Csor.00g166880 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g166880
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionFerrochelatase
LocationCsor_Chr05: 454496 .. 457519 (-)
RNA-Seq ExpressionCsor.00g166880
SyntenyCsor.00g166880
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGCGACCTCTTCCTCTGGCGCTCTTTCTAATATGAAGCTTCTCAGTTCCAATAAACTCAATTCGGATCACAGGGTTCCCACGTAAGTTGTATAATGTATCACTTGTGCCTTCCTTCAGCCTTTGCTTGTAGTTTTCGAATGATGATCGGATTCTTAAGTCGCCTTTTATTGTTACTTAGGAAATTTTCGTTTTATATTATCTGTAGCGTCTGGAAATTTCCGCTCCATATGAACTTTATGTTGATTATTGGATGAAATTACCATATGTTGCAATCTTCTTGCAGCCTGAAACTAGGCATATGAATTTAATTCTGCAAATTTGGAACCCGTCCCCTTAGCTCGATCACTGTGAAGTTGTGTGAATGATAATAACAGGGTTGACCAGAATGAACGTTCTTTGAATTCAAGATGCCACGGCAATGGCATATCTTTTTATAGAAGTTTTCAGGTATTTTATTATCAAAGTTAAGAATTCAACATTTGTTTTTCCTTTTTCAGGTTATTCTCCTTGCCGAAACCTCTCGTGTCTTTATCTTGCAAATCCTATAGAAATTTGCAAGTTCGTGATAAGTCTACATCTCAAGGATTAGTGGTTTCTTGTTCTAGCTCTAATAGTCACAGAGATGTGATGCAGGGGTTTCACCTTTCTGGCCCCATTGAGAAGAGAAGCCGTCTTGGACAAGCATTTTGCTCGGTAGAAAGTTTCACCTATGGTGAATATGCTTTAGAATCTCGCTCTCAGGCAGCGGAAGAGAAGGTTGGAGTTCTGCTTCTGAATCTAGGAGGGCCAGACACGCTTGATGATGTTCAGCCATTTCTGTACAATCTATTTGCAGATCCGGTATCTTAGTTAATATTATGTATCTAGAGCCTTAATAACATACTAGCGTGTCAACATATATCAGTTCTCACTTATTTGCATTAAACATAGGATATTATCCGGCTCCCAAGGTTGTTCCGTTTCCTCCAAGAGCCATTGGCCAAATTAATATCTACCTTTCGTGCTCCCAAAAGTAAAGAAGGGTATGCTTCAATAGGAGGCGGCTCACCTTTGCGTAAAATAACAGATGAGCAGGTTGATTGACCTCTCTCTTGTTCCCTTCTACTTGTTCATTATGTCATATCTCCAGTTGGGTATAAGAAAATCTTCAAATATTTATAATTTGTGTATACTGGATGCAGGCACAGGCGCTTAAAATGGCTTTGGAAGAGAAAAACACACCTGCTAATGTCTATGTGGGAATGCGCTATTGGTACCCTTTCACAGAGGAAGCAATTCAGCAAGTACGTTCTACATTTTTTTCTTATTTCCCTTGCCTTCTAATTCAAATGCAAGGGGAAATTTTATATTTACTACAGACCACGTGTTATTTCTCAATGTGCCTGTATCTTCAGACTTTAGCATCGTATTTTAGAGATGTTTGAAGTCCTTAATAGTCACTATATTTATTTTAAATATTGGTGGTACCTTTTGCTGTTTGTGGCAGATTAAGAGGGATGGGATAACAAGACTTGTAGTGCTGCCATTATATCCACAGTACTCTATTTCCACAACTGGGTCTAGTATTCGTGTTCTCCAGAAGATTTTCAGGTGATGACTCAGTCCAGTATTACAATATTTTGATTTTATTTTTTTAAAAGAAAAGAGGGATAAAATTGCTTCCAACTTATTGACTATGACTGTCTATCAGGGAAGATGCATATCTCTCAAAATTGCCTGTTTCAGTTATAAAGTCCTGGTATCAACGTGAAGGGTATATTAAGTCCATGGCTGACTTGGTTCAGGCAGAGCTGAAGAATTTTATCAATCCTCAAGAGGTCCTTGATCTTTGCTGAAACTGATTTTAGAGAGATACTTTAGTTTATGCAGCATGATTGTCCCAGTCAATTATTTTCTTATATATAGCATCATTTGCACCGTAGGTCATGATTTTCTTTAGTGCACATGGGGTTCCCGTCAGTTATGTTGAGAATGCTGGAGATCCATACAAAGATCAAATGGAGGAGTGCATCTACCTAATCATGCAAGAGCTGAAAGCCAGAGGAATTGGAAATGAGCATACTCTTGCTTATCAGGTTAATAATAAATTTTTAGCTTCGCTTGGTTTCTTATCCTTTTCTTGCTTTCAACTATTCAGATGTTCTTTATTTCTTTGTTCCTAAGTACACGCATGTGGAATTTCATTCTTGGTTATGTATTTCATGTTCAGAGCCGAGTTGGACCTGTACAATGGCTGAAGCCATACACAGATGAAGTTCTTGTTGAGCTTGGCCAGAAGGGTATTAAGAGTCTCCTAGCCGTTCCTGTGAGGTATGGTTTGTTCATAAACTATAAATGGATGAACTGTTATCGATTGTTGATTTTGATTCCATGTATGTTCATAATTCTAGGAGGTCCTATTATTGCGTATAATTTAAACTATTCATAATCTTGGTAATCATATCTGACCTTAGAGTGAATGTCAGTTGGACTAAATCATATAAAAAGTATATATATTCGTGCGAGCCTAACAATGCAGAAGCTTTGGCCGAATAAGCATTATGCTGTTTGAACGTATTTACAATTGGTTTTACTTGTTTCGAAGACGTGGAGAAGGCAAATGTTCACAGCAAAGTTCGATTTTTCTCGCATTCCTACATTGTTTTCTTTATTCTTCTGCCTTTCTGGCGTTTTATTTATAATAATTTTACGTCTCTTCCCAGCTTTGTGAGCGAGCACATTGAGACTCTTGAGGAGATAGATATGGAGTATAAGCACTTGGCACTTGAATCTGGGATCGAAAACTGGGGTCGTGTGCCTGCTCTCAATTGCAAATCTTCTTTCATTTTGGATTTGGCTGATGCAGTGATCGAAGCCCTTCCATCCGCTACAGCTTTGTCGCCCCACGCAAGCTCAACAGATGCAGATGATTGTGATCCTTTCCAATATGCTATCAAATTGCTATTTGGTTCAGTCCTCGCAATAATCTTGTTACTTTCACCAAAAGCATTTCTGGCTTTTAGGAATAATTTCATTTAG

mRNA sequence

ATGGACGCGACCTCTTCCTCTGGCGCTCTTTCTAATATGAAGCTTCTCAGTTCCAATAAACTCAATTCGGATCACAGGGTTCCCACGTTATTCTCCTTGCCGAAACCTCTCGTGTCTTTATCTTGCAAATCCTATAGAAATTTGCAAGTTCGTGATAAGTCTACATCTCAAGGATTAGTGGTTTCTTGTTCTAGCTCTAATAGTCACAGAGATGTGATGCAGGGGTTTCACCTTTCTGGCCCCATTGAGAAGAGAAGCCGTCTTGGACAAGCATTTTGCTCGGTAGAAAGTTTCACCTATGGTGAATATGCTTTAGAATCTCGCTCTCAGGCAGCGGAAGAGAAGGTTGGAGTTCTGCTTCTGAATCTAGGAGGGCCAGACACGCTTGATGATGTTCAGCCATTTCTGTACAATCTATTTGCAGATCCGGATATTATCCGGCTCCCAAGGTTGTTCCGTTTCCTCCAAGAGCCATTGGCCAAATTAATATCTACCTTTCGTGCTCCCAAAAGTAAAGAAGGGTATGCTTCAATAGGAGGCGGCTCACCTTTGCGTAAAATAACAGATGAGCAGGCACAGGCGCTTAAAATGGCTTTGGAAGAGAAAAACACACCTGCTAATGTCTATGTGGGAATGCGCTATTGGTACCCTTTCACAGAGGAAGCAATTCAGCAAATTAAGAGGGATGGGATAACAAGACTTGTAGTGCTGCCATTATATCCACAGTACTCTATTTCCACAACTGGGTCTAGTATTCGTGTTCTCCAGAAGATTTTCAGGGAAGATGCATATCTCTCAAAATTGCCTGTTTCAGTTATAAAGTCCTGGTATCAACGTGAAGGGTATATTAAGTCCATGGCTGACTTGGTTCAGGCAGAGCTGAAGAATTTTATCAATCCTCAAGAGGTCATGATTTTCTTTAGTGCACATGGGGTTCCCGTCAGTTATGTTGAGAATGCTGGAGATCCATACAAAGATCAAATGGAGGAGTGCATCTACCTAATCATGCAAGAGCTGAAAGCCAGAGGAATTGGAAATGAGCATACTCTTGCTTATCAGAGCCGAGTTGGACCTGTACAATGGCTGAAGCCATACACAGATGAAGTTCTTGTTGAGCTTGGCCAGAAGGGTATTAAGAGTCTCCTAGCCGTTCCTGTGAGCTTTGTGAGCGAGCACATTGAGACTCTTGAGGAGATAGATATGGAGTATAAGCACTTGGCACTTGAATCTGGGATCGAAAACTGGGGTCGTGTGCCTGCTCTCAATTGCAAATCTTCTTTCATTTTGGATTTGGCTGATGCAGTGATCGAAGCCCTTCCATCCGCTACAGCTTTGTCGCCCCACGCAAGCTCAACAGATGCAGATGATTGTGATCCTTTCCAATATGCTATCAAATTGCTATTTGGTTCAGTCCTCGCAATAATCTTGTTACTTTCACCAAAAGCATTTCTGGCTTTTAGGAATAATTTCATTTAG

Coding sequence (CDS)

ATGGACGCGACCTCTTCCTCTGGCGCTCTTTCTAATATGAAGCTTCTCAGTTCCAATAAACTCAATTCGGATCACAGGGTTCCCACGTTATTCTCCTTGCCGAAACCTCTCGTGTCTTTATCTTGCAAATCCTATAGAAATTTGCAAGTTCGTGATAAGTCTACATCTCAAGGATTAGTGGTTTCTTGTTCTAGCTCTAATAGTCACAGAGATGTGATGCAGGGGTTTCACCTTTCTGGCCCCATTGAGAAGAGAAGCCGTCTTGGACAAGCATTTTGCTCGGTAGAAAGTTTCACCTATGGTGAATATGCTTTAGAATCTCGCTCTCAGGCAGCGGAAGAGAAGGTTGGAGTTCTGCTTCTGAATCTAGGAGGGCCAGACACGCTTGATGATGTTCAGCCATTTCTGTACAATCTATTTGCAGATCCGGATATTATCCGGCTCCCAAGGTTGTTCCGTTTCCTCCAAGAGCCATTGGCCAAATTAATATCTACCTTTCGTGCTCCCAAAAGTAAAGAAGGGTATGCTTCAATAGGAGGCGGCTCACCTTTGCGTAAAATAACAGATGAGCAGGCACAGGCGCTTAAAATGGCTTTGGAAGAGAAAAACACACCTGCTAATGTCTATGTGGGAATGCGCTATTGGTACCCTTTCACAGAGGAAGCAATTCAGCAAATTAAGAGGGATGGGATAACAAGACTTGTAGTGCTGCCATTATATCCACAGTACTCTATTTCCACAACTGGGTCTAGTATTCGTGTTCTCCAGAAGATTTTCAGGGAAGATGCATATCTCTCAAAATTGCCTGTTTCAGTTATAAAGTCCTGGTATCAACGTGAAGGGTATATTAAGTCCATGGCTGACTTGGTTCAGGCAGAGCTGAAGAATTTTATCAATCCTCAAGAGGTCATGATTTTCTTTAGTGCACATGGGGTTCCCGTCAGTTATGTTGAGAATGCTGGAGATCCATACAAAGATCAAATGGAGGAGTGCATCTACCTAATCATGCAAGAGCTGAAAGCCAGAGGAATTGGAAATGAGCATACTCTTGCTTATCAGAGCCGAGTTGGACCTGTACAATGGCTGAAGCCATACACAGATGAAGTTCTTGTTGAGCTTGGCCAGAAGGGTATTAAGAGTCTCCTAGCCGTTCCTGTGAGCTTTGTGAGCGAGCACATTGAGACTCTTGAGGAGATAGATATGGAGTATAAGCACTTGGCACTTGAATCTGGGATCGAAAACTGGGGTCGTGTGCCTGCTCTCAATTGCAAATCTTCTTTCATTTTGGATTTGGCTGATGCAGTGATCGAAGCCCTTCCATCCGCTACAGCTTTGTCGCCCCACGCAAGCTCAACAGATGCAGATGATTGTGATCCTTTCCAATATGCTATCAAATTGCTATTTGGTTCAGTCCTCGCAATAATCTTGTTACTTTCACCAAAAGCATTTCTGGCTTTTAGGAATAATTTCATTTAG

Protein sequence

MDATSSSGALSNMKLLSSNKLNSDHRVPTLFSLPKPLVSLSCKSYRNLQVRDKSTSQGLVVSCSSSNSHRDVMQGFHLSGPIEKRSRLGQAFCSVESFTYGEYALESRSQAAEEKVGVLLLNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNTPANVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLYPQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWYQREGYIKSMADLVQAELKNFINPQEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPALNCKSSFILDLADAVIEALPSATALSPHASSTDADDCDPFQYAIKLLFGSVLAIILLLSPKAFLAFRNNFI
Homology
BLAST of Csor.00g166880 vs. ExPASy Swiss-Prot
Match: P42044 (Ferrochelatase-2, chloroplastic OS=Cucumis sativus OX=3659 GN=HEMH PE=2 SV=1)

HSP 1 Score: 845.5 bits (2183), Expect = 3.0e-244
Identity = 433/492 (88.01%), Postives = 456/492 (92.68%), Query Frame = 0

Query: 1   MDATSSSGALSNMKLL-SSNKLNSDHRVPTLFSLPKPLVSLSCKSYRNLQVRDKSTSQGL 60
           MDA SSS ALSN+KL  S+N LNSD R+ +L SLPK  V+ SCK+  NLQVRD+ST  GL
Sbjct: 1   MDAASSSLALSNIKLHGSTNTLNSDQRISSLCSLPKSRVTFSCKTSGNLQVRDRST--GL 60

Query: 61  VVSCSSSNSHRDVMQGFHLSGPIEKRSRLGQAFCSVESFTYGEYALESRSQAAEEKVGVL 120
           VVSCSSSN  RDV+QG HLSGPIEK+SRLGQA CSV +FT GE+ALES+SQA ++KVGVL
Sbjct: 61  VVSCSSSNGDRDVIQGLHLSGPIEKKSRLGQACCSVGTFTVGEFALESQSQAVDDKVGVL 120

Query: 121 LLNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIG 180
           LLNLGGP+TLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLIST+RAPKSKEGYASIG
Sbjct: 121 LLNLGGPETLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTYRAPKSKEGYASIG 180

Query: 181 GGSPLRKITDEQAQALKMALEEKNTPANVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPL 240
           GGSPLRKITDEQAQALKMAL EKN   NVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPL
Sbjct: 181 GGSPLRKITDEQAQALKMALAEKNMSTNVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPL 240

Query: 241 YPQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWYQREGYIKSMADLVQAELKNFIN 300
           YPQYSISTTGSSIRVLQK+FREDAYLS LPVS+IKSWYQREGYIKSMADL+QAELKNF N
Sbjct: 241 YPQYSISTTGSSIRVLQKMFREDAYLSSLPVSIIKSWYQREGYIKSMADLMQAELKNFAN 300

Query: 301 PQEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKARGIGNEHTLAYQSRVGPV 360
           PQEVMIFFSAHGVPVSYVENAGDPYKDQMEECI LIMQELKARGIGNEHTLAYQSRVGPV
Sbjct: 301 PQEVMIFFSAHGVPVSYVENAGDPYKDQMEECICLIMQELKARGIGNEHTLAYQSRVGPV 360

Query: 361 QWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVP 420
           QWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGI+NWGRVP
Sbjct: 361 QWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIQNWGRVP 420

Query: 421 ALNCKSSFILDLADAVIEALPSATALSPHASSTDADDCDPFQYAIKLLFGSVLAIILLLS 480
           ALNC SSFI DLADAVIEALPSATAL+PH SSTDADD DPF YAIKLLFGSVLA ILLLS
Sbjct: 421 ALNCNSSFISDLADAVIEALPSATALAPHTSSTDADDHDPFLYAIKLLFGSVLAFILLLS 480

Query: 481 PKAFLAFRNNFI 492
           PKAF+ FRNNF+
Sbjct: 481 PKAFMVFRNNFL 490

BLAST of Csor.00g166880 vs. ExPASy Swiss-Prot
Match: Q69TB1 (Ferrochelatase-1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0297000 PE=2 SV=1)

HSP 1 Score: 615.9 bits (1587), Expect = 3.9e-175
Identity = 308/416 (74.04%), Postives = 349/416 (83.89%), Query Frame = 0

Query: 77  HLSGPIEKRSRLGQAFCSVESF-TYGEYALESRSQAAEEKVGVLLLNLGGPDTLDDVQPF 136
           H S  +++R  L     S + + T+ E      S AAEEKVGVLLLNLGGP+TLDDVQPF
Sbjct: 67  HRSPVLKQRQNLSARSTSADVYTTFDENVRAVSSHAAEEKVGVLLLNLGGPETLDDVQPF 126

Query: 137 LYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGGGSPLRKITDEQAQAL 196
           L+NLFADPDIIRLPRLFRFLQ PLAKLISTFRAPKSKEGYASIGGGSPLRKITDEQA AL
Sbjct: 127 LFNLFADPDIIRLPRLFRFLQRPLAKLISTFRAPKSKEGYASIGGGSPLRKITDEQANAL 186

Query: 197 KMALEEKNTPANVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLYPQYSISTTGSSIRVL 256
           K+AL++KN  AN+YVGMRYWYPFTEEAI QIK+D IT+LVVLPLYPQYSIST+GSSIRVL
Sbjct: 187 KVALKKKNLNANIYVGMRYWYPFTEEAIDQIKKDKITKLVVLPLYPQYSISTSGSSIRVL 246

Query: 257 QKIFREDAYLSKLPVSVIKSWYQREGYIKSMADLVQAELKNFINPQEVMIFFSAHGVPVS 316
           Q I +ED+Y + LP+S+I+SWYQR+GY+KSMADL++ EL  F NP+EVMIFFSAHGVP++
Sbjct: 247 QNIVKEDSYFAGLPISIIESWYQRDGYVKSMADLIEKELSIFSNPEEVMIFFSAHGVPLT 306

Query: 317 YVENAGDPYKDQMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQ 376
           YV +AGDPY+DQME+CI LIM ELK+RGI N HTLAYQSRVGPVQWLKPYTDEVLVELGQ
Sbjct: 307 YVTDAGDPYRDQMEDCIALIMGELKSRGILNSHTLAYQSRVGPVQWLKPYTDEVLVELGQ 366

Query: 377 KGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPALNCKSSFILDLADAV 436
           +G+KSLLAVPVSFVSEHIETLEEIDMEYK LALESGIENWGRVPAL C SSFI DLADAV
Sbjct: 367 QGVKSLLAVPVSFVSEHIETLEEIDMEYKELALESGIENWGRVPALGCTSSFISDLADAV 426

Query: 437 IEALPSATALSPHASSTDADDCDPFQYAIKLLFGSVLAIILLLSPKAFLAFRNNFI 492
           +EALPSA+AL          D D   Y  K+ FGS+LA +LLLSP+   AFRN  +
Sbjct: 427 VEALPSASALVTKKVDESDSDMDLMHYLSKMFFGSILAFVLLLSPRLISAFRNTLL 482

BLAST of Csor.00g166880 vs. ExPASy Swiss-Prot
Match: P42045 (Ferrochelatase-2, chloroplastic OS=Hordeum vulgare OX=4513 GN=HEMH PE=2 SV=1)

HSP 1 Score: 603.2 bits (1554), Expect = 2.6e-171
Identity = 298/390 (76.41%), Postives = 335/390 (85.90%), Query Frame = 0

Query: 99  TYGEYALESRSQAAEEKVGVLLLNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEP 158
           T+ E      S A EEKVGVLLLNLGGP+TL+DVQPFL+NLFADPDIIRLPRLFRFLQ P
Sbjct: 92  TFDEDVKGVSSHAVEEKVGVLLLNLGGPETLNDVQPFLFNLFADPDIIRLPRLFRFLQRP 151

Query: 159 LAKLISTFRAPKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNTPANVYVGMRYWYPF 218
           LAKLISTFRAPKS EGYASIGGGSPLRKITDEQA ALK+AL+ KN  A++YVGMRYWYPF
Sbjct: 152 LAKLISTFRAPKSNEGYASIGGGSPLRKITDEQANALKVALKSKNLEADIYVGMRYWYPF 211

Query: 219 TEEAIQQIKRDGITRLVVLPLYPQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWYQ 278
           TEEAI QIK+D IT+LVVLPLYPQYSIST+GSSIRVLQ I +ED Y + LP+S+I+SWYQ
Sbjct: 212 TEEAIDQIKKDKITKLVVLPLYPQYSISTSGSSIRVLQNIVKEDPYFAGLPISIIESWYQ 271

Query: 279 REGYIKSMADLVQAELKNFINPQEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQE 338
           REGY+KSMADL++ EL  F NP+EVMIFFSAHGVP++YV++AGDPY+DQME+CI LIM+E
Sbjct: 272 REGYVKSMADLIEKELSVFSNPEEVMIFFSAHGVPLTYVKDAGDPYRDQMEDCIALIMEE 331

Query: 339 LKARGIGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEE 398
           LK+RG  N+HTLAYQSRVGPVQWLKPYTDEVLVELGQKG+KSLLAVPVSFVSEHIETLEE
Sbjct: 332 LKSRGTLNDHTLAYQSRVGPVQWLKPYTDEVLVELGQKGVKSLLAVPVSFVSEHIETLEE 391

Query: 399 IDMEYKHLALESGIENWGRVPALNCKSSFILDLADAVIEALPSATALSPHASSTDADDCD 458
           IDMEY+ LALESGIENWGRVPAL C SSFI DLADAV+EALPSA+A++         D D
Sbjct: 392 IDMEYRELALESGIENWGRVPALGCTSSFISDLADAVVEALPSASAMATRKVKDTDSDMD 451

Query: 459 PFQYAIKLLFGSVLAIILLLSPKAFLAFRN 489
              Y  K+  GSVLA  LLLSP+   AFRN
Sbjct: 452 MMHYLTKMFLGSVLAFFLLLSPRLVSAFRN 481

BLAST of Csor.00g166880 vs. ExPASy Swiss-Prot
Match: P42043 (Ferrochelatase-1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FC1 PE=2 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 8.4e-170
Identity = 297/394 (75.38%), Postives = 343/394 (87.06%), Query Frame = 0

Query: 99  TYGEYALESRSQ-AAEEKVGVLLLNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQE 158
           +Y E + ++RS   AE+K+GVLLLNLGGP+TL+DVQPFLYNLFADPDIIRLPR F+FLQ 
Sbjct: 72  SYDETSAKARSHVVAEDKIGVLLLNLGGPETLNDVQPFLYNLFADPDIIRLPRPFQFLQG 131

Query: 159 PLAKLISTFRAPKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNTPANVYVGMRYWYP 218
            +AK IS  RAPKSKEGYA+IGGGSPLRKITDEQA A+KM+L+ KN  ANVYVGMRYWYP
Sbjct: 132 TIAKFISVVRAPKSKEGYAAIGGGSPLRKITDEQADAIKMSLQAKNIAANVYVGMRYWYP 191

Query: 219 FTEEAIQQIKRDGITRLVVLPLYPQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWY 278
           FTEEA+QQIK+D ITRLVVLPLYPQYSISTTGSSIRVLQ +FR+D YL+ +PV++IKSWY
Sbjct: 192 FTEEAVQQIKKDKITRLVVLPLYPQYSISTTGSSIRVLQDLFRKDPYLAGVPVAIIKSWY 251

Query: 279 QREGYIKSMADLVQAELKNFINPQEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQ 338
           QR GY+ SMADL++ EL+ F +P+EVMIFFSAHGVPVSYVENAGDPY+ QMEECI LIM+
Sbjct: 252 QRRGYVNSMADLIEKELQTFSDPKEVMIFFSAHGVPVSYVENAGDPYQKQMEECIDLIME 311

Query: 339 ELKARGIGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLE 398
           ELKARG+ N+H LAYQSRVGPVQWLKPYTDEVLV+LG+ G+KSLLAVPVSFVSEHIETLE
Sbjct: 312 ELKARGVLNDHKLAYQSRVGPVQWLKPYTDEVLVDLGKSGVKSLLAVPVSFVSEHIETLE 371

Query: 399 EIDMEYKHLALESGIENWGRVPALNCKSSFILDLADAVIEALPSATALS-PHA--SSTDA 458
           EIDMEY+ LALESG+ENWGRVPAL    SFI DLADAVIE+LPSA A+S P+A   S D+
Sbjct: 372 EIDMEYRELALESGVENWGRVPALGLTPSFITDLADAVIESLPSAEAMSNPNAVVDSEDS 431

Query: 459 DDCDPFQYAIKLLFGSVLAIILLLSPKAFLAFRN 489
           +  D F Y +K+ FGS+LA +LLLSPK F AFRN
Sbjct: 432 ESSDAFSYIVKMFFGSILAFVLLLSPKMFHAFRN 465

BLAST of Csor.00g166880 vs. ExPASy Swiss-Prot
Match: Q0DIV0 (Ferrochelatase-2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0361200 PE=2 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 1.1e-148
Identity = 253/333 (75.98%), Postives = 298/333 (89.49%), Query Frame = 0

Query: 114 EKVGVLLLNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKE 173
           EK+GVLLLNLGGP+TLDDVQPFL+NLFADPDIIRLPRLFRFLQ+PLA+ IS  RAPKSKE
Sbjct: 112 EKIGVLLLNLGGPETLDDVQPFLFNLFADPDIIRLPRLFRFLQKPLAQFISVVRAPKSKE 171

Query: 174 GYASIGGGSPLRKITDEQAQALKMALEEKNTPANVYVGMRYWYPFTEEAIQQIKRDGITR 233
           GYASIGGGSPLR+ITD QA+AL+ AL +K+ PA VYVGMRYW+PFTEEAI+QIKRDGIT+
Sbjct: 172 GYASIGGGSPLRQITDAQAEALRKALCDKDIPAKVYVGMRYWHPFTEEAIEQIKRDGITK 231

Query: 234 LVVLPLYPQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWYQREGYIKSMADLVQAE 293
           LVVLPLYPQ+SIST+GSS+R+L+ IFRED YL  +  +VI SWYQREGYIK+MA L++ E
Sbjct: 232 LVVLPLYPQFSISTSGSSLRLLEGIFREDEYLVNMQHTVIPSWYQREGYIKAMATLIEKE 291

Query: 294 LKNFINPQEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKARGIGNEHTLAYQ 353
           L+ F  PQ+VMIFFSAHGVP++YVE AGDPYK +MEEC+ LIM+EL+ RGI N  TLAYQ
Sbjct: 292 LRTFSEPQKVMIFFSAHGVPLAYVEEAGDPYKAEMEECVDLIMEELEKRGITNSCTLAYQ 351

Query: 354 SRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIE 413
           SRVGPV+WL+PYTDE ++ELGQKG+KSLLAVP+SFVSEHIETLEEID+EYK LALESGI+
Sbjct: 352 SRVGPVEWLRPYTDETIIELGQKGVKSLLAVPISFVSEHIETLEEIDVEYKELALESGIK 411

Query: 414 NWGRVPALNCKSSFILDLADAVIEALPSATALS 447
           +WGRVPAL C+ +FI DLADAVIE+LP   A++
Sbjct: 412 HWGRVPALGCEPTFITDLADAVIESLPYVGAMA 444

BLAST of Csor.00g166880 vs. NCBI nr
Match: KAG6598300.1 (Ferrochelatase-2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029273.1 Ferrochelatase-2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 967 bits (2501), Expect = 0.0
Identity = 491/491 (100.00%), Postives = 491/491 (100.00%), Query Frame = 0

Query: 1   MDATSSSGALSNMKLLSSNKLNSDHRVPTLFSLPKPLVSLSCKSYRNLQVRDKSTSQGLV 60
           MDATSSSGALSNMKLLSSNKLNSDHRVPTLFSLPKPLVSLSCKSYRNLQVRDKSTSQGLV
Sbjct: 1   MDATSSSGALSNMKLLSSNKLNSDHRVPTLFSLPKPLVSLSCKSYRNLQVRDKSTSQGLV 60

Query: 61  VSCSSSNSHRDVMQGFHLSGPIEKRSRLGQAFCSVESFTYGEYALESRSQAAEEKVGVLL 120
           VSCSSSNSHRDVMQGFHLSGPIEKRSRLGQAFCSVESFTYGEYALESRSQAAEEKVGVLL
Sbjct: 61  VSCSSSNSHRDVMQGFHLSGPIEKRSRLGQAFCSVESFTYGEYALESRSQAAEEKVGVLL 120

Query: 121 LNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGG 180
           LNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGG
Sbjct: 121 LNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGG 180

Query: 181 GSPLRKITDEQAQALKMALEEKNTPANVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLY 240
           GSPLRKITDEQAQALKMALEEKNTPANVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLY
Sbjct: 181 GSPLRKITDEQAQALKMALEEKNTPANVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLY 240

Query: 241 PQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWYQREGYIKSMADLVQAELKNFINP 300
           PQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWYQREGYIKSMADLVQAELKNFINP
Sbjct: 241 PQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWYQREGYIKSMADLVQAELKNFINP 300

Query: 301 QEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQ 360
           QEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQ
Sbjct: 301 QEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQ 360

Query: 361 WLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPA 420
           WLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPA
Sbjct: 361 WLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPA 420

Query: 421 LNCKSSFILDLADAVIEALPSATALSPHASSTDADDCDPFQYAIKLLFGSVLAIILLLSP 480
           LNCKSSFILDLADAVIEALPSATALSPHASSTDADDCDPFQYAIKLLFGSVLAIILLLSP
Sbjct: 421 LNCKSSFILDLADAVIEALPSATALSPHASSTDADDCDPFQYAIKLLFGSVLAIILLLSP 480

Query: 481 KAFLAFRNNFI 491
           KAFLAFRNNFI
Sbjct: 481 KAFLAFRNNFI 491

BLAST of Csor.00g166880 vs. NCBI nr
Match: XP_022962468.1 (ferrochelatase-2, chloroplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 961 bits (2485), Expect = 0.0
Identity = 488/491 (99.39%), Postives = 490/491 (99.80%), Query Frame = 0

Query: 1   MDATSSSGALSNMKLLSSNKLNSDHRVPTLFSLPKPLVSLSCKSYRNLQVRDKSTSQGLV 60
           MDATSSSGALSNMKLLS+NKLNSDHRVPTLFSLPKPLVSLSCKSYRNLQVRDKSTSQGLV
Sbjct: 1   MDATSSSGALSNMKLLSANKLNSDHRVPTLFSLPKPLVSLSCKSYRNLQVRDKSTSQGLV 60

Query: 61  VSCSSSNSHRDVMQGFHLSGPIEKRSRLGQAFCSVESFTYGEYALESRSQAAEEKVGVLL 120
           VSCSSSNSHRDVMQGFHLSGPIEKRSRLGQAFCSVESFTYGEYALESRSQAAEEKVGVLL
Sbjct: 61  VSCSSSNSHRDVMQGFHLSGPIEKRSRLGQAFCSVESFTYGEYALESRSQAAEEKVGVLL 120

Query: 121 LNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGG 180
           LNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGG
Sbjct: 121 LNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGG 180

Query: 181 GSPLRKITDEQAQALKMALEEKNTPANVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLY 240
           GSPLRKITDEQAQALKMALEEKNT ANVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLY
Sbjct: 181 GSPLRKITDEQAQALKMALEEKNTSANVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLY 240

Query: 241 PQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWYQREGYIKSMADLVQAELKNFINP 300
           PQYSISTTGSSIRVLQKIFREDA+LSKLPVSVIKSWYQREGYIKSMADLVQAELKNFINP
Sbjct: 241 PQYSISTTGSSIRVLQKIFREDAHLSKLPVSVIKSWYQREGYIKSMADLVQAELKNFINP 300

Query: 301 QEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQ 360
           QEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQ
Sbjct: 301 QEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQ 360

Query: 361 WLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPA 420
           WLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPA
Sbjct: 361 WLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPA 420

Query: 421 LNCKSSFILDLADAVIEALPSATALSPHASSTDADDCDPFQYAIKLLFGSVLAIILLLSP 480
           LNCKSSFILDLADAVIEALPSATALSPHASSTDADDCDPFQYAIKLLFGSVLAIILLLSP
Sbjct: 421 LNCKSSFILDLADAVIEALPSATALSPHASSTDADDCDPFQYAIKLLFGSVLAIILLLSP 480

Query: 481 KAFLAFRNNFI 491
           KAFLAFRNNFI
Sbjct: 481 KAFLAFRNNFI 491

BLAST of Csor.00g166880 vs. NCBI nr
Match: XP_023546547.1 (ferrochelatase-2, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 961 bits (2483), Expect = 0.0
Identity = 488/491 (99.39%), Postives = 489/491 (99.59%), Query Frame = 0

Query: 1   MDATSSSGALSNMKLLSSNKLNSDHRVPTLFSLPKPLVSLSCKSYRNLQVRDKSTSQGLV 60
           MDATSSSGALSNMKLLSSNKLNSDHRVP+LFSLPKPLVSLSCKSYRNLQVRDKSTSQGLV
Sbjct: 1   MDATSSSGALSNMKLLSSNKLNSDHRVPSLFSLPKPLVSLSCKSYRNLQVRDKSTSQGLV 60

Query: 61  VSCSSSNSHRDVMQGFHLSGPIEKRSRLGQAFCSVESFTYGEYALESRSQAAEEKVGVLL 120
           VSCSSSNSHRDVMQGFHLSGP EKRSRLGQAFCSVESFTYGEYALESRSQAAEEKVGVLL
Sbjct: 61  VSCSSSNSHRDVMQGFHLSGPSEKRSRLGQAFCSVESFTYGEYALESRSQAAEEKVGVLL 120

Query: 121 LNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGG 180
           LNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGG
Sbjct: 121 LNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGG 180

Query: 181 GSPLRKITDEQAQALKMALEEKNTPANVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLY 240
           GSPLRKITDEQAQALKMALEEKNT ANVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLY
Sbjct: 181 GSPLRKITDEQAQALKMALEEKNTSANVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLY 240

Query: 241 PQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWYQREGYIKSMADLVQAELKNFINP 300
           PQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWYQREGYIKSMADLVQAELKNFINP
Sbjct: 241 PQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWYQREGYIKSMADLVQAELKNFINP 300

Query: 301 QEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQ 360
           QEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQ
Sbjct: 301 QEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQ 360

Query: 361 WLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPA 420
           WLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPA
Sbjct: 361 WLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPA 420

Query: 421 LNCKSSFILDLADAVIEALPSATALSPHASSTDADDCDPFQYAIKLLFGSVLAIILLLSP 480
           LNCKSSFILDLADAVIEALPSATALSPHASSTDADDCDPFQYAIKLLFGSVLAIILLLSP
Sbjct: 421 LNCKSSFILDLADAVIEALPSATALSPHASSTDADDCDPFQYAIKLLFGSVLAIILLLSP 480

Query: 481 KAFLAFRNNFI 491
           KAFLAFRNNFI
Sbjct: 481 KAFLAFRNNFI 491

BLAST of Csor.00g166880 vs. NCBI nr
Match: XP_022997506.1 (ferrochelatase-2, chloroplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 951 bits (2458), Expect = 0.0
Identity = 485/491 (98.78%), Postives = 486/491 (98.98%), Query Frame = 0

Query: 1   MDATSSSGALSNMKLLSSNKLNSDHRVPTLFSLPKPLVSLSCKSYRNLQVRDKSTSQGLV 60
           MDATSSSGALSN+KLLSSNKLNSDHRVPTLFSLPKPLVSLSCKSYRNLQVRDKSTSQGLV
Sbjct: 1   MDATSSSGALSNVKLLSSNKLNSDHRVPTLFSLPKPLVSLSCKSYRNLQVRDKSTSQGLV 60

Query: 61  VSCSSSNSHRDVMQGFHLSGPIEKRSRLGQAFCSVESFTYGEYALESRSQAAEEKVGVLL 120
           VSCSSS SHRDVMQG HLSGPIEKRSRLGQAFCSV SFT GEYALESRSQAAEEKVGVLL
Sbjct: 61  VSCSSSISHRDVMQGLHLSGPIEKRSRLGQAFCSVGSFTCGEYALESRSQAAEEKVGVLL 120

Query: 121 LNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGG 180
           LNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGG
Sbjct: 121 LNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGG 180

Query: 181 GSPLRKITDEQAQALKMALEEKNTPANVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLY 240
           GSPLRKITDEQAQALKMALEEKNT ANVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLY
Sbjct: 181 GSPLRKITDEQAQALKMALEEKNTSANVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLY 240

Query: 241 PQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWYQREGYIKSMADLVQAELKNFINP 300
           PQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWYQREGYIKSMADLVQAELKNFINP
Sbjct: 241 PQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWYQREGYIKSMADLVQAELKNFINP 300

Query: 301 QEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQ 360
           QEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQ
Sbjct: 301 QEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQ 360

Query: 361 WLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPA 420
           WLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPA
Sbjct: 361 WLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPA 420

Query: 421 LNCKSSFILDLADAVIEALPSATALSPHASSTDADDCDPFQYAIKLLFGSVLAIILLLSP 480
           LNCKSSFILDLADAVIEALPSATALSPHASSTDADDCDPFQYAIKLLFGSVLAIILLLSP
Sbjct: 421 LNCKSSFILDLADAVIEALPSATALSPHASSTDADDCDPFQYAIKLLFGSVLAIILLLSP 480

Query: 481 KAFLAFRNNFI 491
           KAFLAFRNNFI
Sbjct: 481 KAFLAFRNNFI 491

BLAST of Csor.00g166880 vs. NCBI nr
Match: XP_022962470.1 (ferrochelatase-2, chloroplastic isoform X2 [Cucurbita moschata])

HSP 1 Score: 914 bits (2361), Expect = 0.0
Identity = 461/465 (99.14%), Postives = 463/465 (99.57%), Query Frame = 0

Query: 27  VPTLFSLPKPLVSLSCKSYRNLQVRDKSTSQGLVVSCSSSNSHRDVMQGFHLSGPIEKRS 86
           +P LFSLPKPLVSLSCKSYRNLQVRDKSTSQGLVVSCSSSNSHRDVMQGFHLSGPIEKRS
Sbjct: 1   MPRLFSLPKPLVSLSCKSYRNLQVRDKSTSQGLVVSCSSSNSHRDVMQGFHLSGPIEKRS 60

Query: 87  RLGQAFCSVESFTYGEYALESRSQAAEEKVGVLLLNLGGPDTLDDVQPFLYNLFADPDII 146
           RLGQAFCSVESFTYGEYALESRSQAAEEKVGVLLLNLGGPDTLDDVQPFLYNLFADPDII
Sbjct: 61  RLGQAFCSVESFTYGEYALESRSQAAEEKVGVLLLNLGGPDTLDDVQPFLYNLFADPDII 120

Query: 147 RLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNTPA 206
           RLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNT A
Sbjct: 121 RLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNTSA 180

Query: 207 NVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLYPQYSISTTGSSIRVLQKIFREDAYLS 266
           NVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLYPQYSISTTGSSIRVLQKIFREDA+LS
Sbjct: 181 NVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLYPQYSISTTGSSIRVLQKIFREDAHLS 240

Query: 267 KLPVSVIKSWYQREGYIKSMADLVQAELKNFINPQEVMIFFSAHGVPVSYVENAGDPYKD 326
           KLPVSVIKSWYQREGYIKSMADLVQAELKNFINPQEVMIFFSAHGVPVSYVENAGDPYKD
Sbjct: 241 KLPVSVIKSWYQREGYIKSMADLVQAELKNFINPQEVMIFFSAHGVPVSYVENAGDPYKD 300

Query: 327 QMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPV 386
           QMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPV
Sbjct: 301 QMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPV 360

Query: 387 SFVSEHIETLEEIDMEYKHLALESGIENWGRVPALNCKSSFILDLADAVIEALPSATALS 446
           SFVSEHIETLEEIDMEYKHLALESGIENWGRVPALNCKSSFILDLADAVIEALPSATALS
Sbjct: 361 SFVSEHIETLEEIDMEYKHLALESGIENWGRVPALNCKSSFILDLADAVIEALPSATALS 420

Query: 447 PHASSTDADDCDPFQYAIKLLFGSVLAIILLLSPKAFLAFRNNFI 491
           PHASSTDADDCDPFQYAIKLLFGSVLAIILLLSPKAFLAFRNNFI
Sbjct: 421 PHASSTDADDCDPFQYAIKLLFGSVLAIILLLSPKAFLAFRNNFI 465

BLAST of Csor.00g166880 vs. ExPASy TrEMBL
Match: A0A6J1HEW3 (Ferrochelatase OS=Cucurbita moschata OX=3662 GN=LOC111462897 PE=3 SV=1)

HSP 1 Score: 961 bits (2485), Expect = 0.0
Identity = 488/491 (99.39%), Postives = 490/491 (99.80%), Query Frame = 0

Query: 1   MDATSSSGALSNMKLLSSNKLNSDHRVPTLFSLPKPLVSLSCKSYRNLQVRDKSTSQGLV 60
           MDATSSSGALSNMKLLS+NKLNSDHRVPTLFSLPKPLVSLSCKSYRNLQVRDKSTSQGLV
Sbjct: 1   MDATSSSGALSNMKLLSANKLNSDHRVPTLFSLPKPLVSLSCKSYRNLQVRDKSTSQGLV 60

Query: 61  VSCSSSNSHRDVMQGFHLSGPIEKRSRLGQAFCSVESFTYGEYALESRSQAAEEKVGVLL 120
           VSCSSSNSHRDVMQGFHLSGPIEKRSRLGQAFCSVESFTYGEYALESRSQAAEEKVGVLL
Sbjct: 61  VSCSSSNSHRDVMQGFHLSGPIEKRSRLGQAFCSVESFTYGEYALESRSQAAEEKVGVLL 120

Query: 121 LNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGG 180
           LNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGG
Sbjct: 121 LNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGG 180

Query: 181 GSPLRKITDEQAQALKMALEEKNTPANVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLY 240
           GSPLRKITDEQAQALKMALEEKNT ANVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLY
Sbjct: 181 GSPLRKITDEQAQALKMALEEKNTSANVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLY 240

Query: 241 PQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWYQREGYIKSMADLVQAELKNFINP 300
           PQYSISTTGSSIRVLQKIFREDA+LSKLPVSVIKSWYQREGYIKSMADLVQAELKNFINP
Sbjct: 241 PQYSISTTGSSIRVLQKIFREDAHLSKLPVSVIKSWYQREGYIKSMADLVQAELKNFINP 300

Query: 301 QEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQ 360
           QEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQ
Sbjct: 301 QEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQ 360

Query: 361 WLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPA 420
           WLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPA
Sbjct: 361 WLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPA 420

Query: 421 LNCKSSFILDLADAVIEALPSATALSPHASSTDADDCDPFQYAIKLLFGSVLAIILLLSP 480
           LNCKSSFILDLADAVIEALPSATALSPHASSTDADDCDPFQYAIKLLFGSVLAIILLLSP
Sbjct: 421 LNCKSSFILDLADAVIEALPSATALSPHASSTDADDCDPFQYAIKLLFGSVLAIILLLSP 480

Query: 481 KAFLAFRNNFI 491
           KAFLAFRNNFI
Sbjct: 481 KAFLAFRNNFI 491

BLAST of Csor.00g166880 vs. ExPASy TrEMBL
Match: A0A6J1K7N7 (Ferrochelatase OS=Cucurbita maxima OX=3661 GN=LOC111492405 PE=3 SV=1)

HSP 1 Score: 951 bits (2458), Expect = 0.0
Identity = 485/491 (98.78%), Postives = 486/491 (98.98%), Query Frame = 0

Query: 1   MDATSSSGALSNMKLLSSNKLNSDHRVPTLFSLPKPLVSLSCKSYRNLQVRDKSTSQGLV 60
           MDATSSSGALSN+KLLSSNKLNSDHRVPTLFSLPKPLVSLSCKSYRNLQVRDKSTSQGLV
Sbjct: 1   MDATSSSGALSNVKLLSSNKLNSDHRVPTLFSLPKPLVSLSCKSYRNLQVRDKSTSQGLV 60

Query: 61  VSCSSSNSHRDVMQGFHLSGPIEKRSRLGQAFCSVESFTYGEYALESRSQAAEEKVGVLL 120
           VSCSSS SHRDVMQG HLSGPIEKRSRLGQAFCSV SFT GEYALESRSQAAEEKVGVLL
Sbjct: 61  VSCSSSISHRDVMQGLHLSGPIEKRSRLGQAFCSVGSFTCGEYALESRSQAAEEKVGVLL 120

Query: 121 LNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGG 180
           LNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGG
Sbjct: 121 LNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGG 180

Query: 181 GSPLRKITDEQAQALKMALEEKNTPANVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLY 240
           GSPLRKITDEQAQALKMALEEKNT ANVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLY
Sbjct: 181 GSPLRKITDEQAQALKMALEEKNTSANVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLY 240

Query: 241 PQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWYQREGYIKSMADLVQAELKNFINP 300
           PQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWYQREGYIKSMADLVQAELKNFINP
Sbjct: 241 PQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWYQREGYIKSMADLVQAELKNFINP 300

Query: 301 QEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQ 360
           QEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQ
Sbjct: 301 QEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQ 360

Query: 361 WLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPA 420
           WLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPA
Sbjct: 361 WLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPA 420

Query: 421 LNCKSSFILDLADAVIEALPSATALSPHASSTDADDCDPFQYAIKLLFGSVLAIILLLSP 480
           LNCKSSFILDLADAVIEALPSATALSPHASSTDADDCDPFQYAIKLLFGSVLAIILLLSP
Sbjct: 421 LNCKSSFILDLADAVIEALPSATALSPHASSTDADDCDPFQYAIKLLFGSVLAIILLLSP 480

Query: 481 KAFLAFRNNFI 491
           KAFLAFRNNFI
Sbjct: 481 KAFLAFRNNFI 491

BLAST of Csor.00g166880 vs. ExPASy TrEMBL
Match: A0A6J1HF73 (Ferrochelatase OS=Cucurbita moschata OX=3662 GN=LOC111462897 PE=3 SV=1)

HSP 1 Score: 914 bits (2361), Expect = 0.0
Identity = 461/465 (99.14%), Postives = 463/465 (99.57%), Query Frame = 0

Query: 27  VPTLFSLPKPLVSLSCKSYRNLQVRDKSTSQGLVVSCSSSNSHRDVMQGFHLSGPIEKRS 86
           +P LFSLPKPLVSLSCKSYRNLQVRDKSTSQGLVVSCSSSNSHRDVMQGFHLSGPIEKRS
Sbjct: 1   MPRLFSLPKPLVSLSCKSYRNLQVRDKSTSQGLVVSCSSSNSHRDVMQGFHLSGPIEKRS 60

Query: 87  RLGQAFCSVESFTYGEYALESRSQAAEEKVGVLLLNLGGPDTLDDVQPFLYNLFADPDII 146
           RLGQAFCSVESFTYGEYALESRSQAAEEKVGVLLLNLGGPDTLDDVQPFLYNLFADPDII
Sbjct: 61  RLGQAFCSVESFTYGEYALESRSQAAEEKVGVLLLNLGGPDTLDDVQPFLYNLFADPDII 120

Query: 147 RLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNTPA 206
           RLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNT A
Sbjct: 121 RLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNTSA 180

Query: 207 NVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLYPQYSISTTGSSIRVLQKIFREDAYLS 266
           NVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLYPQYSISTTGSSIRVLQKIFREDA+LS
Sbjct: 181 NVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLYPQYSISTTGSSIRVLQKIFREDAHLS 240

Query: 267 KLPVSVIKSWYQREGYIKSMADLVQAELKNFINPQEVMIFFSAHGVPVSYVENAGDPYKD 326
           KLPVSVIKSWYQREGYIKSMADLVQAELKNFINPQEVMIFFSAHGVPVSYVENAGDPYKD
Sbjct: 241 KLPVSVIKSWYQREGYIKSMADLVQAELKNFINPQEVMIFFSAHGVPVSYVENAGDPYKD 300

Query: 327 QMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPV 386
           QMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPV
Sbjct: 301 QMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPV 360

Query: 387 SFVSEHIETLEEIDMEYKHLALESGIENWGRVPALNCKSSFILDLADAVIEALPSATALS 446
           SFVSEHIETLEEIDMEYKHLALESGIENWGRVPALNCKSSFILDLADAVIEALPSATALS
Sbjct: 361 SFVSEHIETLEEIDMEYKHLALESGIENWGRVPALNCKSSFILDLADAVIEALPSATALS 420

Query: 447 PHASSTDADDCDPFQYAIKLLFGSVLAIILLLSPKAFLAFRNNFI 491
           PHASSTDADDCDPFQYAIKLLFGSVLAIILLLSPKAFLAFRNNFI
Sbjct: 421 PHASSTDADDCDPFQYAIKLLFGSVLAIILLLSPKAFLAFRNNFI 465

BLAST of Csor.00g166880 vs. ExPASy TrEMBL
Match: A0A6J1K585 (Ferrochelatase OS=Cucurbita maxima OX=3661 GN=LOC111492405 PE=3 SV=1)

HSP 1 Score: 904 bits (2335), Expect = 0.0
Identity = 458/465 (98.49%), Postives = 459/465 (98.71%), Query Frame = 0

Query: 27  VPTLFSLPKPLVSLSCKSYRNLQVRDKSTSQGLVVSCSSSNSHRDVMQGFHLSGPIEKRS 86
           +P LFSLPKPLVSLSCKSYRNLQVRDKSTSQGLVVSCSSS SHRDVMQG HLSGPIEKRS
Sbjct: 1   MPRLFSLPKPLVSLSCKSYRNLQVRDKSTSQGLVVSCSSSISHRDVMQGLHLSGPIEKRS 60

Query: 87  RLGQAFCSVESFTYGEYALESRSQAAEEKVGVLLLNLGGPDTLDDVQPFLYNLFADPDII 146
           RLGQAFCSV SFT GEYALESRSQAAEEKVGVLLLNLGGPDTLDDVQPFLYNLFADPDII
Sbjct: 61  RLGQAFCSVGSFTCGEYALESRSQAAEEKVGVLLLNLGGPDTLDDVQPFLYNLFADPDII 120

Query: 147 RLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNTPA 206
           RLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNT A
Sbjct: 121 RLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNTSA 180

Query: 207 NVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLYPQYSISTTGSSIRVLQKIFREDAYLS 266
           NVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLYPQYSISTTGSSIRVLQKIFREDAYLS
Sbjct: 181 NVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLYPQYSISTTGSSIRVLQKIFREDAYLS 240

Query: 267 KLPVSVIKSWYQREGYIKSMADLVQAELKNFINPQEVMIFFSAHGVPVSYVENAGDPYKD 326
           KLPVSVIKSWYQREGYIKSMADLVQAELKNFINPQEVMIFFSAHGVPVSYVENAGDPYKD
Sbjct: 241 KLPVSVIKSWYQREGYIKSMADLVQAELKNFINPQEVMIFFSAHGVPVSYVENAGDPYKD 300

Query: 327 QMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPV 386
           QMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPV
Sbjct: 301 QMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPV 360

Query: 387 SFVSEHIETLEEIDMEYKHLALESGIENWGRVPALNCKSSFILDLADAVIEALPSATALS 446
           SFVSEHIETLEEIDMEYKHLALESGIENWGRVPALNCKSSFILDLADAVIEALPSATALS
Sbjct: 361 SFVSEHIETLEEIDMEYKHLALESGIENWGRVPALNCKSSFILDLADAVIEALPSATALS 420

Query: 447 PHASSTDADDCDPFQYAIKLLFGSVLAIILLLSPKAFLAFRNNFI 491
           PHASSTDADDCDPFQYAIKLLFGSVLAIILLLSPKAFLAFRNNFI
Sbjct: 421 PHASSTDADDCDPFQYAIKLLFGSVLAIILLLSPKAFLAFRNNFI 465

BLAST of Csor.00g166880 vs. ExPASy TrEMBL
Match: A0A5A7V5D0 (Ferrochelatase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G001260 PE=3 SV=1)

HSP 1 Score: 851 bits (2199), Expect = 1.23e-309
Identity = 434/492 (88.21%), Postives = 460/492 (93.50%), Query Frame = 0

Query: 1   MDATSSSGALSNMKLL-SSNKLNSDHRVPTLFSLPKPLVSLSCKSYRNLQVRDKSTSQGL 60
           MDA SSS ALSN+KL  S+N LNSD R+ +L SLPK  V+ SCKS  NLQV D+ST  GL
Sbjct: 1   MDAASSSLALSNIKLHGSTNTLNSDKRISSLCSLPKSGVAFSCKSSGNLQVHDRST--GL 60

Query: 61  VVSCSSSNSHRDVMQGFHLSGPIEKRSRLGQAFCSVESFTYGEYALESRSQAAEEKVGVL 120
           VVSCSSSN HRDV+QG HLSGPIEK+SRLGQA CSV +FT GE+ALES+SQA ++KVGVL
Sbjct: 61  VVSCSSSNGHRDVIQGLHLSGPIEKKSRLGQACCSVGTFTVGEFALESQSQAVDDKVGVL 120

Query: 121 LLNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIG 180
           LLNLGGP+TLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLIST+RAPKSKEGYASIG
Sbjct: 121 LLNLGGPETLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTYRAPKSKEGYASIG 180

Query: 181 GGSPLRKITDEQAQALKMALEEKNTPANVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPL 240
           GGSPLRKITDEQAQALKMALEEKN  ANVYVGMRYWYPFTEEAI+QIKRDGITRLVVLPL
Sbjct: 181 GGSPLRKITDEQAQALKMALEEKNVSANVYVGMRYWYPFTEEAIEQIKRDGITRLVVLPL 240

Query: 241 YPQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWYQREGYIKSMADLVQAELKNFIN 300
           YPQYSISTTGSSIRVLQK+FREDAYLS LPVS+IKSWYQREGYI+SMADL+QAELKNF N
Sbjct: 241 YPQYSISTTGSSIRVLQKMFREDAYLSSLPVSIIKSWYQREGYIRSMADLIQAELKNFAN 300

Query: 301 PQEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKARGIGNEHTLAYQSRVGPV 360
           P+EVMIFFSAHGVPVSYVENAGDPYKD+MEECIYLIMQELKARGIGNEHTLAYQSRVGPV
Sbjct: 301 PREVMIFFSAHGVPVSYVENAGDPYKDEMEECIYLIMQELKARGIGNEHTLAYQSRVGPV 360

Query: 361 QWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVP 420
           QWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVP
Sbjct: 361 QWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVP 420

Query: 421 ALNCKSSFILDLADAVIEALPSATALSPHASSTDADDCDPFQYAIKLLFGSVLAIILLLS 480
           ALNC S+FI DLADAVIEALPSATALSPH SSTDADD DP +YAIKLLFGSVLA ILLLS
Sbjct: 421 ALNCNSTFISDLADAVIEALPSATALSPHTSSTDADDRDPLRYAIKLLFGSVLAFILLLS 480

Query: 481 PKAFLAFRNNFI 491
           PKAF+AFRNNF+
Sbjct: 481 PKAFMAFRNNFL 490

BLAST of Csor.00g166880 vs. TAIR 10
Match: AT5G26030.1 (ferrochelatase 1 )

HSP 1 Score: 598.2 bits (1541), Expect = 6.0e-171
Identity = 297/394 (75.38%), Postives = 343/394 (87.06%), Query Frame = 0

Query: 99  TYGEYALESRSQ-AAEEKVGVLLLNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQE 158
           +Y E + ++RS   AE+K+GVLLLNLGGP+TL+DVQPFLYNLFADPDIIRLPR F+FLQ 
Sbjct: 72  SYDETSAKARSHVVAEDKIGVLLLNLGGPETLNDVQPFLYNLFADPDIIRLPRPFQFLQG 131

Query: 159 PLAKLISTFRAPKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNTPANVYVGMRYWYP 218
            +AK IS  RAPKSKEGYA+IGGGSPLRKITDEQA A+KM+L+ KN  ANVYVGMRYWYP
Sbjct: 132 TIAKFISVVRAPKSKEGYAAIGGGSPLRKITDEQADAIKMSLQAKNIAANVYVGMRYWYP 191

Query: 219 FTEEAIQQIKRDGITRLVVLPLYPQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWY 278
           FTEEA+QQIK+D ITRLVVLPLYPQYSISTTGSSIRVLQ +FR+D YL+ +PV++IKSWY
Sbjct: 192 FTEEAVQQIKKDKITRLVVLPLYPQYSISTTGSSIRVLQDLFRKDPYLAGVPVAIIKSWY 251

Query: 279 QREGYIKSMADLVQAELKNFINPQEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQ 338
           QR GY+ SMADL++ EL+ F +P+EVMIFFSAHGVPVSYVENAGDPY+ QMEECI LIM+
Sbjct: 252 QRRGYVNSMADLIEKELQTFSDPKEVMIFFSAHGVPVSYVENAGDPYQKQMEECIDLIME 311

Query: 339 ELKARGIGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLE 398
           ELKARG+ N+H LAYQSRVGPVQWLKPYTDEVLV+LG+ G+KSLLAVPVSFVSEHIETLE
Sbjct: 312 ELKARGVLNDHKLAYQSRVGPVQWLKPYTDEVLVDLGKSGVKSLLAVPVSFVSEHIETLE 371

Query: 399 EIDMEYKHLALESGIENWGRVPALNCKSSFILDLADAVIEALPSATALS-PHA--SSTDA 458
           EIDMEY+ LALESG+ENWGRVPAL    SFI DLADAVIE+LPSA A+S P+A   S D+
Sbjct: 372 EIDMEYRELALESGVENWGRVPALGLTPSFITDLADAVIESLPSAEAMSNPNAVVDSEDS 431

Query: 459 DDCDPFQYAIKLLFGSVLAIILLLSPKAFLAFRN 489
           +  D F Y +K+ FGS+LA +LLLSPK F AFRN
Sbjct: 432 ESSDAFSYIVKMFFGSILAFVLLLSPKMFHAFRN 465

BLAST of Csor.00g166880 vs. TAIR 10
Match: AT5G26030.2 (ferrochelatase 1 )

HSP 1 Score: 598.2 bits (1541), Expect = 6.0e-171
Identity = 297/394 (75.38%), Postives = 343/394 (87.06%), Query Frame = 0

Query: 99  TYGEYALESRSQ-AAEEKVGVLLLNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQE 158
           +Y E + ++RS   AE+K+GVLLLNLGGP+TL+DVQPFLYNLFADPDIIRLPR F+FLQ 
Sbjct: 72  SYDETSAKARSHVVAEDKIGVLLLNLGGPETLNDVQPFLYNLFADPDIIRLPRPFQFLQG 131

Query: 159 PLAKLISTFRAPKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNTPANVYVGMRYWYP 218
            +AK IS  RAPKSKEGYA+IGGGSPLRKITDEQA A+KM+L+ KN  ANVYVGMRYWYP
Sbjct: 132 TIAKFISVVRAPKSKEGYAAIGGGSPLRKITDEQADAIKMSLQAKNIAANVYVGMRYWYP 191

Query: 219 FTEEAIQQIKRDGITRLVVLPLYPQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWY 278
           FTEEA+QQIK+D ITRLVVLPLYPQYSISTTGSSIRVLQ +FR+D YL+ +PV++IKSWY
Sbjct: 192 FTEEAVQQIKKDKITRLVVLPLYPQYSISTTGSSIRVLQDLFRKDPYLAGVPVAIIKSWY 251

Query: 279 QREGYIKSMADLVQAELKNFINPQEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQ 338
           QR GY+ SMADL++ EL+ F +P+EVMIFFSAHGVPVSYVENAGDPY+ QMEECI LIM+
Sbjct: 252 QRRGYVNSMADLIEKELQTFSDPKEVMIFFSAHGVPVSYVENAGDPYQKQMEECIDLIME 311

Query: 339 ELKARGIGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLE 398
           ELKARG+ N+H LAYQSRVGPVQWLKPYTDEVLV+LG+ G+KSLLAVPVSFVSEHIETLE
Sbjct: 312 ELKARGVLNDHKLAYQSRVGPVQWLKPYTDEVLVDLGKSGVKSLLAVPVSFVSEHIETLE 371

Query: 399 EIDMEYKHLALESGIENWGRVPALNCKSSFILDLADAVIEALPSATALS-PHA--SSTDA 458
           EIDMEY+ LALESG+ENWGRVPAL    SFI DLADAVIE+LPSA A+S P+A   S D+
Sbjct: 372 EIDMEYRELALESGVENWGRVPALGLTPSFITDLADAVIESLPSAEAMSNPNAVVDSEDS 431

Query: 459 DDCDPFQYAIKLLFGSVLAIILLLSPKAFLAFRN 489
           +  D F Y +K+ FGS+LA +LLLSPK F AFRN
Sbjct: 432 ESSDAFSYIVKMFFGSILAFVLLLSPKMFHAFRN 465

BLAST of Csor.00g166880 vs. TAIR 10
Match: AT2G30390.1 (ferrochelatase 2 )

HSP 1 Score: 506.9 bits (1304), Expect = 1.8e-143
Identity = 245/344 (71.22%), Postives = 297/344 (86.34%), Query Frame = 0

Query: 104 ALESRSQAAEE-KVGVLLLNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKL 163
           A+ S S   ++ K+GVLLLNLGGP+TLDDVQPFL+NLFADPDIIRLP +F+FLQ+PLA+ 
Sbjct: 88  AISSSSVITDDAKIGVLLLNLGGPETLDDVQPFLFNLFADPDIIRLPPVFQFLQKPLAQF 147

Query: 164 ISTFRAPKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNTPANVYVGMRYWYPFTEEA 223
           IS  RAPKSKEGYASIGGGSPLR ITD QA+ L+  L EKN PA VYVGMRYW+PFTEEA
Sbjct: 148 ISVARAPKSKEGYASIGGGSPLRHITDAQAEELRKCLWEKNVPAKVYVGMRYWHPFTEEA 207

Query: 224 IQQIKRDGITRLVVLPLYPQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWYQREGY 283
           I+QIKRDGIT+LVVLPLYPQ+SIST+GSS+R+L++IFRED YL  +  +VI SWYQREGY
Sbjct: 208 IEQIKRDGITKLVVLPLYPQFSISTSGSSLRLLERIFREDEYLVNMQHTVIPSWYQREGY 267

Query: 284 IKSMADLVQAELKNFINPQEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKAR 343
           IK+MA+L+Q+EL  F +P +V+IFFSAHGVP++YVE AGDPYK +MEEC+ LIM+EL  R
Sbjct: 268 IKAMANLIQSELGKFGSPNQVVIFFSAHGVPLAYVEEAGDPYKAEMEECVDLIMEELDKR 327

Query: 344 GIGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDME 403
            I N +TLAYQSRVGPV+WLKPYT+E + ELG+KG+++LLAVP+SFVSEHIETLEEID+E
Sbjct: 328 KITNAYTLAYQSRVGPVEWLKPYTEEAITELGKKGVENLLAVPISFVSEHIETLEEIDVE 387

Query: 404 YKHLALESGIENWGRVPALNCKSSFILDLADAVIEALPSATALS 447
           YK LAL+SGI+NWGRVPAL  +  FI DLADAV+E+LP   A++
Sbjct: 388 YKELALKSGIKNWGRVPALGTEPMFISDLADAVVESLPYVGAMA 431

BLAST of Csor.00g166880 vs. TAIR 10
Match: AT2G30390.2 (ferrochelatase 2 )

HSP 1 Score: 498.8 bits (1283), Expect = 4.9e-141
Identity = 245/354 (69.21%), Postives = 297/354 (83.90%), Query Frame = 0

Query: 104 ALESRSQAAEE-KVGVLLLNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKL 163
           A+ S S   ++ K+GVLLLNLGGP+TLDDVQPFL+NLFADPDIIRLP +F+FLQ+PLA+ 
Sbjct: 88  AISSSSVITDDAKIGVLLLNLGGPETLDDVQPFLFNLFADPDIIRLPPVFQFLQKPLAQF 147

Query: 164 ISTFRAPKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNTPANVYVGMRYWYPFTEEA 223
           IS  RAPKSKEGYASIGGGSPLR ITD QA+ L+  L EKN PA VYVGMRYW+PFTEEA
Sbjct: 148 ISVARAPKSKEGYASIGGGSPLRHITDAQAEELRKCLWEKNVPAKVYVGMRYWHPFTEEA 207

Query: 224 IQQIKRDGITRLVVLPLYPQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWYQREGY 283
           I+QIKRDGIT+LVVLPLYPQ+SIST+GSS+R+L++IFRED YL  +  +VI SWYQREGY
Sbjct: 208 IEQIKRDGITKLVVLPLYPQFSISTSGSSLRLLERIFREDEYLVNMQHTVIPSWYQREGY 267

Query: 284 IKSMADLVQAELKNFINPQ----------EVMIFFSAHGVPVSYVENAGDPYKDQMEECI 343
           IK+MA+L+Q+EL  F +P           +V+IFFSAHGVP++YVE AGDPYK +MEEC+
Sbjct: 268 IKAMANLIQSELGKFGSPNQTCFKPKYMLQVVIFFSAHGVPLAYVEEAGDPYKAEMEECV 327

Query: 344 YLIMQELKARGIGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEH 403
            LIM+EL  R I N +TLAYQSRVGPV+WLKPYT+E + ELG+KG+++LLAVP+SFVSEH
Sbjct: 328 DLIMEELDKRKITNAYTLAYQSRVGPVEWLKPYTEEAITELGKKGVENLLAVPISFVSEH 387

Query: 404 IETLEEIDMEYKHLALESGIENWGRVPALNCKSSFILDLADAVIEALPSATALS 447
           IETLEEID+EYK LAL+SGI+NWGRVPAL  +  FI DLADAV+E+LP   A++
Sbjct: 388 IETLEEIDVEYKELALKSGIKNWGRVPALGTEPMFISDLADAVVESLPYVGAMA 441

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P420443.0e-24488.01Ferrochelatase-2, chloroplastic OS=Cucumis sativus OX=3659 GN=HEMH PE=2 SV=1[more]
Q69TB13.9e-17574.04Ferrochelatase-1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os09... [more]
P420452.6e-17176.41Ferrochelatase-2, chloroplastic OS=Hordeum vulgare OX=4513 GN=HEMH PE=2 SV=1[more]
P420438.4e-17075.38Ferrochelatase-1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN... [more]
Q0DIV01.1e-14875.98Ferrochelatase-2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05... [more]
Match NameE-valueIdentityDescription
KAG6598300.10.0100.00Ferrochelatase-2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia]... [more]
XP_022962468.10.099.39ferrochelatase-2, chloroplastic isoform X1 [Cucurbita moschata][more]
XP_023546547.10.099.39ferrochelatase-2, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022997506.10.098.78ferrochelatase-2, chloroplastic isoform X1 [Cucurbita maxima][more]
XP_022962470.10.099.14ferrochelatase-2, chloroplastic isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1HEW30.099.39Ferrochelatase OS=Cucurbita moschata OX=3662 GN=LOC111462897 PE=3 SV=1[more]
A0A6J1K7N70.098.78Ferrochelatase OS=Cucurbita maxima OX=3661 GN=LOC111492405 PE=3 SV=1[more]
A0A6J1HF730.099.14Ferrochelatase OS=Cucurbita moschata OX=3662 GN=LOC111462897 PE=3 SV=1[more]
A0A6J1K5850.098.49Ferrochelatase OS=Cucurbita maxima OX=3661 GN=LOC111492405 PE=3 SV=1[more]
A0A5A7V5D01.23e-30988.21Ferrochelatase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00126... [more]
Match NameE-valueIdentityDescription
AT5G26030.16.0e-17175.38ferrochelatase 1 [more]
AT5G26030.26.0e-17175.38ferrochelatase 1 [more]
AT2G30390.11.8e-14371.22ferrochelatase 2 [more]
AT2G30390.24.9e-14169.21ferrochelatase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001015FerrochelataseTIGRFAMTIGR00109TIGR00109coord: 113..438
e-value: 4.5E-115
score: 382.4
IPR001015FerrochelatasePFAMPF00762Ferrochelatasecoord: 115..438
e-value: 1.7E-111
score: 372.3
IPR001015FerrochelatasePANTHERPTHR11108FERROCHELATASEcoord: 50..490
IPR001015FerrochelataseHAMAPMF_00323Ferrochelatasecoord: 114..439
score: 37.142395
NoneNo IPR availableGENE3D3.40.50.1400coord: 115..439
e-value: 3.5E-122
score: 409.7
NoneNo IPR availableGENE3D3.40.50.1400coord: 277..420
e-value: 3.5E-122
score: 409.7
NoneNo IPR availablePANTHERPTHR11108:SF4FERROCHELATASE-1, CHLOROPLASTIC/MITOCHONDRIALcoord: 50..490
NoneNo IPR availableSUPERFAMILY53800Chelatasecoord: 114..446
IPR019772Ferrochelatase, active sitePROSITEPS00534FERROCHELATASEcoord: 305..324
IPR033659Ferrochelatase, N-terminalCDDcd03411Ferrochelatase_Ncoord: 116..278
e-value: 1.44757E-71
score: 222.445
IPR033644Ferrochelatase, C-terminalCDDcd00419Ferrochelatase_Ccoord: 286..422
e-value: 2.59164E-57
score: 184.655

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g166880.m01Csor.00g166880.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006783 heme biosynthetic process
cellular_component GO:0009507 chloroplast
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005739 mitochondrion
molecular_function GO:0004325 ferrochelatase activity