Csor.00g164840 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g164840
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionRegulator of Vps4 activity in the MVB pathway protein
LocationCsor_Chr04: 7861204 .. 7863079 (-)
RNA-Seq ExpressionCsor.00g164840
SyntenyCsor.00g164840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAAAGAAGCTTGATGCACTGCTGGGAAGAAGCTTCAAGACCACGAGGTTCAAATCTCTAGTTAATCTGGCCATCTCTCGGCTCGCTGTCCTTGCAAACCAGCGCCAGGTGAGATCCTCCCAGGCTCGTTCTGACGTAGTTCAGTTGCTGCAGCTTGGCCACCAAGAGAGAGCCCTCCTTCGTGTAAGATCATTTTTTAAGTACTTTTAAACATCGGTTTCGGCTCCATAACCTTCAAAGCTTATAATAGGAGTCTTTGACAGGTTGAGCAAGTGATTAAGGACCAGAAACTGCTGGATGCATATGTGATTATAGAGAGTTACTGTGAGGCTGTACTTGAAAGAATCAGGCTACTTGAACAACAGAGGTCGGTAAATGAACCTGTAAATGTGAACATAAAGAGATGGATTTGGTCTGATTCTGATAATTTTGTTTTTGATTATGTAGGGAATGTCCAGAAGAACTGAAGGAGGCAATTTCAGGTTTGATTTTTGCATCTTCAAGATGTGGGGATTTCCCTGAGCTAATAGAGATTCGTTCAGCCATTACCAGTCGTTTTGGCAAGGAGTTTACTGCTCGTGCTATGGAGTTGCGCAACAATTGTGCTGTTGGTCCAATGGTATGCTTTGCTCTATGTTCTCGTTATTTGGCCTAAATCCACGGCTAACAAATATTATAAAGGATGTTTCGTTCTCCTCCCCAATCGATATACGATCTCACAATCAACCTCCTTTGGTGCCCAGCGTCCTCGCTGGCACTAGTTTCTTATCCTATCGATGTAGGACTCTCCAATCCACCTCCTTCGGGCCCAGCATCTTTGCTGGCACACTGCCTCGTGTCCACTCCTTTTGAGCCCCAATGTCCTTACTGGCACACCACCTCATGTCCATCACCTTCGGGGCTCAGCCTCCTCACTGGCACATGGCCCGATGTCTGGCTATGATACCGTTTGTAGCGGTCCAAGCCACCGCTAGCAGATACTGTTTCTTTGGGTTTTCTTTTTCGGGTTTTCCCTCAAAGTTTTTAAAATGCGTTTGCTAGAAAAAGGTTTCATACCCTTGTAAAGAATGTTTCATTCTCCTCTTCTACCGATAATGAGATCTTCTCAACTTTTAAGCAGTGTTTGCTCAAGTTTATATGCCAATTTTCCCTTGCTGTTTTCACTGAGTGATGTGGTAATCTTGGGGTTTTCAGATAGTAAAGAAGCTGTCAGCGAGGCCACCAAGCTTAGAGGTCAAATTGGAGTTACTTAAACAAGTTGCTTCTGAAAATGGCAGTACTTTGAAAGATTTGGAGGCCTATGAAGTTTCAACTGAGGTAGGAAATGAAATTAATGATCCATTACAAGTTCTTGCGTTATTGAGTGCCTAAAAAATAGATGCAGGAATCCAAAGTGGAAGGCGTTTATGAGATTTTACCTGAAGAGATAGAGAAAGAGAGTTGGTATGCTGATTCGATGAGAGGAAGAAAGAAGTACAAAGACGTGGGAGACGCTGCCCAGGCAGCATTTGAGTCGGCTGCTTGTGCAGCAGCTGCAGCCAGAGCTGCTGTGGAACTCGCTCAATCTAAATCACCTGATCAAGATGATGTGAGTAATCCGAGCCGAAAACCAAGAACATTCACTGAAATTCATGAATCCAAGAACAGTGAGATAGAATTAGAATCAAATAACAAGGAATTGAAGCAGCCAGTGTGTTCCTCTAGTTTAAAGGAGAATGAGAATCCAATTGAAGCAGTGAATATAACCGATCTGCTGGAGAAGGATTTGGTTTTTGAGGAGAGTGATGTTGATGGTGGGAGTGGAGAAAGCAGCAGTTCAACTCTTGAGGAGAAAGTCGAGGTTAATAGGACAATAGATGTCCGTAGGCACTGA

mRNA sequence

ATGGGAAAGAAGCTTGATGCACTGCTGGGAAGAAGCTTCAAGACCACGAGGTTCAAATCTCTAGTTAATCTGGCCATCTCTCGGCTCGCTGTCCTTGCAAACCAGCGCCAGGTGAGATCCTCCCAGGCTCGTTCTGACGTAGTTCAGTTGCTGCAGCTTGGCCACCAAGAGAGAGCCCTCCTTCGTGTTGAGCAAGTGATTAAGGACCAGAAACTGCTGGATGCATATGTGATTATAGAGAGTTACTGTGAGGCTGTACTTGAAAGAATCAGGCTACTTGAACAACAGAGGGAATGTCCAGAAGAACTGAAGGAGGCAATTTCAGGTTTGATTTTTGCATCTTCAAGATGTGGGGATTTCCCTGAGCTAATAGAGATTCGTTCAGCCATTACCAGTCGTTTTGGCAAGGAGTTTACTGCTCGTGCTATGGAGTTGCGCAACAATTGTGCTGTTGGTCCAATGATAGTAAAGAAGCTGTCAGCGAGGCCACCAAGCTTAGAGGTCAAATTGGAGTTACTTAAACAAGTTGCTTCTGAAAATGGCAGTACTTTGAAAGATTTGGAGGCCTATGAAGTTTCAACTGAGGAATCCAAAGTGGAAGGCGTTTATGAGATTTTACCTGAAGAGATAGAGAAAGAGAGTTGGTATGCTGATTCGATGAGAGGAAGAAAGAAGTACAAAGACGTGGGAGACGCTGCCCAGGCAGCATTTGAGTCGGCTGCTTGTGCAGCAGCTGCAGCCAGAGCTGCTGTGGAACTCGCTCAATCTAAATCACCTGATCAAGATGATGTGAGTAATCCGAGCCGAAAACCAAGAACATTCACTGAAATTCATGAATCCAAGAACAGTGAGATAGAATTAGAATCAAATAACAAGGAATTGAAGCAGCCAGTGTGTTCCTCTAGTTTAAAGGAGAATGAGAATCCAATTGAAGCAGTGAATATAACCGATCTGCTGGAGAAGGATTTGGTTTTTGAGGAGAGTGATGTTGATGGTGGGAGTGGAGAAAGCAGCAGTTCAACTCTTGAGGAGAAAGTCGAGGTTAATAGGACAATAGATGTCCGTAGGCACTGA

Coding sequence (CDS)

ATGGGAAAGAAGCTTGATGCACTGCTGGGAAGAAGCTTCAAGACCACGAGGTTCAAATCTCTAGTTAATCTGGCCATCTCTCGGCTCGCTGTCCTTGCAAACCAGCGCCAGGTGAGATCCTCCCAGGCTCGTTCTGACGTAGTTCAGTTGCTGCAGCTTGGCCACCAAGAGAGAGCCCTCCTTCGTGTTGAGCAAGTGATTAAGGACCAGAAACTGCTGGATGCATATGTGATTATAGAGAGTTACTGTGAGGCTGTACTTGAAAGAATCAGGCTACTTGAACAACAGAGGGAATGTCCAGAAGAACTGAAGGAGGCAATTTCAGGTTTGATTTTTGCATCTTCAAGATGTGGGGATTTCCCTGAGCTAATAGAGATTCGTTCAGCCATTACCAGTCGTTTTGGCAAGGAGTTTACTGCTCGTGCTATGGAGTTGCGCAACAATTGTGCTGTTGGTCCAATGATAGTAAAGAAGCTGTCAGCGAGGCCACCAAGCTTAGAGGTCAAATTGGAGTTACTTAAACAAGTTGCTTCTGAAAATGGCAGTACTTTGAAAGATTTGGAGGCCTATGAAGTTTCAACTGAGGAATCCAAAGTGGAAGGCGTTTATGAGATTTTACCTGAAGAGATAGAGAAAGAGAGTTGGTATGCTGATTCGATGAGAGGAAGAAAGAAGTACAAAGACGTGGGAGACGCTGCCCAGGCAGCATTTGAGTCGGCTGCTTGTGCAGCAGCTGCAGCCAGAGCTGCTGTGGAACTCGCTCAATCTAAATCACCTGATCAAGATGATGTGAGTAATCCGAGCCGAAAACCAAGAACATTCACTGAAATTCATGAATCCAAGAACAGTGAGATAGAATTAGAATCAAATAACAAGGAATTGAAGCAGCCAGTGTGTTCCTCTAGTTTAAAGGAGAATGAGAATCCAATTGAAGCAGTGAATATAACCGATCTGCTGGAGAAGGATTTGGTTTTTGAGGAGAGTGATGTTGATGGTGGGAGTGGAGAAAGCAGCAGTTCAACTCTTGAGGAGAAAGTCGAGGTTAATAGGACAATAGATGTCCGTAGGCACTGA

Protein sequence

MGKKLDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERALLRVEQVIKDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRCGDFPELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSLEVKLELLKQVASENGSTLKDLEAYEVSTEESKVEGVYEILPEEIEKESWYADSMRGRKKYKDVGDAAQAAFESAACAAAAARAAVELAQSKSPDQDDVSNPSRKPRTFTEIHESKNSEIELESNNKELKQPVCSSSLKENENPIEAVNITDLLEKDLVFEESDVDGGSGESSSSTLEEKVEVNRTIDVRRH
Homology
BLAST of Csor.00g164840 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 4.6e-16
Identity = 56/174 (32.18%), Postives = 98/174 (56.32%), Query Frame = 0

Query: 10  GRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERALLRVEQVIKD 69
           G S+ + + K  + LA+SR+ +L N++       + +V +LL+  ++E A +RVE +I+D
Sbjct: 5   GPSYDSYKLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRD 64

Query: 70  QKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRCGDFPELIEIRSA 129
           + L++ + IIE  CE +  RI L+    E P E+KE+I  L+++S R    PEL +I++ 
Sbjct: 65  EYLIECFQIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPELEQIKNQ 124

Query: 130 ITSRFGKEFTARAMELRNNC----AVGPMIVKKLSARPPSLEVKLELLKQVASE 180
           + +++GK      +E   NC     V P IV KLS   P   +  + L ++A +
Sbjct: 125 LKAKYGK-----GLENEANCHCSTHVNPKIVHKLSYATPDPSIIFQTLSEIAEK 172

BLAST of Csor.00g164840 vs. ExPASy Swiss-Prot
Match: P53990 (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 6.2e-13
Identity = 51/175 (29.14%), Postives = 93/175 (53.14%), Query Frame = 0

Query: 8   LLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERALLRVEQVI 67
           +LG  FK  R +  + L I+RL +L  ++   + +AR ++   L  G  ERA +RVE +I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 68  KDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRC-GDFPELIEI 127
           ++  L++A  I+E YC+ +L R  L++  +E    L E++S LI+A+ R   +  EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 128 RSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSAR-PPSLEVKLELLKQVASEN 181
              + +++ KE+  +         V   ++ KLS   PP + V+  L++   + N
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYN 174

BLAST of Csor.00g164840 vs. ExPASy Swiss-Prot
Match: Q9CX00 (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 6.2e-13
Identity = 51/175 (29.14%), Postives = 93/175 (53.14%), Query Frame = 0

Query: 8   LLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERALLRVEQVI 67
           +LG  FK  R +  + L I+RL +L  ++   + +AR ++   L  G  ERA +RVE +I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 68  KDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRC-GDFPELIEI 127
           ++  L++A  I+E YC+ +L R  L++  +E    L E++S LI+A+ R   +  EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 128 RSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSAR-PPSLEVKLELLKQVASEN 181
              + +++ KE+  +         V   ++ KLS   PP + V+  L++   + N
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYN 174

BLAST of Csor.00g164840 vs. ExPASy Swiss-Prot
Match: Q568Z6 (IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 6.2e-13
Identity = 51/175 (29.14%), Postives = 93/175 (53.14%), Query Frame = 0

Query: 8   LLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERALLRVEQVI 67
           +LG  FK  R +  + L I+RL +L  ++   + +AR ++   L  G  ERA +RVE +I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 68  KDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRC-GDFPELIEI 127
           ++  L++A  I+E YC+ +L R  L++  +E    L E++S LI+A+ R   +  EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 128 RSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSAR-PPSLEVKLELLKQVASEN 181
              + +++ KE+  +         V   ++ KLS   PP + V+  L++   + N
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYN 174

BLAST of Csor.00g164840 vs. ExPASy Swiss-Prot
Match: Q3ZBV1 (IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 3.1e-12
Identity = 50/175 (28.57%), Postives = 92/175 (52.57%), Query Frame = 0

Query: 8   LLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERALLRVEQVI 67
           +LG   K  R +  + L I+RL +L  ++   + +AR ++   L  G  ERA +RVE +I
Sbjct: 1   MLGSGIKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 68  KDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRC-GDFPELIEI 127
           ++  L++A  I+E YC+ +L R  L++  +E    L E++S LI+A+ R   +  EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 128 RSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSAR-PPSLEVKLELLKQVASEN 181
              + +++ KE+  +         V   ++ KLS   PP + V+  L++   + N
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYN 174

BLAST of Csor.00g164840 vs. NCBI nr
Match: XP_022956933.1 (uncharacterized protein LOC111458470 isoform X2 [Cucurbita moschata] >KAG6601314.1 IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 661 bits (1706), Expect = 1.05e-238
Identity = 357/357 (100.00%), Postives = 357/357 (100.00%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60
           MGKKLDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL
Sbjct: 1   MGKKLDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60

Query: 61  LRVEQVIKDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRCGDF 120
           LRVEQVIKDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRCGDF
Sbjct: 61  LRVEQVIKDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRCGDF 120

Query: 121 PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSLEVKLELLKQVASEN 180
           PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSLEVKLELLKQVASEN
Sbjct: 121 PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSLEVKLELLKQVASEN 180

Query: 181 GSTLKDLEAYEVSTEESKVEGVYEILPEEIEKESWYADSMRGRKKYKDVGDAAQAAFESA 240
           GSTLKDLEAYEVSTEESKVEGVYEILPEEIEKESWYADSMRGRKKYKDVGDAAQAAFESA
Sbjct: 181 GSTLKDLEAYEVSTEESKVEGVYEILPEEIEKESWYADSMRGRKKYKDVGDAAQAAFESA 240

Query: 241 ACAAAAARAAVELAQSKSPDQDDVSNPSRKPRTFTEIHESKNSEIELESNNKELKQPVCS 300
           ACAAAAARAAVELAQSKSPDQDDVSNPSRKPRTFTEIHESKNSEIELESNNKELKQPVCS
Sbjct: 241 ACAAAAARAAVELAQSKSPDQDDVSNPSRKPRTFTEIHESKNSEIELESNNKELKQPVCS 300

Query: 301 SSLKENENPIEAVNITDLLEKDLVFEESDVDGGSGESSSSTLEEKVEVNRTIDVRRH 357
           SSLKENENPIEAVNITDLLEKDLVFEESDVDGGSGESSSSTLEEKVEVNRTIDVRRH
Sbjct: 301 SSLKENENPIEAVNITDLLEKDLVFEESDVDGGSGESSSSTLEEKVEVNRTIDVRRH 357

BLAST of Csor.00g164840 vs. NCBI nr
Match: XP_022956932.1 (uncharacterized protein LOC111458470 isoform X1 [Cucurbita moschata])

HSP 1 Score: 656 bits (1693), Expect = 1.09e-236
Identity = 357/359 (99.44%), Postives = 357/359 (99.44%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60
           MGKKLDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL
Sbjct: 1   MGKKLDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60

Query: 61  LRVEQVIKDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRCGDF 120
           LRVEQVIKDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRCGDF
Sbjct: 61  LRVEQVIKDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRCGDF 120

Query: 121 PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSLEVKLELLKQVASEN 180
           PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSLEVKLELLKQVASEN
Sbjct: 121 PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSLEVKLELLKQVASEN 180

Query: 181 GSTLKDLEAYEVSTE--ESKVEGVYEILPEEIEKESWYADSMRGRKKYKDVGDAAQAAFE 240
           GSTLKDLEAYEVSTE  ESKVEGVYEILPEEIEKESWYADSMRGRKKYKDVGDAAQAAFE
Sbjct: 181 GSTLKDLEAYEVSTEMQESKVEGVYEILPEEIEKESWYADSMRGRKKYKDVGDAAQAAFE 240

Query: 241 SAACAAAAARAAVELAQSKSPDQDDVSNPSRKPRTFTEIHESKNSEIELESNNKELKQPV 300
           SAACAAAAARAAVELAQSKSPDQDDVSNPSRKPRTFTEIHESKNSEIELESNNKELKQPV
Sbjct: 241 SAACAAAAARAAVELAQSKSPDQDDVSNPSRKPRTFTEIHESKNSEIELESNNKELKQPV 300

Query: 301 CSSSLKENENPIEAVNITDLLEKDLVFEESDVDGGSGESSSSTLEEKVEVNRTIDVRRH 357
           CSSSLKENENPIEAVNITDLLEKDLVFEESDVDGGSGESSSSTLEEKVEVNRTIDVRRH
Sbjct: 301 CSSSLKENENPIEAVNITDLLEKDLVFEESDVDGGSGESSSSTLEEKVEVNRTIDVRRH 359

BLAST of Csor.00g164840 vs. NCBI nr
Match: XP_022956934.1 (uncharacterized protein LOC111458470 isoform X3 [Cucurbita moschata])

HSP 1 Score: 640 bits (1650), Expect = 3.34e-230
Identity = 349/351 (99.43%), Postives = 349/351 (99.43%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60
           MGKKLDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL
Sbjct: 1   MGKKLDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60

Query: 61  LRVEQVIKDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRCGDF 120
           LRVEQVIKDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRCGDF
Sbjct: 61  LRVEQVIKDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRCGDF 120

Query: 121 PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSLEVKLELLKQVASEN 180
           PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSLEVKLELLKQVASEN
Sbjct: 121 PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSLEVKLELLKQVASEN 180

Query: 181 GSTLKDLEAYEVSTE--ESKVEGVYEILPEEIEKESWYADSMRGRKKYKDVGDAAQAAFE 240
           GSTLKDLEAYEVSTE  ESKVEGVYEILPEEIEKESWYADSMRGRKKYKDVGDAAQAAFE
Sbjct: 181 GSTLKDLEAYEVSTEMQESKVEGVYEILPEEIEKESWYADSMRGRKKYKDVGDAAQAAFE 240

Query: 241 SAACAAAAARAAVELAQSKSPDQDDVSNPSRKPRTFTEIHESKNSEIELESNNKELKQPV 300
           SAACAAAAARAAVELAQSKSPDQDDVSNPSRKPRTFTEIHESKNSEIELESNNKELKQPV
Sbjct: 241 SAACAAAAARAAVELAQSKSPDQDDVSNPSRKPRTFTEIHESKNSEIELESNNKELKQPV 300

Query: 301 CSSSLKENENPIEAVNITDLLEKDLVFEESDVDGGSGESSSSTLEEKVEVN 349
           CSSSLKENENPIEAVNITDLLEKDLVFEESDVDGGSGESSSSTLEEKVEVN
Sbjct: 301 CSSSLKENENPIEAVNITDLLEKDLVFEESDVDGGSGESSSSTLEEKVEVN 351

BLAST of Csor.00g164840 vs. NCBI nr
Match: XP_023547113.1 (uncharacterized protein LOC111806012 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 609 bits (1571), Expect = 3.63e-218
Identity = 337/358 (94.13%), Postives = 341/358 (95.25%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60
           MGKKLDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL
Sbjct: 1   MGKKLDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60

Query: 61  LRVEQVIKDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRCGDF 120
            RVEQVIKDQKLLDAYVI+ESYCEAVLERI LLEQQRECPEELKEAISGLIFASSRCGDF
Sbjct: 61  FRVEQVIKDQKLLDAYVIMESYCEAVLERINLLEQQRECPEELKEAISGLIFASSRCGDF 120

Query: 121 PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSLEVKLELLKQVASEN 180
           PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPS E+KL+LLKQVASEN
Sbjct: 121 PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSSEIKLKLLKQVASEN 180

Query: 181 GSTLKDLEAYEVSTEESKVEGVYEILPEEIEKESWYADSMRGRKKYKDVGDAAQAAFESA 240
           G TLKDLEAYEVSTEESKVEGV   LPEEIEKESWYADSMRGRKKYKDVGDAAQAAFESA
Sbjct: 181 GITLKDLEAYEVSTEESKVEGV---LPEEIEKESWYADSMRGRKKYKDVGDAAQAAFESA 240

Query: 241 ACAAAAARAAVELAQSKSPDQDDVSNPSRKPRTFTEIHESKNSEIELESNNKELKQPVCS 300
           ACAAAAARAAVELAQSKS D D VSNPS K RTFTE HE KN+EIELESNNKELKQPVCS
Sbjct: 241 ACAAAAARAAVELAQSKSSDHDYVSNPSPKARTFTETHECKNNEIELESNNKELKQPVCS 300

Query: 301 SSLKENENPIEAVNITDLLEKDLVFEESDVDGGSGESSSS-TLEEKVEVNRTIDVRRH 357
           SSLKENE PIEAVNITDLLEKDLVFEESDVDGGSGESSSS TLEEK EVNRTIDVRRH
Sbjct: 301 SSLKENEIPIEAVNITDLLEKDLVFEESDVDGGSGESSSSSTLEEKFEVNRTIDVRRH 355

BLAST of Csor.00g164840 vs. NCBI nr
Match: XP_023547103.1 (uncharacterized protein LOC111806012 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 604 bits (1558), Expect = 3.73e-216
Identity = 337/360 (93.61%), Postives = 341/360 (94.72%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60
           MGKKLDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL
Sbjct: 1   MGKKLDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60

Query: 61  LRVEQVIKDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRCGDF 120
            RVEQVIKDQKLLDAYVI+ESYCEAVLERI LLEQQRECPEELKEAISGLIFASSRCGDF
Sbjct: 61  FRVEQVIKDQKLLDAYVIMESYCEAVLERINLLEQQRECPEELKEAISGLIFASSRCGDF 120

Query: 121 PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSLEVKLELLKQVASEN 180
           PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPS E+KL+LLKQVASEN
Sbjct: 121 PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSSEIKLKLLKQVASEN 180

Query: 181 GSTLKDLEAYEVSTE--ESKVEGVYEILPEEIEKESWYADSMRGRKKYKDVGDAAQAAFE 240
           G TLKDLEAYEVSTE  ESKVEGV   LPEEIEKESWYADSMRGRKKYKDVGDAAQAAFE
Sbjct: 181 GITLKDLEAYEVSTEMQESKVEGV---LPEEIEKESWYADSMRGRKKYKDVGDAAQAAFE 240

Query: 241 SAACAAAAARAAVELAQSKSPDQDDVSNPSRKPRTFTEIHESKNSEIELESNNKELKQPV 300
           SAACAAAAARAAVELAQSKS D D VSNPS K RTFTE HE KN+EIELESNNKELKQPV
Sbjct: 241 SAACAAAAARAAVELAQSKSSDHDYVSNPSPKARTFTETHECKNNEIELESNNKELKQPV 300

Query: 301 CSSSLKENENPIEAVNITDLLEKDLVFEESDVDGGSGESSSS-TLEEKVEVNRTIDVRRH 357
           CSSSLKENE PIEAVNITDLLEKDLVFEESDVDGGSGESSSS TLEEK EVNRTIDVRRH
Sbjct: 301 CSSSLKENEIPIEAVNITDLLEKDLVFEESDVDGGSGESSSSSTLEEKFEVNRTIDVRRH 357

BLAST of Csor.00g164840 vs. ExPASy TrEMBL
Match: A0A6J1GYN8 (uncharacterized protein LOC111458470 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111458470 PE=3 SV=1)

HSP 1 Score: 661 bits (1706), Expect = 5.10e-239
Identity = 357/357 (100.00%), Postives = 357/357 (100.00%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60
           MGKKLDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL
Sbjct: 1   MGKKLDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60

Query: 61  LRVEQVIKDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRCGDF 120
           LRVEQVIKDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRCGDF
Sbjct: 61  LRVEQVIKDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRCGDF 120

Query: 121 PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSLEVKLELLKQVASEN 180
           PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSLEVKLELLKQVASEN
Sbjct: 121 PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSLEVKLELLKQVASEN 180

Query: 181 GSTLKDLEAYEVSTEESKVEGVYEILPEEIEKESWYADSMRGRKKYKDVGDAAQAAFESA 240
           GSTLKDLEAYEVSTEESKVEGVYEILPEEIEKESWYADSMRGRKKYKDVGDAAQAAFESA
Sbjct: 181 GSTLKDLEAYEVSTEESKVEGVYEILPEEIEKESWYADSMRGRKKYKDVGDAAQAAFESA 240

Query: 241 ACAAAAARAAVELAQSKSPDQDDVSNPSRKPRTFTEIHESKNSEIELESNNKELKQPVCS 300
           ACAAAAARAAVELAQSKSPDQDDVSNPSRKPRTFTEIHESKNSEIELESNNKELKQPVCS
Sbjct: 241 ACAAAAARAAVELAQSKSPDQDDVSNPSRKPRTFTEIHESKNSEIELESNNKELKQPVCS 300

Query: 301 SSLKENENPIEAVNITDLLEKDLVFEESDVDGGSGESSSSTLEEKVEVNRTIDVRRH 357
           SSLKENENPIEAVNITDLLEKDLVFEESDVDGGSGESSSSTLEEKVEVNRTIDVRRH
Sbjct: 301 SSLKENENPIEAVNITDLLEKDLVFEESDVDGGSGESSSSTLEEKVEVNRTIDVRRH 357

BLAST of Csor.00g164840 vs. ExPASy TrEMBL
Match: A0A6J1GXR2 (uncharacterized protein LOC111458470 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458470 PE=3 SV=1)

HSP 1 Score: 656 bits (1693), Expect = 5.26e-237
Identity = 357/359 (99.44%), Postives = 357/359 (99.44%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60
           MGKKLDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL
Sbjct: 1   MGKKLDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60

Query: 61  LRVEQVIKDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRCGDF 120
           LRVEQVIKDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRCGDF
Sbjct: 61  LRVEQVIKDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRCGDF 120

Query: 121 PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSLEVKLELLKQVASEN 180
           PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSLEVKLELLKQVASEN
Sbjct: 121 PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSLEVKLELLKQVASEN 180

Query: 181 GSTLKDLEAYEVSTE--ESKVEGVYEILPEEIEKESWYADSMRGRKKYKDVGDAAQAAFE 240
           GSTLKDLEAYEVSTE  ESKVEGVYEILPEEIEKESWYADSMRGRKKYKDVGDAAQAAFE
Sbjct: 181 GSTLKDLEAYEVSTEMQESKVEGVYEILPEEIEKESWYADSMRGRKKYKDVGDAAQAAFE 240

Query: 241 SAACAAAAARAAVELAQSKSPDQDDVSNPSRKPRTFTEIHESKNSEIELESNNKELKQPV 300
           SAACAAAAARAAVELAQSKSPDQDDVSNPSRKPRTFTEIHESKNSEIELESNNKELKQPV
Sbjct: 241 SAACAAAAARAAVELAQSKSPDQDDVSNPSRKPRTFTEIHESKNSEIELESNNKELKQPV 300

Query: 301 CSSSLKENENPIEAVNITDLLEKDLVFEESDVDGGSGESSSSTLEEKVEVNRTIDVRRH 357
           CSSSLKENENPIEAVNITDLLEKDLVFEESDVDGGSGESSSSTLEEKVEVNRTIDVRRH
Sbjct: 301 CSSSLKENENPIEAVNITDLLEKDLVFEESDVDGGSGESSSSTLEEKVEVNRTIDVRRH 359

BLAST of Csor.00g164840 vs. ExPASy TrEMBL
Match: A0A6J1H0H7 (uncharacterized protein LOC111458470 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111458470 PE=3 SV=1)

HSP 1 Score: 640 bits (1650), Expect = 1.62e-230
Identity = 349/351 (99.43%), Postives = 349/351 (99.43%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60
           MGKKLDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL
Sbjct: 1   MGKKLDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60

Query: 61  LRVEQVIKDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRCGDF 120
           LRVEQVIKDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRCGDF
Sbjct: 61  LRVEQVIKDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRCGDF 120

Query: 121 PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSLEVKLELLKQVASEN 180
           PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSLEVKLELLKQVASEN
Sbjct: 121 PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSLEVKLELLKQVASEN 180

Query: 181 GSTLKDLEAYEVSTE--ESKVEGVYEILPEEIEKESWYADSMRGRKKYKDVGDAAQAAFE 240
           GSTLKDLEAYEVSTE  ESKVEGVYEILPEEIEKESWYADSMRGRKKYKDVGDAAQAAFE
Sbjct: 181 GSTLKDLEAYEVSTEMQESKVEGVYEILPEEIEKESWYADSMRGRKKYKDVGDAAQAAFE 240

Query: 241 SAACAAAAARAAVELAQSKSPDQDDVSNPSRKPRTFTEIHESKNSEIELESNNKELKQPV 300
           SAACAAAAARAAVELAQSKSPDQDDVSNPSRKPRTFTEIHESKNSEIELESNNKELKQPV
Sbjct: 241 SAACAAAAARAAVELAQSKSPDQDDVSNPSRKPRTFTEIHESKNSEIELESNNKELKQPV 300

Query: 301 CSSSLKENENPIEAVNITDLLEKDLVFEESDVDGGSGESSSSTLEEKVEVN 349
           CSSSLKENENPIEAVNITDLLEKDLVFEESDVDGGSGESSSSTLEEKVEVN
Sbjct: 301 CSSSLKENENPIEAVNITDLLEKDLVFEESDVDGGSGESSSSTLEEKVEVN 351

BLAST of Csor.00g164840 vs. ExPASy TrEMBL
Match: A0A6J1J1I7 (uncharacterized protein LOC111480936 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111480936 PE=3 SV=1)

HSP 1 Score: 603 bits (1556), Expect = 3.14e-216
Identity = 328/350 (93.71%), Postives = 336/350 (96.00%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60
           MGKKLDALLGRSF TTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL
Sbjct: 1   MGKKLDALLGRSFNTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60

Query: 61  LRVEQVIKDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRCGDF 120
            RVEQVIKDQKLLDAYVIIESYCEAVLERI+LLEQQRECPEELKEAISGLIFASSRCGDF
Sbjct: 61  FRVEQVIKDQKLLDAYVIIESYCEAVLERIKLLEQQRECPEELKEAISGLIFASSRCGDF 120

Query: 121 PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSLEVKLELLKQVASEN 180
           PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPS E+KL+LLKQVASEN
Sbjct: 121 PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSSEIKLKLLKQVASEN 180

Query: 181 GSTLKDLEAYEVSTEESKVEGVYEILPEEIEKESWYADSMRGRKKYKDVGDAAQAAFESA 240
           G TLKDLEAYEVSTEESKVEGV++ILPEE+EKESWYADSMRGRKKYKDVGDAAQAAFESA
Sbjct: 181 GITLKDLEAYEVSTEESKVEGVFKILPEEMEKESWYADSMRGRKKYKDVGDAAQAAFESA 240

Query: 241 ACAAAAARAAVELAQSKSPDQDDVSNPSRKPRTFTEIHESKNSEIELESNNKELKQPVCS 300
           ACAAAAARAAVEL QSKS D D VSNPS K RTFTE HESKN+EIELESNNKELKQPVCS
Sbjct: 241 ACAAAAARAAVELVQSKSSDHDYVSNPSPKARTFTETHESKNNEIELESNNKELKQPVCS 300

Query: 301 SSLKENENPIEAVNITDLLEKDLVFEESDVDGGSGESSSSTLEEKVEVNR 350
           SSLKENE PIEAVNI DLLEKDLVFEESDVDGGSGESSSSTLEEK EVN+
Sbjct: 301 SSLKENEIPIEAVNIIDLLEKDLVFEESDVDGGSGESSSSTLEEKFEVNK 350

BLAST of Csor.00g164840 vs. ExPASy TrEMBL
Match: A0A6J1IVF5 (uncharacterized protein LOC111480936 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111480936 PE=3 SV=1)

HSP 1 Score: 598 bits (1543), Expect = 3.23e-214
Identity = 328/352 (93.18%), Postives = 336/352 (95.45%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60
           MGKKLDALLGRSF TTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL
Sbjct: 1   MGKKLDALLGRSFNTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60

Query: 61  LRVEQVIKDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRCGDF 120
            RVEQVIKDQKLLDAYVIIESYCEAVLERI+LLEQQRECPEELKEAISGLIFASSRCGDF
Sbjct: 61  FRVEQVIKDQKLLDAYVIIESYCEAVLERIKLLEQQRECPEELKEAISGLIFASSRCGDF 120

Query: 121 PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSLEVKLELLKQVASEN 180
           PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPS E+KL+LLKQVASEN
Sbjct: 121 PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSSEIKLKLLKQVASEN 180

Query: 181 GSTLKDLEAYEVSTE--ESKVEGVYEILPEEIEKESWYADSMRGRKKYKDVGDAAQAAFE 240
           G TLKDLEAYEVSTE  ESKVEGV++ILPEE+EKESWYADSMRGRKKYKDVGDAAQAAFE
Sbjct: 181 GITLKDLEAYEVSTEMQESKVEGVFKILPEEMEKESWYADSMRGRKKYKDVGDAAQAAFE 240

Query: 241 SAACAAAAARAAVELAQSKSPDQDDVSNPSRKPRTFTEIHESKNSEIELESNNKELKQPV 300
           SAACAAAAARAAVEL QSKS D D VSNPS K RTFTE HESKN+EIELESNNKELKQPV
Sbjct: 241 SAACAAAAARAAVELVQSKSSDHDYVSNPSPKARTFTETHESKNNEIELESNNKELKQPV 300

Query: 301 CSSSLKENENPIEAVNITDLLEKDLVFEESDVDGGSGESSSSTLEEKVEVNR 350
           CSSSLKENE PIEAVNI DLLEKDLVFEESDVDGGSGESSSSTLEEK EVN+
Sbjct: 301 CSSSLKENEIPIEAVNIIDLLEKDLVFEESDVDGGSGESSSSTLEEKFEVNK 352

BLAST of Csor.00g164840 vs. TAIR 10
Match: AT1G13340.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 255.8 bits (652), Expect = 5.3e-68
Identity = 169/377 (44.83%), Postives = 232/377 (61.54%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60
           MGKKLDALLGRSFKT +FKSL+ LA++RL++L NQRQ R SQA SDV +LL+LG  E A 
Sbjct: 1   MGKKLDALLGRSFKTNKFKSLITLALTRLSILKNQRQARLSQAISDVTELLKLGQHEHAY 60

Query: 61  LRVEQVIKDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRCGDF 120
            RV+QV+KDQ  LD    I  Y    L+RI L E  R+CPEEL EA+SGL+FA+SR G+F
Sbjct: 61  HRVDQVVKDQNTLDVLFFIHGYFTLCLDRIHLFEHNRDCPEELLEAVSGLLFAASRIGEF 120

Query: 121 PELIEIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSLEVKLELLKQVASEN 180
           PEL EIR+ + SRFGK+  AR++ELR+NC V P I++KLS RPP  EV+++ LK++A+EN
Sbjct: 121 PELQEIRNVLISRFGKDLAARSIELRSNCGVDPKIIQKLSTRPPPKEVRMKALKEIAAEN 180

Query: 181 GSTLKDLEAYEVSTE-ESKVEGVYEILPEEIEKES------WYADSM-RGRKKYKDVGDA 240
              LK L+    STE  + ++G  ++   ++  +         +DS+ RG+KKYKDV DA
Sbjct: 181 NIVLK-LDQASTSTEGTTNMQGTSDVSKTKLTSKDGRGEGYGLSDSVKRGKKKYKDVADA 240

Query: 241 AQAAFESAACAAAAARAAVELAQSKSPDQDDVSNPSRKPRTFTEIHESKNSEIELE---- 300
           AQAAFESAA AA AA+AAVEL+Q      D   N   +       H S+N E E E    
Sbjct: 241 AQAAFESAAHAAEAAQAAVELSQFSPRGHDSPGNIGGE----NSFHGSENKESEQEQEGN 300

Query: 301 SNNKELKQPVCSSSLKENENPIEAVNI---------TDLLEKDLVFEESDVDGGSGESSS 357
            ++ E +  V S S +   +  + +++           LLEKD +  +S+ +      + 
Sbjct: 301 DDSSEGEGDVMSESKRSMSDSEDIIDVPVMSFREDPVKLLEKDTIIYDSEEETQPSVETI 360

BLAST of Csor.00g164840 vs. TAIR 10
Match: AT1G34220.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 143.3 bits (360), Expect = 3.8e-34
Identity = 71/176 (40.34%), Postives = 116/176 (65.91%), Query Frame = 0

Query: 5   LDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERALLRVE 64
           LD+   + FK  + K+L+ L I R+ ++ N+R+ +  Q R ++ +LL+ G +  A +RVE
Sbjct: 4   LDSFFNKGFKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVE 63

Query: 65  QVIKDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRCGDFPELI 124
            +I+++K++ A  I+E +CE +  R+ ++E QRECP +LKEAIS + FA+ RC D  EL 
Sbjct: 64  HIIREEKMMAAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQ 123

Query: 125 EIRSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSLEVKLELLKQVASEN 181
           +++    S++GKEF A A EL+ +  V   +V+ LS R PS E KL+LLK++A E+
Sbjct: 124 QVQILFVSKYGKEFVAAASELKPDSGVNRKLVELLSVRAPSPETKLKLLKEIAEEH 179

BLAST of Csor.00g164840 vs. TAIR 10
Match: AT2G19710.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 134.0 bits (336), Expect = 2.3e-31
Identity = 67/173 (38.73%), Postives = 115/173 (66.47%), Query Frame = 0

Query: 8   LLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERALLRVEQVI 67
           +L R FK  + K+ + +A SRL +L N+++++  Q R ++ QLL+ G    A +RVE V+
Sbjct: 4   VLQRGFKPAKCKTALQMANSRLKILKNKKEIQIKQLRRELAQLLESGQTPTARIRVEHVV 63

Query: 68  KDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRCGDFPELIEIR 127
           +++K + AY +I  YCE ++ R+ ++E Q+ CP +LKEA++ ++FAS R  D PEL EI 
Sbjct: 64  REEKTVAAYELIGIYCELLVVRLGVIESQKNCPIDLKEAVTSVLFASQRLSDVPELSEIF 123

Query: 128 SAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSLEVKLELLKQVASEN 181
              T+++GK+F+  A+ELR +  V  ++V+KLSA+ P    K+++L  +A E+
Sbjct: 124 KQFTTKYGKDFSTSAVELRPDSGVSRLLVEKLSAKAPDGPTKVKILMAIAEEH 176

BLAST of Csor.00g164840 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 134.0 bits (336), Expect = 2.3e-31
Identity = 90/278 (32.37%), Postives = 147/278 (52.88%), Query Frame = 0

Query: 7   ALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERALLRVEQV 66
           +L  R F +++ K+   +A++R+ ++ N+R V   Q R D+  LLQ G    A +RVE V
Sbjct: 20  SLFRRGFNSSKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHV 79

Query: 67  IKDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRCGDFPELIEI 126
           I++Q +  A  IIE +CE ++ R+ ++ +Q++CP +LKE I+ LIFA+ RC + PEL ++
Sbjct: 80  IREQNIQAANEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDL 139

Query: 127 RSAITSRFGKEFTARAMELRNNCAVGPMIVKKLSARPPSLEVKLELLKQVASENGSTLKD 186
           R     ++GK+F + A +LR +C V  M++ KLS R P  E KL+++K++A E       
Sbjct: 140 RDIFAKKYGKDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAKEFQVDWDT 199

Query: 187 LEAYE--VSTEESKVEGVYEIL-----------------PEEIEKESWYADSMRGRKKYK 246
            E  +  +  +E  ++G  + +                 P +    S    SM     Y 
Sbjct: 200 TETEQELLKPQEESIDGPRKFVSASSLPVNRAAINEPIDPTKAVPRS--TSSMSINTHYH 259

Query: 247 DVGDAAQAAFESAACAAAAARAAVELAQSKSPDQDDVS 266
           D   AA+AA E A  A AAA+ A  LA  +     + S
Sbjct: 260 DTESAAEAATELAKQAVAAAQVASLLATRRDSSNKEFS 295

BLAST of Csor.00g164840 vs. TAIR 10
Match: AT1G34220.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 127.9 bits (320), Expect = 1.7e-29
Identity = 71/206 (34.47%), Postives = 116/206 (56.31%), Query Frame = 0

Query: 5   LDALLGRSFKTTRFKSLVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERALLRVE 64
           LD+   + FK  + K+L+ L I R+ ++ N+R+ +  Q R ++ +LL+ G +  A +RVE
Sbjct: 4   LDSFFNKGFKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVE 63

Query: 65  QVIKDQKLLDAYVIIESYCEAVLERIRLLEQQRECPEELKEAISGLIFASSRCGDFPELI 124
            +I+++K++ A  I+E +CE +  R+ ++E QRECP +LKEAIS + FA+ RC D  EL 
Sbjct: 64  HIIREEKMMAAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQ 123

Query: 125 EIRSAITSRFGKEFTARAMELRNNCAVGP------------------------------M 181
           +++    S++GKEF A A EL+ +  V                                 
Sbjct: 124 QVQILFVSKYGKEFVAAASELKPDSGVNRKTESLIFIAWFSLVETRDLFMFLYFSNSILQ 183

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q54I394.6e-1632.18IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1[more]
P539906.2e-1329.14IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1[more]
Q9CX006.2e-1329.14IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1[more]
Q568Z66.2e-1329.14IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1[more]
Q3ZBV13.1e-1228.57IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022956933.11.05e-238100.00uncharacterized protein LOC111458470 isoform X2 [Cucurbita moschata] >KAG6601314... [more]
XP_022956932.11.09e-23699.44uncharacterized protein LOC111458470 isoform X1 [Cucurbita moschata][more]
XP_022956934.13.34e-23099.43uncharacterized protein LOC111458470 isoform X3 [Cucurbita moschata][more]
XP_023547113.13.63e-21894.13uncharacterized protein LOC111806012 isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023547103.13.73e-21693.61uncharacterized protein LOC111806012 isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1GYN85.10e-239100.00uncharacterized protein LOC111458470 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1GXR25.26e-23799.44uncharacterized protein LOC111458470 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1H0H71.62e-23099.43uncharacterized protein LOC111458470 isoform X3 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1J1I73.14e-21693.71uncharacterized protein LOC111480936 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1IVF53.23e-21493.18uncharacterized protein LOC111480936 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
AT1G13340.15.3e-6844.83Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G34220.23.8e-3440.34Regulator of Vps4 activity in the MVB pathway protein [more]
AT2G19710.12.3e-3138.73Regulator of Vps4 activity in the MVB pathway protein [more]
AT4G35730.12.3e-3132.37Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G34220.11.7e-2934.47Regulator of Vps4 activity in the MVB pathway protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005061Vacuolar protein sorting-associated protein Ist1PFAMPF03398Ist1coord: 19..181
e-value: 8.2E-53
score: 178.7
IPR005061Vacuolar protein sorting-associated protein Ist1PANTHERPTHR12161IST1 FAMILY MEMBERcoord: 8..341
IPR042277Vacuolar protein sorting-associated protein IST1-likeGENE3D1.20.1260.60coord: 6..191
e-value: 5.1E-63
score: 214.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 263..291
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 326..357
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 254..293
NoneNo IPR availablePANTHERPTHR12161:SF16REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEINcoord: 8..341

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g164840.m01Csor.00g164840.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015031 protein transport