGGGGGTTGACGGTGTTACGGATATGGACACTGCAGGAAATCCATTAAGAGATGCTCTGCTACAGAAACCCAAGTGCGTAGAAGATTTAAGGCAATCTCATGGCTTGGAGATGGCGATTGGTTGCCGGCAACTGATCCTCATCTTTTCCCCAACTGGATAA

Protein sequence

MADSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGVTDMDTAGNPLRDALLQKPKCVEDLRQSHGLEMAIGCRQLILIFSPTG
Homology
BLAST of Csor.00g184200 vs. ExPASy Swiss-Prot
Match: O65041 (NEDD8-activating enzyme E1 catalytic subunit OS=Arabidopsis thaliana OX=3702 GN=ECR1 PE=1 SV=2)

HSP 1 Score: 668.3 bits (1723), Expect = 6.2e-191
Identity = 329/442 (74.43%), Postives = 362/442 (81.90%), Query Frame = 0

Query: 6   VQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSG 65
           V  S++RDLDKLLLR GNL+   F PG  LRDD++ YV++LVIGAGGLGCELLKDLALSG
Sbjct: 10  VPQSKTRDLDKLLLRHGNLVDPGFVPGPGLRDDIRDYVRILVIGAGGLGCELLKDLALSG 69

Query: 66  FRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKE 125
           FRNLEVIDMDRIEVTNLNRQFLFR++DVGKPKAEVAAKRVMERVSGV IV HF RIEDKE
Sbjct: 70  FRNLEVIDMDRIEVTNLNRQFLFRIEDVGKPKAEVAAKRVMERVSGVEIVPHFSRIEDKE 129

Query: 126 IEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHAR 185
           IEFYNDF+IIALGLDSIEAR YIN VAC FLEY+ DD P  ETIKPMVDGGTEGFKGHAR
Sbjct: 130 IEFYNDFNIIALGLDSIEARKYINGVACGFLEYNEDDTPKRETIKPMVDGGTEGFKGHAR 189

Query: 186 VILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDD 245
           VILPGVTPCFECTI+LFPPQVKFPLCTLAETPR AAHCIEYAHLI+W+ VH GK FDPD+
Sbjct: 190 VILPGVTPCFECTIYLFPPQVKFPLCTLAETPRNAAHCIEYAHLIQWETVHRGKTFDPDE 249

Query: 246 SEHMKWVYSEAVKRAELFGIPGVTYSLTQGV-------------------------IVSG 305
            EHMKWVY EA++RAELFGIPGVTYSLTQGV                         IVS 
Sbjct: 250 PEHMKWVYDEAIRRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSA 309

Query: 306 CSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKL 365
           CSKTL NYLTYNG EGL+T+VT+F +D +CLVCGPG+LIELD+S++L KFIE+LEDHPKL
Sbjct: 310 CSKTLVNYLTYNGGEGLYTEVTKFERDTECLVCGPGILIELDTSVTLSKFIEMLEDHPKL 369

Query: 366 LLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGV-ASKNDKKT 422
           LLSKASV      LYMQAPPVLEE  R  LS PL+DLMG+V KD +HV G  A KN++K 
Sbjct: 370 LLSKASVKQGENTLYMQAPPVLEEFHRPKLSKPLYDLMGRVQKDTIHVFGQRALKNNEKE 429

BLAST of Csor.00g184200 vs. ExPASy Swiss-Prot
Match: Q54QG9 (NEDD8-activating enzyme E1 catalytic subunit OS=Dictyostelium discoideum OX=44689 GN=uba3 PE=1 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 9.5e-91
Identity = 183/412 (44.42%), Postives = 247/412 (59.95%), Query Frame = 0

Query: 1   MADSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQ----LRDDLQQYVKVLVIGAGGLGCE 60
           M  SV    R  D++K++ R G     +FEP ++    + + LQ   KVLVIGAGGLGCE
Sbjct: 4   MDTSVDLPGRWIDIEKIIKRTGPFASPSFEPDTKASPNIMNGLQNDFKVLVIGAGGLGCE 63

Query: 61  LLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVW 120
           +LK+LALSGFRN++VIDMD I+++NLNRQFLFR  DVGK KAEVAA  +  R++G N+  
Sbjct: 64  ILKNLALSGFRNIDVIDMDTIDISNLNRQFLFRRKDVGKSKAEVAAAFINSRITGCNVTP 123

Query: 121 HFCRIEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGG 180
           H CRI+DK+ ++Y  F I+  GLDSIEAR +IN +  + +  +   +   +TI P+VDGG
Sbjct: 124 HKCRIQDKDEDYYRQFKIVIAGLDSIEARRWINGLLVNLVVVNDSGDIEPDTIIPLVDGG 183

Query: 181 TEGFKGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVH 240
           TEGFKG ARVILP ++ CFEC++  FPPQV + +CT+A TPR   HCI++A L    +  
Sbjct: 184 TEGFKGQARVILPKISSCFECSLDAFPPQVSYAICTIANTPRVPEHCIQWALLFGLQDAT 243

Query: 241 SGKAFDP-----DDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGV--------------I 300
             K FDP     D+ +HM W++  A KRAE F I GVTY LTQGV              I
Sbjct: 244 LEKPFDPKQFDNDNPDHMNWLFECAKKRAEKFNINGVTYKLTQGVAKNIIPAIASTNAII 303

Query: 301 VSGC-----------SKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPG-VLIELDSSI 360
            + C           S  L+NY+ YNG  G++T   E+   + C VCG   V  E+D S 
Sbjct: 304 AAACCNEVFKFCTDSSGYLNNYMMYNGLNGVYTFTFEYEIKEGCAVCGTNLVTFEIDKSN 363

Query: 361 SLQKFIELLEDHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDL 378
           +L  F+E +    +    K S+   G+NLYMQ   +L + T  NL   L +L
Sbjct: 364 TLSTFLEKITTDSRFQFKKPSLRSNGRNLYMQG--LLHQSTVPNLEKTLSEL 413

BLAST of Csor.00g184200 vs. ExPASy Swiss-Prot
Match: Q9V6U8 (Nedd8-activating enzyme E1 catalytic subunit OS=Drosophila melanogaster OX=7227 GN=Uba3 PE=1 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 2.4e-89
Identity = 178/407 (43.73%), Postives = 240/407 (58.97%), Query Frame = 0

Query: 5   VVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALS 64
           ++QS R   L  +L R G      F   S+  + LQ   +VL+IGAGGLGCELLKDLAL 
Sbjct: 11  ILQSKRFNGLRNILEREGPFCKDGFAASSENLEFLQTKCQVLIIGAGGLGCELLKDLALM 70

Query: 65  GFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDK 124
           GF NL VIDMD IE++NLNRQFLFR  D+G  KAE AA+ +  RV    +  HF +I+D 
Sbjct: 71  GFGNLHVIDMDTIELSNLNRQFLFRRTDIGASKAECAARFINARVPTCRVTPHFKKIQDF 130

Query: 125 EIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHA 184
           +  FY  FH++  GLDSI AR +IN +  S L Y+ D      +I PM+DGGTEGFKG+A
Sbjct: 131 DESFYQQFHLVVCGLDSIVARRWINGMLLSMLRYEEDGTIDTSSIVPMIDGGTEGFKGNA 190

Query: 185 RVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHS-GKAFDP 244
           RVILPG T C ECT+ LFPPQV +PLCT+A TPR   HCIEY  +I+W++ +  G   D 
Sbjct: 191 RVILPGFTACIECTLDLFPPQVNYPLCTIANTPRLPEHCIEYVKIIQWEKQNPFGVPLDG 250

Query: 245 DDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVI-------------------------V 304
           DD +H+ W+Y  A++R+  F I GVTY L QGV+                          
Sbjct: 251 DDPQHIGWIYERALERSNEFNITGVTYRLVQGVVKHIIPAVASTNAAIAAACALEVFKLA 310

Query: 305 SGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCG--PGVL-IELDSSISLQKFIELLE 364
           + C  +++NYL +N  +G++T   E  K ++CL C   P  L IE  ++ +L+  I+LL 
Sbjct: 311 TSCYDSMANYLNFNDLDGIYTYTYEAEKSENCLACSNTPQPLPIEDPNTTTLEDVIKLLC 370

Query: 365 DHPKLLLSKASVT-----HRGKNLYMQAPPVLEEMTRSNLSVPLFDL 378
           D P+  L   ++T      + + LYM     +EE TR NL+  L +L
Sbjct: 371 DSPRFQLKSPALTTVMKDGKRRTLYMSGVKSIEEATRKNLTQSLGEL 417

BLAST of Csor.00g184200 vs. ExPASy Swiss-Prot
Match: Q99MI7 (NEDD8-activating enzyme E1 catalytic subunit OS=Rattus norvegicus OX=10116 GN=Uba3 PE=1 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 2.6e-88
Identity = 182/402 (45.27%), Postives = 243/402 (60.45%), Query Frame = 0

Query: 10  RSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNL 69
           R   + K L R G     +FEP ++    L    KVLVIGAGGLGCELLK+LALSGFR +
Sbjct: 37  RWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQI 96

Query: 70  EVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFY 129
            VIDMD I+V+NLNRQFLFR  DVG+PKAEVAA+ + +RV   N+V HF +I+D    FY
Sbjct: 97  HVIDMDTIDVSNLNRQFLFRPKDVGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFY 156

Query: 130 NDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLE-ETIKPMVDGGTEGFKGHARVIL 189
             FHII  GLDSI AR +IN +  S L Y  +D  L+  +I P++DGGTEGFKG+ARVIL
Sbjct: 157 RQFHIIVCGLDSIIARRWINGMLISLLNY--EDGVLDPSSIVPLIDGGTEGFKGNARVIL 216

Query: 190 PGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVH---SGKAFDPDD 249
           PG+T C ECT+ L+PPQV FP+CT+A  PR   HCIEY  +++W +      G   D DD
Sbjct: 217 PGMTACIECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGDGVPLDGDD 276

Query: 250 SEHMKWVYSEAVKRAELFGIPGVTYSLTQGV---IVSGCSKT------------------ 309
            EH++W++ ++V+RA  + I GVTY LTQGV   I+   + T                  
Sbjct: 277 PEHIQWIFQKSVERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATS 336

Query: 310 ----LSNYLTYNGAEGLHTKVTEFVKDKDCLVCGP-GVLIELDSSISLQKFIELLEDHPK 369
               L+NYL +N  +GL+T   E  + ++C  C      I+   S  LQ+ ++ L +   
Sbjct: 337 AYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSAS 396

Query: 370 LLLSKASVTH--RGKN--LYMQAPPVLEEMTRSNLSVPLFDL 378
           L +   ++T    GKN  LY+Q+   +EE TR NLS  L +L
Sbjct: 397 LQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKEL 436

BLAST of Csor.00g184200 vs. ExPASy Swiss-Prot
Match: Q8C878 (NEDD8-activating enzyme E1 catalytic subunit OS=Mus musculus OX=10090 GN=Uba3 PE=1 SV=2)

HSP 1 Score: 327.0 bits (837), Expect = 3.4e-88
Identity = 181/402 (45.02%), Postives = 243/402 (60.45%), Query Frame = 0

Query: 10  RSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNL 69
           R   + K L R G     +FEP ++    L    KVLVIGAGGLGCELLK+LALSGFR +
Sbjct: 37  RWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQI 96

Query: 70  EVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFY 129
            VIDMD I+V+NLNRQFLFR  DVG+PKAEVAA+ + +RV   N+V HF +I+D    FY
Sbjct: 97  HVIDMDTIDVSNLNRQFLFRPKDVGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFY 156

Query: 130 NDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLE-ETIKPMVDGGTEGFKGHARVIL 189
             FHII  GLDSI AR +IN +  S L Y  +D  L+  +I P++DGGTEGFKG+ARVIL
Sbjct: 157 RQFHIIVCGLDSIIARRWINGMLISLLNY--EDGVLDPSSIVPLIDGGTEGFKGNARVIL 216

Query: 190 PGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVH---SGKAFDPDD 249
           PG+T C ECT+ L+PPQV FP+CT+A  PR   HCIEY  +++W +      G   D DD
Sbjct: 217 PGMTACIECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGDGVPLDGDD 276

Query: 250 SEHMKWVYSEAVKRAELFGIPGVTYSLTQGV---IVSGCSKT------------------ 309
            EH++W++ ++++RA  + I GVTY LTQGV   I+   + T                  
Sbjct: 277 PEHIQWIFQKSIERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATS 336

Query: 310 ----LSNYLTYNGAEGLHTKVTEFVKDKDCLVCGP-GVLIELDSSISLQKFIELLEDHPK 369
               L+NYL +N  +GL+T   E  + ++C  C      I+   S  LQ+ ++ L +   
Sbjct: 337 AYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSAS 396

Query: 370 LLLSKASVTH--RGKN--LYMQAPPVLEEMTRSNLSVPLFDL 378
           L +   ++T    GKN  LY+Q+   +EE TR NLS  L +L
Sbjct: 397 LQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKEL 436

BLAST of Csor.00g184200 vs. NCBI nr
Match: KAG6582280.1 (NEDD8-activating enzyme E1 catalytic subunit, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 940 bits (2429), Expect = 0.0
Identity = 462/462 (100.00%), Postives = 462/462 (100.00%), Query Frame = 0

Query: 1   MADSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MADSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MADSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240

Query: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVIVSGCSKTLSNYLTYNGAEGLHTK 300
           FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVIVSGCSKTLSNYLTYNGAEGLHTK
Sbjct: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVIVSGCSKTLSNYLTYNGAEGLHTK 300

Query: 301 VTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKLLLSKASVTHRGKNLYMQAPP 360
           VTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKLLLSKASVTHRGKNLYMQAPP
Sbjct: 301 VTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKLLLSKASVTHRGKNLYMQAPP 360

Query: 361 VLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGVTDMDT 420
           VLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGVTDMDT
Sbjct: 361 VLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGVTDMDT 420

Query: 421 AGNPLRDALLQKPKCVEDLRQSHGLEMAIGCRQLILIFSPTG 462
           AGNPLRDALLQKPKCVEDLRQSHGLEMAIGCRQLILIFSPTG
Sbjct: 421 AGNPLRDALLQKPKCVEDLRQSHGLEMAIGCRQLILIFSPTG 462

BLAST of Csor.00g184200 vs. NCBI nr
Match: XP_023528363.1 (NEDD8-activating enzyme E1 catalytic subunit [Cucurbita pepo subsp. pepo])

HSP 1 Score: 845 bits (2182), Expect = 6.60e-308
Identity = 422/447 (94.41%), Postives = 422/447 (94.41%), Query Frame = 0

Query: 1   MADSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MADSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MADSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240

Query: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGV------------------------ 300
           FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGV                        
Sbjct: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETL 300

Query: 301 -IVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLE 360
            IVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKN 420
           DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKN
Sbjct: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTAG 422
           DKKTSCLRKLRVAFKGVDGVTDMDTAG
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTAG 447

BLAST of Csor.00g184200 vs. NCBI nr
Match: XP_022955969.1 (NEDD8-activating enzyme E1 catalytic subunit [Cucurbita moschata])

HSP 1 Score: 840 bits (2170), Expect = 4.45e-306
Identity = 420/447 (93.96%), Postives = 420/447 (93.96%), Query Frame = 0

Query: 1   MADSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MADSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MADSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240

Query: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGV------------------------ 300
           FDPDDSEHMKWVYSEAV RAELFGIPGVTYSLTQGV                        
Sbjct: 241 FDPDDSEHMKWVYSEAVTRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETL 300

Query: 301 -IVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLE 360
            IVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKN 420
           DHPKLLL KASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKN
Sbjct: 361 DHPKLLLLKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTAG 422
           DKKTSCLRKLRVAFKGVDGVTDMDTAG
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTAG 447

BLAST of Csor.00g184200 vs. NCBI nr
Match: XP_022979511.1 (NEDD8-activating enzyme E1 catalytic subunit [Cucurbita maxima])

HSP 1 Score: 839 bits (2168), Expect = 8.97e-306
Identity = 419/447 (93.74%), Postives = 419/447 (93.74%), Query Frame = 0

Query: 1   MADSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           M DSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MTDSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNP EETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPFEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240

Query: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGV------------------------ 300
           FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGV                        
Sbjct: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETL 300

Query: 301 -IVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLE 360
            IVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKN 420
           DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKV KDVVHVTGVASKN
Sbjct: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVWKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTAG 422
           DKKTSCLRKLRVAFKGVDGVTDMDTAG
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTAG 447

BLAST of Csor.00g184200 vs. NCBI nr
Match: XP_038897781.1 (NEDD8-activating enzyme E1 catalytic subunit [Benincasa hispida])

HSP 1 Score: 827 bits (2135), Expect = 9.57e-301
Identity = 413/446 (92.60%), Postives = 417/446 (93.50%), Query Frame = 0

Query: 1   MADSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MA S+VQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MAGSIVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRL+DVGKPKAEVAAKRVMERVSGVNIV HFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNIVPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240

Query: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGV------------------------ 300
           FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGV                        
Sbjct: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 -IVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLE 360
            IVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSI+LQKFI+LLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKN 420
           DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNL VPLFDLMGKVLKD VHVTGVASKN
Sbjct: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDAVHVTGVASKN 420

BLAST of Csor.00g184200 vs. ExPASy TrEMBL
Match: A0A6J1GXR3 (NEDD8-activating enzyme E1 catalytic subunit OS=Cucurbita moschata OX=3662 GN=LOC111457804 PE=3 SV=1)

HSP 1 Score: 840 bits (2170), Expect = 2.15e-306
Identity = 420/447 (93.96%), Postives = 420/447 (93.96%), Query Frame = 0

Query: 1   MADSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MADSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MADSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240

Query: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGV------------------------ 300
           FDPDDSEHMKWVYSEAV RAELFGIPGVTYSLTQGV                        
Sbjct: 241 FDPDDSEHMKWVYSEAVTRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETL 300

Query: 301 -IVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLE 360
            IVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKN 420
           DHPKLLL KASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKN
Sbjct: 361 DHPKLLLLKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTAG 422
           DKKTSCLRKLRVAFKGVDGVTDMDTAG
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTAG 447

BLAST of Csor.00g184200 vs. ExPASy TrEMBL
Match: A0A6J1IQZ5 (NEDD8-activating enzyme E1 catalytic subunit OS=Cucurbita maxima OX=3661 GN=LOC111479191 PE=3 SV=1)

HSP 1 Score: 839 bits (2168), Expect = 4.34e-306
Identity = 419/447 (93.74%), Postives = 419/447 (93.74%), Query Frame = 0

Query: 1   MADSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           M DSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MTDSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNP EETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPFEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240

Query: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGV------------------------ 300
           FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGV                        
Sbjct: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETL 300

Query: 301 -IVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLE 360
            IVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKN 420
           DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKV KDVVHVTGVASKN
Sbjct: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVWKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTAG 422
           DKKTSCLRKLRVAFKGVDGVTDMDTAG
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTAG 447

BLAST of Csor.00g184200 vs. ExPASy TrEMBL
Match: A0A0A0L8U2 (NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis sativus OX=3659 GN=Csa_3G149920 PE=3 SV=1)

HSP 1 Score: 825 bits (2131), Expect = 1.89e-300
Identity = 412/446 (92.38%), Postives = 418/446 (93.72%), Query Frame = 0

Query: 1   MADSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MA SVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRL+DVGKPKAEVAAKRVMERVSGVNI+ HFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGK+
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKS 240

Query: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGV------------------------ 300
           FDPDDSEHMKWVYSEA+KRAELFGIPGVTYSLTQGV                        
Sbjct: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 -IVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLE 360
            IVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSI+LQKFI+LLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKN 420
           DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNL VPLFDLMGKVLKDVVHVTGVASKN
Sbjct: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420

BLAST of Csor.00g184200 vs. ExPASy TrEMBL
Match: A0A1S3AXQ8 (NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis melo OX=3656 GN=LOC103483734 PE=3 SV=1)

HSP 1 Score: 824 bits (2128), Expect = 5.40e-300
Identity = 412/446 (92.38%), Postives = 418/446 (93.72%), Query Frame = 0

Query: 1   MADSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MA SVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRL+DVGKPKAEVAAKRVMERVSGVNI+ HFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240

Query: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGV------------------------ 300
           FDPDDSEHMKWVYSEA+KRAELFGIPGVTYSLTQGV                        
Sbjct: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 -IVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLE 360
            IVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSI+LQKFI+LLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKN 420
           D+PKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNL VPLFDLMGKVLKDVVHVTGVASKN
Sbjct: 361 DYPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420

BLAST of Csor.00g184200 vs. ExPASy TrEMBL
Match: A0A5D3D1R8 (NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00180 PE=3 SV=1)

HSP 1 Score: 822 bits (2124), Expect = 2.20e-299
Identity = 411/446 (92.15%), Postives = 417/446 (93.50%), Query Frame = 0

Query: 1   MADSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MA SVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRL+DVGKPKAEVAAKRVMERVSGVNI+ HFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240

Query: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGV------------------------ 300
           FDPDDSEHMKWVYSEA+KRAELFGIPGVTYSLTQGV                        
Sbjct: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 -IVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLE 360
            IVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSI+LQKFI+LLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKN 420
           D+PKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNL VPLFDLMGKVLKDVVHVTGVASKN
Sbjct: 361 DYPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420

BLAST of Csor.00g184200 vs. TAIR 10
Match: AT5G19180.1 (E1 C-terminal related 1 )

HSP 1 Score: 668.3 bits (1723), Expect = 4.4e-192
Identity = 329/442 (74.43%), Postives = 362/442 (81.90%), Query Frame = 0

Query: 6   VQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSG 65
           V  S++RDLDKLLLR GNL+   F PG  LRDD++ YV++LVIGAGGLGCELLKDLALSG
Sbjct: 10  VPQSKTRDLDKLLLRHGNLVDPGFVPGPGLRDDIRDYVRILVIGAGGLGCELLKDLALSG 69

Query: 66  FRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKE 125
           FRNLEVIDMDRIEVTNLNRQFLFR++DVGKPKAEVAAKRVMERVSGV IV HF RIEDKE
Sbjct: 70  FRNLEVIDMDRIEVTNLNRQFLFRIEDVGKPKAEVAAKRVMERVSGVEIVPHFSRIEDKE 129

Query: 126 IEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHAR 185
           IEFYNDF+IIALGLDSIEAR YIN VAC FLEY+ DD P  ETIKPMVDGGTEGFKGHAR
Sbjct: 130 IEFYNDFNIIALGLDSIEARKYINGVACGFLEYNEDDTPKRETIKPMVDGGTEGFKGHAR 189

Query: 186 VILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDD 245
           VILPGVTPCFECTI+LFPPQVKFPLCTLAETPR AAHCIEYAHLI+W+ VH GK FDPD+
Sbjct: 190 VILPGVTPCFECTIYLFPPQVKFPLCTLAETPRNAAHCIEYAHLIQWETVHRGKTFDPDE 249

Query: 246 SEHMKWVYSEAVKRAELFGIPGVTYSLTQGV-------------------------IVSG 305
            EHMKWVY EA++RAELFGIPGVTYSLTQGV                         IVS 
Sbjct: 250 PEHMKWVYDEAIRRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSA 309

Query: 306 CSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKL 365
           CSKTL NYLTYNG EGL+T+VT+F +D +CLVCGPG+LIELD+S++L KFIE+LEDHPKL
Sbjct: 310 CSKTLVNYLTYNGGEGLYTEVTKFERDTECLVCGPGILIELDTSVTLSKFIEMLEDHPKL 369

Query: 366 LLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGV-ASKNDKKT 422
           LLSKASV      LYMQAPPVLEE  R  LS PL+DLMG+V KD +HV G  A KN++K 
Sbjct: 370 LLSKASVKQGENTLYMQAPPVLEEFHRPKLSKPLYDLMGRVQKDTIHVFGQRALKNNEKE 429

BLAST of Csor.00g184200 vs. TAIR 10
Match: AT2G21470.1 (SUMO-activating enzyme 2 )

HSP 1 Score: 151.0 bits (380), Expect = 2.4e-36
Identity = 79/203 (38.92%), Postives = 118/203 (58.13%), Query Frame = 0

Query: 44  KVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAK 103
           KVL++GAGG+GCELLK LALSGF ++ +IDMD IEV+NLNRQFLFR   VG+ KA+VA  
Sbjct: 14  KVLMVGAGGIGCELLKTLALSGFEDIHIIDMDTIEVSNLNRQFLFRRSHVGQSKAKVARD 73

Query: 104 RVMERVSGVNIVWHFCRIEDKE--IEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSD 163
            V+     +NI  +   +++ E  ++F+  F ++  GLD+++AR ++N +  +       
Sbjct: 74  AVLRFRPNINIRSYHANVKNPEFDVDFFKQFDVVLNGLDNLDARRHVNRLCLA------- 133

Query: 164 DNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAA 223
                    P+V+ GT GF G   V + G T C+EC     P    +P+CT+  TP    
Sbjct: 134 ------ADVPLVESGTTGFLGQVTVHIKGKTECYECQ--TKPAPKTYPVCTITSTPTKFV 193

Query: 224 HCIEYAHLIKWDEVHSGKAFDPD 245
           HCI +A  + + ++   K  D D
Sbjct: 194 HCIVWAKDLLFAKLFGDKNQDND 201

BLAST of Csor.00g184200 vs. TAIR 10
Match: AT2G21470.2 (SUMO-activating enzyme 2 )

HSP 1 Score: 151.0 bits (380), Expect = 2.4e-36
Identity = 79/203 (38.92%), Postives = 118/203 (58.13%), Query Frame = 0

Query: 44  KVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAK 103
           KVL++GAGG+GCELLK LALSGF ++ +IDMD IEV+NLNRQFLFR   VG+ KA+VA  
Sbjct: 14  KVLMVGAGGIGCELLKTLALSGFEDIHIIDMDTIEVSNLNRQFLFRRSHVGQSKAKVARD 73

Query: 104 RVMERVSGVNIVWHFCRIEDKE--IEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSD 163
            V+     +NI  +   +++ E  ++F+  F ++  GLD+++AR ++N +  +       
Sbjct: 74  AVLRFRPNINIRSYHANVKNPEFDVDFFKQFDVVLNGLDNLDARRHVNRLCLA------- 133

Query: 164 DNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAA 223
                    P+V+ GT GF G   V + G T C+EC     P    +P+CT+  TP    
Sbjct: 134 ------ADVPLVESGTTGFLGQVTVHIKGKTECYECQ--TKPAPKTYPVCTITSTPTKFV 193

Query: 224 HCIEYAHLIKWDEVHSGKAFDPD 245
           HCI +A  + + ++   K  D D
Sbjct: 194 HCIVWAKDLLFAKLFGDKNQDND 201

BLAST of Csor.00g184200 vs. TAIR 10
Match: AT2G21470.3 (SUMO-activating enzyme 2 )

HSP 1 Score: 151.0 bits (380), Expect = 2.4e-36
Identity = 79/203 (38.92%), Postives = 118/203 (58.13%), Query Frame = 0

Query: 44  KVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAK 103
           KVL++GAGG+GCELLK LALSGF ++ +IDMD IEV+NLNRQFLFR   VG+ KA+VA  
Sbjct: 14  KVLMVGAGGIGCELLKTLALSGFEDIHIIDMDTIEVSNLNRQFLFRRSHVGQSKAKVARD 73

Query: 104 RVMERVSGVNIVWHFCRIEDKE--IEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSD 163
            V+     +NI  +   +++ E  ++F+  F ++  GLD+++AR ++N +  +       
Sbjct: 74  AVLRFRPNINIRSYHANVKNPEFDVDFFKQFDVVLNGLDNLDARRHVNRLCLA------- 133

Query: 164 DNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAA 223
                    P+V+ GT GF G   V + G T C+EC     P    +P+CT+  TP    
Sbjct: 134 ------ADVPLVESGTTGFLGQVTVHIKGKTECYECQ--TKPAPKTYPVCTITSTPTKFV 193

Query: 224 HCIEYAHLIKWDEVHSGKAFDPD 245
           HCI +A  + + ++   K  D D
Sbjct: 194 HCIVWAKDLLFAKLFGDKNQDND 201

BLAST of Csor.00g184200 vs. TAIR 10
Match: AT5G06460.1 (ubiquitin activating enzyme 2 )

HSP 1 Score: 100.5 bits (249), Expect = 3.7e-21
Identity = 67/206 (32.52%), Postives = 109/206 (52.91%), Query Frame = 0

Query: 32  GSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFR-----NLEVIDMDRIEVTNLNRQF 91
           GS L+  L+   +V V+GAG LGCE LK+LAL G        L V D D IE +NL+RQF
Sbjct: 480 GSTLQKKLED-ARVFVVGAGALGCEFLKNLALMGVSCGTQGKLTVTDDDVIEKSNLSRQF 539

Query: 92  LFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDK-----EIEFYNDFHIIALGLDS 151
           LFR  ++G+ K+ VAA       S +NI     R+  +     +  F+ +  ++   LD+
Sbjct: 540 LFRDWNIGQAKSTVAATAAAGINSRLNIDALQNRVGPETENVFDDSFWENLTVVVNALDN 599

Query: 152 IEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWL 211
           + AR Y++S  C + +            KP+++ GT G K + ++++P +T  +  +   
Sbjct: 600 VTARLYVDS-RCVYFQ------------KPLLESGTLGAKCNTQMVIPHLTENYGAS--R 659

Query: 212 FPPQVKFPLCTLAETPRTAAHCIEYA 228
            PP+ + P+CT+   P    HC+ +A
Sbjct: 660 DPPEKQAPMCTVHSFPHNIDHCLTWA 669

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O650416.2e-19174.43NEDD8-activating enzyme E1 catalytic subunit OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q54QG99.5e-9144.42NEDD8-activating enzyme E1 catalytic subunit OS=Dictyostelium discoideum OX=4468... [more]
Q9V6U82.4e-8943.73Nedd8-activating enzyme E1 catalytic subunit OS=Drosophila melanogaster OX=7227 ... [more]
Q99MI72.6e-8845.27NEDD8-activating enzyme E1 catalytic subunit OS=Rattus norvegicus OX=10116 GN=Ub... [more]
Q8C8783.4e-8845.02NEDD8-activating enzyme E1 catalytic subunit OS=Mus musculus OX=10090 GN=Uba3 PE... [more]
Match NameE-valueIdentityDescription
KAG6582280.10.0100.00NEDD8-activating enzyme E1 catalytic subunit, partial [Cucurbita argyrosperma su... [more]
XP_023528363.16.60e-30894.41NEDD8-activating enzyme E1 catalytic subunit [Cucurbita pepo subsp. pepo][more]
XP_022955969.14.45e-30693.96NEDD8-activating enzyme E1 catalytic subunit [Cucurbita moschata][more]
XP_022979511.18.97e-30693.74NEDD8-activating enzyme E1 catalytic subunit [Cucurbita maxima][more]
XP_038897781.19.57e-30192.60NEDD8-activating enzyme E1 catalytic subunit [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1GXR32.15e-30693.96NEDD8-activating enzyme E1 catalytic subunit OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1IQZ54.34e-30693.74NEDD8-activating enzyme E1 catalytic subunit OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A0A0L8U21.89e-30092.38NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis sativus OX=3659 GN=Csa_3... [more]
A0A1S3AXQ85.40e-30092.38NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A5D3D1R82.20e-29992.15NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis melo var. makuwa OX=1194... [more]
Match NameE-valueIdentityDescription
AT5G19180.14.4e-19274.43E1 C-terminal related 1 [more]
AT2G21470.12.4e-3638.92SUMO-activating enzyme 2 [more]
AT2G21470.22.4e-3638.92SUMO-activating enzyme 2 [more]
AT2G21470.32.4e-3638.92SUMO-activating enzyme 2 [more]
AT5G06460.13.7e-2132.52ubiquitin activating enzyme 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014929E2 bindingSMARTSM01181E2_bind_2coord: 319..410
e-value: 1.4E-36
score: 137.5
IPR014929E2 bindingPFAMPF08825E2_bindcoord: 320..409
e-value: 3.7E-22
score: 78.2
IPR000594THIF-type NAD/FAD binding foldPFAMPF00899ThiFcoord: 37..261
e-value: 3.4E-58
score: 196.9
NoneNo IPR availableGENE3D3.40.50.720coord: 13..306
e-value: 3.9E-65
score: 221.4
NoneNo IPR availableGENE3D3.10.290.20coord: 317..404
e-value: 7.4E-16
score: 59.8
NoneNo IPR availablePANTHERPTHR10953UBIQUITIN-ACTIVATING ENZYME E1coord: 9..389
NoneNo IPR availableCDDcd01488Uba3_RUBcoord: 44..314
e-value: 3.69193E-175
score: 490.715
IPR030468NEDD8-activating enzyme E1 catalytic subunitPANTHERPTHR10953:SF6NEDD8-ACTIVATING ENZYME E1 CATALYTIC SUBUNITcoord: 9..389
IPR033127Ubiquitin-activating enzyme E1, Cys active sitePROSITEPS00865UBIQUITIN_ACTIVAT_2coord: 209..217
IPR035985Ubiquitin-activating enzymeSUPERFAMILY69572Activating enzymes of the ubiquitin-like proteinscoord: 44..381

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g184200.m01Csor.00g184200.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045116 protein neddylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0019781 NEDD8 activating enzyme activity
molecular_function GO:0008641 ubiquitin-like modifier activating enzyme activity