Csor.00g180380 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g180380
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionjasmonic acid-amido synthetase JAR1-like
LocationCsor_Chr14: 16712623 .. 16714823 (+)
RNA-Seq ExpressionCsor.00g180380
SyntenyCsor.00g180380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTGATGGTGGGAGTAGGAAGTATGGTGCCAGGTAATTTCCATGCGGGTGAGAAAAGGGAGGGTAATTGTAGTCTACTCCCAAGGGTAGAACGGTCATTATACATGTATATTGTATCTACAACTGAGTGACCCTTCAATACTAAAATCTAAAATGTTCTACAATGTTGCAGAGGATGGAAGTATTTGATGGGGAGAAAGTGATAGAGGAGTTCGAGGAAATGACGAAAGATGCGGGAAGAGTTCAGGTGGAGACTCTGAAGAAGATTTTGGAGGAAAATGGGTGGGCTGAGTACTTGCAGAATTTGGGACTTAATGGAAGAACTGATCCTCAGAGCTTCAAGGAATGCATCCCGCTTGCTACTCACGATGATTTTGAGGCATATATTCAGAGAATCGCGGATGGGGATTCTTCCCCTATTCTCACTGGAAAACCAATCGAATCAATGTCGTTGAGGTACTTTTGCAAATTCGTAGAACTGGAGATTTTGTTGTTGTTGTCGTGTTCTTCTTCTAATGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTGGTTGTACGATGTAGCTCTGGTACTAGTAAGGGGAGGCCTAAGTTCATTCCCTTCAATGATTCACTGTTTGAGACCACAATGCAAATTTTTCAAACGTCTTTTGCCTTCAGAAACAAGTAATGAAACTGTCTTTTCTGGTCTCTGAATCATTTGTTGTCATTTGTTGTCATTTGTTGCCTAAAATTGGTGTCACTACTTTCTCCTGCAGACAAATTCCTCTTGGCAATGGAAAAGCATTGCAGTTCGTCTATAGCAGCAAGCAGTTCAAAACCAAGGGTGGTCTGGCAGCAGGAGCTGCAACAACTAACGTTTACCGTAACCCGAAATTCAAAACCACGATGAAAGCAATCCAGTCGCAAAGTTGCAGCCCAGATGAGGTCATCTTTGGCTCCGACTTCCACCAATCTTTGTATTGCCATCTCTTGTGTGGGCTGATCTCCCGAGATGAAATTGAGTCGGTGTTCTCCTCTTTTGCGCACAGCATTATCCTTCCTTTCAAGACTTTTGAGCAAGTATGGGAAGAGCTCTGCACCAACATTCGAGACGGGGTTCTCTCGAGTTGGGTCACTGATCCCTCGATTCGTGCAGCCATGTCGAAATTGCTCAAGCCAGATCCTGAATTGGCTGATTTGATCTATAAAAAATGTGCAGGATTGATTAATTGGTATGGATTGATACCAGAGCTGTTTCCCAATGCCAAGTACATTTATGGGATCATGACAGGCTCGATGGAGCCTTACCTGATGAAACTGAGGCACTACGCGGGGCATTTGCCGCTGATAAGTGCCGATTATGGTGCTTCAGAAGGATGGATTGGAGTTAATGTTAACCCGATGTTGCCCCCTGAAATGGCCACCTTCGCTGTAATTCCAAACATTGGATACTTCGAGTTCATCCCGCTTAAAGCAAATGCTCAAGATCAGGAACAGGAGGGAGCGCAGGAGAACAAGCCGGTTGGTCTGACCGAAGTCAAGATCGGTGAAGAGTACGAAATAGTTGTCACCAATGTTGCAGGTAATTCCTTCCAATTTATGACATTATATACGACCTTGCTTTCAAGATATCCACAAGTGTCTGACCGAAGCGTTTATTACGGTCATTGCAGGGCTGTACCGTTATAGATTAGGAGACGTGGTGAAAGTGATGGGGTTCCACAATGCAACACCAGAGCTGAAATTTGTGTGCCGGAGCAACCTTCTGCTGAGCATCAACATGGACAAGATCACGGAGAAGGACCTGCAGCTGGCAGTGGAAGCAGCAAGGACCGTACTGGCAGCAGAGAAGCTGGAACTGGTGGAGTTCACAAGCCATGCAGACATAACAAGCGAGCCTGGGCACTATGTGATATTCTGGGAGATAAGCGGGGAGGCAAAGGAGGAGGTGCTGATAGAATGCTGCAACCGTCTGGACAAGGTGTTCCAGGAAGCGGGGTCCGTGAGCTGGAGAAAGGGGAAGGCCACTGGAGCCCTGGAGCTGAGAGTGGTCCGTAAGGGAACTTTTCATAAGATCATGGAACATTATGTGTCCTTAGGATCGGCTATGAGTCAGTACAAAACCCCTCGTGCTGTTGGCTCCACCAACACCGCCGTCTTCGACATCTTGTGCTCCAATGTTGTCAGCTCCTATTTCAGTACTGCCTTCTAA

mRNA sequence

ATGCTGATGGTGGGAGTAGGAAGTATGGTGCCAGGTAATTTCCATGCGGGTGAGAAAAGGGAGGGTAATTGTAGTCTACTCCCAAGGAGGATGGAAGTATTTGATGGGGAGAAAGTGATAGAGGAGTTCGAGGAAATGACGAAAGATGCGGGAAGAGTTCAGGTGGAGACTCTGAAGAAGATTTTGGAGGAAAATGGGTGGGCTGAGTACTTGCAGAATTTGGGACTTAATGGAAGAACTGATCCTCAGAGCTTCAAGGAATGCATCCCGCTTGCTACTCACGATGATTTTGAGGCATATATTCAGAGAATCGCGGATGGGGATTCTTCCCCTATTCTCACTGGAAAACCAATCGAATCAATGTCGTTGAGCTCTGGTACTAGTAAGGGGAGGCCTAAGTTCATTCCCTTCAATGATTCACTGTTTGAGACCACAATGCAAATTTTTCAAACGTCTTTTGCCTTCAGAAACAAACAAATTCCTCTTGGCAATGGAAAAGCATTGCAGTTCGTCTATAGCAGCAAGCAGTTCAAAACCAAGGGTGGTCTGGCAGCAGGAGCTGCAACAACTAACGTTTACCGTAACCCGAAATTCAAAACCACGATGAAAGCAATCCAGTCGCAAAGTTGCAGCCCAGATGAGGTCATCTTTGGCTCCGACTTCCACCAATCTTTGTATTGCCATCTCTTGTGTGGGCTGATCTCCCGAGATGAAATTGAGTCGGTGTTCTCCTCTTTTGCGCACAGCATTATCCTTCCTTTCAAGACTTTTGAGCAAGTATGGGAAGAGCTCTGCACCAACATTCGAGACGGGGTTCTCTCGAGTTGGGTCACTGATCCCTCGATTCGTGCAGCCATGTCGAAATTGCTCAAGCCAGATCCTGAATTGGCTGATTTGATCTATAAAAAATGTGCAGGATTGATTAATTGGTATGGATTGATACCAGAGCTGTTTCCCAATGCCAAGTACATTTATGGGATCATGACAGGCTCGATGGAGCCTTACCTGATGAAACTGAGGCACTACGCGGGGCATTTGCCGCTGATAAGTGCCGATTATGGTGCTTCAGAAGGATGGATTGGAGTTAATGTTAACCCGATGTTGCCCCCTGAAATGGCCACCTTCGCTGTAATTCCAAACATTGGATACTTCGAGTTCATCCCGCTTAAAGCAAATGCTCAAGATCAGGAACAGGAGGGAGCGCAGGAGAACAAGCCGGTTGGTCTGACCGAAGTCAAGATCGGTGAAGAGTACGAAATAGTTGTCACCAATGTTGCAGGGCTGTACCGTTATAGATTAGGAGACGTGGTGAAAGTGATGGGGTTCCACAATGCAACACCAGAGCTGAAATTTGTGTGCCGGAGCAACCTTCTGCTGAGCATCAACATGGACAAGATCACGGAGAAGGACCTGCAGCTGGCAGTGGAAGCAGCAAGGACCGTACTGGCAGCAGAGAAGCTGGAACTGGTGGAGTTCACAAGCCATGCAGACATAACAAGCGAGCCTGGGCACTATGTGATATTCTGGGAGATAAGCGGGGAGGCAAAGGAGGAGGTGCTGATAGAATGCTGCAACCGTCTGGACAAGGTGTTCCAGGAAGCGGGGTCCGTGAGCTGGAGAAAGGGGAAGGCCACTGGAGCCCTGGAGCTGAGAGTGGTCCGTAAGGGAACTTTTCATAAGATCATGGAACATTATGTGTCCTTAGGATCGGCTATGAGTCAGTACAAAACCCCTCGTGCTGTTGGCTCCACCAACACCGCCGTCTTCGACATCTTGTGCTCCAATGTTGTCAGCTCCTATTTCAGTACTGCCTTCTAA

Coding sequence (CDS)

ATGCTGATGGTGGGAGTAGGAAGTATGGTGCCAGGTAATTTCCATGCGGGTGAGAAAAGGGAGGGTAATTGTAGTCTACTCCCAAGGAGGATGGAAGTATTTGATGGGGAGAAAGTGATAGAGGAGTTCGAGGAAATGACGAAAGATGCGGGAAGAGTTCAGGTGGAGACTCTGAAGAAGATTTTGGAGGAAAATGGGTGGGCTGAGTACTTGCAGAATTTGGGACTTAATGGAAGAACTGATCCTCAGAGCTTCAAGGAATGCATCCCGCTTGCTACTCACGATGATTTTGAGGCATATATTCAGAGAATCGCGGATGGGGATTCTTCCCCTATTCTCACTGGAAAACCAATCGAATCAATGTCGTTGAGCTCTGGTACTAGTAAGGGGAGGCCTAAGTTCATTCCCTTCAATGATTCACTGTTTGAGACCACAATGCAAATTTTTCAAACGTCTTTTGCCTTCAGAAACAAACAAATTCCTCTTGGCAATGGAAAAGCATTGCAGTTCGTCTATAGCAGCAAGCAGTTCAAAACCAAGGGTGGTCTGGCAGCAGGAGCTGCAACAACTAACGTTTACCGTAACCCGAAATTCAAAACCACGATGAAAGCAATCCAGTCGCAAAGTTGCAGCCCAGATGAGGTCATCTTTGGCTCCGACTTCCACCAATCTTTGTATTGCCATCTCTTGTGTGGGCTGATCTCCCGAGATGAAATTGAGTCGGTGTTCTCCTCTTTTGCGCACAGCATTATCCTTCCTTTCAAGACTTTTGAGCAAGTATGGGAAGAGCTCTGCACCAACATTCGAGACGGGGTTCTCTCGAGTTGGGTCACTGATCCCTCGATTCGTGCAGCCATGTCGAAATTGCTCAAGCCAGATCCTGAATTGGCTGATTTGATCTATAAAAAATGTGCAGGATTGATTAATTGGTATGGATTGATACCAGAGCTGTTTCCCAATGCCAAGTACATTTATGGGATCATGACAGGCTCGATGGAGCCTTACCTGATGAAACTGAGGCACTACGCGGGGCATTTGCCGCTGATAAGTGCCGATTATGGTGCTTCAGAAGGATGGATTGGAGTTAATGTTAACCCGATGTTGCCCCCTGAAATGGCCACCTTCGCTGTAATTCCAAACATTGGATACTTCGAGTTCATCCCGCTTAAAGCAAATGCTCAAGATCAGGAACAGGAGGGAGCGCAGGAGAACAAGCCGGTTGGTCTGACCGAAGTCAAGATCGGTGAAGAGTACGAAATAGTTGTCACCAATGTTGCAGGGCTGTACCGTTATAGATTAGGAGACGTGGTGAAAGTGATGGGGTTCCACAATGCAACACCAGAGCTGAAATTTGTGTGCCGGAGCAACCTTCTGCTGAGCATCAACATGGACAAGATCACGGAGAAGGACCTGCAGCTGGCAGTGGAAGCAGCAAGGACCGTACTGGCAGCAGAGAAGCTGGAACTGGTGGAGTTCACAAGCCATGCAGACATAACAAGCGAGCCTGGGCACTATGTGATATTCTGGGAGATAAGCGGGGAGGCAAAGGAGGAGGTGCTGATAGAATGCTGCAACCGTCTGGACAAGGTGTTCCAGGAAGCGGGGTCCGTGAGCTGGAGAAAGGGGAAGGCCACTGGAGCCCTGGAGCTGAGAGTGGTCCGTAAGGGAACTTTTCATAAGATCATGGAACATTATGTGTCCTTAGGATCGGCTATGAGTCAGTACAAAACCCCTCGTGCTGTTGGCTCCACCAACACCGCCGTCTTCGACATCTTGTGCTCCAATGTTGTCAGCTCCTATTTCAGTACTGCCTTCTAA

Protein sequence

MLMVGVGSMVPGNFHAGEKREGNCSLLPRRMEVFDGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGRTDPQSFKECIPLATHDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETTMQIFQTSFAFRNKQIPLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQSQSCSPDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELCTNIRDGVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIYGIMTGSMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEFIPLKANAQDQEQEGAQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNATPELKFVCRSNLLLSINMDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPGHYVIFWEISGEAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHYVSLGSAMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF
Homology
BLAST of Csor.00g180380 vs. ExPASy Swiss-Prot
Match: A0A314KSQ4 (Jasmonoyl--L-amino acid synthetase JAR6 OS=Nicotiana attenuata OX=49451 GN=JAR6 PE=1 SV=2)

HSP 1 Score: 869.4 bits (2245), Expect = 2.4e-251
Identity = 418/572 (73.08%), Postives = 487/572 (85.14%), Query Frame = 0

Query: 34  FDGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGRTDPQSFKECIPLAT 93
           FD EKVIEEFE++TKDAG++Q ETLKKILE+NG  EYLQ  GLNGRTDPQ+FK C+P+ T
Sbjct: 12  FDQEKVIEEFEDLTKDAGKIQEETLKKILEQNGGTEYLQLWGLNGRTDPQTFKNCVPIVT 71

Query: 94  HDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETTMQIFQTSF 153
           H+D E YIQRIADGD SPILTGKPIE++SLSSGT++G+PKF+PFND L E+TMQIF+TSF
Sbjct: 72  HNDLEPYIQRIADGDLSPILTGKPIETISLSSGTTQGKPKFVPFNDELMESTMQIFKTSF 131

Query: 154 AFRNKQIPLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQSQSCSPD 213
           AFRN++ P+GNGKALQF+YSSKQFKTKGGLAAG ATTNVYRN +FK TMKA+ +  CSPD
Sbjct: 132 AFRNREFPIGNGKALQFIYSSKQFKTKGGLAAGTATTNVYRNAQFKKTMKAMCTPCCSPD 191

Query: 214 EVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELCTNIRDGVL 273
           EVIFG DFHQSLYCHLLCGLI  DE++ V S+FAHSI+  F+TFEQVWE L  +IR+GVL
Sbjct: 192 EVIFGPDFHQSLYCHLLCGLIFHDEVQVVSSTFAHSIVHAFRTFEQVWEALVVDIREGVL 251

Query: 274 SSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIYGIMTGSME 333
           SS VT PSIR AMSKLLKPDPELAD IY KC+ L NWYGLIP+LFPN +YIYGIMTGSME
Sbjct: 252 SSRVTVPSIRLAMSKLLKPDPELADTIYNKCSRLSNWYGLIPDLFPNTRYIYGIMTGSME 311

Query: 334 PYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEFIPLKANA 393
           PYL KLRHYAG LPL+SADYG+SEGW+GVNVNP LPPE+ T+AV+PNIGYFEFIPL  N 
Sbjct: 312 PYLKKLRHYAGELPLLSADYGSSEGWVGVNVNPKLPPELVTYAVLPNIGYFEFIPLGGNL 371

Query: 394 QDQEQEGAQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNATPELKFVC 453
              E    Q N PVGLTEVK+GEEYE+V TN AGLYRYRLGDVVKV GFHN TPEL+FVC
Sbjct: 372 NGIE----QANSPVGLTEVKLGEEYEVVFTNFAGLYRYRLGDVVKVKGFHNGTPELQFVC 431

Query: 454 RSNLLLSINMDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPGHYVIFWEISG 513
           RSNLLLSIN+DK TEKDLQLAVEAA   L  EKLE+V+FTSH +++++PGHYVIFWE+SG
Sbjct: 432 RSNLLLSINIDKNTEKDLQLAVEAAAKRLVDEKLEVVDFTSHVNVSADPGHYVIFWELSG 491

Query: 514 EAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHYVSLGSAMS 573
           EA +E+L +CCN LD+ F +AG VS RK  A GALELR+V++GTFHKI++H+V LG A+S
Sbjct: 492 EATDEMLQDCCNCLDRSFIDAGYVSSRKVNAIGALELRIVKRGTFHKILDHFVGLGGAVS 551

Query: 574 QYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF 606
           Q+KTPR VG  N+++  IL SNVV +Y STAF
Sbjct: 552 QFKTPRCVGPKNSSLLQILSSNVVETYVSTAF 579

BLAST of Csor.00g180380 vs. ExPASy Swiss-Prot
Match: A0A1J6KGJ9 (Jasmonoyl--L-amino acid synthetase JAR4 OS=Nicotiana attenuata OX=49451 GN=JAR4 PE=1 SV=1)

HSP 1 Score: 857.8 bits (2215), Expect = 7.2e-248
Identity = 413/580 (71.21%), Postives = 484/580 (83.45%), Query Frame = 0

Query: 26  LLPRRMEVFDGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGRTDPQSF 85
           ++  + E FD E+VIEEFE +TKDAG++Q ETL+KILEENG  EYLQ  GLNG+TD  SF
Sbjct: 3   MVVEKTEKFDPEEVIEEFEVLTKDAGKIQEETLQKILEENGGTEYLQQWGLNGKTDSLSF 62

Query: 86  KECIPLATHDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETT 145
           K CIP+ TH D E YI RIADGD SPILTGKPI ++SLSSGT++G+PKF+PFN+ L E+T
Sbjct: 63  KNCIPIVTHKDLEPYIHRIADGDLSPILTGKPITTISLSSGTTQGKPKFVPFNEELMEST 122

Query: 146 MQIFQTSFAFRNKQIPLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAI 205
           MQIF+TSF FRN++ P+ NGKALQF+Y SKQFKTKGGLAAG ATTNVYRN +FK TMKA+
Sbjct: 123 MQIFKTSFVFRNREFPVVNGKALQFIYGSKQFKTKGGLAAGTATTNVYRNAQFKKTMKAM 182

Query: 206 QSQSCSPDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELC 265
           Q+  CSPDEVIFG DF QSLYCHLLCGLI RDE++ V S+FAHSI+  F+ FEQ+W+EL 
Sbjct: 183 QTPCCSPDEVIFGPDFQQSLYCHLLCGLIFRDEVQVVSSTFAHSIVHAFRNFEQIWQELV 242

Query: 266 TNIRDGVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIY 325
           TNIR+GVLSS V  PS+RAAMSKLLKPDPELAD I+ KC+ L NWYGLIPELFPN +YIY
Sbjct: 243 TNIREGVLSSRVIVPSMRAAMSKLLKPDPELADTIFNKCSRLSNWYGLIPELFPNTRYIY 302

Query: 326 GIMTGSMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFE 385
           GIMTGSMEPYL KLRHYAG LPL+SADYG+SEGWIG NVNP LPPE+ T+AV+PNIGYFE
Sbjct: 303 GIMTGSMEPYLKKLRHYAGDLPLLSADYGSSEGWIGANVNPELPPELVTYAVLPNIGYFE 362

Query: 386 FIPLKANAQDQEQEGAQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNA 445
           FIPL  N          E  PVGLTEVK+GEEYEIVVTN AGLYRYRLGDVVK+ GFHN 
Sbjct: 363 FIPLMENLD------GLEPMPVGLTEVKLGEEYEIVVTNFAGLYRYRLGDVVKIKGFHNG 422

Query: 446 TPELKFVCRSNLLLSINMDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPGHY 505
           TPEL+F+CR NLLLSIN+DK TEKDLQLAVEAA  +L+ EKLE+V+FTSH +++++PGHY
Sbjct: 423 TPELQFICRRNLLLSINIDKNTEKDLQLAVEAAAKILSDEKLEVVDFTSHVNVSADPGHY 482

Query: 506 VIFWEISGEAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHY 565
           VIFWE++GEA EE+L ECCN LDK F +AG V  RK  A GALELR+V++GTFHKI++H+
Sbjct: 483 VIFWELNGEASEEILKECCNCLDKSFVDAGYVGSRKVHAIGALELRIVKRGTFHKILDHF 542

Query: 566 VSLGSAMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF 606
           V LG+A+SQ+KTPR VG TN +V  IL SNVV SYFSTAF
Sbjct: 543 VGLGAAVSQFKTPRCVGPTNLSVLQILSSNVVESYFSTAF 576

BLAST of Csor.00g180380 vs. ExPASy Swiss-Prot
Match: Q6I581 (Jasmonoyl--L-amino acid synthetase GH3.5 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 785.4 bits (2027), Expect = 4.6e-226
Identity = 374/579 (64.59%), Postives = 463/579 (79.97%), Query Frame = 0

Query: 31  MEVFDGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGRTDPQSFKECIP 90
           M +   E+ I EFE +T+DA RVQ +TLKKILE N  AEYLQN GL GRTD +S+K CIP
Sbjct: 1   MTICSCEETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNFGLGGRTDAESYKSCIP 60

Query: 91  LATHDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETTMQIFQ 150
           L  H+D E YIQRI DGD+SP++TG+PI ++SLSSGT+ G+PKFIPFND L ETT+QI++
Sbjct: 61  LCVHNDIEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKPKFIPFNDELLETTLQIYR 120

Query: 151 TSFAFRNKQIPLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQSQSC 210
           TS+AFRN++ P+G GKALQFVY SKQ  TKGG+ A  ATTN+YR  ++K  MK IQSQ C
Sbjct: 121 TSYAFRNREYPIGQGKALQFVYGSKQVITKGGILATTATTNLYRRQRYKEGMKDIQSQCC 180

Query: 211 SPDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELCTNIRD 270
           SPDEVIFG DFHQSLYCHLLCGLI  +E+ SVFS+FAHS++  F+TFE+VWE+LCT+IRD
Sbjct: 181 SPDEVIFGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAHSLVHAFQTFEEVWEDLCTDIRD 240

Query: 271 GVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIYGIMTG 330
           GVLS  VT PSIR A+SK+LKP+PELAD IYKKC GL NWYG+IP L+PNAKY+YGIMTG
Sbjct: 241 GVLSKKVTAPSIREAVSKILKPNPELADSIYKKCIGLSNWYGVIPALWPNAKYVYGIMTG 300

Query: 331 SMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEFIPLK 390
           SMEPYL KLRHYAG+LPLISADYGASEGW+G N++P +PPE  T+AV+P +GYFEFIPL+
Sbjct: 301 SMEPYLKKLRHYAGNLPLISADYGASEGWVGSNIDPTVPPEQVTYAVLPQVGYFEFIPLE 360

Query: 391 ANAQDQEQEGAQ----ENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNAT 450
               ++ +  A     E+ PVGLTEV++G+ YE+V+TN AGLYRYRLGDVVK+  FHN+T
Sbjct: 361 KPIGEETENSASIHYIESDPVGLTEVEVGKIYEVVITNFAGLYRYRLGDVVKIARFHNST 420

Query: 451 PELKFVCRSNLLLSINMDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPGHYV 510
           PEL+F+CR +L+LSIN+DK TEKDLQLAVE A   L  EKLE+++FTS  + +S+PG YV
Sbjct: 421 PELQFICRRSLVLSINIDKNTEKDLQLAVEEASKFLEGEKLEVMDFTSFVERSSDPGRYV 480

Query: 511 IFWEISGEAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHYV 570
           IFWE+SG+A +EVL  C N LD  F +AG    RK K  G LELR++RKGTF +I++H++
Sbjct: 481 IFWELSGDASDEVLSSCANALDLAFIDAGYTGSRKIKTIGPLELRILRKGTFKEILDHFL 540

Query: 571 SLGSAMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF 606
           SLG A+SQ+KTPR V  +N+ V  IL  NV  SYFSTA+
Sbjct: 541 SLGGAVSQFKTPRFVNPSNSKVLQILSRNVTQSYFSTAY 579

BLAST of Csor.00g180380 vs. ExPASy Swiss-Prot
Match: Q9SKE2 (Jasmonoyl--L-amino acid synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 PE=1 SV=2)

HSP 1 Score: 758.8 bits (1958), Expect = 4.6e-218
Identity = 367/579 (63.39%), Postives = 459/579 (79.27%), Query Frame = 0

Query: 30  RMEVFDGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGR-TDP-QSFKE 89
           ++E FD  +VI+EF+EMT++A +VQ +TLK+IL +N  A YLQN GLNG  TDP ++FK 
Sbjct: 4   KVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATDPEEAFKS 63

Query: 90  CIPLATHDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETTMQ 149
            +PL T  + E YI+R+ DGD+SPILTG P+ ++SLSSGTS+GRPKFIPF D L E T+Q
Sbjct: 64  MVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQ 123

Query: 150 IFQTSFAFRNKQIPL-GNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQ 209
           +F+T+FAFRN+  P+  NGKALQF++SSKQ+ + GG+  G ATTNVYRNP FK  MK+I 
Sbjct: 124 LFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSIT 183

Query: 210 SQSCSPDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELCT 269
           S SCSPDEVIF  D HQ+LYCHLL G++ RD+++ VF+ FAH ++  F+TFEQVWEE+ T
Sbjct: 184 SPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIVT 243

Query: 270 NIRDGVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIYG 329
           +I+DGVLS+ +T PS+R AMSKLL P+PELA+ I  KC  L NWYGLIP LFPNAKY+YG
Sbjct: 244 DIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPNAKYVYG 303

Query: 330 IMTGSMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEF 389
           IMTGSMEPY+ KLRHYAG LPL+S DYG+SEGWI  NV P L PE ATFAVIPN+GYFEF
Sbjct: 304 IMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGYFEF 363

Query: 390 IPLKANAQDQEQEGAQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNAT 449
           +P+        + G  E KPVGLT+VKIGEEYE+V+TN AGLYRYRLGDVVKV+GF+N T
Sbjct: 364 LPV-------SETGEGEEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKVIGFYNNT 423

Query: 450 PELKFVCRSNLLLSINMDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPGHYV 509
           P+LKF+CR NL+LSIN+DK TE+DLQL+VE+A   L+ EK+E+++F+S+ D++++PGHY 
Sbjct: 424 PQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEKIEVIDFSSYIDVSTDPGHYA 483

Query: 510 IFWEISGEAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHYV 569
           IFWEISGE  E+VL +CCN LD+ F +AG VS RK K  GALELRVV KGTF KI EH++
Sbjct: 484 IFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTFRKIQEHFL 543

Query: 570 SLGSAMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF 606
            LGS+  Q+K PR V  +N  V  ILC NVVSSYFSTAF
Sbjct: 544 GLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

BLAST of Csor.00g180380 vs. ExPASy Swiss-Prot
Match: Q53P49 (Probable indole-3-acetic acid-amido synthetase GH3.12 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.12 PE=2 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 2.6e-165
Identity = 290/598 (48.49%), Postives = 408/598 (68.23%), Query Frame = 0

Query: 35  DGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGRTDPQSFKECIPLATH 94
           + E+++  FE  T+DA  VQ ETL++IL EN   EYL+ LGL G TD  SF+  +P+ TH
Sbjct: 13  EDEEIMAWFERTTRDAADVQRETLRRILAENAGVEYLRELGLAGLTDAGSFRARVPVVTH 72

Query: 95  DDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETTMQIFQTSFA 154
            D + YIQR+ADGD+SP+LT KP+ ++SLSSGT++G+ K + FND L  ++++ F  S+A
Sbjct: 73  ADLDPYIQRVADGDASPVLTAKPVTAISLSSGTTQGKRKRLLFNDDLLRSSIRFFHASYA 132

Query: 155 FRNKQIPLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKA---IQSQSCS 214
           F N+  P+ +G+ LQF+Y S+   TKGGL A    TN+ R+ +F  +M A    +  SCS
Sbjct: 133 FTNRAFPVEDGRVLQFMYGSRHETTKGGLTATTVMTNLLRSEEFTASMAARSRPRLPSCS 192

Query: 215 PDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELCTNIRDG 274
           P EV+F  DF +SLYCHLLCGL+   E+ +V +SFAHSI++  +  E+VW ELC +IR G
Sbjct: 193 PSEVVFSPDFDESLYCHLLCGLLLAGEVRAVSASFAHSIVVALQALERVWRELCADIRRG 252

Query: 275 VLS-SWVTDPSIRAAMSKLL-KPDPELADLIYKKCAGLINWYGLIPELFPNAKYIYGIMT 334
             S + VT P++R A++ +L  P+P LAD + ++CA L +W G+IP L+PNA+Y+   MT
Sbjct: 253 AASPARVTTPAVRRAVAPILAAPNPALADALERRCAALGDWSGVIPALWPNARYVQATMT 312

Query: 335 GSMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEFIPL 394
           GSME Y+ KLRHYAG +PL+S +Y +SEG IG+N     PPE   F V+P+  YFEFIPL
Sbjct: 313 GSMEHYVKKLRHYAGGVPLVSGNYASSEGVIGINAEQHAPPESVVFTVLPDAAYFEFIPL 372

Query: 395 KANAQDQEQE-------------GAQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDV 454
           K    D   +              A +  PVGLT+V +GE YE+V+T   GLYRYRLGDV
Sbjct: 373 KPPCTDAAADDDNPAAAGSSCYVDADDANPVGLTDVVVGEHYEVVMTTFTGLYRYRLGDV 432

Query: 455 VKVMGFHNATPELKFVCRSNLLLSINMDKITEKDLQLAVEAARTVLAAE-----KLELVE 514
           VKV GFH+ATP+L+FVCR +L+LSIN+DK +E DLQLAV++A  +LA +     +LE+ +
Sbjct: 433 VKVAGFHHATPKLRFVCRRSLILSINVDKNSEHDLQLAVDSAAKILAGDGENHKQLEIAD 492

Query: 515 FTSHADITSEPGHYVIFWEISGEAKEE---VLIECCNRLDKVF-QEAGSVSWRKGKATGA 574
           +TSHAD +S+PGHYV+FWE++G  +E+   VL  CC+ +D+ F  +AG    RK  A GA
Sbjct: 493 YTSHADTSSDPGHYVVFWELNGGGEEDGGGVLQRCCDEMDRAFGADAGYAQSRKTCAIGA 552

Query: 575 LELRVVRKGTFHKIMEHYVSLGSAMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF 606
           LELRV+R+G F +++ HYV+ GS+  Q+K PR V  +N  V  +L  N ++ +FSTA+
Sbjct: 553 LELRVLRRGAFQEVLRHYVAGGSSAGQFKMPRCVAPSNAGVLRVLKDNTINIFFSTAY 610

BLAST of Csor.00g180380 vs. NCBI nr
Match: KAG6582660.1 (Jasmonoyl--L-amino acid synthetase JAR6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1215 bits (3144), Expect = 0.0
Identity = 605/605 (100.00%), Postives = 605/605 (100.00%), Query Frame = 0

Query: 1   MLMVGVGSMVPGNFHAGEKREGNCSLLPRRMEVFDGEKVIEEFEEMTKDAGRVQVETLKK 60
           MLMVGVGSMVPGNFHAGEKREGNCSLLPRRMEVFDGEKVIEEFEEMTKDAGRVQVETLKK
Sbjct: 1   MLMVGVGSMVPGNFHAGEKREGNCSLLPRRMEVFDGEKVIEEFEEMTKDAGRVQVETLKK 60

Query: 61  ILEENGWAEYLQNLGLNGRTDPQSFKECIPLATHDDFEAYIQRIADGDSSPILTGKPIES 120
           ILEENGWAEYLQNLGLNGRTDPQSFKECIPLATHDDFEAYIQRIADGDSSPILTGKPIES
Sbjct: 61  ILEENGWAEYLQNLGLNGRTDPQSFKECIPLATHDDFEAYIQRIADGDSSPILTGKPIES 120

Query: 121 MSLSSGTSKGRPKFIPFNDSLFETTMQIFQTSFAFRNKQIPLGNGKALQFVYSSKQFKTK 180
           MSLSSGTSKGRPKFIPFNDSLFETTMQIFQTSFAFRNKQIPLGNGKALQFVYSSKQFKTK
Sbjct: 121 MSLSSGTSKGRPKFIPFNDSLFETTMQIFQTSFAFRNKQIPLGNGKALQFVYSSKQFKTK 180

Query: 181 GGLAAGAATTNVYRNPKFKTTMKAIQSQSCSPDEVIFGSDFHQSLYCHLLCGLISRDEIE 240
           GGLAAGAATTNVYRNPKFKTTMKAIQSQSCSPDEVIFGSDFHQSLYCHLLCGLISRDEIE
Sbjct: 181 GGLAAGAATTNVYRNPKFKTTMKAIQSQSCSPDEVIFGSDFHQSLYCHLLCGLISRDEIE 240

Query: 241 SVFSSFAHSIILPFKTFEQVWEELCTNIRDGVLSSWVTDPSIRAAMSKLLKPDPELADLI 300
           SVFSSFAHSIILPFKTFEQVWEELCTNIRDGVLSSWVTDPSIRAAMSKLLKPDPELADLI
Sbjct: 241 SVFSSFAHSIILPFKTFEQVWEELCTNIRDGVLSSWVTDPSIRAAMSKLLKPDPELADLI 300

Query: 301 YKKCAGLINWYGLIPELFPNAKYIYGIMTGSMEPYLMKLRHYAGHLPLISADYGASEGWI 360
           YKKCAGLINWYGLIPELFPNAKYIYGIMTGSMEPYLMKLRHYAGHLPLISADYGASEGWI
Sbjct: 301 YKKCAGLINWYGLIPELFPNAKYIYGIMTGSMEPYLMKLRHYAGHLPLISADYGASEGWI 360

Query: 361 GVNVNPMLPPEMATFAVIPNIGYFEFIPLKANAQDQEQEGAQENKPVGLTEVKIGEEYEI 420
           GVNVNPMLPPEMATFAVIPNIGYFEFIPLKANAQDQEQEGAQENKPVGLTEVKIGEEYEI
Sbjct: 361 GVNVNPMLPPEMATFAVIPNIGYFEFIPLKANAQDQEQEGAQENKPVGLTEVKIGEEYEI 420

Query: 421 VVTNVAGLYRYRLGDVVKVMGFHNATPELKFVCRSNLLLSINMDKITEKDLQLAVEAART 480
           VVTNVAGLYRYRLGDVVKVMGFHNATPELKFVCRSNLLLSINMDKITEKDLQLAVEAART
Sbjct: 421 VVTNVAGLYRYRLGDVVKVMGFHNATPELKFVCRSNLLLSINMDKITEKDLQLAVEAART 480

Query: 481 VLAAEKLELVEFTSHADITSEPGHYVIFWEISGEAKEEVLIECCNRLDKVFQEAGSVSWR 540
           VLAAEKLELVEFTSHADITSEPGHYVIFWEISGEAKEEVLIECCNRLDKVFQEAGSVSWR
Sbjct: 481 VLAAEKLELVEFTSHADITSEPGHYVIFWEISGEAKEEVLIECCNRLDKVFQEAGSVSWR 540

Query: 541 KGKATGALELRVVRKGTFHKIMEHYVSLGSAMSQYKTPRAVGSTNTAVFDILCSNVVSSY 600
           KGKATGALELRVVRKGTFHKIMEHYVSLGSAMSQYKTPRAVGSTNTAVFDILCSNVVSSY
Sbjct: 541 KGKATGALELRVVRKGTFHKIMEHYVSLGSAMSQYKTPRAVGSTNTAVFDILCSNVVSSY 600

Query: 601 FSTAF 605
           FSTAF
Sbjct: 601 FSTAF 605

BLAST of Csor.00g180380 vs. NCBI nr
Match: KAG7019058.1 (Jasmonic acid-amido synthetase JAR1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1157 bits (2992), Expect = 0.0
Identity = 577/580 (99.48%), Postives = 579/580 (99.83%), Query Frame = 0

Query: 26   LLPRRMEVFDGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGRTDPQSF 85
            +L +RMEVFDGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGRTDPQSF
Sbjct: 563  VLFQRMEVFDGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGRTDPQSF 622

Query: 86   KECIPLATHDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETT 145
            KECIPLATHDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETT
Sbjct: 623  KECIPLATHDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETT 682

Query: 146  MQIFQTSFAFRNKQIPLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAI 205
            MQIFQTSFAFRNKQIPLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAI
Sbjct: 683  MQIFQTSFAFRNKQIPLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAI 742

Query: 206  QSQSCSPDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELC 265
            QSQSCSPDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELC
Sbjct: 743  QSQSCSPDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELC 802

Query: 266  TNIRDGVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIY 325
            TNIRDGVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIY
Sbjct: 803  TNIRDGVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIY 862

Query: 326  GIMTGSMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFE 385
            GIMTGSMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFE
Sbjct: 863  GIMTGSMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFE 922

Query: 386  FIPLKANAQDQEQEGAQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNA 445
            FIPLKANAQDQEQEGAQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNA
Sbjct: 923  FIPLKANAQDQEQEGAQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNA 982

Query: 446  TPELKFVCRSNLLLSINMDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPGHY 505
            TPELKFVCRSNLLLSINMDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPGHY
Sbjct: 983  TPELKFVCRSNLLLSINMDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPGHY 1042

Query: 506  VIFWEISGEAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHY 565
            VIFWEISGEAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHY
Sbjct: 1043 VIFWEISGEAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHY 1102

Query: 566  VSLGSAMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF 605
            VSLGSAMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF
Sbjct: 1103 VSLGSAMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF 1142

BLAST of Csor.00g180380 vs. NCBI nr
Match: XP_022924607.1 (jasmonic acid-amido synthetase JAR1-like isoform X1 [Cucurbita moschata] >XP_022924608.1 jasmonic acid-amido synthetase JAR1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1156 bits (2990), Expect = 0.0
Identity = 576/577 (99.83%), Postives = 577/577 (100.00%), Query Frame = 0

Query: 29  RRMEVFDGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGRTDPQSFKEC 88
           +RMEVFDGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGRTDPQSFKEC
Sbjct: 3   QRMEVFDGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGRTDPQSFKEC 62

Query: 89  IPLATHDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETTMQI 148
           IPLATHDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETTMQI
Sbjct: 63  IPLATHDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETTMQI 122

Query: 149 FQTSFAFRNKQIPLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQSQ 208
           FQTSFAFRNKQIPLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQSQ
Sbjct: 123 FQTSFAFRNKQIPLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQSQ 182

Query: 209 SCSPDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELCTNI 268
           SCSPDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELCTNI
Sbjct: 183 SCSPDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELCTNI 242

Query: 269 RDGVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIYGIM 328
           RDGVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIYGIM
Sbjct: 243 RDGVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIYGIM 302

Query: 329 TGSMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEFIP 388
           TGSMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEFIP
Sbjct: 303 TGSMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEFIP 362

Query: 389 LKANAQDQEQEGAQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNATPE 448
           LKANAQDQEQEGAQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNATPE
Sbjct: 363 LKANAQDQEQEGAQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNATPE 422

Query: 449 LKFVCRSNLLLSINMDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPGHYVIF 508
           LKFVCRSNLLLSINMDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPGHYVIF
Sbjct: 423 LKFVCRSNLLLSINMDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPGHYVIF 482

Query: 509 WEISGEAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHYVSL 568
           WEISGEAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHYVSL
Sbjct: 483 WEISGEAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHYVSL 542

Query: 569 GSAMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF 605
           GSAMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF
Sbjct: 543 GSAMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF 579

BLAST of Csor.00g180380 vs. NCBI nr
Match: XP_022924609.1 (jasmonic acid-amido synthetase JAR1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1154 bits (2984), Expect = 0.0
Identity = 575/575 (100.00%), Postives = 575/575 (100.00%), Query Frame = 0

Query: 31  MEVFDGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGRTDPQSFKECIP 90
           MEVFDGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGRTDPQSFKECIP
Sbjct: 1   MEVFDGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGRTDPQSFKECIP 60

Query: 91  LATHDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETTMQIFQ 150
           LATHDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETTMQIFQ
Sbjct: 61  LATHDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETTMQIFQ 120

Query: 151 TSFAFRNKQIPLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQSQSC 210
           TSFAFRNKQIPLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQSQSC
Sbjct: 121 TSFAFRNKQIPLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQSQSC 180

Query: 211 SPDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELCTNIRD 270
           SPDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELCTNIRD
Sbjct: 181 SPDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELCTNIRD 240

Query: 271 GVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIYGIMTG 330
           GVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIYGIMTG
Sbjct: 241 GVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIYGIMTG 300

Query: 331 SMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEFIPLK 390
           SMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEFIPLK
Sbjct: 301 SMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEFIPLK 360

Query: 391 ANAQDQEQEGAQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNATPELK 450
           ANAQDQEQEGAQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNATPELK
Sbjct: 361 ANAQDQEQEGAQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNATPELK 420

Query: 451 FVCRSNLLLSINMDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPGHYVIFWE 510
           FVCRSNLLLSINMDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPGHYVIFWE
Sbjct: 421 FVCRSNLLLSINMDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPGHYVIFWE 480

Query: 511 ISGEAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHYVSLGS 570
           ISGEAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHYVSLGS
Sbjct: 481 ISGEAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHYVSLGS 540

Query: 571 AMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF 605
           AMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF
Sbjct: 541 AMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF 575

BLAST of Csor.00g180380 vs. NCBI nr
Match: XP_023527408.1 (jasmonic acid-amido synthetase JAR1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1125 bits (2911), Expect = 0.0
Identity = 560/576 (97.22%), Postives = 567/576 (98.44%), Query Frame = 0

Query: 30  RMEVFDGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGRTDPQSFKECI 89
           RMEVFDGEKV+EEFEEMTKDA RVQVETLKKILEENG AEYLQNLGLNGRTDPQSFKEC+
Sbjct: 6   RMEVFDGEKVVEEFEEMTKDAERVQVETLKKILEENGSAEYLQNLGLNGRTDPQSFKECV 65

Query: 90  PLATHDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETTMQIF 149
           PLATHDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETTMQIF
Sbjct: 66  PLATHDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETTMQIF 125

Query: 150 QTSFAFRNKQIPLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQSQS 209
           QTSFAFRNKQ+PLGNGKALQFVYSSKQFKTKGGL+AGAATTNVYRNPKFKTTMKAIQSQS
Sbjct: 126 QTSFAFRNKQVPLGNGKALQFVYSSKQFKTKGGLSAGAATTNVYRNPKFKTTMKAIQSQS 185

Query: 210 CSPDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELCTNIR 269
           CSPDEVIFGSDFHQSLYCHLLCGL+SRDEIESVFSSFAHSIIL F+TFE VWEELCTNIR
Sbjct: 186 CSPDEVIFGSDFHQSLYCHLLCGLVSRDEIESVFSSFAHSIILAFRTFEHVWEELCTNIR 245

Query: 270 DGVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIYGIMT 329
           DGVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGL NWYGLIPELFPNAKYIYGIMT
Sbjct: 246 DGVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLSNWYGLIPELFPNAKYIYGIMT 305

Query: 330 GSMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEFIPL 389
           GSMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEFIPL
Sbjct: 306 GSMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEFIPL 365

Query: 390 KANAQDQEQEGAQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNATPEL 449
           KANAQDQEQEGA ENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNATPEL
Sbjct: 366 KANAQDQEQEGAPENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNATPEL 425

Query: 450 KFVCRSNLLLSINMDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPGHYVIFW 509
           KFVCRSNLLLSINMDKITEKDLQLAVEAAR VLAAEKLELVEFTSHADITSEPGHYVIFW
Sbjct: 426 KFVCRSNLLLSINMDKITEKDLQLAVEAARNVLAAEKLELVEFTSHADITSEPGHYVIFW 485

Query: 510 EISGEAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHYVSLG 569
           EISGEAKEEVL ECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHYVSLG
Sbjct: 486 EISGEAKEEVLRECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHYVSLG 545

Query: 570 SAMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF 605
           SAMSQYKTPRAVGSTNTAV DILCSNVV+SYFSTAF
Sbjct: 546 SAMSQYKTPRAVGSTNTAVLDILCSNVVNSYFSTAF 581

BLAST of Csor.00g180380 vs. ExPASy TrEMBL
Match: A0A6J1E9G0 (jasmonic acid-amido synthetase JAR1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111432047 PE=3 SV=1)

HSP 1 Score: 1156 bits (2990), Expect = 0.0
Identity = 576/577 (99.83%), Postives = 577/577 (100.00%), Query Frame = 0

Query: 29  RRMEVFDGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGRTDPQSFKEC 88
           +RMEVFDGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGRTDPQSFKEC
Sbjct: 3   QRMEVFDGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGRTDPQSFKEC 62

Query: 89  IPLATHDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETTMQI 148
           IPLATHDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETTMQI
Sbjct: 63  IPLATHDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETTMQI 122

Query: 149 FQTSFAFRNKQIPLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQSQ 208
           FQTSFAFRNKQIPLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQSQ
Sbjct: 123 FQTSFAFRNKQIPLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQSQ 182

Query: 209 SCSPDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELCTNI 268
           SCSPDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELCTNI
Sbjct: 183 SCSPDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELCTNI 242

Query: 269 RDGVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIYGIM 328
           RDGVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIYGIM
Sbjct: 243 RDGVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIYGIM 302

Query: 329 TGSMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEFIP 388
           TGSMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEFIP
Sbjct: 303 TGSMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEFIP 362

Query: 389 LKANAQDQEQEGAQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNATPE 448
           LKANAQDQEQEGAQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNATPE
Sbjct: 363 LKANAQDQEQEGAQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNATPE 422

Query: 449 LKFVCRSNLLLSINMDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPGHYVIF 508
           LKFVCRSNLLLSINMDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPGHYVIF
Sbjct: 423 LKFVCRSNLLLSINMDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPGHYVIF 482

Query: 509 WEISGEAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHYVSL 568
           WEISGEAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHYVSL
Sbjct: 483 WEISGEAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHYVSL 542

Query: 569 GSAMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF 605
           GSAMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF
Sbjct: 543 GSAMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF 579

BLAST of Csor.00g180380 vs. ExPASy TrEMBL
Match: A0A6J1E9N9 (jasmonic acid-amido synthetase JAR1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111432047 PE=3 SV=1)

HSP 1 Score: 1154 bits (2984), Expect = 0.0
Identity = 575/575 (100.00%), Postives = 575/575 (100.00%), Query Frame = 0

Query: 31  MEVFDGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGRTDPQSFKECIP 90
           MEVFDGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGRTDPQSFKECIP
Sbjct: 1   MEVFDGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGRTDPQSFKECIP 60

Query: 91  LATHDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETTMQIFQ 150
           LATHDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETTMQIFQ
Sbjct: 61  LATHDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETTMQIFQ 120

Query: 151 TSFAFRNKQIPLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQSQSC 210
           TSFAFRNKQIPLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQSQSC
Sbjct: 121 TSFAFRNKQIPLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQSQSC 180

Query: 211 SPDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELCTNIRD 270
           SPDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELCTNIRD
Sbjct: 181 SPDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELCTNIRD 240

Query: 271 GVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIYGIMTG 330
           GVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIYGIMTG
Sbjct: 241 GVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIYGIMTG 300

Query: 331 SMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEFIPLK 390
           SMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEFIPLK
Sbjct: 301 SMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEFIPLK 360

Query: 391 ANAQDQEQEGAQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNATPELK 450
           ANAQDQEQEGAQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNATPELK
Sbjct: 361 ANAQDQEQEGAQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNATPELK 420

Query: 451 FVCRSNLLLSINMDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPGHYVIFWE 510
           FVCRSNLLLSINMDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPGHYVIFWE
Sbjct: 421 FVCRSNLLLSINMDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPGHYVIFWE 480

Query: 511 ISGEAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHYVSLGS 570
           ISGEAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHYVSLGS
Sbjct: 481 ISGEAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHYVSLGS 540

Query: 571 AMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF 605
           AMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF
Sbjct: 541 AMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF 575

BLAST of Csor.00g180380 vs. ExPASy TrEMBL
Match: A0A6J1IP89 (jasmonic acid-amido synthetase JAR1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479304 PE=3 SV=1)

HSP 1 Score: 1106 bits (2860), Expect = 0.0
Identity = 554/582 (95.19%), Postives = 565/582 (97.08%), Query Frame = 0

Query: 29  RRMEVFDGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGRTDPQSFKEC 88
           +RMEVFDGEKVI+EFEEMTKDA RVQ+ETLKKILEENGWAEYLQNLGLNGRTDPQSFKEC
Sbjct: 3   QRMEVFDGEKVIKEFEEMTKDAERVQMETLKKILEENGWAEYLQNLGLNGRTDPQSFKEC 62

Query: 89  IPLATHDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETTMQI 148
           +PLATHDDFEAYIQ+IADGDSSPILTGKPIESMSLSSGT+KGRPKFIPFNDSLFETTMQI
Sbjct: 63  VPLATHDDFEAYIQKIADGDSSPILTGKPIESMSLSSGTTKGRPKFIPFNDSLFETTMQI 122

Query: 149 FQTSFAFRNKQIPLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQSQ 208
           FQTSFAFRNKQ+PLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQSQ
Sbjct: 123 FQTSFAFRNKQVPLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQSQ 182

Query: 209 SCSPDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELCTNI 268
           SCSPDEVIFGSDFHQSLYCHLLCGLISRDEIESV S FAHSIILPFKTFEQVWEELCTNI
Sbjct: 183 SCSPDEVIFGSDFHQSLYCHLLCGLISRDEIESVSSPFAHSIILPFKTFEQVWEELCTNI 242

Query: 269 RDGVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIYGIM 328
           RDGVLSSWVTDPSIRAAMSKLLKPDPELADLIY+KCAGL NWYGLIPELFPNAKYIYGIM
Sbjct: 243 RDGVLSSWVTDPSIRAAMSKLLKPDPELADLIYEKCAGLSNWYGLIPELFPNAKYIYGIM 302

Query: 329 TGSMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEFIP 388
           TGSMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEFIP
Sbjct: 303 TGSMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEFIP 362

Query: 389 LKANAQDQEQEG-----AQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFH 448
           LKANAQDQEQEG      QENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFH
Sbjct: 363 LKANAQDQEQEGDGALVLQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFH 422

Query: 449 NATPELKFVCRSNLLLSINMDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPG 508
           NATPELKFVCRSNLLLSIN DKITEKDLQLAVEAAR VLAAEKLELVEFTSHADITSEPG
Sbjct: 423 NATPELKFVCRSNLLLSINTDKITEKDLQLAVEAARNVLAAEKLELVEFTSHADITSEPG 482

Query: 509 HYVIFWEISGEAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIME 568
           HYVIF EISGEAKEEVL ECCNRLDKVF+EAGSVSWRKGKATGALELRVVRKGTFHKIME
Sbjct: 483 HYVIFLEISGEAKEEVLRECCNRLDKVFREAGSVSWRKGKATGALELRVVRKGTFHKIME 542

Query: 569 HYVSLGSAMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF 605
           H +SLGSAMSQYKTPRAVGSTNTAV  ILCSNVV+SYFSTAF
Sbjct: 543 HSLSLGSAMSQYKTPRAVGSTNTAVLHILCSNVVNSYFSTAF 584

BLAST of Csor.00g180380 vs. ExPASy TrEMBL
Match: A0A6J1IX50 (jasmonic acid-amido synthetase JAR1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111479304 PE=3 SV=1)

HSP 1 Score: 1103 bits (2854), Expect = 0.0
Identity = 553/580 (95.34%), Postives = 563/580 (97.07%), Query Frame = 0

Query: 31  MEVFDGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGRTDPQSFKECIP 90
           MEVFDGEKVI+EFEEMTKDA RVQ+ETLKKILEENGWAEYLQNLGLNGRTDPQSFKEC+P
Sbjct: 1   MEVFDGEKVIKEFEEMTKDAERVQMETLKKILEENGWAEYLQNLGLNGRTDPQSFKECVP 60

Query: 91  LATHDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETTMQIFQ 150
           LATHDDFEAYIQ+IADGDSSPILTGKPIESMSLSSGT+KGRPKFIPFNDSLFETTMQIFQ
Sbjct: 61  LATHDDFEAYIQKIADGDSSPILTGKPIESMSLSSGTTKGRPKFIPFNDSLFETTMQIFQ 120

Query: 151 TSFAFRNKQIPLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQSQSC 210
           TSFAFRNKQ+PLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQSQSC
Sbjct: 121 TSFAFRNKQVPLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQSQSC 180

Query: 211 SPDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELCTNIRD 270
           SPDEVIFGSDFHQSLYCHLLCGLISRDEIESV S FAHSIILPFKTFEQVWEELCTNIRD
Sbjct: 181 SPDEVIFGSDFHQSLYCHLLCGLISRDEIESVSSPFAHSIILPFKTFEQVWEELCTNIRD 240

Query: 271 GVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIYGIMTG 330
           GVLSSWVTDPSIRAAMSKLLKPDPELADLIY+KCAGL NWYGLIPELFPNAKYIYGIMTG
Sbjct: 241 GVLSSWVTDPSIRAAMSKLLKPDPELADLIYEKCAGLSNWYGLIPELFPNAKYIYGIMTG 300

Query: 331 SMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEFIPLK 390
           SMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEFIPLK
Sbjct: 301 SMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEFIPLK 360

Query: 391 ANAQDQEQEG-----AQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNA 450
           ANAQDQEQEG      QENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNA
Sbjct: 361 ANAQDQEQEGDGALVLQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNA 420

Query: 451 TPELKFVCRSNLLLSINMDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPGHY 510
           TPELKFVCRSNLLLSIN DKITEKDLQLAVEAAR VLAAEKLELVEFTSHADITSEPGHY
Sbjct: 421 TPELKFVCRSNLLLSINTDKITEKDLQLAVEAARNVLAAEKLELVEFTSHADITSEPGHY 480

Query: 511 VIFWEISGEAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHY 570
           VIF EISGEAKEEVL ECCNRLDKVF+EAGSVSWRKGKATGALELRVVRKGTFHKIMEH 
Sbjct: 481 VIFLEISGEAKEEVLRECCNRLDKVFREAGSVSWRKGKATGALELRVVRKGTFHKIMEHS 540

Query: 571 VSLGSAMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF 605
           +SLGSAMSQYKTPRAVGSTNTAV  ILCSNVV+SYFSTAF
Sbjct: 541 LSLGSAMSQYKTPRAVGSTNTAVLHILCSNVVNSYFSTAF 580

BLAST of Csor.00g180380 vs. ExPASy TrEMBL
Match: A0A6J1EFH9 (jasmonic acid-amido synthetase JAR1-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111432047 PE=3 SV=1)

HSP 1 Score: 1071 bits (2769), Expect = 0.0
Identity = 541/577 (93.76%), Postives = 543/577 (94.11%), Query Frame = 0

Query: 29  RRMEVFDGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGRTDPQSFKEC 88
           +RMEVFDGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGRTDPQSFKEC
Sbjct: 3   QRMEVFDGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGRTDPQSFKEC 62

Query: 89  IPLATHDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETTMQI 148
           IPLATHDDFEAYIQRIADGDSSPILTGKPIESMSL                         
Sbjct: 63  IPLATHDDFEAYIQRIADGDSSPILTGKPIESMSL------------------------- 122

Query: 149 FQTSFAFRNKQIPLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQSQ 208
                    +QIPLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQSQ
Sbjct: 123 ---------RQIPLGNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQSQ 182

Query: 209 SCSPDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELCTNI 268
           SCSPDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELCTNI
Sbjct: 183 SCSPDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELCTNI 242

Query: 269 RDGVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIYGIM 328
           RDGVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIYGIM
Sbjct: 243 RDGVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIYGIM 302

Query: 329 TGSMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEFIP 388
           TGSMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEFIP
Sbjct: 303 TGSMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEFIP 362

Query: 389 LKANAQDQEQEGAQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNATPE 448
           LKANAQDQEQEGAQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNATPE
Sbjct: 363 LKANAQDQEQEGAQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNATPE 422

Query: 449 LKFVCRSNLLLSINMDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPGHYVIF 508
           LKFVCRSNLLLSINMDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPGHYVIF
Sbjct: 423 LKFVCRSNLLLSINMDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPGHYVIF 482

Query: 509 WEISGEAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHYVSL 568
           WEISGEAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHYVSL
Sbjct: 483 WEISGEAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHYVSL 542

Query: 569 GSAMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF 605
           GSAMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF
Sbjct: 543 GSAMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF 545

BLAST of Csor.00g180380 vs. TAIR 10
Match: AT2G46370.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 758.8 bits (1958), Expect = 3.3e-219
Identity = 367/579 (63.39%), Postives = 459/579 (79.27%), Query Frame = 0

Query: 30  RMEVFDGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGR-TDP-QSFKE 89
           ++E FD  +VI+EF+EMT++A +VQ +TLK+IL +N  A YLQN GLNG  TDP ++FK 
Sbjct: 4   KVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATDPEEAFKS 63

Query: 90  CIPLATHDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETTMQ 149
            +PL T  + E YI+R+ DGD+SPILTG P+ ++SLSSGTS+GRPKFIPF D L E T+Q
Sbjct: 64  MVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQ 123

Query: 150 IFQTSFAFRNKQIPL-GNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQ 209
           +F+T+FAFRN+  P+  NGKALQF++SSKQ+ + GG+  G ATTNVYRNP FK  MK+I 
Sbjct: 124 LFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSIT 183

Query: 210 SQSCSPDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELCT 269
           S SCSPDEVIF  D HQ+LYCHLL G++ RD+++ VF+ FAH ++  F+TFEQVWEE+ T
Sbjct: 184 SPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIVT 243

Query: 270 NIRDGVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIYG 329
           +I+DGVLS+ +T PS+R AMSKLL P+PELA+ I  KC  L NWYGLIP LFPNAKY+YG
Sbjct: 244 DIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPNAKYVYG 303

Query: 330 IMTGSMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEF 389
           IMTGSMEPY+ KLRHYAG LPL+S DYG+SEGWI  NV P L PE ATFAVIPN+GYFEF
Sbjct: 304 IMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGYFEF 363

Query: 390 IPLKANAQDQEQEGAQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNAT 449
           +P+        + G  E KPVGLT+VKIGEEYE+V+TN AGLYRYRLGDVVKV+GF+N T
Sbjct: 364 LPV-------SETGEGEEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKVIGFYNNT 423

Query: 450 PELKFVCRSNLLLSINMDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPGHYV 509
           P+LKF+CR NL+LSIN+DK TE+DLQL+VE+A   L+ EK+E+++F+S+ D++++PGHY 
Sbjct: 424 PQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEKIEVIDFSSYIDVSTDPGHYA 483

Query: 510 IFWEISGEAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHYV 569
           IFWEISGE  E+VL +CCN LD+ F +AG VS RK K  GALELRVV KGTF KI EH++
Sbjct: 484 IFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTFRKIQEHFL 543

Query: 570 SLGSAMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF 606
            LGS+  Q+K PR V  +N  V  ILC NVVSSYFSTAF
Sbjct: 544 GLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

BLAST of Csor.00g180380 vs. TAIR 10
Match: AT2G46370.2 (Auxin-responsive GH3 family protein )

HSP 1 Score: 758.8 bits (1958), Expect = 3.3e-219
Identity = 367/579 (63.39%), Postives = 459/579 (79.27%), Query Frame = 0

Query: 30  RMEVFDGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGR-TDP-QSFKE 89
           ++E FD  +VI+EF+EMT++A +VQ +TLK+IL +N  A YLQN GLNG  TDP ++FK 
Sbjct: 4   KVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATDPEEAFKS 63

Query: 90  CIPLATHDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETTMQ 149
            +PL T  + E YI+R+ DGD+SPILTG P+ ++SLSSGTS+GRPKFIPF D L E T+Q
Sbjct: 64  MVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQ 123

Query: 150 IFQTSFAFRNKQIPL-GNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQ 209
           +F+T+FAFRN+  P+  NGKALQF++SSKQ+ + GG+  G ATTNVYRNP FK  MK+I 
Sbjct: 124 LFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSIT 183

Query: 210 SQSCSPDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELCT 269
           S SCSPDEVIF  D HQ+LYCHLL G++ RD+++ VF+ FAH ++  F+TFEQVWEE+ T
Sbjct: 184 SPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIVT 243

Query: 270 NIRDGVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIYG 329
           +I+DGVLS+ +T PS+R AMSKLL P+PELA+ I  KC  L NWYGLIP LFPNAKY+YG
Sbjct: 244 DIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPNAKYVYG 303

Query: 330 IMTGSMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEF 389
           IMTGSMEPY+ KLRHYAG LPL+S DYG+SEGWI  NV P L PE ATFAVIPN+GYFEF
Sbjct: 304 IMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGYFEF 363

Query: 390 IPLKANAQDQEQEGAQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNAT 449
           +P+        + G  E KPVGLT+VKIGEEYE+V+TN AGLYRYRLGDVVKV+GF+N T
Sbjct: 364 LPV-------SETGEGEEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKVIGFYNNT 423

Query: 450 PELKFVCRSNLLLSINMDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPGHYV 509
           P+LKF+CR NL+LSIN+DK TE+DLQL+VE+A   L+ EK+E+++F+S+ D++++PGHY 
Sbjct: 424 PQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEKIEVIDFSSYIDVSTDPGHYA 483

Query: 510 IFWEISGEAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHYV 569
           IFWEISGE  E+VL +CCN LD+ F +AG VS RK K  GALELRVV KGTF KI EH++
Sbjct: 484 IFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTFRKIQEHFL 543

Query: 570 SLGSAMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF 606
            LGS+  Q+K PR V  +N  V  ILC NVVSSYFSTAF
Sbjct: 544 GLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

BLAST of Csor.00g180380 vs. TAIR 10
Match: AT2G46370.4 (Auxin-responsive GH3 family protein )

HSP 1 Score: 758.8 bits (1958), Expect = 3.3e-219
Identity = 367/579 (63.39%), Postives = 459/579 (79.27%), Query Frame = 0

Query: 30  RMEVFDGEKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQNLGLNGR-TDP-QSFKE 89
           ++E FD  +VI+EF+EMT++A +VQ +TLK+IL +N  A YLQN GLNG  TDP ++FK 
Sbjct: 15  KVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATDPEEAFKS 74

Query: 90  CIPLATHDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETTMQ 149
            +PL T  + E YI+R+ DGD+SPILTG P+ ++SLSSGTS+GRPKFIPF D L E T+Q
Sbjct: 75  MVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQ 134

Query: 150 IFQTSFAFRNKQIPL-GNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQ 209
           +F+T+FAFRN+  P+  NGKALQF++SSKQ+ + GG+  G ATTNVYRNP FK  MK+I 
Sbjct: 135 LFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSIT 194

Query: 210 SQSCSPDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELCT 269
           S SCSPDEVIF  D HQ+LYCHLL G++ RD+++ VF+ FAH ++  F+TFEQVWEE+ T
Sbjct: 195 SPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIVT 254

Query: 270 NIRDGVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIYG 329
           +I+DGVLS+ +T PS+R AMSKLL P+PELA+ I  KC  L NWYGLIP LFPNAKY+YG
Sbjct: 255 DIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPNAKYVYG 314

Query: 330 IMTGSMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEF 389
           IMTGSMEPY+ KLRHYAG LPL+S DYG+SEGWI  NV P L PE ATFAVIPN+GYFEF
Sbjct: 315 IMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGYFEF 374

Query: 390 IPLKANAQDQEQEGAQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNAT 449
           +P+        + G  E KPVGLT+VKIGEEYE+V+TN AGLYRYRLGDVVKV+GF+N T
Sbjct: 375 LPV-------SETGEGEEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKVIGFYNNT 434

Query: 450 PELKFVCRSNLLLSINMDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPGHYV 509
           P+LKF+CR NL+LSIN+DK TE+DLQL+VE+A   L+ EK+E+++F+S+ D++++PGHY 
Sbjct: 435 PQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEKIEVIDFSSYIDVSTDPGHYA 494

Query: 510 IFWEISGEAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHYV 569
           IFWEISGE  E+VL +CCN LD+ F +AG VS RK K  GALELRVV KGTF KI EH++
Sbjct: 495 IFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTFRKIQEHFL 554

Query: 570 SLGSAMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF 606
            LGS+  Q+K PR V  +N  V  ILC NVVSSYFSTAF
Sbjct: 555 GLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 586

BLAST of Csor.00g180380 vs. TAIR 10
Match: AT2G46370.3 (Auxin-responsive GH3 family protein )

HSP 1 Score: 686.8 bits (1771), Expect = 1.6e-197
Identity = 327/503 (65.01%), Postives = 403/503 (80.12%), Query Frame = 0

Query: 104 IADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETTMQIFQTSFAFRNKQIPL- 163
           + DGD+SPILTG P+ ++SLSSGTS+GRPKFIPF D L E T+Q+F+T+FAFRN+  P+ 
Sbjct: 1   MVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQLFRTAFAFRNRDFPID 60

Query: 164 GNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQSQSCSPDEVIFGSDFH 223
            NGKALQF++SSKQ+ + GG+  G ATTNVYRNP FK  MK+I S SCSPDEVIF  D H
Sbjct: 61  DNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSITSPSCSPDEVIFSPDVH 120

Query: 224 QSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELCTNIRDGVLSSWVTDPSI 283
           Q+LYCHLL G++ RD+++ VF+ FAH ++  F+TFEQVWEE+ T+I+DGVLS+ +T PS+
Sbjct: 121 QALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIVTDIKDGVLSNRITVPSV 180

Query: 284 RAAMSKLLKPDPELADLIYKKCAGLINWYGLIPELFPNAKYIYGIMTGSMEPYLMKLRHY 343
           R AMSKLL P+PELA+ I  KC  L NWYGLIP LFPNAKY+YGIMTGSMEPY+ KLRHY
Sbjct: 181 RTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPNAKYVYGIMTGSMEPYVPKLRHY 240

Query: 344 AGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFEFIPLKANAQDQEQEGAQ 403
           AG LPL+S DYG+SEGWI  NV P L PE ATFAVIPN+GYFEF+P+        + G  
Sbjct: 241 AGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGYFEFLPV-------SETGEG 300

Query: 404 ENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGFHNATPELKFVCRSNLLLSIN 463
           E KPVGLT+VKIGEEYE+V+TN AGLYRYRLGDVVKV+GF+N TP+LKF+CR NL+LSIN
Sbjct: 301 EEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKVIGFYNNTPQLKFICRRNLILSIN 360

Query: 464 MDKITEKDLQLAVEAARTVLAAEKLELVEFTSHADITSEPGHYVIFWEISGEAKEEVLIE 523
           +DK TE+DLQL+VE+A   L+ EK+E+++F+S+ D++++PGHY IFWEISGE  E+VL +
Sbjct: 361 IDKNTERDLQLSVESAAKRLSEEKIEVIDFSSYIDVSTDPGHYAIFWEISGETNEDVLQD 420

Query: 524 CCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKIMEHYVSLGSAMSQYKTPRAVG 583
           CCN LD+ F +AG VS RK K  GALELRVV KGTF KI EH++ LGS+  Q+K PR V 
Sbjct: 421 CCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTFRKIQEHFLGLGSSAGQFKMPRCVK 480

Query: 584 STNTAVFDILCSNVVSSYFSTAF 606
            +N  V  ILC NVVSSYFSTAF
Sbjct: 481 PSNAKVLQILCENVVSSYFSTAF 496

BLAST of Csor.00g180380 vs. TAIR 10
Match: AT4G03400.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 552.0 bits (1421), Expect = 6.0e-157
Identity = 285/584 (48.80%), Postives = 401/584 (68.66%), Query Frame = 0

Query: 37  EKVIEEFEEMTKDAGRVQVETLKKILEENGWAEYLQN-LG------LNGRTDPQSFKECI 96
           + VI  FE ++++A +VQ ETL++ILE N   EYL+  LG      ++  T    F   +
Sbjct: 9   DDVIGWFEHVSENACKVQSETLRRILELNSGVEYLRKWLGTVDVEKMDDYTLETLFTSLV 68

Query: 97  PLATHDDFEAYIQRIADGDSSPILTGKPIESMSLSSGTSKGRPKFIPFNDSLFETTMQIF 156
           P+ +H D + YIQRIADG++SP+LT +PI  +SLSSGT++GR K++PF     +TT+QIF
Sbjct: 69  PIVSHADLDPYIQRIADGETSPLLTQEPITVLSLSSGTTEGRQKYVPFTRHSAQTTLQIF 128

Query: 157 QTSFAFRNKQIPL-GNGKALQFVYSSKQFKTKGGLAAGAATTNVYRNPKFKTTMKAIQSQ 216
           + S A+R++  P+   G+ L+F+Y+ K+FKT GGL  G ATT+ Y + +FKT  +  +S 
Sbjct: 129 RLSAAYRSRFYPIREGGRILEFIYAGKEFKTLGGLTVGTATTHYYASEEFKTKQETTKSF 188

Query: 217 SCSPDEVIFGSDFHQSLYCHLLCGLISRDEIESVFSSFAHSIILPFKTFEQVWEELCTNI 276
           +CSP EVI G DF Q  YCHLL GL    ++E V S+F+++I+  F  FE++W E+C +I
Sbjct: 189 TCSPQEVISGGDFGQCTYCHLLLGLHYSSQVEFVASAFSYTIVQAFSFFEEIWREICADI 248

Query: 277 RDGVLSSWVTDPSIRAAMSKLLKPDPELADLIYKKCAGL---INWYGLIPELFPNAKYIY 336
           ++G LSS +T P +R A+  L++P+P LA  I + C  L   + W+GLI +L+PNAK+I 
Sbjct: 249 KEGNLSSRITLPKMRKAVLALIRPNPSLASHIEEICLELETNLGWFGLISKLWPNAKFIS 308

Query: 337 GIMTGSMEPYLMKLRHYAGHLPLISADYGASEGWIGVNVNPMLPPEMATFAVIPNIGYFE 396
            IMTGSM PYL KLRHYAG LPL+SADYG++E WIGVNV+P LPPE  +FAVIP   YFE
Sbjct: 309 SIMTGSMLPYLNKLRHYAGGLPLVSADYGSTESWIGVNVDPHLPPEDVSFAVIPTFSYFE 368

Query: 397 FIPL--KANAQDQEQEG-AQENKPVGLTEVKIGEEYEIVVTNVAGLYRYRLGDVVKVMGF 456
           FIPL  + N  D   +G   E+KPV L++VK+G+EYE+V+T   GLYRYRLGDVV+V  F
Sbjct: 369 FIPLYRRQNQSDICIDGDFVEDKPVPLSQVKLGQEYELVLTTFTGLYRYRLGDVVEVTSF 428

Query: 457 HNATPELKFVCRSNLLLSINMDKITEKDLQLAVEAARTVLA-AEKLELVEFTSHADITSE 516
           H  TP+L F+ R  L+L+IN+DK TEKDLQ  V+ A  +L+ + + E+V+FTSHAD+ + 
Sbjct: 429 HKGTPKLSFIYRRKLILTINIDKNTEKDLQRVVDKASQLLSRSTRAEVVDFTSHADVIAR 488

Query: 517 PGHYVIFWEISGEAKEEVLIECCNRLDKVFQEAGSVSWRKGKATGALELRVVRKGTFHKI 576
           PGHYVI+WEI GEA ++ L ECC  +D  F + G V  R+  + G LELRVV +GTF K+
Sbjct: 489 PGHYVIYWEIRGEADDKALEECCREMDTAFVDYGYVVSRRMNSIGPLELRVVERGTFGKV 548

Query: 577 MEHYVSLGSAMSQYKTPRAVGSTNTAVFDILCSNVVSSYFSTAF 606
            E  V     ++Q+KTPR   +TN+ + DIL  + +  + S+A+
Sbjct: 549 AERCVGKCGGLNQFKTPRC--TTNSVMLDILNDSTIKRFRSSAY 590

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A314KSQ42.4e-25173.08Jasmonoyl--L-amino acid synthetase JAR6 OS=Nicotiana attenuata OX=49451 GN=JAR6 ... [more]
A0A1J6KGJ97.2e-24871.21Jasmonoyl--L-amino acid synthetase JAR4 OS=Nicotiana attenuata OX=49451 GN=JAR4 ... [more]
Q6I5814.6e-22664.59Jasmonoyl--L-amino acid synthetase GH3.5 OS=Oryza sativa subsp. japonica OX=3994... [more]
Q9SKE24.6e-21863.39Jasmonoyl--L-amino acid synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 ... [more]
Q53P492.6e-16548.49Probable indole-3-acetic acid-amido synthetase GH3.12 OS=Oryza sativa subsp. jap... [more]
Match NameE-valueIdentityDescription
KAG6582660.10.0100.00Jasmonoyl--L-amino acid synthetase JAR6, partial [Cucurbita argyrosperma subsp. ... [more]
KAG7019058.10.099.48Jasmonic acid-amido synthetase JAR1, partial [Cucurbita argyrosperma subsp. argy... [more]
XP_022924607.10.099.83jasmonic acid-amido synthetase JAR1-like isoform X1 [Cucurbita moschata] >XP_022... [more]
XP_022924609.10.0100.00jasmonic acid-amido synthetase JAR1-like isoform X2 [Cucurbita moschata][more]
XP_023527408.10.097.22jasmonic acid-amido synthetase JAR1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1E9G00.099.83jasmonic acid-amido synthetase JAR1-like isoform X1 OS=Cucurbita moschata OX=366... [more]
A0A6J1E9N90.0100.00jasmonic acid-amido synthetase JAR1-like isoform X2 OS=Cucurbita moschata OX=366... [more]
A0A6J1IP890.095.19jasmonic acid-amido synthetase JAR1-like isoform X1 OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1IX500.095.34jasmonic acid-amido synthetase JAR1-like isoform X2 OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1EFH90.093.76jasmonic acid-amido synthetase JAR1-like isoform X3 OS=Cucurbita moschata OX=366... [more]
Match NameE-valueIdentityDescription
AT2G46370.13.3e-21963.39Auxin-responsive GH3 family protein [more]
AT2G46370.23.3e-21963.39Auxin-responsive GH3 family protein [more]
AT2G46370.43.3e-21963.39Auxin-responsive GH3 family protein [more]
AT2G46370.31.6e-19765.01Auxin-responsive GH3 family protein [more]
AT4G03400.16.0e-15748.80Auxin-responsive GH3 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004993GH3 familyPFAMPF03321GH3coord: 42..583
e-value: 5.4E-183
score: 609.6
IPR004993GH3 familyPANTHERPTHR31901GH3 DOMAIN-CONTAINING PROTEINcoord: 32..591
NoneNo IPR availablePANTHERPTHR31901:SF40JASMONIC ACID-AMIDO SYNTHETASE JAR1-LIKE ISOFORM X1coord: 32..591

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g180380.m01Csor.00g180380.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0102053 (-)-jasmonoyl-isoleucine synthetase activity
molecular_function GO:0102058 jasmonoyl-leucine synthetase activity
molecular_function GO:0102057 jasmonoyl-valine synthetase activity