Csor.00g168730 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g168730
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	alpha-ketoglutarate-dependent dioxygenase alkB
Location	Csor_Chr18: 8419530 .. 8421887 (-)
RNA-Seq Expression	Csor.00g168730
Synteny	Csor.00g168730

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTACGGTTCAGACAAAGGCACCGACGATTCTGAGCGCACCGCTTTCAGAAGAGCAGAGAAGAAATACAAACTGTACTACGACGACACCTATAAATCTTCCAAAAAGTATATTTCTTCCCAATTTGCGCTTTCAGGTTCTATTTCATTCAATTGGCATTGGATTCGTTGTTAGAGGCGATGTTTCATACTCTTGCTTATGATTCCTTTCAGGAAAAAGCTACCGAAACAAGTGGATTTGTCTGAGGTTATCGATTTCAAGCGTATCTTCGAATGTTACAATCAGGATGGTGCGCTTCCGCTGGGCGTGAATGCAACTAAGTGCGATCTCGATGGACCAGTCTTCTGCTTGGAGAATCGTCCTGGTATTGCTAATTCTCCATTTTCGATAGTGTTTCAAAGAAAAAGATCCCAAAATTCAGTTGTTCTCTATGTGTCTAAAATGTAGGGTTTTATTTTATTCCTGGTGCGTTGAGTTTAGAAGAACAATGCCAATGGATTAGGAAGAGTTTAAAGGATTTCCCGCAGCCTCCTAACAGAACCAATCATAATGCTATTTATGGACCAATTCAAGACCTGTTCATTGCTGCTAAGGAAAAGAGAGTTTTAGTTGAAGATAATGAGATCTCTGCTTCCAACGTTGATTCCGACACTGAACCTTCCGTCATCAATGGAAATTCTAATGGATGGAAGTTTGTGGAGGAAAATACTGTTTCATCCAGGAGGGGGACCTGCAAATCAGTTCCGGCCTCTGCATTACTTCGGAAGTTGCGTTGGAGTACACTCGGCCTACAATTTGATTGGTCCAAGGTGAGTTTAACTAGAAGAATCCATATTTTACCCGTGTTTTATGTATGAACTCTTTGTAATATTAGGAGTGTTGAGAATTGTTAGGAGGGAGTCCCACGTTGGGCTAATTTAGGGGATGATTATGAGTTTATAAATAAGGAATACATCTCCATTGGTACGAGGCCTTTTGGGAAAACCAAAAGTAAAGTCATGAGAGCTTATGCTCAAAATGGACAATATCATACCATTTTGGAGGTTCGTGAATCCTAACATGGTATTAGAGTCATGCCCTTAATTTAGCAATGTCAATAGAATCCTCAAGTGTCGAACAAAGAAATTGCTAGTCTCGTAAGTGTAGTAAAAAATGACTCATGTGTCGAACAAAGGGTGTACTTTGTTTGAGGGCTTGAGAGAAAGGAGTCGAGCCTCGATTAAGGAGAGGCTGTCCAAGGGCTCTATAGACCTCAGGGGAGGCTCTATGGTGTACTATGTTCGAGGGGAGGATTGTTGGGAGGGAGTCCCACATTGGCTAATTTAGCGAATGATCATGGGTTTATAAGTAAGGAATACATCTCCATTGGTATGAGGCCTTTTGGGGAATCCCAAATCATAGCCATGAGAGTTCATGCTCCAAGTAGACAATATCATACCATTGTGGAAAGTCGTGATTCCTAACAAGAAGATTATACTGTATCTATGGATTTGTTTGATTTCTGAATTAATCTCCTTGTTATGGAGTAGCGAAGCTATGACATATCTCTGCCCCATAACGAGATTCCCTCTGCGTTATGTCAACTTACCAAAAGAATGGCGGCAGCTGCAATGCCTACTGGGGAAGAATTCAAACCTGAAGCTGCAATAGTGAACTATTTTGCTTCAGGTAAGTAAAGAAAAATTTGTTCATTGACATCAATAGAATGATAAGTAATGCATGGAAATGCCAGCAGAACCTTGCCATTACTCTGACACTTCGTTGATATCTTTAGGCGACACGCTCGGGGGTCACCTAGATGACATGGAAGCTGACTGGAGCAAGCCAATTGTTAGCATGAGGTATCTTGCCAACTTATCCTCATTGAATTGCATTCCTGTCTGTTATGTTAAAGAGGACATTGATCTCCAGTACTTATTCTGTTATCTGCTTTATCCGCCCTCTCCAGTTTGGGTTGCAAGGCTATCTTTCTTTTGGGAGGCAAGTCGAGACAGGATCCGCCCGTAGCCATGTTTCTTCGAAGTGGAGATGTCGTGCTAATGGCTGGAGAAGCAAGGGAATGTTTCCATGGTAATGCAGCCCTCATGTTTACTCTTACATCATGCGTTGCCCTTTTGTTCTATATATCGACCCGGATATCCGATTTCAACGAATGCTCTGTCATTCATCTATCGAAAAAACCGCTAACTTCCTGAAACATGCCGTGAATGTCTTGTAGGTGTACCACGGATCTTCACAGATGAAGAAAGTGAGGAAATTTCTCTTCTTGAAAAGTGGTTTTCAAGTCGAGATGATTTGCATTTTCTGGAATACATAAGAACTTCAAGAATAAACATCAACATTAGACAGGTTTTCTGA

mRNA sequence

ATGTACGGTTCAGACAAAGGCACCGACGATTCTGAGCGCACCGCTTTCAGAAGAGCAGAGAAGAAATACAAACTGTACTACGACGACACCTATAAATCTTCCAAAAAGAAAAAGCTACCGAAACAAGTGGATTTGTCTGAGGTTATCGATTTCAAGCGTATCTTCGAATGTTACAATCAGGATGGTGCGCTTCCGCTGGGCGTGAATGCAACTAAGTGCGATCTCGATGGACCAGTCTTCTGCTTGGAGAATCGTCCTGGGTTTTATTTTATTCCTGGTGCGTTGAGTTTAGAAGAACAATGCCAATGGATTAGGAAGAGTTTAAAGGATTTCCCGCAGCCTCCTAACAGAACCAATCATAATGCTATTTATGGACCAATTCAAGACCTGTTCATTGCTGCTAAGGAAAAGAGAGTTTTAGTTGAAGATAATGAGATCTCTGCTTCCAACGTTGATTCCGACACTGAACCTTCCGTCATCAATGGAAATTCTAATGGATGGAAGTTTGTGGAGGAAAATACTGTTTCATCCAGGAGGGGGACCTGCAAATCAGTTCCGGCCTCTGCATTACTTCGGAAGTTGCGTTGGAGTACACTCGGCCTACAATTTGATTGGTCCAAGCGAAGCTATGACATATCTCTGCCCCATAACGAGATTCCCTCTGCGTTATGTCAACTTACCAAAAGAATGGCGGCAGCTGCAATGCCTACTGGGGAAGAATTCAAACCTGAAGCTGCAATAGTGAACTATTTTGCTTCAGGCGACACGCTCGGGGGTCACCTAGATGACATGGAAGCTGACTGGAGCAAGCCAATTGTTAGCATGAGTTTGGGTTGCAAGGCTATCTTTCTTTTGGGAGGCAAGTCGAGACAGGATCCGCCCGTAGCCATGTTTCTTCGAAGTGGAGATGTCGTGCTAATGGCTGGAGAAGCAAGGGAATGTTTCCATGGTGTACCACGGATCTTCACAGATGAAGAAAGTGAGGAAATTTCTCTTCTTGAAAAGTGGTTTTCAAGTCGAGATGATTTGCATTTTCTGGAATACATAAGAACTTCAAGAATAAACATCAACATTAGACAGGTTTTCTGA

Coding sequence (CDS)

ATGTACGGTTCAGACAAAGGCACCGACGATTCTGAGCGCACCGCTTTCAGAAGAGCAGAGAAGAAATACAAACTGTACTACGACGACACCTATAAATCTTCCAAAAAGAAAAAGCTACCGAAACAAGTGGATTTGTCTGAGGTTATCGATTTCAAGCGTATCTTCGAATGTTACAATCAGGATGGTGCGCTTCCGCTGGGCGTGAATGCAACTAAGTGCGATCTCGATGGACCAGTCTTCTGCTTGGAGAATCGTCCTGGGTTTTATTTTATTCCTGGTGCGTTGAGTTTAGAAGAACAATGCCAATGGATTAGGAAGAGTTTAAAGGATTTCCCGCAGCCTCCTAACAGAACCAATCATAATGCTATTTATGGACCAATTCAAGACCTGTTCATTGCTGCTAAGGAAAAGAGAGTTTTAGTTGAAGATAATGAGATCTCTGCTTCCAACGTTGATTCCGACACTGAACCTTCCGTCATCAATGGAAATTCTAATGGATGGAAGTTTGTGGAGGAAAATACTGTTTCATCCAGGAGGGGGACCTGCAAATCAGTTCCGGCCTCTGCATTACTTCGGAAGTTGCGTTGGAGTACACTCGGCCTACAATTTGATTGGTCCAAGCGAAGCTATGACATATCTCTGCCCCATAACGAGATTCCCTCTGCGTTATGTCAACTTACCAAAAGAATGGCGGCAGCTGCAATGCCTACTGGGGAAGAATTCAAACCTGAAGCTGCAATAGTGAACTATTTTGCTTCAGGCGACACGCTCGGGGGTCACCTAGATGACATGGAAGCTGACTGGAGCAAGCCAATTGTTAGCATGAGTTTGGGTTGCAAGGCTATCTTTCTTTTGGGAGGCAAGTCGAGACAGGATCCGCCCGTAGCCATGTTTCTTCGAAGTGGAGATGTCGTGCTAATGGCTGGAGAAGCAAGGGAATGTTTCCATGGTGTACCACGGATCTTCACAGATGAAGAAAGTGAGGAAATTTCTCTTCTTGAAAAGTGGTTTTCAAGTCGAGATGATTTGCATTTTCTGGAATACATAAGAACTTCAAGAATAAACATCAACATTAGACAGGTTTTCTGA

Protein sequence

MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQDGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNHNAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRGTCKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQVF

Homology

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BLAST of Csor.00g168730 vs. ExPASy Swiss-Prot
Match: Q9SA98 (Alpha-ketoglutarate-dependent dioxygenase alkB OS=Arabidopsis thaliana OX=3702 GN=At1g11780 PE=2 SV=2)

HSP 1 Score: 497.7 bits (1280), Expect = 1.1e-139
Identity = 245/363 (67.49%), Postives = 280/363 (77.13%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60
           MY S   +DD++RTAFRRAEKKYKLYY+   K S+KKKLPK +DLSE++DF  I + +N 
Sbjct: 1   MYESANVSDDADRTAFRRAEKKYKLYYEQDSKFSRKKKLPKPIDLSELLDFNLISQNFNN 60

Query: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120
           DG LP G+  +K D   PVFC++NRPGFYFIP ALSL+EQC+WI++SL  FPQPPNRTNH
Sbjct: 61  DGVLPDGIRVSKVD-SSPVFCIDNRPGFYFIPDALSLKEQCKWIKESLTSFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTV-SSRR 180
           NAIYGPI DLF +AKE +VLV+D+                   +N WKF EE  +  + R
Sbjct: 121 NAIYGPIDDLFDSAKENKVLVQDDL-----------------TNNKWKFYEEVDIEKATR 180

Query: 181 GTCKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGE 240
            +CKSV AS LLRKLRWSTLGLQFDWSKR+YD+SLPHN IP ALCQL K  AA AMP GE
Sbjct: 181 SSCKSVSASVLLRKLRWSTLGLQFDWSKRNYDVSLPHNNIPDALCQLAKTHAAIAMPDGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EF+PE AIVNYF  GDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKS+ DPP AM+L
Sbjct: 241 EFRPEGAIVNYFGIGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSKDDPPHAMYL 300

Query: 301 RSGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIR 360
           RSGDVVLMAGEARECFHG+PRIFT EE+ +I  LE   S      F EYI+TSRININIR
Sbjct: 301 RSGDVVLMAGEARECFHGIPRIFTGEENADIGALESELSHESGHFFAEYIKTSRININIR 345

Query: 361 QVF 363
           QVF
Sbjct: 361 QVF 345

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BLAST of Csor.00g168730 vs. ExPASy Swiss-Prot
Match: Q54N08 (Alpha-ketoglutarate-dependent dioxygenase alkB OS=Dictyostelium discoideum OX=44689 GN=alkB PE=2 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 1.8e-44
Identity = 121/377 (32.10%), Postives = 195/377 (51.72%), Query Frame = 0


Query: 14  TAFRRAEKKYKLYYDDTYKSSKKKKLPKQ----VDLSEVIDF-----------KRIFEC- 73
           T F R ++ ++       KS+  K +PK+    +D S V+DF           K I +C 
Sbjct: 36  TEFMRVQRLFR----QVTKSANGKNIPKEKREPIDYSPVLDFHNLENNTEENKKLIIDCT 95

Query: 74  ---------YNQDGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSL 133
                    +N+D    L     K      V+ L+  PGFYFI    +  +Q +WI+ +L
Sbjct: 96  SNVTTHDFEFNRDTEFYLHPREWK------VYGLQGYPGFYFIKSPFTASQQKKWIKHAL 155

Query: 134 KDFPQPPNRTNHNAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWK 193
           +D+  PPN  N    +GPI++L+    EK ++ E+ +    + D + E      + NG  
Sbjct: 156 EDYADPPNNNNITLFHGPIKNLW-KNGEKELINEELKSQGKHDDDEIEQPTRPLDKNG-- 215

Query: 194 FVEENTVSSRRGTCKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTK 253
              E   + R+          LL KL WSTLG Q+ W+ R Y     + E P  L +L +
Sbjct: 216 ---EPLPTYRQ----------LLDKLAWSTLGYQYQWTPRLYSEEF-YEEFPDDLQELVQ 275

Query: 254 RMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGK 313
           ++A A     + +  EAA VN+++    +GGHLDD E +  KPI+S+S G  A+FL+G +
Sbjct: 276 KIAIAT--KFDPYVAEAATVNFYSEDSIMGGHLDDAEQEMEKPIISISFGSTAVFLMGAE 335

Query: 314 SRQDPPVAMFLRSGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHF-LE 363
           +R   PV +F+RSGD+V+M G +R C+HGV +I   E S ++ L+++  +   DL + ++
Sbjct: 336 TRDIAPVPLFIRSGDIVIMGGRSRYCYHGVAKIV--ENSFDLGLIDE--NDDQDLKYKIQ 379

[Close]

BLAST of Csor.00g168730 vs. ExPASy Swiss-Prot
Match: Q13686 (Nucleic acid dioxygenase ALKBH1 OS=Homo sapiens OX=9606 GN=ALKBH1 PE=1 SV=2)

HSP 1 Score: 143.3 bits (360), Expect = 5.5e-33
Identity = 94/295 (31.86%), Postives = 143/295 (48.47%), Query Frame = 0


Query: 82  LENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNHNAIYGPIQDLFIAAKEKRVLV 141
           L+  PGF FIP       Q  W+++ LK + Q PN  N         D  ++ +E + L 
Sbjct: 93  LKGYPGFIFIPNPFLPGYQWHWVKQCLKLYSQKPNVCN--------LDKHMSKEETQDLW 152

Query: 142 EDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRGTCKSVPASALLRKLRWSTLGL 201
           E ++                      +F+     + RR         +LL KLRW T+G 
Sbjct: 153 EQSK----------------------EFLRYKEATKRR-------PRSLLEKLRWVTVGY 212

Query: 202 QFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHL 261
            ++W  + Y     +   PS L  L++++AAA     E+F+ EA I+NY+    TLG H+
Sbjct: 213 HYNWDSKKYSAD-HYTPFPSDLGFLSEQVAAAC--GFEDFRAEAGILNYYRLDSTLGIHV 272

Query: 262 DDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGEARECFHGVPRI 321
           D  E D SKP++S S G  AIFLLGG  R + P AMF+ SGD+++M+G +R   H VPR+
Sbjct: 273 DRSELDHSKPLLSFSFGQSAIFLLGGLQRDEAPTAMFMHSGDIMIMSGFSRLLNHAVPRV 332

Query: 322 FTDEESEEI---------------SLLEKWFSSRDDLHFLEYIRTSRININIRQV 362
             + E E +               S++E   S  D      Y++T+R+N+ +RQV
Sbjct: 333 LPNPEGEGLPHCLEAPLPAVLPRDSMVEP-CSMEDWQVCASYLKTARVNMTVRQV 346

[Close]

BLAST of Csor.00g168730 vs. ExPASy Swiss-Prot
Match: P0CB42 (Nucleic acid dioxygenase ALKBH1 OS=Mus musculus OX=10090 GN=Alkbh1 PE=1 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 3.5e-32
Identity = 108/357 (30.25%), Postives = 160/357 (44.82%), Query Frame = 0


Query: 36  KKKLPKQVDLSEVIDFKRIFECYNQDGALPLGV-------NATKCDLD----GPV----- 95
           ++  P   DL  VIDF       +    +P  V       + T+ D +     PV     
Sbjct: 31  RQSRPGTADLGAVIDFSEAHLARSPKPGVPQVVRFPLNVSSVTERDAERVGLEPVSKWRA 90

Query: 96  FCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNHNAIYGPIQDLFIAAKEKRV 155
           + LE  PGF FIP       Q  W+++ LK + Q PN  N         D  +  +E + 
Sbjct: 91  YGLEGYPGFIFIPNPFLPGCQRHWVKQCLKLYSQKPNVCN--------LDKHMTKEETQG 150

Query: 156 LVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRGTCKSVPASALLRKLRWSTL 215
           L E ++                      + +    V+ RR         +LL +LRW TL
Sbjct: 151 LWEQSK----------------------EVLRSKEVTKRR-------PRSLLERLRWVTL 210

Query: 216 GLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVNYFASGDTLGG 275
           G  ++W  + Y     +   PS L  L++++A A    G  F+ EA I+NY+    TLG 
Sbjct: 211 GYHYNWDSKKYSAD-HYTPFPSDLAFLSEQVATACGFQG--FQAEAGILNYYRLDSTLGI 270

Query: 276 HLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGEARECFHGVP 335
           H+D  E D SKP++S S G  AIFLLGG  R + P AMF+ SGD+++M+G +R   H VP
Sbjct: 271 HVDRSELDHSKPLLSFSFGQSAIFLLGGLKRDEAPTAMFMHSGDIMVMSGFSRLLNHAVP 330

Query: 336 RIFTDEESEEI---------------SLLEKWFSSRDDLHFLEYIRTSRININIRQV 362
           R+    + E +               SL+E   S  D      Y+RT+R+N+ +RQV
Sbjct: 331 RVLPHPDGECLPHCLETPLPAVLPSNSLVEP-CSVEDWQVCATYLRTARVNMTVRQV 346

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BLAST of Csor.00g168730 vs. ExPASy Swiss-Prot
Match: O60066 (Alpha-ketoglutarate-dependent dioxygenase abh1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=abh1 PE=2 SV=3)

HSP 1 Score: 130.2 bits (326), Expect = 4.8e-29
Identity = 98/352 (27.84%), Postives = 158/352 (44.89%), Query Frame = 0


Query: 12  ERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQDGALPLGVNAT 71
           +   FR  EK+YK   D          +P   D+SEV+D          +     G  A 
Sbjct: 8   QANVFRLEEKRYKCRAD---------TIP---DMSEVLD---------PNDPQSFGFEAL 67

Query: 72  KCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSL-KDFPQPPNRTNHNAIYGPIQDL 131
             ++   VF  +  PG   +   +S E Q Q ++  +      P N+TN +  Y      
Sbjct: 68  -VEIKPRVFSFQKAPGLLILKNYVSSELQMQLLKSIMFTQIQDPENKTNLSPFY------ 127

Query: 132 FIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRGTCKSVPASAL 191
                  ++ + ++ I     + D E S+I+G                 G  K +    L
Sbjct: 128 -------QLPLGNDSIWRRYYNGDGE-SIIDG----------------LGETKPLTVDRL 187

Query: 192 L-RKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVN 251
           + +KLRW TLG Q+DW+ + Y         P  L    +++   +      +K EAAIVN
Sbjct: 188 VHKKLRWVTLGEQYDWTTKEYPDPSKSPGFPKDLGDFVEKVVKESTDF-LHWKAEAAIVN 247

Query: 252 YFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAG 311
           +++ GDTL  H+D+ E D + P++S+S+G   I+L+G +SR + P A+ L SGDVV+M G
Sbjct: 248 FYSPGDTLSAHIDESEEDLTLPLISLSMGLDCIYLIGTESRSEKPSALRLHSGDVVIMTG 298

Query: 312 EARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQV 362
            +R+ FH VP+I  +     +    K +          +I   R+N N+RQV
Sbjct: 308 TSRKAFHAVPKIIPNSTPNYLLTGNKAWDG--------WISRKRVNFNVRQV 298

[Close]

BLAST of Csor.00g168730 vs. NCBI nr
Match: KAG6573589.1 (Alpha-ketoglutarate-dependent dioxygenase alkB, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 739 bits (1909), Expect = 1.78e-269
Identity = 362/362 (100.00%), Postives = 362/362 (100.00%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60
           MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60

Query: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120
           DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH
Sbjct: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180
           NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180

Query: 181 TCKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGEE 240
           TCKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGEE
Sbjct: 181 TCKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGEE 240

Query: 241 FKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR 300
           FKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR
Sbjct: 241 FKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR 300

Query: 301 SGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQ 360
           SGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQ
Sbjct: 301 SGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQ 360

Query: 361 VF 362
           VF
Sbjct: 361 VF 362

[Close]

BLAST of Csor.00g168730 vs. NCBI nr
Match: XP_022945036.1 (alpha-ketoglutarate-dependent dioxygenase alkB [Cucurbita moschata])

HSP 1 Score: 728 bits (1879), Expect = 6.65e-265
Identity = 357/362 (98.62%), Postives = 360/362 (99.45%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60
           MYGSDKGTDD+ERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRI ECYNQ
Sbjct: 1   MYGSDKGTDDAERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRILECYNQ 60

Query: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120
           DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIR+SLKDFPQPPNRTNH
Sbjct: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLKDFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180
           NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPS+INGNSNGWKFVEENTVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSLINGNSNGWKFVEENTVSSRRG 180

Query: 181 TCKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGEE 240
           TCKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGEE
Sbjct: 181 TCKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGEE 240

Query: 241 FKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR 300
           FKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR
Sbjct: 241 FKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR 300

Query: 301 SGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQ 360
           SGDVVLMAGEARECFHGVPRIFTDEESEEISLLEK FSSRDDLHFLEYIRTSRININIRQ
Sbjct: 301 SGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKRFSSRDDLHFLEYIRTSRININIRQ 360

Query: 361 VF 362
           VF
Sbjct: 361 VF 362

[Close]

BLAST of Csor.00g168730 vs. NCBI nr
Match: XP_023541203.1 (alpha-ketoglutarate-dependent dioxygenase alkB [Cucurbita pepo subsp. pepo])

HSP 1 Score: 720 bits (1858), Expect = 1.06e-261
Identity = 354/362 (97.79%), Postives = 358/362 (98.90%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60
           MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRI ECYNQ
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRILECYNQ 60

Query: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120
           DGALPLGVNATKCDLDGPVFCLENRPGFYFI GALSLEEQCQWIR+SLKDFPQPPNRTNH
Sbjct: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFISGALSLEEQCQWIRESLKDFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180
           NAIYGPIQDLFIAAKEK+VLVEDNEISASNVDSDTEPS+INGNSNGWKFVEENTVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKEKKVLVEDNEISASNVDSDTEPSLINGNSNGWKFVEENTVSSRRG 180

Query: 181 TCKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGEE 240
           TCKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGEE
Sbjct: 181 TCKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGEE 240

Query: 241 FKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR 300
           FKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVS+SLGCKAIFLLGGKSRQD PVAMFLR
Sbjct: 241 FKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSLSLGCKAIFLLGGKSRQDLPVAMFLR 300

Query: 301 SGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQ 360
           SGDVVLMAGEARECFHGVPRIFTDEESEEISLLEK FSSRDDLHFLEYIRTSRININIRQ
Sbjct: 301 SGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKRFSSRDDLHFLEYIRTSRININIRQ 360

Query: 361 VF 362
           VF
Sbjct: 361 VF 362

[Close]

BLAST of Csor.00g168730 vs. NCBI nr
Match: XP_022966806.1 (alpha-ketoglutarate-dependent dioxygenase alkB [Cucurbita maxima])

HSP 1 Score: 717 bits (1850), Expect = 1.75e-260
Identity = 354/362 (97.79%), Postives = 357/362 (98.62%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60
           MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRI ECYNQ
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRILECYNQ 60

Query: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120
           DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQ IR+SLKDFPQPPNRTNH
Sbjct: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQRIRESLKDFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180
           NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180

Query: 181 TCKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGEE 240
           TCKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHN+IPSALCQL KRMAAAAMPTGEE
Sbjct: 181 TCKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNDIPSALCQLAKRMAAAAMPTGEE 240

Query: 241 FKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR 300
           FKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQD PVAMFLR
Sbjct: 241 FKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDLPVAMFLR 300

Query: 301 SGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQ 360
           SGDVVLMAGEARECFHGVPRIFTDEESEEISLLEK FSSRDDLHFLEY+RTSRININIRQ
Sbjct: 301 SGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKRFSSRDDLHFLEYMRTSRININIRQ 360

Query: 361 VF 362
           VF
Sbjct: 361 VF 362

[Close]

BLAST of Csor.00g168730 vs. NCBI nr
Match: KAG7012728.1 (Alpha-ketoglutarate-dependent dioxygenase alkB [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 716 bits (1848), Expect = 2.31e-259
Identity = 362/412 (87.86%), Postives = 362/412 (87.86%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60
           MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60

Query: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120
           DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH
Sbjct: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180
           NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180

Query: 181 TCKSVPASALLRKLRWSTLGLQFDWSK--------------------------------- 240
           TCKSVPASALLRKLRWSTLGLQFDWSK                                 
Sbjct: 181 TCKSVPASALLRKLRWSTLGLQFDWSKEGVPRWANLGDDYEFINKEYISIGTRPFGKTKS 240

Query: 241 -----------------RSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVNY 300
                            RSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVNY
Sbjct: 241 KVMRAYAQSGQYHTILERSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVNY 300

Query: 301 FASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGE 360
           FASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGE
Sbjct: 301 FASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGE 360

Query: 361 ARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQVF 362
           ARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQVF
Sbjct: 361 ARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQVF 412

[Close]

BLAST of Csor.00g168730 vs. ExPASy TrEMBL
Match: A0A6J1FZQ9 (alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucurbita moschata OX=3662 GN=LOC111449397 PE=4 SV=1)

HSP 1 Score: 728 bits (1879), Expect = 3.22e-265
Identity = 357/362 (98.62%), Postives = 360/362 (99.45%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60
           MYGSDKGTDD+ERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRI ECYNQ
Sbjct: 1   MYGSDKGTDDAERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRILECYNQ 60

Query: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120
           DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIR+SLKDFPQPPNRTNH
Sbjct: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLKDFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180
           NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPS+INGNSNGWKFVEENTVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSLINGNSNGWKFVEENTVSSRRG 180

Query: 181 TCKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGEE 240
           TCKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGEE
Sbjct: 181 TCKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGEE 240

Query: 241 FKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR 300
           FKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR
Sbjct: 241 FKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR 300

Query: 301 SGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQ 360
           SGDVVLMAGEARECFHGVPRIFTDEESEEISLLEK FSSRDDLHFLEYIRTSRININIRQ
Sbjct: 301 SGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKRFSSRDDLHFLEYIRTSRININIRQ 360

Query: 361 VF 362
           VF
Sbjct: 361 VF 362

[Close]

BLAST of Csor.00g168730 vs. ExPASy TrEMBL
Match: A0A6J1HQB4 (alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucurbita maxima OX=3661 GN=LOC111466401 PE=4 SV=1)

HSP 1 Score: 717 bits (1850), Expect = 8.49e-261
Identity = 354/362 (97.79%), Postives = 357/362 (98.62%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60
           MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRI ECYNQ
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRILECYNQ 60

Query: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120
           DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQ IR+SLKDFPQPPNRTNH
Sbjct: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQRIRESLKDFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180
           NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180

Query: 181 TCKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGEE 240
           TCKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHN+IPSALCQL KRMAAAAMPTGEE
Sbjct: 181 TCKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNDIPSALCQLAKRMAAAAMPTGEE 240

Query: 241 FKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR 300
           FKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQD PVAMFLR
Sbjct: 241 FKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDLPVAMFLR 300

Query: 301 SGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQ 360
           SGDVVLMAGEARECFHGVPRIFTDEESEEISLLEK FSSRDDLHFLEY+RTSRININIRQ
Sbjct: 301 SGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKRFSSRDDLHFLEYMRTSRININIRQ 360

Query: 361 VF 362
           VF
Sbjct: 361 VF 362

[Close]

BLAST of Csor.00g168730 vs. ExPASy TrEMBL
Match: A0A6J1DG90 (alpha-ketoglutarate-dependent dioxygenase alkB OS=Momordica charantia OX=3673 GN=LOC111019811 PE=4 SV=1)

HSP 1 Score: 669 bits (1726), Expect = 6.73e-242
Identity = 328/362 (90.61%), Postives = 341/362 (94.20%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60
           MYGSDKGTDD ERTAFR AEKKYK YYDDT+KSSKKKKLPKQVDLSEVIDFKRI ECYN 
Sbjct: 1   MYGSDKGTDDLERTAFRIAEKKYKFYYDDTFKSSKKKKLPKQVDLSEVIDFKRILECYNH 60

Query: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120
           DGALPLG+NAT+CDLDGPVFCLENRPGFYFIPGALSLEEQCQWIR+SL  FPQPPNRTNH
Sbjct: 61  DGALPLGMNATQCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLTSFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180
           NAIYGPIQDLFIAAKEKRVLVE NEISA NVDSD EPSV  GN++GWKFVEENTVSSRR 
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEANEISAFNVDSDIEPSVSTGNTHGWKFVEENTVSSRRT 180

Query: 181 TCKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGEE 240
           TCKSVPAS LLRKLRWSTLGLQFDWSKRSYDISL HN++PSALCQL KRMAAAAMP GEE
Sbjct: 181 TCKSVPASVLLRKLRWSTLGLQFDWSKRSYDISLLHNKMPSALCQLAKRMAAAAMPAGEE 240

Query: 241 FKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR 300
           FKPEAAIVNYFASGD+LGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR
Sbjct: 241 FKPEAAIVNYFASGDSLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR 300

Query: 301 SGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQ 360
           SGDVVLMAGEARECFHGVPRIF D E++E SLLEK FS++DDLHFLEYIRTSRININIRQ
Sbjct: 301 SGDVVLMAGEARECFHGVPRIFIDVETDETSLLEKQFSNQDDLHFLEYIRTSRININIRQ 360

Query: 361 VF 362
           VF
Sbjct: 361 VF 362

[Close]

BLAST of Csor.00g168730 vs. ExPASy TrEMBL
Match: A0A1S3BE32 (LOW QUALITY PROTEIN: alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucumis melo OX=3656 GN=LOC103488884 PE=4 SV=1)

HSP 1 Score: 656 bits (1693), Expect = 7.46e-237
Identity = 321/363 (88.43%), Postives = 339/363 (93.39%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60
           MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPK VDLSEVIDFK I E Y Q
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYKQ 60

Query: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120
           DG+LP+GV AT CDLD PVFCLENRPGFYFIPGALSL+EQCQWIR+SL DFPQPPNRTNH
Sbjct: 61  DGSLPVGVKATTCDLDRPVFCLENRPGFYFIPGALSLQEQCQWIRESLMDFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180
           NAIYGPIQDLFIAAKEK+VLVE +EIS  N+DSD EPS+ NG+++ WKFVEENTVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKEKKVLVEHDEISDFNLDSDVEPSISNGSTHNWKFVEENTVSSRRG 180

Query: 181 T-CKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGE 240
           T CKS+ AS LLRKLRWSTLGLQFDWSKRSY+ISLPHN+IPSALCQL KRMAAAAMPTGE
Sbjct: 181 TACKSISASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLAKRMAAAAMPTGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKA FLLGGKSRQDPP+AMFL
Sbjct: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAXFLLGGKSRQDPPIAMFL 300

Query: 301 RSGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIR 360
           RSGDVVLMAGEARECFHGVPRIF DEESEEIS LE+  S++DDLHFLEYIRTSRININIR
Sbjct: 301 RSGDVVLMAGEARECFHGVPRIFIDEESEEISFLERHLSNQDDLHFLEYIRTSRININIR 360

Query: 361 QVF 362
           QVF
Sbjct: 361 QVF 363

[Close]

BLAST of Csor.00g168730 vs. ExPASy TrEMBL
Match: A0A0A0KS00 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G589370 PE=4 SV=1)

HSP 1 Score: 654 bits (1687), Expect = 6.13e-236
Identity = 320/363 (88.15%), Postives = 338/363 (93.11%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60
           MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPK VDLSEVIDFK I E Y Q
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYQQ 60

Query: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120
           DG+LP+GVNAT CDLDGPVFCLENRPGFYFIPGALSL+EQCQWIR+SL +FPQPPNRTNH
Sbjct: 61  DGSLPVGVNATTCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLMEFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180
           NAIYGPIQDLFIAAKE +VLVE +EIS   +DSD EPS+ NGN++ WKFVEENTVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWKFVEENTVSSRRG 180

Query: 181 TC-KSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGE 240
           T  KS+PAS LLRKLRWSTLGLQFDWSKRSY+ISLPHN+IPSALCQL KRMAAAAMPTGE
Sbjct: 181 TAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLAKRMAAAAMPTGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPP+AMFL
Sbjct: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPIAMFL 300

Query: 301 RSGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIR 360
           RSGDVVLMAGEARECFHGVPRIF DEESEEIS LE   +++DDLH LEYIRTSRININIR
Sbjct: 301 RSGDVVLMAGEARECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLEYIRTSRININIR 360

Query: 361 QVF 362
           QVF
Sbjct: 361 QVF 363

[Close]

BLAST of Csor.00g168730 vs. TAIR 10
Match: AT1G11780.1 (oxidoreductase, 2OG-Fe(II) oxygenase family protein )

HSP 1 Score: 497.7 bits (1280), Expect = 8.1e-141
Identity = 245/363 (67.49%), Postives = 280/363 (77.13%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60
           MY S   +DD++RTAFRRAEKKYKLYY+   K S+KKKLPK +DLSE++DF  I + +N 
Sbjct: 1   MYESANVSDDADRTAFRRAEKKYKLYYEQDSKFSRKKKLPKPIDLSELLDFNLISQNFNN 60

Query: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120
           DG LP G+  +K D   PVFC++NRPGFYFIP ALSL+EQC+WI++SL  FPQPPNRTNH
Sbjct: 61  DGVLPDGIRVSKVD-SSPVFCIDNRPGFYFIPDALSLKEQCKWIKESLTSFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTV-SSRR 180
           NAIYGPI DLF +AKE +VLV+D+                   +N WKF EE  +  + R
Sbjct: 121 NAIYGPIDDLFDSAKENKVLVQDDL-----------------TNNKWKFYEEVDIEKATR 180

Query: 181 GTCKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGE 240
            +CKSV AS LLRKLRWSTLGLQFDWSKR+YD+SLPHN IP ALCQL K  AA AMP GE
Sbjct: 181 SSCKSVSASVLLRKLRWSTLGLQFDWSKRNYDVSLPHNNIPDALCQLAKTHAAIAMPDGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EF+PE AIVNYF  GDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKS+ DPP AM+L
Sbjct: 241 EFRPEGAIVNYFGIGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSKDDPPHAMYL 300

Query: 301 RSGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIR 360
           RSGDVVLMAGEARECFHG+PRIFT EE+ +I  LE   S      F EYI+TSRININIR
Sbjct: 301 RSGDVVLMAGEARECFHGIPRIFTGEENADIGALESELSHESGHFFAEYIKTSRININIR 345

Query: 361 QVF 363
           QVF
Sbjct: 361 QVF 345

[Close]

BLAST of Csor.00g168730 vs. TAIR 10
Match: AT3G14160.1 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 66.6 bits (161), Expect = 4.6e-11
Identity = 42/122 (34.43%), Postives = 63/122 (51.64%), Query Frame = 0


Query: 243 PEAAIVNYFASGDTLGGHLDDMEADWS----KPIVSMSLGCKAIFLLGGKSRQDPPVAMF 302
           P+  IVN+++S   LG H D  E++ S     P+VS S+G  A FL G +  +D    + 
Sbjct: 346 PDICIVNFYSSTGRLGLHQDKDESENSIRKGLPVVSFSIGDSAEFLYGDQRDEDKAETLT 405

Query: 303 LRSGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININI 361
           L SGDV+L  G +R+ FHGV  I  D   +  +LL++             +R  R+N+  
Sbjct: 406 LESGDVLLFGGRSRKVFHGVRSIRKDTAPK--ALLQE-----------TSLRPGRLNLTF 454

[Close]

BLAST of Csor.00g168730 vs. TAIR 10
Match: AT5G01780.1 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 66.6 bits (161), Expect = 4.6e-11
Identity = 43/121 (35.54%), Postives = 61/121 (50.41%), Query Frame = 0


Query: 243 PEAAIVNYFASGDTLGGHLDDMEADWS----KPIVSMSLGCKAIFLLGGKSRQDPPVAMF 302
           P+  IVN+++    LG H D  E++ S     PIVS S+G  A FL G K   +    + 
Sbjct: 278 PDICIVNFYSETGRLGLHQDRDESEESIARGLPIVSFSIGDSAEFLYGEKRDVEEAQGVI 337

Query: 303 LRSGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININI 360
           L SGDV++  GE+R  FHGV  I  +  S  +SLL +             +RT R+N+  
Sbjct: 338 LESGDVLIFGGESRMIFHGVKSIIPN--SAPMSLLNE-----------SKLRTGRLNLTF 385

[Close]

BLAST of Csor.00g168730 vs. TAIR 10
Match: AT5G01780.2 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 66.6 bits (161), Expect = 4.6e-11
Identity = 43/121 (35.54%), Postives = 61/121 (50.41%), Query Frame = 0


Query: 243 PEAAIVNYFASGDTLGGHLDDMEADWS----KPIVSMSLGCKAIFLLGGKSRQDPPVAMF 302
           P+  IVN+++    LG H D  E++ S     PIVS S+G  A FL G K   +    + 
Sbjct: 333 PDICIVNFYSETGRLGLHQDRDESEESIARGLPIVSFSIGDSAEFLYGEKRDVEEAQGVI 392

Query: 303 LRSGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININI 360
           L SGDV++  GE+R  FHGV  I  +  S  +SLL +             +RT R+N+  
Sbjct: 393 LESGDVLIFGGESRMIFHGVKSIIPN--SAPMSLLNE-----------SKLRTGRLNLTF 440

[Close]

BLAST of Csor.00g168730 vs. TAIR 10
Match: AT3G14140.1 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 48.9 bits (115), Expect = 9.9e-06
Identity = 32/104 (30.77%), Postives = 43/104 (41.35%), Query Frame = 0


Query: 243 PEAAIVNYFASGDTLGGH----LDDMEADWSK---------------------PIVSMSL 302
           P+  +VN++ S   LG H     D    D+ K                     PIVS S+
Sbjct: 351 PDICVVNFYTSTGKLGLHQVSVYDKTSFDFLKYKGGYLNTDKGESKKSLRKGLPIVSFSI 410

Query: 303 GCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGEARECFHGVPRI 322
           G  A FL G +   D    + L SGDV++    +R  FHGV  I
Sbjct: 411 GDSAEFLYGDQKDVDKADTLILESGDVLIFGERSRNVFHGVRSI 454

The following BLAST results are available for this feature:

BLAST of Csor.00g168730 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SA98	1.1e-139	67.49	Alpha-ketoglutarate-dependent dioxygenase alkB OS=Arabidopsis thaliana OX=3702 G...	[more]
Q54N08	1.8e-44	32.10	Alpha-ketoglutarate-dependent dioxygenase alkB OS=Dictyostelium discoideum OX=44...	[more]
Q13686	5.5e-33	31.86	Nucleic acid dioxygenase ALKBH1 OS=Homo sapiens OX=9606 GN=ALKBH1 PE=1 SV=2	[more]
P0CB42	3.5e-32	30.25	Nucleic acid dioxygenase ALKBH1 OS=Mus musculus OX=10090 GN=Alkbh1 PE=1 SV=1	[more]
O60066	4.8e-29	27.84	Alpha-ketoglutarate-dependent dioxygenase abh1 OS=Schizosaccharomyces pombe (str...	[more]

BLAST of Csor.00g168730 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6573589.1	1.78e-269	100.00	Alpha-ketoglutarate-dependent dioxygenase alkB, partial [Cucurbita argyrosperma ...	[more]
XP_022945036.1	6.65e-265	98.62	alpha-ketoglutarate-dependent dioxygenase alkB [Cucurbita moschata]	[more]
XP_023541203.1	1.06e-261	97.79	alpha-ketoglutarate-dependent dioxygenase alkB [Cucurbita pepo subsp. pepo]	[more]
XP_022966806.1	1.75e-260	97.79	alpha-ketoglutarate-dependent dioxygenase alkB [Cucurbita maxima]	[more]
KAG7012728.1	2.31e-259	87.86	Alpha-ketoglutarate-dependent dioxygenase alkB [Cucurbita argyrosperma subsp. ar...	[more]

BLAST of Csor.00g168730 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FZQ9	3.22e-265	98.62	alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1HQB4	8.49e-261	97.79	alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1DG90	6.73e-242	90.61	alpha-ketoglutarate-dependent dioxygenase alkB OS=Momordica charantia OX=3673 GN...	[more]
A0A1S3BE32	7.46e-237	88.43	LOW QUALITY PROTEIN: alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucumis m...	[more]
A0A0A0KS00	6.13e-236	88.15	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G...	[more]

BLAST of Csor.00g168730 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G11780.1	8.1e-141	67.49	oxidoreductase, 2OG-Fe(II) oxygenase family protein	[more]
AT3G14160.1	4.6e-11	34.43	2-oxoglutarate-dependent dioxygenase family protein	[more]
AT5G01780.1	4.6e-11	35.54	2-oxoglutarate-dependent dioxygenase family protein	[more]
AT5G01780.2	4.6e-11	35.54	2-oxoglutarate-dependent dioxygenase family protein	[more]
AT3G14140.1	9.9e-06	30.77	2-oxoglutarate-dependent dioxygenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027450	Alpha-ketoglutarate-dependent dioxygenase AlkB-like	PFAM	PF13532	2OG-FeII_Oxy_2	coord: 186..359 e-value: 1.1E-40 score: 140.0
IPR037151	Alpha-ketoglutarate-dependent dioxygenase AlkB-like superfamily	GENE3D	2.60.120.590		coord: 163..361 e-value: 1.8E-48 score: 166.9
IPR004574	Alkylated DNA repair protein AlkB	PANTHER	PTHR16557	ALKYLATED DNA REPAIR PROTEIN ALKB-RELATED	coord: 1..362
None	No IPR available	PANTHER	PTHR16557:SF8	ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB	coord: 1..362
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 86..361
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 242..362 score: 9.285449

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g168730.m01	Csor.00g168730.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006281	DNA repair
biological_process	GO:0035513	oxidative RNA demethylation
biological_process	GO:0035552	oxidative single-stranded DNA demethylation
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0008198	ferrous iron binding
molecular_function	GO:0035516	oxidative DNA demethylase activity
molecular_function	GO:0035515	oxidative RNA demethylase activity