Csor.00g163430 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g163430
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
LocationCsor_Chr13: 6506598 .. 6508076 (-)
RNA-Seq ExpressionCsor.00g163430
SyntenyCsor.00g163430
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintronterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCGTCTCTTTGCTTCCCACCGCTGCAAGAAGGTAACCTTATTTTCTCTCAAAACCTAATCTCGGTCGGTTTTTGGCCACATCGACATGATTCAATTCAAGTCGTATTCGTTTTATTTTTTCTCTTTGTCGCCTCACACCAATGGAGATAACCTACCTGAGACGAGTCCCGGCTCGACTCATAATTTTTTATTCGATTTCTGAGGATTCTATTGACATGGCTAAGTTTAGGACATGACTTTGATACCATGTTAGAAATCACGACTCTCCATAATGGTATGATATTGTCTACTTTGAATATAAGCTGTTCGATATTTGAGGATTTTATTGACATGACTAAGTTTAGAAGATGACTCTGATACCATGTTAGAAATCACGACTCTCCATAGTGGTATGATATTGTCCACTTTGAGTATAAGTGCTTGTGGCTTTGTTTTGGGCTTCCTCAAGAGGCCTCATACGAATGAAGATGGTATTCCTCAATTATAAATCCATGATCTTCCACTCAATTAACGAACGTGGGACCCACTCCCAATAATCCTTAACAATCCTCAACCGACCATTAAGATTTTTTTTTTAATAATTTTTTAATAAATATTATATTTTTTTTTGAAAGGAATAACAAACCAGAATGGATGGATATTTGTTTTCATTGAAAAATTACTTACAACAATACAATCATTTGATTAGGCTAGAAGGCAAAGTGGCCGTAATCTTGGGCGCAGCCAGAGGAATTGGGGAGTCCACAGCAAGAACCTTCTTTAAGCATGGAGCAAAACTGGTGATTGCAGACATCCTAGACGATCTCGGTCACTCTCTTTCCAACCATCTCTGTTCTTCGTCATCTTCATTCGTCCACTGCGACGTAACGAAAGAGAAAGACGTCGAAAATGCGGTTAACACAGCCATTTCCAAGTACGGAAAGTTGGACATCATGTTGAACAACGCAGGCATTCTTGGAAATCCTATATTCAACATTCTTGAAAACGAATCATGTGACTTTCAAAAGGTACTCAACGTCAACTTACTTGGGACGTTTTTCGGCACCAAGCATGCAGCAAGGGCAATGATTCCAGCTCGTCAAGGTAGCATAATCATGATGGCAAGTGTTTGCTCGACAATTGGTGGGATTGGGCCATATGGGTACACGAGCTCAAAGCATGGCGTGTTGGGATTGATGAAGAATGCAACGGTTGATTTAGGACGATATGGGATAAGGGTTAATTGTGTGTCACCCCATGTGGTGCCCTCGGACATAACTAGAGAGCTGTTTAAGATTGAAGATGGCAATGAGTTTCCTAATGTTTATTCGCATTTCAAATGTGGAGATATCTTAAGAGCAGAAGATATAGCTGAAACCGCTTTGTATTTAGCAAGTGATGCATCAAGGTTCGTAAGTGGACATAACTTAATTGTTGATGGAGGCTTCACTGCAGCAAACCAAGGTTTTTGCTCCTATAAACAATTTGATAACTAA

mRNA sequence

ATGAGCGTCTCTTTGCTTCCCACCGCTGCAAGAAGGCTAGAAGGCAAAGTGGCCGTAATCTTGGGCGCAGCCAGAGGAATTGGGGAGTCCACAGCAAGAACCTTCTTTAAGCATGGAGCAAAACTGGTGATTGCAGACATCCTAGACGATCTCGGTCACTCTCTTTCCAACCATCTCTGTTCTTCGTCATCTTCATTCGTCCACTGCGACGTAACGAAAGAGAAAGACGTCGAAAATGCGGTTAACACAGCCATTTCCAAGTACGGAAAGTTGGACATCATGTTGAACAACGCAGGCATTCTTGGAAATCCTATATTCAACATTCTTGAAAACGAATCATGTGACTTTCAAAAGGTACTCAACGTCAACTTACTTGGGACGTTTTTCGGCACCAAGCATGCAGCAAGGGCAATGATTCCAGCTCGTCAAGGTAGCATAATCATGATGGCAAGTGTTTGCTCGACAATTGGTGGGATTGGGCCATATGGGTACACGAGCTCAAAGCATGGCGTGTTGGGATTGATGAAGAATGCAACGGTTGATTTAGGACGATATGGGATAAGGGTTAATTGTGTGTCACCCCATGTGGTGCCCTCGGACATAACTAGAGAGCTGTTTAAGATTGAAGATGGCAATGAGTTTCCTAATGTTTATTCGCATTTCAAATGTGGAGATATCTTAAGAGCAGAAGATATAGCTGAAACCGCTTTGTATTTAGCAAGTGATGCATCAAGGTTCGTAAGTGGACATAACTTAATTGTTGATGGAGGCTTCACTGCAGCAAACCAAGGTTTTTGCTCCTATAAACAATTTGATAACTAA

Coding sequence (CDS)

ATGAGCGTCTCTTTGCTTCCCACCGCTGCAAGAAGGCTAGAAGGCAAAGTGGCCGTAATCTTGGGCGCAGCCAGAGGAATTGGGGAGTCCACAGCAAGAACCTTCTTTAAGCATGGAGCAAAACTGGTGATTGCAGACATCCTAGACGATCTCGGTCACTCTCTTTCCAACCATCTCTGTTCTTCGTCATCTTCATTCGTCCACTGCGACGTAACGAAAGAGAAAGACGTCGAAAATGCGGTTAACACAGCCATTTCCAAGTACGGAAAGTTGGACATCATGTTGAACAACGCAGGCATTCTTGGAAATCCTATATTCAACATTCTTGAAAACGAATCATGTGACTTTCAAAAGGTACTCAACGTCAACTTACTTGGGACGTTTTTCGGCACCAAGCATGCAGCAAGGGCAATGATTCCAGCTCGTCAAGGTAGCATAATCATGATGGCAAGTGTTTGCTCGACAATTGGTGGGATTGGGCCATATGGGTACACGAGCTCAAAGCATGGCGTGTTGGGATTGATGAAGAATGCAACGGTTGATTTAGGACGATATGGGATAAGGGTTAATTGTGTGTCACCCCATGTGGTGCCCTCGGACATAACTAGAGAGCTGTTTAAGATTGAAGATGGCAATGAGTTTCCTAATGTTTATTCGCATTTCAAATGTGGAGATATCTTAAGAGCAGAAGATATAGCTGAAACCGCTTTGTATTTAGCAAGTGATGCATCAAGGTTCGTAAGTGGACATAACTTAATTGTTGATGGAGGCTTCACTGCAGCAAACCAAGGTTTTTGCTCCTATAAACAATTTGATAACTAA

Protein sequence

MSVSLLPTAARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDLGHSLSNHLCSSSSSFVHCDVTKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESCDFQKVLNVNLLGTFFGTKHAARAMIPARQGSIIMMASVCSTIGGIGPYGYTSSKHGVLGLMKNATVDLGRYGIRVNCVSPHVVPSDITRELFKIEDGNEFPNVYSHFKCGDILRAEDIAETALYLASDASRFVSGHNLIVDGGFTAANQGFCSYKQFDN
Homology
BLAST of Csor.00g163430 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 2.5e-70
Identity = 133/260 (51.15%), Postives = 182/260 (70.00%), Query Frame = 0

Query: 5   LLPTAARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDLGHSLSNHLCSSSS 64
           +L   ARRLEGKVA+I G A GIGE+TA+ F +HGAK+ IAD+ D+LGHS+   + +S+S
Sbjct: 7   VLTAIARRLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVVEAIGTSNS 66

Query: 65  SFVHCDVTKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESCDFQKVLNVNL 124
           +++HCDVT E  V+NAV+  +S YGKLDIM +NAGI       I++NE  DF++V +VN+
Sbjct: 67  TYIHCDVTNEDGVKNAVDNTVSTYGKLDIMFSNAGISDPNRPRIIDNEKADFERVFSVNV 126

Query: 125 LGTFFGTKHAARAMIPARQGSIIMMASVCSTIGGIGPYGYTSSKHGVLGLMKNATVDLGR 184
            G F   KHAAR MIPAR G+II  AS+ ST+GG   + Y  SKH VLGL +N  V+LG+
Sbjct: 127 TGVFLCMKHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTRNLAVELGQ 186

Query: 185 YGIRVNCVSPHVVPSDITRELFKIEDGNEFPNV--YSHFKCGDILRAEDIAETALYLASD 244
           +GIRVNC+SP  +P+ + ++   I++  EF NV  ++    G     ED+A  ALYLASD
Sbjct: 187 FGIRVNCLSPFGLPTALGKKFSGIKNEEEFENVINFAGNLKGPKFNVEDVANAALYLASD 246

Query: 245 ASRFVSGHNLIVDGGFTAAN 263
            +++VSGHNL +DGGF+  N
Sbjct: 247 EAKYVSGHNLFIDGGFSVCN 266

BLAST of Csor.00g163430 vs. ExPASy Swiss-Prot
Match: Q7FAE1 (Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 PE=2 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 4.4e-67
Identity = 134/258 (51.94%), Postives = 174/258 (67.44%), Query Frame = 0

Query: 10  ARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDLGHSLSNHLCSSSSSFVHC 69
           AR+L GKVAVI G A GIG  TAR F KHGA++V+ADI D+LG SL   L   +SS+VHC
Sbjct: 12  ARKLVGKVAVITGGASGIGACTARLFVKHGARVVVADIQDELGASLVAELGPDASSYVHC 71

Query: 70  DVTKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESCDFQKVLNVNLLGTFF 129
           DVT E DV  AV+ A++++GKLD+M NNAG+ G P F + E    DF++VL VNL+G F 
Sbjct: 72  DVTNEGDVAAAVDHAVARFGKLDVMFNNAGVSGPPCFRMSECTKEDFERVLAVNLVGPFL 131

Query: 130 GTKHAARAMIPARQGSIIMMASVCSTIGGIGPYGYTSSKHGVLGLMKNATVDLGRYGIRV 189
           GTKHAAR M PAR+GSII  AS+ S++ G   + YT+SKH ++G  +NA  +LGR+GIRV
Sbjct: 132 GTKHAARVMAPARRGSIISTASLSSSVSGAASHAYTTSKHALVGFTENAAGELGRHGIRV 191

Query: 190 NCVSPHVVPSDITRELFKIEDG--NEFPNVYSHFKCGDILRAEDIAETALYLASDASRFV 249
           NCVSP  V + + R    ++D          ++ K    L+A+DIA  AL+LASD  R+V
Sbjct: 192 NCVSPAGVATPLARAAMGMDDEAIEAIMANSANLKGAGALKADDIAAAALFLASDDGRYV 251

Query: 250 SGHNLIVDGGFTAANQGF 266
           SG NL VDGG +  N  F
Sbjct: 252 SGQNLRVDGGLSVVNSSF 269

BLAST of Csor.00g163430 vs. ExPASy Swiss-Prot
Match: Q94KL8 (Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 PE=1 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 1.8e-65
Identity = 129/268 (48.13%), Postives = 183/268 (68.28%), Query Frame = 0

Query: 2   SVSLLPTAARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDLGHSLSNHLCS 61
           S S   ++  RL+ KVA+I G A GIGE+TA+ F ++GAK+VIADI DD G  + N++ S
Sbjct: 3   STSTPDSSTNRLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADIADDHGQKVCNNIGS 62

Query: 62  SS-SSFVHCDVTKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESCDFQKVL 121
               SFVHCDVTK++DV N V+T I+K+GKLDIM  N G+L    ++ILE  + DF++V+
Sbjct: 63  PDVISFVHCDVTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVM 122

Query: 122 NVNLLGTFFGTKHAARAMIPARQGSIIMMASVCSTIGGIG-PYGYTSSKHGVLGLMKNAT 181
           ++N+ G F   KHAAR MIPA++GSI+  AS+ S   G G  + YT++KH VLGL  +  
Sbjct: 123 DINVYGAFLVAKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLC 182

Query: 182 VDLGRYGIRVNCVSPHVVPSDITRELFKIEDG--NEFPNVYSHFKCGDILRAEDIAETAL 241
            +LG YGIRVNCVSP++V S +  ++F ++     E  +  ++ K G +LRAED+A+   
Sbjct: 183 TELGEYGIRVNCVSPYIVASPLLTDVFGVDSSRVEELAHQAANLK-GTLLRAEDVADAVA 242

Query: 242 YLASDASRFVSGHNLIVDGGFTAANQGF 266
           YLA D S++VSG NL++DGG+T  N  F
Sbjct: 243 YLAGDESKYVSGLNLVIDGGYTRTNPAF 269

BLAST of Csor.00g163430 vs. ExPASy Swiss-Prot
Match: A3F5F0 (Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH PE=1 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 9.1e-65
Identity = 129/268 (48.13%), Postives = 184/268 (68.66%), Query Frame = 0

Query: 2   SVSLLPTAARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDLGHSLSNHLCS 61
           S S   ++  RL+ KVA+I G A GIGE+TA+ F ++GAK+VIADI DD G  + N++ S
Sbjct: 3   STSTPASSTNRLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADISDDHGQKVCNNIGS 62

Query: 62  SS-SSFVHCDVTKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESCDFQKVL 121
               SFVHCDVTK++DV N V+T I+K+GKLDIM  N G+L    ++ILE  + DF++V+
Sbjct: 63  PDVISFVHCDVTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVM 122

Query: 122 NVNLLGTFFGTKHAARAMIPARQGSIIMMASVCSTIGGIG-PYGYTSSKHGVLGLMKNAT 181
           ++N+ G F   KHAAR MIPA++GSI+  AS+ S   G G  + YT++KH VLGL  +  
Sbjct: 123 DINVYGAFLVAKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLC 182

Query: 182 VDLGRYGIRVNCVSPHVVPSDITRELFKIEDG--NEFPNVYSHFKCGDILRAEDIAETAL 241
            +LG++GIRVNCVSP+VV S +  ++F ++     E  +  ++ K G +LRAED+A+   
Sbjct: 183 TELGQHGIRVNCVSPYVVASPLLTDVFGVDSSRVEELAHQAANLK-GILLRAEDVADAVA 242

Query: 242 YLASDASRFVSGHNLIVDGGFTAANQGF 266
           YLA D S++VSG NL++DGG+T  N  F
Sbjct: 243 YLAGDESKYVSGLNLVIDGGYTRTNPAF 269

BLAST of Csor.00g163430 vs. ExPASy Swiss-Prot
Match: F1SWA0 (Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 5.0e-63
Identity = 123/260 (47.31%), Postives = 171/260 (65.77%), Query Frame = 0

Query: 12  RLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDLGHSLSNHLCSS-SSSFVHCD 71
           RLEGKVA++ G A GIGES AR F +HGAK+ I D+ D+LG  +S  L     + + HCD
Sbjct: 2   RLEGKVALVTGGASGIGESIARLFIEHGAKICIVDVQDELGQQVSQRLGGDPHACYFHCD 61

Query: 72  VTKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESCDFQKVLNVNLLGTFFG 131
           VT E DV  AV+    KYG +DIM+NNAGI G+ + +I + +  +F+KV ++N+ G F G
Sbjct: 62  VTVEDDVRRAVDFTAEKYGTIDIMVNNAGITGDKVIDIRDADFNEFKKVFDINVNGVFLG 121

Query: 132 TKHAARAMIPARQGSIIMMASVCSTIGGIGPYGYTSSKHGVLGLMKNATVDLGRYGIRVN 191
            KHAAR MIP  +GSI+ +ASV S I G GP+GYT +KH V+GL K+   +LGR+GIRVN
Sbjct: 122 MKHAARIMIPKMKGSIVSLASVSSVIAGAGPHGYTGAKHAVVGLTKSVAAELGRHGIRVN 181

Query: 192 CVSPHVVPSDITRELFKIEDGNE--------FPNVYSHFKCGDILRAEDIAETALYLASD 251
           CVSP+ VP+ ++       +  E        F    ++ K  D++   D+AE  LYLA++
Sbjct: 182 CVSPYAVPTRLSMPYLPESEMQEDALRGFLTFVRSNANLKGVDLM-PNDVAEAVLYLATE 241

Query: 252 ASRFVSGHNLIVDGGFTAAN 263
            S++VSG NL++DGGF+ AN
Sbjct: 242 ESKYVSGLNLVIDGGFSIAN 260

BLAST of Csor.00g163430 vs. NCBI nr
Match: KAG6583704.1 (hypothetical protein SDJN03_19636, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 545 bits (1405), Expect = 1.19e-195
Identity = 273/273 (100.00%), Postives = 273/273 (100.00%), Query Frame = 0

Query: 1   MSVSLLPTAARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDLGHSLSNHLC 60
           MSVSLLPTAARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDLGHSLSNHLC
Sbjct: 1   MSVSLLPTAARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDLGHSLSNHLC 60

Query: 61  SSSSSFVHCDVTKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESCDFQKVL 120
           SSSSSFVHCDVTKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESCDFQKVL
Sbjct: 61  SSSSSFVHCDVTKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESCDFQKVL 120

Query: 121 NVNLLGTFFGTKHAARAMIPARQGSIIMMASVCSTIGGIGPYGYTSSKHGVLGLMKNATV 180
           NVNLLGTFFGTKHAARAMIPARQGSIIMMASVCSTIGGIGPYGYTSSKHGVLGLMKNATV
Sbjct: 121 NVNLLGTFFGTKHAARAMIPARQGSIIMMASVCSTIGGIGPYGYTSSKHGVLGLMKNATV 180

Query: 181 DLGRYGIRVNCVSPHVVPSDITRELFKIEDGNEFPNVYSHFKCGDILRAEDIAETALYLA 240
           DLGRYGIRVNCVSPHVVPSDITRELFKIEDGNEFPNVYSHFKCGDILRAEDIAETALYLA
Sbjct: 181 DLGRYGIRVNCVSPHVVPSDITRELFKIEDGNEFPNVYSHFKCGDILRAEDIAETALYLA 240

Query: 241 SDASRFVSGHNLIVDGGFTAANQGFCSYKQFDN 273
           SDASRFVSGHNLIVDGGFTAANQGFCSYKQFDN
Sbjct: 241 SDASRFVSGHNLIVDGGFTAANQGFCSYKQFDN 273

BLAST of Csor.00g163430 vs. NCBI nr
Match: XP_022927563.1 (secoisolariciresinol dehydrogenase-like [Cucurbita moschata])

HSP 1 Score: 523 bits (1346), Expect = 1.17e-186
Identity = 262/273 (95.97%), Postives = 267/273 (97.80%), Query Frame = 0

Query: 1   MSVSLLPTAARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDLGHSLSNHLC 60
           MSVSLLPTAARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDD+GHSLSNHLC
Sbjct: 1   MSVSLLPTAARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDVGHSLSNHLC 60

Query: 61  SSSSSFVHCDVTKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESCDFQKVL 120
           SSSSSFVHCDVTKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENES DFQKVL
Sbjct: 61  SSSSSFVHCDVTKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESSDFQKVL 120

Query: 121 NVNLLGTFFGTKHAARAMIPARQGSIIMMASVCSTIGGIGPYGYTSSKHGVLGLMKNATV 180
           NVNLLGTF GTKHAARAMIPARQGSIIM ASVCS IGGIGPYGYTSSKHGVLGLMKNATV
Sbjct: 121 NVNLLGTFLGTKHAARAMIPARQGSIIMTASVCSAIGGIGPYGYTSSKHGVLGLMKNATV 180

Query: 181 DLGRYGIRVNCVSPHVVPSDITRELFKIEDGNEFPNVYSHFKCGDILRAEDIAETALYLA 240
           DLGRYGIRVNCVSPHVVPSDITRELFK+EDG+EFPNVYSHFKCGDILRAEDIAE ALYLA
Sbjct: 181 DLGRYGIRVNCVSPHVVPSDITRELFKLEDGDEFPNVYSHFKCGDILRAEDIAEAALYLA 240

Query: 241 SDASRFVSGHNLIVDGGFTAANQGFCSYKQFDN 273
           SDASRFVSGHNLIVDGGFTAANQGFCSY+Q D+
Sbjct: 241 SDASRFVSGHNLIVDGGFTAANQGFCSYQQSDD 273

BLAST of Csor.00g163430 vs. NCBI nr
Match: XP_023520696.1 (secoisolariciresinol dehydrogenase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 521 bits (1341), Expect = 6.77e-186
Identity = 260/273 (95.24%), Postives = 267/273 (97.80%), Query Frame = 0

Query: 1   MSVSLLPTAARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDLGHSLSNHLC 60
           MSVSLLPTAARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADI DD+GHSLSNHLC
Sbjct: 1   MSVSLLPTAARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADIQDDVGHSLSNHLC 60

Query: 61  SSSSSFVHCDVTKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESCDFQKVL 120
           SSSSSFVHCDVTKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILEN+S DFQKVL
Sbjct: 61  SSSSSFVHCDVTKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENDSSDFQKVL 120

Query: 121 NVNLLGTFFGTKHAARAMIPARQGSIIMMASVCSTIGGIGPYGYTSSKHGVLGLMKNATV 180
           NVNLLGTF GTKHAARAMIPARQGSIIM ASVCS IGGIGPYGYTSSKHGVLGLMKNATV
Sbjct: 121 NVNLLGTFLGTKHAARAMIPARQGSIIMTASVCSAIGGIGPYGYTSSKHGVLGLMKNATV 180

Query: 181 DLGRYGIRVNCVSPHVVPSDITRELFKIEDGNEFPNVYSHFKCGDILRAEDIAETALYLA 240
           DLGRYGIRVNCVSPHVVPSD+T+ELFK+EDG+EFPNVYSHFKCGDILRAEDIAETALYLA
Sbjct: 181 DLGRYGIRVNCVSPHVVPSDMTKELFKLEDGDEFPNVYSHFKCGDILRAEDIAETALYLA 240

Query: 241 SDASRFVSGHNLIVDGGFTAANQGFCSYKQFDN 273
           SDASRFVSGHNLIVDGGFTAANQGFCSY+Q DN
Sbjct: 241 SDASRFVSGHNLIVDGGFTAANQGFCSYQQSDN 273

BLAST of Csor.00g163430 vs. NCBI nr
Match: XP_022973161.1 (secoisolariciresinol dehydrogenase-like [Cucurbita maxima])

HSP 1 Score: 505 bits (1301), Expect = 8.46e-180
Identity = 253/273 (92.67%), Postives = 262/273 (95.97%), Query Frame = 0

Query: 1   MSVSLLPTAARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDLGHSLSNHLC 60
           MSVSLLPTAARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDD+GHSLSNHLC
Sbjct: 1   MSVSLLPTAARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDVGHSLSNHLC 60

Query: 61  SSSSSFVHCDVTKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESCDFQKVL 120
           SSSSSFVHCDVTKEK+VENAVNTAISKYGKLDIMLNNAGILGNPIFNILENES DFQKVL
Sbjct: 61  SSSSSFVHCDVTKEKEVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESSDFQKVL 120

Query: 121 NVNLLGTFFGTKHAARAMIPARQGSIIMMASVCSTIGGIGPYGYTSSKHGVLGLMKNATV 180
           NVNLLGTF GTKHAARAMIPARQGSIIM ASVCS IGGIGPYGYTSSKHGVLGLMKNATV
Sbjct: 121 NVNLLGTFLGTKHAARAMIPARQGSIIMTASVCSVIGGIGPYGYTSSKHGVLGLMKNATV 180

Query: 181 DLGRYGIRVNCVSPHVVPSDITRELFKIEDGNEFPNVYSHFKCGDILRAEDIAETALYLA 240
           DLGRYGIRVNCVSPHVVPS +T++LFK+EDG+EFPN YSHFK GDILRA+DIAE ALYLA
Sbjct: 181 DLGRYGIRVNCVSPHVVPSQMTKDLFKLEDGDEFPNFYSHFKYGDILRADDIAEAALYLA 240

Query: 241 SDASRFVSGHNLIVDGGFTAANQGFCSYKQFDN 273
           SDASRFVSGHNLIVDGGF AANQGFCSY+  DN
Sbjct: 241 SDASRFVSGHNLIVDGGFIAANQGFCSYQPSDN 273

BLAST of Csor.00g163430 vs. NCBI nr
Match: XP_022973162.1 (secoisolariciresinol dehydrogenase-like [Cucurbita maxima])

HSP 1 Score: 481 bits (1239), Expect = 2.06e-170
Identity = 239/269 (88.85%), Postives = 251/269 (93.31%), Query Frame = 0

Query: 1   MSVSLLPTAARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDLGHSLSNHLC 60
           M VSLLP AARRLEGKVAVI GAARGIGESTA+ FFKHGAK+V+ADILDD+GHSLS HLC
Sbjct: 1   MGVSLLPAAARRLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLC 60

Query: 61  SSSSSFVHCDVTKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESCDFQKVL 120
           SSSSSFVHCDVTKEKD+ENAVNTAI+KYGKLDIMLNNAGI G   FN LENES DFQ VL
Sbjct: 61  SSSSSFVHCDVTKEKDIENAVNTAIAKYGKLDIMLNNAGISGTLKFNFLENESSDFQNVL 120

Query: 121 NVNLLGTFFGTKHAARAMIPARQGSIIMMASVCSTIGGIGPYGYTSSKHGVLGLMKNATV 180
           NVNLLGTF GTKHAARAMIPARQGSIIM ASVCS IGGIGPYGYTSSKHGVLGLMKNATV
Sbjct: 121 NVNLLGTFLGTKHAARAMIPARQGSIIMTASVCSVIGGIGPYGYTSSKHGVLGLMKNATV 180

Query: 181 DLGRYGIRVNCVSPHVVPSDITRELFKIEDGNEFPNVYSHFKCGDILRAEDIAETALYLA 240
           DLGRYGIRVNCVSPHVVPS +TR+LFK+EDG+EFPNVYSHFKCGDILRAEDIAE ALYLA
Sbjct: 181 DLGRYGIRVNCVSPHVVPSQMTRDLFKLEDGDEFPNVYSHFKCGDILRAEDIAEAALYLA 240

Query: 241 SDASRFVSGHNLIVDGGFTAANQGFCSYK 269
           SDASRFVSGHNLIVDGGFT+ANQG CSY+
Sbjct: 241 SDASRFVSGHNLIVDGGFTSANQGLCSYQ 269

BLAST of Csor.00g163430 vs. ExPASy TrEMBL
Match: A0A6J1ELC4 (secoisolariciresinol dehydrogenase-like OS=Cucurbita moschata OX=3662 GN=LOC111434357 PE=4 SV=1)

HSP 1 Score: 523 bits (1346), Expect = 5.67e-187
Identity = 262/273 (95.97%), Postives = 267/273 (97.80%), Query Frame = 0

Query: 1   MSVSLLPTAARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDLGHSLSNHLC 60
           MSVSLLPTAARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDD+GHSLSNHLC
Sbjct: 1   MSVSLLPTAARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDVGHSLSNHLC 60

Query: 61  SSSSSFVHCDVTKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESCDFQKVL 120
           SSSSSFVHCDVTKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENES DFQKVL
Sbjct: 61  SSSSSFVHCDVTKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESSDFQKVL 120

Query: 121 NVNLLGTFFGTKHAARAMIPARQGSIIMMASVCSTIGGIGPYGYTSSKHGVLGLMKNATV 180
           NVNLLGTF GTKHAARAMIPARQGSIIM ASVCS IGGIGPYGYTSSKHGVLGLMKNATV
Sbjct: 121 NVNLLGTFLGTKHAARAMIPARQGSIIMTASVCSAIGGIGPYGYTSSKHGVLGLMKNATV 180

Query: 181 DLGRYGIRVNCVSPHVVPSDITRELFKIEDGNEFPNVYSHFKCGDILRAEDIAETALYLA 240
           DLGRYGIRVNCVSPHVVPSDITRELFK+EDG+EFPNVYSHFKCGDILRAEDIAE ALYLA
Sbjct: 181 DLGRYGIRVNCVSPHVVPSDITRELFKLEDGDEFPNVYSHFKCGDILRAEDIAEAALYLA 240

Query: 241 SDASRFVSGHNLIVDGGFTAANQGFCSYKQFDN 273
           SDASRFVSGHNLIVDGGFTAANQGFCSY+Q D+
Sbjct: 241 SDASRFVSGHNLIVDGGFTAANQGFCSYQQSDD 273

BLAST of Csor.00g163430 vs. ExPASy TrEMBL
Match: A0A6J1I7X4 (secoisolariciresinol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111471669 PE=4 SV=1)

HSP 1 Score: 505 bits (1301), Expect = 4.10e-180
Identity = 253/273 (92.67%), Postives = 262/273 (95.97%), Query Frame = 0

Query: 1   MSVSLLPTAARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDLGHSLSNHLC 60
           MSVSLLPTAARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDD+GHSLSNHLC
Sbjct: 1   MSVSLLPTAARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDVGHSLSNHLC 60

Query: 61  SSSSSFVHCDVTKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESCDFQKVL 120
           SSSSSFVHCDVTKEK+VENAVNTAISKYGKLDIMLNNAGILGNPIFNILENES DFQKVL
Sbjct: 61  SSSSSFVHCDVTKEKEVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESSDFQKVL 120

Query: 121 NVNLLGTFFGTKHAARAMIPARQGSIIMMASVCSTIGGIGPYGYTSSKHGVLGLMKNATV 180
           NVNLLGTF GTKHAARAMIPARQGSIIM ASVCS IGGIGPYGYTSSKHGVLGLMKNATV
Sbjct: 121 NVNLLGTFLGTKHAARAMIPARQGSIIMTASVCSVIGGIGPYGYTSSKHGVLGLMKNATV 180

Query: 181 DLGRYGIRVNCVSPHVVPSDITRELFKIEDGNEFPNVYSHFKCGDILRAEDIAETALYLA 240
           DLGRYGIRVNCVSPHVVPS +T++LFK+EDG+EFPN YSHFK GDILRA+DIAE ALYLA
Sbjct: 181 DLGRYGIRVNCVSPHVVPSQMTKDLFKLEDGDEFPNFYSHFKYGDILRADDIAEAALYLA 240

Query: 241 SDASRFVSGHNLIVDGGFTAANQGFCSYKQFDN 273
           SDASRFVSGHNLIVDGGF AANQGFCSY+  DN
Sbjct: 241 SDASRFVSGHNLIVDGGFIAANQGFCSYQPSDN 273

BLAST of Csor.00g163430 vs. ExPASy TrEMBL
Match: A0A6J1I6S5 (secoisolariciresinol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111471670 PE=4 SV=1)

HSP 1 Score: 481 bits (1239), Expect = 9.95e-171
Identity = 239/269 (88.85%), Postives = 251/269 (93.31%), Query Frame = 0

Query: 1   MSVSLLPTAARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDLGHSLSNHLC 60
           M VSLLP AARRLEGKVAVI GAARGIGESTA+ FFKHGAK+V+ADILDD+GHSLS HLC
Sbjct: 1   MGVSLLPAAARRLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLC 60

Query: 61  SSSSSFVHCDVTKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESCDFQKVL 120
           SSSSSFVHCDVTKEKD+ENAVNTAI+KYGKLDIMLNNAGI G   FN LENES DFQ VL
Sbjct: 61  SSSSSFVHCDVTKEKDIENAVNTAIAKYGKLDIMLNNAGISGTLKFNFLENESSDFQNVL 120

Query: 121 NVNLLGTFFGTKHAARAMIPARQGSIIMMASVCSTIGGIGPYGYTSSKHGVLGLMKNATV 180
           NVNLLGTF GTKHAARAMIPARQGSIIM ASVCS IGGIGPYGYTSSKHGVLGLMKNATV
Sbjct: 121 NVNLLGTFLGTKHAARAMIPARQGSIIMTASVCSVIGGIGPYGYTSSKHGVLGLMKNATV 180

Query: 181 DLGRYGIRVNCVSPHVVPSDITRELFKIEDGNEFPNVYSHFKCGDILRAEDIAETALYLA 240
           DLGRYGIRVNCVSPHVVPS +TR+LFK+EDG+EFPNVYSHFKCGDILRAEDIAE ALYLA
Sbjct: 181 DLGRYGIRVNCVSPHVVPSQMTRDLFKLEDGDEFPNVYSHFKCGDILRAEDIAEAALYLA 240

Query: 241 SDASRFVSGHNLIVDGGFTAANQGFCSYK 269
           SDASRFVSGHNLIVDGGFT+ANQG CSY+
Sbjct: 241 SDASRFVSGHNLIVDGGFTSANQGLCSYQ 269

BLAST of Csor.00g163430 vs. ExPASy TrEMBL
Match: A0A6J1EID0 (secoisolariciresinol dehydrogenase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434358 PE=4 SV=1)

HSP 1 Score: 473 bits (1216), Expect = 3.18e-167
Identity = 234/269 (86.99%), Postives = 249/269 (92.57%), Query Frame = 0

Query: 1   MSVSLLPTAARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDLGHSLSNHLC 60
           M +SLLP AARRLEGKVAVI GAARGIGESTA+ FFKHGAK+V+ADILDD+GHSLS HLC
Sbjct: 1   MGISLLPVAARRLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLC 60

Query: 61  SSSSSFVHCDVTKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESCDFQKVL 120
           SSSSSFVHCDVTKEKD+ENAVNTAISKYGKLDIMLNNAGI G   FNILENE  DFQKVL
Sbjct: 61  SSSSSFVHCDVTKEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVL 120

Query: 121 NVNLLGTFFGTKHAARAMIPARQGSIIMMASVCSTIGGIGPYGYTSSKHGVLGLMKNATV 180
           NVNLLG F GTKHAA+AMIPARQGSII+MAS CS IGGIGPY YTSSKHG+LGLMKNATV
Sbjct: 121 NVNLLGAFLGTKHAAKAMIPARQGSIIIMASGCSIIGGIGPYAYTSSKHGLLGLMKNATV 180

Query: 181 DLGRYGIRVNCVSPHVVPSDITRELFKIEDGNEFPNVYSHFKCGDILRAEDIAETALYLA 240
           DLGRYGIRVNCVSPHVVPS +TR+LFK+EDG+EFPNVYSHFKCGDILR EDIAE ALYLA
Sbjct: 181 DLGRYGIRVNCVSPHVVPSQMTRDLFKLEDGDEFPNVYSHFKCGDILRPEDIAEAALYLA 240

Query: 241 SDASRFVSGHNLIVDGGFTAANQGFCSYK 269
           SDASRFVSGHNLIVDGGFT+ANQG CSY+
Sbjct: 241 SDASRFVSGHNLIVDGGFTSANQGLCSYE 269

BLAST of Csor.00g163430 vs. ExPASy TrEMBL
Match: A0A6J1EI14 (secoisolariciresinol dehydrogenase-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111434358 PE=4 SV=1)

HSP 1 Score: 473 bits (1216), Expect = 3.18e-167
Identity = 234/269 (86.99%), Postives = 249/269 (92.57%), Query Frame = 0

Query: 1   MSVSLLPTAARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDLGHSLSNHLC 60
           M +SLLP AARRLEGKVAVI GAARGIGESTA+ FFKHGAK+V+ADILDD+GHSLS HLC
Sbjct: 1   MGISLLPAAARRLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLC 60

Query: 61  SSSSSFVHCDVTKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESCDFQKVL 120
           SSSSSFVHCDVTKEKD+ENAVNTAISKYGKLDIMLNNAGI G   FNILENE  DFQKVL
Sbjct: 61  SSSSSFVHCDVTKEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVL 120

Query: 121 NVNLLGTFFGTKHAARAMIPARQGSIIMMASVCSTIGGIGPYGYTSSKHGVLGLMKNATV 180
           NVNLLG F GTKHAA+AMIPARQGSII+MAS CS IGGIGPY YTSSKHG+LGLMKNATV
Sbjct: 121 NVNLLGAFLGTKHAAKAMIPARQGSIIIMASGCSIIGGIGPYAYTSSKHGLLGLMKNATV 180

Query: 181 DLGRYGIRVNCVSPHVVPSDITRELFKIEDGNEFPNVYSHFKCGDILRAEDIAETALYLA 240
           DLGRYGIRVNCVSPHVVPS +TR+LFK+EDG+EFPNVYSHFKCGDILR EDIAE ALYLA
Sbjct: 181 DLGRYGIRVNCVSPHVVPSQMTRDLFKLEDGDEFPNVYSHFKCGDILRPEDIAEAALYLA 240

Query: 241 SDASRFVSGHNLIVDGGFTAANQGFCSYK 269
           SDASRFVSGHNLIVDGGFT+ANQG CSY+
Sbjct: 241 SDASRFVSGHNLIVDGGFTSANQGLCSYE 269

BLAST of Csor.00g163430 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 237.3 bits (604), Expect = 1.5e-62
Identity = 128/269 (47.58%), Postives = 177/269 (65.80%), Query Frame = 0

Query: 11  RRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILD----DLGHSLSNHLCSSSSSF 70
           +RLEGKVA+I G A GIG++T   F +HGA +VIAD+ +     L  SLS+H  S   +F
Sbjct: 30  KRLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKSLSSHKTSPMVAF 89

Query: 71  VHCDVTKEKDVENAVNTAISKYGKLDIMLNNAGILGN--PIFNILENESCDFQKVLNVNL 130
           + CDV+ E DVEN VN  +++YG+LDI+ NNAG+LG+     +IL+ ++ +F  V+ VN+
Sbjct: 90  ISCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFDADEFDHVMRVNV 149

Query: 131 LGTFFGTKHAARAMIP-ARQGSIIMMASVCSTIGGIGPYGYTSSKHGVLGLMKNATVDLG 190
            G   G KH ARAMI    +G II  ASV   +GG+GP+ YT+SKH ++GL KNA  +LG
Sbjct: 150 RGVGLGMKHGARAMIKRGFKGCIISTASVAGVMGGMGPHAYTASKHAIVGLTKNAACELG 209

Query: 191 RYGIRVNCVSPHVVPSDITRELFK-----------IEDGNEFPNVYSHFKCGDILRAEDI 250
           +YGIRVNC+SP  V + +    ++           +E+  EF    ++ K G+ LRA DI
Sbjct: 210 KYGIRVNCISPFGVATSMLVNAWRKTSGGDVEDDDVEEMEEFVRSLANLK-GETLRANDI 269

Query: 251 AETALYLASDASRFVSGHNLIVDGGFTAA 262
           AE ALYLASD S++V+GHNL+VDGG T A
Sbjct: 270 AEAALYLASDESKYVNGHNLVVDGGVTTA 297

BLAST of Csor.00g163430 vs. TAIR 10
Match: AT1G52340.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 227.3 bits (578), Expect = 1.5e-59
Identity = 122/276 (44.20%), Postives = 166/276 (60.14%), Query Frame = 0

Query: 2   SVSLLPTAARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDLGHSLSNHLCS 61
           S S LP  ++RL GKVA+I G A GIGES  R F KHGAK+ I D+ DDLG  +   L  
Sbjct: 9   SYSSLP--SQRLLGKVALITGGATGIGESIVRLFHKHGAKVCIVDLQDDLGGEVCKSLLR 68

Query: 62  SSSS----FVHCDVTKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESCDFQ 121
             S     F+H DV  E D+ NAV+ A+  +G LDI++NNAG+ G P  +I      +F+
Sbjct: 69  GESKETAFFIHGDVRVEDDISNAVDFAVKNFGTLDILINNAGLCGAPCPDIRNYSLSEFE 128

Query: 122 KVLNVNLLGTFFGTKHAARAMIPARQGSIIMMASVCSTIGGIGPYGYTSSKHGVLGLMKN 181
              +VN+ G F   KHAAR MIP ++GSI+ + SV   +GG+GP+ Y  SKH VLGL ++
Sbjct: 129 MTFDVNVKGAFLSMKHAARVMIPEKKGSIVSLCSVGGVVGGVGPHSYVGSKHAVLGLTRS 188

Query: 182 ATVDLGRYGIRVNCVSPHVVPSDITRELFKIEDGNE--------FPNVYSHFKCGDILRA 241
              +LG++GIRVNCVSP+ V + +       E+  E        F    ++ K G  L  
Sbjct: 189 VAAELGQHGIRVNCVSPYAVATKLALAHLPEEERTEDAFVGFRNFAAANANLK-GVELTV 248

Query: 242 EDIAETALYLASDASRFVSGHNLIVDGGFTAANQGF 266
           +D+A   L+LASD SR++SG NL++DGGFT  N  F
Sbjct: 249 DDVANAVLFLASDDSRYISGDNLMIDGGFTCTNHSF 281

BLAST of Csor.00g163430 vs. TAIR 10
Match: AT3G26760.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 219.2 bits (557), Expect = 4.2e-57
Identity = 116/270 (42.96%), Postives = 176/270 (65.19%), Query Frame = 0

Query: 2   SVSLLPTAARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDLGHSLSNHLCS 61
           S  L  T++R+LEGKVAVI G A GIG++TA  F   GA+++I DI ++ GH ++  L  
Sbjct: 25  STLLYSTSSRKLEGKVAVITGGASGIGKATAEEFVSQGAQVIIVDIDEEAGHMVATEL-G 84

Query: 62  SSSSFVHCDVTKEKDVENAVNTAISKYGKLDIMLNNAGI---LGNPIFNILENESCDFQK 121
           S++ F+ CDVT+E+ +  AV TA++++GKLD+MLN+AGI   +  P    L+ ++ D  K
Sbjct: 85  SAAHFLRCDVTEEEQIAKAVETAVTRHGKLDVMLNSAGISCSISPPSIADLDMDTYD--K 144

Query: 122 VLNVNLLGTFFGTKHAARAMIPARQGSIIMMASVCSTIGGIGPYGYTSSKHGVLGLMKNA 181
           V+ +N+ GT  G KHAARAMIPA  GSI+ ++S+   +GG+GP+ Y+ SK  + G++K  
Sbjct: 145 VMRLNVRGTVLGIKHAARAMIPAGSGSILCLSSISGLMGGLGPHAYSISKFTIPGVVKTV 204

Query: 182 TVDLGRYGIRVNCVSPHVVPSDITRELF---------KIEDGNEFPNVYSHFKCGDILRA 241
             +L ++G+R+NC+SP  +P+ +T  +F         + E      N     K G+    
Sbjct: 205 ASELCKHGLRINCISPAGIPTPLTLRMFREAFAGHSIREEQLLAIVNATGELK-GEKCEE 264

Query: 242 EDIAETALYLASDASRFVSGHNLIVDGGFT 260
            D+A+ ALYLASD ++FV+GHNL+VDGGFT
Sbjct: 265 IDVAKAALYLASDDAKFVTGHNLVVDGGFT 290

BLAST of Csor.00g163430 vs. TAIR 10
Match: AT2G47140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 217.2 bits (552), Expect = 1.6e-56
Identity = 112/253 (44.27%), Postives = 165/253 (65.22%), Query Frame = 0

Query: 9   AARRLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDLGHSLSNHLCSSSSSFVH 68
           + +RL+GK+ +I G A GIG  + R F +HGA++VI D+ D+LG +++  +    +S+ H
Sbjct: 2   SGKRLDGKIVIITGGASGIGAESVRLFTEHGARVVIVDVQDELGQNVAVSIGEDKASYYH 61

Query: 69  CDVTKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESCDFQKVLNVNLLGTF 128
           CDVT E +VENAV   + KYGKLD++ +NAG++  P  +IL+    +  + + +NL GT 
Sbjct: 62  CDVTNETEVENAVKFTVEKYGKLDVLFSNAGVI-EPFVSILDLNLNELDRTIAINLRGTA 121

Query: 129 FGTKHAARAMI-PARQGSIIMMASVCSTIGGIGPYGYTSSKHGVLGLMKNATVDLGRYGI 188
              KHAARAM+    +GSI+   SV + I G  P+GYT+SKHG+LGL+K+A+  LG+YGI
Sbjct: 122 AFIKHAARAMVEKGIRGSIVCTTSVAAEIAGTAPHGYTTSKHGLLGLIKSASGGLGKYGI 181

Query: 189 RVNCVSPHVVPSDITRELFKIEDGNEFPNVYSHFKC-GDILRAEDIAETALYLASDASRF 248
           RVN V+P  V + +    FK+E      N  +     G +L+A  +AE AL+LASD S +
Sbjct: 182 RVNGVAPFGVATPLVCNGFKMEPNVVEQNTSASANLKGIVLKARHVAEAALFLASDESAY 241

Query: 249 VSGHNLIVDGGFT 260
           VSG NL VDGG++
Sbjct: 242 VSGQNLAVDGGYS 253

BLAST of Csor.00g163430 vs. TAIR 10
Match: AT2G47130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 209.5 bits (532), Expect = 3.3e-54
Identity = 114/250 (45.60%), Postives = 160/250 (64.00%), Query Frame = 0

Query: 12  RLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDLGHSLSNHLCSSSSSFVHCDV 71
           RL+GK+A+I G A GIG    R F  HGAK+VI D  ++LG +++  +    +SF  CDV
Sbjct: 5   RLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDFQEELGQNVAVSVGKDKASFYRCDV 64

Query: 72  TKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESCDFQKVLNVNLLGTFFGT 131
           T EK+VENAV   + KYGKLD++ +NAG++  P  + L+     F + + VN+ G     
Sbjct: 65  TNEKEVENAVKFTVEKYGKLDVLFSNAGVMEQP-GSFLDLNLEQFDRTMAVNVRGAAAFI 124

Query: 132 KHAARAMI-PARQGSIIMMASVCSTIGGIGPYGYTSSKHGVLGLMKNATVDLGRYGIRVN 191
           KHAARAM+    +GSI+   SV S IGG GP+ YT+SKH +LGL+K+A   LG+YGIRVN
Sbjct: 125 KHAARAMVEKGTRGSIVCTTSVASEIGGPGPHAYTASKHALLGLVKSACGGLGKYGIRVN 184

Query: 192 CVSPHVVPSDI-TRELFKIEDGNEFPNVYSHFKCGDILRAEDIAETALYLASDASRFVSG 251
            V+P+ V + I +R+   +    E+       K G +L+A  +AE AL+LASD S +VSG
Sbjct: 185 GVAPYAVATAINSRDEETVRMVEEYSAATGILK-GVVLKARHVAEAALFLASDDSAYVSG 244

Query: 252 HNLIVDGGFT 260
            NL VDGG++
Sbjct: 245 QNLAVDGGYS 252

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94KL72.5e-7051.15Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P... [more]
Q7FAE14.4e-6751.94Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 ... [more]
Q94KL81.8e-6548.13Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 P... [more]
A3F5F09.1e-6548.13Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH ... [more]
F1SWA05.0e-6347.31Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAG6583704.11.19e-195100.00hypothetical protein SDJN03_19636, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022927563.11.17e-18695.97secoisolariciresinol dehydrogenase-like [Cucurbita moschata][more]
XP_023520696.16.77e-18695.24secoisolariciresinol dehydrogenase-like [Cucurbita pepo subsp. pepo][more]
XP_022973161.18.46e-18092.67secoisolariciresinol dehydrogenase-like [Cucurbita maxima][more]
XP_022973162.12.06e-17088.85secoisolariciresinol dehydrogenase-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1ELC45.67e-18795.97secoisolariciresinol dehydrogenase-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1I7X44.10e-18092.67secoisolariciresinol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111471... [more]
A0A6J1I6S59.95e-17188.85secoisolariciresinol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111471... [more]
A0A6J1EID03.18e-16786.99secoisolariciresinol dehydrogenase-like isoform X1 OS=Cucurbita moschata OX=3662... [more]
A0A6J1EI143.18e-16786.99secoisolariciresinol dehydrogenase-like isoform X3 OS=Cucurbita moschata OX=3662... [more]
Match NameE-valueIdentityDescription
AT3G51680.11.5e-6247.58NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G52340.11.5e-5944.20NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G26760.14.2e-5742.96NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G47140.11.6e-5644.27NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G47130.13.3e-5445.60NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 89..100
score: 50.95
coord: 144..152
score: 46.12
coord: 164..183
score: 28.01
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 89..100
score: 57.66
coord: 222..242
score: 30.48
coord: 17..34
score: 42.16
coord: 164..183
score: 32.89
coord: 138..154
score: 30.9
coord: 185..202
score: 37.51
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 22..259
e-value: 2.2E-61
score: 207.4
NoneNo IPR availableGENE3D3.40.50.720coord: 4..271
e-value: 2.1E-71
score: 242.1
NoneNo IPR availablePIRSRPIRSR000094-3PIRSR000094-3coord: 13..260
e-value: 2.9E-15
score: 54.0
NoneNo IPR availablePANTHERPTHR43180:SF30MOMILACTONE A SYNTHASEcoord: 8..265
NoneNo IPR availablePANTHERPTHR431803-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)coord: 8..265
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 14..260

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g163430.m01Csor.00g163430.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity