Csor.00g168780 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g168780
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description4-coumarate--CoA ligase-like 1
LocationCsor_Chr18: 8450089 .. 8452403 (-)
RNA-Seq ExpressionCsor.00g168780
SyntenyCsor.00g168780
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATACAAGCTTTCTCAGCTCCTCCTTTAAGAAGAGGGAGACGCCCGTCGATGTCTGCAGCAAACAACAACTCCGCCATGGCTACTTGCATTCGGGATTCAGTCGAAGACGAGGAACACATTTTCCGTAGCCAACTTCCCGAGGTCCAAGTGCCTGATGATATCACATTGCCAGAGTTTGTACTTCAGAATGCTGAATCATATGCTGATAATGTGGCATTTGTGGAAGCTGTGAGCGGAAAGGCGTACACTTATCGTGAAGTTGTGAGAGACACGAGTAGGTTCGCTAAGGCCTTAAGCTCTCTGAGGTTGAAGAAGGGGCAGGTCGTTATTGTCGTTCTACCCAACGTTGCTGAATATGCCATTGTTGCTTTAGGGATAATGGCTGCTGGAGGTGTGTTTTCTGGTGTGAATCCAGCAGCTCACATATCCGAAATCAAAAAGCAGGTGGAGGTAGCAGACGCCAAACTCGTCGTTACAAACAGCTCAAGCTTTGAAAAGGTAAAATCGAAACTGTCGTTATCGATCATATTATTTCGAATTCAACTCGACTTGCTTACTATCTAATGATTGATAACAGGTGAAGGAATTAAAGCTTCCAGTGATCGTAGTAGGGGAGGAACTGATTGAAGGTTCCATGAACTGGCACCAACTGCTCGAAGCTGCGGACCGCGCGGGCAACAATTTCGTCAAAGAAGATATCAAGCAGACTGATTTATGTGCCCTTCCTTTCTCATCAGGCACTACAGGAGTTTCCAAAGGTGTAATGCTAACTCATCGAAATCTAGTGGCAAACTTGTGTTCTACGCTCTCCGGCGTACCGCAAGAAATGGTGGGCAAGGTCACGACGTTAGGCCTCATCCCGTTCTTCCACATTTACGGGATTACCGGAATATGTTGTGCCACACTTCGAAACAAGGGAAAAGTTGTGGTGATGGGAAGATTTGATCTTAGAACCTTCATCAATGCCCTGATAACACAGGAGGTCACATTTGCTCCAATTGTTCCTCCTATCATCTTGGCCTTGGTTAAGAATCCTATTGTGAAGGAATTCGATCTAAGCGGTCTCAAACTTCAGGCTATCATGACTGCAGCTGCTCCGCTCGCACCCGAACTTCAAACGTCCTTTGAGAAAAAGTTCCCGGGCGTGGATGTTCAAGAGGTAATTAAGTTACTACTCGAGTAGATGGATTGTGAGAACTCTCGACTCTTATTCATTCTGTCAAATTATCAGGCTATAACATGTAGTCCACCGTCCTGTTTTCTGCATATCGAAAACGAACCGAGCTCCACCGTTGCATTGTAGAGCTGATTCACAGGGTCATTCTATCTAGGCTAACTAAATGTCATTGATCGCCAACAGGCATATGGGCTAACCGAGCATAGCTGCATCACTCTCAACTATGGAAGCATAGGCAAAGACAATCTCACGCTGCAAAGAAAAACACGGTTGGCCGTATTCTTCCTAATCTAGAAGTCAAATTCATCGACCCCGACAGCGGTAGGTCGCTGCCGAAGAACACACCTGGGGAAATCTGTGTAAGAAGCCAGTGTGTGATGCAAGGTACATAATTTGGACTGGTGCAGAAAATCCATCTGAGATCTCTAATTATATGCTAGTTGAATCTTTCCTTGTTGTTTCAGGCTACTACAATAATGAAGAGGAGACTTCAAGGACCATCGACGACAAAGGCTGGATGCACACGGGCGACATCGGATACATCGACGACGATGGCGATGTGTTTATTGTGGATCGTATTAAAGAGTTGATTAAGTACAAAGGCTTTCAAGTAAAGACACTGCATAAACTTTGCTTGATAAATCGAAGCTCGAGAACAGAAAAAAAGTAGAATTCAAATGGTTATCAAACGAGACCTATATAAGGTAACGAATGTCTTTGATTTCTCTACAGGTAGCTCCGGCCGAGTTAGAGGCAATCCTCCTTACTCATCCCTCGATTGAAGATGCAGCTGTGGTGCCGTAAGCTTCGCTTCCCTTTACATATTTCTATTTAAATGATTTCTGAAACAAATCGAACCCGAAACGGTTAAAACAACGTTAACCCGCGTCAGTATTTCAGGCTGCCAGACAAAGAAGCCGGTGAGATCCCAGCAGCAAGCATTGTAATGACTCCAAATGCAAAAGAAACCGAAGACGAAATAATCAAATACGTCGCCTCAAACGTTGCACATTACAAGAAAGTTAGGCTCGTTCATTTTGTCGAAACGATCCCGAAATCGCCGTCTGGGAAAGTCATGAGGAGGTTGATCAAAGAGAAGATGATCGAAAAGATTCGAGCCGAGACGGCCTCTTGA

mRNA sequence

ATGATACAAGCTTTCTCAGCTCCTCCTTTAAGAAGAGGGAGACGCCCGTCGATGTCTGCAGCAAACAACAACTCCGCCATGGCTACTTGCATTCGGGATTCAGTCGAAGACGAGGAACACATTTTCCGTAGCCAACTTCCCGAGGTCCAAGTGCCTGATGATATCACATTGCCAGAGTTTGTACTTCAGAATGCTGAATCATATGCTGATAATGTGGCATTTGTGGAAGCTGTGAGCGGAAAGGCGTACACTTATCGTGAAGTTGTGAGAGACACGAGTAGGTTCGCTAAGGCCTTAAGCTCTCTGAGGTTGAAGAAGGGGCAGGTCGTTATTGTCGTTCTACCCAACGTTGCTGAATATGCCATTGTTGCTTTAGGGATAATGGCTGCTGGAGGTGTGTTTTCTGGTGTGAATCCAGCAGCTCACATATCCGAAATCAAAAAGCAGGTGGAGGTAGCAGACGCCAAACTCGTCGTTACAAACAGCTCAAGCTTTGAAAAGGTGAAGGAATTAAAGCTTCCAGTGATCGTAGTAGGGGAGGAACTGATTGAAGGTTCCATGAACTGGCACCAACTGCTCGAAGCTGCGGACCGCGCGGGCAACAATTTCGTCAAAGAAGATATCAAGCAGACTGATTTATGTGCCCTTCCTTTCTCATCAGGCACTACAGGAGTTTCCAAAGGTGTAATGCTAACTCATCGAAATCTAGTGGCAAACTTGTGTTCTACGCTCTCCGGCGTACCGCAAGAAATGGTGGGCAAGGTCACGACGTTAGGCCTCATCCCGTTCTTCCACATTTACGGGATTACCGGAATATGTTGTGCCACACTTCGAAACAAGGGAAAAGTTGTGGTGATGGGAAGATTTGATCTTAGAACCTTCATCAATGCCCTGATAACACAGGAGGTCACATTTGCTCCAATTGTTCCTCCTATCATCTTGGCCTTGGTTAAGAATCCTATTGTGAAGGAATTCGATCTAAGCGGTCTCAAACTTCAGGCTATCATGACTGCAGCTGCTCCGCTCGCACCCGAACTTCAAACGTCCTTTGAGAAAAAGTTCCCGGGCGTGGATGTTCAAGAGGCATATGGGCTAACCGAGCATAGCTGCATCACTCTCAACTATGGAAGCATAGGCAAAGACAATCTCACGCTGCAAAGAAAAACACGCGGTAGGTCGCTGCCGAAGAACACACCTGGGGAAATCTGTGTAAGAAGCCAGTGTGTGATGCAAGGCTACTACAATAATGAAGAGGAGACTTCAAGGACCATCGACGACAAAGGCTGGATGCACACGGGCGACATCGGATACATCGACGACGATGGCGATGTGTTTATTGTGGATCGTATTAAAGAGTTGATTAAGTACAAAGGCTTTCAAGTAGCTCCGGCCGAGTTAGAGGCAATCCTCCTTACTCATCCCTCGATTGAAGATGCAGCTGTGGTGCCGCTGCCAGACAAAGAAGCCGGTGAGATCCCAGCAGCAAGCATTGTAATGACTCCAAATGCAAAAGAAACCGAAGACGAAATAATCAAATACGTCGCCTCAAACGTTGCACATTACAAGAAAGTTAGGCTCGTTCATTTTGTCGAAACGATCCCGAAATCGCCGTCTGGGAAAGTCATGAGGAGGTTGATCAAAGAGAAGATGATCGAAAAGATTCGAGCCGAGACGGCCTCTTGA

Coding sequence (CDS)

ATGATACAAGCTTTCTCAGCTCCTCCTTTAAGAAGAGGGAGACGCCCGTCGATGTCTGCAGCAAACAACAACTCCGCCATGGCTACTTGCATTCGGGATTCAGTCGAAGACGAGGAACACATTTTCCGTAGCCAACTTCCCGAGGTCCAAGTGCCTGATGATATCACATTGCCAGAGTTTGTACTTCAGAATGCTGAATCATATGCTGATAATGTGGCATTTGTGGAAGCTGTGAGCGGAAAGGCGTACACTTATCGTGAAGTTGTGAGAGACACGAGTAGGTTCGCTAAGGCCTTAAGCTCTCTGAGGTTGAAGAAGGGGCAGGTCGTTATTGTCGTTCTACCCAACGTTGCTGAATATGCCATTGTTGCTTTAGGGATAATGGCTGCTGGAGGTGTGTTTTCTGGTGTGAATCCAGCAGCTCACATATCCGAAATCAAAAAGCAGGTGGAGGTAGCAGACGCCAAACTCGTCGTTACAAACAGCTCAAGCTTTGAAAAGGTGAAGGAATTAAAGCTTCCAGTGATCGTAGTAGGGGAGGAACTGATTGAAGGTTCCATGAACTGGCACCAACTGCTCGAAGCTGCGGACCGCGCGGGCAACAATTTCGTCAAAGAAGATATCAAGCAGACTGATTTATGTGCCCTTCCTTTCTCATCAGGCACTACAGGAGTTTCCAAAGGTGTAATGCTAACTCATCGAAATCTAGTGGCAAACTTGTGTTCTACGCTCTCCGGCGTACCGCAAGAAATGGTGGGCAAGGTCACGACGTTAGGCCTCATCCCGTTCTTCCACATTTACGGGATTACCGGAATATGTTGTGCCACACTTCGAAACAAGGGAAAAGTTGTGGTGATGGGAAGATTTGATCTTAGAACCTTCATCAATGCCCTGATAACACAGGAGGTCACATTTGCTCCAATTGTTCCTCCTATCATCTTGGCCTTGGTTAAGAATCCTATTGTGAAGGAATTCGATCTAAGCGGTCTCAAACTTCAGGCTATCATGACTGCAGCTGCTCCGCTCGCACCCGAACTTCAAACGTCCTTTGAGAAAAAGTTCCCGGGCGTGGATGTTCAAGAGGCATATGGGCTAACCGAGCATAGCTGCATCACTCTCAACTATGGAAGCATAGGCAAAGACAATCTCACGCTGCAAAGAAAAACACGCGGTAGGTCGCTGCCGAAGAACACACCTGGGGAAATCTGTGTAAGAAGCCAGTGTGTGATGCAAGGCTACTACAATAATGAAGAGGAGACTTCAAGGACCATCGACGACAAAGGCTGGATGCACACGGGCGACATCGGATACATCGACGACGATGGCGATGTGTTTATTGTGGATCGTATTAAAGAGTTGATTAAGTACAAAGGCTTTCAAGTAGCTCCGGCCGAGTTAGAGGCAATCCTCCTTACTCATCCCTCGATTGAAGATGCAGCTGTGGTGCCGCTGCCAGACAAAGAAGCCGGTGAGATCCCAGCAGCAAGCATTGTAATGACTCCAAATGCAAAAGAAACCGAAGACGAAATAATCAAATACGTCGCCTCAAACGTTGCACATTACAAGAAAGTTAGGCTCGTTCATTTTGTCGAAACGATCCCGAAATCGCCGTCTGGGAAAGTCATGAGGAGGTTGATCAAAGAGAAGATGATCGAAAAGATTCGAGCCGAGACGGCCTCTTGA

Protein sequence

MIQAFSAPPLRRGRRPSMSAANNNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHQLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLTLQRKTRGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAETAS
Homology
BLAST of Csor.00g168780 vs. ExPASy Swiss-Prot
Match: Q9LQ12 (4-coumarate--CoA ligase-like 1 OS=Arabidopsis thaliana OX=3702 GN=4CLL1 PE=1 SV=1)

HSP 1 Score: 742.3 bits (1915), Expect = 4.1e-213
Identity = 372/532 (69.92%), Postives = 441/532 (82.89%), Query Frame = 0

Query: 36  EDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRF 95
           ED E+IFRS  P V +PD +TLPEFVLQ  E Y +NVAFVEAV+GKA TY +VVRDT R 
Sbjct: 7   EDNEYIFRSLYPSVPIPDKLTLPEFVLQGVEEYTENVAFVEAVTGKAVTYGDVVRDTKRL 66

Query: 96  AKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADA 155
           AKAL+SL L+KGQV++VVLPNVAEY I+ALGIM+AGGVFSG NP A +SEIKKQVE + A
Sbjct: 67  AKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGA 126

Query: 156 KLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHQLLEAADRAGNNFVKEDIKQTDLCA 215
           + ++T+++++EKVK L LPVIV+GEE IEG++NW  LLEA D+ G+    E+I QTDLCA
Sbjct: 127 RGIITDATNYEKVKSLGLPVIVLGEEKIEGAVNWKDLLEAGDKCGDT-DNEEILQTDLCA 186

Query: 216 LPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCA 275
           LPFSSGTTG+ KGVMLTHRNL+ANLCSTL GV  EM+G++ TLGLIPFFHIYGI GICCA
Sbjct: 187 LPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVRSEMIGQIVTLGLIPFFHIYGIVGICCA 246

Query: 276 TLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAI 335
           T++NKGKVV M R+DLR F+NALI  EV+FAPIVPPIIL LVKNPIV EFDLS LKLQ++
Sbjct: 247 TMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLQSV 306

Query: 336 MTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLTLQRKT------ 395
           MTAAAPLAPEL T+FE KFP V VQEAYGLTEHSCITL +G   K     +R +      
Sbjct: 307 MTAAAPLAPELLTAFEAKFPNVQVQEAYGLTEHSCITLTHGDPEKGQGIAKRNSVGFILP 366

Query: 396 ----------RGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYID 455
                      GRSLPKNT GE+CVRSQCVMQGY+ N+EET +TID++GW+HTGDIGYID
Sbjct: 367 NLEVKFIDPDTGRSLPKNTSGELCVRSQCVMQGYFMNKEETDKTIDEQGWLHTGDIGYID 426

Query: 456 DDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVM 515
           DDGD+FIVDRIKELIKYKGFQVAPAELEAILLTHPS+ED AVVPLPD+EAGEIPAA +V+
Sbjct: 427 DDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDEEAGEIPAACVVI 486

Query: 516 TPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMI 552
            P A E E++I+ +VA+NVAHYKKVR VHFV++IPKS SGK+MRRL+++K++
Sbjct: 487 NPKATEKEEDILNFVAANVAHYKKVRAVHFVDSIPKSLSGKIMRRLLRDKIL 537

BLAST of Csor.00g168780 vs. ExPASy Swiss-Prot
Match: Q7XXL2 (4-coumarate--CoA ligase-like 9 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL9 PE=2 SV=2)

HSP 1 Score: 656.8 bits (1693), Expect = 2.3e-187
Identity = 334/539 (61.97%), Postives = 425/539 (78.85%), Query Frame = 0

Query: 36  EDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVS-GKAYTYREVVRDTSR 95
           E++EH+FRS+ P V VPD +T+PEFVL  AE+YAD VA VEA + G++YTY EV RDT+R
Sbjct: 14  EEQEHVFRSRFPPVAVPDGVTVPEFVLDGAEAYADRVALVEAAAGGRSYTYGEVARDTAR 73

Query: 96  FAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAD 155
           FA+AL S+ ++KG VV+V LPN+A Y +V+LGIM+AG VFSGVNP A  +EIKKQVE ++
Sbjct: 74  FARALRSVGVRKGHVVVVALPNLAVYPVVSLGIMSAGAVFSGVNPRALAAEIKKQVEDSE 133

Query: 156 AKLVVTNSSSFEKVKELKLPVIVVGE-ELIEGSMNWHQLLEAADRAGNNFVKED-IKQTD 215
           AKLVV N  +F+KVK+  +PVI VG+ E + G+++W  LL AADR G   V  D  +Q+D
Sbjct: 134 AKLVVANEVAFDKVKDAGVPVIGVGDRERMPGAISWDGLLAAADRTGAGVVPVDAAQQSD 193

Query: 216 LCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGITGI 275
           LCALP+SSGTTGVSKGVML+HRNLV+NLCS++  V  E  G+V TLGL+PFFHIYGITGI
Sbjct: 194 LCALPYSSGTTGVSKGVMLSHRNLVSNLCSSMFAVAPETAGQVVTLGLMPFFHIYGITGI 253

Query: 276 CCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKL 335
           CCATLR+KG VVVM RFDLRTF+ AL+   V FAP+VPP++LA+VK+P+  EFDLS L L
Sbjct: 254 CCATLRHKGTVVVMDRFDLRTFLRALVDHRVMFAPLVPPVMLAMVKSPVADEFDLSDLAL 313

Query: 336 QAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITL------NYGSIGK------ 395
           +++MTAAAPLAP+L  +F++KFPGV V+EAYGLTEHSCITL       +G + K      
Sbjct: 314 KSVMTAAAPLAPDLLAAFQRKFPGVQVEEAYGLTEHSCITLTHAAGDGHGHVAKKSSVGF 373

Query: 396 --DNLTLQ--RKTRGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIG 455
              NL ++      GRSLP NTPGE+CVRSQ VMQGYY  +EET RT+D KGW+HTGD+G
Sbjct: 374 ILPNLEVKFVDPDTGRSLPANTPGELCVRSQSVMQGYYKRKEETERTVDGKGWLHTGDVG 433

Query: 456 YIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAAS 515
           YID DGDVFIVDRIKELIKYKGFQVAPAELEA+LL+HPS+EDAAV  +PD+EAGE+P A 
Sbjct: 434 YIDGDGDVFIVDRIKELIKYKGFQVAPAELEAVLLSHPSVEDAAVFGVPDEEAGEVPVAC 493

Query: 516 IVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKIR 556
           +V    A+E E+EI+ YVA  VA YK+VR++H V+ IPKS SGK++RR ++++ I++++
Sbjct: 494 VVRRHGAEEGEEEIVAYVAERVASYKRVRVLHIVDAIPKSVSGKILRRQLRDEFIKRMK 552

BLAST of Csor.00g168780 vs. ExPASy Swiss-Prot
Match: P14912 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 4.1e-104
Identity = 202/534 (37.83%), Postives = 322/534 (60.30%), Query Frame = 0

Query: 38  EEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAK 97
           E+ IFRS+LP++ +P  + L  +  +N     D    +   +G+ +TY +V   + + A 
Sbjct: 9   EDLIFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVAS 68

Query: 98  ALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKL 157
            L+ L +++G  ++++LPN  EY    LG    G + +  NP    +E+ KQ++ + AKL
Sbjct: 69  GLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQAKL 128

Query: 158 VVTNSSSFEKVKEL---KLPVIVVGEELIEGSMNWHQLLEAADRAGNNFVKEDIKQTDLC 217
           ++T +   +KVK+    K   I+  ++  +  +++ +L+EA +      V   I   D+ 
Sbjct: 129 IITQACYVDKVKDYAAEKNIQIICIDDAPQDCLHFSKLMEADESEMPEVV---INSDDVV 188

Query: 218 ALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQE--MVGKVTTLGLIPFFHIYGITGI 277
           ALP+SSGTTG+ KGVMLTH+ LV ++   + G      M  +   + ++P FHIY +  +
Sbjct: 189 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAV 248

Query: 278 CCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKL 337
            C  LR    +++M +FD+  F+  +   +VT  P VPPI+LA+ K+P+V ++DLS ++ 
Sbjct: 249 LCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVR- 308

Query: 338 QAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLTLQRKTRG- 397
             +M+ AAPL  EL+ +   KFP   + + YG+TE   +     +  K+   ++    G 
Sbjct: 309 -TVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGT 368

Query: 398 ---------------RSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIG 457
                           SLP+N  GEIC+R   +M+GY N+ E T  TID++GW+HTGDIG
Sbjct: 369 VVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIG 428

Query: 458 YIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAAS 517
           +IDDD ++FIVDR+KE+IKYKGFQVAPAELEA+LLTHP+I DAAVVP+ D++AGE+P A 
Sbjct: 429 FIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAF 488

Query: 518 IVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 551
           +V T     TE+EI ++V+  V  YK++  V FV+ IPKSPSGK++R+ ++ ++
Sbjct: 489 VVRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRARI 537

BLAST of Csor.00g168780 vs. ExPASy Swiss-Prot
Match: P14913 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 7.0e-104
Identity = 203/534 (38.01%), Postives = 322/534 (60.30%), Query Frame = 0

Query: 38  EEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAK 97
           E+ IFRS+LP++ +P  + L  +  +N     D    +   +G+ +TY +V   + + A 
Sbjct: 9   EDLIFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVAS 68

Query: 98  ALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKL 157
            L+ L +++G  ++++LPN  EY    LG    G + +  NP    +E+ KQ++ + AKL
Sbjct: 69  GLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASLAKL 128

Query: 158 VVTNSSSFEKVKEL---KLPVIVVGEELIEGSMNWHQLLEAADRAGNNFVKEDIKQTDLC 217
           ++T +   +KVK+    K   I+  ++  +  +++ +L+EA +      V   I   D+ 
Sbjct: 129 IITQACYVDKVKDYAAEKNIQIICIDDAPQDCLHFSKLMEADESEMPEVV---IDSDDVV 188

Query: 218 ALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQE--MVGKVTTLGLIPFFHIYGITGI 277
           ALP+SSGTTG+ KGVMLTH+ LV ++   + G      M  +   + ++P FHIY +  +
Sbjct: 189 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAV 248

Query: 278 CCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKL 337
            C  LR    +++M +FD+  F+  +   +VT  P VPPI+LA+ K+P+V ++DLS ++ 
Sbjct: 249 LCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVR- 308

Query: 338 QAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLTLQRKTRG- 397
             +M+ AAPL  EL+ +   KFP   + + YG+TE   +     +  K+   ++    G 
Sbjct: 309 -TVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGT 368

Query: 398 ---------------RSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIG 457
                           SLP+N  GEIC+R   +M+GY N+ E T  TID++GW+HTGDIG
Sbjct: 369 VVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIG 428

Query: 458 YIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAAS 517
           +IDDD ++FIVDR+KE+IKYKGFQVAPAELEA+LLTHP+I DAAVVP+ D++AGE+P A 
Sbjct: 429 FIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAF 488

Query: 518 IVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 551
           +V T     TE+EI ++V+  V  YK++  V FV+ IPKSPSGK++R+ ++ K+
Sbjct: 489 VVRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRAKI 537

BLAST of Csor.00g168780 vs. ExPASy Swiss-Prot
Match: A0A2H5AIX5 (4-coumarate-CoA ligase 1 OS=Narcissus pseudonarcissus OX=39639 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 1.6e-103
Identity = 210/536 (39.18%), Postives = 320/536 (59.70%), Query Frame = 0

Query: 37  DEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFA 96
           ++E IFRS+LP++ VPD + L  +  QN   ++D    ++  + K  TY EV   + R  
Sbjct: 7   EKETIFRSKLPDIYVPDHLPLHSYCFQNLHQFSDRPCLIDGFTNKTLTYAEVELASKRVG 66

Query: 97  KALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAK 156
             L  L L++G VV+++LPN  E+ +  +G    G + +  NP    +EI KQ   A AK
Sbjct: 67  AGLHRLGLRQGHVVMLLLPNSIEFVLSFIGASLLGAMSTTANPFYTSAEIHKQAAAAGAK 126

Query: 157 LVVTNSSSFEKVKELK-LPVIVVGEELI---EGSMNWHQLLEAADRAGNNFVKEDIKQTD 216
           ++VT S    K++ L+ +  IVV ++ +   E  M++ + LE+ D A     + D+   D
Sbjct: 127 IIVTESCHVSKLQGLEGISRIVVIDDAVRVPENVMHFSE-LESTDEA--ELPRIDVHPDD 186

Query: 217 LCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEM--VGKVTTLGLIPFFHIYGIT 276
           + ALP+SSGTTG+ KGVMLTH  LV ++   + G    +        L ++P FHIY + 
Sbjct: 187 VVALPYSSGTTGLPKGVMLTHNGLVTSVAQQVDGENPNLHFSEDDVLLCVLPLFHIYSLN 246

Query: 277 GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGL 336
            +    LR    +V+M +F++   +  +    VT AP VPPI++ +VKN  V  +DLS +
Sbjct: 247 SVLLCGLRAGAAIVLMRKFEIVRLMELVEKYRVTIAPFVPPIVVEMVKNEAVDRYDLSSI 306

Query: 337 KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTE-----HSCIT-------LNYGSI 396
           ++  +M+ AAP+  EL+    +K P   + + YG+TE       C+        +  GS 
Sbjct: 307 RV--VMSGAAPMGKELENKLREKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPFEVKSGSC 366

Query: 397 G----KDNLTLQRKTRGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGD 456
           G       L +     G SL +N PGEIC+R   +M+GY NN E T +TID++GW+HTGD
Sbjct: 367 GTVVRNAELKIIDPETGFSLSRNQPGEICIRGNQIMKGYLNNPEATKQTIDEEGWLHTGD 426

Query: 457 IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA 516
           IG++DDD ++FIVDR+KELIKYKGFQVAPAELEA+L+THP++ DAAVV + D   GE+P 
Sbjct: 427 IGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEAMLITHPNMADAAVVSIKDDSCGELPV 486

Query: 517 ASIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 551
           A IV +  ++ TEDEI KY++  V  YK++  V F+E IPK+PSGK++R+ ++ ++
Sbjct: 487 AFIVRSNGSEITEDEIKKYISKQVVFYKRIHRVFFIEAIPKAPSGKILRKELRARL 537

BLAST of Csor.00g168780 vs. NCBI nr
Match: KAG6573594.1 (4-coumarate--CoA ligase-like 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1087 bits (2812), Expect = 0.0
Identity = 560/560 (100.00%), Postives = 560/560 (100.00%), Query Frame = 0

Query: 1   MIQAFSAPPLRRGRRPSMSAANNNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEF 60
           MIQAFSAPPLRRGRRPSMSAANNNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEF
Sbjct: 1   MIQAFSAPPLRRGRRPSMSAANNNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEF 60

Query: 61  VLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEY 120
           VLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEY
Sbjct: 61  VLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEY 120

Query: 121 AIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGE 180
           AIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGE
Sbjct: 121 AIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGE 180

Query: 181 ELIEGSMNWHQLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANL 240
           ELIEGSMNWHQLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANL
Sbjct: 181 ELIEGSMNWHQLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANL 240

Query: 241 CSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALIT 300
           CSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALIT
Sbjct: 241 CSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALIT 300

Query: 301 QEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQ 360
           QEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQ
Sbjct: 301 QEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQ 360

Query: 361 EAYGLTEHSCITLNYGSIGKDNLTLQRKTRGRSLPKNTPGEICVRSQCVMQGYYNNEEET 420
           EAYGLTEHSCITLNYGSIGKDNLTLQRKTRGRSLPKNTPGEICVRSQCVMQGYYNNEEET
Sbjct: 361 EAYGLTEHSCITLNYGSIGKDNLTLQRKTRGRSLPKNTPGEICVRSQCVMQGYYNNEEET 420

Query: 421 SRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAA 480
           SRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAA
Sbjct: 421 SRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAA 480

Query: 481 VVPLPDKEAGEIPAASIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGK 540
           VVPLPDKEAGEIPAASIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGK
Sbjct: 481 VVPLPDKEAGEIPAASIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGK 540

Query: 541 VMRRLIKEKMIEKIRAETAS 560
           VMRRLIKEKMIEKIRAETAS
Sbjct: 541 VMRRLIKEKMIEKIRAETAS 560

BLAST of Csor.00g168780 vs. NCBI nr
Match: KAG7012722.1 (4-coumarate--CoA ligase-like 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1066 bits (2757), Expect = 0.0
Identity = 554/576 (96.18%), Postives = 556/576 (96.53%), Query Frame = 0

Query: 1   MIQAFSAPPLRRGRRPSMSAANNNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEF 60
           MIQAFSAPPLRRGRRPSMSAANNNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEF
Sbjct: 31  MIQAFSAPPLRRGRRPSMSAANNNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEF 90

Query: 61  VLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEY 120
           VLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEY
Sbjct: 91  VLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEY 150

Query: 121 AIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGE 180
           AIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGE
Sbjct: 151 AIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGE 210

Query: 181 ELIEGSMNWHQLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANL 240
           ELIEGSMNWHQLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANL
Sbjct: 211 ELIEGSMNWHQLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANL 270

Query: 241 CSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALIT 300
           CSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALIT
Sbjct: 271 CSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALIT 330

Query: 301 QEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQ 360
           QEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQ
Sbjct: 331 QEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQ 390

Query: 361 EAYGLTEHSCITLNYGSIGKDNLTLQRKT----------------RGRSLPKNTPGEICV 420
           EAYGLTEHSCITLNYGSIGKDNL  ++ T                 GRSLPKNTPGEICV
Sbjct: 391 EAYGLTEHSCITLNYGSIGKDNLAAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICV 450

Query: 421 RSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA 480
           RSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA
Sbjct: 451 RSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA 510

Query: 481 ELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMTPNAKETEDEIIKYVASNVAHYKKV 540
           ELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMTPNAKETEDEIIKYVASNVAHYKKV
Sbjct: 511 ELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMTPNAKETEDEIIKYVASNVAHYKKV 570

Query: 541 RLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAETAS 560
           RLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAETAS
Sbjct: 571 RLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAETAS 606

BLAST of Csor.00g168780 vs. NCBI nr
Match: XP_022945246.1 (4-coumarate--CoA ligase-like 1 [Cucurbita moschata])

HSP 1 Score: 1058 bits (2737), Expect = 0.0
Identity = 550/576 (95.49%), Postives = 553/576 (96.01%), Query Frame = 0

Query: 1   MIQAFSAPPLRRGRRPSMSAANNNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEF 60
           MIQAFSAPPLRRGRRPSMSAANNNSAMATCIRD VEDEEHIFRSQLPEVQVPDDITLPEF
Sbjct: 31  MIQAFSAPPLRRGRRPSMSAANNNSAMATCIRDLVEDEEHIFRSQLPEVQVPDDITLPEF 90

Query: 61  VLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEY 120
           VLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEY
Sbjct: 91  VLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEY 150

Query: 121 AIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGE 180
           AIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGE
Sbjct: 151 AIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGE 210

Query: 181 ELIEGSMNWHQLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANL 240
           ELIEGSMNWH+LLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANL
Sbjct: 211 ELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANL 270

Query: 241 CSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALIT 300
           CSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALIT
Sbjct: 271 CSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALIT 330

Query: 301 QEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQ 360
           QEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQ
Sbjct: 331 QEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQ 390

Query: 361 EAYGLTEHSCITLNYGSIGKDNLTLQRKT----------------RGRSLPKNTPGEICV 420
           EAYGLTEHSCITLNYGSIGKDNL  ++ T                 GRSLPKNTPGEICV
Sbjct: 391 EAYGLTEHSCITLNYGSIGKDNLAAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICV 450

Query: 421 RSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA 480
           RSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA
Sbjct: 451 RSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA 510

Query: 481 ELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMTPNAKETEDEIIKYVASNVAHYKKV 540
           ELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVM PNAKETEDEIIKYVASNVAHYKKV
Sbjct: 511 ELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMAPNAKETEDEIIKYVASNVAHYKKV 570

Query: 541 RLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAETAS 560
           RLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAE AS
Sbjct: 571 RLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAEAAS 606

BLAST of Csor.00g168780 vs. NCBI nr
Match: XP_023541569.1 (4-coumarate--CoA ligase-like 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1053 bits (2723), Expect = 0.0
Identity = 548/576 (95.14%), Postives = 551/576 (95.66%), Query Frame = 0

Query: 1   MIQAFSAPPLRRGRRPSMSAANNNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEF 60
           MIQAFSAPPLRRGRR  MSAA NNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEF
Sbjct: 31  MIQAFSAPPLRRGRRSLMSAATNNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEF 90

Query: 61  VLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEY 120
           VLQNAESYADNVAFVEAVSG AYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEY
Sbjct: 91  VLQNAESYADNVAFVEAVSGNAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEY 150

Query: 121 AIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGE 180
           AIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGE
Sbjct: 151 AIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGE 210

Query: 181 ELIEGSMNWHQLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANL 240
           ELIEGSMNWH+LLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANL
Sbjct: 211 ELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANL 270

Query: 241 CSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALIT 300
           CSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALIT
Sbjct: 271 CSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALIT 330

Query: 301 QEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQ 360
           QEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQ
Sbjct: 331 QEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQ 390

Query: 361 EAYGLTEHSCITLNYGSIGKDNLTLQRKT----------------RGRSLPKNTPGEICV 420
           EAYGLTEHSCITLNYGSIGKDNL  ++ T                 GRSLPKNTPGEICV
Sbjct: 391 EAYGLTEHSCITLNYGSIGKDNLAAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICV 450

Query: 421 RSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA 480
           RSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA
Sbjct: 451 RSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA 510

Query: 481 ELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMTPNAKETEDEIIKYVASNVAHYKKV 540
           ELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVM PNAKETEDEIIKYVASNVAHYKKV
Sbjct: 511 ELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMAPNAKETEDEIIKYVASNVAHYKKV 570

Query: 541 RLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAETAS 560
           RLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAETAS
Sbjct: 571 RLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAETAS 606

BLAST of Csor.00g168780 vs. NCBI nr
Match: XP_022966799.1 (4-coumarate--CoA ligase-like 1 [Cucurbita maxima])

HSP 1 Score: 1048 bits (2710), Expect = 0.0
Identity = 543/576 (94.27%), Postives = 550/576 (95.49%), Query Frame = 0

Query: 1   MIQAFSAPPLRRGRRPSMSAANNNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEF 60
           MIQAFSAPPLRRGRR SMSAANNNS MATCIRDS EDEEHIFRSQLPEVQVPDDITLPEF
Sbjct: 31  MIQAFSAPPLRRGRRSSMSAANNNSTMATCIRDSAEDEEHIFRSQLPEVQVPDDITLPEF 90

Query: 61  VLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEY 120
           VLQNAE YADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQ+VIVVLPNVAEY
Sbjct: 91  VLQNAELYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQIVIVVLPNVAEY 150

Query: 121 AIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGE 180
           AIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGE
Sbjct: 151 AIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGE 210

Query: 181 ELIEGSMNWHQLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANL 240
           ELIEGSMNWH+LLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANL
Sbjct: 211 ELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANL 270

Query: 241 CSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALIT 300
           CSTLSG+PQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALIT
Sbjct: 271 CSTLSGIPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALIT 330

Query: 301 QEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQ 360
           QEVTFAPIVPPIILALVKNPIVKEFDLS LKLQAIMTAAAPLAPELQTSFEKKFPGVDVQ
Sbjct: 331 QEVTFAPIVPPIILALVKNPIVKEFDLSSLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQ 390

Query: 361 EAYGLTEHSCITLNYGSIGKDNLTLQRKT----------------RGRSLPKNTPGEICV 420
           EAYGLTEHSCITLNYG+IGKDNL  ++ T                 GRSLPKNTPGEICV
Sbjct: 391 EAYGLTEHSCITLNYGNIGKDNLAAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICV 450

Query: 421 RSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA 480
           RSQCVMQGYYNNEEETSRTID+KGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA
Sbjct: 451 RSQCVMQGYYNNEEETSRTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA 510

Query: 481 ELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMTPNAKETEDEIIKYVASNVAHYKKV 540
           ELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVM PNAKETEDEIIKYVASNVAHYKKV
Sbjct: 511 ELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMAPNAKETEDEIIKYVASNVAHYKKV 570

Query: 541 RLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAETAS 560
           RLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAETAS
Sbjct: 571 RLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAETAS 606

BLAST of Csor.00g168780 vs. ExPASy TrEMBL
Match: A0A6J1G0B4 (4-coumarate--CoA ligase-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111449548 PE=4 SV=1)

HSP 1 Score: 1058 bits (2737), Expect = 0.0
Identity = 550/576 (95.49%), Postives = 553/576 (96.01%), Query Frame = 0

Query: 1   MIQAFSAPPLRRGRRPSMSAANNNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEF 60
           MIQAFSAPPLRRGRRPSMSAANNNSAMATCIRD VEDEEHIFRSQLPEVQVPDDITLPEF
Sbjct: 31  MIQAFSAPPLRRGRRPSMSAANNNSAMATCIRDLVEDEEHIFRSQLPEVQVPDDITLPEF 90

Query: 61  VLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEY 120
           VLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEY
Sbjct: 91  VLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEY 150

Query: 121 AIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGE 180
           AIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGE
Sbjct: 151 AIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGE 210

Query: 181 ELIEGSMNWHQLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANL 240
           ELIEGSMNWH+LLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANL
Sbjct: 211 ELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANL 270

Query: 241 CSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALIT 300
           CSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALIT
Sbjct: 271 CSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALIT 330

Query: 301 QEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQ 360
           QEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQ
Sbjct: 331 QEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQ 390

Query: 361 EAYGLTEHSCITLNYGSIGKDNLTLQRKT----------------RGRSLPKNTPGEICV 420
           EAYGLTEHSCITLNYGSIGKDNL  ++ T                 GRSLPKNTPGEICV
Sbjct: 391 EAYGLTEHSCITLNYGSIGKDNLAAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICV 450

Query: 421 RSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA 480
           RSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA
Sbjct: 451 RSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA 510

Query: 481 ELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMTPNAKETEDEIIKYVASNVAHYKKV 540
           ELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVM PNAKETEDEIIKYVASNVAHYKKV
Sbjct: 511 ELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMAPNAKETEDEIIKYVASNVAHYKKV 570

Query: 541 RLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAETAS 560
           RLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAE AS
Sbjct: 571 RLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAEAAS 606

BLAST of Csor.00g168780 vs. ExPASy TrEMBL
Match: A0A6J1HUV5 (4-coumarate--CoA ligase-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111466396 PE=4 SV=1)

HSP 1 Score: 1048 bits (2710), Expect = 0.0
Identity = 543/576 (94.27%), Postives = 550/576 (95.49%), Query Frame = 0

Query: 1   MIQAFSAPPLRRGRRPSMSAANNNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEF 60
           MIQAFSAPPLRRGRR SMSAANNNS MATCIRDS EDEEHIFRSQLPEVQVPDDITLPEF
Sbjct: 31  MIQAFSAPPLRRGRRSSMSAANNNSTMATCIRDSAEDEEHIFRSQLPEVQVPDDITLPEF 90

Query: 61  VLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEY 120
           VLQNAE YADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQ+VIVVLPNVAEY
Sbjct: 91  VLQNAELYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQIVIVVLPNVAEY 150

Query: 121 AIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGE 180
           AIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGE
Sbjct: 151 AIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGE 210

Query: 181 ELIEGSMNWHQLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANL 240
           ELIEGSMNWH+LLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANL
Sbjct: 211 ELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANL 270

Query: 241 CSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALIT 300
           CSTLSG+PQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALIT
Sbjct: 271 CSTLSGIPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALIT 330

Query: 301 QEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQ 360
           QEVTFAPIVPPIILALVKNPIVKEFDLS LKLQAIMTAAAPLAPELQTSFEKKFPGVDVQ
Sbjct: 331 QEVTFAPIVPPIILALVKNPIVKEFDLSSLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQ 390

Query: 361 EAYGLTEHSCITLNYGSIGKDNLTLQRKT----------------RGRSLPKNTPGEICV 420
           EAYGLTEHSCITLNYG+IGKDNL  ++ T                 GRSLPKNTPGEICV
Sbjct: 391 EAYGLTEHSCITLNYGNIGKDNLAAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICV 450

Query: 421 RSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA 480
           RSQCVMQGYYNNEEETSRTID+KGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA
Sbjct: 451 RSQCVMQGYYNNEEETSRTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA 510

Query: 481 ELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMTPNAKETEDEIIKYVASNVAHYKKV 540
           ELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVM PNAKETEDEIIKYVASNVAHYKKV
Sbjct: 511 ELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMAPNAKETEDEIIKYVASNVAHYKKV 570

Query: 541 RLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAETAS 560
           RLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAETAS
Sbjct: 571 RLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAETAS 606

BLAST of Csor.00g168780 vs. ExPASy TrEMBL
Match: A0A6J1DG05 (4-coumarate--CoA ligase-like 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111019748 PE=4 SV=1)

HSP 1 Score: 962 bits (2487), Expect = 0.0
Identity = 496/561 (88.41%), Postives = 525/561 (93.58%), Query Frame = 0

Query: 16  PSMSAANNNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFV 75
           PS     N SAM T IRD VEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYA+NVAFV
Sbjct: 49  PSSMPVANISAMGTLIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFV 108

Query: 76  EAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFS 135
           EAVSGKAYTYREVVRDT RFAKALSSLRL+KGQVVIVVLPNVAEYAIVALGIMAAGGVFS
Sbjct: 109 EAVSGKAYTYREVVRDTKRFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFS 168

Query: 136 GVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHQLLEA 195
           GVNPAAHISEIKKQVE ADAKLVVTNS +FEKVKELKLPVIV+GEELIEGSMNWH+LLEA
Sbjct: 169 GVNPAAHISEIKKQVEAADAKLVVTNSPNFEKVKELKLPVIVLGEELIEGSMNWHKLLEA 228

Query: 196 ADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKV 255
           ADRAGNN VKEDIKQ+DLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVP+E  GKV
Sbjct: 229 ADRAGNNHVKEDIKQSDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPRETEGKV 288

Query: 256 TTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILA 315
           TTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILA
Sbjct: 289 TTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILA 348

Query: 316 LVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNY 375
           LVKNPIV+EFDLS LKLQAIMTAAAPLAPE+QT+FEKKFPGVDVQEAYGLTEHSCITLNY
Sbjct: 349 LVKNPIVEEFDLSSLKLQAIMTAAAPLAPEIQTAFEKKFPGVDVQEAYGLTEHSCITLNY 408

Query: 376 GSIGKDNLTLQRKT----------------RGRSLPKNTPGEICVRSQCVMQGYYNNEEE 435
           GSIGK+NLT ++ T                 GRSLPKNTPGEICVRSQCVMQGYYNNEEE
Sbjct: 409 GSIGKENLTAKKNTVGRILPNLEVKFIDPESGRSLPKNTPGEICVRSQCVMQGYYNNEEE 468

Query: 436 TSRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDA 495
           TSRTID++GW+HTGDIGYIDD+GDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDA
Sbjct: 469 TSRTIDNRGWLHTGDIGYIDDNGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDA 528

Query: 496 AVVPLPDKEAGEIPAASIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSG 555
           AVVPLPD+EAGEIPAAS+V+ PN+KETE+EII+YVASNVA+YKKVR+VHFV++IPKSPSG
Sbjct: 529 AVVPLPDEEAGEIPAASVVIAPNSKETEEEIIQYVASNVAYYKKVRVVHFVDSIPKSPSG 588

Query: 556 KVMRRLIKEKMIEKIRAETAS 560
           KVMRRLIKEKMI+KIRA+TAS
Sbjct: 589 KVMRRLIKEKMIDKIRAKTAS 609

BLAST of Csor.00g168780 vs. ExPASy TrEMBL
Match: A0A6J1DEE1 (4-coumarate--CoA ligase-like 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019748 PE=4 SV=1)

HSP 1 Score: 960 bits (2481), Expect = 0.0
Identity = 494/554 (89.17%), Postives = 523/554 (94.40%), Query Frame = 0

Query: 23  NNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKA 82
           N SAM T IRD VEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYA+NVAFVEAVSGKA
Sbjct: 5   NISAMGTLIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSGKA 64

Query: 83  YTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAH 142
           YTYREVVRDT RFAKALSSLRL+KGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAH
Sbjct: 65  YTYREVVRDTKRFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAH 124

Query: 143 ISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHQLLEAADRAGNN 202
           ISEIKKQVE ADAKLVVTNS +FEKVKELKLPVIV+GEELIEGSMNWH+LLEAADRAGNN
Sbjct: 125 ISEIKKQVEAADAKLVVTNSPNFEKVKELKLPVIVLGEELIEGSMNWHKLLEAADRAGNN 184

Query: 203 FVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIP 262
            VKEDIKQ+DLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVP+E  GKVTTLGLIP
Sbjct: 185 HVKEDIKQSDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPRETEGKVTTLGLIP 244

Query: 263 FFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIV 322
           FFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIV
Sbjct: 245 FFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIV 304

Query: 323 KEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDN 382
           +EFDLS LKLQAIMTAAAPLAPE+QT+FEKKFPGVDVQEAYGLTEHSCITLNYGSIGK+N
Sbjct: 305 EEFDLSSLKLQAIMTAAAPLAPEIQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKEN 364

Query: 383 LTLQRKT----------------RGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDD 442
           LT ++ T                 GRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTID+
Sbjct: 365 LTAKKNTVGRILPNLEVKFIDPESGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDN 424

Query: 443 KGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD 502
           +GW+HTGDIGYIDD+GDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD
Sbjct: 425 RGWLHTGDIGYIDDNGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD 484

Query: 503 KEAGEIPAASIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLI 560
           +EAGEIPAAS+V+ PN+KETE+EII+YVASNVA+YKKVR+VHFV++IPKSPSGKVMRRLI
Sbjct: 485 EEAGEIPAASVVIAPNSKETEEEIIQYVASNVAYYKKVRVVHFVDSIPKSPSGKVMRRLI 544

BLAST of Csor.00g168780 vs. ExPASy TrEMBL
Match: A0A5D3CV41 (4-coumarate--CoA ligase-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G002330 PE=4 SV=1)

HSP 1 Score: 947 bits (2448), Expect = 0.0
Identity = 486/576 (84.38%), Postives = 528/576 (91.67%), Query Frame = 0

Query: 1   MIQAFSAPPLRRGRRPSMSAANNNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEF 60
           MIQ FS   L RG+   + A N  SAM T  +DSV+DE+HIFRSQLPEVQVP DITLPEF
Sbjct: 1   MIQPFSTLLLSRGKLIHVEAENYYSAMTTRNQDSVQDEKHIFRSQLPEVQVPCDITLPEF 60

Query: 61  VLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEY 120
           VLQNAESYA+NVAFVEA+SGKAYTYREV+RDT+RF+KAL SLRLKKG VV+VVLPNVAEY
Sbjct: 61  VLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALHSLRLKKGHVVLVVLPNVAEY 120

Query: 121 AIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGE 180
           AIVALGIM AGGVFSGVNPAAHISEIKKQVEVA+AKLVVTN+++FEKV+ELKLPVI++ E
Sbjct: 121 AIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAANFEKVRELKLPVILLEE 180

Query: 181 ELIEGSMNWHQLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANL 240
           EL+EG+MNWH+LLEAADRAGNNFVKE+IKQTDLCALPFSSGTTGVSKGVMLTHRNLVAN+
Sbjct: 181 ELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANM 240

Query: 241 CSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALIT 300
           CSTLSGVPQEM GKVTTLGLIPFFHIYGITGICC+TLRNKGKVVVMGRFDLRTFINALIT
Sbjct: 241 CSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVMGRFDLRTFINALIT 300

Query: 301 QEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQ 360
            E+TFAPIVPPIILALVKNPIV+EFDLS LKLQAIMTAAAPLAPELQT+FE+KFPGVDVQ
Sbjct: 301 HEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQ 360

Query: 361 EAYGLTEHSCITLNYGSIGKDNLTLQRKT----------------RGRSLPKNTPGEICV 420
           EAYGLTEH CITLNYG+IGK+NL+ ++ T                 GRSLPKNTPGEICV
Sbjct: 361 EAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICV 420

Query: 421 RSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA 480
           RSQCVMQGYY NEEETSRTID KGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA
Sbjct: 421 RSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA 480

Query: 481 ELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMTPNAKETEDEIIKYVASNVAHYKKV 540
           ELEAILLTHPSIEDAAVVPLPD+EAGEIPAAS+VM PN+ ETE+EIIK+VASNVAHYKKV
Sbjct: 481 ELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEEEIIKHVASNVAHYKKV 540

Query: 541 RLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAETAS 560
           RLVHFV+TIPKSPSGKVMRRLIKEKMIEKIRA+ ++
Sbjct: 541 RLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESA 576

BLAST of Csor.00g168780 vs. TAIR 10
Match: AT1G62940.1 (acyl-CoA synthetase 5 )

HSP 1 Score: 742.3 bits (1915), Expect = 2.9e-214
Identity = 372/532 (69.92%), Postives = 441/532 (82.89%), Query Frame = 0

Query: 36  EDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRF 95
           ED E+IFRS  P V +PD +TLPEFVLQ  E Y +NVAFVEAV+GKA TY +VVRDT R 
Sbjct: 7   EDNEYIFRSLYPSVPIPDKLTLPEFVLQGVEEYTENVAFVEAVTGKAVTYGDVVRDTKRL 66

Query: 96  AKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADA 155
           AKAL+SL L+KGQV++VVLPNVAEY I+ALGIM+AGGVFSG NP A +SEIKKQVE + A
Sbjct: 67  AKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGA 126

Query: 156 KLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHQLLEAADRAGNNFVKEDIKQTDLCA 215
           + ++T+++++EKVK L LPVIV+GEE IEG++NW  LLEA D+ G+    E+I QTDLCA
Sbjct: 127 RGIITDATNYEKVKSLGLPVIVLGEEKIEGAVNWKDLLEAGDKCGDT-DNEEILQTDLCA 186

Query: 216 LPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCA 275
           LPFSSGTTG+ KGVMLTHRNL+ANLCSTL GV  EM+G++ TLGLIPFFHIYGI GICCA
Sbjct: 187 LPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVRSEMIGQIVTLGLIPFFHIYGIVGICCA 246

Query: 276 TLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAI 335
           T++NKGKVV M R+DLR F+NALI  EV+FAPIVPPIIL LVKNPIV EFDLS LKLQ++
Sbjct: 247 TMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLQSV 306

Query: 336 MTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLTLQRKT------ 395
           MTAAAPLAPEL T+FE KFP V VQEAYGLTEHSCITL +G   K     +R +      
Sbjct: 307 MTAAAPLAPELLTAFEAKFPNVQVQEAYGLTEHSCITLTHGDPEKGQGIAKRNSVGFILP 366

Query: 396 ----------RGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYID 455
                      GRSLPKNT GE+CVRSQCVMQGY+ N+EET +TID++GW+HTGDIGYID
Sbjct: 367 NLEVKFIDPDTGRSLPKNTSGELCVRSQCVMQGYFMNKEETDKTIDEQGWLHTGDIGYID 426

Query: 456 DDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVM 515
           DDGD+FIVDRIKELIKYKGFQVAPAELEAILLTHPS+ED AVVPLPD+EAGEIPAA +V+
Sbjct: 427 DDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDEEAGEIPAACVVI 486

Query: 516 TPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMI 552
            P A E E++I+ +VA+NVAHYKKVR VHFV++IPKS SGK+MRRL+++K++
Sbjct: 487 NPKATEKEEDILNFVAANVAHYKKVRAVHFVDSIPKSLSGKIMRRLLRDKIL 537

BLAST of Csor.00g168780 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 363.2 bits (931), Expect = 3.7e-100
Identity = 192/532 (36.09%), Postives = 319/532 (59.96%), Query Frame = 0

Query: 41  IFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALS 100
           IFRS+LP++ +P+ + L +++ +N   +A     +   +G+ YTY +V   + + A  L 
Sbjct: 22  IFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLAAGLH 81

Query: 101 SLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVT 160
           +L +K+  VV+++LPN  E  +  L     G + +  NP    +EI KQ + + AKL+VT
Sbjct: 82  NLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAKLIVT 141

Query: 161 NSSSFEKVKELK----LPVIVVGEELIEGSMNWHQLLEAADRAGNNFVKEDIKQTDLCAL 220
            S   +K+K L+    L V    + + E  + + +L ++ +   ++ + E I   D+ AL
Sbjct: 142 QSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDS-IPEKISPEDVVAL 201

Query: 221 PFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMV--GKVTTLGLIPFFHIYGITGICC 280
           PFSSGTTG+ KGVMLTH+ LV ++   + G    +        L ++P FHIY +  I  
Sbjct: 202 PFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALNSIML 261

Query: 281 ATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQA 340
            +LR    +++M +F++   +  +   +VT A +VPPI+LA+ K+P  +++DLS +++  
Sbjct: 262 CSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVRM-- 321

Query: 341 IMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHS---CITLNYGS-------------I 400
           + + AAPL  EL+ +   KFP   + + YG+TE      ++L +               +
Sbjct: 322 VKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVV 381

Query: 401 GKDNLTLQRKTRGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYI 460
               + +     G SLP+N PGEIC+R   +M+GY N+   T+ TID  GW+HTGD+G+I
Sbjct: 382 RNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGFI 441

Query: 461 DDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIV 520
           DDD ++FIVDR+KELIKYKGFQVAPAELE++L+ HP I D AVV + +++AGE+P A +V
Sbjct: 442 DDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFVV 501

Query: 521 MTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 551
            + ++  +EDEI ++V+  V  YK++  V F ++IPK+PSGK++R+ ++ ++
Sbjct: 502 RSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARL 550

BLAST of Csor.00g168780 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 361.3 bits (926), Expect = 1.4e-99
Identity = 204/556 (36.69%), Postives = 320/556 (57.55%), Query Frame = 0

Query: 16  PSMSAANNNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFV 75
           P +    + S ++  +         IFRS+LP++ +P+ + L  +  +   S +D    +
Sbjct: 9   PQIHKPTDTSVVSDDVLPHSPPTPRIFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLI 68

Query: 76  EAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFS 135
              +GK+YTY E      R A  L  L ++KG V++++L N AE+    +G    G V +
Sbjct: 69  VGSTGKSYTYGETHLICRRVASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVST 128

Query: 136 GVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKEL--KLPVIVVGEELIEGSMNWHQLL 195
             NP     E+ KQ++ + AKL++T+S   +K+K L   L +I   E   E  + +  L+
Sbjct: 129 TANPFYTSQELYKQLKSSGAKLIITHSQYVDKLKNLGENLTLITTDEPTPENCLPFSTLI 188

Query: 196 EAADRAGNNFVKE-DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMV 255
              D   N F +  DI   D  ALPFSSGTTG+ KGV+LTH++L+ ++   + G    + 
Sbjct: 189 --TDDETNPFQETVDIGGDDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLY 248

Query: 256 GKV--TTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVP 315
            K     L ++P FHIY +  +   +LR+   V++M +F++   ++ +    VT A +VP
Sbjct: 249 LKSNDVILCVLPLFHIYSLNSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVP 308

Query: 316 PIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSC 375
           P+++AL KNP V  +DLS ++   +++ AAPL  ELQ S  ++ P   + + YG+TE   
Sbjct: 309 PLVIALAKNPTVNSYDLSSVRF--VLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGP 368

Query: 376 ITLNYGSIGKDNLTLQRKTRGR----------------SLPKNTPGEICVRSQCVMQGYY 435
           +        K+ +  +  + G                 SL  N PGEIC+R Q +M+ Y 
Sbjct: 369 VLSMSLGFAKEPIPTKSGSCGTVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYL 428

Query: 436 NNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHP 495
           N+ E TS TID++GW+HTGDIGY+D+D ++FIVDR+KE+IK+KGFQV PAELE++L+ H 
Sbjct: 429 NDPEATSATIDEEGWLHTGDIGYVDEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHH 488

Query: 496 SIEDAAVVPLPDKEAGEIPAASIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIP 551
           SI DAAVVP  D+ AGE+P A +V +     TE+++ +YVA  V  YK++  V FV +IP
Sbjct: 489 SIADAAVVPQNDEVAGEVPVAFVVRSNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIP 548

BLAST of Csor.00g168780 vs. TAIR 10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 359.0 bits (920), Expect = 6.9e-99
Identity = 202/542 (37.27%), Postives = 327/542 (60.33%), Query Frame = 0

Query: 41  IFRSQLPEVQVPDD--ITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKA 100
           I+RS  P + +P D   +L  F+ +N+ SY   +A  ++ +G + T+ ++    +R A  
Sbjct: 11  IYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARLAHG 70

Query: 101 LSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLV 160
              L ++K  VV++  PN  ++ +  L + A GGVF+  NP   ++E+ KQ++ ++ K++
Sbjct: 71  FHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKII 130

Query: 161 VTNSSSFEKVKELKLPVIVVGE----ELIEGS----MNWHQLLEAADRAGNNFVKEDIKQ 220
           ++ +  F+K+K   LPV+++G     E+  GS    +++  ++E ++   + +   +IKQ
Sbjct: 131 ISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKILSFDNVMELSEPV-SEYPFVEIKQ 190

Query: 221 TDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKV--TTLGLIPFFHIYG 280
           +D  AL +SSGTTG SKGV LTH N +A   S +  + Q+++G+     L  +P FH++G
Sbjct: 191 SDTAALLYSSGTTGTSKGVELTHGNFIA--ASLMVTMDQDLMGEYHGVFLCFLPMFHVFG 250

Query: 281 ITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLS 340
           +  I  + L+    +V M RF+L   +  +    VT   +VPP+ LAL K  IVK+FDLS
Sbjct: 251 LAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSIVKKFDLS 310

Query: 341 GLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEH-SCITLNYGSIGKDN----- 400
            LK   I + AAPL  +L     +  P V + + YG+TE    +++    +GK N     
Sbjct: 311 SLKY--IGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLGKRNSGSAG 370

Query: 401 -------LTLQRKTRGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDI 460
                    +     G+S P N  GEI VR   +M+GY NN + T  TID K W+HTGD+
Sbjct: 371 MLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWVHTGDL 430

Query: 461 GYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAA 520
           GY ++DG++++VDRIKELIKYKGFQVAPAELE +L++HP I DA V+P PD+EAGE+P A
Sbjct: 431 GYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAGEVPIA 490

Query: 521 SIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKIR 558
            +V +PN+  TE +I K++A  VA YK++R V F+  +PKS +GK++RR    ++++++R
Sbjct: 491 FVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRR----ELVQQVR 543

BLAST of Csor.00g168780 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 354.8 bits (909), Expect = 1.3e-97
Identity = 188/536 (35.07%), Postives = 313/536 (58.40%), Query Frame = 0

Query: 41  IFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALS 100
           IFRS+LP++ +P+ ++L +++ QN   +A     +   +G  YTY +V   + + A    
Sbjct: 24  IFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIAANFH 83

Query: 101 SLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVT 160
            L + +  VV+++LPN  E+ +  L     G   +  NP    +EI KQ + ++ KL++T
Sbjct: 84  KLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKLIIT 143

Query: 161 NSSSFEKVKELK----LPVIVVGEE----LIEGSMNWHQLLEAADRAGNNFVKEDIKQTD 220
            +   +K+K L+    + ++ + +     + EG + + +L ++   A       +I   D
Sbjct: 144 EARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEISPDD 203

Query: 221 LCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMV--GKVTTLGLIPFFHIYGIT 280
           + ALP+SSGTTG+ KGVMLTH+ LV ++   + G    +        L ++P FHIY + 
Sbjct: 204 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHIYALN 263

Query: 281 GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGL 340
            I    LR    +++M +F++   +  +   +VT AP+VPPI+LA+ K+   +++DLS +
Sbjct: 264 SIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYDLSSI 323

Query: 341 KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHS---CITLNYGS---------- 400
           ++  + + AAPL  EL+ +   KFP   + + YG+TE      ++L +            
Sbjct: 324 RV--VKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGAC 383

Query: 401 ---IGKDNLTLQRKTRGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGD 460
              +    + +     G SL +N PGEIC+R   +M+GY NN   T+ TID  GW+HTGD
Sbjct: 384 GTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHTGD 443

Query: 461 IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA 520
           IG IDDD ++FIVDR+KELIKYKGFQVAPAELEA+L+ HP I D AVV + ++ AGE+P 
Sbjct: 444 IGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEVPV 503

Query: 521 ASIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 551
           A +V + +++ +ED++ ++V+  V  YK++  V F E+IPK+PSGK++R+ ++ K+
Sbjct: 504 AFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKL 557

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LQ124.1e-21369.924-coumarate--CoA ligase-like 1 OS=Arabidopsis thaliana OX=3702 GN=4CLL1 PE=1 SV=... [more]
Q7XXL22.3e-18761.974-coumarate--CoA ligase-like 9 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL9... [more]
P149124.1e-10437.834-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1[more]
P149137.0e-10438.014-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1[more]
A0A2H5AIX51.6e-10339.184-coumarate-CoA ligase 1 OS=Narcissus pseudonarcissus OX=39639 GN=4CL1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAG6573594.10.0100.004-coumarate--CoA ligase-like 1, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7012722.10.096.184-coumarate--CoA ligase-like 1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022945246.10.095.494-coumarate--CoA ligase-like 1 [Cucurbita moschata][more]
XP_023541569.10.095.144-coumarate--CoA ligase-like 1 [Cucurbita pepo subsp. pepo][more]
XP_022966799.10.094.274-coumarate--CoA ligase-like 1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1G0B40.095.494-coumarate--CoA ligase-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111449548 PE=... [more]
A0A6J1HUV50.094.274-coumarate--CoA ligase-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111466396 PE=4 ... [more]
A0A6J1DG050.088.414-coumarate--CoA ligase-like 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A6J1DEE10.089.174-coumarate--CoA ligase-like 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A5D3CV410.084.384-coumarate--CoA ligase-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
Match NameE-valueIdentityDescription
AT1G62940.12.9e-21469.92acyl-CoA synthetase 5 [more]
AT3G21240.13.7e-10036.094-coumarate:CoA ligase 2 [more]
AT1G65060.11.4e-9936.694-coumarate:CoA ligase 3 [more]
AT4G05160.16.9e-9937.27AMP-dependent synthetase and ligase family protein [more]
AT1G51680.11.3e-9735.074-coumarate:CoA ligase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 39..451
e-value: 1.3E-119
score: 401.7
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 63..456
e-value: 5.8E-86
score: 288.7
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 465..540
e-value: 3.3E-16
score: 60.0
NoneNo IPR availableGENE3D3.30.300.30coord: 452..554
e-value: 1.3E-30
score: 108.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..23
NoneNo IPR availablePANTHERPTHR24096:SF3894-COUMARATE--COA LIGASE-LIKE 1coord: 33..553
NoneNo IPR availablePANTHERPTHR24096LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 33..553
NoneNo IPR availableCDDcd059044CLcoord: 51..544
e-value: 0.0
score: 633.888
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 36..553
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 216..227

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g168780.m01Csor.00g168780.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046949 fatty-acyl-CoA biosynthetic process
biological_process GO:0001676 long-chain fatty acid metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004467 long-chain fatty acid-CoA ligase activity