Csor.00g183290 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g183290
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionenoyl-CoA delta isomerase 2, peroxisomal-like
LocationCsor_Chr14: 15181307 .. 15182026 (+)
RNA-Seq ExpressionCsor.00g183290
SyntenyCsor.00g183290
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSsinglestart_codonpolypeptidestop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGCACAGTTGAGAAGCGAGGCAATTTCTTCATCCTAACACTGACCGGCGACGACGATCACCGCCTCGGCCCATCCTTAATCAACTCCATTCTTAAAGCCCTTTCACAAGTTAAGGCACAAGCCACCTCTGGTTCAGTTCTCATCACAACTTCCCATGGTAAATTCTTCTCAAACGGCTTCGATCTCCCCTGGGCTCTTGCGGCCGGCTCCATTTCTGGTGCCCGTGACCGCCTAAACCACATAATCCAGATCTTCAAGCCCCTCGTAGCCCAACTCCTTTCCCTTCCTATGCCAACCATCGCTGCCATCGCAGGACACGCCGCTGCAGCTGGTTTCGCATTGGCGCTCAGCCACGACTATTTGATTATGAGGAGCGATCGGGGCGTACTATACATGCGTGAGTTGGATCTGGGTCTTACACTGCCGGACTACTTCACGGCACTGTTTAAGTCCAAGATGGGCTCCAGTTTGGCTACGCGCGACCTGATTCTGAGGGGGATGAAAGTGAGAGGTGAAGCGGCGGTCAGAATGGGCGTTGTGGAATCGACGCACCACGGCGAGGACGGCGTCTTCAACGCTGCAGTGCAGCTTGGGGAGCAGCTGGCGGCAAGAAAGTGGAACGGAGAAGTGTATGCTGAGATCAGGAAGAGTTTGTATCCTGAGGTGTCTGAGGTGCTTGGGTTACGGGGGAAGCCCATATCCATTTCTAAACTATGA

mRNA sequence

ATGTGCACAGTTGAGAAGCGAGGCAATTTCTTCATCCTAACACTGACCGGCGACGACGATCACCGCCTCGGCCCATCCTTAATCAACTCCATTCTTAAAGCCCTTTCACAAGTTAAGGCACAAGCCACCTCTGGTTCAGTTCTCATCACAACTTCCCATGGTAAATTCTTCTCAAACGGCTTCGATCTCCCCTGGGCTCTTGCGGCCGGCTCCATTTCTGGTGCCCGTGACCGCCTAAACCACATAATCCAGATCTTCAAGCCCCTCGTAGCCCAACTCCTTTCCCTTCCTATGCCAACCATCGCTGCCATCGCAGGACACGCCGCTGCAGCTGGTTTCGCATTGGCGCTCAGCCACGACTATTTGATTATGAGGAGCGATCGGGGCGTACTATACATGCGTGAGTTGGATCTGGGTCTTACACTGCCGGACTACTTCACGGCACTGTTTAAGTCCAAGATGGGCTCCAGTTTGGCTACGCGCGACCTGATTCTGAGGGGGATGAAAGTGAGAGGTGAAGCGGCGGTCAGAATGGGCGTTGTGGAATCGACGCACCACGGCGAGGACGGCGTCTTCAACGCTGCAGTGCAGCTTGGGGAGCAGCTGGCGGCAAGAAAGTGGAACGGAGAAGTGTATGCTGAGATCAGGAAGAGTTTGTATCCTGAGGTGTCTGAGGTGCTTGGGTTACGGGGGAAGCCCATATCCATTTCTAAACTATGA

Coding sequence (CDS)

ATGTGCACAGTTGAGAAGCGAGGCAATTTCTTCATCCTAACACTGACCGGCGACGACGATCACCGCCTCGGCCCATCCTTAATCAACTCCATTCTTAAAGCCCTTTCACAAGTTAAGGCACAAGCCACCTCTGGTTCAGTTCTCATCACAACTTCCCATGGTAAATTCTTCTCAAACGGCTTCGATCTCCCCTGGGCTCTTGCGGCCGGCTCCATTTCTGGTGCCCGTGACCGCCTAAACCACATAATCCAGATCTTCAAGCCCCTCGTAGCCCAACTCCTTTCCCTTCCTATGCCAACCATCGCTGCCATCGCAGGACACGCCGCTGCAGCTGGTTTCGCATTGGCGCTCAGCCACGACTATTTGATTATGAGGAGCGATCGGGGCGTACTATACATGCGTGAGTTGGATCTGGGTCTTACACTGCCGGACTACTTCACGGCACTGTTTAAGTCCAAGATGGGCTCCAGTTTGGCTACGCGCGACCTGATTCTGAGGGGGATGAAAGTGAGAGGTGAAGCGGCGGTCAGAATGGGCGTTGTGGAATCGACGCACCACGGCGAGGACGGCGTCTTCAACGCTGCAGTGCAGCTTGGGGAGCAGCTGGCGGCAAGAAAGTGGAACGGAGAAGTGTATGCTGAGATCAGGAAGAGTTTGTATCCTGAGGTGTCTGAGGTGCTTGGGTTACGGGGGAAGCCCATATCCATTTCTAAACTATGA

Protein sequence

MCTVEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATSGSVLITTSHGKFFSNGFDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHDYLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGVVESTHHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPISISKL
Homology
BLAST of Csor.00g183290 vs. ExPASy Swiss-Prot
Match: O23299 (Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI2 PE=1 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 6.7e-80
Identity = 146/240 (60.83%), Postives = 191/240 (79.58%), Query Frame = 0

Query: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATSGSVLITTSHGKFFSNG 60
           MCT+EKRG+ F+LTLTGD +HR  P  I +IL  L Q K+Q+T GS+LITT++GKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGSILITTANGKFFSNG 60

Query: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120
           FDL WA  AGS +GA +RL+ +++ FKP+VA LL LPMPTIAA+ GHAAAAG  LALSHD
Sbjct: 61  FDLAWAQTAGSKTGAANRLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGLILALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGV 180
           Y+ MR DRGVLYM E+D+GL++PDYF+AL ++K+G+S A R+L+L G K+RGE AV +G+
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGEEAVGLGI 180

Query: 181 VES-THHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPISISKL 240
           V+S  +  E+GV  A+V+LGE+LAA+KW+GEVYA IRKSLYPE+  +LGL  +  +  KL
Sbjct: 181 VDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGILGLETRVFATPKL 240

BLAST of Csor.00g183290 vs. ExPASy Swiss-Prot
Match: O23300 (Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana OX=3702 GN=ECI3 PE=1 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 1.7e-75
Identity = 140/240 (58.33%), Postives = 180/240 (75.00%), Query Frame = 0

Query: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATSGSVLITTSHGKFFSNG 60
           MCT+EKRG+ F+LTLTG+D+HR  P  I S+L  L Q K+Q+T GSVLITT HGKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGSVLITTGHGKFFSNG 60

Query: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120
           FDL WA +AG   GA  R++ +++ FKP++A LL LPMPTIAA+ GHAAA+G   ALSHD
Sbjct: 61  FDLAWAQSAG--HGAIKRMHQMVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGV 180
           Y+ MR DRGVLYM E+D+GL +PDYF+AL  +K+GS +A R+L+L G K++GE AV +G+
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGEEAVALGI 180

Query: 181 VES-THHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPISISKL 240
           V+S  H   +GV  A V LGE LAA+KWNGEVYA IRKSLYPE+  ++ L    ++   L
Sbjct: 181 VDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMVDLTANNLATHNL 238

BLAST of Csor.00g183290 vs. ExPASy Swiss-Prot
Match: O04469 (Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI1 PE=1 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.0e-51
Identity = 108/224 (48.21%), Postives = 152/224 (67.86%), Query Frame = 0

Query: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQAT-SGSVLITTSHGKFFSN 60
           MC++EKR   FIL LTGD +HRL P+L++S+   ++Q+++  + S SVLITTS GKFFSN
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSFSQSVLITTSDGKFFSN 60

Query: 61  GFDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSH 120
           G+DL  A +  S+S   D         + LVA L+SLPMPTIAA+ GHA+AAG  LA+SH
Sbjct: 61  GYDLALAESNPSLSVVMDAK------LRSLVADLISLPMPTIAAVTGHASAAGCILAMSH 120

Query: 121 DYLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMG 180
           DY++MR DRG LYM ELD+ L +P +F A+ + K+GS  A RD++L   KV  +  V+MG
Sbjct: 121 DYVLMRRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTADVGVKMG 180

Query: 181 VVESTHHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEV 224
           +V+S +        AA++LGE++  R  +G VY ++R+SL  EV
Sbjct: 181 IVDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREV 218

BLAST of Csor.00g183290 vs. ExPASy Swiss-Prot
Match: A1JK30 (Fatty acid oxidation complex subunit alpha OS=Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081) OX=393305 GN=fadJ PE=3 SV=2)

HSP 1 Score: 60.1 bits (144), Expect = 4.0e-08
Identity = 55/192 (28.65%), Postives = 91/192 (47.40%), Query Frame = 0

Query: 9   NFFILT--LTGDDDHRLGPSLINSILKALSQVKAQATSGSVLITTSHGKFFSNGFDLPWA 68
           N  I+T  + GD  + L       I + L Q +A +    ++I +     F  G D+   
Sbjct: 34  NIGIITVDVVGDKVNTLKAKFAEQIAEILQQAQALSQLQGLVIISGKPDSFIAGADITMI 93

Query: 69  LAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALS-HDYLIMR 128
            A  +   AR     + Q  + ++AQ+ + P+P +AAI G     G  LAL+ H  +  +
Sbjct: 94  AACHTAQDAR----ILAQKGQSILAQIAAFPVPVVAAIHGACLGGGLELALACHSRICSQ 153

Query: 129 SDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGVVESTH 188
            D+ VL + E+ LGL      T      +G S A  D+IL G +VR   A++MG+V+   
Sbjct: 154 DDKTVLGLPEVQLGLLPGSGGTQRLPRLVGVSKAL-DMILTGRQVRARQALKMGLVDDV- 213

Query: 189 HGEDGVFNAAVQ 198
             +D + + A+Q
Sbjct: 214 VPQDILLDVAIQ 219

BLAST of Csor.00g183290 vs. ExPASy Swiss-Prot
Match: A7FGK1 (Fatty acid oxidation complex subunit alpha OS=Yersinia pseudotuberculosis serotype O:1b (strain IP 31758) OX=349747 GN=fadJ PE=3 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 7.6e-07
Identity = 51/177 (28.81%), Postives = 81/177 (45.76%), Query Frame = 0

Query: 9   NFFILTL--TGDDDHRLGPSLINSILKALSQVKAQATSGSVLITTSHGKFFSNGFDLPWA 68
           N  I+T+   GD  + L     + I   L Q  A      ++I +     F  G D+   
Sbjct: 22  NIGIITIDVVGDKVNTLKAEFADQIATILQQAHALPKLQGLVIVSGKPDSFIAGADITMI 81

Query: 69  LAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALS-HDYLIMR 128
            A  +   AR     + Q  + ++AQ+ + P+P +AAI G     G  LAL+ H  +   
Sbjct: 82  AACRTAHDAR----VLAQKGQSILAQIAAFPVPVVAAIHGACLGGGLELALACHSRICSL 141

Query: 129 SDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGVVE 183
            D+ VL + E+ LGL      T      +G S A  D+IL G ++R   A++MG+V+
Sbjct: 142 DDKTVLGLPEVQLGLLPGSGGTQRLPRLVGVSKAL-DMILTGKQIRPRQALKMGLVD 193

BLAST of Csor.00g183290 vs. NCBI nr
Match: KAG6582371.1 (Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 464 bits (1194), Expect = 1.26e-164
Identity = 239/239 (100.00%), Postives = 239/239 (100.00%), Query Frame = 0

Query: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATSGSVLITTSHGKFFSNG 60
           MCTVEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATSGSVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATSGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120
           FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD
Sbjct: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGV 180
           YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGV
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGV 180

Query: 181 VESTHHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPISISKL 239
           VESTHHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPISISKL
Sbjct: 181 VESTHHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPISISKL 239

BLAST of Csor.00g183290 vs. NCBI nr
Match: XP_022924234.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita moschata])

HSP 1 Score: 458 bits (1178), Expect = 3.48e-162
Identity = 235/239 (98.33%), Postives = 237/239 (99.16%), Query Frame = 0

Query: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATSGSVLITTSHGKFFSNG 60
           MCTVEKRGNFFILTLTGDDDHRLGPSLINS+LKALSQVKAQAT GSVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSLLKALSQVKAQATPGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120
           FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD
Sbjct: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGV 180
           YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGV
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGV 180

Query: 181 VESTHHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPISISKL 239
           VESTHHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPI IS+L
Sbjct: 181 VESTHHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPIFISRL 239

BLAST of Csor.00g183290 vs. NCBI nr
Match: XP_023526416.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 450 bits (1157), Expect = 5.53e-159
Identity = 231/239 (96.65%), Postives = 236/239 (98.74%), Query Frame = 0

Query: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATSGSVLITTSHGKFFSNG 60
           MCTVEKRGNFFILTLTGDDDHRLGPSLINS+LKALSQVKAQAT GSVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSLLKALSQVKAQATPGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120
           FDLPWALAAGSISGARDRL+HIIQIFKPLVAQLLSLPMPTIAAI+GHAAAAGFALALSHD
Sbjct: 61  FDLPWALAAGSISGARDRLSHIIQIFKPLVAQLLSLPMPTIAAISGHAAAAGFALALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGV 180
           YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLA RDLILRGMKVRGEAAVRMGV
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLAMRDLILRGMKVRGEAAVRMGV 180

Query: 181 VESTHHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPISISKL 239
           VESTHHGEDGVFNAAVQLGEQLAARKW+GEVYAEIRKS+YPEVSEVLGLRGK ISISKL
Sbjct: 181 VESTHHGEDGVFNAAVQLGEQLAARKWDGEVYAEIRKSVYPEVSEVLGLRGKAISISKL 239

BLAST of Csor.00g183290 vs. NCBI nr
Match: XP_022979202.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita maxima])

HSP 1 Score: 447 bits (1149), Expect = 9.16e-158
Identity = 231/239 (96.65%), Postives = 234/239 (97.91%), Query Frame = 0

Query: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATSGSVLITTSHGKFFSNG 60
           MCT+EKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQAT GSVLITTSHGKFFSNG
Sbjct: 1   MCTLEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATPGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120
           FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD
Sbjct: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGV 180
           YLIMRSDRGVLYMRELDLGLTLPDYFTALF SKMGSSLA RDLILRGMKVRGEAAVR+GV
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFMSKMGSSLAMRDLILRGMKVRGEAAVRIGV 180

Query: 181 VESTHHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPISISKL 239
           VESTHHGEDGV NAAVQLGEQLAARKW+GEVYAEIRKSLYPEVSEVLGLRGK ISISKL
Sbjct: 181 VESTHHGEDGVANAAVQLGEQLAARKWDGEVYAEIRKSLYPEVSEVLGLRGKAISISKL 239

BLAST of Csor.00g183290 vs. NCBI nr
Match: KAG7018780.1 (Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 430 bits (1106), Expect = 8.15e-145
Identity = 223/228 (97.81%), Postives = 225/228 (98.68%), Query Frame = 0

Query: 5   EKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATSGSVLITTSHGKFFSNGFDLP 64
           EKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATSGSVLITTSHGKFFSNGFDLP
Sbjct: 441 EKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATSGSVLITTSHGKFFSNGFDLP 500

Query: 65  WALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHDYLIM 124
           WALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHDYLIM
Sbjct: 501 WALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHDYLIM 560

Query: 125 RSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGVVEST 184
           RSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGVVEST
Sbjct: 561 RSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGVVEST 620

Query: 185 HHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEV--LGLR 230
           HHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSE   +G+R
Sbjct: 621 HHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEASQMGIR 668

BLAST of Csor.00g183290 vs. ExPASy TrEMBL
Match: A0A6J1E8J9 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=LOC111431761 PE=4 SV=1)

HSP 1 Score: 458 bits (1178), Expect = 1.68e-162
Identity = 235/239 (98.33%), Postives = 237/239 (99.16%), Query Frame = 0

Query: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATSGSVLITTSHGKFFSNG 60
           MCTVEKRGNFFILTLTGDDDHRLGPSLINS+LKALSQVKAQAT GSVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSLLKALSQVKAQATPGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120
           FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD
Sbjct: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGV 180
           YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGV
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGV 180

Query: 181 VESTHHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPISISKL 239
           VESTHHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPI IS+L
Sbjct: 181 VESTHHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPIFISRL 239

BLAST of Csor.00g183290 vs. ExPASy TrEMBL
Match: A0A6J1IN54 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC111479008 PE=4 SV=1)

HSP 1 Score: 447 bits (1149), Expect = 4.44e-158
Identity = 231/239 (96.65%), Postives = 234/239 (97.91%), Query Frame = 0

Query: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATSGSVLITTSHGKFFSNG 60
           MCT+EKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQAT GSVLITTSHGKFFSNG
Sbjct: 1   MCTLEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATPGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120
           FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD
Sbjct: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGV 180
           YLIMRSDRGVLYMRELDLGLTLPDYFTALF SKMGSSLA RDLILRGMKVRGEAAVR+GV
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFMSKMGSSLAMRDLILRGMKVRGEAAVRIGV 180

Query: 181 VESTHHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPISISKL 239
           VESTHHGEDGV NAAVQLGEQLAARKW+GEVYAEIRKSLYPEVSEVLGLRGK ISISKL
Sbjct: 181 VESTHHGEDGVANAAVQLGEQLAARKWDGEVYAEIRKSLYPEVSEVLGLRGKAISISKL 239

BLAST of Csor.00g183290 vs. ExPASy TrEMBL
Match: A0A0A0L9X4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G141770 PE=4 SV=1)

HSP 1 Score: 410 bits (1055), Expect = 9.40e-144
Identity = 209/239 (87.45%), Postives = 222/239 (92.89%), Query Frame = 0

Query: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATSGSVLITTSHGKFFSNG 60
           MCTVEKRGNFFILTLTGDDDHRL PSLINSILKALSQVKAQA+  SVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLSPSLINSILKALSQVKAQASPASVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120
           FDLPWAL+AGS+S AR RLNH+I+IFK +VA+L+SLPMPTIAAI GHAAAAGF LALSHD
Sbjct: 61  FDLPWALSAGSVSAARHRLNHLIEIFKSVVAELISLPMPTIAAITGHAAAAGFTLALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGV 180
           YLIMRSDRGVLYMRELDLGLTLPDYF ALFKSK+GSSLA RDLILRGMKVRGEAAVRMG+
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKIGSSLAMRDLILRGMKVRGEAAVRMGI 180

Query: 181 VESTHHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPISISKL 239
           V+S H GED V NAAV+LGEQLA R W+GEVYAEIRKSLYPEVSEVLGL GKPISISKL
Sbjct: 181 VDSAHDGEDSVVNAAVRLGEQLAGRNWDGEVYAEIRKSLYPEVSEVLGLPGKPISISKL 239

BLAST of Csor.00g183290 vs. ExPASy TrEMBL
Match: A0A1S3AWB3 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC103483618 PE=4 SV=1)

HSP 1 Score: 405 bits (1041), Expect = 1.28e-141
Identity = 207/239 (86.61%), Postives = 219/239 (91.63%), Query Frame = 0

Query: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATSGSVLITTSHGKFFSNG 60
           MCTVEKRGNFFILTLTGDDDHRL PSLINSILKALSQVKAQA+  SVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLSPSLINSILKALSQVKAQASPASVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120
           FDLPWALAAGS+S AR RLNHII+IFK +VA+L+SLPMPTIAAI GHAAAAGF LALSHD
Sbjct: 61  FDLPWALAAGSVSAARHRLNHIIEIFKSVVAELISLPMPTIAAITGHAAAAGFTLALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGV 180
           YLIMRSDRGVLYMRELDLGLTLPDYF ALFKSK+GSSLA RDLILRGMKVRGEAAVRMG+
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKIGSSLAMRDLILRGMKVRGEAAVRMGI 180

Query: 181 VESTHHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPISISKL 239
           V+S HH E  V NAAV+LGEQL  R W+GEVYAEIRKSLYPE+SEVLGL GK ISISKL
Sbjct: 181 VDSAHHSEVSVVNAAVRLGEQLVGRNWDGEVYAEIRKSLYPEISEVLGLPGKAISISKL 239

BLAST of Csor.00g183290 vs. ExPASy TrEMBL
Match: A0A5D3CZF5 (Enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001370 PE=4 SV=1)

HSP 1 Score: 404 bits (1039), Expect = 2.58e-141
Identity = 206/239 (86.19%), Postives = 219/239 (91.63%), Query Frame = 0

Query: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATSGSVLITTSHGKFFSNG 60
           MCTVEKRGNFFILTLTGDDDHRL PSLINSILKALSQVKAQA+  SVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLSPSLINSILKALSQVKAQASPASVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120
           FDLPWALAAGS+S AR R+NHII+IFK +VA+L+SLPMPTIAAI GHAAAAGF LALSHD
Sbjct: 61  FDLPWALAAGSVSAARHRINHIIEIFKSVVAELISLPMPTIAAITGHAAAAGFTLALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGV 180
           YLIMRSDRGVLYMRELDLGLTLPDYF ALFKSK+GSSLA RDLILRGMKVRGEAAVRMG+
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKIGSSLAMRDLILRGMKVRGEAAVRMGI 180

Query: 181 VESTHHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPISISKL 239
           V+S HH E  V NAAV+LGEQL  R W+GEVYAEIRKSLYPE+SEVLGL GK ISISKL
Sbjct: 181 VDSAHHSEVSVVNAAVRLGEQLVGRNWDGEVYAEIRKSLYPEISEVLGLPGKAISISKL 239

BLAST of Csor.00g183290 vs. TAIR 10
Match: AT4G14430.1 (indole-3-butyric acid response 10 )

HSP 1 Score: 298.5 bits (763), Expect = 4.8e-81
Identity = 146/240 (60.83%), Postives = 191/240 (79.58%), Query Frame = 0

Query: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATSGSVLITTSHGKFFSNG 60
           MCT+EKRG+ F+LTLTGD +HR  P  I +IL  L Q K+Q+T GS+LITT++GKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGSILITTANGKFFSNG 60

Query: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120
           FDL WA  AGS +GA +RL+ +++ FKP+VA LL LPMPTIAA+ GHAAAAG  LALSHD
Sbjct: 61  FDLAWAQTAGSKTGAANRLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGLILALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGV 180
           Y+ MR DRGVLYM E+D+GL++PDYF+AL ++K+G+S A R+L+L G K+RGE AV +G+
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGEEAVGLGI 180

Query: 181 VES-THHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPISISKL 240
           V+S  +  E+GV  A+V+LGE+LAA+KW+GEVYA IRKSLYPE+  +LGL  +  +  KL
Sbjct: 181 VDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGILGLETRVFATPKL 240

BLAST of Csor.00g183290 vs. TAIR 10
Match: AT4G14440.1 (3-hydroxyacyl-CoA dehydratase 1 )

HSP 1 Score: 283.9 bits (725), Expect = 1.2e-76
Identity = 140/240 (58.33%), Postives = 180/240 (75.00%), Query Frame = 0

Query: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATSGSVLITTSHGKFFSNG 60
           MCT+EKRG+ F+LTLTG+D+HR  P  I S+L  L Q K+Q+T GSVLITT HGKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGSVLITTGHGKFFSNG 60

Query: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120
           FDL WA +AG   GA  R++ +++ FKP++A LL LPMPTIAA+ GHAAA+G   ALSHD
Sbjct: 61  FDLAWAQSAG--HGAIKRMHQMVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGV 180
           Y+ MR DRGVLYM E+D+GL +PDYF+AL  +K+GS +A R+L+L G K++GE AV +G+
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGEEAVALGI 180

Query: 181 VES-THHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPISISKL 240
           V+S  H   +GV  A V LGE LAA+KWNGEVYA IRKSLYPE+  ++ L    ++   L
Sbjct: 181 VDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMVDLTANNLATHNL 238

BLAST of Csor.00g183290 vs. TAIR 10
Match: AT1G65520.1 (delta(3), delta(2)-enoyl CoA isomerase 1 )

HSP 1 Score: 204.9 bits (520), Expect = 7.2e-53
Identity = 108/224 (48.21%), Postives = 152/224 (67.86%), Query Frame = 0

Query: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQAT-SGSVLITTSHGKFFSN 60
           MC++EKR   FIL LTGD +HRL P+L++S+   ++Q+++  + S SVLITTS GKFFSN
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSFSQSVLITTSDGKFFSN 60

Query: 61  GFDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSH 120
           G+DL  A +  S+S   D         + LVA L+SLPMPTIAA+ GHA+AAG  LA+SH
Sbjct: 61  GYDLALAESNPSLSVVMDAK------LRSLVADLISLPMPTIAAVTGHASAAGCILAMSH 120

Query: 121 DYLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMG 180
           DY++MR DRG LYM ELD+ L +P +F A+ + K+GS  A RD++L   KV  +  V+MG
Sbjct: 121 DYVLMRRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTADVGVKMG 180

Query: 181 VVESTHHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEV 224
           +V+S +        AA++LGE++  R  +G VY ++R+SL  EV
Sbjct: 181 IVDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREV 218

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O232996.7e-8060.83Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI2... [more]
O233001.7e-7558.33Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana OX=3702 GN=ECI3 PE=1 SV=1[more]
O044691.0e-5148.21Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI1... [more]
A1JK304.0e-0828.65Fatty acid oxidation complex subunit alpha OS=Yersinia enterocolitica serotype O... [more]
A7FGK17.6e-0728.81Fatty acid oxidation complex subunit alpha OS=Yersinia pseudotuberculosis seroty... [more]
Match NameE-valueIdentityDescription
KAG6582371.11.26e-164100.00Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022924234.13.48e-16298.33enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita moschata][more]
XP_023526416.15.53e-15996.65enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita pepo subsp. pepo][more]
XP_022979202.19.16e-15896.65enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita maxima][more]
KAG7018780.18.15e-14597.81Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. argyrosperma... [more]
Match NameE-valueIdentityDescription
A0A6J1E8J91.68e-16298.33enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1IN544.44e-15896.65enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A0A0L9X49.40e-14487.45Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G141770 PE=4 SV=1[more]
A0A1S3AWB31.28e-14186.61enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A5D3CZF52.58e-14186.19Enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo var. makuwa OX=119... [more]
Match NameE-valueIdentityDescription
AT4G14430.14.8e-8160.83indole-3-butyric acid response 10 [more]
AT4G14440.11.2e-7658.333-hydroxyacyl-CoA dehydratase 1 [more]
AT1G65520.17.2e-5348.21delta(3), delta(2)-enoyl CoA isomerase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001753Enoyl-CoA hydratase/isomerasePFAMPF00378ECH_1coord: 25..205
e-value: 1.4E-20
score: 73.6
NoneNo IPR availableGENE3D3.90.226.10coord: 1..227
e-value: 2.1E-36
score: 127.3
NoneNo IPR availablePANTHERPTHR11941:SF75ENOYL-COA DELTA ISOMERASE 2, PEROXISOMALcoord: 1..231
NoneNo IPR availablePANTHERPTHR11941ENOYL-COA HYDRATASE-RELATEDcoord: 1..231
NoneNo IPR availableCDDcd06558crotonase-likecoord: 2..205
e-value: 6.82361E-16
score: 71.4376
IPR029045ClpP/crotonase-like domain superfamilySUPERFAMILY52096ClpP/crotonasecoord: 3..206

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g183290.m01Csor.00g183290.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006635 fatty acid beta-oxidation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004165 dodecenoyl-CoA delta-isomerase activity
molecular_function GO:0004300 enoyl-CoA hydratase activity
molecular_function GO:0003824 catalytic activity