Csor.00g163510 (gene) Silver-seed gourd (wild; sororia) v1

BLAST of Csor.00g163510 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 859.4 bits (2219), Expect = 3.2e-248
Identity = 437/773 (56.53%), Postives = 547/773 (70.76%), Query Frame = 0

Query: 2   NLTNFILILFWFFPLISPVVAVKKSYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLG 61
           +L++ +L++  FF   SP  A+KKSYIV LGSH+H  ++S+  +  V  SH   L SF+G
Sbjct: 20  SLSSLLLLVTLFF---SPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVG 79

Query: 62  SFEKAKDAIFYSYKNHINGFAAILDEEEATNLAKHPEVATVLPNKARELHTTHSWEFMHL 121
           S E AK+AIFYSYK HINGFAAILDE EA  +AKHP+V +V PNK R+LHTTHSW FM L
Sbjct: 80  SHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLL 139

Query: 122 EKNGFIFPFSPWRKARFGKDVIIANLDTGVWPESKSFGEHGITGGVPSRWKGGCTDKTPD 181
            KNG +   S W KA +G+D IIANLDTGVWPESKSF + G  G VP+RWKG C      
Sbjct: 140 AKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGY-GAVPARWKGRCHKD--- 199

Query: 182 AVPCNKKLIGAKYFNQGVIAYL-KSHNLTDELPLIGNSTRDYVGHGTHTLSTAGGSYVSG 241
            VPCN+KLIGA+YFN+G +AY     N + E      + RD+ GHG+HTLSTA G++V G
Sbjct: 200 -VPCNRKLIGARYFNKGYLAYTGLPSNASYE------TCRDHDGHGSHTLSTAAGNFVPG 259

Query: 242 VSVFGSGIGTAKGGSPKARVAAYKVCWPFRNGGGCYDADIFDGFDQAIYDGVDVLSLSIG 301
            +VFG G GTA GGSPKARVAAYKVCWP  +G  C+DADI    + AI DGVDVLS S+G
Sbjct: 260 ANVFGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVG 319

Query: 302 SPPDEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQA 361
               +Y  D IAI SFHA+K G+ VVCSAGNSGP   T +N+APW++TVGAS++DREFQA
Sbjct: 320 GDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQA 379

Query: 362 PIELGNGQRFWGLSLSRPLARRKLYPLITGAQAKATTASADDAMLCKPKTLDHSKVNGKI 421
            +EL NGQ F G SLS+PL   K+Y LI+ A A     +  DA+LCK  +LD  KV GKI
Sbjct: 380 FVELKNGQSFKGTSLSKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKI 439

Query: 422 LVCLTGGSSRIDKGMQAVVAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSY 481
           LVCL G ++R+DKGMQA  AGA GM+LCND+ SG +II+D HVLPAS I Y DG+ + SY
Sbjct: 440 LVCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSY 499

Query: 482 INSRKNPMGYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDVTAPGVDIIAAFSGA 541
           ++S K+P GY+  P++ +NTKP+P MA+FSSRGPN ++P I+KPD+TAPGV+IIAAF+ A
Sbjct: 500 LSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEA 559

Query: 542 VSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDN 601
             PT    DNR  P+ T SGTSMSCPH+SG+VGLLK LHP WSPAAI+SAIMT++   +N
Sbjct: 560 TGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNN 619

Query: 602 TRNLILDGGSPIFAPATPFIYGSGHIHPIGAIDPGLVYDLSPNDYLEFLCASGYKAKNFR 661
            R  ++D     F  A PF YGSGH+ P  A  PGLVYDL+  DYL+FLCA GY     +
Sbjct: 620 RRKPMVDES---FKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQ 679

Query: 662 VFGE-KNFKCPISGSILNFNYPSIGVQNLTGCVTLTRRLKNVGRPGVYRVRVRRPKGVKV 721
           +F E   + C    ++L+FNYPSI V NLTG +T+TR+LKNVG P  Y  R R P GV+V
Sbjct: 680 LFAEDPQYTCRQGANLLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFREPLGVRV 739

Query: 722 LVKPRVLKFRKIGEEKRFELTM--IGAVAEGQIGYGTLIWTDGKHFVKSPIVV 771
            V+P+ L F K GE K F++T+  +     G + +G L WTD  H+V+SPIVV
Sbjct: 740 SVEPKQLTFNKTGEVKIFQMTLRPLPVTPSGYV-FGELTWTDSHHYVRSPIVV 774

BLAST of Csor.00g163510 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 1.6e-228
Identity = 410/774 (52.97%), Postives = 537/774 (69.38%), Query Frame = 0

Query: 5   NFILILFWFFPLISPVVAVK--KSYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGS 64
           +F+L+L         ++A K   SY+V  G+HSH  E++   + RV ++H+  LGSF GS
Sbjct: 8   SFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGS 67

Query: 65  FEKAKDAIFYSYKNHINGFAAILDEEEATNLAKHPEVATVLPNKARELHTTHSWEFMHLE 124
            E+A DAIFYSY  HINGFAA LD + A  ++KHPEV +V PNKA +LHTT SW+F+ LE
Sbjct: 68  RERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLE 127

Query: 125 KNGFIFPFSPWRKARFGKDVIIANLDTGVWPESKSFGEHGITGGVPSRWKGGCTDKTPDA 184
            N ++   S WRKARFG+D IIANLDTGVWPESKSF + G+ G +PSRWKG C ++    
Sbjct: 128 HNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGL-GPIPSRWKGICQNQKDAT 187

Query: 185 VPCNKKLIGAKYFNQGVIAYLKSHNLTDELPLIGNSTRDYVGHGTHTLSTAGGSYVSGVS 244
             CN+KLIGA+YFN+G  A +   N + + P      RD  GHG+HTLSTA G +V GVS
Sbjct: 188 FHCNRKLIGARYFNKGYAAAVGHLNSSFDSP------RDLDGHGSHTLSTAAGDFVPGVS 247

Query: 245 VFGSGIGTAKGGSPKARVAAYKVCWPFRNGGGCYDADIFDGFDQAIYDGVDVLSLSIGSP 304
           +FG G GTAKGGSP+ARVAAYKVCWP   G  CYDAD+   FD AI+DG DV+S+S+G  
Sbjct: 248 IFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGE 307

Query: 305 PDEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQAPI 364
           P  +++D++AI SFHA KK I VVCSAGNSGP+ +T +N+APW +TVGAST+DREF + +
Sbjct: 308 PTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNL 367

Query: 365 ELGNGQRFWGLSL-SRPLARRKLYPLITGAQAKATTASADDAMLCKPKTLDHSKVNGKIL 424
            LGNG+ + G SL S  L   K YP++    AKA  ASA DA LCK  +LD  K  GKIL
Sbjct: 368 VLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKIL 427

Query: 425 VCLTGGSSRIDKGMQAVVAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYI 484
           VCL G + R++KG    + G +GM+L N   +G  ++AD HVLPA+ ++  D  AVS YI
Sbjct: 428 VCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYI 487

Query: 485 NSRKNPMGYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDVTAPGVDIIAAFSGAV 544
           +  K P+ ++ P  + +  KP+P MA+FSS+GP+IV+P+I+KPD+TAPGV +IAA++GAV
Sbjct: 488 SQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAV 547

Query: 545 SPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNT 604
           SPT E FD R + +  +SGTSMSCPH+SGI GLLK  +P WSPAAI+SAIMT+ATI D+ 
Sbjct: 548 SPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDI 607

Query: 605 RNLILDGGSPIFAPATPFIYGSGHIHPIGAIDPGLVYDLSPNDYLEFLCASGYKAKNFRV 664
              I +  +     ATPF +G+GH+ P  A++PGLVYDL   DYL FLC+ GY A    V
Sbjct: 608 PGPIQNATN---MKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISV 667

Query: 665 FGEKNFKCPISG-SILNFNYPSIGVQNLTGC-VTLTRRLKNVGRPGVYRVRVRRPKGVKV 724
           F   NF C     S++N NYPSI V NLT   VT++R +KNVGRP +Y V+V  P+GV V
Sbjct: 668 FSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYV 727

Query: 725 LVKPRVLKFRKIGEEKRFELTMI---GAVAEGQIGYGTLIWTDGKHFVKSPIVV 771
            VKP  L F K+GE+K F++ ++   G VA+G + +G L+W+D KH V+SPIVV
Sbjct: 728 AVKPTSLNFTKVGEQKTFKVILVKSKGNVAKGYV-FGELVWSDKKHRVRSPIVV 770

BLAST of Csor.00g163510 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 650.2 bits (1676), Expect = 2.9e-185
Identity = 374/792 (47.22%), Postives = 488/792 (61.62%), Query Frame = 0

Query: 3   LTNFILILFWFFPLISPVVAVKKSYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGS 62
           L++F L  F    L++ V   KK YIV +G+HSHG   ++ D++   DSH+ LLGS  GS
Sbjct: 12  LSSFFLFTF----LLAAVNGSKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFGS 71

Query: 63  FEKAKDAIFYSYKNHINGFAAILDEEEATNLAKHPEVATVLPNKARELHTTHSWEFMHLE 122
            EKAK+AI YSY  HINGFAA+L+EEEA ++AK+P V +V  +K  +LHTT SWEF+ L 
Sbjct: 72  REKAKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLH 131

Query: 123 KNGFIFPFSPWRKARFGKDVIIANLDTGVWPESKSFGEHGITGGVPSRWKGGC--TDKTP 182
           + G     S W+K RFG++ II N+DTGVWPES+SF + G  G VPS+W+GG    +K P
Sbjct: 132 RRG---QNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKGY-GTVPSKWRGGLCQINKLP 191

Query: 183 DAV--PCNKKLIGAKYFNQGVIAYLKSHNLTDELPLIGNSTRDYVGHGTHTLSTAGGSYV 242
            ++   CN+KLIGA+Y+N+      ++HN   +L  + ++ RD+VGHGTHTLSTAGG++V
Sbjct: 192 GSMKNTCNRKLIGARYYNKA----FEAHN--GQLDPLLHTARDFVGHGTHTLSTAGGNFV 251

Query: 243 SGVSVFGSGIGTAKGGSPKARVAAYKVCWPFRNGGGCYDADIFDGFDQAIYDGVDVLSLS 302
            G  VF  G GTAKGGSP+ARVAAYKVCW   +   CY AD+    DQAI DGVDV+++S
Sbjct: 252 PGARVFAVGNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVS 311

Query: 303 IG----SPPDEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTL 362
            G       +  + D I+I +FHA+ K I +V SAGN GP+  T  N+APW+ T+ ASTL
Sbjct: 312 FGVSYVVTAEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTL 371

Query: 363 DREFQAPIELGNGQRFWGLSLSRPLARRKLYPLITGAQAKATTASADDAMLCKPKTLDHS 422
           DR+F + + + N Q   G SL   L   + + LI    AK   A+  DA LC+  TLD +
Sbjct: 372 DRDFSSNLTI-NNQLIEGASLFVNLPPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRT 431

Query: 423 KVNGKILVCLTGGSSR-IDKGMQAVVAGAVGMILCNDRFSGFKIIADLHVL-----PASH 482
           KVNGKI++C   G  + + +G++A+ AGA GMIL N   +G  + A+ HV      P   
Sbjct: 432 KVNGKIVLCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRR 491

Query: 483 ISYNDGQAVSSYINSRKNPM--GYLIPPS---SKVNTKPSPTMAAFSSRGPNIVSPEIIK 542
                    ++ I    +P+  G  I  S   +    KP+P MA+FSSRGPN + P I+K
Sbjct: 492 AKSRPHGVKTTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILK 551

Query: 543 PDVTAPGVDIIAAFSGAVSPTGEPFDNRT-VPYITMSGTSMSCPHVSGIVGLLKALHPEW 602
           PDVTAPGV+I+AA+S   S +    DNR    +  + GTSMSCPH SGI GLLK  HP W
Sbjct: 552 PDVTAPGVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSW 611

Query: 603 SPAAIKSAIMTSATISDNTRNLILDGGSPIFAPATPFIYGSGHIHPIGAIDPGLVYDLSP 662
           SPAAIKSAIMT+AT  DNT   I D      A A  F YGSGH+ P  AI+PGLVYDLS 
Sbjct: 612 SPAAIKSAIMTTATTLDNTNRPIQDAFDKTLADA--FAYGSGHVRPDLAIEPGLVYDLSL 671

Query: 663 NDYLEFLCASGYKAKNFRVFG-EKNFKCPISGSILNFNYPSIGVQNL-TGCVTLTRRLKN 722
            DYL FLCASGY  +        + F C  S S+ + NYPSI + NL    VT+ R + N
Sbjct: 672 TDYLNFLCASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRLKPVTIARTVTN 731

Query: 723 VGRPGVYRVRVRRPKGVKVLVKPRVLKFRKIGEEKRFELTM--IGAVAEGQIGYGTLIWT 771
           VG P  Y V  R P G  + V P  L F KIGE K F++ +    A    +  +G L WT
Sbjct: 732 VGPPSTYTVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWT 786

BLAST of Csor.00g163510 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 3.6e-159
Identity = 334/782 (42.71%), Postives = 465/782 (59.46%), Query Frame = 0

Query: 1   MNLTNFILILFWFFPLISPVVAVKKSYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFL 60
           ++ T F L+L   F  +S   + + +YIV          ++   +    D H     S L
Sbjct: 6   LSSTAFFLLLCLGFCHVSSSSSDQGTYIV---------HMAKSQMPSSFDLHSNWYDSSL 65

Query: 61  GSFEKAKDAIFYSYKNHINGFAAILDEEEATNLAKHPEVATVLPNKARELHTTHSWEFMH 120
            S   + + + Y+Y+N I+GF+  L +EEA +L   P V +VLP    ELHTT +  F+ 
Sbjct: 66  RSISDSAE-LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLG 125

Query: 121 L-EKNGFIFPFSPWRKARFGKDVIIANLDTGVWPESKSFGEHGITGGVPSRWKGGCTDKT 180
           L E    +FP     +A    DV++  LDTGVWPESKS+ + G  G +PS WKGGC   T
Sbjct: 126 LDEHTADLFP-----EAGSYSDVVVGVLDTGVWPESKSYSDEGF-GPIPSSWKGGCEAGT 185

Query: 181 P-DAVPCNKKLIGAKYFNQGVIAYLKSHNLTDELPLIGNSTRDYVGHGTHTLSTAGGSYV 240
              A  CN+KLIGA++F +G  + +   + + E      S RD  GHGTHT STA GS V
Sbjct: 186 NFTASLCNRKLIGARFFARGYESTMGPIDESKE----SRSPRDDDGHGTHTSSTAAGSVV 245

Query: 241 SGVSVFGSGIGTAKGGSPKARVAAYKVCWPFRNGGGCYDADIFDGFDQAIYDGVDVLSLS 300
            G S+ G   GTA+G +P+ARVA YKVCW     GGC+ +DI    D+AI D V+VLS+S
Sbjct: 246 EGASLLGYASGTARGMAPRARVAVYKVCWL----GGCFSSDILAAIDKAIADNVNVLSMS 305

Query: 301 IGSPPDEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDREF 360
           +G    +YY D +AI +F A+++GI V CSAGN+GPS ++ +N+APWI TVGA TLDR+F
Sbjct: 306 LGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDF 365

Query: 361 QAPIELGNGQRFWGLSLSRPLA-RRKLYPLITGAQAKATTASADDAMLCKPKTLDHSKVN 420
            A   LGNG+ F G+SL +  A   KL P I    A    ++A +  LC   TL   KV 
Sbjct: 366 PALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNA----SNATNGNLCMTGTLIPEKVK 425

Query: 421 GKILVCLTGGSSRIDKGMQAVVAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAV 480
           GKI++C  G ++R+ KG     AG VGMIL N   +G +++AD H+LPA+ +    G  +
Sbjct: 426 GKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDII 485

Query: 481 SSYINSRKNPMGYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDVTAPGVDIIAAF 540
             Y+ +  NP   +    + V  KPSP +AAFSSRGPN ++P I+KPD+ APGV+I+AA+
Sbjct: 486 RHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAW 545

Query: 541 SGAVSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSA-- 600
           +GA  PTG   D+R V +  +SGTSMSCPHVSG+  LLK++HPEWSPAAI+SA+MT+A  
Sbjct: 546 TGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYK 605

Query: 601 TISDNTRNLILDGGSPIFAPATPFIYGSGHIHPIGAIDPGLVYDLSPNDYLEFLCASGYK 660
           T  D    L +  G     P+TPF +G+GH+ P  A +PGL+YDL+  DYL FLCA  Y 
Sbjct: 606 TYKDGKPLLDIATGK----PSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYT 665

Query: 661 AKNFRVFGEKNFKCPISG--SILNFNYPSIGVQ-NLTGCVTLTRRLKNVGRPGVYRVRV- 720
           +   R    +N+ C  S   S+ + NYPS  V  +  G    TR + +VG  G Y V+V 
Sbjct: 666 SPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVT 725

Query: 721 RRPKGVKVLVKPRVLKFRKIGEEKRFELTMI--GAVAEGQIGYGTLIWTDGKHFVKSPIV 772
               GVK+ V+P VL F++  E+K + +T     +   G   +G++ W+DGKH V SP+ 
Sbjct: 726 SETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVA 755

BLAST of Csor.00g163510 vs. ExPASy Swiss-Prot
Match: A9JQS7 (Subtilisin-like serine-protease S OS=Lotus japonicus OX=34305 GN=SbtS PE=2 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 7.5e-157
Identity = 332/777 (42.73%), Postives = 466/777 (59.97%), Query Frame = 0

Query: 3   LTNFILILFWFFPLISPVVAVKKSYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGS 62
           + +F L+LF  + L+    +  K YIV +G  SH       + + VV ++H++L S  GS
Sbjct: 6   ILSFTLLLFVGYTLVHG--STPKHYIVYMGDRSHP------NSESVVRANHEILASVTGS 65

Query: 63  FEKAKDAIFYSYKNHINGFAAILDEEEATNLAKHPEVATVLPNKARELHTTHSWEFMHLE 122
              AK A  + Y     GF+A++  E+A  LA H  V +V  +K  +LHTTHSW+F+ L+
Sbjct: 66  LNDAKAAAIHHYSRSFQGFSAMITPEQAKKLADHNSVVSVFESKMNKLHTTHSWDFLGLD 125

Query: 123 KNGFIFPFSPWRKARFGKDVIIANLDTGVWPESKSFGEHGITGGVPSRWKGGC-TDKTPD 182
               ++  +P        +VI+  +D+GVWPES+SF ++G+ G VP ++KG C T     
Sbjct: 126 T---VYKNNP-SALDSASNVIVGVIDSGVWPESESFNDYGL-GPVPEKFKGECVTGDNFT 185

Query: 183 AVPCNKKLIGAKYFNQGVIAYL-KSHNLTDELPLIGNSTRDYVGHGTHTLSTAGGSYVSG 242
              CNKK+IGA+++++G+ A +    N+ D   +   S RD  GHGTHT ST  GS VS 
Sbjct: 186 LANCNKKIIGARFYSKGLEAEIGPLENIVDS--IFFRSPRDSDGHGTHTASTIAGSIVSN 245

Query: 243 VSVFGSGIGTAKGGSPKARVAAYKVCWPFRNGGGCYDADIFDGFDQAIYDGVDVLSLSIG 302
           VS+FG   GTA+GG+P AR++ YK CW     G C DAD+F   D AI+DGVD+LSLS+G
Sbjct: 246 VSLFGMAKGTARGGAPSARLSIYKACW----FGFCSDADVFAAMDDAIHDGVDILSLSLG 305

Query: 303 SPPDE--YYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDREF 362
             P +  Y+++ I++ +FHA +KGI V  SAGNS     TA N+APWI TV AST+DREF
Sbjct: 306 PDPPQPLYFENAISVGAFHAFQKGILVSASAGNS-VFPRTACNVAPWIFTVAASTVDREF 365

Query: 363 QAPIELGNGQRFWGLSLSRPLARRKLYPLITGAQAKATTASADDAMLCKPKTLDHSKVNG 422
           ++ I LGN +   GLSL+ P+     Y LI G+ A A   +A +A  CK  TLD + + G
Sbjct: 366 RSDIYLGNSKVLKGLSLN-PIKMEGSYGLIYGSAAAAAGDAALNASFCKEHTLDPTLIKG 425

Query: 423 KILVCLTG--GSSRIDKGMQAVVAGAVGMILC--NDRFSGFKIIADLHVLPASHISYNDG 482
           KI++C       +R +K +     G VGMIL   N R  GF+      V+P++ I  +  
Sbjct: 426 KIVICTVEKFTDNRREKAIIIKQGGGVGMILIDHNARDVGFQF-----VIPSTMIGQDAV 485

Query: 483 QAVSSYINSRKNPMGYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDVTAPGVDII 542
           + + +Y+ + KNP   + P  + V TKP+P  AAFSS GPNI++P+IIKPD+T PGV+I+
Sbjct: 486 EELQAYMKTEKNPTATIFPTLTLVGTKPAPESAAFSSVGPNIITPDIIKPDITGPGVNIL 545

Query: 543 AAFSGAVSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTS 602
           AA+S     T    + ++V Y  +SGTSMSCPH+S I  ++K+ HP WSPAAI SAIMTS
Sbjct: 546 AAWSPVA--TEATVEQKSVNYNIISGTSMSCPHISAISAIIKSHHPSWSPAAIMSAIMTS 605

Query: 603 ATISDNTRNLILDGGSPIFAPATPFIYGSGHIHPIGAIDPGLVYDLSPNDYLEFLCASGY 662
           AT+ DNT +LI  G  P    ATPF YGSGH++P+ +++PGLVYD S  D L FLC++G 
Sbjct: 606 ATVMDNTHSLI--GRDPNGTQATPFDYGSGHVNPVASLNPGLVYDFSSQDVLNFLCSNGA 665

Query: 663 KAKNFRVFGEKNFKCPISGSI-LNFNYPSIGVQNLTGCVTLTRRLKNVGR-PGVYRVRVR 722
                +    +  +C  S +   NFNYPSIGV NL G +++ R +   G+ P  Y   V 
Sbjct: 666 SPAQLKNLTGELTQCQKSPTASYNFNYPSIGVSNLNGSLSVYRTVTYYGQEPTEYFASVE 725

Query: 723 RPKGVKVLVKPRVLKFRKIGEEKRFELTMIG-AVAEGQIGYGTLIWTDGKHFVKSPI 769
           RP GV V V P  LKF K GE+  F +       + G   +G L W +GK  V+SPI
Sbjct: 726 RPSGVIVRVTPAKLKFWKAGEKITFRIDFTPFKNSNGNFVFGALTWNNGKQRVRSPI 752

BLAST of Csor.00g163510 vs. NCBI nr
Match: KAG6583696.1 (Subtilisin-like protease 5.4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1564 bits (4050), Expect = 0.0
Identity = 775/775 (100.00%), Postives = 775/775 (100.00%), Query Frame = 0

Query: 1   MNLTNFILILFWFFPLISPVVAVKKSYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFL 60
           MNLTNFILILFWFFPLISPVVAVKKSYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFL
Sbjct: 1   MNLTNFILILFWFFPLISPVVAVKKSYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFL 60

Query: 61  GSFEKAKDAIFYSYKNHINGFAAILDEEEATNLAKHPEVATVLPNKARELHTTHSWEFMH 120
           GSFEKAKDAIFYSYKNHINGFAAILDEEEATNLAKHPEVATVLPNKARELHTTHSWEFMH
Sbjct: 61  GSFEKAKDAIFYSYKNHINGFAAILDEEEATNLAKHPEVATVLPNKARELHTTHSWEFMH 120

Query: 121 LEKNGFIFPFSPWRKARFGKDVIIANLDTGVWPESKSFGEHGITGGVPSRWKGGCTDKTP 180
           LEKNGFIFPFSPWRKARFGKDVIIANLDTGVWPESKSFGEHGITGGVPSRWKGGCTDKTP
Sbjct: 121 LEKNGFIFPFSPWRKARFGKDVIIANLDTGVWPESKSFGEHGITGGVPSRWKGGCTDKTP 180

Query: 181 DAVPCNKKLIGAKYFNQGVIAYLKSHNLTDELPLIGNSTRDYVGHGTHTLSTAGGSYVSG 240
           DAVPCNKKLIGAKYFNQGVIAYLKSHNLTDELPLIGNSTRDYVGHGTHTLSTAGGSYVSG
Sbjct: 181 DAVPCNKKLIGAKYFNQGVIAYLKSHNLTDELPLIGNSTRDYVGHGTHTLSTAGGSYVSG 240

Query: 241 VSVFGSGIGTAKGGSPKARVAAYKVCWPFRNGGGCYDADIFDGFDQAIYDGVDVLSLSIG 300
           VSVFGSGIGTAKGGSPKARVAAYKVCWPFRNGGGCYDADIFDGFDQAIYDGVDVLSLSIG
Sbjct: 241 VSVFGSGIGTAKGGSPKARVAAYKVCWPFRNGGGCYDADIFDGFDQAIYDGVDVLSLSIG 300

Query: 301 SPPDEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQA 360
           SPPDEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQA
Sbjct: 301 SPPDEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQA 360

Query: 361 PIELGNGQRFWGLSLSRPLARRKLYPLITGAQAKATTASADDAMLCKPKTLDHSKVNGKI 420
           PIELGNGQRFWGLSLSRPLARRKLYPLITGAQAKATTASADDAMLCKPKTLDHSKVNGKI
Sbjct: 361 PIELGNGQRFWGLSLSRPLARRKLYPLITGAQAKATTASADDAMLCKPKTLDHSKVNGKI 420

Query: 421 LVCLTGGSSRIDKGMQAVVAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSY 480
           LVCLTGGSSRIDKGMQAVVAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSY
Sbjct: 421 LVCLTGGSSRIDKGMQAVVAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSY 480

Query: 481 INSRKNPMGYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDVTAPGVDIIAAFSGA 540
           INSRKNPMGYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDVTAPGVDIIAAFSGA
Sbjct: 481 INSRKNPMGYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDVTAPGVDIIAAFSGA 540

Query: 541 VSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDN 600
           VSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDN
Sbjct: 541 VSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDN 600

Query: 601 TRNLILDGGSPIFAPATPFIYGSGHIHPIGAIDPGLVYDLSPNDYLEFLCASGYKAKNFR 660
           TRNLILDGGSPIFAPATPFIYGSGHIHPIGAIDPGLVYDLSPNDYLEFLCASGYKAKNFR
Sbjct: 601 TRNLILDGGSPIFAPATPFIYGSGHIHPIGAIDPGLVYDLSPNDYLEFLCASGYKAKNFR 660

Query: 661 VFGEKNFKCPISGSILNFNYPSIGVQNLTGCVTLTRRLKNVGRPGVYRVRVRRPKGVKVL 720
           VFGEKNFKCPISGSILNFNYPSIGVQNLTGCVTLTRRLKNVGRPGVYRVRVRRPKGVKVL
Sbjct: 661 VFGEKNFKCPISGSILNFNYPSIGVQNLTGCVTLTRRLKNVGRPGVYRVRVRRPKGVKVL 720

Query: 721 VKPRVLKFRKIGEEKRFELTMIGAVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 775
           VKPRVLKFRKIGEEKRFELTMIGAVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF
Sbjct: 721 VKPRVLKFRKIGEEKRFELTMIGAVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 775

BLAST of Csor.00g163510 vs. NCBI nr
Match: XP_022927103.1 (subtilisin-like protease SBT5.4 [Cucurbita moschata])

HSP 1 Score: 1481 bits (3834), Expect = 0.0
Identity = 729/753 (96.81%), Postives = 740/753 (98.27%), Query Frame = 0

Query: 23  VKKSYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGSFEKAKDAIFYSYKNHINGFA 82
           + +SYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGS EKAKDAIFYSYKNHINGFA
Sbjct: 16  ILQSYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFA 75

Query: 83  AILDEEEATNLAKHPEVATVLPNKARELHTTHSWEFMHLEKNGFIFPFSPWRKARFGKDV 142
           AILDEEEATNLAKHPEVA VLPNKA+ELHTTHSWEFMHLEKNGFIFPFSPWRKARFGKDV
Sbjct: 76  AILDEEEATNLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNGFIFPFSPWRKARFGKDV 135

Query: 143 IIANLDTGVWPESKSFGEHGITGGVPSRWKGGCTDKTPDAVPCNKKLIGAKYFNQGVIAY 202
           IIANLDTGVWPESKSFGE GITGGVPSRWKGGC DKTPDAVPCNKKLIGAKYFNQG+IAY
Sbjct: 136 IIANLDTGVWPESKSFGEQGITGGVPSRWKGGCMDKTPDAVPCNKKLIGAKYFNQGLIAY 195

Query: 203 LKSHNLTDELPLIGNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAA 262
           LKSHNLTDELPLI NSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAA
Sbjct: 196 LKSHNLTDELPLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAA 255

Query: 263 YKVCWPFRNGGGCYDADIFDGFDQAIYDGVDVLSLSIGSPPDEYYDDTIAIASFHALKKG 322
           YKVCWPFRNGGGCYDADIFDGFDQAI+DGVDVLSLSIGSPPDEYYDDTIAIASFHALKKG
Sbjct: 256 YKVCWPFRNGGGCYDADIFDGFDQAIHDGVDVLSLSIGSPPDEYYDDTIAIASFHALKKG 315

Query: 323 IPVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQAPIELGNGQRFWGLSLSRPLARR 382
           IPVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQAPIELGNGQRFWGLSLSRPLARR
Sbjct: 316 IPVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQAPIELGNGQRFWGLSLSRPLARR 375

Query: 383 KLYPLITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVVAGA 442
           KLYPLITGAQAKATT SADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAV+AGA
Sbjct: 376 KLYPLITGAQAKATTTSADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGA 435

Query: 443 VGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLIPPSSKVNTKP 502
           VGMILCNDRFSGFKIIADLHVLPASHISYNDGQA+SSYINSRKNPMGYLIPPSSKVNTKP
Sbjct: 436 VGMILCNDRFSGFKIIADLHVLPASHISYNDGQAISSYINSRKNPMGYLIPPSSKVNTKP 495

Query: 503 SPTMAAFSSRGPNIVSPEIIKPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTS 562
           SPTMAAFSSRGPNIVSPEIIKPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTS
Sbjct: 496 SPTMAAFSSRGPNIVSPEIIKPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTS 555

Query: 563 MSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTRNLILDGGSPIFAPATPFIYG 622
           MSCPHVSGIVGLLKALHP+WSPAAIKSAIMTSATISDNT NLILDGGSPIFAPATPFIYG
Sbjct: 556 MSCPHVSGIVGLLKALHPQWSPAAIKSAIMTSATISDNTMNLILDGGSPIFAPATPFIYG 615

Query: 623 SGHIHPIGAIDPGLVYDLSPNDYLEFLCASGYKAKNFRVFGEKNFKCPISGSILNFNYPS 682
           SGHIHPIGAIDPGLVYDLSPNDYLEFLCASGYK KN RVF E+N+KCP+SGSILNFNYPS
Sbjct: 616 SGHIHPIGAIDPGLVYDLSPNDYLEFLCASGYKEKNIRVFAEENYKCPVSGSILNFNYPS 675

Query: 683 IGVQNLTGCVTLTRRLKNVGRPGVYRVRVRRPKGVKVLVKPRVLKFRKIGEEKRFELTMI 742
           IGVQNLTGCVTLTRRLKNVGRPGVYRVRVRRPKGVKVLVKPRVLKFRKIGEEKRFELTM 
Sbjct: 676 IGVQNLTGCVTLTRRLKNVGRPGVYRVRVRRPKGVKVLVKPRVLKFRKIGEEKRFELTMT 735

Query: 743 GAVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 775
           G VAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF
Sbjct: 736 GVVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 768

BLAST of Csor.00g163510 vs. NCBI nr
Match: XP_022927102.1 (subtilisin-like protease SBT5.4 [Cucurbita moschata])

HSP 1 Score: 1400 bits (3625), Expect = 0.0
Identity = 691/752 (91.89%), Postives = 717/752 (95.35%), Query Frame = 0

Query: 24   KKSYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGSFEKAKDAIFYSYKNHINGFAA 83
            K+SYIVLLGSHSHGLEVSA+D+QRVVDSHHKLLGS LGS EKAKDAIF+SYKNHINGFAA
Sbjct: 251  KESYIVLLGSHSHGLEVSAVDLQRVVDSHHKLLGSLLGSSEKAKDAIFHSYKNHINGFAA 310

Query: 84   ILDEEEATNLAKHPEVATVLPNKARELHTTHSWEFMHLEKNGFIFPFSPWRKARFGKDVI 143
            IL+EEEAT LAKHPEVA VLPNKA+ELHTTHSWEFMHLEKNG + PFSPWRKARFG+DVI
Sbjct: 311  ILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNGVVLPFSPWRKARFGRDVI 370

Query: 144  IANLDTGVWPESKSFGEHGITGGVPSRWKGGCTDKTPDAVPCNKKLIGAKYFNQGVIAYL 203
            IANLDTGVWPESKSFGE GI GGVPSRWKGGC DKTPDAVPCN+KLIGAKYFNQG+IAYL
Sbjct: 371  IANLDTGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDAVPCNRKLIGAKYFNQGLIAYL 430

Query: 204  KSHNLTDELPLIGNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAY 263
            KSHNLTDEL LI NSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAY
Sbjct: 431  KSHNLTDELSLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAY 490

Query: 264  KVCWPFRNGGGCYDADIFDGFDQAIYDGVDVLSLSIGSPPDEYYDDTIAIASFHALKKGI 323
            KVCWPF N GGCYD D+ DGFDQAI+DGVDVLSLSIGSPP EYYDDTIAIASF A+KKGI
Sbjct: 491  KVCWPFHNSGGCYDIDVVDGFDQAIHDGVDVLSLSIGSPPAEYYDDTIAIASFLAIKKGI 550

Query: 324  PVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQAPIELGNGQRFWGLSLSRPLARRK 383
            PVVCSAGNSGPSMA+A+NIAPWILTVGASTLDREFQAPIELGNGQRFWG SLSRPLARRK
Sbjct: 551  PVVCSAGNSGPSMASASNIAPWILTVGASTLDREFQAPIELGNGQRFWGSSLSRPLARRK 610

Query: 384  LYPLITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVVAGAV 443
            LYPLITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAV+AGAV
Sbjct: 611  LYPLITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAV 670

Query: 444  GMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLIPPSSKVNTKPS 503
            GMILCNDRFSGFKIIADLHVLPASHI+YNDGQAV SYINS KNPMGYLIPPSSKVNTKPS
Sbjct: 671  GMILCNDRFSGFKIIADLHVLPASHINYNDGQAVFSYINSWKNPMGYLIPPSSKVNTKPS 730

Query: 504  PTMAAFSSRGPNIVSPEIIKPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSM 563
            PTMAAFSSRGPN+VSPEIIKPDVTAPGV+IIAAFSGAVSPT EPFDNRTVPYITMSGTSM
Sbjct: 731  PTMAAFSSRGPNMVSPEIIKPDVTAPGVNIIAAFSGAVSPTREPFDNRTVPYITMSGTSM 790

Query: 564  SCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTRNLILDGGSPIFAPATPFIYGS 623
            SCPHVSGIVGLLKALHPEWSPAAIKSAIMTSA ISDNT NLILDGGSP+FAPATPFIYGS
Sbjct: 791  SCPHVSGIVGLLKALHPEWSPAAIKSAIMTSAIISDNTMNLILDGGSPMFAPATPFIYGS 850

Query: 624  GHIHPIGAIDPGLVYDLSPNDYLEFLCASGYKAKNFRVFGEKNFKCPISGSILNFNYPSI 683
            GHIHP GAIDPGLVYDLSPNDYLEFLCASGYK KN RVF ++NFKCPIS SI NFNYPSI
Sbjct: 851  GHIHPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNIRVFADENFKCPISCSIFNFNYPSI 910

Query: 684  GVQNLTGCVTLTRRLKNVGRPGVYRVRVRRPKGVKVLVKPRVLKFRKIGEEKRFELTMIG 743
            GVQNLTG VTL RRLKNVGRPGVYRVRVR+P+GVKV VKPRVLKF KIGEEKRFELTM G
Sbjct: 911  GVQNLTGSVTLKRRLKNVGRPGVYRVRVRQPEGVKVSVKPRVLKFWKIGEEKRFELTMTG 970

Query: 744  AVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 775
            A+AEGQIGYGTLIWTDGKHFV+SPIVVSSG F
Sbjct: 971  AMAEGQIGYGTLIWTDGKHFVRSPIVVSSGLF 1002

BLAST of Csor.00g163510 vs. NCBI nr
Match: XP_022973086.1 (subtilisin-like protease SBT5.4 [Cucurbita maxima])

HSP 1 Score: 1400 bits (3623), Expect = 0.0
Identity = 703/775 (90.71%), Postives = 717/775 (92.52%), Query Frame = 0

Query: 1   MNLTNFILILFWFFPLISPVVAVKKSYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFL 60
           MN TNFILILFWFF LISPV+AVKKSYIVLLGSHSHGLEVS ID+QRVVDSHHKLLGSFL
Sbjct: 1   MNFTNFILILFWFFRLISPVIAVKKSYIVLLGSHSHGLEVSTIDLQRVVDSHHKLLGSFL 60

Query: 61  GSFEKAKDAIFYSYKNHINGFAAILDEEEATNLAKHPEVATVLPNKARELHTTHSWEFMH 120
           GS EKAKDAIFYSYKNHINGFAAILDEEEAT  AKHPEVA VLPNKA+ELHTTHSWEFMH
Sbjct: 61  GSSEKAKDAIFYSYKNHINGFAAILDEEEATKFAKHPEVAAVLPNKAKELHTTHSWEFMH 120

Query: 121 LEKNGFIFPFSPWRKARFGKDVIIANLDTGVWPESKSFGEHGITGGVPSRWKGGCTDKTP 180
           LEKNGFI PFSPWRKARFGKDVIIANLDTGVWPESKSFGE GI GGVPSRWKGGCTDKTP
Sbjct: 121 LEKNGFILPFSPWRKARFGKDVIIANLDTGVWPESKSFGEQGIVGGVPSRWKGGCTDKTP 180

Query: 181 DAVPCNKKLIGAKYFNQGVIAYLKSHNLTDELPLIGNSTRDYVGHGTHTLSTAGGSYVSG 240
           DAVPCN+KLIGAKYFNQGVIAYLKS NLTDELPLI NSTRDYVGHG+HTLSTAGG+YVSG
Sbjct: 181 DAVPCNRKLIGAKYFNQGVIAYLKSQNLTDELPLIVNSTRDYVGHGSHTLSTAGGNYVSG 240

Query: 241 VSVFGSGIGTAKGGSPKARVAAYKVCWPFRNGGGCYDADIFDGFDQAIYDGVDVLSLSIG 300
           VSVFGSGIGTAKGGSPKARVAAYKVCWPF N GGCYDADIFDG DQAIYDGVDVLSLSIG
Sbjct: 241 VSVFGSGIGTAKGGSPKARVAAYKVCWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLSIG 300

Query: 301 SPPDEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQA 360
           SPP EYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQA
Sbjct: 301 SPPAEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQA 360

Query: 361 PIELGNGQRFWGLSLSRPLARRKLYPLITGAQAKATTASADDAMLCKPKTLDHSKVNGKI 420
           PIELGNGQRFWG SLSRPLA RKLYPLITGAQAKATTASADDAMLCKPKTLD SKVNGKI
Sbjct: 361 PIELGNGQRFWGSSLSRPLAGRKLYPLITGAQAKATTASADDAMLCKPKTLDRSKVNGKI 420

Query: 421 LVCLTGGSSRIDKGMQAVVAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSY 480
           LVCLTGGSSRIDKGMQAV+AGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSY
Sbjct: 421 LVCLTGGSSRIDKGMQAVLAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSY 480

Query: 481 INSRKNPMGYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDVTAPGVDIIAAFSGA 540
           INSRKNPMGYLIPPSSKVNTKPSP MAAFSSRGPN++SPEIIKPDVTAPGV+IIAAFSGA
Sbjct: 481 INSRKNPMGYLIPPSSKVNTKPSPIMAAFSSRGPNMISPEIIKPDVTAPGVNIIAAFSGA 540

Query: 541 VSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDN 600
           VSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDN
Sbjct: 541 VSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDN 600

Query: 601 TRNLILDGGSPIFAPATPFIYGSGHIHPIGAIDPGLVYDLSPNDYLEFLCASGYKAKNFR 660
           T NL+LDGGSPIFAPATPFIYGSGHIHP GAIDPGL                        
Sbjct: 601 TMNLMLDGGSPIFAPATPFIYGSGHIHPTGAIDPGL------------------------ 660

Query: 661 VFGEKNFKCPISGSILNFNYPSIGVQNLTGCVTLTRRLKNVGRPGVYRVRVRRPKGVKVL 720
                   CP+SGSILNFNYPSIGVQNLTG VT+TRRLKNVGRPGVYRVRVRRP+GVKV 
Sbjct: 661 --------CPVSGSILNFNYPSIGVQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVS 720

Query: 721 VKPRVLKFRKIGEEKRFELTMIGAVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 775
           VKPRVLKF KIGEEKRFELTM GAVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF
Sbjct: 721 VKPRVLKFWKIGEEKRFELTMTGAVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 743

BLAST of Csor.00g163510 vs. NCBI nr
Match: KAG6583695.1 (Subtilisin-like protease 5.4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1357 bits (3513), Expect = 0.0
Identity = 685/743 (92.19%), Postives = 686/743 (92.33%), Query Frame = 0

Query: 33  SHSHGLEVSAIDVQRVVDSHHKLLGSFLGSFEKAKDAIFYSYKNHINGFAAILDEEEATN 92
           SHSHGLEVSAIDVQRVVDSHHKLLGSFLGSFEKAKDAIFYSYKNHINGFAAILDEEEATN
Sbjct: 5   SHSHGLEVSAIDVQRVVDSHHKLLGSFLGSFEKAKDAIFYSYKNHINGFAAILDEEEATN 64

Query: 93  LAKHPEVATVLPNKARELHTTHSWEFMHLEKNGFIFPFSPWRKARFGKDVIIANLDTGVW 152
           LA                                                        VW
Sbjct: 65  LAS-------------------------------------------------------VW 124

Query: 153 PESKSFGEHGITGGVPSRWKGGCTDKTPDAVPCNKKLIGAKYFNQGVIAYLKSHNLTDEL 212
           PESKSFGEHGITGGVPSRWKGGCTDKTPDAVPCNKKLIGAKYFNQGVIAYLKSHNLTDEL
Sbjct: 125 PESKSFGEHGITGGVPSRWKGGCTDKTPDAVPCNKKLIGAKYFNQGVIAYLKSHNLTDEL 184

Query: 213 PLIGNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKVCWPFRNG 272
           PLIGNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKVCWPFRNG
Sbjct: 185 PLIGNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKVCWPFRNG 244

Query: 273 GGCYDADIFDGFDQAIYDGVDVLSLSIGSPPDEYYDDTIAIASFHALKKGIPVVCSAGNS 332
           GGCYDADIFDGFDQAIYDGVDVLSLSIGSPPDEYYDDTIAIASFHALKKGIPVVCSAGNS
Sbjct: 245 GGCYDADIFDGFDQAIYDGVDVLSLSIGSPPDEYYDDTIAIASFHALKKGIPVVCSAGNS 304

Query: 333 GPSMATATNIAPWILTVGASTLDREFQAPIELGNGQRFWGLSLSRPLARRKLYPLITGAQ 392
           GPSMATATNIAPWILTVGASTLDREFQAPIELGNGQRFWGLSLSRPLARRKLYPLITGAQ
Sbjct: 305 GPSMATATNIAPWILTVGASTLDREFQAPIELGNGQRFWGLSLSRPLARRKLYPLITGAQ 364

Query: 393 AKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVVAGAVGMILCNDRF 452
           AKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVVAGAVGMILCNDRF
Sbjct: 365 AKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVVAGAVGMILCNDRF 424

Query: 453 SGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLIPPSSKVNTKPSPTMAAFSSR 512
           SGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLIPPSSKVNTKPSPTMAAFSSR
Sbjct: 425 SGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLIPPSSKVNTKPSPTMAAFSSR 484

Query: 513 GPNIVSPEIIKPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSCPHVSGIV 572
           GPNIVSPEIIKPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSCPHVSGIV
Sbjct: 485 GPNIVSPEIIKPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSCPHVSGIV 544

Query: 573 GLLKALHPEWSPAAIKSAIMTSATISDNTRNLILDGGSPIFAPATPFIYGSGHIHPIGAI 632
           GLLKALHPEWSPAAIKSAIMTSATISDNTRNLILDGGSPIFAPATPFIYGSGHIHPIGAI
Sbjct: 545 GLLKALHPEWSPAAIKSAIMTSATISDNTRNLILDGGSPIFAPATPFIYGSGHIHPIGAI 604

Query: 633 DPGLVYDLSPNDYLEFLCASGYKAKNFRVFGEKNFKCPISGSILNFNYPSIGVQNLTGCV 692
           DPGLVYDLSPNDYLEFLCASGYKAKN RVFGEKNFKCP+SGSILNFNYPSIGVQNLTGCV
Sbjct: 605 DPGLVYDLSPNDYLEFLCASGYKAKNIRVFGEKNFKCPVSGSILNFNYPSIGVQNLTGCV 664

Query: 693 TLTRRLKNVGRPGVYRVRVRRPKGVKVLVKPRVLKFRKIGEEKRFELTMIGAVAEGQIGY 752
           TLTRRLKNVGRPGVYRVRVRRPKGVKVLVKPRVLKFRKIGEEKRFELTMIGAVAEGQIGY
Sbjct: 665 TLTRRLKNVGRPGVYRVRVRRPKGVKVLVKPRVLKFRKIGEEKRFELTMIGAVAEGQIGY 692

Query: 753 GTLIWTDGKHFVKSPIVVSSGFF 775
           GTLIWTDGKHFVKSPIVVSSGFF
Sbjct: 725 GTLIWTDGKHFVKSPIVVSSGFF 692

BLAST of Csor.00g163510 vs. ExPASy TrEMBL
Match: A0A6J1EK27 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434038 PE=3 SV=1)

HSP 1 Score: 1481 bits (3834), Expect = 0.0
Identity = 729/753 (96.81%), Postives = 740/753 (98.27%), Query Frame = 0

Query: 23  VKKSYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGSFEKAKDAIFYSYKNHINGFA 82
           + +SYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGS EKAKDAIFYSYKNHINGFA
Sbjct: 16  ILQSYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFA 75

Query: 83  AILDEEEATNLAKHPEVATVLPNKARELHTTHSWEFMHLEKNGFIFPFSPWRKARFGKDV 142
           AILDEEEATNLAKHPEVA VLPNKA+ELHTTHSWEFMHLEKNGFIFPFSPWRKARFGKDV
Sbjct: 76  AILDEEEATNLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNGFIFPFSPWRKARFGKDV 135

Query: 143 IIANLDTGVWPESKSFGEHGITGGVPSRWKGGCTDKTPDAVPCNKKLIGAKYFNQGVIAY 202
           IIANLDTGVWPESKSFGE GITGGVPSRWKGGC DKTPDAVPCNKKLIGAKYFNQG+IAY
Sbjct: 136 IIANLDTGVWPESKSFGEQGITGGVPSRWKGGCMDKTPDAVPCNKKLIGAKYFNQGLIAY 195

Query: 203 LKSHNLTDELPLIGNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAA 262
           LKSHNLTDELPLI NSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAA
Sbjct: 196 LKSHNLTDELPLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAA 255

Query: 263 YKVCWPFRNGGGCYDADIFDGFDQAIYDGVDVLSLSIGSPPDEYYDDTIAIASFHALKKG 322
           YKVCWPFRNGGGCYDADIFDGFDQAI+DGVDVLSLSIGSPPDEYYDDTIAIASFHALKKG
Sbjct: 256 YKVCWPFRNGGGCYDADIFDGFDQAIHDGVDVLSLSIGSPPDEYYDDTIAIASFHALKKG 315

Query: 323 IPVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQAPIELGNGQRFWGLSLSRPLARR 382
           IPVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQAPIELGNGQRFWGLSLSRPLARR
Sbjct: 316 IPVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQAPIELGNGQRFWGLSLSRPLARR 375

Query: 383 KLYPLITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVVAGA 442
           KLYPLITGAQAKATT SADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAV+AGA
Sbjct: 376 KLYPLITGAQAKATTTSADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGA 435

Query: 443 VGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLIPPSSKVNTKP 502
           VGMILCNDRFSGFKIIADLHVLPASHISYNDGQA+SSYINSRKNPMGYLIPPSSKVNTKP
Sbjct: 436 VGMILCNDRFSGFKIIADLHVLPASHISYNDGQAISSYINSRKNPMGYLIPPSSKVNTKP 495

Query: 503 SPTMAAFSSRGPNIVSPEIIKPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTS 562
           SPTMAAFSSRGPNIVSPEIIKPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTS
Sbjct: 496 SPTMAAFSSRGPNIVSPEIIKPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTS 555

Query: 563 MSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTRNLILDGGSPIFAPATPFIYG 622
           MSCPHVSGIVGLLKALHP+WSPAAIKSAIMTSATISDNT NLILDGGSPIFAPATPFIYG
Sbjct: 556 MSCPHVSGIVGLLKALHPQWSPAAIKSAIMTSATISDNTMNLILDGGSPIFAPATPFIYG 615

Query: 623 SGHIHPIGAIDPGLVYDLSPNDYLEFLCASGYKAKNFRVFGEKNFKCPISGSILNFNYPS 682
           SGHIHPIGAIDPGLVYDLSPNDYLEFLCASGYK KN RVF E+N+KCP+SGSILNFNYPS
Sbjct: 616 SGHIHPIGAIDPGLVYDLSPNDYLEFLCASGYKEKNIRVFAEENYKCPVSGSILNFNYPS 675

Query: 683 IGVQNLTGCVTLTRRLKNVGRPGVYRVRVRRPKGVKVLVKPRVLKFRKIGEEKRFELTMI 742
           IGVQNLTGCVTLTRRLKNVGRPGVYRVRVRRPKGVKVLVKPRVLKFRKIGEEKRFELTM 
Sbjct: 676 IGVQNLTGCVTLTRRLKNVGRPGVYRVRVRRPKGVKVLVKPRVLKFRKIGEEKRFELTMT 735

Query: 743 GAVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 775
           G VAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF
Sbjct: 736 GVVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 768

BLAST of Csor.00g163510 vs. ExPASy TrEMBL
Match: A0A6J1EG71 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434037 PE=3 SV=1)

HSP 1 Score: 1400 bits (3625), Expect = 0.0
Identity = 691/752 (91.89%), Postives = 717/752 (95.35%), Query Frame = 0

Query: 24   KKSYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGSFEKAKDAIFYSYKNHINGFAA 83
            K+SYIVLLGSHSHGLEVSA+D+QRVVDSHHKLLGS LGS EKAKDAIF+SYKNHINGFAA
Sbjct: 251  KESYIVLLGSHSHGLEVSAVDLQRVVDSHHKLLGSLLGSSEKAKDAIFHSYKNHINGFAA 310

Query: 84   ILDEEEATNLAKHPEVATVLPNKARELHTTHSWEFMHLEKNGFIFPFSPWRKARFGKDVI 143
            IL+EEEAT LAKHPEVA VLPNKA+ELHTTHSWEFMHLEKNG + PFSPWRKARFG+DVI
Sbjct: 311  ILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNGVVLPFSPWRKARFGRDVI 370

Query: 144  IANLDTGVWPESKSFGEHGITGGVPSRWKGGCTDKTPDAVPCNKKLIGAKYFNQGVIAYL 203
            IANLDTGVWPESKSFGE GI GGVPSRWKGGC DKTPDAVPCN+KLIGAKYFNQG+IAYL
Sbjct: 371  IANLDTGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDAVPCNRKLIGAKYFNQGLIAYL 430

Query: 204  KSHNLTDELPLIGNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAY 263
            KSHNLTDEL LI NSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAY
Sbjct: 431  KSHNLTDELSLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAY 490

Query: 264  KVCWPFRNGGGCYDADIFDGFDQAIYDGVDVLSLSIGSPPDEYYDDTIAIASFHALKKGI 323
            KVCWPF N GGCYD D+ DGFDQAI+DGVDVLSLSIGSPP EYYDDTIAIASF A+KKGI
Sbjct: 491  KVCWPFHNSGGCYDIDVVDGFDQAIHDGVDVLSLSIGSPPAEYYDDTIAIASFLAIKKGI 550

Query: 324  PVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQAPIELGNGQRFWGLSLSRPLARRK 383
            PVVCSAGNSGPSMA+A+NIAPWILTVGASTLDREFQAPIELGNGQRFWG SLSRPLARRK
Sbjct: 551  PVVCSAGNSGPSMASASNIAPWILTVGASTLDREFQAPIELGNGQRFWGSSLSRPLARRK 610

Query: 384  LYPLITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVVAGAV 443
            LYPLITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAV+AGAV
Sbjct: 611  LYPLITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAV 670

Query: 444  GMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLIPPSSKVNTKPS 503
            GMILCNDRFSGFKIIADLHVLPASHI+YNDGQAV SYINS KNPMGYLIPPSSKVNTKPS
Sbjct: 671  GMILCNDRFSGFKIIADLHVLPASHINYNDGQAVFSYINSWKNPMGYLIPPSSKVNTKPS 730

Query: 504  PTMAAFSSRGPNIVSPEIIKPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSM 563
            PTMAAFSSRGPN+VSPEIIKPDVTAPGV+IIAAFSGAVSPT EPFDNRTVPYITMSGTSM
Sbjct: 731  PTMAAFSSRGPNMVSPEIIKPDVTAPGVNIIAAFSGAVSPTREPFDNRTVPYITMSGTSM 790

Query: 564  SCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTRNLILDGGSPIFAPATPFIYGS 623
            SCPHVSGIVGLLKALHPEWSPAAIKSAIMTSA ISDNT NLILDGGSP+FAPATPFIYGS
Sbjct: 791  SCPHVSGIVGLLKALHPEWSPAAIKSAIMTSAIISDNTMNLILDGGSPMFAPATPFIYGS 850

Query: 624  GHIHPIGAIDPGLVYDLSPNDYLEFLCASGYKAKNFRVFGEKNFKCPISGSILNFNYPSI 683
            GHIHP GAIDPGLVYDLSPNDYLEFLCASGYK KN RVF ++NFKCPIS SI NFNYPSI
Sbjct: 851  GHIHPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNIRVFADENFKCPISCSIFNFNYPSI 910

Query: 684  GVQNLTGCVTLTRRLKNVGRPGVYRVRVRRPKGVKVLVKPRVLKFRKIGEEKRFELTMIG 743
            GVQNLTG VTL RRLKNVGRPGVYRVRVR+P+GVKV VKPRVLKF KIGEEKRFELTM G
Sbjct: 911  GVQNLTGSVTLKRRLKNVGRPGVYRVRVRQPEGVKVSVKPRVLKFWKIGEEKRFELTMTG 970

Query: 744  AVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 775
            A+AEGQIGYGTLIWTDGKHFV+SPIVVSSG F
Sbjct: 971  AMAEGQIGYGTLIWTDGKHFVRSPIVVSSGLF 1002

BLAST of Csor.00g163510 vs. ExPASy TrEMBL
Match: A0A6J1I7Q4 (subtilisin-like protease SBT5.4 OS=Cucurbita maxima OX=3661 GN=LOC111471617 PE=3 SV=1)

HSP 1 Score: 1400 bits (3623), Expect = 0.0
Identity = 703/775 (90.71%), Postives = 717/775 (92.52%), Query Frame = 0

Query: 1   MNLTNFILILFWFFPLISPVVAVKKSYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFL 60
           MN TNFILILFWFF LISPV+AVKKSYIVLLGSHSHGLEVS ID+QRVVDSHHKLLGSFL
Sbjct: 1   MNFTNFILILFWFFRLISPVIAVKKSYIVLLGSHSHGLEVSTIDLQRVVDSHHKLLGSFL 60

Query: 61  GSFEKAKDAIFYSYKNHINGFAAILDEEEATNLAKHPEVATVLPNKARELHTTHSWEFMH 120
           GS EKAKDAIFYSYKNHINGFAAILDEEEAT  AKHPEVA VLPNKA+ELHTTHSWEFMH
Sbjct: 61  GSSEKAKDAIFYSYKNHINGFAAILDEEEATKFAKHPEVAAVLPNKAKELHTTHSWEFMH 120

Query: 121 LEKNGFIFPFSPWRKARFGKDVIIANLDTGVWPESKSFGEHGITGGVPSRWKGGCTDKTP 180
           LEKNGFI PFSPWRKARFGKDVIIANLDTGVWPESKSFGE GI GGVPSRWKGGCTDKTP
Sbjct: 121 LEKNGFILPFSPWRKARFGKDVIIANLDTGVWPESKSFGEQGIVGGVPSRWKGGCTDKTP 180

Query: 181 DAVPCNKKLIGAKYFNQGVIAYLKSHNLTDELPLIGNSTRDYVGHGTHTLSTAGGSYVSG 240
           DAVPCN+KLIGAKYFNQGVIAYLKS NLTDELPLI NSTRDYVGHG+HTLSTAGG+YVSG
Sbjct: 181 DAVPCNRKLIGAKYFNQGVIAYLKSQNLTDELPLIVNSTRDYVGHGSHTLSTAGGNYVSG 240

Query: 241 VSVFGSGIGTAKGGSPKARVAAYKVCWPFRNGGGCYDADIFDGFDQAIYDGVDVLSLSIG 300
           VSVFGSGIGTAKGGSPKARVAAYKVCWPF N GGCYDADIFDG DQAIYDGVDVLSLSIG
Sbjct: 241 VSVFGSGIGTAKGGSPKARVAAYKVCWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLSIG 300

Query: 301 SPPDEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQA 360
           SPP EYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQA
Sbjct: 301 SPPAEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQA 360

Query: 361 PIELGNGQRFWGLSLSRPLARRKLYPLITGAQAKATTASADDAMLCKPKTLDHSKVNGKI 420
           PIELGNGQRFWG SLSRPLA RKLYPLITGAQAKATTASADDAMLCKPKTLD SKVNGKI
Sbjct: 361 PIELGNGQRFWGSSLSRPLAGRKLYPLITGAQAKATTASADDAMLCKPKTLDRSKVNGKI 420

Query: 421 LVCLTGGSSRIDKGMQAVVAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSY 480
           LVCLTGGSSRIDKGMQAV+AGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSY
Sbjct: 421 LVCLTGGSSRIDKGMQAVLAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSY 480

Query: 481 INSRKNPMGYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDVTAPGVDIIAAFSGA 540
           INSRKNPMGYLIPPSSKVNTKPSP MAAFSSRGPN++SPEIIKPDVTAPGV+IIAAFSGA
Sbjct: 481 INSRKNPMGYLIPPSSKVNTKPSPIMAAFSSRGPNMISPEIIKPDVTAPGVNIIAAFSGA 540

Query: 541 VSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDN 600
           VSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDN
Sbjct: 541 VSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDN 600

Query: 601 TRNLILDGGSPIFAPATPFIYGSGHIHPIGAIDPGLVYDLSPNDYLEFLCASGYKAKNFR 660
           T NL+LDGGSPIFAPATPFIYGSGHIHP GAIDPGL                        
Sbjct: 601 TMNLMLDGGSPIFAPATPFIYGSGHIHPTGAIDPGL------------------------ 660

Query: 661 VFGEKNFKCPISGSILNFNYPSIGVQNLTGCVTLTRRLKNVGRPGVYRVRVRRPKGVKVL 720
                   CP+SGSILNFNYPSIGVQNLTG VT+TRRLKNVGRPGVYRVRVRRP+GVKV 
Sbjct: 661 --------CPVSGSILNFNYPSIGVQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVS 720

Query: 721 VKPRVLKFRKIGEEKRFELTMIGAVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 775
           VKPRVLKF KIGEEKRFELTM GAVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF
Sbjct: 721 VKPRVLKFWKIGEEKRFELTMTGAVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 743

BLAST of Csor.00g163510 vs. ExPASy TrEMBL
Match: A0A6J1EG66 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434032 PE=3 SV=1)

HSP 1 Score: 1347 bits (3487), Expect = 0.0
Identity = 670/772 (86.79%), Postives = 707/772 (91.58%), Query Frame = 0

Query: 4   TNFILILFWFFPLISPVVAVKKSYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGSF 63
           TNFI +LF FF LISPV+AVKKSYIVLLGSHSHGLEVSA+D+QR VDSHHKLLGSFLGS 
Sbjct: 5   TNFIFMLFGFFWLISPVIAVKKSYIVLLGSHSHGLEVSAVDLQRAVDSHHKLLGSFLGSS 64

Query: 64  EKAKDAIFYSYKNHINGFAAILDEEEATNLAKHPEVATVLPNKARELHTTHSWEFMHLEK 123
            KAKDAIFYSYKNHINGFAAIL+EEEAT LAKHPEVA VLPNKA+ELHTTHSWE MHLEK
Sbjct: 65  GKAKDAIFYSYKNHINGFAAILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWESMHLEK 124

Query: 124 NGFIFPFSPWRKARFGKDVIIANLDTGVWPESKSFGEHGITGGVPSRWKGGCTDKTPDAV 183
           N  I   SPWRKARFG+DVIIANLD+GVWPESKSFGE GI GGVPSRWKGGC DKTPD V
Sbjct: 125 NDVIPSSSPWRKARFGEDVIIANLDSGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDGV 184

Query: 184 PCNKKLIGAKYFNQGVIAYLKSHNLTDELPLIGNSTRDYVGHGTHTLSTAGGSYVSGVSV 243
           PCN+KLIGAKYFNQG +AYLKS NL +ELPLI NSTRDY GHG+HTLSTAGGSYVS VSV
Sbjct: 185 PCNRKLIGAKYFNQGALAYLKSQNLMEELPLIVNSTRDYKGHGSHTLSTAGGSYVSNVSV 244

Query: 244 FGSGIGTAKGGSPKARVAAYKVCWPFRNGGGCYDADIFDGFDQAIYDGVDVLSLSIGSPP 303
           FGSGIGTAKGGSPKARVAAYKVCWP  N GGC+DAD+ + FD AI+DGVDVLSLSIGSPP
Sbjct: 245 FGSGIGTAKGGSPKARVAAYKVCWPLPNIGGCFDADVAEAFDHAIHDGVDVLSLSIGSPP 304

Query: 304 DEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQAPIE 363
            EYYDD IAI SFHALKKGIPVVCSAGNSGPSMATA+NIAPWILTVGASTLDREFQAPIE
Sbjct: 305 AEYYDDIIAIGSFHALKKGIPVVCSAGNSGPSMATASNIAPWILTVGASTLDREFQAPIE 364

Query: 364 LGNGQRFWGLSLSRPLARRKLYPLITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVC 423
           LGNGQRF G SLS PLA RKLYPLITGAQAKATTA A D++LCKP+TLDHSKV GKILVC
Sbjct: 365 LGNGQRFTGSSLSNPLAGRKLYPLITGAQAKATTAFAQDSILCKPETLDHSKVKGKILVC 424

Query: 424 LTGGSSRIDKGMQAVVAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINS 483
           L GGSSRIDKGMQA++AGAVGMILCNDR SGF+I+ADLHVLP SHI+YNDGQAV SYI S
Sbjct: 425 LRGGSSRIDKGMQALLAGAVGMILCNDRLSGFEIMADLHVLPTSHINYNDGQAVFSYIYS 484

Query: 484 RKNPMGYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDVTAPGVDIIAAFSGAVSP 543
            KNPMGYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDVTAPGV+IIAAFSGAVSP
Sbjct: 485 TKNPMGYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDVTAPGVNIIAAFSGAVSP 544

Query: 544 TGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTRN 603
           TGEPFDNR VPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSA +SDNT N
Sbjct: 545 TGEPFDNRAVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSARVSDNTMN 604

Query: 604 LILDGGSPIFAPATPFIYGSGHIHPIGAIDPGLVYDLSPNDYLEFLCASGYKAKNFRVFG 663
           L+LDGGSPIFAPATPF+YGSGHI P GAIDPGLVYDLSPNDYLEFLCASGY  KN RVF 
Sbjct: 605 LMLDGGSPIFAPATPFVYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYTEKNLRVFT 664

Query: 664 EKNFKCPISGSILNFNYPSIGVQNLTGCVTLTRRLKNVGRPGVYRVRVRRPKGVKVLVKP 723
           + NFKCP+SGSILNFNYPSIGVQNLTG VT++RRLKNVG PGVYRVRV++P+GVKV VKP
Sbjct: 665 DGNFKCPVSGSILNFNYPSIGVQNLTGSVTVSRRLKNVGMPGVYRVRVQQPEGVKVSVKP 724

Query: 724 RVLKFRKIGEEKRFELTMIGAVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 775
            VLKF KIGEEK FELTM GAVAEGQIGYGTLIWTD KHFV+SPIVVSS FF
Sbjct: 725 NVLKFEKIGEEKGFELTMTGAVAEGQIGYGTLIWTDDKHFVRSPIVVSSSFF 776

BLAST of Csor.00g163510 vs. ExPASy TrEMBL
Match: A0A6J1EGQ8 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434031 PE=3 SV=1)

HSP 1 Score: 1308 bits (3384), Expect = 0.0
Identity = 647/752 (86.04%), Postives = 687/752 (91.36%), Query Frame = 0

Query: 24  KKSYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGSFEKAKDAIFYSYKNHINGFAA 83
           ++SYIVLLGSH+HGLEVSA+D+QRVVDSHHKLLGSFLGS EKAKDAIFYSYKNHINGFAA
Sbjct: 2   EESYIVLLGSHAHGLEVSAVDLQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAA 61

Query: 84  ILDEEEATNLAKHPEVATVLPNKARELHTTHSWEFMHLEKNGFIFPFSPWRKARFGKDVI 143
           ILDEEEAT LAKHPE+A VLPNKA+ELHTT SWEFMHLEKNG   P SPWRKAR G++VI
Sbjct: 62  ILDEEEATKLAKHPEIAAVLPNKAKELHTTRSWEFMHLEKNGVSPPSSPWRKARSGQNVI 121

Query: 144 IANLDTGVWPESKSFGEHGITGGVPSRWKGGCTDKTPDAVPCNKKLIGAKYFNQGVIAYL 203
           IANLDTGVWPESKSFGEHGI GGVPS+WKGGC DKTPDAVPCNKKLIGAKYFN GVIAYL
Sbjct: 122 IANLDTGVWPESKSFGEHGIVGGVPSKWKGGCMDKTPDAVPCNKKLIGAKYFNHGVIAYL 181

Query: 204 KSHNLTDELPLIGNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAY 263
           KS NLT EL  I NSTRDY GHG+HTLSTAGGSYVSGVSVFGSGIGTAKGGSPKA VAAY
Sbjct: 182 KSENLTKELSFIVNSTRDYEGHGSHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKAHVAAY 241

Query: 264 KVCWPFRNGGGCYDADIFDGFDQAIYDGVDVLSLSIGSPPDEYYDDTIAIASFHALKKGI 323
           KVCWP  + GGC+DADI   FD AI+DGVDVLS+S+GSPP EYY+D IAIASFHALKKGI
Sbjct: 242 KVCWPLHDTGGCFDADIAKAFDHAIHDGVDVLSVSLGSPPAEYYNDIIAIASFHALKKGI 301

Query: 324 PVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQAPIELGNGQRFWGLSLSRPLARRK 383
           PVVCSAGN GPSMAT +N APWILTVGASTLDREFQAPIEL NG+ F G SLS PL+ RK
Sbjct: 302 PVVCSAGNDGPSMATVSNTAPWILTVGASTLDREFQAPIELRNGKHFKGSSLSGPLSGRK 361

Query: 384 LYPLITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVVAGAV 443
           LYPLITGAQAKATTAS DDAMLCKPKTLDHSKV GKILVCL G SSR+DKG+QA++AGAV
Sbjct: 362 LYPLITGAQAKATTASTDDAMLCKPKTLDHSKVKGKILVCLRGDSSRVDKGVQALLAGAV 421

Query: 444 GMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLIPPSSKVNTKPS 503
           GMILCNDR SGF+IIAD HVLPASHISYNDGQAVSSYINS KNPMG+LIPP SKVNTKPS
Sbjct: 422 GMILCNDRLSGFEIIADPHVLPASHISYNDGQAVSSYINSTKNPMGHLIPPLSKVNTKPS 481

Query: 504 PTMAAFSSRGPNIVSPEIIKPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSM 563
           PTMAAFSSRGPN+VSPEIIKPDVTAPGV+IIAAFSG+VSPTGEPFDNRTVPYITMSGTSM
Sbjct: 482 PTMAAFSSRGPNMVSPEIIKPDVTAPGVNIIAAFSGSVSPTGEPFDNRTVPYITMSGTSM 541

Query: 564 SCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTRNLILDGGSPIFAPATPFIYGS 623
           SCPHVSGIVGL+KALHP+WSPAAIKSAIMTSA I DNT N++LDGGSPIFA AT FI+GS
Sbjct: 542 SCPHVSGIVGLVKALHPKWSPAAIKSAIMTSARIRDNTMNIMLDGGSPIFASATAFIFGS 601

Query: 624 GHIHPIGAIDPGLVYDLSPNDYLEFLCASGYKAKNFRVFGEKNFKCPISGSILNFNYPSI 683
           GHI P GAIDPGLVYDLSPNDYLEFLCASGYK KN RVF + NFKCP+S SILNFNYPSI
Sbjct: 602 GHIRPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNLRVFADGNFKCPVSSSILNFNYPSI 661

Query: 684 GVQNLTGCVTLTRRLKNVGRPGVYRVRVRRPKGVKVLVKPRVLKFRKIGEEKRFELTMIG 743
           GVQNLTG VTLTRRLKNVGRPGVYRVRV++P+GVKV VKP VLKF KIGEEKRFELTM G
Sbjct: 662 GVQNLTGNVTLTRRLKNVGRPGVYRVRVQQPEGVKVSVKPSVLKFEKIGEEKRFELTMTG 721

Query: 744 AVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 775
            VA GQIGYGTLIWTDGKH V+SPIVVSSGFF
Sbjct: 722 VVANGQIGYGTLIWTDGKHLVRSPIVVSSGFF 753

BLAST of Csor.00g163510 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 859.4 bits (2219), Expect = 2.3e-249
Identity = 437/773 (56.53%), Postives = 547/773 (70.76%), Query Frame = 0

Query: 2   NLTNFILILFWFFPLISPVVAVKKSYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLG 61
           +L++ +L++  FF   SP  A+KKSYIV LGSH+H  ++S+  +  V  SH   L SF+G
Sbjct: 20  SLSSLLLLVTLFF---SPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVG 79

Query: 62  SFEKAKDAIFYSYKNHINGFAAILDEEEATNLAKHPEVATVLPNKARELHTTHSWEFMHL 121
           S E AK+AIFYSYK HINGFAAILDE EA  +AKHP+V +V PNK R+LHTTHSW FM L
Sbjct: 80  SHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLL 139

Query: 122 EKNGFIFPFSPWRKARFGKDVIIANLDTGVWPESKSFGEHGITGGVPSRWKGGCTDKTPD 181
            KNG +   S W KA +G+D IIANLDTGVWPESKSF + G  G VP+RWKG C      
Sbjct: 140 AKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGY-GAVPARWKGRCHKD--- 199

Query: 182 AVPCNKKLIGAKYFNQGVIAYL-KSHNLTDELPLIGNSTRDYVGHGTHTLSTAGGSYVSG 241
            VPCN+KLIGA+YFN+G +AY     N + E      + RD+ GHG+HTLSTA G++V G
Sbjct: 200 -VPCNRKLIGARYFNKGYLAYTGLPSNASYE------TCRDHDGHGSHTLSTAAGNFVPG 259

Query: 242 VSVFGSGIGTAKGGSPKARVAAYKVCWPFRNGGGCYDADIFDGFDQAIYDGVDVLSLSIG 301
            +VFG G GTA GGSPKARVAAYKVCWP  +G  C+DADI    + AI DGVDVLS S+G
Sbjct: 260 ANVFGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVG 319

Query: 302 SPPDEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQA 361
               +Y  D IAI SFHA+K G+ VVCSAGNSGP   T +N+APW++TVGAS++DREFQA
Sbjct: 320 GDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQA 379

Query: 362 PIELGNGQRFWGLSLSRPLARRKLYPLITGAQAKATTASADDAMLCKPKTLDHSKVNGKI 421
            +EL NGQ F G SLS+PL   K+Y LI+ A A     +  DA+LCK  +LD  KV GKI
Sbjct: 380 FVELKNGQSFKGTSLSKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKI 439

Query: 422 LVCLTGGSSRIDKGMQAVVAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSY 481
           LVCL G ++R+DKGMQA  AGA GM+LCND+ SG +II+D HVLPAS I Y DG+ + SY
Sbjct: 440 LVCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSY 499

Query: 482 INSRKNPMGYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDVTAPGVDIIAAFSGA 541
           ++S K+P GY+  P++ +NTKP+P MA+FSSRGPN ++P I+KPD+TAPGV+IIAAF+ A
Sbjct: 500 LSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEA 559

Query: 542 VSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDN 601
             PT    DNR  P+ T SGTSMSCPH+SG+VGLLK LHP WSPAAI+SAIMT++   +N
Sbjct: 560 TGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNN 619

Query: 602 TRNLILDGGSPIFAPATPFIYGSGHIHPIGAIDPGLVYDLSPNDYLEFLCASGYKAKNFR 661
            R  ++D     F  A PF YGSGH+ P  A  PGLVYDL+  DYL+FLCA GY     +
Sbjct: 620 RRKPMVDES---FKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQ 679

Query: 662 VFGE-KNFKCPISGSILNFNYPSIGVQNLTGCVTLTRRLKNVGRPGVYRVRVRRPKGVKV 721
           +F E   + C    ++L+FNYPSI V NLTG +T+TR+LKNVG P  Y  R R P GV+V
Sbjct: 680 LFAEDPQYTCRQGANLLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFREPLGVRV 739

Query: 722 LVKPRVLKFRKIGEEKRFELTM--IGAVAEGQIGYGTLIWTDGKHFVKSPIVV 771
            V+P+ L F K GE K F++T+  +     G + +G L WTD  H+V+SPIVV
Sbjct: 740 SVEPKQLTFNKTGEVKIFQMTLRPLPVTPSGYV-FGELTWTDSHHYVRSPIVV 774

BLAST of Csor.00g163510 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 793.9 bits (2049), Expect = 1.2e-229
Identity = 410/774 (52.97%), Postives = 537/774 (69.38%), Query Frame = 0

Query: 5   NFILILFWFFPLISPVVAVK--KSYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGS 64
           +F+L+L         ++A K   SY+V  G+HSH  E++   + RV ++H+  LGSF GS
Sbjct: 8   SFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGS 67

Query: 65  FEKAKDAIFYSYKNHINGFAAILDEEEATNLAKHPEVATVLPNKARELHTTHSWEFMHLE 124
            E+A DAIFYSY  HINGFAA LD + A  ++KHPEV +V PNKA +LHTT SW+F+ LE
Sbjct: 68  RERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLE 127

Query: 125 KNGFIFPFSPWRKARFGKDVIIANLDTGVWPESKSFGEHGITGGVPSRWKGGCTDKTPDA 184
            N ++   S WRKARFG+D IIANLDTGVWPESKSF + G+ G +PSRWKG C ++    
Sbjct: 128 HNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGL-GPIPSRWKGICQNQKDAT 187

Query: 185 VPCNKKLIGAKYFNQGVIAYLKSHNLTDELPLIGNSTRDYVGHGTHTLSTAGGSYVSGVS 244
             CN+KLIGA+YFN+G  A +   N + + P      RD  GHG+HTLSTA G +V GVS
Sbjct: 188 FHCNRKLIGARYFNKGYAAAVGHLNSSFDSP------RDLDGHGSHTLSTAAGDFVPGVS 247

Query: 245 VFGSGIGTAKGGSPKARVAAYKVCWPFRNGGGCYDADIFDGFDQAIYDGVDVLSLSIGSP 304
           +FG G GTAKGGSP+ARVAAYKVCWP   G  CYDAD+   FD AI+DG DV+S+S+G  
Sbjct: 248 IFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGE 307

Query: 305 PDEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQAPI 364
           P  +++D++AI SFHA KK I VVCSAGNSGP+ +T +N+APW +TVGAST+DREF + +
Sbjct: 308 PTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNL 367

Query: 365 ELGNGQRFWGLSL-SRPLARRKLYPLITGAQAKATTASADDAMLCKPKTLDHSKVNGKIL 424
            LGNG+ + G SL S  L   K YP++    AKA  ASA DA LCK  +LD  K  GKIL
Sbjct: 368 VLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKIL 427

Query: 425 VCLTGGSSRIDKGMQAVVAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYI 484
           VCL G + R++KG    + G +GM+L N   +G  ++AD HVLPA+ ++  D  AVS YI
Sbjct: 428 VCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYI 487

Query: 485 NSRKNPMGYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDVTAPGVDIIAAFSGAV 544
           +  K P+ ++ P  + +  KP+P MA+FSS+GP+IV+P+I+KPD+TAPGV +IAA++GAV
Sbjct: 488 SQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAV 547

Query: 545 SPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNT 604
           SPT E FD R + +  +SGTSMSCPH+SGI GLLK  +P WSPAAI+SAIMT+ATI D+ 
Sbjct: 548 SPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDI 607

Query: 605 RNLILDGGSPIFAPATPFIYGSGHIHPIGAIDPGLVYDLSPNDYLEFLCASGYKAKNFRV 664
              I +  +     ATPF +G+GH+ P  A++PGLVYDL   DYL FLC+ GY A    V
Sbjct: 608 PGPIQNATN---MKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISV 667

Query: 665 FGEKNFKCPISG-SILNFNYPSIGVQNLTGC-VTLTRRLKNVGRPGVYRVRVRRPKGVKV 724
           F   NF C     S++N NYPSI V NLT   VT++R +KNVGRP +Y V+V  P+GV V
Sbjct: 668 FSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYV 727

Query: 725 LVKPRVLKFRKIGEEKRFELTMI---GAVAEGQIGYGTLIWTDGKHFVKSPIVV 771
            VKP  L F K+GE+K F++ ++   G VA+G + +G L+W+D KH V+SPIVV
Sbjct: 728 AVKPTSLNFTKVGEQKTFKVILVKSKGNVAKGYV-FGELVWSDKKHRVRSPIVV 770

BLAST of Csor.00g163510 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 563.5 bits (1451), Expect = 2.6e-160
Identity = 334/782 (42.71%), Postives = 465/782 (59.46%), Query Frame = 0

Query: 1   MNLTNFILILFWFFPLISPVVAVKKSYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFL 60
           ++ T F L+L   F  +S   + + +YIV          ++   +    D H     S L
Sbjct: 6   LSSTAFFLLLCLGFCHVSSSSSDQGTYIV---------HMAKSQMPSSFDLHSNWYDSSL 65

Query: 61  GSFEKAKDAIFYSYKNHINGFAAILDEEEATNLAKHPEVATVLPNKARELHTTHSWEFMH 120
            S   + + + Y+Y+N I+GF+  L +EEA +L   P V +VLP    ELHTT +  F+ 
Sbjct: 66  RSISDSAE-LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLG 125

Query: 121 L-EKNGFIFPFSPWRKARFGKDVIIANLDTGVWPESKSFGEHGITGGVPSRWKGGCTDKT 180
           L E    +FP     +A    DV++  LDTGVWPESKS+ + G  G +PS WKGGC   T
Sbjct: 126 LDEHTADLFP-----EAGSYSDVVVGVLDTGVWPESKSYSDEGF-GPIPSSWKGGCEAGT 185

Query: 181 P-DAVPCNKKLIGAKYFNQGVIAYLKSHNLTDELPLIGNSTRDYVGHGTHTLSTAGGSYV 240
              A  CN+KLIGA++F +G  + +   + + E      S RD  GHGTHT STA GS V
Sbjct: 186 NFTASLCNRKLIGARFFARGYESTMGPIDESKE----SRSPRDDDGHGTHTSSTAAGSVV 245

Query: 241 SGVSVFGSGIGTAKGGSPKARVAAYKVCWPFRNGGGCYDADIFDGFDQAIYDGVDVLSLS 300
            G S+ G   GTA+G +P+ARVA YKVCW     GGC+ +DI    D+AI D V+VLS+S
Sbjct: 246 EGASLLGYASGTARGMAPRARVAVYKVCWL----GGCFSSDILAAIDKAIADNVNVLSMS 305

Query: 301 IGSPPDEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDREF 360
           +G    +YY D +AI +F A+++GI V CSAGN+GPS ++ +N+APWI TVGA TLDR+F
Sbjct: 306 LGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDF 365

Query: 361 QAPIELGNGQRFWGLSLSRPLA-RRKLYPLITGAQAKATTASADDAMLCKPKTLDHSKVN 420
            A   LGNG+ F G+SL +  A   KL P I    A    ++A +  LC   TL   KV 
Sbjct: 366 PALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNA----SNATNGNLCMTGTLIPEKVK 425

Query: 421 GKILVCLTGGSSRIDKGMQAVVAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAV 480
           GKI++C  G ++R+ KG     AG VGMIL N   +G +++AD H+LPA+ +    G  +
Sbjct: 426 GKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDII 485

Query: 481 SSYINSRKNPMGYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDVTAPGVDIIAAF 540
             Y+ +  NP   +    + V  KPSP +AAFSSRGPN ++P I+KPD+ APGV+I+AA+
Sbjct: 486 RHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAW 545

Query: 541 SGAVSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSA-- 600
           +GA  PTG   D+R V +  +SGTSMSCPHVSG+  LLK++HPEWSPAAI+SA+MT+A  
Sbjct: 546 TGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYK 605

Query: 601 TISDNTRNLILDGGSPIFAPATPFIYGSGHIHPIGAIDPGLVYDLSPNDYLEFLCASGYK 660
           T  D    L +  G     P+TPF +G+GH+ P  A +PGL+YDL+  DYL FLCA  Y 
Sbjct: 606 TYKDGKPLLDIATGK----PSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYT 665

Query: 661 AKNFRVFGEKNFKCPISG--SILNFNYPSIGVQ-NLTGCVTLTRRLKNVGRPGVYRVRV- 720
           +   R    +N+ C  S   S+ + NYPS  V  +  G    TR + +VG  G Y V+V 
Sbjct: 666 SPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVT 725

Query: 721 RRPKGVKVLVKPRVLKFRKIGEEKRFELTMI--GAVAEGQIGYGTLIWTDGKHFVKSPIV 772
               GVK+ V+P VL F++  E+K + +T     +   G   +G++ W+DGKH V SP+ 
Sbjct: 726 SETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVA 755

BLAST of Csor.00g163510 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 536.2 bits (1380), Expect = 4.4e-152
Identity = 336/805 (41.74%), Postives = 458/805 (56.89%), Query Frame = 0

Query: 10  LFWFFPLISPVVAVKKSYIVLLGSHSHGLEVSAIDVQR-----VVDSHHKLLGSFLGSFE 69
           +F+ FPL+         +     S S GLE   + VQR     +  SH+    S L S  
Sbjct: 9   IFFVFPLL-------LCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLP 68

Query: 70  KAKD--AIFYSYKNHINGFAAILDEEEATNLAKHPEVATVLPNKARELHTTHSWEFMHLE 129
            +     + YSY   ++GF+A L   +   L +HP V +V+P++ARE+HTTH+  F+   
Sbjct: 69  SSPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFS 128

Query: 130 KNGFIFPFSPWRKARFGKDVIIANLDTGVWPESKSFGEHGITGGVPSRWKGGCTDKTPD- 189
           +N  +     W  + +G+DVI+  LDTG+WPE  SF + G+ G +PS WKG C +  PD 
Sbjct: 129 QNSGL-----WSNSNYGEDVIVGVLDTGIWPEHPSFSDSGL-GPIPSTWKGEC-EIGPDF 188

Query: 190 -AVPCNKKLIGAKYFNQGVIAYLKSHNLTDE-LPLIGNSTRDYVGHGTHTLSTAGGSYVS 249
            A  CN+KLIGA+ F +G   YL   N T +       S RD  GHGTHT STA GS V+
Sbjct: 189 PASSCNRKLIGARAFYRG---YLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVA 248

Query: 250 GVSVFGSGIGTAKGGSPKARVAAYKVCWPFRNGGGCYDADIFDGFDQAIYDGVDVLSLSI 309
             S++    GTA G + KAR+AAYK+CW     GGCYD+DI    DQA+ DGV V+SLS+
Sbjct: 249 NASLYQYARGTATGMASKARIAAYKICWT----GGCYDSDILAAMDQAVADGVHVISLSV 308

Query: 310 GS--PPDEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDRE 369
           G+     EY+ D+IAI +F A + GI V CSAGNSGP+  TATNIAPWILTVGAST+DRE
Sbjct: 309 GASGSAPEYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDRE 368

Query: 370 FQAPIELGNGQRFWGLSLSRPLARRKLYPLITGAQAKATTASADDAMLCKPKTLDHSKVN 429
           F A    G+G+ F G SL    +       +  +Q     +    + LC P  L+ S V 
Sbjct: 369 FAANAITGDGKVFTGTSLYAGES-------LPDSQLSLVYSGDCGSRLCYPGKLNSSLVE 428

Query: 430 GKILVCLTGGSSRIDKGMQAVVAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAV 489
           GKI++C  GG++R++KG    +AG  GMIL N   SG ++ AD H++PA+ +    G  +
Sbjct: 429 GKIVLCDRGGNARVEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQI 488

Query: 490 SSYINSRKNP------MGYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDVTAPGV 549
             YI +  +P      +G LI PS      PSP +AAFSSRGPN ++P I+KPDV APGV
Sbjct: 489 RDYIKTSDSPTAKISFLGTLIGPS-----PPSPRVAAFSSRGPNHLTPVILKPDVIAPGV 548

Query: 550 DIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAI 609
           +I+A ++G V PT    D R V +  +SGTSMSCPHVSG+  LL+  HP+WSPAAIKSA+
Sbjct: 549 NILAGWTGMVGPTDLDIDPRRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSAL 608

Query: 610 MTSATISDNTRNLILDGGSPIFAPAT-----PFIYGSGHIHPIGAIDPGLVYDLSPNDYL 669
           +T+A   +N+       G PI   AT      FI+G+GH+ P  A++PGLVYD+   +Y+
Sbjct: 609 VTTAYDVENS-------GEPIEDLATGKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYV 668

Query: 670 EFLCASGYKAKNFRVFGE--------KNFKCPISGSILNFNYPSIGV--QNLTGCVTLTR 729
            FLCA GY+     VF +        +  K   +G +   NYPS  V   +    V   R
Sbjct: 669 AFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDL---NYPSFSVVFASTGEVVKYKR 728

Query: 730 RLKNVGR--PGVYRVRVRRPKGVKVLVKPRVLKFRKIGEEKRFELTMIGAVAEGQIG--- 774
            +KNVG     VY V V+ P  V++ V P  L F K      +E+T    V  G +G   
Sbjct: 729 VVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVP 770

BLAST of Csor.00g163510 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 530.8 bits (1366), Expect = 1.8e-150
Identity = 327/794 (41.18%), Postives = 461/794 (58.06%), Query Frame = 0

Query: 1   MNLTNFILILFWFFPLISPVVA-VKKSYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSF 60
           M  +  +L+LF  FP IS   +   K++I  +   S         +  +  +H+      
Sbjct: 1   MASSTIVLLLFLSFPFISFAASQAAKTFIFRIDGGS---------MPSIFPTHYHWY--- 60

Query: 61  LGSFEKAKDA-IFYSYKNHINGFAAILDEEEATNLAKHPEVATVLPNKARELHTTHSWEF 120
             S E A+++ I + Y    +GF+A++  +EA NL  HP V  V  ++ RELHTT S +F
Sbjct: 61  --STEFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQF 120

Query: 121 MHLEKNGFIFPFSPWRKARFGKDVIIANLDTGVWPESKSFGEHGITGGVPSRWKGGCTDK 180
           + L+    +     W ++ +G DVII   DTG+WPE +SF +  + G +P RW+G C   
Sbjct: 121 LGLQNQKGL-----WSESDYGSDVIIGVFDTGIWPERRSFSDLNL-GPIPKRWRGVCESG 180

Query: 181 TP-DAVPCNKKLIGAKYFNQG-VIAYLKSHNLTDELPLIGNSTRDYVGHGTHTLSTAGGS 240
                  CN+K+IGA++F +G   A +   N T E      S RD  GHGTHT STA G 
Sbjct: 181 ARFSPRNCNRKIIGARFFAKGQQAAVIGGINKTVEF----LSPRDADGHGTHTSSTAAGR 240

Query: 241 YVSGVSVFGSGIGTAKGGSPKARVAAYKVCWPFRNGGGCYDADIFDGFDQAIYDGVDVLS 300
           +    S+ G   G AKG +PKAR+AAYKVCW      GC D+DI   FD A+ DGVDV+S
Sbjct: 241 HAFKASMSGYASGVAKGVAPKARIAAYKVCW---KDSGCLDSDILAAFDAAVRDGVDVIS 300

Query: 301 LSIGSP---PDEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGAST 360
           +SIG        YY D IAI S+ A  KGI V  SAGN GP+  + TN+APW+ TVGAST
Sbjct: 301 ISIGGGDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGAST 360

Query: 361 LDREFQAPIELGNGQRFWGLSL--SRPLARRKLYPLITGAQAKATTASADDAMLCKPKTL 420
           +DR F A   LG+G R  G+SL    PL  R ++P++   ++  ++AS     LC   TL
Sbjct: 361 IDRNFPADAILGDGHRLRGVSLYAGVPLNGR-MFPVVYPGKSGMSSAS-----LCMENTL 420

Query: 421 DHSKVNGKILVCLTGGSSRIDKGMQAVVAGAVGMILCNDRFSGFKIIADLHVLPASHISY 480
           D  +V GKI++C  G S R+ KG+    AG VGMIL N   +G  ++ D H++PA  +  
Sbjct: 421 DPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGS 480

Query: 481 NDGQAVSSYINSRKNPMGYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDVTAPGV 540
           N+G  + +Y +S  NP+  +    + V  KP+P +A+FS RGPN +SPEI+KPD+ APGV
Sbjct: 481 NEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGV 540

Query: 541 DIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAI 600
           +I+AA++ AV PTG P D R   +  +SGTSM+CPHVSG   LLK+ HP+WSPA I+SA+
Sbjct: 541 NILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAM 600

Query: 601 MTSATISDNTRNLILDGGSPIFAPATPFIYGSGHIHPIGAIDPGLVYDLSPNDYLEFLCA 660
           MT+  + DN+   ++D  +     ATP+ YGSGH++   A++PGLVYD++ +DY+ FLC+
Sbjct: 601 MTTTNLVDNSNRSLIDESTG--KSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCS 660

Query: 661 SGYKAKNFRVFGEKNFKCPIS--GSILNFNYPSIGV---QNLTGCV--TLTRRLKNVGR- 720
            GY  K  +V      +CP +   S  N NYPSI      N  G V  T+ R   NVG+ 
Sbjct: 661 IGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQA 720

Query: 721 PGVYRVRVRRPKGVKVLVKPRVLKFRKIGEEKRFELTM---IGAVAEGQIG--YGTLIWT 772
             VYR R+  P+GV V VKP  L F    + + + +T+      V  G+ G  +G++ W 
Sbjct: 721 EAVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWF 759