Csor.00g173480 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g173480
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptioncytochrome P450 84A1-like
LocationCsor_Chr11: 6199758 .. 6201332 (+)
RNA-Seq ExpressionCsor.00g173480
SyntenyCsor.00g173480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintronterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTCTTTGAATTCTTCCTCCTTCTTCTTCTTCTTCCTCGTTTCTCTTCTGTTCTTCCTCGCTTTCCTATGGAGATTACGCCGTAAACTCCCTTATCCGCCCGGCCCTACCGGCCTCCCTATCATCGGCAACATGCTCATGATGAACGAACTAACTCACCGTGGCCTCACTCGTCTCGCCGCCATCTACGGCGGCCTTTTCCACCTCCGTCTCGGCGTTCTCCATATGGTTGTCGTTTCCACGCCAGAAATGGCACGCGAGTTCCTCCAAGTTCAGGATATCGCATTCGCAAATAGACCTGCAAACGTCGCGATATCGTACCTCACGTACGACCGCGCGGATATGGCCTTCGCAAATTACGGTCCATTCTGGCGCCAAATGCGCAAGATCTGTGTCATGAAGCTTTTCAGTCGACGGCGGGCTGAATCATGGGCGTCAGTGCGCGATGAAGTCGATTCTGTACTTCAAATCGTATCGAATAAAATCGGCGAGCCTGTGAATATAGGCGAACTCGTTTTCGGTCTGACACGAAACATAACCTACAAGGCCGCGTTTGGATCGTGCTCGCACGAAGGACAAGACGAATTCGTGGAAATCCTGCAAGAATTTTCTAAACTGTTTGGCGCTTTTAACATTGCAGATTTTTTGCCATGGTTAGGCTGGATTCATGCTGGAGAATTCAATCGAAGAATGGAAAAAGCTAGAAAATCTCTTGATGTGTTCATTGATAGCATAATCGATGAACATATAGCAAAAAAATTGGGGAAAAACGATGATAATGATGAACAGGACGTTGATTCCGATATGGTGGATGAATTAATGGCGTTCCTAGTCGATGAATCGAGTAGCAATGATTTTCAACTTACTAGAGATAATATCAAAGCGATAATCATGGTGAGTTCATCGATTTTTCTATTGATTTGCAAAACTGATTTGATTCATAATGTTCATGAGATTTTTGCAGGATGTGATGTTCGGCGGAACAGAGACGGTTGCTTCTGTGATTGAATGGTCCATGGCGGAATTAATGAGAAATCCGGAAGAGTTACAAAAGCTTCAAGAAGAACTCACACGGACTGTTGGATTAGATCGAAGACTCCATGAATCGGACCTTCAGAATCTGCCCTACCTGAAAGCCGTCGTGAAGGAGACTCTCCGCCTTCATCCGCCGATTCCGTTGCTCCTCCACGAGACCGCCGTGGACACCGCCGTTGCAGGCTACTTCATTCCGACTGGTTCAAGAGTGTGGATCAACTGCTGGGCTATTGGCCGGGATGGAACCGCTTGGGAAGATCCGGACCGGTTTCGACCAGGGAGATTTGAAAACGACGCCGCTCCGGATTTCAAAGGGAATGATTTTGAGTTCCTTCCGTTCGGGTCGGGTCGGCGGTCTTGCCCCGGAATGCAACTCGGACTTTATGCTTGTGAGATGGCGGTGGCTAATCTTCTTCATTCCTTCTCGTGGGAGTTGCCGGACGGAATGAAGGCGGAGGAGCTGGACATGAACGACTCGTTTGGTTTAACCGCTCCCAGAGCGGTTCGACTCGTTGCTGTGCCGAGTTACCGGCTTAA

mRNA sequence

ATGGAGTCTTTGAATTCTTCCTCCTTCTTCTTCTTCTTCCTCGTTTCTCTTCTGTTCTTCCTCGCTTTCCTATGGAGATTACGCCGTAAACTCCCTTATCCGCCCGGCCCTACCGGCCTCCCTATCATCGGCAACATGCTCATGATGAACGAACTAACTCACCGTGGCCTCACTCGTCTCGCCGCCATCTACGGCGGCCTTTTCCACCTCCGTCTCGGCGTTCTCCATATGGTTGTCGTTTCCACGCCAGAAATGGCACGCGAGTTCCTCCAAGTTCAGGATATCGCATTCGCAAATAGACCTGCAAACGTCGCGATATCGTACCTCACGTACGACCGCGCGGATATGGCCTTCGCAAATTACGGTCCATTCTGGCGCCAAATGCGCAAGATCTGTGTCATGAAGCTTTTCAGTCGACGGCGGGCTGAATCATGGGCGTCAGTGCGCGATGAAGTCGATTCTGTACTTCAAATCGTATCGAATAAAATCGGCGAGCCTGTGAATATAGGCGAACTCGTTTTCGGTCTGACACGAAACATAACCTACAAGGCCGCGTTTGGATCGTGCTCGCACGAAGGACAAGACGAATTCGTGGAAATCCTGCAAGAATTTTCTAAACTGTTTGGCGCTTTTAACATTGCAGATTTTTTGCCATGGTTAGGCTGGATTCATGCTGGAGAATTCAATCGAAGAATGGAAAAAGCTAGAAAATCTCTTGATGTGTTCATTGATAGCATAATCGATGAACATATAGCAAAAAAATTGGGGAAAAACGATGATAATGATGAACAGGACGTTGATTCCGATATGGTGGATGAATTAATGGCGTTCCTAGTCGATGAATCGAGTAGCAATGATTTTCAACTTACTAGAGATAATATCAAAGCGATAATCATGAGCGGTTCGACTCGTTGCTGTGCCGAGTTACCGGCTTAA

Coding sequence (CDS)

ATGGAGTCTTTGAATTCTTCCTCCTTCTTCTTCTTCTTCCTCGTTTCTCTTCTGTTCTTCCTCGCTTTCCTATGGAGATTACGCCGTAAACTCCCTTATCCGCCCGGCCCTACCGGCCTCCCTATCATCGGCAACATGCTCATGATGAACGAACTAACTCACCGTGGCCTCACTCGTCTCGCCGCCATCTACGGCGGCCTTTTCCACCTCCGTCTCGGCGTTCTCCATATGGTTGTCGTTTCCACGCCAGAAATGGCACGCGAGTTCCTCCAAGTTCAGGATATCGCATTCGCAAATAGACCTGCAAACGTCGCGATATCGTACCTCACGTACGACCGCGCGGATATGGCCTTCGCAAATTACGGTCCATTCTGGCGCCAAATGCGCAAGATCTGTGTCATGAAGCTTTTCAGTCGACGGCGGGCTGAATCATGGGCGTCAGTGCGCGATGAAGTCGATTCTGTACTTCAAATCGTATCGAATAAAATCGGCGAGCCTGTGAATATAGGCGAACTCGTTTTCGGTCTGACACGAAACATAACCTACAAGGCCGCGTTTGGATCGTGCTCGCACGAAGGACAAGACGAATTCGTGGAAATCCTGCAAGAATTTTCTAAACTGTTTGGCGCTTTTAACATTGCAGATTTTTTGCCATGGTTAGGCTGGATTCATGCTGGAGAATTCAATCGAAGAATGGAAAAAGCTAGAAAATCTCTTGATGTGTTCATTGATAGCATAATCGATGAACATATAGCAAAAAAATTGGGGAAAAACGATGATAATGATGAACAGGACGTTGATTCCGATATGGTGGATGAATTAATGGCGTTCCTAGTCGATGAATCGAGTAGCAATGATTTTCAACTTACTAGAGATAATATCAAAGCGATAATCATGAGCGGTTCGACTCGTTGCTGTGCCGAGTTACCGGCTTAA

Protein sequence

MESLNSSSFFFFFLVSLLFFLAFLWRLRRKLPYPPGPTGLPIIGNMLMMNELTHRGLTRLAAIYGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFANYGPFWRQMRKICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGEPVNIGELVFGLTRNITYKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHAGEFNRRMEKARKSLDVFIDSIIDEHIAKKLGKNDDNDEQDVDSDMVDELMAFLVDESSSNDFQLTRDNIKAIIMSGSTRCCAELPA
Homology
BLAST of Csor.00g173480 vs. ExPASy Swiss-Prot
Match: Q42600 (Cytochrome P450 84A1 OS=Arabidopsis thaliana OX=3702 GN=CYP84A1 PE=1 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 2.1e-105
Identity = 191/307 (62.21%), Postives = 236/307 (76.87%), Query Frame = 0

Query: 1   MESLNSSSFFFFFLVSLLFFLAFLWRLRRKLPYPPGPTGLPIIGNMLMMNELTHRGLTRL 60
           +  L+  +     +VSL  F++F+ R RR+ PYPPGP G PIIGNMLMM++LTHRGL  L
Sbjct: 9   LSKLSDPTTSLVIVVSLFIFISFITR-RRRPPYPPGPRGWPIIGNMLMMDQLTHRGLANL 68

Query: 61  AAIYGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFAN 120
           A  YGGL HLR+G LHM  VS+PE+AR+ LQVQD  F+NRPA +AISYLTYDRADMAFA+
Sbjct: 69  AKKYGGLCHLRMGFLHMYAVSSPEVARQVLQVQDSVFSNRPATIAISYLTYDRADMAFAH 128

Query: 121 YGPFWRQMRKICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGEPVNIGELVFGLTRNI 180
           YGPFWRQMRK+CVMK+FSR+RAESWASVRDEVD +++ VS  +G+P+N+GE +F LTRNI
Sbjct: 129 YGPFWRQMRKVCVMKVFSRKRAESWASVRDEVDKMVRSVSCNVGKPINVGEQIFALTRNI 188

Query: 181 TYKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHAGEFNRRMEKARKSLD 240
           TY+AAFGS   +GQDEF+ ILQEFSKLFGAFN+ADF+P+ GWI     N+R+ KAR  LD
Sbjct: 189 TYRAAFGSACEKGQDEFIRILQEFSKLFGAFNVADFIPYFGWIDPQGINKRLVKARNDLD 248

Query: 241 VFIDSIIDEHIAKKLGKNDDNDEQDVDSDMVDELMAFLVDESS--------SNDFQLTRD 300
            FID IIDEH+ KK  +N  +D   VD+DMVD+L+AF  +E+          N  +LTRD
Sbjct: 249 GFIDDIIDEHMKKKENQNAVDDGDVVDTDMVDDLLAFYSEEAKLVSETADLQNSIKLTRD 308

BLAST of Csor.00g173480 vs. ExPASy Swiss-Prot
Match: F4JW83 (Cytochrome P450 84A4 OS=Arabidopsis thaliana OX=3702 GN=CYP84A4 PE=1 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 1.3e-86
Identity = 166/296 (56.08%), Postives = 220/296 (74.32%), Query Frame = 0

Query: 14  LVSLLFFLAFLWRLRRKL---PYPPGPTGLPIIGNMLMMNELTHRGLTRLAAIYGGLFHL 73
           LV LL FL     LRR++   PYPPGP GLP+IGN+LMMN+  HRGL +L+ IYGGL HL
Sbjct: 10  LVPLLLFLFPHLLLRRQMLLKPYPPGPKGLPVIGNILMMNQFNHRGLAKLSRIYGGLLHL 69

Query: 74  RLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFANYGPFWRQMRK 133
           RLG  H+ VVS+P++AR+ LQVQD  F+NRP  +AI YLTY  +D+AF NYGPFWR+MRK
Sbjct: 70  RLGFSHIFVVSSPDIARQVLQVQDHVFSNRPTTIAIRYLTYGGSDLAFCNYGPFWRRMRK 129

Query: 134 ICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGEPVNIGELVFGLTRNITYKAAFGSCS 193
           + VM LFSR+RAESW SV +EV   +++V++ +G+P+NI +L F L+R+IT++AAFGS S
Sbjct: 130 LYVMMLFSRKRAESWVSVDEEVHKSVRLVASNVGKPLNICKLAFSLSRDITFRAAFGSSS 189

Query: 194 HEGQ----DEFVEILQEFSKLFGAFNIADFLP-WLGWIHAGEFNRRMEKARKSLDVFIDS 253
                   DEF+EI+QEFSKLFG FN+AD++P WL WI     N R+EKARKSLD FI+S
Sbjct: 190 STSDESRLDEFLEIIQEFSKLFGEFNVADYVPSWLSWIDPQGINGRVEKARKSLDGFIES 249

Query: 254 IIDEHIAKKLGKNDDNDEQDVDSDMVDELMAFLVDESSSND--FQLTRDNIKAIIM 300
           +ID+H+ KK  ++D+ DE   ++DMVD+L+AF  +E   N+   ++  DNIK IIM
Sbjct: 250 VIDDHLHKKKREHDNVDE---ETDMVDQLLAFYEEEVKVNNSVTKINLDNIKGIIM 302

BLAST of Csor.00g173480 vs. ExPASy Swiss-Prot
Match: A6YIH8 (Premnaspirodiene oxygenase OS=Hyoscyamus muticus OX=35626 GN=CYP71D55 PE=1 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 5.2e-48
Identity = 118/298 (39.60%), Postives = 177/298 (59.40%), Query Frame = 0

Query: 11  FFFLVSLLFFLAFLWRLRR-------KLPYPPGPTGLPIIGNML-MMNELTHRGLTRLAA 70
           FF LVS+  FL+FL+ LR+           PPGP  LP++G+ML M+  L H  L  LA 
Sbjct: 3   FFSLVSIFLFLSFLFLLRKWKNSNSQSKKLPPGPWKLPLLGSMLHMVGGLPHHVLRDLAK 62

Query: 71  IYGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFANYG 130
            YG L HL+LG +  VVV++P+MA+E L+  DIAFA+RP  +A   + Y+R+D+AF  YG
Sbjct: 63  KYGPLMHLQLGEVSAVVVTSPDMAKEVLKTHDIAFASRPKLLAPEIVCYNRSDIAFCPYG 122

Query: 131 PFWRQMRKICVMKLFSRRRAESWASV-RDEVDSVLQIVSNKIGEPVNIGELVFGLTRNIT 190
            +WRQMRKICV+++ S +   S++S+ RDEV  ++  V +   EPVN  E +F  T ++T
Sbjct: 123 DYWRQMRKICVLEVLSAKNVRSFSSIRRDEVLRLVNFVRSSTSEPVNFTERLFLFTSSMT 182

Query: 191 YKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHA-GEFNRRMEKARKSLD 250
            ++AFG    E Q+ F+++++E   L G F++AD  P L ++H       ++ KA   +D
Sbjct: 183 CRSAFGKVFKE-QETFIQLIKEVIGLAGGFDVADIFPSLKFLHVLTGMEGKIMKAHHKVD 242

Query: 251 VFIDSIIDEHIAKKLGKNDDNDEQDVDSDMVDELMAFLVDESSSNDFQLTRDNIKAII 299
             ++ +I+EH  K L     N       D++D L+  + D      F +T DNIKAII
Sbjct: 243 AIVEDVINEH-KKNLAMGKTNGALG-GEDLIDVLLRLMND--GGLQFPITNDNIKAII 295

BLAST of Csor.00g173480 vs. ExPASy Swiss-Prot
Match: W8JCE8 (Cytochrome P450 71BT1 OS=Catharanthus roseus OX=4058 GN=CYP71BT1 PE=2 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 2.2e-46
Identity = 116/302 (38.41%), Postives = 182/302 (60.26%), Query Frame = 0

Query: 11  FFFLVSLLFFLAFLWRLRRK---LPYPPGPTGLPIIGNMLMM-NELTHRGLTRLAAIYGG 70
           F FL +LL F   L +L +K   L  PPGP  LPIIGN+  +   L H  L  +A  YG 
Sbjct: 5   FIFLFALLLFCFMLLKLSKKYSALKLPPGPRPLPIIGNLHQIGGGLMHHILREMARTYGP 64

Query: 71  LFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAI-SYLTYDRADMAFANYGPFW 130
           LF ++ G +  +VVS+PE+A+E  +  D+ F+ RP N+A+ + +TY+ +++AFA YG +W
Sbjct: 65  LFRIKFGQVSTIVVSSPEIAKEIFRTHDVVFSYRPQNIAVFNVMTYNFSNIAFAPYGNYW 124

Query: 131 RQMRKICVMKLFSRRRAESWASVR-DEVDSVLQIVSNKIGEPVNIGELVFGLTRNITYKA 190
           RQMRKICVM++    R  S+ S+R +EV ++++ V +  G+ VN+ E ++GLT +IT +A
Sbjct: 125 RQMRKICVMEILGASRVRSFGSIRQEEVMNLIRFVGSHKGKLVNVSEKIYGLTYSITARA 184

Query: 191 AFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIH-AGEFNRRMEKARKSLDVFI 250
           AFG  S + Q  F E + ++  L    +IADF P + ++       R+++   K +D  +
Sbjct: 185 AFGKVS-KYQQVFKEHMDKYDDLARGLDIADFYPSMKFLRLVTGMERKLKVMFKEVDEIL 244

Query: 251 DSIIDEH----IAKKLGKNDDN--DEQDVDSDMVDELMAFLVDESSSNDFQLTRDNIKAI 300
             IIDEH    + K   K DDN  +E++ + D+VD LM   + +S   +  +T DNIKA+
Sbjct: 245 QVIIDEHRDQRMKKIRSKVDDNEKEEEEGNEDIVDVLMN--LQQSGQTELPVTDDNIKAV 303

BLAST of Csor.00g173480 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 185.3 bits (469), Expect = 1.1e-45
Identity = 114/304 (37.50%), Postives = 171/304 (56.25%), Query Frame = 0

Query: 12  FFLVSLLFFLAFLWRLRRKLP-YPPGPTGLPIIGNMLMMNELTHRGLTRLAAIYGGLFHL 71
           F  ++L FFL  L   R K P  PP P  LPIIGN+  +  L HR L  LA   G L  L
Sbjct: 9   FLAIALTFFLLKLNEKREKKPNLPPSPPNLPIIGNLHQLGNLPHRSLRSLANELGPLILL 68

Query: 72  RLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFANYGPFWRQMRK 131
            LG +  ++VST E+A E L+  D+ FA+RP+  A   + YD  D+AF+ YG +WRQ+RK
Sbjct: 69  HLGHIPTLIVSTAEIAEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYGEYWRQVRK 128

Query: 132 ICVMKLFSRRRAESWASVR-DEVDSVLQIVSN--KIGEPVNIGELVFGLTRNITYKAAFG 191
           ICV++L S +R  S+ S+R +EV  +++ +S     GE VN+ EL+  L+     + AFG
Sbjct: 129 ICVLELLSIKRVNSYRSIREEEVGLMMERISQSCSTGEAVNLSELLLLLSSGTITRVAFG 188

Query: 192 SCSHEGQDE----FVEILQEFSKLFGAFNIADFLPWLGWIHA-GEFNRRMEKARKSLDVF 251
              +EG++E    F ++  E + L GAF + D+ P   W+      + R+++    LD F
Sbjct: 189 K-KYEGEEERKNKFADLATELTTLMGAFFVGDYFPSFAWVDVLTGMDARLKRNHGELDAF 248

Query: 252 IDSIIDEHIAKKLGKNDDNDEQDVDSDMVDELMAFLVDESSSNDFQLTRDNIKAIIM--- 304
           +D +ID+H+  +     D  EQ    D+VD L+   + + SS    L R+N+KA+I+   
Sbjct: 249 VDHVIDDHLLSRKANGSDGVEQ---KDLVDVLLH--LQKDSSLGVHLNRNNLKAVILDMF 306

BLAST of Csor.00g173480 vs. NCBI nr
Match: KAG6588421.1 (Cytochrome P450 84A1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 612 bits (1577), Expect = 1.36e-220
Identity = 311/311 (100.00%), Postives = 311/311 (100.00%), Query Frame = 0

Query: 1   MESLNSSSFFFFFLVSLLFFLAFLWRLRRKLPYPPGPTGLPIIGNMLMMNELTHRGLTRL 60
           MESLNSSSFFFFFLVSLLFFLAFLWRLRRKLPYPPGPTGLPIIGNMLMMNELTHRGLTRL
Sbjct: 1   MESLNSSSFFFFFLVSLLFFLAFLWRLRRKLPYPPGPTGLPIIGNMLMMNELTHRGLTRL 60

Query: 61  AAIYGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFAN 120
           AAIYGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFAN
Sbjct: 61  AAIYGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFAN 120

Query: 121 YGPFWRQMRKICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGEPVNIGELVFGLTRNI 180
           YGPFWRQMRKICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGEPVNIGELVFGLTRNI
Sbjct: 121 YGPFWRQMRKICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGEPVNIGELVFGLTRNI 180

Query: 181 TYKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHAGEFNRRMEKARKSLD 240
           TYKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHAGEFNRRMEKARKSLD
Sbjct: 181 TYKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHAGEFNRRMEKARKSLD 240

Query: 241 VFIDSIIDEHIAKKLGKNDDNDEQDVDSDMVDELMAFLVDESSSNDFQLTRDNIKAIIMS 300
           VFIDSIIDEHIAKKLGKNDDNDEQDVDSDMVDELMAFLVDESSSNDFQLTRDNIKAIIMS
Sbjct: 241 VFIDSIIDEHIAKKLGKNDDNDEQDVDSDMVDELMAFLVDESSSNDFQLTRDNIKAIIMS 300

Query: 301 GSTRCCAELPA 311
           GSTRCCAELPA
Sbjct: 301 GSTRCCAELPA 311

BLAST of Csor.00g173480 vs. NCBI nr
Match: KAG7022266.1 (Cytochrome P450 84A1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 586 bits (1511), Expect = 1.92e-207
Identity = 302/312 (96.79%), Postives = 303/312 (97.12%), Query Frame = 0

Query: 1   MESLNSSSFFFFFLVSLLFFLAFLWRLRRKLPYPPGPTGLPIIGNMLMMNELTHRGLTRL 60
           MESLNSSSFFFFFLVSLLFFLAFLWRLRRKLPYPPGPTGLPIIGNMLMMNELTHRGLTRL
Sbjct: 1   MESLNSSSFFFFFLVSLLFFLAFLWRLRRKLPYPPGPTGLPIIGNMLMMNELTHRGLTRL 60

Query: 61  AAIYGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFAN 120
           AAIYGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFAN
Sbjct: 61  AAIYGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFAN 120

Query: 121 YGPFWRQMRKICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGEPVNIGELVFGLTRNI 180
           YGPFWRQMRKICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGEPVNIGELVFGLTRNI
Sbjct: 121 YGPFWRQMRKICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGEPVNIGELVFGLTRNI 180

Query: 181 TYKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHAGEFNRRMEKARKSLD 240
           TYKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHAGEFNRRMEKARKSLD
Sbjct: 181 TYKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHAGEFNRRMEKARKSLD 240

Query: 241 VFIDSIIDEHIAKKLGKNDDNDEQDVDSDMVDELMAFLVDESSSNDFQLTRDNIKAIIMS 300
           VFIDSIIDEHIAKKLGKNDDNDEQDVDSDMVDELMAFLVDESSSNDFQLTRDNIKAIIM 
Sbjct: 241 VFIDSIIDEHIAKKLGKNDDNDEQDVDSDMVDELMAFLVDESSSNDFQLTRDNIKAIIMD 300

Query: 301 ---GSTRCCAEL 309
              G T   A +
Sbjct: 301 VMFGGTETVASV 312

BLAST of Csor.00g173480 vs. NCBI nr
Match: XP_022970686.1 (cytochrome P450 84A1-like [Cucurbita maxima])

HSP 1 Score: 584 bits (1505), Expect = 1.57e-206
Identity = 300/312 (96.15%), Postives = 303/312 (97.12%), Query Frame = 0

Query: 1   MESLNSSSFFFFFLVSLLFFLAFLWRLRRKLPYPPGPTGLPIIGNMLMMNELTHRGLTRL 60
           MESLNSSSFFFFFLVSLLFFLAFLWRLRRKLPYPPGPTGLPIIGNMLMMN+LTHRGLTRL
Sbjct: 1   MESLNSSSFFFFFLVSLLFFLAFLWRLRRKLPYPPGPTGLPIIGNMLMMNQLTHRGLTRL 60

Query: 61  AAIYGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFAN 120
           AAIYGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFAN
Sbjct: 61  AAIYGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFAN 120

Query: 121 YGPFWRQMRKICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGEPVNIGELVFGLTRNI 180
           YGPFWRQMRKICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGEPVNIGELVFGLTRNI
Sbjct: 121 YGPFWRQMRKICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGEPVNIGELVFGLTRNI 180

Query: 181 TYKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHAGEFNRRMEKARKSLD 240
           TYKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHAGEFNRRMEKARK+LD
Sbjct: 181 TYKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHAGEFNRRMEKARKALD 240

Query: 241 VFIDSIIDEHIAKKLGKNDDNDEQDVDSDMVDELMAFLVDESSSNDFQLTRDNIKAIIMS 300
           VFIDSIIDEHIAKKLGKNDDNDEQDVDSDMVDELMAFLVDESSSNDFQLTRDNIKAIIM 
Sbjct: 241 VFIDSIIDEHIAKKLGKNDDNDEQDVDSDMVDELMAFLVDESSSNDFQLTRDNIKAIIMD 300

Query: 301 ---GSTRCCAEL 309
              G T   A +
Sbjct: 301 VMFGGTETVASV 312

BLAST of Csor.00g173480 vs. NCBI nr
Match: XP_023531030.1 (cytochrome P450 84A1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 578 bits (1490), Expect = 2.99e-204
Identity = 297/312 (95.19%), Postives = 302/312 (96.79%), Query Frame = 0

Query: 1   MESLNSSSFFFFFLVSLLFFLAFLWRLRRKLPYPPGPTGLPIIGNMLMMNELTHRGLTRL 60
           MESLNSSSFFFFFLVSLLFFLAFLWRLRRKLPYPPGPTGLPIIGNMLMMNELTHRGLTRL
Sbjct: 1   MESLNSSSFFFFFLVSLLFFLAFLWRLRRKLPYPPGPTGLPIIGNMLMMNELTHRGLTRL 60

Query: 61  AAIYGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFAN 120
           AA+YGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFAN
Sbjct: 61  AAVYGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFAN 120

Query: 121 YGPFWRQMRKICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGEPVNIGELVFGLTRNI 180
           YGPFWRQMRKICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGE VNIGELVFGLTRNI
Sbjct: 121 YGPFWRQMRKICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGEAVNIGELVFGLTRNI 180

Query: 181 TYKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHAGEFNRRMEKARKSLD 240
           TYKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHAGEFNRRMEKARK+LD
Sbjct: 181 TYKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHAGEFNRRMEKARKALD 240

Query: 241 VFIDSIIDEHIAKKLGKNDDNDEQDVDSDMVDELMAFLVDESSSNDFQLTRDNIKAIIMS 300
           VFIDSIIDEHIAKKLGKN+DNDEQDV+SDMVDELMAFLVDESSSNDFQLTRDNIKAIIM 
Sbjct: 241 VFIDSIIDEHIAKKLGKNNDNDEQDVESDMVDELMAFLVDESSSNDFQLTRDNIKAIIMD 300

Query: 301 ---GSTRCCAEL 309
              G T   A +
Sbjct: 301 VMFGGTETVASV 312

BLAST of Csor.00g173480 vs. NCBI nr
Match: XP_022933285.1 (cytochrome P450 84A1-like [Cucurbita moschata])

HSP 1 Score: 578 bits (1489), Expect = 4.10e-204
Identity = 300/312 (96.15%), Postives = 301/312 (96.47%), Query Frame = 0

Query: 1   MESLNSSSFFFFFLVSLLFFLAFLWRLRRKLPYPPGPTGLPIIGNMLMMNELTHRGLTRL 60
           MESLNSSSFFFF LVSLLFFLAFLWRL RKLPYPPGPTGLPIIGNMLMMNELTHRGLTRL
Sbjct: 1   MESLNSSSFFFF-LVSLLFFLAFLWRLSRKLPYPPGPTGLPIIGNMLMMNELTHRGLTRL 60

Query: 61  AAIYGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFAN 120
           AAIYGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFAN
Sbjct: 61  AAIYGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFAN 120

Query: 121 YGPFWRQMRKICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGEPVNIGELVFGLTRNI 180
           YGPFWRQMRKICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGEPVNIGELVFGLTRNI
Sbjct: 121 YGPFWRQMRKICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGEPVNIGELVFGLTRNI 180

Query: 181 TYKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHAGEFNRRMEKARKSLD 240
           TYKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHAGEFNRRMEKARKSLD
Sbjct: 181 TYKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHAGEFNRRMEKARKSLD 240

Query: 241 VFIDSIIDEHIAKKLGKNDDNDEQDVDSDMVDELMAFLVDESSSNDFQLTRDNIKAIIMS 300
           VFIDSIIDEHIAKKLGKNDDNDEQDVDSDMVDELMAFLVDESSSNDFQLTRDNIKAIIM 
Sbjct: 241 VFIDSIIDEHIAKKLGKNDDNDEQDVDSDMVDELMAFLVDESSSNDFQLTRDNIKAIIMD 300

Query: 301 ---GSTRCCAEL 309
              G T   A +
Sbjct: 301 VMFGGTETVASV 311

BLAST of Csor.00g173480 vs. ExPASy TrEMBL
Match: A0A6J1I197 (cytochrome P450 84A1-like OS=Cucurbita maxima OX=3661 GN=LOC111469596 PE=3 SV=1)

HSP 1 Score: 584 bits (1505), Expect = 7.58e-207
Identity = 300/312 (96.15%), Postives = 303/312 (97.12%), Query Frame = 0

Query: 1   MESLNSSSFFFFFLVSLLFFLAFLWRLRRKLPYPPGPTGLPIIGNMLMMNELTHRGLTRL 60
           MESLNSSSFFFFFLVSLLFFLAFLWRLRRKLPYPPGPTGLPIIGNMLMMN+LTHRGLTRL
Sbjct: 1   MESLNSSSFFFFFLVSLLFFLAFLWRLRRKLPYPPGPTGLPIIGNMLMMNQLTHRGLTRL 60

Query: 61  AAIYGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFAN 120
           AAIYGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFAN
Sbjct: 61  AAIYGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFAN 120

Query: 121 YGPFWRQMRKICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGEPVNIGELVFGLTRNI 180
           YGPFWRQMRKICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGEPVNIGELVFGLTRNI
Sbjct: 121 YGPFWRQMRKICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGEPVNIGELVFGLTRNI 180

Query: 181 TYKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHAGEFNRRMEKARKSLD 240
           TYKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHAGEFNRRMEKARK+LD
Sbjct: 181 TYKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHAGEFNRRMEKARKALD 240

Query: 241 VFIDSIIDEHIAKKLGKNDDNDEQDVDSDMVDELMAFLVDESSSNDFQLTRDNIKAIIMS 300
           VFIDSIIDEHIAKKLGKNDDNDEQDVDSDMVDELMAFLVDESSSNDFQLTRDNIKAIIM 
Sbjct: 241 VFIDSIIDEHIAKKLGKNDDNDEQDVDSDMVDELMAFLVDESSSNDFQLTRDNIKAIIMD 300

Query: 301 ---GSTRCCAEL 309
              G T   A +
Sbjct: 301 VMFGGTETVASV 312

BLAST of Csor.00g173480 vs. ExPASy TrEMBL
Match: A0A6J1F4G7 (cytochrome P450 84A1-like OS=Cucurbita moschata OX=3662 GN=LOC111440398 PE=3 SV=1)

HSP 1 Score: 578 bits (1489), Expect = 1.98e-204
Identity = 300/312 (96.15%), Postives = 301/312 (96.47%), Query Frame = 0

Query: 1   MESLNSSSFFFFFLVSLLFFLAFLWRLRRKLPYPPGPTGLPIIGNMLMMNELTHRGLTRL 60
           MESLNSSSFFFF LVSLLFFLAFLWRL RKLPYPPGPTGLPIIGNMLMMNELTHRGLTRL
Sbjct: 1   MESLNSSSFFFF-LVSLLFFLAFLWRLSRKLPYPPGPTGLPIIGNMLMMNELTHRGLTRL 60

Query: 61  AAIYGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFAN 120
           AAIYGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFAN
Sbjct: 61  AAIYGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFAN 120

Query: 121 YGPFWRQMRKICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGEPVNIGELVFGLTRNI 180
           YGPFWRQMRKICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGEPVNIGELVFGLTRNI
Sbjct: 121 YGPFWRQMRKICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGEPVNIGELVFGLTRNI 180

Query: 181 TYKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHAGEFNRRMEKARKSLD 240
           TYKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHAGEFNRRMEKARKSLD
Sbjct: 181 TYKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHAGEFNRRMEKARKSLD 240

Query: 241 VFIDSIIDEHIAKKLGKNDDNDEQDVDSDMVDELMAFLVDESSSNDFQLTRDNIKAIIMS 300
           VFIDSIIDEHIAKKLGKNDDNDEQDVDSDMVDELMAFLVDESSSNDFQLTRDNIKAIIM 
Sbjct: 241 VFIDSIIDEHIAKKLGKNDDNDEQDVDSDMVDELMAFLVDESSSNDFQLTRDNIKAIIMD 300

Query: 301 ---GSTRCCAEL 309
              G T   A +
Sbjct: 301 VMFGGTETVASV 311

BLAST of Csor.00g173480 vs. ExPASy TrEMBL
Match: A0A0A0K0F0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G004720 PE=3 SV=1)

HSP 1 Score: 466 bits (1200), Expect = 2.51e-160
Identity = 246/320 (76.88%), Postives = 275/320 (85.94%), Query Frame = 0

Query: 1   MESLNSSSFFFFFLVSLLFFLAFLWRLRRKLPYPPGPTGLPIIGNMLMMNELTHRGLTRL 60
           ME LN  S FFF ++SLL F+ FL RL RKL YPPGPTGLPIIGNMLMM++LTHRGL RL
Sbjct: 1   MEFLNPLSLFFF-VLSLLLFVLFLRRLHRKLRYPPGPTGLPIIGNMLMMDQLTHRGLARL 60

Query: 61  AAIYGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFAN 120
           A IYGGLFHLRLGVLHMVVVSTP+MAREFLQVQD+AFANRPANVAISYLTYDRADMAFAN
Sbjct: 61  ATIYGGLFHLRLGVLHMVVVSTPDMAREFLQVQDVAFANRPANVAISYLTYDRADMAFAN 120

Query: 121 YGPFWRQMRKICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGEPVNIGELVFGLTRNI 180
           YGPFWRQMRKICV+KLFSRRRAESWASVRDEVD++++IVS KI +PVNIG+LVF LTRNI
Sbjct: 121 YGPFWRQMRKICVIKLFSRRRAESWASVRDEVDTLVEIVSRKIQQPVNIGDLVFSLTRNI 180

Query: 181 TYKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHAGEFNRRMEKARKSLD 240
           TYKAAFGS SHEGQDEFV+ILQEFSKLFGAFNIADFLPWLGWIHA EFN R+ KAR+ LD
Sbjct: 181 TYKAAFGSSSHEGQDEFVKILQEFSKLFGAFNIADFLPWLGWIHAREFNERLAKARRDLD 240

Query: 241 VFIDSIIDEHIAKKLGK-NDDNDEQDVDSDMVDELMAFLVDESSSNDF-------QLTRD 300
           VFIDSIIDEH+ KK+ K   +  + + D+DMVDELMAFLVD+SSSN+F       +LTRD
Sbjct: 241 VFIDSIIDEHLEKKMKKLKGEKVDDEEDTDMVDELMAFLVDDSSSNEFDDSQSVLKLTRD 300

Query: 301 NIKAIIMS---GSTRCCAEL 309
           +IKA+IM    G T   A +
Sbjct: 301 HIKALIMDVMFGGTETVASV 319

BLAST of Csor.00g173480 vs. ExPASy TrEMBL
Match: A0A1S3BID8 (cytochrome P450 84A1-like OS=Cucumis melo OX=3656 GN=LOC103490415 PE=3 SV=1)

HSP 1 Score: 457 bits (1175), Expect = 1.71e-156
Identity = 241/313 (77.00%), Postives = 267/313 (85.30%), Query Frame = 0

Query: 11  FFFLVSLLFFLAFLWRLRRKLPYPPGPTGLPIIGNMLMMNELTHRGLTRLAAIYGGLFHL 70
           FF   SLL F+ FL  LR KL YPPGPTGLPIIGNMLMM++LTHRGL  LA IYGGLFHL
Sbjct: 10  FFSFFSLLLFVVFLRSLRCKLRYPPGPTGLPIIGNMLMMDQLTHRGLAHLATIYGGLFHL 69

Query: 71  RLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFANYGPFWRQMRK 130
           RLG+LHMVVVSTP+MAREFLQVQDIAFANRPANVAISYLTYDRADMAFANYGPFWRQMRK
Sbjct: 70  RLGILHMVVVSTPDMAREFLQVQDIAFANRPANVAISYLTYDRADMAFANYGPFWRQMRK 129

Query: 131 ICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGEPVNIGELVFGLTRNITYKAAFGSCS 190
           ICV+KLFSRRR ESWASVRDEVD++++IVS+KI +PVNIG+LVF LTRNITYKAAFGS S
Sbjct: 130 ICVVKLFSRRRIESWASVRDEVDALIKIVSSKIQQPVNIGDLVFSLTRNITYKAAFGSSS 189

Query: 191 HEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHAGEFNRRMEKARKSLDVFIDSIIDEH 250
           HEGQDEFV+ILQEFSKLFGAFNIADFLPWLGWIHA EFN R+ KAR+ LD+FIDSIIDEH
Sbjct: 190 HEGQDEFVKILQEFSKLFGAFNIADFLPWLGWIHAREFNERLAKARRDLDMFIDSIIDEH 249

Query: 251 IAKKL----GKNDDNDEQDVDSDMVDELMAFLVDESSSNDF-------QLTRDNIKAIIM 309
           + KK+    GK  D++E   DSDMVDELMAFLVD+SSSN+F       +LTRDNIKA+IM
Sbjct: 250 LQKKMKKLKGKTVDDEE---DSDMVDELMAFLVDDSSSNEFDDSQSVLKLTRDNIKALIM 309

BLAST of Csor.00g173480 vs. ExPASy TrEMBL
Match: A0A6P4AD69 (cytochrome P450 84A1-like OS=Ziziphus jujuba OX=326968 GN=LOC107426594 PE=3 SV=1)

HSP 1 Score: 431 bits (1107), Expect = 2.33e-146
Identity = 226/315 (71.75%), Postives = 256/315 (81.27%), Query Frame = 0

Query: 1   MESLNSSSFFFFFLVSLLFFLAFLWRLRRKLPYPPGPTGLPIIGNMLMMNELTHRGLTRL 60
           ++SL SS F   F+   L F+  ++R R KLPYPPGP GLPIIGNM MM++LTHRGL   
Sbjct: 5   LQSLQSSPFSLLFIPLFLTFIL-IFRTRGKLPYPPGPKGLPIIGNMRMMDQLTHRGLAEQ 64

Query: 61  AAIYGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFAN 120
           A  YGGLFHLRLGVLHMVVVSTP+MARE LQVQD  F+NRPANVAISYLTYDRADMAFAN
Sbjct: 65  AKKYGGLFHLRLGVLHMVVVSTPKMAREVLQVQDGVFSNRPANVAISYLTYDRADMAFAN 124

Query: 121 YGPFWRQMRKICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGEPVNIGELVFGLTRNI 180
           YGPFWRQMRK+CVMKLFSRRRAESWASVR+EVD  ++ V+ KIG  VNIGELVF LTRNI
Sbjct: 125 YGPFWRQMRKVCVMKLFSRRRAESWASVREEVDDTVRTVAAKIGSAVNIGELVFALTRNI 184

Query: 181 TYKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHAGEFNRRMEKARKSLD 240
           TYKAAFGS SHEGQDEFV ILQEFSKLFGAFNIADF+PWLGW+HA EFNRRME+ARKSLD
Sbjct: 185 TYKAAFGSSSHEGQDEFVTILQEFSKLFGAFNIADFIPWLGWLHAQEFNRRMERARKSLD 244

Query: 241 VFIDSIIDEHIAKKLGKNDDNDEQDVDSDMVDELMAFL---VDESSSNDFQLTRDNIKAI 300
           VFID II +H+ KK  K +  ++ D ++DMVDEL+AFL   VD+ S + F LTRDNIKAI
Sbjct: 245 VFIDKIISDHMEKKKMKKNKTNDADAETDMVDELIAFLPDNVDQDSQSTFTLTRDNIKAI 304

Query: 301 IMS---GSTRCCAEL 309
           +M    G T   A +
Sbjct: 305 VMDVMFGGTETVASV 318

BLAST of Csor.00g173480 vs. TAIR 10
Match: AT4G36220.1 (ferulic acid 5-hydroxylase 1 )

HSP 1 Score: 383.6 bits (984), Expect = 1.5e-106
Identity = 191/307 (62.21%), Postives = 236/307 (76.87%), Query Frame = 0

Query: 1   MESLNSSSFFFFFLVSLLFFLAFLWRLRRKLPYPPGPTGLPIIGNMLMMNELTHRGLTRL 60
           +  L+  +     +VSL  F++F+ R RR+ PYPPGP G PIIGNMLMM++LTHRGL  L
Sbjct: 9   LSKLSDPTTSLVIVVSLFIFISFITR-RRRPPYPPGPRGWPIIGNMLMMDQLTHRGLANL 68

Query: 61  AAIYGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFAN 120
           A  YGGL HLR+G LHM  VS+PE+AR+ LQVQD  F+NRPA +AISYLTYDRADMAFA+
Sbjct: 69  AKKYGGLCHLRMGFLHMYAVSSPEVARQVLQVQDSVFSNRPATIAISYLTYDRADMAFAH 128

Query: 121 YGPFWRQMRKICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGEPVNIGELVFGLTRNI 180
           YGPFWRQMRK+CVMK+FSR+RAESWASVRDEVD +++ VS  +G+P+N+GE +F LTRNI
Sbjct: 129 YGPFWRQMRKVCVMKVFSRKRAESWASVRDEVDKMVRSVSCNVGKPINVGEQIFALTRNI 188

Query: 181 TYKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHAGEFNRRMEKARKSLD 240
           TY+AAFGS   +GQDEF+ ILQEFSKLFGAFN+ADF+P+ GWI     N+R+ KAR  LD
Sbjct: 189 TYRAAFGSACEKGQDEFIRILQEFSKLFGAFNVADFIPYFGWIDPQGINKRLVKARNDLD 248

Query: 241 VFIDSIIDEHIAKKLGKNDDNDEQDVDSDMVDELMAFLVDESS--------SNDFQLTRD 300
            FID IIDEH+ KK  +N  +D   VD+DMVD+L+AF  +E+          N  +LTRD
Sbjct: 249 GFIDDIIDEHMKKKENQNAVDDGDVVDTDMVDDLLAFYSEEAKLVSETADLQNSIKLTRD 308

BLAST of Csor.00g173480 vs. TAIR 10
Match: AT5G04330.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 321.2 bits (822), Expect = 8.9e-88
Identity = 166/296 (56.08%), Postives = 220/296 (74.32%), Query Frame = 0

Query: 14  LVSLLFFLAFLWRLRRKL---PYPPGPTGLPIIGNMLMMNELTHRGLTRLAAIYGGLFHL 73
           LV LL FL     LRR++   PYPPGP GLP+IGN+LMMN+  HRGL +L+ IYGGL HL
Sbjct: 10  LVPLLLFLFPHLLLRRQMLLKPYPPGPKGLPVIGNILMMNQFNHRGLAKLSRIYGGLLHL 69

Query: 74  RLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFANYGPFWRQMRK 133
           RLG  H+ VVS+P++AR+ LQVQD  F+NRP  +AI YLTY  +D+AF NYGPFWR+MRK
Sbjct: 70  RLGFSHIFVVSSPDIARQVLQVQDHVFSNRPTTIAIRYLTYGGSDLAFCNYGPFWRRMRK 129

Query: 134 ICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGEPVNIGELVFGLTRNITYKAAFGSCS 193
           + VM LFSR+RAESW SV +EV   +++V++ +G+P+NI +L F L+R+IT++AAFGS S
Sbjct: 130 LYVMMLFSRKRAESWVSVDEEVHKSVRLVASNVGKPLNICKLAFSLSRDITFRAAFGSSS 189

Query: 194 HEGQ----DEFVEILQEFSKLFGAFNIADFLP-WLGWIHAGEFNRRMEKARKSLDVFIDS 253
                   DEF+EI+QEFSKLFG FN+AD++P WL WI     N R+EKARKSLD FI+S
Sbjct: 190 STSDESRLDEFLEIIQEFSKLFGEFNVADYVPSWLSWIDPQGINGRVEKARKSLDGFIES 249

Query: 254 IIDEHIAKKLGKNDDNDEQDVDSDMVDELMAFLVDESSSND--FQLTRDNIKAIIM 300
           +ID+H+ KK  ++D+ DE   ++DMVD+L+AF  +E   N+   ++  DNIK IIM
Sbjct: 250 VIDDHLHKKKREHDNVDE---ETDMVDQLLAFYEEEVKVNNSVTKINLDNIKGIIM 302

BLAST of Csor.00g173480 vs. TAIR 10
Match: AT3G48270.1 (cytochrome P450, family 71, subfamily A, polypeptide 26 )

HSP 1 Score: 171.0 bits (432), Expect = 1.5e-42
Identity = 99/299 (33.11%), Postives = 167/299 (55.85%), Query Frame = 0

Query: 11  FFFLVSLLFFLAFL-------WRLRRKLPYPPGPTGLPIIGNMLMMNELTHRGLTRLAAI 70
           FF L S++F +  +        + R  LP PP   GLP+IGN+  +    HR L  L+  
Sbjct: 5   FFLLCSIIFVVTIIIFRKQKRGKKRNTLPSPP---GLPLIGNLHQLGRHPHRSLCSLSHR 64

Query: 71  YGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVAISYLTYDRADMAFANYGP 130
           YG L  L  G + ++VVS+ E+AR+ L+  D  FA+RP +     L YD+ D+A A YG 
Sbjct: 65  YGPLMLLHFGRVPVLVVSSAELARDVLKTHDRVFASRPRSKIFEKLLYDKHDVASAPYGE 124

Query: 131 FWRQMRKICVMKLFSRRRAESWASVR-DEVDSVLQIVSNKIGEPVNIGELVFGLTRNITY 190
           +WRQM+ +CV+ LFS +   S+  VR +E+  +++ +   I  PVN+ +++  LT ++  
Sbjct: 125 YWRQMKSVCVLHLFSNKMVRSFREVREEEISLMMEKIRKSISLPVNLSKILVSLTNDVIC 184

Query: 191 KAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIH-AGEFNRRMEKARKSLDV 250
           K A G   + G+ +F E+++  +KL G F++  ++PWL WI      + ++EK    +D 
Sbjct: 185 KVALGR-KYGGETDFKELMERLNKLLGTFSVGSYVPWLAWIDWIRGLDCQLEKTANDVDK 244

Query: 251 FIDSIIDEHIAKKLGKNDDNDEQDVDSDMVDELMAFLVDESSSNDFQLTRDNIKAIIMS 301
           F + ++ +H+       D N +    +D VD L+A   D++    F++ R +IKAI+M+
Sbjct: 245 FFERVVQDHV-------DGNRDM---TDFVDVLLAIQRDKTVG--FEINRVSIKAIVMN 287

BLAST of Csor.00g173480 vs. TAIR 10
Match: AT5G06900.1 (cytochrome P450, family 93, subfamily D, polypeptide 1 )

HSP 1 Score: 164.5 bits (415), Expect = 1.4e-40
Identity = 100/283 (35.34%), Postives = 160/283 (56.54%), Query Frame = 0

Query: 26  RLRRKLPYPPGPTGLPIIGNMLMMNELTHRGLTRLAAIYGGLFHLRLGVLHMVVVSTPEM 85
           RLR +LP PP PT LPIIG++ ++  + H+ L +L+  YG L +L +G +  ++VS+ EM
Sbjct: 27  RLRDRLPLPPSPTALPIIGHIHLLGPIAHQALHKLSIRYGPLMYLFIGSIPNLIVSSAEM 86

Query: 86  AREFLQVQDIAFANRPANVAISYLTYDRADMAFANYGPFWRQMRKICVMKLFSRRRAESW 145
           A E L+  ++ F NRP    + YLTY  AD   A YG  W+ M++IC+++LFS R  +S+
Sbjct: 87  ANEILKSNELNFLNRPTMQNVDYLTYGSADFFSAPYGLHWKFMKRICMVELFSSRALDSF 146

Query: 146 ASVR-DEVDSVLQIVSNK--IGEPVNIGELVFGLTRNITYKAAF-----GSCSHEGQDEF 205
            SVR +E+  +L  V  K    E VN+GE +  LT NI  +  F      S   E  +E 
Sbjct: 147 VSVRSEELKKLLIRVLKKAEAEESVNLGEQLKELTSNIITRMMFRKMQSDSDGGEKSEEV 206

Query: 206 VEILQEFSKLFGAFNIADFLPWLGWIHAGEFNRRMEKARKSLDVFIDSIIDEHIAKKLGK 265
           ++++ E ++L G FN+++   +L  +      +R++ AR   DV I+ I++EH + K   
Sbjct: 207 IKMVVELNELAGFFNVSETFWFLKRLDLQGLKKRLKNARDKYDVIIERIMEEHESSKKNA 266

Query: 266 NDDNDEQDVDSDMVDELMAFLVDESSSNDFQLTRDNIKAIIMS 301
             + +  DV  D         + E  + + +LTR+NIKA IM+
Sbjct: 267 TGERNMLDVLLD---------IYEDKNAEMKLTRENIKAFIMN 300

BLAST of Csor.00g173480 vs. TAIR 10
Match: AT3G48280.1 (cytochrome P450, family 71, subfamily A, polypeptide 25 )

HSP 1 Score: 164.1 bits (414), Expect = 1.8e-40
Identity = 91/268 (33.96%), Postives = 151/268 (56.34%), Query Frame = 0

Query: 34  PPGPTGLPIIGNMLMMNELTHRGLTRLAAIYGGLFHLRLGVLHMVVVSTPEMAREFLQVQ 93
           PP P GLP+IGN+  +   THR L  L+  YG L  L LG + +++VS+ +MA+E L+  
Sbjct: 31  PPSPPGLPLIGNLHQLGRHTHRSLCDLSRRYGPLMLLHLGRVPVLIVSSADMAQEILKTH 90

Query: 94  DIAFANRPANVAISYLTYDRADMAFANYGPFWRQMRKICVMKLFSRRRAESWASVRDEVD 153
           D AFANRP +     L Y+  D+A A YG +WRQM+ +CV+ L S +   S+  VR+E  
Sbjct: 91  DQAFANRPRSKLSQKLLYNNRDVASAPYGEYWRQMKSVCVIHLLSNKMVRSFRDVREEEI 150

Query: 154 SVLQIVSNKIGE-PVNIGELVFGLTRNITYKAAFGSCSHEGQDEFVEILQEFSKLFGAFN 213
           +++     K    P N+ +++  LT ++  + A G   + G+ +F ++    S+L G F+
Sbjct: 151 TLMMAKIRKSSSLPFNVSKVLECLTNDVICRVALGR-KYGGETDFKKLTDRLSELLGTFS 210

Query: 214 IADFLPWLGWIH-AGEFNRRMEKARKSLDVFIDSIIDEHIAKKLGKNDDNDEQDVDSDMV 273
           I  F+PWL W+     ++ +++K  K LD F + ++ +H        +D D +D  +D++
Sbjct: 211 IGSFVPWLAWVDWIRGWDAQLDKMGKDLDDFFEKVVQDH--------EDGDRRD-GTDLI 270

Query: 274 DELMAFLVDESSSNDFQLTRDNIKAIIM 300
           D L+   V    S  F++ R +IKAI +
Sbjct: 271 DALLR--VKREKSPGFEIERVSIKAITL 286

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q426002.1e-10562.21Cytochrome P450 84A1 OS=Arabidopsis thaliana OX=3702 GN=CYP84A1 PE=1 SV=1[more]
F4JW831.3e-8656.08Cytochrome P450 84A4 OS=Arabidopsis thaliana OX=3702 GN=CYP84A4 PE=1 SV=1[more]
A6YIH85.2e-4839.60Premnaspirodiene oxygenase OS=Hyoscyamus muticus OX=35626 GN=CYP71D55 PE=1 SV=1[more]
W8JCE82.2e-4638.41Cytochrome P450 71BT1 OS=Catharanthus roseus OX=4058 GN=CYP71BT1 PE=2 SV=1[more]
P244651.1e-4537.50Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
KAG6588421.11.36e-220100.00Cytochrome P450 84A1, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7022266.11.92e-20796.79Cytochrome P450 84A1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022970686.11.57e-20696.15cytochrome P450 84A1-like [Cucurbita maxima][more]
XP_023531030.12.99e-20495.19cytochrome P450 84A1-like [Cucurbita pepo subsp. pepo][more]
XP_022933285.14.10e-20496.15cytochrome P450 84A1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1I1977.58e-20796.15cytochrome P450 84A1-like OS=Cucurbita maxima OX=3661 GN=LOC111469596 PE=3 SV=1[more]
A0A6J1F4G71.98e-20496.15cytochrome P450 84A1-like OS=Cucurbita moschata OX=3662 GN=LOC111440398 PE=3 SV=... [more]
A0A0A0K0F02.51e-16076.88Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G004720 PE=3 SV=1[more]
A0A1S3BID81.71e-15677.00cytochrome P450 84A1-like OS=Cucumis melo OX=3656 GN=LOC103490415 PE=3 SV=1[more]
A0A6P4AD692.33e-14671.75cytochrome P450 84A1-like OS=Ziziphus jujuba OX=326968 GN=LOC107426594 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G36220.11.5e-10662.21ferulic acid 5-hydroxylase 1 [more]
AT5G04330.18.9e-8856.08Cytochrome P450 superfamily protein [more]
AT3G48270.11.5e-4233.11cytochrome P450, family 71, subfamily A, polypeptide 26 [more]
AT5G06900.11.4e-4035.34cytochrome P450, family 93, subfamily D, polypeptide 1 [more]
AT3G48280.11.8e-4033.96cytochrome P450, family 71, subfamily A, polypeptide 25 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PFAMPF00067p450coord: 34..299
e-value: 2.5E-30
score: 105.7
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 23..301
e-value: 1.6E-49
score: 170.7
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 26..299
NoneNo IPR availablePANTHERPTHR47945:SF6CYTOCHROME P450 FAMILY MONOOXYGENASEcoord: 7..300
NoneNo IPR availablePANTHERPTHR47945CYTOCHROME P450 84A1-RELATEDcoord: 7..300

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g173480.m01Csor.00g173480.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009809 lignin biosynthetic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen