Csor.00g173140 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g173140
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionAuxin efflux carrier component
LocationCsor_Chr11: 5990211 .. 5992888 (+)
RNA-Seq ExpressionCsor.00g173140
SyntenyCsor.00g173140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTTCGTTGTCTGATGTGTATCATGTTGTGGCAGCCACAGTGCCACTGTATGTGGCCATGATATTAGCTTACATATCAGTAAGATGGTGGAAGTTCTTCACAGCAGAGCAATGTTGTGGAATAAACAAGTTTGTGGCGAAATTCTCAATCCCACTGCTATCATTTCAAGTCATTTCAGACAACAATCTCTACAAAATGAACCTGCAATTGATATTGGCTGACTTTGTTCAGAAGATATTAGCTATTTCTCTGATGGGAGCAGTGACTAAAATCACGTCTAGGGGAGGGTTGAACTGGATAATCACAGGGCTTTCTCTGTCCACCATGCCTAATACTCTCATTCTTGGGCTGCCTGTATTGAAGGCCATGTATGGCAGTGAAGCAGATGTGCTTCTTGCCCAGATTGTTGTCTTGCAGAGCGTTGTTTGGTATAATATATTGTTGTTTCTGTTTGAGTTTACTACCACTAAAGCAGCTGCTTTAGCTCCAGCATCAGAAGCCATAGGTAAGGATTGGAATTTGGTTTTTGAATCGAATTATCTCAACTTTTTTAAAAAATGTTTTGTTCTCGTTTTCCTCGACTTTTGGGGTCCAGCGTTCTCGCTGGCACTCGTTTCCCTCTCCAATTGATGTGGGATCTCACAAACCACCCCGTTGGGCCTAGCGTCCTTGCTGGCACACTATTCGGTGTCTGGCTTTGATACCAATTGTAACAGCCCAAGACCACTGCTAGCCGATATTGCCCTATTTCGACTACCTTCCGAGCTTCCCTTCAAGGTTTTTAAAATGCATCCGTTAGGAAGAGGTTTCTACACCCTTATAAAGAATGATTTGTTCTCTTCCCCAACCGATGTGGAATCTCACAATCCACTGTTAGAAGTCACGGATCTCCACAATGGTATGATATTGTTCACTTTAAGCATAAGCTCTCGTGGCTTTGCTTTGGGCTTCCCCAAAAGGCCTTATACCAATGGAGATAGTATTCCTCCCTTAGAAACCCATATGATCATTTCCTAAATTAGCCAACGTGGGACTCATTCCCAATAATTCTCAACGTCCACCCTGTAGATCTAGCGTCCTCGTTGACACTCGTTCCCCCTCTTCAATCAATGTCGTTCTCCTCTCCCATCGATGTGGGATCTCACAATCCACCACCCTTCGGGGCCCAGAATCCTCGCTGACACACTACCCGGTGTCTAGCTCTAATACCATTTGTAACAACCCAAGCCCACCACTAGGAGATATTGTTCTCTTTACCCTTTCCCTTTTCAACTTCCTCTCAAGGTTTTTAAAACACGTCTATTAGGAAGAGGTTTCCACACTTTTATAAACAATGCTTCGTTCTCCTCCCAACCAACGTGGGATCTCACAATCCATCCCCTTCAGGACTAGTGTTCTCGTTGGCACTCGTTTCCCTCTCCAATCGATATGCGATCTCACTGTGTTTATCTTTCAGAAATTGATACAACTGAAGAAGGATTAGCCAAAGAAGAAGGTGAGGAAGCAAGAGACAGCAATGGAAGGAGGCACAAAACCAGGTCCATTCTCTTCACAGTTGCTAGGAAGCTCATCTACAACCCCAACACTCATGCAACAATATTAGGACTCATTTGGGCAAGCATAAGATTCAGGTAAACCAAACACAATACCTCTGCTCTAACTTCACACATGTTTGGGGATGAAACTAACATACTCTCAGGTGGGGAGTAAAACTACCAGAAGTAATAGATCGATCCATAACAATTCTGTCAACTGGAGGACTTGGTATGGCCATGTTCAGCTTAGGCCTTTTCATGGGTTCACGGACAAGCATCATAGCATGCGGTACAAAGATGACACTGGTAGCCATGGGAATGAAATTCTTAATGGGACCTGCACTTATGGCTGCATCCTCACTTCCTCTAGGCCTCAAGGGCAGATTGCTCCGAGTGGCAATCGTGCAGGTTTGTTTTAATTTGCATCACCAGTTGTGAGATCCCACATCGATTGTGGAGGAGAACGAAACGCCCTTGATAAGGGTGTGGAAACCTCTCCCTAGTGGACGCGTTTTAAAAACCTTGAGGGAAAGTCTGAAACCTCTCCCAATATCTATTAGTGGTGGGTCTGGGCCGTTACAAATGGTATCAAATCCCGACATTGGGCGATGTGCCAGTAAGGAAGCTGTTCCCTGAAGGGAGTAGACATGAGGCGGTGTGTCAGTAAGGACGCTGGTCTCCAAAGGGGGTGGATTTGGTAAAGGTCCCACATCGATTAGAGAAAGGAACAAGTATCAACGAAAACGTTGGGCCCTGAAAGAGGGTGGATTGTGAGATCCCACATTGGTTGGAGAGGAGAACGGAAACACCCTTTATCAAACGCGTTTTAAAAACCTTGAAAGAAAGTCTAAAAAGGACAATATCTGCTGCTAGCGGACCTAGGCCACTAGGATCGACAATTTTGTTTGATTGTTTGTTATTTGGTACAGGCAGCACTTCCTCAAGGCATTGTTCCATTTGTGTTTGCCAAAGAGTATAATATACACCCAGATGTCTTAAGCACAGGGTAACTCTTCTAACATTCATCTCGTGGCCTCAACTAGTACTGAAATGGGGCTTTGTTTTGGTGTCAGGGTACTTCTAGGCTTGCTCGTTGCATTGCCAGTAGCATTGGCTTATTACTCCTTGCTATCATTCTAA

mRNA sequence

ATGATTTCGTTGTCTGATGTGTATCATGTTGTGGCAGCCACAGTGCCACTGTATGTGGCCATGATATTAGCTTACATATCAGTAAGATGGTGGAAGTTCTTCACAGCAGAGCAATGTTGTGGAATAAACAAGTTTGTGGCGAAATTCTCAATCCCACTGCTATCATTTCAAGTCATTTCAGACAACAATCTCTACAAAATGAACCTGCAATTGATATTGGCTGACTTTGTTCAGAAGATATTAGCTATTTCTCTGATGGGAGCAGTGACTAAAATCACGTCTAGGGGAGGGTTGAACTGGATAATCACAGGGCTTTCTCTGTCCACCATGCCTAATACTCTCATTCTTGGGCTGCCTGTATTGAAGGCCATGTATGGCAGTGAAGCAGATGTGCTTCTTGCCCAGATTGTTGTCTTGCAGAGCGTTGTTTGGTATAATATATTGTTGTTTCTGTTTGAGTTTACTACCACTAAAGCAGCTGCTTTAGCTCCAGCATCAGAAGCCATAGAAATTGATACAACTGAAGAAGGATTAGCCAAAGAAGAAGGTGAGGAAGCAAGAGACAGCAATGGAAGGAGGCACAAAACCAGGTCCATTCTCTTCACAGTTGCTAGGAAGCTCATCTACAACCCCAACACTCATGCAACAATATTAGGACTCATTTGGGCAAGCATAAGATTCAGAAGTAATAGATCGATCCATAACAATTCTGTCAACTGGAGGACTTGGTATGGCCATGTTCAGCTTAGGCCTTTTCATGGGTTCACGGACAAGCATCATAGCATGCGGTACAAAGATGACACTGGTAGCCATGGGAATGAAATTCTTAATGGGACCTGCACTTATGGCTGCATCCTCACTTCCTCTAGGCCTCAAGGGCAGATTGCTCCGAGTGGCAATCGTGCAGCACTTCCTCAAGGCATTGTTCCATTTGTGTTTGCCAAAGAGTATAATATACACCCAGATGTCTTAAGCACAGGGGTACTTCTAGGCTTGCTCGTTGCATTGCCAGTAGCATTGGCTTATTACTCCTTGCTATCATTCTAA

Coding sequence (CDS)

ATGATTTCGTTGTCTGATGTGTATCATGTTGTGGCAGCCACAGTGCCACTGTATGTGGCCATGATATTAGCTTACATATCAGTAAGATGGTGGAAGTTCTTCACAGCAGAGCAATGTTGTGGAATAAACAAGTTTGTGGCGAAATTCTCAATCCCACTGCTATCATTTCAAGTCATTTCAGACAACAATCTCTACAAAATGAACCTGCAATTGATATTGGCTGACTTTGTTCAGAAGATATTAGCTATTTCTCTGATGGGAGCAGTGACTAAAATCACGTCTAGGGGAGGGTTGAACTGGATAATCACAGGGCTTTCTCTGTCCACCATGCCTAATACTCTCATTCTTGGGCTGCCTGTATTGAAGGCCATGTATGGCAGTGAAGCAGATGTGCTTCTTGCCCAGATTGTTGTCTTGCAGAGCGTTGTTTGGTATAATATATTGTTGTTTCTGTTTGAGTTTACTACCACTAAAGCAGCTGCTTTAGCTCCAGCATCAGAAGCCATAGAAATTGATACAACTGAAGAAGGATTAGCCAAAGAAGAAGGTGAGGAAGCAAGAGACAGCAATGGAAGGAGGCACAAAACCAGGTCCATTCTCTTCACAGTTGCTAGGAAGCTCATCTACAACCCCAACACTCATGCAACAATATTAGGACTCATTTGGGCAAGCATAAGATTCAGAAGTAATAGATCGATCCATAACAATTCTGTCAACTGGAGGACTTGGTATGGCCATGTTCAGCTTAGGCCTTTTCATGGGTTCACGGACAAGCATCATAGCATGCGGTACAAAGATGACACTGGTAGCCATGGGAATGAAATTCTTAATGGGACCTGCACTTATGGCTGCATCCTCACTTCCTCTAGGCCTCAAGGGCAGATTGCTCCGAGTGGCAATCGTGCAGCACTTCCTCAAGGCATTGTTCCATTTGTGTTTGCCAAAGAGTATAATATACACCCAGATGTCTTAAGCACAGGGGTACTTCTAGGCTTGCTCGTTGCATTGCCAGTAGCATTGGCTTATTACTCCTTGCTATCATTCTAA

Protein sequence

MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQVISDNNLYKMNLQLILADFVQKILAISLMGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPVLKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTKAAALAPASEAIEIDTTEEGLAKEEGEEARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLIWASIRFRSNRSIHNNSVNWRTWYGHVQLRPFHGFTDKHHSMRYKDDTGSHGNEILNGTCTYGCILTSSRPQGQIAPSGNRAALPQGIVPFVFAKEYNIHPDVLSTGVLLGLLVALPVALAYYSLLSF
Homology
BLAST of Csor.00g173140 vs. ExPASy Swiss-Prot
Match: Q9LFP6 (Auxin efflux carrier component 8 OS=Arabidopsis thaliana OX=3702 GN=PIN8 PE=2 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 5.0e-68
Identity = 170/370 (45.95%), Postives = 227/370 (61.35%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQVIS 60
           MIS  D+YHVV+ATVPLYV+M L ++S R  K F+ EQC GINKFVAKFSIPLLSFQ+IS
Sbjct: 1   MISWLDIYHVVSATVPLYVSMTLGFLSARHLKLFSPEQCAGINKFVAKFSIPLLSFQIIS 60

Query: 61  DNNLYKMNLQLILADFVQKILAISLMGAVTKI----TSRGG-LNWIITGLSLSTMPNTLI 120
           +NN +KM+ +LIL+D +QK L + ++  V +       RGG L W+ITGLS+S +PNTLI
Sbjct: 61  ENNPFKMSPKLILSDILQKFLVVVVLAMVLRFWHPTGGRGGKLGWVITGLSISVLPNTLI 120

Query: 121 LGLPVLKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTKAAALAPAS------EAI 180
           LG+P+L A+YG EA  +L QIVVLQS++WY ILLFLFE    +A   + AS      +  
Sbjct: 121 LGMPILSAIYGDEAASILEQIVVLQSLIWYTILLFLFELNAARALPSSGASLEHTGNDQE 180

Query: 181 EIDTTEEGLAKEEGEEARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLIWASIRFR- 240
           E +  +E   +E+ EE      R   T  IL    RKLI NPNT+AT++G+IWA++ FR 
Sbjct: 181 EANIEDEPKEEEDEEEVAIVRTRSVGTMKILLKAWRKLIINPNTYATLIGIIWATLHFRL 240

Query: 241 -------SNRSIHNNSVNWRTWYGHVQLRPFHGFTDKHHSMRYKDDTGSHGNEILNG--- 300
                   ++SIH  S +         L  F        +   K    +   + + G   
Sbjct: 241 GWNLPEMIDKSIHLLS-DGGLGMAMFSLGLFMASQSSIIACGTKMAIITMLLKFVLGPAL 300

Query: 301 --TCTYGCILTSSRPQGQIAPSGNRAALPQGIVPFVFAKEYNIHPDVLSTGVLLGLLVAL 347
                Y   L S+  +  I     +AALPQG+VPFVFAKEYN+HP+++STGV+ G+L+AL
Sbjct: 301 MIASAYCIRLKSTLFKVAIL----QAALPQGVVPFVFAKEYNLHPEIISTGVIFGMLIAL 360

BLAST of Csor.00g173140 vs. ExPASy Swiss-Prot
Match: Q0JJV0 (Probable auxin efflux carrier component 8 OS=Oryza sativa subsp. japonica OX=39947 GN=PIN8 PE=2 SV=2)

HSP 1 Score: 228.4 bits (581), Expect = 1.2e-58
Identity = 149/351 (42.45%), Postives = 194/351 (55.27%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQVIS 60
           M+S  D+Y V+ ATVPLYVAMILAY+S++WWK FT EQC GINKFVAKFSIPLLSFQVIS
Sbjct: 1   MVSWKDIYLVLEATVPLYVAMILAYLSIKWWKLFTPEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  DNNLYKMNLQLILADFVQKILAISLMGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
             + Y MN++LI +D +QK LA+    A++K       +W+ITG SLST+PNTLI+G+P+
Sbjct: 61  TTDPYDMNIKLIYSDILQKSLALLGFAAISKACCAEKFDWLITGFSLSTLPNTLIVGIPL 120

Query: 121 LKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTKAAALAPASEAIEIDTTEEGLAK 180
           LK MYG +A  LL+QIVVLQS++WY +LLFLFE       A   +SE             
Sbjct: 121 LKGMYGEQAGKLLSQIVVLQSLIWYTLLLFLFELRAANGMATTTSSET------------ 180

Query: 181 EEGEEARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLIWASIRFRSN---RSIHNNS 240
                                                 GLIWA + FR +     I +NS
Sbjct: 181 -------------------------------------TGLIWALVGFRWHIRLPLIVSNS 240

Query: 241 VNWRTWYG----HVQLRPFHGFTDKHHSMRYKDDTGSHGNEILNGTCTYGCILTSSRPQG 300
           +   +  G       L  F     K  +   K    +       G    G    +   +G
Sbjct: 241 IRMLSDGGLGMAMFSLGLFTALQTKIIACGAKRMLLALAIRFFLGPALMGMSSYAIGMRG 300

Query: 301 QIAPSG-NRAALPQGIVPFVFAKEYNIHPDVLSTGVLLGLLVALPVALAYY 344
            +      +AALPQGIVPFVFAKEYN+  D+LST +++G++VA+PVALAYY
Sbjct: 301 VLLKIAIVQAALPQGIVPFVFAKEYNVQADILSTAIIVGMMVAVPVALAYY 302

BLAST of Csor.00g173140 vs. ExPASy Swiss-Prot
Match: Q5VQY3 (Probable auxin efflux carrier component 9 OS=Oryza sativa subsp. japonica OX=39947 GN=PIN9 PE=2 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 3.5e-45
Identity = 146/429 (34.03%), Postives = 218/429 (50.82%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQVIS 60
           MI+ S+VY VV A  PLY A  L Y SVRW K F+ EQC GIN FVA +++P+L F ++S
Sbjct: 1   MITGSEVYQVVEAMAPLYTAAALGYGSVRWLKAFSNEQCAGINHFVALYAVPVLIFDMVS 60

Query: 61  DNNLYKMNLQLILADFVQK-ILAISLM-------------GAVTKITSRGGLNWIITGLS 120
            NN+YKMN +LI AD +QK +L + LM             GA  K      L W+IT  S
Sbjct: 61  TNNVYKMNGRLIAADTLQKAVLLLGLMAWALWERSRARGAGAKAKAAVSSPLQWVITCFS 120

Query: 121 LSTMPNTLILGLPVLKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEF------------ 180
           ++++PNT+I+G+P+L  MYG  +  L+ QIVV+Q  +WYN+++FL+E+            
Sbjct: 121 VASLPNTIIMGVPLLNGMYGPVSKDLMKQIVVMQFCIWYNVIIFLYEYMAARRSASAPPP 180

Query: 181 ------------TTTKAAALA------------PASEAIEIDTTE-------EGLAKE-- 240
                       +  KAAA A            P   A+ I+ TE       +G++ E  
Sbjct: 181 ASSEGSAKISPSSPVKAAAAAADTNGNAVAADRPQEVAVNIEITEMAASTARDGVSGETT 240

Query: 241 -------EGE----EARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLIWASIRFRSN 300
                   GE    E  +++      + +++   +KL+  PNT+A+ LGLIW+ I F+  
Sbjct: 241 AAAKEVSSGEVAPVEEEEASAPAPSMKHVIWMAVKKLLQIPNTYASFLGLIWSLIAFKCG 300

Query: 301 RS----IHNNSVNWRTWYGHVQLRPFHGFTDKHHSMR-----YKDDTGSHGNEILNGTCT 347
            S    + ++    RT    V L  F   T      R     YK  + S   + L G   
Sbjct: 301 FSMPKIVEDSLFTIRT--TAVGLSMFSSGTFIARQSRFVPCGYKIASFSMVIKFLIGPVV 360

BLAST of Csor.00g173140 vs. ExPASy Swiss-Prot
Match: Q5JLM1 (Probable auxin efflux carrier component 5a OS=Oryza sativa subsp. japonica OX=39947 GN=PIN5A PE=2 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 7.8e-45
Identity = 130/370 (35.14%), Postives = 200/370 (54.05%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQVIS 60
           MI   DVY VVAATVPLY A+ L Y SVRWW+ FT EQC  +N+ VA F++P  +F+   
Sbjct: 1   MIGWGDVYKVVAATVPLYFALFLGYGSVRWWRIFTREQCDAVNRLVAFFALPFFTFEFTL 60

Query: 61  DNNLYKMNLQLILADFVQKILAISLMGAVTKITSRGG--LNWIITGLSLSTMPNTLILGL 120
             + +++N + + AD + K + ++++GA  +  S+GG  ++W IT  SLST+ N+L++G+
Sbjct: 61  HTDPFQVNYRAVAADVISKAVIVAVIGAWARFMSKGGCAVSWSITSFSLSTLTNSLVVGV 120

Query: 121 PVLKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTKAAAL------APASEAIEID 180
           P+ +AMYG  A  L+ Q+ V Q++VW  +LLF+ E    + AA+      A A+ A   D
Sbjct: 121 PMARAMYGEWAQQLVVQLSVFQAIVWLTLLLFVLE---VRKAAIGMYVDGAEAAAAAGKD 180

Query: 181 TTEEGLAKEEGEEARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLIWASIRFRSNRS 240
               G A   G     +   +    +++  VA KL  NPNT+A+ +G+ WA +  R + +
Sbjct: 181 VEAAGAAAAAGTVVVAAAAGKPSLWALVKVVAHKLARNPNTYASFVGITWACLANRLHIA 240

Query: 241 IHNNSVNWRTWYGHVQLRPFHGFTDKHHSMRYKDDTGSHGNEILNGT--CTYGCIL---- 300
           + +       + G V +    G      SM            I  GT     G +L    
Sbjct: 241 LPS------AFEGSVLIMSKSGTGMAMFSMGL--FMAQQEKIIACGTSFAALGLVLKFAL 300

Query: 301 -TSSRPQGQIAPS--GN-------RAALPQGIVPFVFAKEYNIHPDVLSTGVLLGLLVAL 347
             ++   G IA    G+       +AALPQ I  F+FAKEY +H DVLST V+ G+LV+L
Sbjct: 301 GPAAMAIGSIAVGLRGDVLRVAIIQAALPQSITSFIFAKEYGLHADVLSTAVIFGMLVSL 359

BLAST of Csor.00g173140 vs. ExPASy Swiss-Prot
Match: P0C0X5 (Probable auxin efflux carrier component 1b OS=Oryza sativa subsp. japonica OX=39947 GN=PIN1B PE=2 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 3.9e-44
Identity = 157/552 (28.44%), Postives = 228/552 (41.30%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQVIS 60
           MI++ D+YHV+ A VPLYVAM LAY SVRWW+ F+ +QC GIN+FVA F++PLLSF  IS
Sbjct: 1   MITVVDLYHVLTAVVPLYVAMTLAYASVRWWRIFSPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  DNNLYKMNLQLILADFVQKILAISLMGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
            NN + MNL+ + AD +QK++ ++L+    ++++RG L+W+IT  SLST+PNTL++G+P+
Sbjct: 61  TNNPFAMNLRFLAADTLQKLIVLALLALWCRLSARGSLDWLITLFSLSTLPNTLVMGIPL 120

Query: 121 LKAMYGSEADV----LLAQIVVLQSVVWYNILLFLFEFTTTK------------------ 180
           LK MY + ADV    L+ QIVVLQ ++WY ++LFLFE+   +                  
Sbjct: 121 LKGMYAAAADVDSGSLMVQIVVLQCIIWYTLMLFLFEYRGARLLVMEQFPDTAASIVSFR 180

Query: 181 --------------AAALAPASE------------------------------------- 240
                         AA L   +E                                     
Sbjct: 181 VDSDVVSLAGGGGGAAELQAEAEVGDDGRMRVTVRKSTSSRSEAACSHGTQSHSQSMQPR 240

Query: 241 -----AIEIDTTEE--------------------GLAKEEGEEARDSNGRRHK------- 300
                 +EI + +                     G  K+  EE   + G  H        
Sbjct: 241 VSNLSGVEIYSLQSSRNPTPRGSSFNHAEFFNIVGNGKQGDEEKGAAGGGGHSPQPVVGK 300

Query: 301 ------------------------------------------------------------ 347
                                                                       
Sbjct: 301 RKDLHMFVWSSSASPVSERAAAAAAGAVHVFGGGGADHGDAKGAQAYDEYSFGNKNEKDG 360

BLAST of Csor.00g173140 vs. NCBI nr
Match: KAG6588387.1 (Auxin efflux carrier component 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 675 bits (1742), Expect = 1.71e-244
Identity = 348/348 (100.00%), Postives = 348/348 (100.00%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQVIS 60

Query: 61  DNNLYKMNLQLILADFVQKILAISLMGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           DNNLYKMNLQLILADFVQKILAISLMGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  DNNLYKMNLQLILADFVQKILAISLMGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTKAAALAPASEAIEIDTTEEGLAK 180
           LKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTKAAALAPASEAIEIDTTEEGLAK
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTKAAALAPASEAIEIDTTEEGLAK 180

Query: 181 EEGEEARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLIWASIRFRSNRSIHNNSVNW 240
           EEGEEARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLIWASIRFRSNRSIHNNSVNW
Sbjct: 181 EEGEEARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLIWASIRFRSNRSIHNNSVNW 240

Query: 241 RTWYGHVQLRPFHGFTDKHHSMRYKDDTGSHGNEILNGTCTYGCILTSSRPQGQIAPSGN 300
           RTWYGHVQLRPFHGFTDKHHSMRYKDDTGSHGNEILNGTCTYGCILTSSRPQGQIAPSGN
Sbjct: 241 RTWYGHVQLRPFHGFTDKHHSMRYKDDTGSHGNEILNGTCTYGCILTSSRPQGQIAPSGN 300

Query: 301 RAALPQGIVPFVFAKEYNIHPDVLSTGVLLGLLVALPVALAYYSLLSF 348
           RAALPQGIVPFVFAKEYNIHPDVLSTGVLLGLLVALPVALAYYSLLSF
Sbjct: 301 RAALPQGIVPFVFAKEYNIHPDVLSTGVLLGLLVALPVALAYYSLLSF 348

BLAST of Csor.00g173140 vs. NCBI nr
Match: XP_023529540.1 (auxin efflux carrier component 8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 494 bits (1271), Expect = 1.24e-172
Identity = 288/360 (80.00%), Postives = 298/360 (82.78%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMILAYISV+WWKFFTAEQCCGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMILAYISVKWWKFFTAEQCCGINKFVAKFSIPLLSFQVIS 60

Query: 61  DNNLYKMNLQLILADFVQKILAISLMGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           DNNLYKMNLQLILADFVQKILAI+LMGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  DNNLYKMNLQLILADFVQKILAIALMGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTKAAALAPASEAIEIDTTEEGLAK 180
           LKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTKAAALAPASEAIEIDTTEEGLAK
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTKAAALAPASEAIEIDTTEEGLAK 180

Query: 181 EEGEEARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLIWASIRFR--------SNRS 240
           EEGEEARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLIWASIRFR         +RS
Sbjct: 181 EEGEEARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLIWASIRFRWGVKLPEVIDRS 240

Query: 241 IHNNSVNWRTWYGHVQLRPFHGFTDKHHSMRYKDDTGSHGNEILNGTCTYGCILTSSRPQ 300
           I   S           L  F G      +   K    + G + L G      +  SS P 
Sbjct: 241 ITILSTG-GLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPAL---MAASSLPL 300

Query: 301 GQ----IAPSGNRAALPQGIVPFVFAKEYNIHPDVLSTGVLLGLLVALPVALAYYSLLSF 348
           G     +  +  +AALPQGIVPFVFAKEYNIHPDVLSTGVLLGLLVALPVALAYYSLLSF
Sbjct: 301 GLKGRLLRVAIVQAALPQGIVPFVFAKEYNIHPDVLSTGVLLGLLVALPVALAYYSLLSF 356

BLAST of Csor.00g173140 vs. NCBI nr
Match: XP_022970992.1 (auxin efflux carrier component 8-like [Cucurbita maxima])

HSP 1 Score: 491 bits (1264), Expect = 1.44e-171
Identity = 286/359 (79.67%), Postives = 296/359 (82.45%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQ+IS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQIIS 60

Query: 61  DNNLYKMNLQLILADFVQKILAISLMGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           DNNLYKMNLQLILADFVQKILAI+LMGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  DNNLYKMNLQLILADFVQKILAIALMGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTKAAALAPASEAIEIDTTEEGLAK 180
           LKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTKAAALAPASEAIEID TEEGLAK
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTKAAALAPASEAIEIDITEEGLAK 180

Query: 181 EEGEEARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLIWASIRFR--------SNRS 240
           EEGEEARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLIWASIRFR         +RS
Sbjct: 181 EEGEEARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLIWASIRFRWGVKLPEVIDRS 240

Query: 241 IHNNSVNWRTWYGHVQLRPFHGFTDKHHSMRYKDDTGSHGNEILNGTCTYGCILTSSRPQ 300
           I   S           L  F G      +   K    + G + L G      +  SS P 
Sbjct: 241 ITILSTG-GLGMAMFSLGLFMGSRTSIIACSIKMTLVAMGMKFLMGPAL---MAASSLPL 300

Query: 301 GQ----IAPSGNRAALPQGIVPFVFAKEYNIHPDVLSTGVLLGLLVALPVALAYYSLLS 347
           G     +  +  +AALPQGIVPFVFAKEYNIHPDVLSTGVLLGLLVALPVALAYYSLLS
Sbjct: 301 GLKGRLLRVAIVQAALPQGIVPFVFAKEYNIHPDVLSTGVLLGLLVALPVALAYYSLLS 355

BLAST of Csor.00g173140 vs. NCBI nr
Match: XP_022928686.1 (auxin efflux carrier component 8-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 486 bits (1250), Expect = 2.00e-169
Identity = 287/362 (79.28%), Postives = 295/362 (81.49%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQVIS 60

Query: 61  DNNLYKMNLQLILADFVQKILAISLMGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           DNNLYKMNLQLILADFVQKILAI+LMGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  DNNLYKMNLQLILADFVQKILAIALMGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTKAAALAPASEAIEIDTTEEGLAK 180
           LKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTKAAALAPASEAIEIDTTEEGLAK
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTKAAALAPASEAIEIDTTEEGLAK 180

Query: 181 EEGEEARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLIWASIRFR--------SNRS 240
           EEGEEARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLIWASIRFR         +RS
Sbjct: 181 EEGEEARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLIWASIRFRWGVKLPEVIDRS 240

Query: 241 IHNNSVNWRTWYGHVQLRPFHGFTDKHHSMRYKDDTGSHGNEILNGTCTYGCILTSSRPQ 300
           I   S           L  F G      +   K    + G + L G      +  SS P 
Sbjct: 241 ITILSTG-GLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPAL---MAASSLPL 300

Query: 301 GQIAPSGNRAAL------PQGIVPFVFAKEYNIHPDVLSTGVLLGLLVALPVALAYYSLL 348
           G +     R A+       QGIVPFVFAKEYNIHPDVLSTGVLLGLLVALPVALAYYSLL
Sbjct: 301 G-LKGRLLRVAIVQVCSTSQGIVPFVFAKEYNIHPDVLSTGVLLGLLVALPVALAYYSLL 357

BLAST of Csor.00g173140 vs. NCBI nr
Match: XP_038904643.1 (auxin efflux carrier component 8 [Benincasa hispida])

HSP 1 Score: 451 bits (1161), Expect = 6.59e-156
Identity = 261/355 (73.52%), Postives = 285/355 (80.28%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAM+LAYISV+WWKFFTAEQC GINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  DNNLYKMNLQLILADFVQKILAISLMGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           DNNLYKMNLQLILADF+QKILAI L+GA+TKITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  DNNLYKMNLQLILADFLQKILAIVLLGAITKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTKAAALAPASEAIEIDTTEEGLAK 180
           LKAMYGSEADVLLAQIVVLQS++WYNILLFLFEFTTTKAA+LAPASEA EID T++G AK
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIDITQDGRAK 180

Query: 181 EEGEEARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLIWASIRFRSN---RSIHNNS 240
           EE EEARD NGR  KTRSIL TVARKLI NPNTHATILGLIWASIRFR       + + S
Sbjct: 181 EEIEEARDRNGRSLKTRSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDRS 240

Query: 241 VNWRTWYG----HVQLRPFHGFTDKHHSMRYKDDTGSHGNEILNGTCTYGCILTSSRPQG 300
           +N  +  G       L  F G      +   K    + G + L G         +   +G
Sbjct: 241 INILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGLRG 300

Query: 301 QIAPSGN-RAALPQGIVPFVFAKEYNIHPDVLSTGVLLGLLVALPVALAYYSLLS 347
           ++      +AALPQGIVPFVF+KEYNIHPDVLSTGVLLGLL+ALPVALAYY LLS
Sbjct: 301 RLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLS 355

BLAST of Csor.00g173140 vs. ExPASy TrEMBL
Match: A0A6J1I0Q7 (Auxin efflux carrier component OS=Cucurbita maxima OX=3661 GN=LOC111469793 PE=3 SV=1)

HSP 1 Score: 491 bits (1264), Expect = 6.95e-172
Identity = 286/359 (79.67%), Postives = 296/359 (82.45%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQ+IS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQIIS 60

Query: 61  DNNLYKMNLQLILADFVQKILAISLMGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           DNNLYKMNLQLILADFVQKILAI+LMGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  DNNLYKMNLQLILADFVQKILAIALMGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTKAAALAPASEAIEIDTTEEGLAK 180
           LKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTKAAALAPASEAIEID TEEGLAK
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTKAAALAPASEAIEIDITEEGLAK 180

Query: 181 EEGEEARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLIWASIRFR--------SNRS 240
           EEGEEARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLIWASIRFR         +RS
Sbjct: 181 EEGEEARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLIWASIRFRWGVKLPEVIDRS 240

Query: 241 IHNNSVNWRTWYGHVQLRPFHGFTDKHHSMRYKDDTGSHGNEILNGTCTYGCILTSSRPQ 300
           I   S           L  F G      +   K    + G + L G      +  SS P 
Sbjct: 241 ITILSTG-GLGMAMFSLGLFMGSRTSIIACSIKMTLVAMGMKFLMGPAL---MAASSLPL 300

Query: 301 GQ----IAPSGNRAALPQGIVPFVFAKEYNIHPDVLSTGVLLGLLVALPVALAYYSLLS 347
           G     +  +  +AALPQGIVPFVFAKEYNIHPDVLSTGVLLGLLVALPVALAYYSLLS
Sbjct: 301 GLKGRLLRVAIVQAALPQGIVPFVFAKEYNIHPDVLSTGVLLGLLVALPVALAYYSLLS 355

BLAST of Csor.00g173140 vs. ExPASy TrEMBL
Match: A0A6J1EL02 (Auxin efflux carrier component OS=Cucurbita moschata OX=3662 GN=LOC111435519 PE=3 SV=1)

HSP 1 Score: 486 bits (1250), Expect = 9.70e-170
Identity = 287/362 (79.28%), Postives = 295/362 (81.49%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQVIS 60

Query: 61  DNNLYKMNLQLILADFVQKILAISLMGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           DNNLYKMNLQLILADFVQKILAI+LMGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  DNNLYKMNLQLILADFVQKILAIALMGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTKAAALAPASEAIEIDTTEEGLAK 180
           LKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTKAAALAPASEAIEIDTTEEGLAK
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTKAAALAPASEAIEIDTTEEGLAK 180

Query: 181 EEGEEARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLIWASIRFR--------SNRS 240
           EEGEEARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLIWASIRFR         +RS
Sbjct: 181 EEGEEARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLIWASIRFRWGVKLPEVIDRS 240

Query: 241 IHNNSVNWRTWYGHVQLRPFHGFTDKHHSMRYKDDTGSHGNEILNGTCTYGCILTSSRPQ 300
           I   S           L  F G      +   K    + G + L G      +  SS P 
Sbjct: 241 ITILSTG-GLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPAL---MAASSLPL 300

Query: 301 GQIAPSGNRAAL------PQGIVPFVFAKEYNIHPDVLSTGVLLGLLVALPVALAYYSLL 348
           G +     R A+       QGIVPFVFAKEYNIHPDVLSTGVLLGLLVALPVALAYYSLL
Sbjct: 301 G-LKGRLLRVAIVQVCSTSQGIVPFVFAKEYNIHPDVLSTGVLLGLLVALPVALAYYSLL 357

BLAST of Csor.00g173140 vs. ExPASy TrEMBL
Match: A0A5D3DQ58 (Auxin efflux carrier component OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G008260 PE=3 SV=1)

HSP 1 Score: 447 bits (1150), Expect = 1.50e-154
Identity = 260/356 (73.03%), Postives = 283/356 (79.49%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAM+LAYISV+WWKFFTAEQC GINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  DNNLYKMNLQLILADFVQKILAISLMGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           DNNLYKMNLQLILADF+QKILAI L+GA+TKITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  DNNLYKMNLQLILADFLQKILAIILLGAITKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTKAAALAPASEAIEIDTTEEGLAK 180
           LKAMYGSEADVLLAQIVVLQS++WYNILLFLFEFTTTKAA+LAPASEA EI+ T+EG AK
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITQEGRAK 180

Query: 181 EEGEEARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLIWASIRFR--------SNRS 240
           EE EEARD NGR  KT+SIL TVARKLI NPNTHATILGLIW+SIRFR         +RS
Sbjct: 181 EEIEEARDRNGRSLKTKSILLTVARKLIINPNTHATILGLIWSSIRFRWGVKLPVVIDRS 240

Query: 241 IHNNSVNWRTWYGHVQLRPFHGFTDKHHSMRYKDDTGSHGNEILNGTCTYGCILTSSRPQ 300
           I   S           L  F G      +   K    + G + L G         +   +
Sbjct: 241 ISILSTG-GLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGLR 300

Query: 301 GQIAPSGN-RAALPQGIVPFVFAKEYNIHPDVLSTGVLLGLLVALPVALAYYSLLS 347
           G++      +AALPQGIVPFVF+KEYNIHPDVLSTGVLLGLL+ALPVALAYY LLS
Sbjct: 301 GRLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLS 355

BLAST of Csor.00g173140 vs. ExPASy TrEMBL
Match: A0A1S3B8S2 (Auxin efflux carrier component OS=Cucumis melo OX=3656 GN=LOC103487029 PE=3 SV=1)

HSP 1 Score: 447 bits (1150), Expect = 1.50e-154
Identity = 260/356 (73.03%), Postives = 283/356 (79.49%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAM+LAYISV+WWKFFTAEQC GINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  DNNLYKMNLQLILADFVQKILAISLMGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           DNNLYKMNLQLILADF+QKILAI L+GA+TKITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  DNNLYKMNLQLILADFLQKILAIILLGAITKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTKAAALAPASEAIEIDTTEEGLAK 180
           LKAMYGSEADVLLAQIVVLQS++WYNILLFLFEFTTTKAA+LAPASEA EI+ T+EG AK
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITQEGRAK 180

Query: 181 EEGEEARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLIWASIRFR--------SNRS 240
           EE EEARD NGR  KT+SIL TVARKLI NPNTHATILGLIW+SIRFR         +RS
Sbjct: 181 EEIEEARDRNGRSLKTKSILLTVARKLIINPNTHATILGLIWSSIRFRWGVKLPVVIDRS 240

Query: 241 IHNNSVNWRTWYGHVQLRPFHGFTDKHHSMRYKDDTGSHGNEILNGTCTYGCILTSSRPQ 300
           I   S           L  F G      +   K    + G + L G         +   +
Sbjct: 241 ISILSTG-GLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGLR 300

Query: 301 GQIAPSGN-RAALPQGIVPFVFAKEYNIHPDVLSTGVLLGLLVALPVALAYYSLLS 347
           G++      +AALPQGIVPFVF+KEYNIHPDVLSTGVLLGLL+ALPVALAYY LLS
Sbjct: 301 GRLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLS 355

BLAST of Csor.00g173140 vs. ExPASy TrEMBL
Match: A0A5A7UJS2 (Auxin efflux carrier component OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G001190 PE=3 SV=1)

HSP 1 Score: 442 bits (1138), Expect = 1.03e-152
Identity = 260/357 (72.83%), Postives = 283/357 (79.27%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAM+LAYISV+WWKFFTAEQC GINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  DNNLYKMNLQLILADFVQKILAISLMGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           DNNLYKMNLQLILADF+QKILAI L+GA+TKITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  DNNLYKMNLQLILADFLQKILAIILLGAITKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTKAAALAPASEAI-EIDTTEEGLA 180
           LKAMYGSEADVLLAQIVVLQS++WYNILLFLFEFTTTKAA+LAPASEA  EI+ T+EG A
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATAEIEITQEGRA 180

Query: 181 KEEGEEARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLIWASIRFR--------SNR 240
           KEE EEARD NGR  KT+SIL TVARKLI NPNTHATILGLIW+SIRFR         +R
Sbjct: 181 KEEIEEARDRNGRSLKTKSILLTVARKLIINPNTHATILGLIWSSIRFRWGVKLPVVIDR 240

Query: 241 SIHNNSVNWRTWYGHVQLRPFHGFTDKHHSMRYKDDTGSHGNEILNGTCTYGCILTSSRP 300
           SI   S           L  F G      +   K    + G + L G         +   
Sbjct: 241 SISILSTG-GLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGL 300

Query: 301 QGQIAPSGN-RAALPQGIVPFVFAKEYNIHPDVLSTGVLLGLLVALPVALAYYSLLS 347
           +G++      +AALPQGIVPFVF+KEYNIHPDVLSTGVLLGLL+ALPVALAYY LLS
Sbjct: 301 RGRLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLS 356

BLAST of Csor.00g173140 vs. TAIR 10
Match: AT5G15100.1 (Auxin efflux carrier family protein )

HSP 1 Score: 259.6 bits (662), Expect = 3.6e-69
Identity = 170/370 (45.95%), Postives = 227/370 (61.35%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQVIS 60
           MIS  D+YHVV+ATVPLYV+M L ++S R  K F+ EQC GINKFVAKFSIPLLSFQ+IS
Sbjct: 1   MISWLDIYHVVSATVPLYVSMTLGFLSARHLKLFSPEQCAGINKFVAKFSIPLLSFQIIS 60

Query: 61  DNNLYKMNLQLILADFVQKILAISLMGAVTKI----TSRGG-LNWIITGLSLSTMPNTLI 120
           +NN +KM+ +LIL+D +QK L + ++  V +       RGG L W+ITGLS+S +PNTLI
Sbjct: 61  ENNPFKMSPKLILSDILQKFLVVVVLAMVLRFWHPTGGRGGKLGWVITGLSISVLPNTLI 120

Query: 121 LGLPVLKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTKAAALAPAS------EAI 180
           LG+P+L A+YG EA  +L QIVVLQS++WY ILLFLFE    +A   + AS      +  
Sbjct: 121 LGMPILSAIYGDEAASILEQIVVLQSLIWYTILLFLFELNAARALPSSGASLEHTGNDQE 180

Query: 181 EIDTTEEGLAKEEGEEARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLIWASIRFR- 240
           E +  +E   +E+ EE      R   T  IL    RKLI NPNT+AT++G+IWA++ FR 
Sbjct: 181 EANIEDEPKEEEDEEEVAIVRTRSVGTMKILLKAWRKLIINPNTYATLIGIIWATLHFRL 240

Query: 241 -------SNRSIHNNSVNWRTWYGHVQLRPFHGFTDKHHSMRYKDDTGSHGNEILNG--- 300
                   ++SIH  S +         L  F        +   K    +   + + G   
Sbjct: 241 GWNLPEMIDKSIHLLS-DGGLGMAMFSLGLFMASQSSIIACGTKMAIITMLLKFVLGPAL 300

Query: 301 --TCTYGCILTSSRPQGQIAPSGNRAALPQGIVPFVFAKEYNIHPDVLSTGVLLGLLVAL 347
                Y   L S+  +  I     +AALPQG+VPFVFAKEYN+HP+++STGV+ G+L+AL
Sbjct: 301 MIASAYCIRLKSTLFKVAIL----QAALPQGVVPFVFAKEYNLHPEIISTGVIFGMLIAL 360

BLAST of Csor.00g173140 vs. TAIR 10
Match: AT1G73590.1 (Auxin efflux carrier family protein )

HSP 1 Score: 178.7 bits (452), Expect = 8.0e-45
Identity = 88/158 (55.70%), Postives = 120/158 (75.95%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQVIS 60
           MI+ +D YHV+ A VPLYVAMILAY SV+WWK FT +QC GIN+FVA F++PLLSF  I+
Sbjct: 1   MITAADFYHVMTAMVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIA 60

Query: 61  DNNLYKMNLQLILADFVQKILAISLMGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
            NN Y MNL+ + AD +QK++ +SL+    K++  G L+W IT  SLST+PNTL++G+P+
Sbjct: 61  ANNPYAMNLRFLAADSLQKVIVLSLLFLWCKLSRNGSLDWTITLFSLSTLPNTLVMGIPL 120

Query: 121 LKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTK 159
           LK MYG+ +  L+ QIVVLQ ++WY ++LFLFE+   K
Sbjct: 121 LKGMYGNFSGDLMVQIVVLQCIIWYTLMLFLFEYRGAK 158


HSP 2 Score: 82.8 bits (203), Expect = 6.0e-16
Identity = 59/162 (36.42%), Postives = 88/162 (54.32%), Query Frame = 0

Query: 196 TRSILFTVARKLIYNPNTHATILGLIWASIRFRSN---RSIHNNSVNWRTWYG----HVQ 255
           TR IL  V RKLI NPN+++++ G+ W+ I F+ N    ++   S++  +  G       
Sbjct: 462 TRLILIMVWRKLIRNPNSYSSLFGITWSLISFKWNIEMPALIAKSISILSDAGLGMAMFS 521

Query: 256 LRPFHGFTDKHHSMRYKDDTGSHGNEILNGTCTYGCILTSSRPQG----QIAPSGNRAAL 315
           L  F     +  +   +    +     + G      +L +S   G     +  +  +AAL
Sbjct: 522 LGLFMALNPRIIACGNRRAAFAAAMRFVVGPAV---MLVASYAVGLRGVLLHVAIIQAAL 581

Query: 316 PQGIVPFVFAKEYNIHPDVLSTGVLLGLLVALPVALAYYSLL 347
           PQGIVPFVFAKEYN+HPD+LST V+ G+L+ALP+ L YY LL
Sbjct: 582 PQGIVPFVFAKEYNVHPDILSTAVIFGMLIALPITLLYYILL 620

BLAST of Csor.00g173140 vs. TAIR 10
Match: AT1G70940.1 (Auxin efflux carrier family protein )

HSP 1 Score: 176.0 bits (445), Expect = 5.2e-44
Identity = 92/158 (58.23%), Postives = 115/158 (72.78%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQVIS 60
           MIS  D+Y V+ A +PLYVAMILAY SVRWWK F+ +QC GIN+FVA F++PLLSF  IS
Sbjct: 1   MISWHDLYTVLTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  DNNLYKMNLQLILADFVQKILAISLMGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
            NN Y MNL+ I AD +QKI+ +SL+      T  G L W IT  SLST+PNTL++G+P+
Sbjct: 61  TNNPYAMNLRFIAADTLQKIIMLSLLVLWANFTRSGSLEWSITIFSLSTLPNTLVMGIPL 120

Query: 121 LKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTK 159
           L AMYG  +  L+ QIVVLQ ++WY +LLFLFEF   K
Sbjct: 121 LIAMYGEYSGSLMVQIVVLQCIIWYTLLLFLFEFRGAK 158


HSP 2 Score: 94.4 bits (233), Expect = 2.0e-19
Identity = 73/193 (37.82%), Postives = 98/193 (50.78%), Query Frame = 0

Query: 162 LAPASEAIEIDTTEEGLAKEEGEEARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLI 221
           LAP S A     T  GL   E  + ++       TR IL  V RKLI NPNT+++++GLI
Sbjct: 448 LAPNSTAALQSKT--GLGGAEASQRKNMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLI 507

Query: 222 WASIRFR---SNRSIHNNSVNWRTWYG----HVQLRPFHGFTDKHHSMRYKDDTGSHGNE 281
           WA + FR   +   I   S++  +  G       L  F     K  +      T +    
Sbjct: 508 WALVAFRWHVAMPKIIQQSISILSDAGLGMAMFSLGLFMALQPKLIACGNSVATFAMAVR 567

Query: 282 ILNGTCTYGCILTSSRPQGQIAPSG-NRAALPQGIVPFVFAKEYNIHPDVLSTGVLLGLL 341
            L G         +   +G +      +AALPQGIVPFVFAKEYN+HP +LSTGV+ G+L
Sbjct: 568 FLTGPAVMAVAAIAIGLRGDLLRVAIVQAALPQGIVPFVFAKEYNVHPAILSTGVIFGML 627

Query: 342 VALPVALAYYSLL 347
           +ALP+ L YY LL
Sbjct: 628 IALPITLVYYILL 638

BLAST of Csor.00g173140 vs. TAIR 10
Match: AT5G16530.1 (Auxin efflux carrier family protein )

HSP 1 Score: 172.2 bits (435), Expect = 7.5e-43
Identity = 124/368 (33.70%), Postives = 187/368 (50.82%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQVIS 60
           MI+  DVY V+ A VPLYVA+IL Y SV+WW  FT +QC  IN+ V  F++PL + +  +
Sbjct: 1   MINCGDVYKVIEAMVPLYVALILGYGSVKWWHIFTRDQCDAINRLVCYFTLPLFTIEFTA 60

Query: 61  DNNLYKMNLQLILADFVQKILAISLMGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
             + + MN + I AD + K++ ++++    K +++G   W IT  SL T+ N+L++G+P+
Sbjct: 61  HVDPFNMNYRFIAADVLSKVIIVTVLALWAKYSNKGSYCWSITSFSLCTLTNSLVVGVPL 120

Query: 121 LKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTKAAALAPASEAIEIDTTEEGLAK 180
            KAMYG +A  L+ Q  V Q++VW  +LLF+ EF   KA   +     +++D       K
Sbjct: 121 AKAMYGQQAVDLVVQSSVFQAIVWLTLLLFVLEF--RKAGFSSNNISDVQVDNINIESGK 180

Query: 181 EEGEEARDSNGRRHKTRSILFTVARKLIYNPNTHATILGLIWA----------------S 240
            E     + +        ++  V  KL  NPN ++ ILG+ WA                S
Sbjct: 181 RETVVVGEKS-----FLEVMSLVWLKLATNPNCYSCILGIAWAFISNRWHLELPGILEGS 240

Query: 241 IRFRSNRSIHNNSVNWRTWYGHVQLRPFHGFTDKHHSMRYKDDTG----SHGNEILNGTC 300
           I   S         N   +    +     G +     M  K   G    + G+ +L    
Sbjct: 241 ILIMSKAGTGTAMFNMGIFMALQEKLIVCGTSLTVMGMVLKFIAGPAAMAIGSIVLG--- 300

Query: 301 TYGCILTSSRPQGQIAPSGNRAALPQGIVPFVFAKEYNIHPDVLSTGVLLGLLVALPVAL 349
            +G +L  +  Q         AALPQ I  F+FAKEY +H DVLST V+ G+LV+LPV +
Sbjct: 301 LHGDVLRVAIIQ---------AALPQSITSFIFAKEYGLHADVLSTAVIFGMLVSLPVLV 349

BLAST of Csor.00g173140 vs. TAIR 10
Match: AT1G23080.1 (Auxin efflux carrier family protein )

HSP 1 Score: 171.8 bits (434), Expect = 9.8e-43
Identity = 88/158 (55.70%), Postives = 115/158 (72.78%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMILAYISVRWWKFFTAEQCCGINKFVAKFSIPLLSFQVIS 60
           MI+  D+Y V+ A +PLYVAMILAY SVRWWK F+ +QC GIN+FVA F++PLLSF  IS
Sbjct: 1   MITWHDLYTVLTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  DNNLYKMNLQLILADFVQKILAISLMGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
            NN Y MNL+ I AD +QK++ ++L+      T  G L W IT  SLST+PNTL++G+P+
Sbjct: 61  SNNPYAMNLRFIAADTLQKLIMLTLLIIWANFTRSGSLEWSITIFSLSTLPNTLVMGIPL 120

Query: 121 LKAMYGSEADVLLAQIVVLQSVVWYNILLFLFEFTTTK 159
           L AMYG  +  L+ QIVVLQ ++WY +LLFLFE+   K
Sbjct: 121 LIAMYGEYSGSLMVQIVVLQCIIWYTLLLFLFEYRGAK 158


HSP 2 Score: 93.6 bits (231), Expect = 3.4e-19
Identity = 63/159 (39.62%), Postives = 85/159 (53.46%), Query Frame = 0

Query: 196 TRSILFTVARKLIYNPNTHATILGLIWASIRFR---SNRSIHNNSVNWRTWYG----HVQ 255
           TR IL  V RKLI NPNT+++++GLIWA + FR   +   I   S++  +  G       
Sbjct: 459 TRLILIMVWRKLIRNPNTYSSLIGLIWALVAFRWDVAMPKIIQQSISILSDAGLGMAMFS 518

Query: 256 LRPFHGFTDKHHSMRYKDDTGSHGNEILNGTCTYGCILTSSRPQGQIAPSG-NRAALPQG 315
           L  F     K  +      T +       G         +   +G +      +AALPQG
Sbjct: 519 LGLFMALQPKLIACGNSTATFAMAVRFFTGPAVMAVAAMAIGLRGDLLRVAIVQAALPQG 578

Query: 316 IVPFVFAKEYNIHPDVLSTGVLLGLLVALPVALAYYSLL 347
           IVPFVFAKEYN+HP +LSTGV+ G+L+ALP+ L YY LL
Sbjct: 579 IVPFVFAKEYNVHPAILSTGVIFGMLIALPITLVYYILL 617

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LFP65.0e-6845.95Auxin efflux carrier component 8 OS=Arabidopsis thaliana OX=3702 GN=PIN8 PE=2 SV... [more]
Q0JJV01.2e-5842.45Probable auxin efflux carrier component 8 OS=Oryza sativa subsp. japonica OX=399... [more]
Q5VQY33.5e-4534.03Probable auxin efflux carrier component 9 OS=Oryza sativa subsp. japonica OX=399... [more]
Q5JLM17.8e-4535.14Probable auxin efflux carrier component 5a OS=Oryza sativa subsp. japonica OX=39... [more]
P0C0X53.9e-4428.44Probable auxin efflux carrier component 1b OS=Oryza sativa subsp. japonica OX=39... [more]
Match NameE-valueIdentityDescription
KAG6588387.11.71e-244100.00Auxin efflux carrier component 8, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_023529540.11.24e-17280.00auxin efflux carrier component 8-like [Cucurbita pepo subsp. pepo][more]
XP_022970992.11.44e-17179.67auxin efflux carrier component 8-like [Cucurbita maxima][more]
XP_022928686.12.00e-16979.28auxin efflux carrier component 8-like isoform X2 [Cucurbita moschata][more]
XP_038904643.16.59e-15673.52auxin efflux carrier component 8 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1I0Q76.95e-17279.67Auxin efflux carrier component OS=Cucurbita maxima OX=3661 GN=LOC111469793 PE=3 ... [more]
A0A6J1EL029.70e-17079.28Auxin efflux carrier component OS=Cucurbita moschata OX=3662 GN=LOC111435519 PE=... [more]
A0A5D3DQ581.50e-15473.03Auxin efflux carrier component OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3B8S21.50e-15473.03Auxin efflux carrier component OS=Cucumis melo OX=3656 GN=LOC103487029 PE=3 SV=1[more]
A0A5A7UJS21.03e-15272.83Auxin efflux carrier component OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... [more]
Match NameE-valueIdentityDescription
AT5G15100.13.6e-6945.95Auxin efflux carrier family protein [more]
AT1G73590.18.0e-4555.70Auxin efflux carrier family protein [more]
AT1G70940.15.2e-4458.23Auxin efflux carrier family protein [more]
AT5G16530.17.5e-4333.70Auxin efflux carrier family protein [more]
AT1G23080.19.8e-4355.70Auxin efflux carrier family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004776Membrane transport proteinPFAMPF03547Mem_transcoord: 288..343
e-value: 1.4E-12
score: 46.7
coord: 9..231
e-value: 1.5E-51
score: 175.0
NoneNo IPR availablePANTHERPTHR31752AUXIN EFFLUX CARRIER COMPONENT 1B-RELATEDcoord: 1..234
coord: 302..346
NoneNo IPR availablePANTHERPTHR31752:SF40AUXIN EFFLUX CARRIER COMPONENT 8coord: 302..346
NoneNo IPR availablePANTHERPTHR31752:SF40AUXIN EFFLUX CARRIER COMPONENT 8coord: 1..234

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g173140.m01Csor.00g173140.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010315 auxin efflux
biological_process GO:0010252 auxin homeostasis
biological_process GO:0009926 auxin polar transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0010329 auxin efflux transmembrane transporter activity