Csor.00g172040 (gene) Silver-seed gourd (wild; sororia) v1

BLAST of Csor.00g172040 vs. ExPASy Swiss-Prot
Match: Q9SZY2 (Subtilisin-like protease SBT3.7 OS=Arabidopsis thaliana OX=3702 GN=SBT3.7 PE=3 SV=2)

HSP 1 Score: 607.4 bits (1565), Expect = 2.0e-172
Identity = 357/771 (46.30%), Postives = 464/771 (60.18%), Query Frame = 0

Query: 1   MENNRASSF-MHAVVTIYAVFSAMAEADNQNSKVHIVYLGERPYEDVKLTTDSHHELLES 60
           M N+R S F + ++V I    S         SKVHIVYLGE+ ++D +  T+SHH +L S
Sbjct: 1   MRNHRTSIFVVLSLVIILNGQSGFLPRAGAESKVHIVYLGEKQHDDPEFVTESHHRMLWS 60

Query: 61  VMGSKEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARMSIVARVFPSSLYNMRTTRSWDF 120
           ++GSKE++  SMV+S+RHGFSGFAAKLT SQA+K+A +  V  V P   Y   TTR+WD+
Sbjct: 61  LLGSKEEAHGSMVHSFRHGFSGFAAKLTESQAKKIADLPEVVHVIPDRFYKPATTRTWDY 120

Query: 121 LGLSSSPSASSNLLHRAKMGDNVIIGVIDTG------------MGPIPSRWKGICQSGED 180
           LGL  SP+   NLL++  MG+ +IIG+ID+G            +GP+PS WKG C+SGED
Sbjct: 121 LGL--SPTNPKNLLNQTNMGEQMIIGIIDSGVWPESEVFNDNEIGPVPSHWKGGCESGED 180

Query: 181 FNSSHCNKKVIGARWFARALIADHG--EEAVFKDYLSARDNKGHGTHTASTAGGAFVRNV 240
           FNSSHCNKK+IGA++F  A +A H     +   D++S R   GHGTH A+ AGG++V N 
Sbjct: 181 FNSSHCNKKLIGAKYFINAFLATHESFNSSESLDFISPRGYNGHGTHVATIAGGSYVPNT 240

Query: 241 SYFGNGRGTLRGGAPLARLAIYKVLW---SDSLLGSDADILKGIDEAIHDGVDVLSMSIG 300
           SY G   GT+RGGAP AR+A+YK  W    D    S ADILK +DEAIHDGVDVLS+S+G
Sbjct: 241 SYKGLAGGTVRGGAPRARIAVYKTCWYLDLDIAACSSADILKAMDEAIHDGVDVLSLSLG 300

Query: 301 KSIPLFPVVYDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAATTIDR 360
              PL+P     + +A G+FHA+ KGI+VVCA GN G   QTV N APW+ TVAATT+DR
Sbjct: 301 FE-PLYPETDVRDGIATGAFHAVLKGITVVCAAGNAGPAAQTVGNTAPWILTVAATTLDR 360

Query: 361 AFLASITTLGDNATYLGQTFLKKDIVGKLVVMDRRCAG-------------ILGSDIPIS 420
           +F+  + TLG+N   LGQ       VG   ++     G             ++ S+  ++
Sbjct: 361 SFVTPM-TLGNNKVILGQAIYTGTEVGFTSLVYPENPGNSNESFSGTCERLLINSNRTMA 420

Query: 421 GNVVLLCFIDLAMKAAASNAVMPGKQAKVVGVIYAGQHNDILGS--GDIPSIYVD----- 480
           G VV LCF +     + + A    K+A  +GVI AGQ  ++L     D P + VD     
Sbjct: 421 GKVV-LCFTESPYSISVTRAAHYVKRAGGLGVIIAGQPGNVLRPCLDDFPCVAVDYELGT 480

Query: 481 ----------------------------------------------THPDIAAPGANIIA 540
                                                           PDIAAPG +I+A
Sbjct: 481 YILFYIRSNGSPVVKIQPSRTLIGQPVGTKVASFSSRGPNPISAAILKPDIAAPGVSILA 540

Query: 541 AVPPNHKGGDKGFAAMSGTSMATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDLSE 600
           A   N    D+GF  +SGTSMATP ISGIVAL+K+L P WSPAAI+SA++TTA   D   
Sbjct: 541 ATTTNTTFNDRGFIFLSGTSMATPTISGIVALLKALHPDWSPAAIRSAIVTTAWRTDPFG 600

Query: 601 MPIFAEGSPRKVADPFDYGGGVVDANAAADPGLIYDLGATDYIYYYLCSMGYTEADISHL 660
             IFAEGSPRK ADPFDYGGG+V+   A  PGL+YDLG  DY+  Y+CS+GY E  IS L
Sbjct: 601 EQIFAEGSPRKPADPFDYGGGLVNPEKATKPGLVYDLGLEDYV-LYMCSVGYNETSISQL 660

Query: 661 SQQKTVCPSKRASILDLNLPTITIPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVR 688
             + TVC   + S+LD NLP+ITIP L    T+ RT+TNVG L +VY+  ++ P G++V 
Sbjct: 661 VGKGTVCSYPKPSVLDFNLPSITIPNLKEEVTLPRTLTNVGPLESVYRVAVEPPLGTQVT 720

BLAST of Csor.00g172040 vs. ExPASy Swiss-Prot
Match: Q9ZSB0 (Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana OX=3702 GN=SBT3.9 PE=3 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 1.4e-170
Identity = 351/738 (47.56%), Postives = 456/738 (61.79%), Query Frame = 0

Query: 31  SKVHIVYLGERPYEDVKLTTDSHHELLESVMGSKEKSLESMVYSYRHGFSGFAAKLTNSQ 90
           SKV++VYLGE+ +++ +  T+SHH++L S++GSKE  L+S+VYSYRHGFSGFAAKLT SQ
Sbjct: 27  SKVYVVYLGEKEHDNPESVTESHHQMLWSLLGSKEAVLDSIVYSYRHGFSGFAAKLTESQ 86

Query: 91  AQKLARMSIVARVFPSSLYNMRTTRSWDFLGLSSSPSASSNLLHRAKMGDNVIIGVIDTG 150
           AQ+++ +  V +V P++LY M TTR+WD+LG+  SP  S +LL +A MG NVI+GVID+G
Sbjct: 87  AQQISELPEVVQVIPNTLYEMTTTRTWDYLGV--SPGNSDSLLQKANMGYNVIVGVIDSG 146

Query: 151 M------------GPIPSRWKGICQSGEDFNSS-HCNKKVIGARWFARALIADHG--EEA 210
           +            GPIPSRWKG C+SGE FN+S HCN+K+IGA++F   L+A+ G     
Sbjct: 147 VWPESEMFNDKGFGPIPSRWKGGCESGELFNASIHCNRKLIGAKYFVDGLVAEFGVVNRT 206

Query: 211 VFKDYLSARDNKGHGTHTASTAGGAFVRNVSYFGNGRGTLRGGAPLARLAIYKVLWSDSL 270
              +YLS RD  GHGTH AST GG+F+ NVSY G GRGT RGGAP   +A+YK  WS   
Sbjct: 207 QNPEYLSPRDFAGHGTHVASTIGGSFLPNVSYVGLGRGTARGGAPGVHIAVYKACWSGYC 266

Query: 271 LGSDADILKGIDEAIHDGVDVLSMSIGKSIPLFPVVYDVNPVAVGSFHAIAKGISVVCAG 330
            G  AD+LK +DEAIHDGVD+LS+S+G S+PLFP   +    +VG+FHA+AKGI VV A 
Sbjct: 267 SG--ADVLKAMDEAIHDGVDILSLSLGPSVPLFP---ETEHTSVGAFHAVAKGIPVVIAA 326

Query: 331 GNEGSIQQTVENVAPWLFTVAATTIDRAFLASITTLGDNATYLGQTFLKKDIVGKLVVMD 390
           GN G   QT+ NVAPW+ TVAATT DR+F  +I TLG+N T LGQ       +G      
Sbjct: 327 GNAGPTAQTISNVAPWVLTVAATTQDRSFPTAI-TLGNNITILGQAIYGGPELG------ 386

Query: 391 RRCAGILGSDIPISGNV--------------VLLCFIDLAMKAAASNAVMPGKQAKVVGV 450
               G+   + P+SG+               V+LCF       AA  AV+    A  +G+
Sbjct: 387 --FVGLTYPESPLSGDCEKLSANPNSTMEGKVVLCFAASTPSNAAIAAVI---NAGGLGL 446

Query: 451 IYA-GQHNDILGSGDIPSIYVD-------------------------------------- 510
           I A    + +  +   P + +D                                      
Sbjct: 447 IMAKNPTHSLTPTRKFPWVSIDFELGTDILFYIRSTRSPIVKIQASKTLFGQSVSTKVAT 506

Query: 511 -------------THPDIAAPGANIIAAVPPNHKGGDKGFAAMSGTSMATPHISGIVALI 570
                          PDIAAPG NI+AA+ PN    D GFA MSGTSMATP +SG+V L+
Sbjct: 507 FSSRGPNSVSPAILKPDIAAPGVNILAAISPNSSINDGGFAMMSGTSMATPVVSGVVVLL 566

Query: 571 KSLRPTWSPAAIKSALITTARVEDLSEMPIFAEGSPRKVADPFDYGGGVVDANAAADPGL 630
           KSL P WSP+AIKSA++TTA   D S  PIFA+GS RK+ADPFDYGGG+++   A  PGL
Sbjct: 567 KSLHPDWSPSAIKSAIVTTAWRTDPSGEPIFADGSSRKLADPFDYGGGLINPEKAVKPGL 626

Query: 631 IYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPSKRASILDLNLPTITIPALTNSTTV 688
           IYD+   DY+  Y+CS+ Y++  IS +  + TVCP+ + S+LDLNLP+ITIP L    T+
Sbjct: 627 IYDMTTDDYV-MYMCSVDYSDISISRVLGKITVCPNPKPSVLDLNLPSITIPNLRGEVTL 686

BLAST of Csor.00g172040 vs. ExPASy Swiss-Prot
Match: Q9SZY3 (Subtilisin-like protease SBT3.8 OS=Arabidopsis thaliana OX=3702 GN=SBT3.8 PE=3 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 3.1e-170
Identity = 357/771 (46.30%), Postives = 459/771 (59.53%), Query Frame = 0

Query: 1   MENNRASSFMHAVVTIYAVFSAMAEADNQNSKVHIVYLGERPYEDVKLTTDSHHELLESV 60
           M++ R   F+  ++   + F A A A+   SKVHIVYLGE+ ++D +  T+SHH +L S+
Sbjct: 1   MKSCRTLIFVAIILNGLSTFVAHAGAE---SKVHIVYLGEKQHDDPEFVTESHHRMLWSL 60

Query: 61  MGSKEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARMSIVARVFPSSLYNMRTTRSWDFL 120
           +GSKE +  SMV+SYRHGFSGFAAKLT SQA+KLA +  V  V P S Y + TTR+WD+L
Sbjct: 61  LGSKEDAHSSMVHSYRHGFSGFAAKLTKSQAKKLADLPEVVHVTPDSFYQLDTTRTWDYL 120

Query: 121 GLSSSPSASSNLLHRAKMGDNVIIGVIDT------------GMGPIPSRWKGICQSGEDF 180
           GL  S +   NLL+   MG+ VIIG++D+            G+GP+PS WKG C SGE+F
Sbjct: 121 GL--SVANPKNLLNDTNMGEEVIIGIVDSGVWPESEVFNDNGIGPVPSHWKGGCVSGENF 180

Query: 181 NSSHCNKKVIGARWFARALIADHG--EEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVS 240
            SS CNKK+IGA++F    +A H         D++S RD  GHGTH A+ AGG++V ++S
Sbjct: 181 TSSQCNKKLIGAKYFINGFLATHESFNSTESLDFISPRDRSGHGTHVATIAGGSYVPSIS 240

Query: 241 YFGNGRGTLRGGAPLARLAIYKVLW----SDSLLGSDADILKGIDEAIHDGVDVLSMSIG 300
           Y G   GT+RGGAP AR+A+YK  W     D    S ADILK +DEA+HDGVDVLS+SIG
Sbjct: 241 YKGLAGGTVRGGAPRARIAMYKACWYLDRFDINTCSSADILKAMDEAMHDGVDVLSLSIG 300

Query: 301 KSIPLFPVVYDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAATTIDR 360
              P FP       +A G+FHA+ KGI+VVC+GGN G   QTV N APW+ TVAATT+DR
Sbjct: 301 YRFPYFPETDVRAVIATGAFHAVLKGITVVCSGGNSGPAAQTVGNTAPWILTVAATTLDR 360

Query: 361 AFLASITTLGDNATYLGQTFLKKDIVGKLVVMDRRCAG-------------ILGSDIPIS 420
           +F   I TLG+N   LGQ       +G   ++     G                S+  ++
Sbjct: 361 SFPTPI-TLGNNKLILGQAMYTGPELGFTSLVYPENPGNSNESFSGDCELLFFNSNHTMA 420

Query: 421 GNVVLLCFIDLAMKAAASNAVMPGKQAKVVGVIYAGQHNDILG--SGDIPSIYVD----- 480
           G VV LCF         S+AV   K+A  +GVI A    D L     D P + VD     
Sbjct: 421 GKVV-LCFTTSTRYITVSSAVSYVKEAGGLGVIVARNPGDNLSPCEDDFPCVAVDYELGT 480

Query: 481 ----------------------------------------------THPDIAAPGANIIA 540
                                                           PDIAAPG +I+A
Sbjct: 481 DILLYIRSTGLPVVKIQPSKTLVGQPVGTKVADFSSRGPNSIEPAILKPDIAAPGVSILA 540

Query: 541 AVPPNHKGGDKGFAAMSGTSMATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDLSE 600
           A   N    D+GF  +SGTSMA P ISG+VAL+K+L   WSPAAI+SA++TTA   D   
Sbjct: 541 ATTTNKTFNDRGFIFLSGTSMAAPTISGVVALLKALHRDWSPAAIRSAIVTTAWRTDPFG 600

Query: 601 MPIFAEGSPRKVADPFDYGGGVVDANAAADPGLIYDLGATDYIYYYLCSMGYTEADISHL 660
             IFAEGSPRK+ADPFDYGGG+V+   AA PGL+YDLG  DY+  Y+CS+GY E  IS L
Sbjct: 601 EQIFAEGSPRKLADPFDYGGGLVNPEKAAKPGLVYDLGLEDYV-LYMCSVGYNETSISQL 660

Query: 661 SQQKTVCPSKRASILDLNLPTITIPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVR 688
             + TVC + + S+LD NLP+ITIP L +  T+TRT+TNVG L +VYK VI+ P G +V 
Sbjct: 661 VGKGTVCSNPKPSVLDFNLPSITIPNLKDEVTLTRTLTNVGQLESVYKVVIEPPIGIQVT 720

BLAST of Csor.00g172040 vs. ExPASy Swiss-Prot
Match: Q9MAP5 (Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 5.9e-169
Identity = 358/765 (46.80%), Postives = 462/765 (60.39%), Query Frame = 0

Query: 7   SSFMHAVVTIYAVFSAMAEADNQNSKVHIVYLGERPYEDVKLTTDSHHELLESVMGSKEK 66
           SS +  ++++  V +A        SKVHIVYLGE+ + D +  T+SHH++L S++GSK+ 
Sbjct: 6   SSILLVLLSLITVLNATRARSETESKVHIVYLGEKKHHDPEFVTESHHQMLASLLGSKKD 65

Query: 67  SLESMVYSYRHGFSGFAAKLTNSQAQKLARMSIVARVFPSSLYNMRTTRSWDFLGLSSSP 126
           + +SMVYSYRHGFSGFAAKLT SQA+K+A +  V  V P   + + TTR+W++LGLSS  
Sbjct: 66  ADDSMVYSYRHGFSGFAAKLTKSQAKKIADLPEVVHVIPDGFHELATTRTWEYLGLSS-- 125

Query: 127 SASSNLLHRAKMGDNVIIGVIDT------------GMGPIPSRWKGICQSGEDFNSSHCN 186
           +   NLL+   MGD VIIGVIDT            G+GPIP +WKG C+SGE+F S+ CN
Sbjct: 126 ANPKNLLNDTNMGDQVIIGVIDTGVWPESESFNDNGVGPIPRKWKGGCESGENFRSTDCN 185

Query: 187 KKVIGARWFARALIADHG--EEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYFGNGR 246
           +K+IGA++F    +A++        +DY+SARD  GHGTH AS AGG+FV NVSY G   
Sbjct: 186 RKLIGAKYFINGFLAENKGFNTTESRDYISARDFDGHGTHVASIAGGSFVPNVSYKGLAG 245

Query: 247 GTLRGGAPLARLAIYKVLW-SDSLLG---SDADILKGIDEAIHDGVDVLSMSIGKSIPLF 306
           GTLRGGAP AR+A+YK  W  + L G   SD+DI+K IDEAIHDGVDVLS+S+   IPL 
Sbjct: 246 GTLRGGAPRARIAMYKACWFHEELKGVTCSDSDIMKAIDEAIHDGVDVLSISLVGQIPLN 305

Query: 307 PVVYDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAATTIDRAFLASI 366
                 +  A G FHA+AKGI VVCAGGN+G   QTV N+APW+ TVAATT+DR+F   I
Sbjct: 306 SETDIRDEFATGLFHAVAKGIVVVCAGGNDGPAAQTVVNIAPWILTVAATTLDRSFPTPI 365

Query: 367 TTLGDNATYLGQTFLKKDIVGKLVVM--------DRRCAGILGSDIPISGNV-----VLL 426
            TLG+N   LGQ       +G   ++        +   +G+  S + ++ N      V+L
Sbjct: 366 -TLGNNKVILGQATYTGPELGLTSLVYPENARNNNETFSGVCES-LNLNPNYTMAMKVVL 425

Query: 427 CFIDLAMKAAASNAVMPGKQAKVVGVIYAGQHNDILG--SGDIPSIYVD----------- 486
           CF      AA S A    K A  +G+I +      L   + D P + VD           
Sbjct: 426 CFTASRTNAAISRAASFVKAAGGLGLIISRNPVYTLSPCNDDFPCVAVDYELGTDILSYI 485

Query: 487 ----------------------------------------THPDIAAPGANIIAAVPPNH 546
                                                     PDIAAPG  I+AA  PN 
Sbjct: 486 RSTRSPVVKIQRSRTLSGQPVGTKVVNFSSRGPNSMSPAILKPDIAAPGVRILAATSPND 545

Query: 547 KGGDKGFAAMSGTSMATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDLSEMPIFAE 606
                GFA +SGTSMATP ISG++AL+K+L P WSPAA +SA++TTA   D     IFAE
Sbjct: 546 TLNVGGFAMLSGTSMATPVISGVIALLKALHPEWSPAAFRSAIVTTAWRTDPFGEQIFAE 605

Query: 607 GSPRKVADPFDYGGGVVDANAAADPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTV 666
           GS RKV+DPFDYGGG+V+   AA+PGLIYD+G  DYI  YLCS GY ++ IS L  Q TV
Sbjct: 606 GSSRKVSDPFDYGGGIVNPEKAAEPGLIYDMGPQDYI-LYLCSAGYNDSSISQLVGQITV 665

Query: 667 CPSKRASILDLNLPTITIPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVL 688
           C + + S+LD+NLP+ITIP L +  T+TRTVTNVG + +VYK  ++ P G +V V P  L
Sbjct: 666 CSNPKPSVLDVNLPSITIPNLKDEVTLTRTVTNVGLVDSVYKVSVEPPLGVRVVVTPETL 725

BLAST of Csor.00g172040 vs. ExPASy Swiss-Prot
Match: Q9MAP7 (Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 2.9e-168
Identity = 351/759 (46.25%), Postives = 455/759 (59.95%), Query Frame = 0

Query: 13  VVTIYAVFSAMAEADNQNSKVHIVYLGERPYEDVKLTTDSHHELLESVMGSKEKSLESMV 72
           V+++  V + +  +D   SKVHIVYLGE+ ++D +  ++SHH++L S++GSK  + ESMV
Sbjct: 11  VLSLVIVLNVVRASD--ESKVHIVYLGEKQHDDPEFVSESHHQMLSSLLGSKVDAHESMV 70

Query: 73  YSYRHGFSGFAAKLTNSQAQKLARMSIVARVFPSSLYNMRTTRSWDFLGLSSSPSASSNL 132
           YSYRHGFSGFAAKLT SQA+KLA    V  V   S Y + TTR+WD+LGL  S +  +NL
Sbjct: 71  YSYRHGFSGFAAKLTESQAKKLADSPEVVHVMADSFYELATTRTWDYLGL--SVANPNNL 130

Query: 133 LHRAKMGDNVIIGVIDT------------GMGPIPSRWKGICQSGEDFNSSHCNKKVIGA 192
           L+   MGD VIIG IDT            G+GPIPS WKG C+SGE F S++CN+K+IGA
Sbjct: 131 LNDTNMGDQVIIGFIDTGVWPESESFNDNGVGPIPSHWKGGCESGEKFISTNCNRKLIGA 190

Query: 193 RWFARALIADHG--EEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYFGNGRGTLRGG 252
           ++F    +A++        +DY+SARD  GHGTHTAS AGG+FV N+SY G   G LRGG
Sbjct: 191 KYFINGFLAENEGFNTTESRDYISARDFIGHGTHTASIAGGSFVPNISYKGLAGGNLRGG 250

Query: 253 APLARLAIYKVLWSDSLLG----SDADILKGIDEAIHDGVDVLSMSIGKSIPLFPVVYDV 312
           AP AR+AIYK  W    LG    S +DILK +DE++HDGVDVLS+S+G  IPL+P     
Sbjct: 251 APRARIAIYKACWYVDQLGAVACSSSDILKAMDESMHDGVDVLSLSLGAQIPLYPETDLR 310

Query: 313 NPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAATTIDRAFLASITTLGDN 372
           + +A G+FHA+AKGI VVCAGGN G   QTV N APW+ TVAATT+DR+F   I TLG+ 
Sbjct: 311 DRIATGAFHAVAKGIIVVCAGGNSGPAAQTVLNTAPWIITVAATTLDRSFPTPI-TLGNR 370

Query: 373 ATYLGQTFLKKDIVGKLVVMDRRCAGI-------------LGSDIPISGNVVLLCFIDLA 432
              LGQ       +G   ++    AG              L  +  ++G VV LCF    
Sbjct: 371 KVILGQALYTGQELGFTSLVYPENAGFTNETFSGVCERLNLNPNRTMAGKVV-LCFTTNT 430

Query: 433 MKAAASNAVMPGKQAKVVGVIYAGQ--HNDILGSGDIPSIYVD----------------- 492
           +  A S A    K A  +GVI A    +N      D P + +D                 
Sbjct: 431 LFTAVSRAASYVKAAGGLGVIIARNPGYNLTPCRDDFPCVAIDYELGTDVLLYIRSTRSP 490

Query: 493 ----------------------------------THPDIAAPGANIIAAVPPNHKGGDKG 552
                                               PDI APG +I+AA  P+      G
Sbjct: 491 VVKIQPSRTLVGQPVGTKVATFSSRGPNSISPAILKPDIGAPGVSILAATSPDSNSSVGG 550

Query: 553 FAAMSGTSMATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDLSEMPIFAEGSPRKV 612
           F  ++GTSMA P ++G+VAL+K+L P WSPAA +SA++TTA   D     IFAEGS RKV
Sbjct: 551 FDILAGTSMAAPVVAGVVALLKALHPNWSPAAFRSAIVTTAWRTDPFGEQIFAEGSSRKV 610

Query: 613 ADPFDYGGGVVDANAAADPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPSKRA 672
           ADPFDYGGG+V+   AADPGLIYD+G  DYI  YLCS GY ++ I+ L    TVC + + 
Sbjct: 611 ADPFDYGGGIVNPEKAADPGLIYDMGPRDYI-LYLCSAGYNDSSITQLVGNVTVCSTPKT 670

Query: 673 SILDLNLPTITIPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNV 688
           S+LD+NLP+ITIP L +  T+TRTVTNVG + +VYK V++ P G +V V P  LVFNS  
Sbjct: 671 SVLDVNLPSITIPDLKDEVTLTRTVTNVGTVDSVYKVVVEPPLGIQVVVAPETLVFNSKT 730

BLAST of Csor.00g172040 vs. NCBI nr
Match: KAG6577197.1 (Subtilisin-like protease 3.7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1360 bits (3519), Expect = 0.0
Identity = 692/692 (100.00%), Postives = 692/692 (100.00%), Query Frame = 0

Query: 1   MENNRASSFMHAVVTIYAVFSAMAEADNQNSKVHIVYLGERPYEDVKLTTDSHHELLESV 60
           MENNRASSFMHAVVTIYAVFSAMAEADNQNSKVHIVYLGERPYEDVKLTTDSHHELLESV
Sbjct: 1   MENNRASSFMHAVVTIYAVFSAMAEADNQNSKVHIVYLGERPYEDVKLTTDSHHELLESV 60

Query: 61  MGSKEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARMSIVARVFPSSLYNMRTTRSWDFL 120
           MGSKEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARMSIVARVFPSSLYNMRTTRSWDFL
Sbjct: 61  MGSKEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARMSIVARVFPSSLYNMRTTRSWDFL 120

Query: 121 GLSSSPSASSNLLHRAKMGDNVIIGVIDTGMGPIPSRWKGICQSGEDFNSSHCNKKVIGA 180
           GLSSSPSASSNLLHRAKMGDNVIIGVIDTGMGPIPSRWKGICQSGEDFNSSHCNKKVIGA
Sbjct: 121 GLSSSPSASSNLLHRAKMGDNVIIGVIDTGMGPIPSRWKGICQSGEDFNSSHCNKKVIGA 180

Query: 181 RWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYFGNGRGTLRGGAP 240
           RWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYFGNGRGTLRGGAP
Sbjct: 181 RWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYFGNGRGTLRGGAP 240

Query: 241 LARLAIYKVLWSDSLLGSDADILKGIDEAIHDGVDVLSMSIGKSIPLFPVVYDVNPVAVG 300
           LARLAIYKVLWSDSLLGSDADILKGIDEAIHDGVDVLSMSIGKSIPLFPVVYDVNPVAVG
Sbjct: 241 LARLAIYKVLWSDSLLGSDADILKGIDEAIHDGVDVLSMSIGKSIPLFPVVYDVNPVAVG 300

Query: 301 SFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAATTIDRAFLASITTLGDNATYLGQ 360
           SFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAATTIDRAFLASITTLGDNATYLGQ
Sbjct: 301 SFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAATTIDRAFLASITTLGDNATYLGQ 360

Query: 361 TFLKKDIVGKLVVMDRRCAGILGSDIPISGNVVLLCFIDLAMKAAASNAVMPGKQAKVVG 420
           TFLKKDIVGKLVVMDRRCAGILGSDIPISGNVVLLCFIDLAMKAAASNAVMPGKQAKVVG
Sbjct: 361 TFLKKDIVGKLVVMDRRCAGILGSDIPISGNVVLLCFIDLAMKAAASNAVMPGKQAKVVG 420

Query: 421 VIYAGQHNDILGSGDIPSIYVDTHPDIAAPGANIIAAVPPNHKGGDKGFAAMSGTSMATP 480
           VIYAGQHNDILGSGDIPSIYVDTHPDIAAPGANIIAAVPPNHKGGDKGFAAMSGTSMATP
Sbjct: 421 VIYAGQHNDILGSGDIPSIYVDTHPDIAAPGANIIAAVPPNHKGGDKGFAAMSGTSMATP 480

Query: 481 HISGIVALIKSLRPTWSPAAIKSALITTARVEDLSEMPIFAEGSPRKVADPFDYGGGVVD 540
           HISGIVALIKSLRPTWSPAAIKSALITTARVEDLSEMPIFAEGSPRKVADPFDYGGGVVD
Sbjct: 481 HISGIVALIKSLRPTWSPAAIKSALITTARVEDLSEMPIFAEGSPRKVADPFDYGGGVVD 540

Query: 541 ANAAADPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPSKRASILDLNLPTITI 600
           ANAAADPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPSKRASILDLNLPTITI
Sbjct: 541 ANAAADPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPSKRASILDLNLPTITI 600

Query: 601 PALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKKISFKVKFSST 660
           PALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKKISFKVKFSST
Sbjct: 601 PALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKKISFKVKFSST 660

Query: 661 LQRNYGYSFGRLTWTDGVHLVKSPLSVGFDFF 692
           LQRNYGYSFGRLTWTDGVHLVKSPLSVGFDFF
Sbjct: 661 LQRNYGYSFGRLTWTDGVHLVKSPLSVGFDFF 692

BLAST of Csor.00g172040 vs. NCBI nr
Match: XP_022931493.1 (subtilisin-like protease SBT3.9 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1300 bits (3364), Expect = 0.0
Identity = 681/762 (89.37%), Postives = 686/762 (90.03%), Query Frame = 0

Query: 1   MENNRASSFMHAVVTIYAVFSAMAEADNQNSKVHIVYLGERPYEDVKLTTDSHHELLESV 60
           MENNRASSFMHAVVTIYAVFSAMAEADNQNSKV+I YLGERPYEDVKLTTDSHHELLESV
Sbjct: 1   MENNRASSFMHAVVTIYAVFSAMAEADNQNSKVYIAYLGERPYEDVKLTTDSHHELLESV 60

Query: 61  MGSKEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARMSIVARVFPSSLYNMRTTRSWDFL 120
           MGSKEKSLESM+YSYRHGFSGFAAKLTNSQAQKLARM IVARVFPSSLY MRTTRSWDFL
Sbjct: 61  MGSKEKSLESMMYSYRHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMRTTRSWDFL 120

Query: 121 GLSSSPSASSNLLHRAKMGDNVIIGVIDTG------------MGPIPSRWKGICQSGEDF 180
           GLSSSPSASSNLLHRAKMGDNVIIGVIDTG            MGPIPSRWKGICQSGEDF
Sbjct: 121 GLSSSPSASSNLLHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGICQSGEDF 180

Query: 181 NSSHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYF 240
           NSSHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYF
Sbjct: 181 NSSHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYF 240

Query: 241 GNGRGTLRGGAPLARLAIYKVLWSDSLLGSDADILKGIDEAIHDGVDVLSMSIGKSIPLF 300
           GNGRGTLRGGAPLARLAIYKVLWSDSLLGSDADILKGIDEAIHDGVDVLSMSIGKSIPLF
Sbjct: 241 GNGRGTLRGGAPLARLAIYKVLWSDSLLGSDADILKGIDEAIHDGVDVLSMSIGKSIPLF 300

Query: 301 PVVYDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAATTIDRAFLASI 360
           PVVYDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAA+TIDRAFLASI
Sbjct: 301 PVVYDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAASTIDRAFLASI 360

Query: 361 TTLGDNATYLGQTFLKKDIVGKLVVMDRRCAGILGSDIPISGNVVLLCFIDLAMKAAASN 420
           TTLGDNATYLGQTFLKKD+VGKLVVMDRRCAGILGSDIPISGNVVLLCFIDLAMKA ASN
Sbjct: 361 TTLGDNATYLGQTFLKKDVVGKLVVMDRRCAGILGSDIPISGNVVLLCFIDLAMKAGASN 420

Query: 421 AVMPGKQAKVVGVIYAGQHNDILGSGDIPSIYVDTH------------------------ 480
           AVMPGKQAKVVGVIYAGQHNDILGSGDIPSIYVDTH                        
Sbjct: 421 AVMPGKQAKVVGVIYAGQHNDILGSGDIPSIYVDTHVGTKLFNYLITDDHALIRLTATQT 480

Query: 481 ---------------------------PDIAAPGANIIAAVPPNHKGGDKGFAAMSGTSM 540
                                      PDIAAPG+NIIAAVPPNHKGGDKGFAAMSGTSM
Sbjct: 481 IIGKPISSRIAFFSSRGPNSVSPGILKPDIAAPGSNIIAAVPPNHKGGDKGFAAMSGTSM 540

Query: 541 ATPHIS-------GIVALIKSLRPTWSPAAIKSALITTARVEDLSEMPIFAEGSPRKVAD 600
           ATPHIS       GIVALIKSLRPTWSPAAIKSALITTARVEDLSEMPIFAEGSPRKVAD
Sbjct: 541 ATPHISMATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDLSEMPIFAEGSPRKVAD 600

Query: 601 PFDYGGGVVDANAAADPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPSKRASI 660
           PFDYGGGVVDANAAADPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVC SKRASI
Sbjct: 601 PFDYGGGVVDANAAADPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCSSKRASI 660

Query: 661 LDLNLPTITIPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKK 692
           LDLNLPTITIPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKK
Sbjct: 661 LDLNLPTITIPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKK 720

BLAST of Csor.00g172040 vs. NCBI nr
Match: XP_022931494.1 (subtilisin-like protease SBT3.9 isoform X2 [Cucurbita moschata])

HSP 1 Score: 1288 bits (3333), Expect = 0.0
Identity = 677/762 (88.85%), Postives = 683/762 (89.63%), Query Frame = 0

Query: 1   MENNRASSFMHAVVTIYAVFSAMAEADNQNSKVHIVYLGERPYEDVKLTTDSHHELLESV 60
           MENNRASSFMHAVVTIYAVFSAMAEADNQNSKV+I YLGERPYEDVKLTTDSHHELLESV
Sbjct: 1   MENNRASSFMHAVVTIYAVFSAMAEADNQNSKVYIAYLGERPYEDVKLTTDSHHELLESV 60

Query: 61  MGSKEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARMSIVARVFPSSLYNMRTTRSWDFL 120
           MGSKEKSLESM+YSYRHGFSGFAAKLTNSQAQKLA    +ARVFPSSLY MRTTRSWDFL
Sbjct: 61  MGSKEKSLESMMYSYRHGFSGFAAKLTNSQAQKLA----LARVFPSSLYKMRTTRSWDFL 120

Query: 121 GLSSSPSASSNLLHRAKMGDNVIIGVIDTG------------MGPIPSRWKGICQSGEDF 180
           GLSSSPSASSNLLHRAKMGDNVIIGVIDTG            MGPIPSRWKGICQSGEDF
Sbjct: 121 GLSSSPSASSNLLHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGICQSGEDF 180

Query: 181 NSSHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYF 240
           NSSHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYF
Sbjct: 181 NSSHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYF 240

Query: 241 GNGRGTLRGGAPLARLAIYKVLWSDSLLGSDADILKGIDEAIHDGVDVLSMSIGKSIPLF 300
           GNGRGTLRGGAPLARLAIYKVLWSDSLLGSDADILKGIDEAIHDGVDVLSMSIGKSIPLF
Sbjct: 241 GNGRGTLRGGAPLARLAIYKVLWSDSLLGSDADILKGIDEAIHDGVDVLSMSIGKSIPLF 300

Query: 301 PVVYDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAATTIDRAFLASI 360
           PVVYDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAA+TIDRAFLASI
Sbjct: 301 PVVYDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAASTIDRAFLASI 360

Query: 361 TTLGDNATYLGQTFLKKDIVGKLVVMDRRCAGILGSDIPISGNVVLLCFIDLAMKAAASN 420
           TTLGDNATYLGQTFLKKD+VGKLVVMDRRCAGILGSDIPISGNVVLLCFIDLAMKA ASN
Sbjct: 361 TTLGDNATYLGQTFLKKDVVGKLVVMDRRCAGILGSDIPISGNVVLLCFIDLAMKAGASN 420

Query: 421 AVMPGKQAKVVGVIYAGQHNDILGSGDIPSIYVDTH------------------------ 480
           AVMPGKQAKVVGVIYAGQHNDILGSGDIPSIYVDTH                        
Sbjct: 421 AVMPGKQAKVVGVIYAGQHNDILGSGDIPSIYVDTHVGTKLFNYLITDDHALIRLTATQT 480

Query: 481 ---------------------------PDIAAPGANIIAAVPPNHKGGDKGFAAMSGTSM 540
                                      PDIAAPG+NIIAAVPPNHKGGDKGFAAMSGTSM
Sbjct: 481 IIGKPISSRIAFFSSRGPNSVSPGILKPDIAAPGSNIIAAVPPNHKGGDKGFAAMSGTSM 540

Query: 541 ATPHIS-------GIVALIKSLRPTWSPAAIKSALITTARVEDLSEMPIFAEGSPRKVAD 600
           ATPHIS       GIVALIKSLRPTWSPAAIKSALITTARVEDLSEMPIFAEGSPRKVAD
Sbjct: 541 ATPHISMATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDLSEMPIFAEGSPRKVAD 600

Query: 601 PFDYGGGVVDANAAADPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPSKRASI 660
           PFDYGGGVVDANAAADPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVC SKRASI
Sbjct: 601 PFDYGGGVVDANAAADPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCSSKRASI 660

Query: 661 LDLNLPTITIPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKK 692
           LDLNLPTITIPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKK
Sbjct: 661 LDLNLPTITIPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKK 720

BLAST of Csor.00g172040 vs. NCBI nr
Match: XP_022931499.1 (subtilisin-like protease SBT3.9 isoform X7 [Cucurbita moschata])

HSP 1 Score: 1262 bits (3266), Expect = 0.0
Identity = 661/744 (88.84%), Postives = 668/744 (89.78%), Query Frame = 0

Query: 19  VFSAMAEADNQNSKVHIVYLGERPYEDVKLTTDSHHELLESVMGSKEKSLESMVYSYRHG 78
           +FSAMAEAD+QNSKV+I YLGERPYEDVKLTTDSHHELLESVMGSKEKSLESM+YSYRHG
Sbjct: 1   MFSAMAEADDQNSKVYIAYLGERPYEDVKLTTDSHHELLESVMGSKEKSLESMMYSYRHG 60

Query: 79  FSGFAAKLTNSQAQKLARMSIVARVFPSSLYNMRTTRSWDFLGLSSSPSASSNLLHRAKM 138
           FSGFAAKLTNSQAQKLARM IVARVFPSSLY MRTTRSWDFLGLSSSPSASSNLLHRAKM
Sbjct: 61  FSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMRTTRSWDFLGLSSSPSASSNLLHRAKM 120

Query: 139 GDNVIIGVIDTG------------MGPIPSRWKGICQSGEDFNSSHCNKKVIGARWFARA 198
           GDNVIIGVIDTG            MGPIPSRWKGICQSGEDFNSSHCNKKVIGARWFARA
Sbjct: 121 GDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGICQSGEDFNSSHCNKKVIGARWFARA 180

Query: 199 LIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYFGNGRGTLRGGAPLARLAI 258
           LIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYFGNGRGTLRGGAPLARLAI
Sbjct: 181 LIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYFGNGRGTLRGGAPLARLAI 240

Query: 259 YKVLWSDSLLGSDADILKGIDEAIHDGVDVLSMSIGKSIPLFPVVYDVNPVAVGSFHAIA 318
           YKVLWSDSLLGSDADILKGIDEAIHDGVDVLSMSIGKSIPLFPVVYDVNPVAVGSFHAIA
Sbjct: 241 YKVLWSDSLLGSDADILKGIDEAIHDGVDVLSMSIGKSIPLFPVVYDVNPVAVGSFHAIA 300

Query: 319 KGISVVCAGGNEGSIQQTVENVAPWLFTVAATTIDRAFLASITTLGDNATYLGQTFLKKD 378
           KGISVVCAGGNEGSIQQTVENVAPWLFTVAA+TIDRAFLASITTLGDNATYLGQTFLKKD
Sbjct: 301 KGISVVCAGGNEGSIQQTVENVAPWLFTVAASTIDRAFLASITTLGDNATYLGQTFLKKD 360

Query: 379 IVGKLVVMDRRCAGILGSDIPISGNVVLLCFIDLAMKAAASNAVMPGKQAKVVGVIYAGQ 438
           +VGKLVVMDRRCAGILGSDIPISGNVVLLCFIDLAMKA ASNAVMPGKQAKVVGVIYAGQ
Sbjct: 361 VVGKLVVMDRRCAGILGSDIPISGNVVLLCFIDLAMKAGASNAVMPGKQAKVVGVIYAGQ 420

Query: 439 HNDILGSGDIPSIYVDTH------------------------------------------ 498
           HNDILGSGDIPSIYVDTH                                          
Sbjct: 421 HNDILGSGDIPSIYVDTHVGTKLFNYLITDDHALIRLTATQTIIGKPISSRIAFFSSRGP 480

Query: 499 ---------PDIAAPGANIIAAVPPNHKGGDKGFAAMSGTSMATPHIS-------GIVAL 558
                    PDIAAPG+NIIAAVPPNHKGGDKGFAAMSGTSMATPHIS       GIVAL
Sbjct: 481 NSVSPGILKPDIAAPGSNIIAAVPPNHKGGDKGFAAMSGTSMATPHISMATPHISGIVAL 540

Query: 559 IKSLRPTWSPAAIKSALITTARVEDLSEMPIFAEGSPRKVADPFDYGGGVVDANAAADPG 618
           IKSLRPTWSPAAIKSALITTARVEDLSEMPIFAEGSPRKVADPFDYGGGVVDANAAADPG
Sbjct: 541 IKSLRPTWSPAAIKSALITTARVEDLSEMPIFAEGSPRKVADPFDYGGGVVDANAAADPG 600

Query: 619 LIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPSKRASILDLNLPTITIPALTNSTT 678
           LIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVC SKRASILDLNLPTITIPALTNSTT
Sbjct: 601 LIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCSSKRASILDLNLPTITIPALTNSTT 660

Query: 679 VTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKKISFKVKFSSTLQRNYGYS 692
           VTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKKISFKVKFSSTLQRNYGYS
Sbjct: 661 VTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKKISFKVKFSSTLQRNYGYS 720

BLAST of Csor.00g172040 vs. NCBI nr
Match: KAG6577196.1 (Subtilisin-like protease 3.7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1240 bits (3208), Expect = 0.0
Identity = 635/694 (91.50%), Postives = 655/694 (94.38%), Query Frame = 0

Query: 1   MENNRASSFMHAV--VTIYAVFSAMAEADNQNSKVHIVYLGERPYEDVKLTTDSHHELLE 60
           MENN+A SFM AV  VTIYAVFSAMAE D+QNSKVHIVYLGERPY+DVKLTT SHHELLE
Sbjct: 1   MENNQAPSFMLAVAIVTIYAVFSAMAEVDDQNSKVHIVYLGERPYDDVKLTTHSHHELLE 60

Query: 61  SVMGSKEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARMSIVARVFPSSLYNMRTTRSWD 120
           SV+GSKEKSLESMVYSY+HGFSGFAAKLTNSQAQKLARM IVARVFPSSLY M TTRSWD
Sbjct: 61  SVLGSKEKSLESMVYSYKHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMHTTRSWD 120

Query: 121 FLGLSSSPSASSNLLHRAKMGDNVIIGVIDTGMGPIPSRWKGICQSGEDFNSSHCNKKVI 180
           FLGLSSSPSASSNLLHRAKMGDNVIIGVID+GMGPIPSRWKGICQ GEDFNSSHCNKKVI
Sbjct: 121 FLGLSSSPSASSNLLHRAKMGDNVIIGVIDSGMGPIPSRWKGICQLGEDFNSSHCNKKVI 180

Query: 181 GARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYFGNGRGTLRGG 240
           GARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYFGN RGTLRGG
Sbjct: 181 GARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYFGNRRGTLRGG 240

Query: 241 APLARLAIYKVLWSDSLLGSDADILKGIDEAIHDGVDVLSMSIGKSIPLFPVVYDVNPVA 300
           APLARLAIYKV+WSD  LGS ADILKGIDEAIHDGVDVLSMSIG SIPL P V + N VA
Sbjct: 241 APLARLAIYKVIWSDRRLGSAADILKGIDEAIHDGVDVLSMSIGGSIPLPPDVSEWNTVA 300

Query: 301 VGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAATTIDRAFLASITTLGDNATYL 360
           +GSFHA A+G+SVVCAGGNEGSIQQTV NVAPWLFTVAA+TIDRAFL SITTLGDNATYL
Sbjct: 301 IGSFHATARGVSVVCAGGNEGSIQQTVVNVAPWLFTVAASTIDRAFLTSITTLGDNATYL 360

Query: 361 GQTFLKKDIVGKLVVMDRRCAGILGSDIPISGNVVLLCFIDLAMKAAASNAVMPGKQAKV 420
           GQTFLKKD+VGKLVVMDRRCAG LGSDIPI GNVVLLCFIDLA KAAASN VMP KQAKV
Sbjct: 361 GQTFLKKDVVGKLVVMDRRCAGHLGSDIPIGGNVVLLCFIDLAKKAAASNPVMPVKQAKV 420

Query: 421 VGVIYAGQHNDILGSGDIPSIYVDTHPDIAAPGANIIAAVPPNHKGGDKGFAAMSGTSMA 480
           VGVIYAGQH DILG  D+P I+VDTHPDIAAPGANIIAAVPPNHKGGDKGFAAMSGTSMA
Sbjct: 421 VGVIYAGQHTDILGPCDVPCIHVDTHPDIAAPGANIIAAVPPNHKGGDKGFAAMSGTSMA 480

Query: 481 TPHISGIVALIKSLRPTWSPAAIKSALITTARVEDLSEMPIFAEGSPRKVADPFDYGGGV 540
           TPHISGIVALIKSLRPTWSPAAIKSALITTARV+D S +PIFAEGSP KVADPFDYGGGV
Sbjct: 481 TPHISGIVALIKSLRPTWSPAAIKSALITTARVDDPSGLPIFAEGSPPKVADPFDYGGGV 540

Query: 541 VDANAAADPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPSKRASILDLNLPTI 600
           VDANAA DPGLIYDLGATDYIYYYLCSMGYT+A+ISHLSQQKTVCPSKRASILDLNLPTI
Sbjct: 541 VDANAAVDPGLIYDLGATDYIYYYLCSMGYTKAEISHLSQQKTVCPSKRASILDLNLPTI 600

Query: 601 TIPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKKISFKVKFS 660
           T+P LTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKKISFKVKFS
Sbjct: 601 TVPTLTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKKISFKVKFS 660

Query: 661 STLQRNYGYSFGRLTWTDGVHLVKSPLSVGFDFF 692
           STLQRNYGYSFG LTWTDGVHLVKSPLSV FDFF
Sbjct: 661 STLQRNYGYSFGSLTWTDGVHLVKSPLSVRFDFF 694

BLAST of Csor.00g172040 vs. ExPASy TrEMBL
Match: A0A6J1EYT7 (subtilisin-like protease SBT3.9 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111437654 PE=3 SV=1)

HSP 1 Score: 1300 bits (3364), Expect = 0.0
Identity = 681/762 (89.37%), Postives = 686/762 (90.03%), Query Frame = 0

Query: 1   MENNRASSFMHAVVTIYAVFSAMAEADNQNSKVHIVYLGERPYEDVKLTTDSHHELLESV 60
           MENNRASSFMHAVVTIYAVFSAMAEADNQNSKV+I YLGERPYEDVKLTTDSHHELLESV
Sbjct: 1   MENNRASSFMHAVVTIYAVFSAMAEADNQNSKVYIAYLGERPYEDVKLTTDSHHELLESV 60

Query: 61  MGSKEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARMSIVARVFPSSLYNMRTTRSWDFL 120
           MGSKEKSLESM+YSYRHGFSGFAAKLTNSQAQKLARM IVARVFPSSLY MRTTRSWDFL
Sbjct: 61  MGSKEKSLESMMYSYRHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMRTTRSWDFL 120

Query: 121 GLSSSPSASSNLLHRAKMGDNVIIGVIDTG------------MGPIPSRWKGICQSGEDF 180
           GLSSSPSASSNLLHRAKMGDNVIIGVIDTG            MGPIPSRWKGICQSGEDF
Sbjct: 121 GLSSSPSASSNLLHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGICQSGEDF 180

Query: 181 NSSHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYF 240
           NSSHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYF
Sbjct: 181 NSSHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYF 240

Query: 241 GNGRGTLRGGAPLARLAIYKVLWSDSLLGSDADILKGIDEAIHDGVDVLSMSIGKSIPLF 300
           GNGRGTLRGGAPLARLAIYKVLWSDSLLGSDADILKGIDEAIHDGVDVLSMSIGKSIPLF
Sbjct: 241 GNGRGTLRGGAPLARLAIYKVLWSDSLLGSDADILKGIDEAIHDGVDVLSMSIGKSIPLF 300

Query: 301 PVVYDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAATTIDRAFLASI 360
           PVVYDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAA+TIDRAFLASI
Sbjct: 301 PVVYDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAASTIDRAFLASI 360

Query: 361 TTLGDNATYLGQTFLKKDIVGKLVVMDRRCAGILGSDIPISGNVVLLCFIDLAMKAAASN 420
           TTLGDNATYLGQTFLKKD+VGKLVVMDRRCAGILGSDIPISGNVVLLCFIDLAMKA ASN
Sbjct: 361 TTLGDNATYLGQTFLKKDVVGKLVVMDRRCAGILGSDIPISGNVVLLCFIDLAMKAGASN 420

Query: 421 AVMPGKQAKVVGVIYAGQHNDILGSGDIPSIYVDTH------------------------ 480
           AVMPGKQAKVVGVIYAGQHNDILGSGDIPSIYVDTH                        
Sbjct: 421 AVMPGKQAKVVGVIYAGQHNDILGSGDIPSIYVDTHVGTKLFNYLITDDHALIRLTATQT 480

Query: 481 ---------------------------PDIAAPGANIIAAVPPNHKGGDKGFAAMSGTSM 540
                                      PDIAAPG+NIIAAVPPNHKGGDKGFAAMSGTSM
Sbjct: 481 IIGKPISSRIAFFSSRGPNSVSPGILKPDIAAPGSNIIAAVPPNHKGGDKGFAAMSGTSM 540

Query: 541 ATPHIS-------GIVALIKSLRPTWSPAAIKSALITTARVEDLSEMPIFAEGSPRKVAD 600
           ATPHIS       GIVALIKSLRPTWSPAAIKSALITTARVEDLSEMPIFAEGSPRKVAD
Sbjct: 541 ATPHISMATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDLSEMPIFAEGSPRKVAD 600

Query: 601 PFDYGGGVVDANAAADPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPSKRASI 660
           PFDYGGGVVDANAAADPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVC SKRASI
Sbjct: 601 PFDYGGGVVDANAAADPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCSSKRASI 660

Query: 661 LDLNLPTITIPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKK 692
           LDLNLPTITIPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKK
Sbjct: 661 LDLNLPTITIPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKK 720

BLAST of Csor.00g172040 vs. ExPASy TrEMBL
Match: A0A6J1EUE2 (subtilisin-like protease SBT3.9 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111437654 PE=3 SV=1)

HSP 1 Score: 1288 bits (3333), Expect = 0.0
Identity = 677/762 (88.85%), Postives = 683/762 (89.63%), Query Frame = 0

Query: 1   MENNRASSFMHAVVTIYAVFSAMAEADNQNSKVHIVYLGERPYEDVKLTTDSHHELLESV 60
           MENNRASSFMHAVVTIYAVFSAMAEADNQNSKV+I YLGERPYEDVKLTTDSHHELLESV
Sbjct: 1   MENNRASSFMHAVVTIYAVFSAMAEADNQNSKVYIAYLGERPYEDVKLTTDSHHELLESV 60

Query: 61  MGSKEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARMSIVARVFPSSLYNMRTTRSWDFL 120
           MGSKEKSLESM+YSYRHGFSGFAAKLTNSQAQKLA    +ARVFPSSLY MRTTRSWDFL
Sbjct: 61  MGSKEKSLESMMYSYRHGFSGFAAKLTNSQAQKLA----LARVFPSSLYKMRTTRSWDFL 120

Query: 121 GLSSSPSASSNLLHRAKMGDNVIIGVIDTG------------MGPIPSRWKGICQSGEDF 180
           GLSSSPSASSNLLHRAKMGDNVIIGVIDTG            MGPIPSRWKGICQSGEDF
Sbjct: 121 GLSSSPSASSNLLHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGICQSGEDF 180

Query: 181 NSSHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYF 240
           NSSHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYF
Sbjct: 181 NSSHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYF 240

Query: 241 GNGRGTLRGGAPLARLAIYKVLWSDSLLGSDADILKGIDEAIHDGVDVLSMSIGKSIPLF 300
           GNGRGTLRGGAPLARLAIYKVLWSDSLLGSDADILKGIDEAIHDGVDVLSMSIGKSIPLF
Sbjct: 241 GNGRGTLRGGAPLARLAIYKVLWSDSLLGSDADILKGIDEAIHDGVDVLSMSIGKSIPLF 300

Query: 301 PVVYDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAATTIDRAFLASI 360
           PVVYDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAA+TIDRAFLASI
Sbjct: 301 PVVYDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAASTIDRAFLASI 360

Query: 361 TTLGDNATYLGQTFLKKDIVGKLVVMDRRCAGILGSDIPISGNVVLLCFIDLAMKAAASN 420
           TTLGDNATYLGQTFLKKD+VGKLVVMDRRCAGILGSDIPISGNVVLLCFIDLAMKA ASN
Sbjct: 361 TTLGDNATYLGQTFLKKDVVGKLVVMDRRCAGILGSDIPISGNVVLLCFIDLAMKAGASN 420

Query: 421 AVMPGKQAKVVGVIYAGQHNDILGSGDIPSIYVDTH------------------------ 480
           AVMPGKQAKVVGVIYAGQHNDILGSGDIPSIYVDTH                        
Sbjct: 421 AVMPGKQAKVVGVIYAGQHNDILGSGDIPSIYVDTHVGTKLFNYLITDDHALIRLTATQT 480

Query: 481 ---------------------------PDIAAPGANIIAAVPPNHKGGDKGFAAMSGTSM 540
                                      PDIAAPG+NIIAAVPPNHKGGDKGFAAMSGTSM
Sbjct: 481 IIGKPISSRIAFFSSRGPNSVSPGILKPDIAAPGSNIIAAVPPNHKGGDKGFAAMSGTSM 540

Query: 541 ATPHIS-------GIVALIKSLRPTWSPAAIKSALITTARVEDLSEMPIFAEGSPRKVAD 600
           ATPHIS       GIVALIKSLRPTWSPAAIKSALITTARVEDLSEMPIFAEGSPRKVAD
Sbjct: 541 ATPHISMATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDLSEMPIFAEGSPRKVAD 600

Query: 601 PFDYGGGVVDANAAADPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPSKRASI 660
           PFDYGGGVVDANAAADPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVC SKRASI
Sbjct: 601 PFDYGGGVVDANAAADPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCSSKRASI 660

Query: 661 LDLNLPTITIPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKK 692
           LDLNLPTITIPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKK
Sbjct: 661 LDLNLPTITIPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKK 720

BLAST of Csor.00g172040 vs. ExPASy TrEMBL
Match: A0A6J1EUE7 (subtilisin-like protease SBT3.9 isoform X7 OS=Cucurbita moschata OX=3662 GN=LOC111437654 PE=3 SV=1)

HSP 1 Score: 1262 bits (3266), Expect = 0.0
Identity = 661/744 (88.84%), Postives = 668/744 (89.78%), Query Frame = 0

Query: 19  VFSAMAEADNQNSKVHIVYLGERPYEDVKLTTDSHHELLESVMGSKEKSLESMVYSYRHG 78
           +FSAMAEAD+QNSKV+I YLGERPYEDVKLTTDSHHELLESVMGSKEKSLESM+YSYRHG
Sbjct: 1   MFSAMAEADDQNSKVYIAYLGERPYEDVKLTTDSHHELLESVMGSKEKSLESMMYSYRHG 60

Query: 79  FSGFAAKLTNSQAQKLARMSIVARVFPSSLYNMRTTRSWDFLGLSSSPSASSNLLHRAKM 138
           FSGFAAKLTNSQAQKLARM IVARVFPSSLY MRTTRSWDFLGLSSSPSASSNLLHRAKM
Sbjct: 61  FSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMRTTRSWDFLGLSSSPSASSNLLHRAKM 120

Query: 139 GDNVIIGVIDTG------------MGPIPSRWKGICQSGEDFNSSHCNKKVIGARWFARA 198
           GDNVIIGVIDTG            MGPIPSRWKGICQSGEDFNSSHCNKKVIGARWFARA
Sbjct: 121 GDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGICQSGEDFNSSHCNKKVIGARWFARA 180

Query: 199 LIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYFGNGRGTLRGGAPLARLAI 258
           LIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYFGNGRGTLRGGAPLARLAI
Sbjct: 181 LIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYFGNGRGTLRGGAPLARLAI 240

Query: 259 YKVLWSDSLLGSDADILKGIDEAIHDGVDVLSMSIGKSIPLFPVVYDVNPVAVGSFHAIA 318
           YKVLWSDSLLGSDADILKGIDEAIHDGVDVLSMSIGKSIPLFPVVYDVNPVAVGSFHAIA
Sbjct: 241 YKVLWSDSLLGSDADILKGIDEAIHDGVDVLSMSIGKSIPLFPVVYDVNPVAVGSFHAIA 300

Query: 319 KGISVVCAGGNEGSIQQTVENVAPWLFTVAATTIDRAFLASITTLGDNATYLGQTFLKKD 378
           KGISVVCAGGNEGSIQQTVENVAPWLFTVAA+TIDRAFLASITTLGDNATYLGQTFLKKD
Sbjct: 301 KGISVVCAGGNEGSIQQTVENVAPWLFTVAASTIDRAFLASITTLGDNATYLGQTFLKKD 360

Query: 379 IVGKLVVMDRRCAGILGSDIPISGNVVLLCFIDLAMKAAASNAVMPGKQAKVVGVIYAGQ 438
           +VGKLVVMDRRCAGILGSDIPISGNVVLLCFIDLAMKA ASNAVMPGKQAKVVGVIYAGQ
Sbjct: 361 VVGKLVVMDRRCAGILGSDIPISGNVVLLCFIDLAMKAGASNAVMPGKQAKVVGVIYAGQ 420

Query: 439 HNDILGSGDIPSIYVDTH------------------------------------------ 498
           HNDILGSGDIPSIYVDTH                                          
Sbjct: 421 HNDILGSGDIPSIYVDTHVGTKLFNYLITDDHALIRLTATQTIIGKPISSRIAFFSSRGP 480

Query: 499 ---------PDIAAPGANIIAAVPPNHKGGDKGFAAMSGTSMATPHIS-------GIVAL 558
                    PDIAAPG+NIIAAVPPNHKGGDKGFAAMSGTSMATPHIS       GIVAL
Sbjct: 481 NSVSPGILKPDIAAPGSNIIAAVPPNHKGGDKGFAAMSGTSMATPHISMATPHISGIVAL 540

Query: 559 IKSLRPTWSPAAIKSALITTARVEDLSEMPIFAEGSPRKVADPFDYGGGVVDANAAADPG 618
           IKSLRPTWSPAAIKSALITTARVEDLSEMPIFAEGSPRKVADPFDYGGGVVDANAAADPG
Sbjct: 541 IKSLRPTWSPAAIKSALITTARVEDLSEMPIFAEGSPRKVADPFDYGGGVVDANAAADPG 600

Query: 619 LIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPSKRASILDLNLPTITIPALTNSTT 678
           LIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVC SKRASILDLNLPTITIPALTNSTT
Sbjct: 601 LIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCSSKRASILDLNLPTITIPALTNSTT 660

Query: 679 VTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKKISFKVKFSSTLQRNYGYS 692
           VTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKKISFKVKFSSTLQRNYGYS
Sbjct: 661 VTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKKISFKVKFSSTLQRNYGYS 720

BLAST of Csor.00g172040 vs. ExPASy TrEMBL
Match: A0A6J1JBL4 (subtilisin-like protease SBT3.9 OS=Cucurbita maxima OX=3661 GN=LOC111482985 PE=3 SV=1)

HSP 1 Score: 1223 bits (3165), Expect = 0.0
Identity = 644/755 (85.30%), Postives = 653/755 (86.49%), Query Frame = 0

Query: 1   MENNRASSFMHAVVTIYAVFSAMAEADNQNSKVHIVYLGERPYEDVKLTTDSHHELLESV 60
           MENNRA SF+ AVVTIYAVFS MAEAD+QNSKVHIVYLGERPY+DVKLTTDSHHELLESV
Sbjct: 1   MENNRAPSFILAVVTIYAVFSDMAEADDQNSKVHIVYLGERPYDDVKLTTDSHHELLESV 60

Query: 61  MGSKEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARMSIVARVFPSSLYNMRTTRSWDFL 120
           +GSKEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARM IVARVFPSSLY MRTTRSWDFL
Sbjct: 61  LGSKEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMRTTRSWDFL 120

Query: 121 GLSSSPSASSNLLHRAKMGDNVIIGVIDTG------------MGPIPSRWKGICQSGEDF 180
           GLSSSPS SSNL HRAKMGDNVIIGVIDTG            MGPIPSRWKGICQSGEDF
Sbjct: 121 GLSSSPSESSNLFHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGICQSGEDF 180

Query: 181 NSSHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYF 240
           NSSHCNKKVIGARWFA ALIADHGEEAVFKDYLSARDN+GHGTHTASTAGGAFVRNVSYF
Sbjct: 181 NSSHCNKKVIGARWFASALIADHGEEAVFKDYLSARDNEGHGTHTASTAGGAFVRNVSYF 240

Query: 241 GNGRGTLRGGAPLARLAIYKVLWSDSLLGSDADILKGIDEAIHDGVDVLSMSIGKSIPLF 300
           GNGRGTLRGGAPLARLAIYKVLWSDS LGS ADILKGIDEAIHDGVDVLSMSIGKSIPLF
Sbjct: 241 GNGRGTLRGGAPLARLAIYKVLWSDSRLGSGADILKGIDEAIHDGVDVLSMSIGKSIPLF 300

Query: 301 PVVYDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAATTIDRAFLASI 360
           P V DVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAA+TIDRAFL SI
Sbjct: 301 PDVNDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAASTIDRAFLVSI 360

Query: 361 TTLGDNATYLGQTFLKKDIVGKLVVMDRRCAGILGSDIPISGNVVLLCFIDLAMKAAASN 420
           TTLGDNATYLGQ FL+KDIVG LV MD RCAGILG DIPISGNVVLLCF DLA  A ASN
Sbjct: 361 TTLGDNATYLGQMFLRKDIVGMLVAMDGRCAGILGGDIPISGNVVLLCFTDLAKTAPASN 420

Query: 421 AVMPGKQAKVVGVIYAGQHNDILGSGDIPSIYVDTH------------------------ 480
           AVMPGKQAKVVGVIYAGQ  D L   DIP I+VDTH                        
Sbjct: 421 AVMPGKQAKVVGVIYAGQQTDNLVPCDIPCIHVDTHVGTKLFTYLLNDDDALIRLRATRT 480

Query: 481 ---------------------------PDIAAPGANIIAAVPPNHKGGDKGFAAMSGTSM 540
                                      PDIAAPGANIIAAVPPNHKGGDKGFA MSGTSM
Sbjct: 481 IIGKPISSRIAYFSSRGPNSFSPGILKPDIAAPGANIIAAVPPNHKGGDKGFATMSGTSM 540

Query: 541 ATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDLSEMPIFAEGSPRKVADPFDYGGG 600
           ATPHISGIV LIKSL PTWSPAAIKSALITTARVED S MPIFAEGSP KVADPFDYGGG
Sbjct: 541 ATPHISGIVTLIKSLHPTWSPAAIKSALITTARVEDPSGMPIFAEGSPPKVADPFDYGGG 600

Query: 601 VVDANAAADPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPSKRASILDLNLPT 660
           VVDANAA DPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPSKRASILDLNLPT
Sbjct: 601 VVDANAAVDPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPSKRASILDLNLPT 660

Query: 661 ITIPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKKISFKVKF 692
           IT+PAL NSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKKISFKVKF
Sbjct: 661 ITVPALINSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKKISFKVKF 720

BLAST of Csor.00g172040 vs. ExPASy TrEMBL
Match: A0A6J1ETT0 (subtilisin-like protease SBT3.7 isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111437654 PE=3 SV=1)

HSP 1 Score: 1221 bits (3158), Expect = 0.0
Identity = 638/755 (84.50%), Postives = 657/755 (87.02%), Query Frame = 0

Query: 1   MENNRASSFMHAVVTIYAVFSAMAEADNQNSKVHIVYLGERPYEDVKLTTDSHHELLESV 60
           MENNRASSFMHAVVTIYAVFSAMAEADNQNSKV+I YLGERPYEDVKLTTDSHHELLESV
Sbjct: 1   MENNRASSFMHAVVTIYAVFSAMAEADNQNSKVYIAYLGERPYEDVKLTTDSHHELLESV 60

Query: 61  MGSKEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARMSIVARVFPSSLYNMRTTRSWDFL 120
           MGSKEKSLESMVYSY+HGFSGFAAKLTNSQAQKLARM IVARVFPSSLY M TTRSWDFL
Sbjct: 61  MGSKEKSLESMVYSYKHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMHTTRSWDFL 120

Query: 121 GLSSSPSASSNLLHRAKMGDNVIIGVIDTG------------MGPIPSRWKGICQSGEDF 180
           GLSSSPSASSNLLHRAKMGDNVIIGVID+G            MGPIPSRWKGICQ GEDF
Sbjct: 121 GLSSSPSASSNLLHRAKMGDNVIIGVIDSGFWPESESFNDKGMGPIPSRWKGICQLGEDF 180

Query: 181 NSSHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYF 240
           NS HCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYF
Sbjct: 181 NSFHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYF 240

Query: 241 GNGRGTLRGGAPLARLAIYKVLWSDSLLGSDADILKGIDEAIHDGVDVLSMSIGKSIPLF 300
           GN RGTLRGGAPLARLAIYKV+WSD  LGS ADILKGIDEAIHDGVDVLSMSIG SIPL 
Sbjct: 241 GNRRGTLRGGAPLARLAIYKVIWSDRRLGSAADILKGIDEAIHDGVDVLSMSIGGSIPLP 300

Query: 301 PVVYDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAATTIDRAFLASI 360
           P V + N VA+GSFHAIA+G+SVVCAGGNEGSIQQTV NVAPWLFTVAA+TIDRAFL SI
Sbjct: 301 PDVSEWNTVAIGSFHAIARGVSVVCAGGNEGSIQQTVVNVAPWLFTVAASTIDRAFLTSI 360

Query: 361 TTLGDNATYLGQTFLKKDIVGKLVVMDRRCAGILGSDIPISGNVVLLCFIDLAMKAAASN 420
           TTLGDNATYLGQTFLKKD+VGKLVVMDRRCAG LGSDIP  GNVVLLCFIDLAMKAAASN
Sbjct: 361 TTLGDNATYLGQTFLKKDVVGKLVVMDRRCAGHLGSDIPTGGNVVLLCFIDLAMKAAASN 420

Query: 421 AVMPGKQAKVVGVIYAGQHNDILGSGDIPSIYVDTH------------------------ 480
            VMP KQAKVVGVIYAGQH DILG  D+P I+VDTH                        
Sbjct: 421 PVMPVKQAKVVGVIYAGQHTDILGPCDVPCIHVDTHVGTKLLTYILSDEKALIRLKATRT 480

Query: 481 ---------------------------PDIAAPGANIIAAVPPNHKGGDKGFAAMSGTSM 540
                                      PDIAAPG+NIIAAVPPNHKGGDKGFAAMSGTSM
Sbjct: 481 IVGKPISSRIAYFSSRGPNSVSPGILKPDIAAPGSNIIAAVPPNHKGGDKGFAAMSGTSM 540

Query: 541 ATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDLSEMPIFAEGSPRKVADPFDYGGG 600
           ATPHISGIVALIKSLRPTWSPAAIKSALITTARVED S +PIFAEGSP KVADPFDYGGG
Sbjct: 541 ATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDPSGLPIFAEGSPPKVADPFDYGGG 600

Query: 601 VVDANAAADPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPSKRASILDLNLPT 660
           VVDANAA DPGLIYDLGATDYIYYY+CSMGYT+A+ISHLSQQK VCPSKRAS+LDLNLPT
Sbjct: 601 VVDANAAVDPGLIYDLGATDYIYYYMCSMGYTKAEISHLSQQKIVCPSKRASVLDLNLPT 660

Query: 661 ITIPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKKISFKVKF 692
           IT+PALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRV PRVLVFNSNVKKISFKVKF
Sbjct: 661 ITVPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVNPRVLVFNSNVKKISFKVKF 720

BLAST of Csor.00g172040 vs. TAIR 10
Match: AT4G10520.1 (Subtilase family protein )

HSP 1 Score: 601.3 bits (1549), Expect = 9.9e-172
Identity = 351/738 (47.56%), Postives = 456/738 (61.79%), Query Frame = 0

Query: 31  SKVHIVYLGERPYEDVKLTTDSHHELLESVMGSKEKSLESMVYSYRHGFSGFAAKLTNSQ 90
           SKV++VYLGE+ +++ +  T+SHH++L S++GSKE  L+S+VYSYRHGFSGFAAKLT SQ
Sbjct: 27  SKVYVVYLGEKEHDNPESVTESHHQMLWSLLGSKEAVLDSIVYSYRHGFSGFAAKLTESQ 86

Query: 91  AQKLARMSIVARVFPSSLYNMRTTRSWDFLGLSSSPSASSNLLHRAKMGDNVIIGVIDTG 150
           AQ+++ +  V +V P++LY M TTR+WD+LG+  SP  S +LL +A MG NVI+GVID+G
Sbjct: 87  AQQISELPEVVQVIPNTLYEMTTTRTWDYLGV--SPGNSDSLLQKANMGYNVIVGVIDSG 146

Query: 151 M------------GPIPSRWKGICQSGEDFNSS-HCNKKVIGARWFARALIADHG--EEA 210
           +            GPIPSRWKG C+SGE FN+S HCN+K+IGA++F   L+A+ G     
Sbjct: 147 VWPESEMFNDKGFGPIPSRWKGGCESGELFNASIHCNRKLIGAKYFVDGLVAEFGVVNRT 206

Query: 211 VFKDYLSARDNKGHGTHTASTAGGAFVRNVSYFGNGRGTLRGGAPLARLAIYKVLWSDSL 270
              +YLS RD  GHGTH AST GG+F+ NVSY G GRGT RGGAP   +A+YK  WS   
Sbjct: 207 QNPEYLSPRDFAGHGTHVASTIGGSFLPNVSYVGLGRGTARGGAPGVHIAVYKACWSGYC 266

Query: 271 LGSDADILKGIDEAIHDGVDVLSMSIGKSIPLFPVVYDVNPVAVGSFHAIAKGISVVCAG 330
            G  AD+LK +DEAIHDGVD+LS+S+G S+PLFP   +    +VG+FHA+AKGI VV A 
Sbjct: 267 SG--ADVLKAMDEAIHDGVDILSLSLGPSVPLFP---ETEHTSVGAFHAVAKGIPVVIAA 326

Query: 331 GNEGSIQQTVENVAPWLFTVAATTIDRAFLASITTLGDNATYLGQTFLKKDIVGKLVVMD 390
           GN G   QT+ NVAPW+ TVAATT DR+F  +I TLG+N T LGQ       +G      
Sbjct: 327 GNAGPTAQTISNVAPWVLTVAATTQDRSFPTAI-TLGNNITILGQAIYGGPELG------ 386

Query: 391 RRCAGILGSDIPISGNV--------------VLLCFIDLAMKAAASNAVMPGKQAKVVGV 450
               G+   + P+SG+               V+LCF       AA  AV+    A  +G+
Sbjct: 387 --FVGLTYPESPLSGDCEKLSANPNSTMEGKVVLCFAASTPSNAAIAAVI---NAGGLGL 446

Query: 451 IYA-GQHNDILGSGDIPSIYVD-------------------------------------- 510
           I A    + +  +   P + +D                                      
Sbjct: 447 IMAKNPTHSLTPTRKFPWVSIDFELGTDILFYIRSTRSPIVKIQASKTLFGQSVSTKVAT 506

Query: 511 -------------THPDIAAPGANIIAAVPPNHKGGDKGFAAMSGTSMATPHISGIVALI 570
                          PDIAAPG NI+AA+ PN    D GFA MSGTSMATP +SG+V L+
Sbjct: 507 FSSRGPNSVSPAILKPDIAAPGVNILAAISPNSSINDGGFAMMSGTSMATPVVSGVVVLL 566

Query: 571 KSLRPTWSPAAIKSALITTARVEDLSEMPIFAEGSPRKVADPFDYGGGVVDANAAADPGL 630
           KSL P WSP+AIKSA++TTA   D S  PIFA+GS RK+ADPFDYGGG+++   A  PGL
Sbjct: 567 KSLHPDWSPSAIKSAIVTTAWRTDPSGEPIFADGSSRKLADPFDYGGGLINPEKAVKPGL 626

Query: 631 IYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPSKRASILDLNLPTITIPALTNSTTV 688
           IYD+   DY+  Y+CS+ Y++  IS +  + TVCP+ + S+LDLNLP+ITIP L    T+
Sbjct: 627 IYDMTTDDYV-MYMCSVDYSDISISRVLGKITVCPNPKPSVLDLNLPSITIPNLRGEVTL 686

BLAST of Csor.00g172040 vs. TAIR 10
Match: AT4G10510.1 (Subtilase family protein )

HSP 1 Score: 600.9 bits (1548), Expect = 1.3e-171
Identity = 347/738 (47.02%), Postives = 450/738 (60.98%), Query Frame = 0

Query: 33  VHIVYLGERPYEDVKLTTDSHHELLESVMGSKEKSLESMVYSYRHGFSGFAAKLTNSQAQ 92
           VHIVYLGE+ ++D +  T+SHH +L S++GSKE++  SMV+S+RHGFSGFAAKLT SQA+
Sbjct: 22  VHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEEAHGSMVHSFRHGFSGFAAKLTESQAK 81

Query: 93  KLARMSIVARVFPSSLYNMRTTRSWDFLGLSSSPSASSNLLHRAKMGDNVIIGVIDTG-- 152
           K+A +  V  V P   Y   TTR+WD+LGL  SP+   NLL++  MG+ +IIG+ID+G  
Sbjct: 82  KIADLPEVVHVIPDRFYKPATTRTWDYLGL--SPTNPKNLLNQTNMGEQMIIGIIDSGVW 141

Query: 153 ----------MGPIPSRWKGICQSGEDFNSSHCNKKVIGARWFARALIADHG--EEAVFK 212
                     +GP+PS WKG C+SGEDFNSSHCNKK+IGA++F  A +A H     +   
Sbjct: 142 PESEVFNDNEIGPVPSHWKGGCESGEDFNSSHCNKKLIGAKYFINAFLATHESFNSSESL 201

Query: 213 DYLSARDNKGHGTHTASTAGGAFVRNVSYFGNGRGTLRGGAPLARLAIYKVLW---SDSL 272
           D++S R   GHGTH A+ AGG++V N SY G   GT+RGGAP AR+A+YK  W    D  
Sbjct: 202 DFISPRGYNGHGTHVATIAGGSYVPNTSYKGLAGGTVRGGAPRARIAVYKTCWYLDLDIA 261

Query: 273 LGSDADILKGIDEAIHDGVDVLSMSIGKSIPLFPVVYDVNPVAVGSFHAIAKGISVVCAG 332
             S ADILK +DEAIHDGVDVLS+S+G   PL+P     + +A G+FHA+ KGI+VVCA 
Sbjct: 262 ACSSADILKAMDEAIHDGVDVLSLSLGFE-PLYPETDVRDGIATGAFHAVLKGITVVCAA 321

Query: 333 GNEGSIQQTVENVAPWLFTVAATTIDRAFLASITTLGDNATYLGQTFLKKDIVGKLVVMD 392
           GN G   QTV N APW+ TVAATT+DR+F+  + TLG+N   LGQ       VG   ++ 
Sbjct: 322 GNAGPAAQTVGNTAPWILTVAATTLDRSFVTPM-TLGNNKVILGQAIYTGTEVGFTSLVY 381

Query: 393 RRCAG-------------ILGSDIPISGNVVLLCFIDLAMKAAASNAVMPGKQAKVVGVI 452
               G             ++ S+  ++G VV LCF +     + + A    K+A  +GVI
Sbjct: 382 PENPGNSNESFSGTCERLLINSNRTMAGKVV-LCFTESPYSISVTRAAHYVKRAGGLGVI 441

Query: 453 YAGQHNDILGS--GDIPSIYVD-------------------------------------- 512
            AGQ  ++L     D P + VD                                      
Sbjct: 442 IAGQPGNVLRPCLDDFPCVAVDYELGTYILFYIRSNGSPVVKIQPSRTLIGQPVGTKVAS 501

Query: 513 -------------THPDIAAPGANIIAAVPPNHKGGDKGFAAMSGTSMATPHISGIVALI 572
                          PDIAAPG +I+AA   N    D+GF  +SGTSMATP ISGIVAL+
Sbjct: 502 FSSRGPNPISAAILKPDIAAPGVSILAATTTNTTFNDRGFIFLSGTSMATPTISGIVALL 561

Query: 573 KSLRPTWSPAAIKSALITTARVEDLSEMPIFAEGSPRKVADPFDYGGGVVDANAAADPGL 632
           K+L P WSPAAI+SA++TTA   D     IFAEGSPRK ADPFDYGGG+V+   A  PGL
Sbjct: 562 KALHPDWSPAAIRSAIVTTAWRTDPFGEQIFAEGSPRKPADPFDYGGGLVNPEKATKPGL 621

Query: 633 IYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPSKRASILDLNLPTITIPALTNSTTV 688
           +YDLG  DY+  Y+CS+GY E  IS L  + TVC   + S+LD NLP+ITIP L    T+
Sbjct: 622 VYDLGLEDYV-LYMCSVGYNETSISQLVGKGTVCSYPKPSVLDFNLPSITIPNLKEEVTL 681

BLAST of Csor.00g172040 vs. TAIR 10
Match: AT4G10540.1 (Subtilase family protein )

HSP 1 Score: 600.1 bits (1546), Expect = 2.2e-171
Identity = 357/771 (46.30%), Postives = 459/771 (59.53%), Query Frame = 0

Query: 1   MENNRASSFMHAVVTIYAVFSAMAEADNQNSKVHIVYLGERPYEDVKLTTDSHHELLESV 60
           M++ R   F+  ++   + F A A A+   SKVHIVYLGE+ ++D +  T+SHH +L S+
Sbjct: 1   MKSCRTLIFVAIILNGLSTFVAHAGAE---SKVHIVYLGEKQHDDPEFVTESHHRMLWSL 60

Query: 61  MGSKEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARMSIVARVFPSSLYNMRTTRSWDFL 120
           +GSKE +  SMV+SYRHGFSGFAAKLT SQA+KLA +  V  V P S Y + TTR+WD+L
Sbjct: 61  LGSKEDAHSSMVHSYRHGFSGFAAKLTKSQAKKLADLPEVVHVTPDSFYQLDTTRTWDYL 120

Query: 121 GLSSSPSASSNLLHRAKMGDNVIIGVIDT------------GMGPIPSRWKGICQSGEDF 180
           GL  S +   NLL+   MG+ VIIG++D+            G+GP+PS WKG C SGE+F
Sbjct: 121 GL--SVANPKNLLNDTNMGEEVIIGIVDSGVWPESEVFNDNGIGPVPSHWKGGCVSGENF 180

Query: 181 NSSHCNKKVIGARWFARALIADHG--EEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVS 240
            SS CNKK+IGA++F    +A H         D++S RD  GHGTH A+ AGG++V ++S
Sbjct: 181 TSSQCNKKLIGAKYFINGFLATHESFNSTESLDFISPRDRSGHGTHVATIAGGSYVPSIS 240

Query: 241 YFGNGRGTLRGGAPLARLAIYKVLW----SDSLLGSDADILKGIDEAIHDGVDVLSMSIG 300
           Y G   GT+RGGAP AR+A+YK  W     D    S ADILK +DEA+HDGVDVLS+SIG
Sbjct: 241 YKGLAGGTVRGGAPRARIAMYKACWYLDRFDINTCSSADILKAMDEAMHDGVDVLSLSIG 300

Query: 301 KSIPLFPVVYDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAATTIDR 360
              P FP       +A G+FHA+ KGI+VVC+GGN G   QTV N APW+ TVAATT+DR
Sbjct: 301 YRFPYFPETDVRAVIATGAFHAVLKGITVVCSGGNSGPAAQTVGNTAPWILTVAATTLDR 360

Query: 361 AFLASITTLGDNATYLGQTFLKKDIVGKLVVMDRRCAG-------------ILGSDIPIS 420
           +F   I TLG+N   LGQ       +G   ++     G                S+  ++
Sbjct: 361 SFPTPI-TLGNNKLILGQAMYTGPELGFTSLVYPENPGNSNESFSGDCELLFFNSNHTMA 420

Query: 421 GNVVLLCFIDLAMKAAASNAVMPGKQAKVVGVIYAGQHNDILG--SGDIPSIYVD----- 480
           G VV LCF         S+AV   K+A  +GVI A    D L     D P + VD     
Sbjct: 421 GKVV-LCFTTSTRYITVSSAVSYVKEAGGLGVIVARNPGDNLSPCEDDFPCVAVDYELGT 480

Query: 481 ----------------------------------------------THPDIAAPGANIIA 540
                                                           PDIAAPG +I+A
Sbjct: 481 DILLYIRSTGLPVVKIQPSKTLVGQPVGTKVADFSSRGPNSIEPAILKPDIAAPGVSILA 540

Query: 541 AVPPNHKGGDKGFAAMSGTSMATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDLSE 600
           A   N    D+GF  +SGTSMA P ISG+VAL+K+L   WSPAAI+SA++TTA   D   
Sbjct: 541 ATTTNKTFNDRGFIFLSGTSMAAPTISGVVALLKALHRDWSPAAIRSAIVTTAWRTDPFG 600

Query: 601 MPIFAEGSPRKVADPFDYGGGVVDANAAADPGLIYDLGATDYIYYYLCSMGYTEADISHL 660
             IFAEGSPRK+ADPFDYGGG+V+   AA PGL+YDLG  DY+  Y+CS+GY E  IS L
Sbjct: 601 EQIFAEGSPRKLADPFDYGGGLVNPEKAAKPGLVYDLGLEDYV-LYMCSVGYNETSISQL 660

Query: 661 SQQKTVCPSKRASILDLNLPTITIPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVR 688
             + TVC + + S+LD NLP+ITIP L +  T+TRT+TNVG L +VYK VI+ P G +V 
Sbjct: 661 VGKGTVCSNPKPSVLDFNLPSITIPNLKDEVTLTRTLTNVGQLESVYKVVIEPPIGIQVT 720

BLAST of Csor.00g172040 vs. TAIR 10
Match: AT1G32960.1 (Subtilase family protein )

HSP 1 Score: 595.9 bits (1535), Expect = 4.2e-170
Identity = 358/765 (46.80%), Postives = 462/765 (60.39%), Query Frame = 0

Query: 7   SSFMHAVVTIYAVFSAMAEADNQNSKVHIVYLGERPYEDVKLTTDSHHELLESVMGSKEK 66
           SS +  ++++  V +A        SKVHIVYLGE+ + D +  T+SHH++L S++GSK+ 
Sbjct: 6   SSILLVLLSLITVLNATRARSETESKVHIVYLGEKKHHDPEFVTESHHQMLASLLGSKKD 65

Query: 67  SLESMVYSYRHGFSGFAAKLTNSQAQKLARMSIVARVFPSSLYNMRTTRSWDFLGLSSSP 126
           + +SMVYSYRHGFSGFAAKLT SQA+K+A +  V  V P   + + TTR+W++LGLSS  
Sbjct: 66  ADDSMVYSYRHGFSGFAAKLTKSQAKKIADLPEVVHVIPDGFHELATTRTWEYLGLSS-- 125

Query: 127 SASSNLLHRAKMGDNVIIGVIDT------------GMGPIPSRWKGICQSGEDFNSSHCN 186
           +   NLL+   MGD VIIGVIDT            G+GPIP +WKG C+SGE+F S+ CN
Sbjct: 126 ANPKNLLNDTNMGDQVIIGVIDTGVWPESESFNDNGVGPIPRKWKGGCESGENFRSTDCN 185

Query: 187 KKVIGARWFARALIADHG--EEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYFGNGR 246
           +K+IGA++F    +A++        +DY+SARD  GHGTH AS AGG+FV NVSY G   
Sbjct: 186 RKLIGAKYFINGFLAENKGFNTTESRDYISARDFDGHGTHVASIAGGSFVPNVSYKGLAG 245

Query: 247 GTLRGGAPLARLAIYKVLW-SDSLLG---SDADILKGIDEAIHDGVDVLSMSIGKSIPLF 306
           GTLRGGAP AR+A+YK  W  + L G   SD+DI+K IDEAIHDGVDVLS+S+   IPL 
Sbjct: 246 GTLRGGAPRARIAMYKACWFHEELKGVTCSDSDIMKAIDEAIHDGVDVLSISLVGQIPLN 305

Query: 307 PVVYDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAATTIDRAFLASI 366
                 +  A G FHA+AKGI VVCAGGN+G   QTV N+APW+ TVAATT+DR+F   I
Sbjct: 306 SETDIRDEFATGLFHAVAKGIVVVCAGGNDGPAAQTVVNIAPWILTVAATTLDRSFPTPI 365

Query: 367 TTLGDNATYLGQTFLKKDIVGKLVVM--------DRRCAGILGSDIPISGNV-----VLL 426
            TLG+N   LGQ       +G   ++        +   +G+  S + ++ N      V+L
Sbjct: 366 -TLGNNKVILGQATYTGPELGLTSLVYPENARNNNETFSGVCES-LNLNPNYTMAMKVVL 425

Query: 427 CFIDLAMKAAASNAVMPGKQAKVVGVIYAGQHNDILG--SGDIPSIYVD----------- 486
           CF      AA S A    K A  +G+I +      L   + D P + VD           
Sbjct: 426 CFTASRTNAAISRAASFVKAAGGLGLIISRNPVYTLSPCNDDFPCVAVDYELGTDILSYI 485

Query: 487 ----------------------------------------THPDIAAPGANIIAAVPPNH 546
                                                     PDIAAPG  I+AA  PN 
Sbjct: 486 RSTRSPVVKIQRSRTLSGQPVGTKVVNFSSRGPNSMSPAILKPDIAAPGVRILAATSPND 545

Query: 547 KGGDKGFAAMSGTSMATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDLSEMPIFAE 606
                GFA +SGTSMATP ISG++AL+K+L P WSPAA +SA++TTA   D     IFAE
Sbjct: 546 TLNVGGFAMLSGTSMATPVISGVIALLKALHPEWSPAAFRSAIVTTAWRTDPFGEQIFAE 605

Query: 607 GSPRKVADPFDYGGGVVDANAAADPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTV 666
           GS RKV+DPFDYGGG+V+   AA+PGLIYD+G  DYI  YLCS GY ++ IS L  Q TV
Sbjct: 606 GSSRKVSDPFDYGGGIVNPEKAAEPGLIYDMGPQDYI-LYLCSAGYNDSSISQLVGQITV 665

Query: 667 CPSKRASILDLNLPTITIPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVL 688
           C + + S+LD+NLP+ITIP L +  T+TRTVTNVG + +VYK  ++ P G +V V P  L
Sbjct: 666 CSNPKPSVLDVNLPSITIPNLKDEVTLTRTVTNVGLVDSVYKVSVEPPLGVRVVVTPETL 725

BLAST of Csor.00g172040 vs. TAIR 10
Match: AT1G32940.1 (Subtilase family protein )

HSP 1 Score: 593.6 bits (1529), Expect = 2.1e-169
Identity = 351/759 (46.25%), Postives = 455/759 (59.95%), Query Frame = 0

Query: 13  VVTIYAVFSAMAEADNQNSKVHIVYLGERPYEDVKLTTDSHHELLESVMGSKEKSLESMV 72
           V+++  V + +  +D   SKVHIVYLGE+ ++D +  ++SHH++L S++GSK  + ESMV
Sbjct: 11  VLSLVIVLNVVRASD--ESKVHIVYLGEKQHDDPEFVSESHHQMLSSLLGSKVDAHESMV 70

Query: 73  YSYRHGFSGFAAKLTNSQAQKLARMSIVARVFPSSLYNMRTTRSWDFLGLSSSPSASSNL 132
           YSYRHGFSGFAAKLT SQA+KLA    V  V   S Y + TTR+WD+LGL  S +  +NL
Sbjct: 71  YSYRHGFSGFAAKLTESQAKKLADSPEVVHVMADSFYELATTRTWDYLGL--SVANPNNL 130

Query: 133 LHRAKMGDNVIIGVIDT------------GMGPIPSRWKGICQSGEDFNSSHCNKKVIGA 192
           L+   MGD VIIG IDT            G+GPIPS WKG C+SGE F S++CN+K+IGA
Sbjct: 131 LNDTNMGDQVIIGFIDTGVWPESESFNDNGVGPIPSHWKGGCESGEKFISTNCNRKLIGA 190

Query: 193 RWFARALIADHG--EEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYFGNGRGTLRGG 252
           ++F    +A++        +DY+SARD  GHGTHTAS AGG+FV N+SY G   G LRGG
Sbjct: 191 KYFINGFLAENEGFNTTESRDYISARDFIGHGTHTASIAGGSFVPNISYKGLAGGNLRGG 250

Query: 253 APLARLAIYKVLWSDSLLG----SDADILKGIDEAIHDGVDVLSMSIGKSIPLFPVVYDV 312
           AP AR+AIYK  W    LG    S +DILK +DE++HDGVDVLS+S+G  IPL+P     
Sbjct: 251 APRARIAIYKACWYVDQLGAVACSSSDILKAMDESMHDGVDVLSLSLGAQIPLYPETDLR 310

Query: 313 NPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAATTIDRAFLASITTLGDN 372
           + +A G+FHA+AKGI VVCAGGN G   QTV N APW+ TVAATT+DR+F   I TLG+ 
Sbjct: 311 DRIATGAFHAVAKGIIVVCAGGNSGPAAQTVLNTAPWIITVAATTLDRSFPTPI-TLGNR 370

Query: 373 ATYLGQTFLKKDIVGKLVVMDRRCAGI-------------LGSDIPISGNVVLLCFIDLA 432
              LGQ       +G   ++    AG              L  +  ++G VV LCF    
Sbjct: 371 KVILGQALYTGQELGFTSLVYPENAGFTNETFSGVCERLNLNPNRTMAGKVV-LCFTTNT 430

Query: 433 MKAAASNAVMPGKQAKVVGVIYAGQ--HNDILGSGDIPSIYVD----------------- 492
           +  A S A    K A  +GVI A    +N      D P + +D                 
Sbjct: 431 LFTAVSRAASYVKAAGGLGVIIARNPGYNLTPCRDDFPCVAIDYELGTDVLLYIRSTRSP 490

Query: 493 ----------------------------------THPDIAAPGANIIAAVPPNHKGGDKG 552
                                               PDI APG +I+AA  P+      G
Sbjct: 491 VVKIQPSRTLVGQPVGTKVATFSSRGPNSISPAILKPDIGAPGVSILAATSPDSNSSVGG 550

Query: 553 FAAMSGTSMATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDLSEMPIFAEGSPRKV 612
           F  ++GTSMA P ++G+VAL+K+L P WSPAA +SA++TTA   D     IFAEGS RKV
Sbjct: 551 FDILAGTSMAAPVVAGVVALLKALHPNWSPAAFRSAIVTTAWRTDPFGEQIFAEGSSRKV 610

Query: 613 ADPFDYGGGVVDANAAADPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPSKRA 672
           ADPFDYGGG+V+   AADPGLIYD+G  DYI  YLCS GY ++ I+ L    TVC + + 
Sbjct: 611 ADPFDYGGGIVNPEKAADPGLIYDMGPRDYI-LYLCSAGYNDSSITQLVGNVTVCSTPKT 670

Query: 673 SILDLNLPTITIPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNV 688
           S+LD+NLP+ITIP L +  T+TRTVTNVG + +VYK V++ P G +V V P  LVFNS  
Sbjct: 671 SVLDVNLPSITIPDLKDEVTLTRTVTNVGTVDSVYKVVVEPPLGIQVVVAPETLVFNSKT 730