Csor.00g159870 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g159870
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionCysteine protease
LocationCsor_Chr15: 11186249 .. 11190480 (+)
RNA-Seq ExpressionCsor.00g159870
SyntenyCsor.00g159870
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAAGGGGGAAAGATCTGAATTCAACATGCTCTTCTGCAAGTACAACTGATAGTATAGATAGAACTCAGAAATCTGTTTGTCCAGAGTTAGGGTCTAAAGATCACGCATCCTCCAAAGCCTCCTTATGGTCAAGCTTCCTTACATCTACTTTAACAATCTTTGAACATCACAAAGAGCCCTCTGTTAGTGAAAAGAAGACATTTCATTCTCGACAAAATGTCTGGACAACTATAAGAAAAGTTATGACTAGTGGCTCAATGAGGAGACTTCAGGAGCGCATATTGGGTTCTCACAGGAGTGGTGTCTATAGCTCCGGCGGTGATATATGGCTTCTTGGTGTGTGCCATAAAATTTCCCAGGATCAGGCTCCTGATGATGTTACTAGCAATGGTGCAGCAGGATTCGAGCTAGATTTTTCATCTAGAATTCTGATTACCTATCGTAAAGGTTTCATGAAATATGCTCTCTTTTAGTTATACTTGTATCATAAGGCATATGTTTCCTGGACAACTTGCAGTTAACTTTTTGACTCTCCATTTATAGGTTTCAATGTTATTCAAGACTCAAAATACACCAGTGATGTAAATTGGGGCTGCATGCTAAGAAGCAGTCAAATGCTTGTTGCTCAGGTAGATAAATTCATACTCTAGCCATTGCTGTTCTCACTCTCGGTTCTGATTTTTTTTTTTTTTTTTTATTTATGCATTTGTACTAACCATGCTTTGGTTATGAGGGTTTCCAAAAAGGTATTTGTTATTTCCTTTTATTAATCTCCGTATTATTTACAGGCATTACTTTTTCATAGATTAGGAAGATCTTGGAGAAAGACTTCTCGGAAGGTATGGCTGTATGGTGACGATACTGAAAGATTTGTTTGACTTACTGATGTATTTCCCAAACAATTAGCATTAAAAGTAATTGTCTTTAATTGGCAGCCATTGGATAAAGAATATGTTGAAATTTTGCACCTTTTTGGTGACTCGGAGAAGTCAGCATTTTCAATCCATAATATTCTTCAGGCAGGAAGGGCCTATGACCTTGCTGCTGGGTCATGGGTGGGACCTTATGCGATGTGTAGGTCATGGGAGACTCTTGTCCGCTTAAAGAGGGAGACCCCTAATCTCCAAGACCAGCAACTTCCAATGGCCATTTACATTGTTTCTGGAGACGAGGACGGAGAGAGAGGTGGTGCTCCAGTTTTATACATTGACGATGCCTCAAGACATTGTTTTGAGTTTTCAAAAGGCCAACTTGATTGGACCCCCATTCTTTTATTAGTTCCTTTGGTTCTTGGACTTGAAAAAATCAATTCAAGGTATGTTACAGTTGTTTACTGGCTTGTATTTTTTTTTTTTTTTTTTTTTTCGTACTTTGGGCTTAACTCTCATTTTAATAACAGCGAAATGGGATTCTCATACGAGAAGTAGTACTTTTATAGACTATAGTTTTCCCCTAATAATTGGTTTTGCATCTTCATGCTTGAGCAGTTTTATTGAAAATTAAAAGTTTCGGTAAGAAGTTGCAAATTTTGGCACCCTAGGTTTAGAATTGTTGCTGTATTAAACTCTTTCAAGTCTAGCATTTCTTCATGGTGTGAGGATTTGTGGGGAAGGGTGGGGGAAGGTTGCACTTAATGTCTATTGTTAAATTGGATTTCTTAAAAAGAAAGTTGGAAAAACGAAATGTGAAGATGTCAGGTTGGAGGATTTGTTGCTTATGGATGAGGCTAATTGGAGGATAAAATCAAACTTTAAATGGGAAATGTAGCTCTGATACTACATTTGTAGGAAGTGTGGCTTTAACTAGGAACCTTCTCATTTCCTTGAACTAGGAAATGAGCAGGCCCTAGGAACCTGCTGATTGCCTTGGGATCCTTTCTCATACTAACCAAAGGTCTCTTTATGTTCAATTTATAAGCCATGATAACAAAAATGCCGATGGCTATTTACTTCATTGCCCCAGGCCCTAGAAACCATTACTTGATTGGCTTATCAATTTCCGAATACCGTTAGTCTTAGTAGAGATCTTGAAATCTCTATTCATGTTGTAATCGTTTACTGATTGTCATAAAGAGGTACATGTATGTTTACTAGCTTGTTTGCTAAAACATAGCAGCCTCACTTGACTATTCTGGAGACCTACATGTTATTTCTGTAGTTTGACTAGTAGAAATTGGAGCTTAGAAATTTATGCTCAACATATTTATTCAACTTGTGAAGGTACATCCTATCATTGAAAACGACATTTACCTTTCCTCAAAGCCTCGGGATCTTGGGAGGGAAGCCCAGTGTTTCAACATACATTGTGGGTGTTCAAGATGAAAATGCCTTTTATCTTGATCCACATGAAGTTCAGCAGGTACATTAATTTCCTTGGAGATCCTTTTTTAAGAATATCTAAATTATCTTTTCTTTTGTATGTGTCTGACGTGTGATATCATTATGGTGATCGTAATAGATAGGTGGTGTTTCATTGGGGGATTCCATGCGAGTGAAAGAAGTTAAGCCTCTTGTTGTGAGGGGGGAACAAAAGAGGTTTGTGTTGAAAGTGAGCTAGTTTGGAAAGGAGTGGAGTTCAAAGAGTTAGTAAGGCCAAGCTCTCAAAAATTACGTTGAAGTTGGGGGTAGTGGTGTGGGTAAGCTATTGCCTTGAGAGTCTTGTCCAAAGATCGAGTGACCTTTTCTTCGGAAGATGATGAGCACCTGAGGCATTGTGTTGGGTACAAGTGGTTTTGAATGTGAGAGGAACTTGGTTTGTTGTTGCAATGGAATAGTGCAAAGGACGAAGATTATCTCTTTTTGTACCACAAGGTAGAGGGGCATGGGATTCTTTTTGAGATGCGCCAAATATCATTCTCCTAAAAGCGCTTGGTTTAACTAAGATTGAAAGAGTCGAGCCTCCTCCAAGATTAGAGTCATCCTTTGTGAAGGTGCTTCAAAGATGGCTTAACGTGTCAGAAGTGGGGGTTCAAGACGATGAGAATGAGAAGGAGCTGGCACTTAATATCGCTACGAGTGTTTTGATGATGAAGGAGAAGGCGACTGATTCTAGGGAGGAAATTGATAGGGGAGTGATGAAGTTATTTGGTGTAGACTTAAGTGCTTAAGGGGTTTGACTTGAGATGTTAGATGAAAGAGTCGAGCCTCCTCCAAAATTAGAGTCATCCTTCGTGAAGGTGCTCAAAGATGGCTTAACGTGTCAGAAGTGGGGGTTCAAGACGATGAGAATGAGAAGGAGCTGCAAGTTATTTGGTGAAGACTTAAGTGCATAAGGGGTTTGCAGGACTTTGCCAGTAATCCTATGTGAAGAGGCTCTTCTAAGGAAGGAATTGTTTTGACTTTAGATGTGGTATGGTTCTAGATTGCGCTATTAGTATCCAGCCATGGCACGAAGTGTTGGTGGCCGAAACTCACCGATTCGTGTTAGAGAAAGGGTGGGTTAGAGTATTAGATGTCCCTTAGCTCGAGAGGAACAATGGGATTTTTAAAGGGTTGAGAGTTCGTGTGATGAGGTTTGGGAGGTGGTGAGATTGAATGCTCCATTGTAGGCATGTGGACTCAGCTAATAAATTGATAAAATGTAATGAATACTATAATGTCTTGTCCAAACAAACGTATCCATGTCTCTCTCCAGGATAATTTAATGTGACATATTTGTAAATAAATCTAAGTTATCATAATGACTGTCTAACAATGTTTCCATTTTAGGTAGTTAATATTGATAAGGATGACCTAGACGCAGATACTTCCTCTTATCACTGCAAGTGAGTTTTTCCATATCATAAATGTCTCGTGGTTTACTGTTTTTCGCTTGCCAATTCTTTTTGTTTCTCATATGTTAATGCATTGTTTTCTGTAGTGTTATCCGGCACATCCCCTTAGAATCCATGGACCCTTCTCTAGCAATTGGATTTTATTGTCGAGACAAAGGTTTGGACTCTAGCTTCATAATTGTGGATAACAACTTGATTGATGCTTCCTGATTCTTTTGAAATTTTGTTCTGGTGCCAGATGATTTCGACGATTTCTGTTATCGGGCATCGAAGTTGGCAGACGAGTCGGATGGAGCGCCATTGTTTACAGTTGCTGAAACACATTCCACGAATTCAGGGAGACACGGCCATGCATTGGATAACTGTAGTAGGTTAGTGGAGGACGATAGTGATGGCGTGGTGCACATGCCGAAGGAAGAGGAGGAAGCACATGAGGACGACTGGCAATTTCTTTGA

mRNA sequence

ATGGGAAGGGGGAAAGATCTGAATTCAACATGCTCTTCTGCAAGTACAACTGATAGTATAGATAGAACTCAGAAATCTGTTTGTCCAGAGTTAGGGTCTAAAGATCACGCATCCTCCAAAGCCTCCTTATGGTCAAGCTTCCTTACATCTACTTTAACAATCTTTGAACATCACAAAGAGCCCTCTGTTAGTGAAAAGAAGACATTTCATTCTCGACAAAATGTCTGGACAACTATAAGAAAAGTTATGACTAGTGGCTCAATGAGGAGACTTCAGGAGCGCATATTGGGTTCTCACAGGAGTGGTGTCTATAGCTCCGGCGGTGATATATGGCTTCTTGGTGTGTGCCATAAAATTTCCCAGGATCAGGCTCCTGATGATGTTACTAGCAATGGTGCAGCAGGATTCGAGCTAGATTTTTCATCTAGAATTCTGATTACCTATCGTAAAGGTTTCAATGTTATTCAAGACTCAAAATACACCAGTGATGTAAATTGGGGCTGCATGCTAAGAAGCAGTCAAATGCTTGTTGCTCAGGCATTACTTTTTCATAGATTAGGAAGATCTTGGAGAAAGACTTCTCGGAAGCCATTGGATAAAGAATATGTTGAAATTTTGCACCTTTTTGGTGACTCGGAGAAGTCAGCATTTTCAATCCATAATATTCTTCAGGCAGGAAGGGCCTATGACCTTGCTGCTGGGTCATGGGTGGGACCTTATGCGATGTGTAGGTCATGGGAGACTCTTGTCCGCTTAAAGAGGGAGACCCCTAATCTCCAAGACCAGCAACTTCCAATGGCCATTTACATTGTTTCTGGAGACGAGGACGGAGAGAGAGGTGGTGCTCCAGTTTTATACATTGACGATGCCTCAAGACATTGTTTTGAGTTTTCAAAAGGCCAACTTGATTGGACCCCCATTCTTTTATTAGTTCCTTTGGTTCTTGGACTTGAAAAAATCAATTCAAGGTACATCCTATCATTGAAAACGACATTTACCTTTCCTCAAAGCCTCGGGATCTTGGGAGGGAAGCCCAGTGTTTCAACATACATTGTGGGTGTTCAAGATGAAAATGCCTTTTATCTTGATCCACATGAAGTTCAGCAGGTAGTTAATATTGATAAGGATGACCTAGACGCAGATACTTCCTCTTATCACTGCAATGTTATCCGGCACATCCCCTTAGAATCCATGGACCCTTCTCTAGCAATTGGATTTTATTGTCGAGACAAAGATGATTTCGACGATTTCTGTTATCGGGCATCGAAGTTGGCAGACGAGTCGGATGGAGCGCCATTGTTTACAGTTGCTGAAACACATTCCACGAATTCAGGGAGACACGGCCATGCATTGGATAACTGTAGTAGGTTAGTGGAGGACGATAGTGATGGCGTGGTGCACATGCCGAAGGAAGAGGAGGAAGCACATGAGGACGACTGGCAATTTCTTTGA

Coding sequence (CDS)

ATGGGAAGGGGGAAAGATCTGAATTCAACATGCTCTTCTGCAAGTACAACTGATAGTATAGATAGAACTCAGAAATCTGTTTGTCCAGAGTTAGGGTCTAAAGATCACGCATCCTCCAAAGCCTCCTTATGGTCAAGCTTCCTTACATCTACTTTAACAATCTTTGAACATCACAAAGAGCCCTCTGTTAGTGAAAAGAAGACATTTCATTCTCGACAAAATGTCTGGACAACTATAAGAAAAGTTATGACTAGTGGCTCAATGAGGAGACTTCAGGAGCGCATATTGGGTTCTCACAGGAGTGGTGTCTATAGCTCCGGCGGTGATATATGGCTTCTTGGTGTGTGCCATAAAATTTCCCAGGATCAGGCTCCTGATGATGTTACTAGCAATGGTGCAGCAGGATTCGAGCTAGATTTTTCATCTAGAATTCTGATTACCTATCGTAAAGGTTTCAATGTTATTCAAGACTCAAAATACACCAGTGATGTAAATTGGGGCTGCATGCTAAGAAGCAGTCAAATGCTTGTTGCTCAGGCATTACTTTTTCATAGATTAGGAAGATCTTGGAGAAAGACTTCTCGGAAGCCATTGGATAAAGAATATGTTGAAATTTTGCACCTTTTTGGTGACTCGGAGAAGTCAGCATTTTCAATCCATAATATTCTTCAGGCAGGAAGGGCCTATGACCTTGCTGCTGGGTCATGGGTGGGACCTTATGCGATGTGTAGGTCATGGGAGACTCTTGTCCGCTTAAAGAGGGAGACCCCTAATCTCCAAGACCAGCAACTTCCAATGGCCATTTACATTGTTTCTGGAGACGAGGACGGAGAGAGAGGTGGTGCTCCAGTTTTATACATTGACGATGCCTCAAGACATTGTTTTGAGTTTTCAAAAGGCCAACTTGATTGGACCCCCATTCTTTTATTAGTTCCTTTGGTTCTTGGACTTGAAAAAATCAATTCAAGGTACATCCTATCATTGAAAACGACATTTACCTTTCCTCAAAGCCTCGGGATCTTGGGAGGGAAGCCCAGTGTTTCAACATACATTGTGGGTGTTCAAGATGAAAATGCCTTTTATCTTGATCCACATGAAGTTCAGCAGGTAGTTAATATTGATAAGGATGACCTAGACGCAGATACTTCCTCTTATCACTGCAATGTTATCCGGCACATCCCCTTAGAATCCATGGACCCTTCTCTAGCAATTGGATTTTATTGTCGAGACAAAGATGATTTCGACGATTTCTGTTATCGGGCATCGAAGTTGGCAGACGAGTCGGATGGAGCGCCATTGTTTACAGTTGCTGAAACACATTCCACGAATTCAGGGAGACACGGCCATGCATTGGATAACTGTAGTAGGTTAGTGGAGGACGATAGTGATGGCGTGGTGCACATGCCGAAGGAAGAGGAGGAAGCACATGAGGACGACTGGCAATTTCTTTGA

Protein sequence

MGRGKDLNSTCSSASTTDSIDRTQKSVCPELGSKDHASSKASLWSSFLTSTLTIFEHHKEPSVSEKKTFHSRQNVWTTIRKVMTSGSMRRLQERILGSHRSGVYSSGGDIWLLGVCHKISQDQAPDDVTSNGAAGFELDFSSRILITYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQALLFHRLGRSWRKTSRKPLDKEYVEILHLFGDSEKSAFSIHNILQAGRAYDLAAGSWVGPYAMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSKGQLDWTPILLLVPLVLGLEKINSRYILSLKTTFTFPQSLGILGGKPSVSTYIVGVQDENAFYLDPHEVQQVVNIDKDDLDADTSSYHCNVIRHIPLESMDPSLAIGFYCRDKDDFDDFCYRASKLADESDGAPLFTVAETHSTNSGRHGHALDNCSRLVEDDSDGVVHMPKEEEEAHEDDWQFL
Homology
BLAST of Csor.00g159870 vs. ExPASy Swiss-Prot
Match: A2Q1V6 (Cysteine protease ATG4 OS=Medicago truncatula OX=3880 GN=ATG4 PE=3 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 3.2e-174
Identity = 308/476 (64.71%), Postives = 370/476 (77.73%), Query Frame = 0

Query: 11  CSSASTTDSIDRTQKSVCPELGSKDHASSKASLWSSFLTSTLTIFEHHKEPSVSEKKTFH 70
           CSS S+T+ +D TQ     + GS D    KASLWS+F TS  ++ E + E S SEKKT H
Sbjct: 15  CSSKSSTEIVDNTQVPASSKAGSSDSKFPKASLWSTFFTSGFSVDETYSESSSSEKKTVH 74

Query: 71  SRQNVW-TTIRKVMTSGSMRRLQERILGSHRSGVYSSGGDIWLLGVCHKISQDQAPDDV- 130
           SR + W   +RKV++ GSMRR QER+LGS R+ V SS GDIWLLGVCHKISQ ++  DV 
Sbjct: 75  SRNSGWAAAVRKVVSGGSMRRFQERVLGSCRTDVSSSDGDIWLLGVCHKISQHESTGDVD 134

Query: 131 TSNGAAGFELDFSSRILITYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQALLFHRLGR 190
             N  A FE DF SRILITYRKGF+ I+DSKYTSDVNWGCMLRSSQMLVAQALLFH+LGR
Sbjct: 135 IRNVFAAFEQDFFSRILITYRKGFDAIEDSKYTSDVNWGCMLRSSQMLVAQALLFHKLGR 194

Query: 191 SWRKTSRKPLDKEYVEILHLFGDSEKSAFSIHNILQAGRAYDLAAGSWVGPYAMCRSWET 250
           SWRKT  KP+DKEY++IL LFGDSE +AFSIHN+LQAG+ Y LA GSWVGPYAMCR+WE 
Sbjct: 195 SWRKTVDKPVDKEYIDILQLFGDSEAAAFSIHNLLQAGKGYGLAVGSWVGPYAMCRTWEV 254

Query: 251 LVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSKGQLDWTPIL 310
           L R +RE     +Q LPMAIY+VSGDEDGERGGAPV+ I+DA + C EFS+G + WTP+L
Sbjct: 255 LARNQREKNEQGEQLLPMAIYVVSGDEDGERGGAPVVCIEDACKRCLEFSRGLVPWTPLL 314

Query: 311 LLVPLVLGLEKINSRYILSLKTTFTFPQSLGILGGKPSVSTYIVGVQDENAFYLDPHEVQ 370
           LLVPLVLGL+K+N RYI  L++TF FPQSLGILGGKP  STYI+GVQ++ AFYLDPHEV+
Sbjct: 315 LLVPLVLGLDKVNLRYIPLLQSTFKFPQSLGILGGKPGASTYIIGVQNDKAFYLDPHEVK 374

Query: 371 QVVNIDKDDLDADTSSYHCNVIRHIPLESMDPSLAIGFYCRDKDDFDDFCYRASKLADES 430
            VVNI  D  + +TSSYHCN+ RH+PL+S+DPSLAIGFYCRDKDDFDDFC RA+KLA+ES
Sbjct: 375 PVVNITGDTQEPNTSSYHCNISRHMPLDSIDPSLAIGFYCRDKDDFDDFCSRATKLAEES 434

Query: 431 DGAPLFTVAETHSTNSGRHGHALD-NCSRLVEDDSDGVVHMPKEEEEAHEDDWQFL 484
           +GAPLFTVA++ S       +++  + +R  EDDS   + M    +  +EDDWQFL
Sbjct: 435 NGAPLFTVAQSRSLPMQVTSNSVSGDDTRFEEDDS---LSMNLVNDAGNEDDWQFL 487

BLAST of Csor.00g159870 vs. ExPASy Swiss-Prot
Match: Q8S929 (Cysteine protease ATG4a OS=Arabidopsis thaliana OX=3702 GN=ATG4A PE=2 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 2.2e-154
Identity = 277/477 (58.07%), Postives = 354/477 (74.21%), Query Frame = 0

Query: 11  CSSASTTDSIDRTQKSVCPELGSKDHASSKASLWSSFLTSTLTIFEHHKEPSVSEKKTFH 70
           CSS+S +D+ D++   +  + G  D+  SK +LWS+  TS+ ++ + ++E S S  K   
Sbjct: 13  CSSSSKSDTHDKS--PLVSDSGPSDN-KSKFTLWSNVFTSSSSVSQPYRESSTSGHKQVC 72

Query: 71  SRQNVWTTI--RKVMTSGSMRRLQERILGSHRSGVYSSGGDIWLLGVCHKISQDQAPDDV 130
           + +N WT    R  M SG++RR QER+LG +R+G+ S+  D+WLLGVC+KIS D+   + 
Sbjct: 73  TTRNGWTAFVKRVSMASGAIRRFQERVLGPNRTGLPSTTSDVWLLGVCYKISADENSGET 132

Query: 131 -TSNGAAGFELDFSSRILITYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQALLFHRLG 190
            T    A  +LDFSS+IL+TYRKGF   +D+ YTSDVNWGCM+RSSQML AQALLFHRLG
Sbjct: 133 DTGTVLAALQLDFSSKILMTYRKGFEPFRDTTYTSDVNWGCMIRSSQMLFAQALLFHRLG 192

Query: 191 RSWRKTSRKPLDKEYVEILHLFGDSEKSAFSIHNILQAGRAYDLAAGSWVGPYAMCRSWE 250
           R+W K S  P ++EY+E L  FGDSE SAFSIHN++ AG +Y LAAGSWVGPYA+CR+WE
Sbjct: 193 RAWTKKSELP-EQEYLETLEPFGDSEPSAFSIHNLIIAGASYGLAAGSWVGPYAICRAWE 252

Query: 251 TLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSKGQLDWTPI 310
           +L   KR+  + ++Q LPMA++IVSG EDGERGGAP+L I+DA++ C EFSKGQ +WTPI
Sbjct: 253 SLACKKRKQTDSKNQTLPMAVHIVSGSEDGERGGAPILCIEDATKSCLEFSKGQSEWTPI 312

Query: 311 LLLVPLVLGLEKINSRYILSLKTTFTFPQSLGILGGKPSVSTYIVGVQDENAFYLDPHEV 370
           +LLVPLVLGL+ +N RYI SL  TFTFPQS+GILGGKP  STYIVGVQ++  FYLDPHEV
Sbjct: 313 ILLVPLVLGLDSVNPRYIPSLVATFTFPQSVGILGGKPGASTYIVGVQEDKGFYLDPHEV 372

Query: 371 QQVVNIDKDDLDADTSSYHCNVIRHIPLESMDPSLAIGFYCRDKDDFDDFCYRASKLADE 430
           QQVV ++K+  D DTSSYHCNV+R++PLES+DPSLA+GFYCRDKDDFDDFC RA KLA+E
Sbjct: 373 QQVVTVNKETPDVDTSSYHCNVLRYVPLESLDPSLALGFYCRDKDDFDDFCLRALKLAEE 432

Query: 431 SDGAPLFTVAETHST-NSGRHGHALDNCSRLVEDDSDGVVHMPKEEEEAHEDDWQFL 484
           S+GAPLFTV +TH+  N   +G A        +DDS          E+  EDDWQ L
Sbjct: 433 SNGAPLFTVTQTHTAINQSNYGFA--------DDDS----------EDEREDDWQML 467

BLAST of Csor.00g159870 vs. ExPASy Swiss-Prot
Match: Q9M1Y0 (Cysteine protease ATG4b OS=Arabidopsis thaliana OX=3702 GN=ATG4B PE=1 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 2.9e-146
Identity = 262/479 (54.70%), Postives = 341/479 (71.19%), Query Frame = 0

Query: 9   STCSSASTTDSID-RTQKSVCPELGSKDHASSKASLWSSFLTSTLTIFEHHKEPSVSEKK 68
           S CSS+ST++  D  +  S+  +  S D+  S  +L S  + S+  + +  +E S S   
Sbjct: 11  SKCSSSSTSEKRDISSPTSLVSDSASSDN-KSNLTLCSDVVASSSPVSQLCREASTSGHN 70

Query: 69  TFHSRQNVWTTIRKV--MTSGSMRRLQERILGSHRSGVYSSGGDIWLLGVCHKISQDQAP 128
              +  + WT I K   M SG++RR Q+R+LG  R+G+ SS  +IWLLGVC+KIS+ ++ 
Sbjct: 71  PVCTTHSSWTVILKTASMASGAIRRFQDRVLGPSRTGISSSTSEIWLLGVCYKISEGESS 130

Query: 129 DDVTSNGA-AGFELDFSSRILITYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQALLFH 188
           ++  +    A F  DFSS IL+TYR+GF  I D+ YTSDVNWGCMLRS QML AQALLF 
Sbjct: 131 EEADAGRVLAAFRQDFSSLILMTYRRGFEPIGDTTYTSDVNWGCMLRSGQMLFAQALLFQ 190

Query: 189 RLGRSWRKTSRKPLDKEYVEILHLFGDSEKSAFSIHNILQAGRAYDLAAGSWVGPYAMCR 248
           RLGRSWRK   +P D++Y+EIL LFGD+E SAFSIHN++ AG +Y LAAGSWVGPYA+CR
Sbjct: 191 RLGRSWRKKDSEPADEKYLEILELFGDTEASAFSIHNLILAGESYGLAAGSWVGPYAVCR 250

Query: 249 SWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSKGQLDW 308
           SWE+L R  +E  + + +   MA++IVSG EDGERGGAP+L I+D ++ C EFS+G+ +W
Sbjct: 251 SWESLARKNKEETDDKHKSFSMAVHIVSGSEDGERGGAPILCIEDVTKTCLEFSEGETEW 310

Query: 309 TPILLLVPLVLGLEKINSRYILSLKTTFTFPQSLGILGGKPSVSTYIVGVQDENAFYLDP 368
            PILLLVPLVLGL+++N RYI SL  TFTFPQSLGILGGKP  STYIVGVQ++  FYLDP
Sbjct: 311 PPILLLVPLVLGLDRVNPRYIPSLIATFTFPQSLGILGGKPGASTYIVGVQEDKGFYLDP 370

Query: 369 HEVQQVVNIDKDDLDADTSSYHCNVIRHIPLESMDPSLAIGFYCRDKDDFDDFCYRASKL 428
           H+VQQVV + K++ D DTSSYHCN +R++PLES+DPSLA+GFYC+ KDDFDDFC RA+KL
Sbjct: 371 HDVQQVVTVKKENQDVDTSSYHCNTLRYVPLESLDPSLALGFYCQHKDDFDDFCIRATKL 430

Query: 429 ADESDGAPLFTVAETHSTNSGRHGHALDNCSRLVEDDSDGVVHMPKEEEEAHEDDWQFL 484
           A +S+GAPLFTV ++H  N         +C   + + S       +   E HEDDWQ L
Sbjct: 431 AGDSNGAPLFTVTQSHRRN---------DCG--IAETSSSTETSTEISGEEHEDDWQLL 477

BLAST of Csor.00g159870 vs. ExPASy Swiss-Prot
Match: Q2XPP4 (Cysteine protease ATG4B OS=Oryza sativa subsp. indica OX=39946 GN=ATG4B PE=1 SV=2)

HSP 1 Score: 498.0 bits (1281), Expect = 1.2e-139
Identity = 255/449 (56.79%), Postives = 321/449 (71.49%), Query Frame = 0

Query: 39  SKASLWSSFLTSTLTIFEHHKEPSVSEKKTFHSRQNVW-TTIRKVMTSGSMRRLQERILG 98
           SK S+ S    S   IFE H++ S ++     S    W   +R+++ SGSM     R LG
Sbjct: 40  SKTSILSCVFNSPFNIFEAHQDSSANKSPKSSSGSYDWLRVLRRIVCSGSM----WRFLG 99

Query: 99  SHRSGVYSSGGDIWLLGVCHKISQDQAPDDVTS-NGAAGFELDFSSRILITYRKGFNVIQ 158
           + +     +  D+W LG C+K+S +++  D  S +G A F  DFSSRI ITYR+GF+ I 
Sbjct: 100 TSK---VLTSSDVWFLGKCYKLSSEESSSDSDSESGHATFLEDFSSRIWITYRRGFDAIS 159

Query: 159 DSKYTSDVNWGCMLRSSQMLVAQALLFHRLGRSWRKTSRKPLDKEYVEILHLFGDSEKSA 218
           DSKYTSDVNWGCM+RSSQMLVAQAL+FH LGRSWR+ S KP + EY+ ILH+FGDSE  A
Sbjct: 160 DSKYTSDVNWGCMVRSSQMLVAQALIFHHLGRSWRRPSEKPYNPEYIGILHMFGDSEACA 219

Query: 219 FSIHNILQAGRAYDLAAGSWVGPYAMCRSWETLVRLKRETPNLQD--QQLPMAIYIVSGD 278
           FSIHN+LQAG +Y LAAGSWVGPYAMCR+W+TLVR  RE   + D  +  PMA+Y+VSGD
Sbjct: 220 FSIHNLLQAGNSYGLAAGSWVGPYAMCRAWQTLVRTNREQHEVVDGNESFPMALYVVSGD 279

Query: 279 EDGERGGAPVLYIDDASRHCFEFSKGQLDWTPILLLVPLVLGLEKINSRYILSLKTTFTF 338
           EDGERGGAPV+ ID A++ C +F+KGQ  W+PILLLVPLVLGL+KIN RYI  LK TFTF
Sbjct: 280 EDGERGGAPVVCIDVAAQLCCDFNKGQSTWSPILLLVPLVLGLDKINPRYIPLLKETFTF 339

Query: 339 PQSLGILGGKPSVSTYIVGVQDENAFYLDPHEVQQVVNIDKDDLDADTSSYHCNVIRHIP 398
           PQSLGILGGKP  STYI GVQD+ A YLDPHEVQ  V+I  D+++ADTSSYHC+ +R + 
Sbjct: 340 PQSLGILGGKPGTSTYIAGVQDDRALYLDPHEVQMAVDIAADNIEADTSSYHCSTVRDLA 399

Query: 399 LESMDPSLAIGFYCRDKDDFDDFCYRASKLADESDGAPLFTVAETHSTNSGRHGHALDNC 458
           L+ +DPSLAIGFYCRDKDDFDDFC RA++L D+++GAPLFTV +  S    +  +  D+ 
Sbjct: 400 LDLIDPSLAIGFYCRDKDDFDDFCSRATELVDKANGAPLFTVVQ--SVQPSKQMYNQDDV 459

Query: 459 SRLVEDDSDGVVHMPKEEEEAHEDDWQFL 484
             +  D +  V  +    E   E++WQ L
Sbjct: 460 LGISGDGNINVEDLDASGETG-EEEWQIL 478

BLAST of Csor.00g159870 vs. ExPASy Swiss-Prot
Match: Q7XPW8 (Cysteine protease ATG4B OS=Oryza sativa subsp. japonica OX=39947 GN=ATG4B PE=2 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 3.4e-139
Identity = 254/449 (56.57%), Postives = 321/449 (71.49%), Query Frame = 0

Query: 39  SKASLWSSFLTSTLTIFEHHKEPSVSEKKTFHSRQNVWT-TIRKVMTSGSMRRLQERILG 98
           SK S+ S    S   IFE H++ S ++     S    W+  +R+++ SGSM     R LG
Sbjct: 40  SKTSILSCVFNSPFNIFEAHQDSSANKSPKSSSGSYDWSRVLRRIVCSGSM----WRFLG 99

Query: 99  SHRSGVYSSGGDIWLLGVCHKISQDQAPDDVTS-NGAAGFELDFSSRILITYRKGFNVIQ 158
           + +     +  D+W LG C+K+S +++  D  S +G A F  DFSSRI ITYR+GF+ I 
Sbjct: 100 TSK---VLTSSDVWFLGKCYKLSSEESSSDSDSESGHATFLEDFSSRIWITYRRGFDAIS 159

Query: 159 DSKYTSDVNWGCMLRSSQMLVAQALLFHRLGRSWRKTSRKPLDKEYVEILHLFGDSEKSA 218
           DSKYTSDVNWGCM+RSSQMLVAQAL+FH LGRSWR+   KP + EY+ ILH+FGDSE  A
Sbjct: 160 DSKYTSDVNWGCMVRSSQMLVAQALIFHHLGRSWRRPLEKPYNPEYIGILHMFGDSEACA 219

Query: 219 FSIHNILQAGRAYDLAAGSWVGPYAMCRSWETLVRLKRETPNLQD--QQLPMAIYIVSGD 278
           FSIHN+LQAG +Y LAAGSWVGPYAMCR+W+TLVR  RE   + D  +  PMA+Y+VSGD
Sbjct: 220 FSIHNLLQAGNSYGLAAGSWVGPYAMCRAWQTLVRTNREQHEVVDGNESFPMALYVVSGD 279

Query: 279 EDGERGGAPVLYIDDASRHCFEFSKGQLDWTPILLLVPLVLGLEKINSRYILSLKTTFTF 338
           EDGERGGAPV+ ID A++ C +F+KGQ  W+PILLLVPLVLGL+KIN RYI  LK TFTF
Sbjct: 280 EDGERGGAPVVCIDVAAQLCCDFNKGQSTWSPILLLVPLVLGLDKINPRYIPLLKETFTF 339

Query: 339 PQSLGILGGKPSVSTYIVGVQDENAFYLDPHEVQQVVNIDKDDLDADTSSYHCNVIRHIP 398
           PQSLGILGGKP  STYI GVQD+ A YLDPHEVQ  V+I  D+++ADTSSYHC+ +R + 
Sbjct: 340 PQSLGILGGKPGTSTYIAGVQDDRALYLDPHEVQMAVDIAADNIEADTSSYHCSTVRDLA 399

Query: 399 LESMDPSLAIGFYCRDKDDFDDFCYRASKLADESDGAPLFTVAETHSTNSGRHGHALDNC 458
           L+ +DPSLAIGFYCRDKDDFDDFC RA++L D+++GAPLFTV +  S    +  +  D+ 
Sbjct: 400 LDLIDPSLAIGFYCRDKDDFDDFCSRATELVDKANGAPLFTVVQ--SVQPSKQMYNQDDV 459

Query: 459 SRLVEDDSDGVVHMPKEEEEAHEDDWQFL 484
             +  D +  V  +    E   E++WQ L
Sbjct: 460 LGISGDGNINVEDLDASGETG-EEEWQIL 478

BLAST of Csor.00g159870 vs. NCBI nr
Match: KAG6579717.1 (Cysteine protease ATG4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 981 bits (2535), Expect = 0.0
Identity = 483/483 (100.00%), Postives = 483/483 (100.00%), Query Frame = 0

Query: 1   MGRGKDLNSTCSSASTTDSIDRTQKSVCPELGSKDHASSKASLWSSFLTSTLTIFEHHKE 60
           MGRGKDLNSTCSSASTTDSIDRTQKSVCPELGSKDHASSKASLWSSFLTSTLTIFEHHKE
Sbjct: 1   MGRGKDLNSTCSSASTTDSIDRTQKSVCPELGSKDHASSKASLWSSFLTSTLTIFEHHKE 60

Query: 61  PSVSEKKTFHSRQNVWTTIRKVMTSGSMRRLQERILGSHRSGVYSSGGDIWLLGVCHKIS 120
           PSVSEKKTFHSRQNVWTTIRKVMTSGSMRRLQERILGSHRSGVYSSGGDIWLLGVCHKIS
Sbjct: 61  PSVSEKKTFHSRQNVWTTIRKVMTSGSMRRLQERILGSHRSGVYSSGGDIWLLGVCHKIS 120

Query: 121 QDQAPDDVTSNGAAGFELDFSSRILITYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQA 180
           QDQAPDDVTSNGAAGFELDFSSRILITYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQA
Sbjct: 121 QDQAPDDVTSNGAAGFELDFSSRILITYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQA 180

Query: 181 LLFHRLGRSWRKTSRKPLDKEYVEILHLFGDSEKSAFSIHNILQAGRAYDLAAGSWVGPY 240
           LLFHRLGRSWRKTSRKPLDKEYVEILHLFGDSEKSAFSIHNILQAGRAYDLAAGSWVGPY
Sbjct: 181 LLFHRLGRSWRKTSRKPLDKEYVEILHLFGDSEKSAFSIHNILQAGRAYDLAAGSWVGPY 240

Query: 241 AMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSKG 300
           AMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSKG
Sbjct: 241 AMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSKG 300

Query: 301 QLDWTPILLLVPLVLGLEKINSRYILSLKTTFTFPQSLGILGGKPSVSTYIVGVQDENAF 360
           QLDWTPILLLVPLVLGLEKINSRYILSLKTTFTFPQSLGILGGKPSVSTYIVGVQDENAF
Sbjct: 301 QLDWTPILLLVPLVLGLEKINSRYILSLKTTFTFPQSLGILGGKPSVSTYIVGVQDENAF 360

Query: 361 YLDPHEVQQVVNIDKDDLDADTSSYHCNVIRHIPLESMDPSLAIGFYCRDKDDFDDFCYR 420
           YLDPHEVQQVVNIDKDDLDADTSSYHCNVIRHIPLESMDPSLAIGFYCRDKDDFDDFCYR
Sbjct: 361 YLDPHEVQQVVNIDKDDLDADTSSYHCNVIRHIPLESMDPSLAIGFYCRDKDDFDDFCYR 420

Query: 421 ASKLADESDGAPLFTVAETHSTNSGRHGHALDNCSRLVEDDSDGVVHMPKEEEEAHEDDW 480
           ASKLADESDGAPLFTVAETHSTNSGRHGHALDNCSRLVEDDSDGVVHMPKEEEEAHEDDW
Sbjct: 421 ASKLADESDGAPLFTVAETHSTNSGRHGHALDNCSRLVEDDSDGVVHMPKEEEEAHEDDW 480

Query: 481 QFL 483
           QFL
Sbjct: 481 QFL 483

BLAST of Csor.00g159870 vs. NCBI nr
Match: KAG7017160.1 (Cysteine protease ATG4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 979 bits (2532), Expect = 0.0
Identity = 482/483 (99.79%), Postives = 483/483 (100.00%), Query Frame = 0

Query: 1   MGRGKDLNSTCSSASTTDSIDRTQKSVCPELGSKDHASSKASLWSSFLTSTLTIFEHHKE 60
           MGRGKDLNSTCSSASTTDSIDRTQKSVCPELGSKDHASSKASLWSSFLTSTLTIFEHHKE
Sbjct: 1   MGRGKDLNSTCSSASTTDSIDRTQKSVCPELGSKDHASSKASLWSSFLTSTLTIFEHHKE 60

Query: 61  PSVSEKKTFHSRQNVWTTIRKVMTSGSMRRLQERILGSHRSGVYSSGGDIWLLGVCHKIS 120
           PSVSEKKTFHSRQNVWTTIRKVMTSGSMRRLQERILGSHRSGVYSSGGDIWLLGVCHKIS
Sbjct: 61  PSVSEKKTFHSRQNVWTTIRKVMTSGSMRRLQERILGSHRSGVYSSGGDIWLLGVCHKIS 120

Query: 121 QDQAPDDVTSNGAAGFELDFSSRILITYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQA 180
           QDQAPDDVTSNGAAGFELDFSSRILITYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQA
Sbjct: 121 QDQAPDDVTSNGAAGFELDFSSRILITYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQA 180

Query: 181 LLFHRLGRSWRKTSRKPLDKEYVEILHLFGDSEKSAFSIHNILQAGRAYDLAAGSWVGPY 240
           LLFHRLGRSWRKTSRKPLDKEYVEILHLFGDSEKSAFSIHNILQAGRAYDLAAGSWVGPY
Sbjct: 181 LLFHRLGRSWRKTSRKPLDKEYVEILHLFGDSEKSAFSIHNILQAGRAYDLAAGSWVGPY 240

Query: 241 AMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSKG 300
           AMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSKG
Sbjct: 241 AMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSKG 300

Query: 301 QLDWTPILLLVPLVLGLEKINSRYILSLKTTFTFPQSLGILGGKPSVSTYIVGVQDENAF 360
           QLDWTPILLLVPLVLGLEKINSRYILSLKTTFTFPQSLGILGGKPSVSTYIVGVQDENAF
Sbjct: 301 QLDWTPILLLVPLVLGLEKINSRYILSLKTTFTFPQSLGILGGKPSVSTYIVGVQDENAF 360

Query: 361 YLDPHEVQQVVNIDKDDLDADTSSYHCNVIRHIPLESMDPSLAIGFYCRDKDDFDDFCYR 420
           YLDPHEVQQVVNIDKDDLDADTSSYHCNVIRHIPLESMDPSLAIGFYCRDKDDFDDFCYR
Sbjct: 361 YLDPHEVQQVVNIDKDDLDADTSSYHCNVIRHIPLESMDPSLAIGFYCRDKDDFDDFCYR 420

Query: 421 ASKLADESDGAPLFTVAETHSTNSGRHGHALDNCSRLVEDDSDGVVHMPKEEEEAHEDDW 480
           ASKLADESDGAPLFTVAETHSTNSGRHGHALDNCSRLVEDD+DGVVHMPKEEEEAHEDDW
Sbjct: 421 ASKLADESDGAPLFTVAETHSTNSGRHGHALDNCSRLVEDDTDGVVHMPKEEEEAHEDDW 480

Query: 481 QFL 483
           QFL
Sbjct: 481 QFL 483

BLAST of Csor.00g159870 vs. NCBI nr
Match: XP_022928925.1 (cysteine protease ATG4-like [Cucurbita moschata] >XP_022928926.1 cysteine protease ATG4-like [Cucurbita moschata])

HSP 1 Score: 968 bits (2502), Expect = 0.0
Identity = 475/483 (98.34%), Postives = 480/483 (99.38%), Query Frame = 0

Query: 1   MGRGKDLNSTCSSASTTDSIDRTQKSVCPELGSKDHASSKASLWSSFLTSTLTIFEHHKE 60
           MGRGKDLNSTCSSASTTDSIDRTQ+SVCPELGSKDH SSKASLWSSFLTSTLTIFEHHKE
Sbjct: 1   MGRGKDLNSTCSSASTTDSIDRTQQSVCPELGSKDHVSSKASLWSSFLTSTLTIFEHHKE 60

Query: 61  PSVSEKKTFHSRQNVWTTIRKVMTSGSMRRLQERILGSHRSGVYSSGGDIWLLGVCHKIS 120
           PSVSEKKTFHSRQNVWTT+RKVMTSGSMRRLQERILGSHRSGVYSSGGDIWLLGVCHKIS
Sbjct: 61  PSVSEKKTFHSRQNVWTTVRKVMTSGSMRRLQERILGSHRSGVYSSGGDIWLLGVCHKIS 120

Query: 121 QDQAPDDVTSNGAAGFELDFSSRILITYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQA 180
           QDQAPDDVTSNGAAGFELDFSSRILITYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQA
Sbjct: 121 QDQAPDDVTSNGAAGFELDFSSRILITYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQA 180

Query: 181 LLFHRLGRSWRKTSRKPLDKEYVEILHLFGDSEKSAFSIHNILQAGRAYDLAAGSWVGPY 240
           LLFHRLGRSWRKTSRKPLDKEYVEILHLFGDSEKSAFSIHNILQAGRAYDLAAGSWVGPY
Sbjct: 181 LLFHRLGRSWRKTSRKPLDKEYVEILHLFGDSEKSAFSIHNILQAGRAYDLAAGSWVGPY 240

Query: 241 AMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSKG 300
           AMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSKG
Sbjct: 241 AMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSKG 300

Query: 301 QLDWTPILLLVPLVLGLEKINSRYILSLKTTFTFPQSLGILGGKPSVSTYIVGVQDENAF 360
           QLDWTPILLLVPLVLGLEKIN RYILSLKTTFTFPQSLGILGGKPSVSTYIVGVQDENAF
Sbjct: 301 QLDWTPILLLVPLVLGLEKINPRYILSLKTTFTFPQSLGILGGKPSVSTYIVGVQDENAF 360

Query: 361 YLDPHEVQQVVNIDKDDLDADTSSYHCNVIRHIPLESMDPSLAIGFYCRDKDDFDDFCYR 420
           YLDPHEVQQVVNIDKDDLDADTSSYHCNVIRHIPLESMDPSLAIGFYCRDKDDFDDFCYR
Sbjct: 361 YLDPHEVQQVVNIDKDDLDADTSSYHCNVIRHIPLESMDPSLAIGFYCRDKDDFDDFCYR 420

Query: 421 ASKLADESDGAPLFTVAETHSTNSGRHGHALDNCSRLVEDDSDGVVHMPKEEEEAHEDDW 480
           ASKLAD+SDGAPLFTVAETHSTNSGRHG+ALDNCSRLVEDD+DGVVHMP EEEEAHEDDW
Sbjct: 421 ASKLADKSDGAPLFTVAETHSTNSGRHGNALDNCSRLVEDDTDGVVHMPNEEEEAHEDDW 480

Query: 481 QFL 483
           QFL
Sbjct: 481 QFL 483

BLAST of Csor.00g159870 vs. NCBI nr
Match: XP_023551477.1 (cysteine protease ATG4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 957 bits (2473), Expect = 0.0
Identity = 468/483 (96.89%), Postives = 476/483 (98.55%), Query Frame = 0

Query: 1   MGRGKDLNSTCSSASTTDSIDRTQKSVCPELGSKDHASSKASLWSSFLTSTLTIFEHHKE 60
           MGRGKDLNSTCSS STTD+IDRTQ+SVCPELGSKDH SSKASLWSSFLTSTLTIFEHHKE
Sbjct: 1   MGRGKDLNSTCSSESTTDTIDRTQQSVCPELGSKDHVSSKASLWSSFLTSTLTIFEHHKE 60

Query: 61  PSVSEKKTFHSRQNVWTTIRKVMTSGSMRRLQERILGSHRSGVYSSGGDIWLLGVCHKIS 120
           PSVSEKKTFHSRQNVWTT+RKVMTSGSMRRLQERILGSHRSGVYSSGGDIWLLGVCHKIS
Sbjct: 61  PSVSEKKTFHSRQNVWTTVRKVMTSGSMRRLQERILGSHRSGVYSSGGDIWLLGVCHKIS 120

Query: 121 QDQAPDDVTSNGAAGFELDFSSRILITYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQA 180
           QDQAPDDVTSNG+AGFELDFSSRILITYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQA
Sbjct: 121 QDQAPDDVTSNGSAGFELDFSSRILITYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQA 180

Query: 181 LLFHRLGRSWRKTSRKPLDKEYVEILHLFGDSEKSAFSIHNILQAGRAYDLAAGSWVGPY 240
           LLFHRLGRSWRKTSRKPLDKEYVE+LHLFGDSEKSAFSIHN+LQAGRAYDLAAGSWVGPY
Sbjct: 181 LLFHRLGRSWRKTSRKPLDKEYVEVLHLFGDSEKSAFSIHNLLQAGRAYDLAAGSWVGPY 240

Query: 241 AMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSKG 300
           AMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSKG
Sbjct: 241 AMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSKG 300

Query: 301 QLDWTPILLLVPLVLGLEKINSRYILSLKTTFTFPQSLGILGGKPSVSTYIVGVQDENAF 360
           QLDWTPILLLVPLVLGLEKIN RYILSLKTTFTFPQSLGILGGKPSVSTYIVGVQDENAF
Sbjct: 301 QLDWTPILLLVPLVLGLEKINPRYILSLKTTFTFPQSLGILGGKPSVSTYIVGVQDENAF 360

Query: 361 YLDPHEVQQVVNIDKDDLDADTSSYHCNVIRHIPLESMDPSLAIGFYCRDKDDFDDFCYR 420
           YLDPHEVQQVVNIDKDDLDADTSSYHCNVIRHIPLESMDPSLAIGFYCRDKDDFDDFCYR
Sbjct: 361 YLDPHEVQQVVNIDKDDLDADTSSYHCNVIRHIPLESMDPSLAIGFYCRDKDDFDDFCYR 420

Query: 421 ASKLADESDGAPLFTVAETHSTNSGRHGHALDNCSRLVEDDSDGVVHMPKEEEEAHEDDW 480
           ASKLADESDGAPLFTVAETHSTNSGR G+ALDNCSRL E D+DGVVHMP EEEEAHEDDW
Sbjct: 421 ASKLADESDGAPLFTVAETHSTNSGRQGNALDNCSRLAEGDTDGVVHMPNEEEEAHEDDW 480

Query: 481 QFL 483
           QFL
Sbjct: 481 QFL 483

BLAST of Csor.00g159870 vs. NCBI nr
Match: XP_038874853.1 (cysteine protease ATG4-like [Benincasa hispida])

HSP 1 Score: 886 bits (2290), Expect = 0.0
Identity = 437/484 (90.29%), Postives = 457/484 (94.42%), Query Frame = 0

Query: 1   MGRGKDLNSTCSSASTTDSIDRTQKSVCPELGSKDHASSKASLWSSFLTSTLTIFEHHKE 60
           MGRGKDLNSTCSS STT+ IDRT +SVCPELGSK+H SSKASLWS F TST +IFEHHKE
Sbjct: 1   MGRGKDLNSTCSSESTTNVIDRTHQSVCPELGSKNHISSKASLWSGFFTSTFSIFEHHKE 60

Query: 61  PSVSEKKTFHSRQNVWTTIRKVMTSGSMRRLQERILGSHRSGVYSSGGDIWLLGVCHKIS 120
            SVSEKK FHSR NVW T+RKVMTSGSMRR+QERILGS RSGVYSSGGDIWLLGVCHKIS
Sbjct: 61  SSVSEKKAFHSRHNVWATVRKVMTSGSMRRIQERILGSRRSGVYSSGGDIWLLGVCHKIS 120

Query: 121 QDQAPDD-VTSNGAAGFELDFSSRILITYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQ 180
           QDQAPDD VTS G AG+ELDFSSRIL+TYRKGFN IQDSKYTSDVNWGCMLRSSQMLVAQ
Sbjct: 121 QDQAPDDAVTSTGTAGYELDFSSRILMTYRKGFNAIQDSKYTSDVNWGCMLRSSQMLVAQ 180

Query: 181 ALLFHRLGRSWRKTSRKPLDKEYVEILHLFGDSEKSAFSIHNILQAGRAYDLAAGSWVGP 240
           ALLFHRLGRSWRKTS+KPLDKEYVEILHLFGDSE SAFSIHN+LQAGRAYDLAAGSWVGP
Sbjct: 181 ALLFHRLGRSWRKTSQKPLDKEYVEILHLFGDSETSAFSIHNLLQAGRAYDLAAGSWVGP 240

Query: 241 YAMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSK 300
           YAMCRSWETLVRLKRETPNLQ+QQLPMAIYIVSGDEDGERGGAPVL IDDASRHCFEFSK
Sbjct: 241 YAMCRSWETLVRLKRETPNLQEQQLPMAIYIVSGDEDGERGGAPVLCIDDASRHCFEFSK 300

Query: 301 GQLDWTPILLLVPLVLGLEKINSRYILSLKTTFTFPQSLGILGGKPSVSTYIVGVQDENA 360
           GQLDW+PILLLVPLVLGLEKIN RYI SL+TTFTFPQSLGILGGKP  STYIVGVQDENA
Sbjct: 301 GQLDWSPILLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENA 360

Query: 361 FYLDPHEVQQVVNIDKDDLDADTSSYHCNVIRHIPLESMDPSLAIGFYCRDKDDFDDFCY 420
           FYLDPH+VQQVVNIDKDDL+ADTSSYHCNVIRHIPLES+DPSLAIGFYCRDKDDFD+FCY
Sbjct: 361 FYLDPHDVQQVVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDNFCY 420

Query: 421 RASKLADESDGAPLFTVAETHSTNSGRHGHALDNCSRLVEDDSDGVVHMPKEEEEAHEDD 480
           RASKLADESDGAPLFTVAETHSTNSGRHG AL++ SRLVEDD+DGVVHMP EE  AHEDD
Sbjct: 421 RASKLADESDGAPLFTVAETHSTNSGRHGSALNDRSRLVEDDTDGVVHMPNEE--AHEDD 480

Query: 481 WQFL 483
           WQFL
Sbjct: 481 WQFL 482

BLAST of Csor.00g159870 vs. ExPASy TrEMBL
Match: A0A6J1ELN4 (Cysteine protease OS=Cucurbita moschata OX=3662 GN=LOC111435689 PE=3 SV=1)

HSP 1 Score: 968 bits (2502), Expect = 0.0
Identity = 475/483 (98.34%), Postives = 480/483 (99.38%), Query Frame = 0

Query: 1   MGRGKDLNSTCSSASTTDSIDRTQKSVCPELGSKDHASSKASLWSSFLTSTLTIFEHHKE 60
           MGRGKDLNSTCSSASTTDSIDRTQ+SVCPELGSKDH SSKASLWSSFLTSTLTIFEHHKE
Sbjct: 1   MGRGKDLNSTCSSASTTDSIDRTQQSVCPELGSKDHVSSKASLWSSFLTSTLTIFEHHKE 60

Query: 61  PSVSEKKTFHSRQNVWTTIRKVMTSGSMRRLQERILGSHRSGVYSSGGDIWLLGVCHKIS 120
           PSVSEKKTFHSRQNVWTT+RKVMTSGSMRRLQERILGSHRSGVYSSGGDIWLLGVCHKIS
Sbjct: 61  PSVSEKKTFHSRQNVWTTVRKVMTSGSMRRLQERILGSHRSGVYSSGGDIWLLGVCHKIS 120

Query: 121 QDQAPDDVTSNGAAGFELDFSSRILITYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQA 180
           QDQAPDDVTSNGAAGFELDFSSRILITYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQA
Sbjct: 121 QDQAPDDVTSNGAAGFELDFSSRILITYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQA 180

Query: 181 LLFHRLGRSWRKTSRKPLDKEYVEILHLFGDSEKSAFSIHNILQAGRAYDLAAGSWVGPY 240
           LLFHRLGRSWRKTSRKPLDKEYVEILHLFGDSEKSAFSIHNILQAGRAYDLAAGSWVGPY
Sbjct: 181 LLFHRLGRSWRKTSRKPLDKEYVEILHLFGDSEKSAFSIHNILQAGRAYDLAAGSWVGPY 240

Query: 241 AMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSKG 300
           AMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSKG
Sbjct: 241 AMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSKG 300

Query: 301 QLDWTPILLLVPLVLGLEKINSRYILSLKTTFTFPQSLGILGGKPSVSTYIVGVQDENAF 360
           QLDWTPILLLVPLVLGLEKIN RYILSLKTTFTFPQSLGILGGKPSVSTYIVGVQDENAF
Sbjct: 301 QLDWTPILLLVPLVLGLEKINPRYILSLKTTFTFPQSLGILGGKPSVSTYIVGVQDENAF 360

Query: 361 YLDPHEVQQVVNIDKDDLDADTSSYHCNVIRHIPLESMDPSLAIGFYCRDKDDFDDFCYR 420
           YLDPHEVQQVVNIDKDDLDADTSSYHCNVIRHIPLESMDPSLAIGFYCRDKDDFDDFCYR
Sbjct: 361 YLDPHEVQQVVNIDKDDLDADTSSYHCNVIRHIPLESMDPSLAIGFYCRDKDDFDDFCYR 420

Query: 421 ASKLADESDGAPLFTVAETHSTNSGRHGHALDNCSRLVEDDSDGVVHMPKEEEEAHEDDW 480
           ASKLAD+SDGAPLFTVAETHSTNSGRHG+ALDNCSRLVEDD+DGVVHMP EEEEAHEDDW
Sbjct: 421 ASKLADKSDGAPLFTVAETHSTNSGRHGNALDNCSRLVEDDTDGVVHMPNEEEEAHEDDW 480

Query: 481 QFL 483
           QFL
Sbjct: 481 QFL 483

BLAST of Csor.00g159870 vs. ExPASy TrEMBL
Match: A0A5A7SP82 (Cysteine protease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold340G00200 PE=3 SV=1)

HSP 1 Score: 850 bits (2195), Expect = 2.79e-309
Identity = 419/484 (86.57%), Postives = 443/484 (91.53%), Query Frame = 0

Query: 1   MGRGKDLNSTCSSASTTDSIDRTQKSVCPELGSKDHASSKASLWSSFLTSTLTIFEHHKE 60
           MGRGKDL STCSS +T D IDRT +SVC ELGSK+H SSKASLWS F +S  +I +HHK+
Sbjct: 1   MGRGKDLKSTCSSETTADVIDRTHRSVCSELGSKNHISSKASLWSGFFSSNFSICDHHKD 60

Query: 61  PSVSEKKTFHSRQNVWTTIRKVMTSGSMRRLQERILGSHRSGVYSSGGDIWLLGVCHKIS 120
            SVSEKK FHSR NVW T+RKVMTSGSMRR+QERILGS RSGVY+SGGDIWLLGVCHKIS
Sbjct: 61  SSVSEKKVFHSRHNVWATVRKVMTSGSMRRIQERILGSRRSGVYTSGGDIWLLGVCHKIS 120

Query: 121 QDQAPDDVTSN-GAAGFELDFSSRILITYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQ 180
           QD  PDD  S+ G AGFE DFSSRIL+TYRKGFNVIQDSKYTSDVNWGCMLRSSQMLV+Q
Sbjct: 121 QDHLPDDAASSTGVAGFEQDFSSRILMTYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVSQ 180

Query: 181 ALLFHRLGRSWRKTSRKPLDKEYVEILHLFGDSEKSAFSIHNILQAGRAYDLAAGSWVGP 240
           ALLFHRLGRSWRK S+KP DKEYVEILHLFGDSE SAFSIHN+LQAGRAYDLAAGSWVGP
Sbjct: 181 ALLFHRLGRSWRKPSQKPFDKEYVEILHLFGDSETSAFSIHNLLQAGRAYDLAAGSWVGP 240

Query: 241 YAMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSK 300
           YAMCRSWETLVR KRETP LQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSK
Sbjct: 241 YAMCRSWETLVRSKRETPILQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSK 300

Query: 301 GQLDWTPILLLVPLVLGLEKINSRYILSLKTTFTFPQSLGILGGKPSVSTYIVGVQDENA 360
           GQ DW+PILLLVPLVLGLEKIN RYI SL+TTFTFPQSLGILGGKP  STYIVGVQDENA
Sbjct: 301 GQHDWSPILLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENA 360

Query: 361 FYLDPHEVQQVVNIDKDDLDADTSSYHCNVIRHIPLESMDPSLAIGFYCRDKDDFDDFCY 420
           FYLDPHEVQQVVNIDKDDL+ADTSSYHCNVIRHIPLES+DPSLAIGFYCRDKDDFD+FCY
Sbjct: 361 FYLDPHEVQQVVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDNFCY 420

Query: 421 RASKLADESDGAPLFTVAETHSTNSGRHGHALDNCSRLVEDDSDGVVHMPKEEEEAHEDD 480
           RASKLA+ESDGAPLFTVAETHSTNSGR   AL++ SRLVEDD+DG VHMP EEE AHEDD
Sbjct: 421 RASKLAEESDGAPLFTVAETHSTNSGRQSSALNDHSRLVEDDADGAVHMPNEEE-AHEDD 480

Query: 481 WQFL 483
           WQFL
Sbjct: 481 WQFL 483

BLAST of Csor.00g159870 vs. ExPASy TrEMBL
Match: A0A1S3B4R1 (Cysteine protease OS=Cucumis melo OX=3656 GN=LOC103485762 PE=3 SV=1)

HSP 1 Score: 850 bits (2195), Expect = 2.79e-309
Identity = 419/484 (86.57%), Postives = 443/484 (91.53%), Query Frame = 0

Query: 1   MGRGKDLNSTCSSASTTDSIDRTQKSVCPELGSKDHASSKASLWSSFLTSTLTIFEHHKE 60
           MGRGKDL STCSS +T D IDRT +SVC ELGSK+H SSKASLWS F +S  +I +HHK+
Sbjct: 1   MGRGKDLKSTCSSETTADVIDRTHRSVCSELGSKNHISSKASLWSGFFSSNFSICDHHKD 60

Query: 61  PSVSEKKTFHSRQNVWTTIRKVMTSGSMRRLQERILGSHRSGVYSSGGDIWLLGVCHKIS 120
            SVSEKK FHSR NVW T+RKVMTSGSMRR+QERILGS RSGVY+SGGDIWLLGVCHKIS
Sbjct: 61  SSVSEKKVFHSRHNVWATVRKVMTSGSMRRIQERILGSRRSGVYTSGGDIWLLGVCHKIS 120

Query: 121 QDQAPDDVTSN-GAAGFELDFSSRILITYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQ 180
           QD  PDD  S+ G AGFE DFSSRIL+TYRKGFNVIQDSKYTSDVNWGCMLRSSQMLV+Q
Sbjct: 121 QDHLPDDAASSTGVAGFEQDFSSRILMTYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVSQ 180

Query: 181 ALLFHRLGRSWRKTSRKPLDKEYVEILHLFGDSEKSAFSIHNILQAGRAYDLAAGSWVGP 240
           ALLFHRLGRSWRK S+KP DKEYVEILHLFGDSE SAFSIHN+LQAGRAYDLAAGSWVGP
Sbjct: 181 ALLFHRLGRSWRKPSQKPFDKEYVEILHLFGDSETSAFSIHNLLQAGRAYDLAAGSWVGP 240

Query: 241 YAMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSK 300
           YAMCRSWETLVR KRETP LQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSK
Sbjct: 241 YAMCRSWETLVRSKRETPILQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSK 300

Query: 301 GQLDWTPILLLVPLVLGLEKINSRYILSLKTTFTFPQSLGILGGKPSVSTYIVGVQDENA 360
           GQ DW+PILLLVPLVLGLEKIN RYI SL+TTFTFPQSLGILGGKP  STYIVGVQDENA
Sbjct: 301 GQHDWSPILLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENA 360

Query: 361 FYLDPHEVQQVVNIDKDDLDADTSSYHCNVIRHIPLESMDPSLAIGFYCRDKDDFDDFCY 420
           FYLDPHEVQQVVNIDKDDL+ADTSSYHCNVIRHIPLES+DPSLAIGFYCRDKDDFD+FCY
Sbjct: 361 FYLDPHEVQQVVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDNFCY 420

Query: 421 RASKLADESDGAPLFTVAETHSTNSGRHGHALDNCSRLVEDDSDGVVHMPKEEEEAHEDD 480
           RASKLA+ESDGAPLFTVAETHSTNSGR   AL++ SRLVEDD+DG VHMP EEE AHEDD
Sbjct: 421 RASKLAEESDGAPLFTVAETHSTNSGRQSSALNDHSRLVEDDADGAVHMPNEEE-AHEDD 480

Query: 481 WQFL 483
           WQFL
Sbjct: 481 WQFL 483

BLAST of Csor.00g159870 vs. ExPASy TrEMBL
Match: A0A0A0LK81 (Cysteine protease OS=Cucumis sativus OX=3659 GN=Csa_2G128640 PE=3 SV=1)

HSP 1 Score: 840 bits (2170), Expect = 1.80e-305
Identity = 414/484 (85.54%), Postives = 440/484 (90.91%), Query Frame = 0

Query: 1   MGRGKDLNSTCSSASTTDSIDRTQKSVCPELGSKDHASSKASLWSSFLTSTLTIFEHHKE 60
           MGRGKDL STCS     D+IDRT +SV PELGSK+H SSKAS WS F +S  +IFEHHK+
Sbjct: 1   MGRGKDLKSTCSPEPAADAIDRTHRSVYPELGSKNHISSKASSWSGFFSSNFSIFEHHKD 60

Query: 61  PSVSEKKTFHSRQNVWTTIRKVMTSGSMRRLQERILGSHRSGVYSSGGDIWLLGVCHKIS 120
            SV+EKK FH R NVW T+RKVMTSGSMRR+QER+LGS RSGVYSSGGDIWLLGVCHKIS
Sbjct: 61  SSVTEKKVFHPRHNVWATVRKVMTSGSMRRIQERLLGSRRSGVYSSGGDIWLLGVCHKIS 120

Query: 121 QDQAPDDVTSN-GAAGFELDFSSRILITYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQ 180
           QD  PDD  S+ G AG+E DFSSRIL+TYRKGF+VIQDSKYTSDVNWGCMLRSSQMLVAQ
Sbjct: 121 QDHPPDDAASSPGVAGYEQDFSSRILMTYRKGFHVIQDSKYTSDVNWGCMLRSSQMLVAQ 180

Query: 181 ALLFHRLGRSWRKTSRKPLDKEYVEILHLFGDSEKSAFSIHNILQAGRAYDLAAGSWVGP 240
           ALLFHRLGRSWRK S+KPLDKEYVEILHLFGDSE SAFSIHN+LQAGRAYDLAAGSWVGP
Sbjct: 181 ALLFHRLGRSWRKPSQKPLDKEYVEILHLFGDSETSAFSIHNLLQAGRAYDLAAGSWVGP 240

Query: 241 YAMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSK 300
           YAMCRSWETLVR KRETP LQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSK
Sbjct: 241 YAMCRSWETLVRSKRETPILQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSK 300

Query: 301 GQLDWTPILLLVPLVLGLEKINSRYILSLKTTFTFPQSLGILGGKPSVSTYIVGVQDENA 360
           GQ DW+PILLLVPLVLGLEKIN RYI SL+TTFTFPQSLGILGGKP  STYIVGVQDENA
Sbjct: 301 GQHDWSPILLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENA 360

Query: 361 FYLDPHEVQQVVNIDKDDLDADTSSYHCNVIRHIPLESMDPSLAIGFYCRDKDDFDDFCY 420
           FYLDPHEVQQVVNIDKDDL+ADTSSYHCNVIRHIPLES+DPSLAIGFYCRDKDDFD+FC+
Sbjct: 361 FYLDPHEVQQVVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDNFCH 420

Query: 421 RASKLADESDGAPLFTVAETHSTNSGRHGHALDNCSRLVEDDSDGVVHMPKEEEEAHEDD 480
           RASKLA+ESDGAPLFTVAETHSTN GR   AL++ SRLVEDD DGVVHMP EEE +HEDD
Sbjct: 421 RASKLAEESDGAPLFTVAETHSTNPGRQSSALNDHSRLVEDDGDGVVHMPNEEE-SHEDD 480

Query: 481 WQFL 483
           WQFL
Sbjct: 481 WQFL 483

BLAST of Csor.00g159870 vs. ExPASy TrEMBL
Match: A0A6J1ETR9 (Cysteine protease OS=Cucurbita moschata OX=3662 GN=LOC111436439 PE=3 SV=1)

HSP 1 Score: 828 bits (2138), Expect = 1.25e-300
Identity = 413/484 (85.33%), Postives = 443/484 (91.53%), Query Frame = 0

Query: 1   MGRGKDLNSTCSSASTTDSIDRTQKSVCPELGSKDHASSKASLWSSFLTSTLTIFEHHKE 60
           MGRGKDLNSTCSS S TD +DR+Q+S+CP LGS++H SSKASLWS F TST ++FEH+KE
Sbjct: 1   MGRGKDLNSTCSSESVTDIVDRSQQSICPTLGSRNHISSKASLWSGFFTSTFSVFEHNKE 60

Query: 61  PSVSEKKTFHSRQNVWTTIRKVMTSGSMRRLQERILGSHRSGVYSSGGDIWLLGVCHKIS 120
            SVSEKK  HSR NVWTT+R+VMTSGSMRR+QERILGS RSGVYSSGGDIWLLGVCHKIS
Sbjct: 61  SSVSEKKAVHSRHNVWTTVRRVMTSGSMRRIQERILGSRRSGVYSSGGDIWLLGVCHKIS 120

Query: 121 QDQAPDD-VTSNGAAGFELDFSSRILITYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQ 180
           QDQA DD VTS+  AGFELDFSSRIL+TYRKGFN IQDSKYTSDVNWGCMLRSSQMLVAQ
Sbjct: 121 QDQASDDAVTSDSVAGFELDFSSRILMTYRKGFNGIQDSKYTSDVNWGCMLRSSQMLVAQ 180

Query: 181 ALLFHRLGRSWRKTSRKPLDKEYVEILHLFGDSEKSAFSIHNILQAGRAYDLAAGSWVGP 240
           ALLFHRLGRSWRKTS+KPLDKEYVEILHLFGDSE SAFSIHN+LQAGR YDLAAGSWVGP
Sbjct: 181 ALLFHRLGRSWRKTSQKPLDKEYVEILHLFGDSETSAFSIHNLLQAGRPYDLAAGSWVGP 240

Query: 241 YAMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSK 300
           YA+CRSWETLVRLKRETP  QDQQLPMAIYIVSGDEDGERGGAPVL ID ASRHCF+FSK
Sbjct: 241 YAICRSWETLVRLKRETPTPQDQQLPMAIYIVSGDEDGERGGAPVLCIDVASRHCFQFSK 300

Query: 301 GQLDWTPILLLVPLVLGLEKINSRYILSLKTTFTFPQSLGILGGKPSVSTYIVGVQDENA 360
           GQLDWTPILLLVPLVLGLEKIN RYI SL+ TFTFPQSLGILGGK   STYIVGVQDENA
Sbjct: 301 GQLDWTPILLLVPLVLGLEKINPRYIPSLRATFTFPQSLGILGGKAGASTYIVGVQDENA 360

Query: 361 FYLDPHEVQQVVNIDKDDLDADTSSYHCNVIRHIPLESMDPSLAIGFYCRDKDDFDDFCY 420
           FYLDPHEVQQVVNIDKDDL+ADTSSYHCNVIRHIPLES+DPSLAIGFYCRDKDDFDDFCY
Sbjct: 361 FYLDPHEVQQVVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDDFCY 420

Query: 421 RASKLADESDGAPLFTVAETHSTNSGRHGHALDNCSRLVEDDSDGVVHMPKEEEEAHEDD 480
           RASKLA +S GAPLFTVAETHS+NS RHG+AL++ SRLV D++D  VH+P +EE A EDD
Sbjct: 421 RASKLAGDSYGAPLFTVAETHSSNSVRHGNALNDGSRLVVDNAD--VHVP-DEEGAQEDD 480

Query: 481 WQFL 483
           WQ L
Sbjct: 481 WQLL 481

BLAST of Csor.00g159870 vs. TAIR 10
Match: AT2G44140.1 (Peptidase family C54 protein )

HSP 1 Score: 547.0 bits (1408), Expect = 1.6e-155
Identity = 277/477 (58.07%), Postives = 354/477 (74.21%), Query Frame = 0

Query: 11  CSSASTTDSIDRTQKSVCPELGSKDHASSKASLWSSFLTSTLTIFEHHKEPSVSEKKTFH 70
           CSS+S +D+ D++   +  + G  D+  SK +LWS+  TS+ ++ + ++E S S  K   
Sbjct: 13  CSSSSKSDTHDKS--PLVSDSGPSDN-KSKFTLWSNVFTSSSSVSQPYRESSTSGHKQVC 72

Query: 71  SRQNVWTTI--RKVMTSGSMRRLQERILGSHRSGVYSSGGDIWLLGVCHKISQDQAPDDV 130
           + +N WT    R  M SG++RR QER+LG +R+G+ S+  D+WLLGVC+KIS D+   + 
Sbjct: 73  TTRNGWTAFVKRVSMASGAIRRFQERVLGPNRTGLPSTTSDVWLLGVCYKISADENSGET 132

Query: 131 -TSNGAAGFELDFSSRILITYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQALLFHRLG 190
            T    A  +LDFSS+IL+TYRKGF   +D+ YTSDVNWGCM+RSSQML AQALLFHRLG
Sbjct: 133 DTGTVLAALQLDFSSKILMTYRKGFEPFRDTTYTSDVNWGCMIRSSQMLFAQALLFHRLG 192

Query: 191 RSWRKTSRKPLDKEYVEILHLFGDSEKSAFSIHNILQAGRAYDLAAGSWVGPYAMCRSWE 250
           R+W K S  P ++EY+E L  FGDSE SAFSIHN++ AG +Y LAAGSWVGPYA+CR+WE
Sbjct: 193 RAWTKKSELP-EQEYLETLEPFGDSEPSAFSIHNLIIAGASYGLAAGSWVGPYAICRAWE 252

Query: 251 TLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSKGQLDWTPI 310
           +L   KR+  + ++Q LPMA++IVSG EDGERGGAP+L I+DA++ C EFSKGQ +WTPI
Sbjct: 253 SLACKKRKQTDSKNQTLPMAVHIVSGSEDGERGGAPILCIEDATKSCLEFSKGQSEWTPI 312

Query: 311 LLLVPLVLGLEKINSRYILSLKTTFTFPQSLGILGGKPSVSTYIVGVQDENAFYLDPHEV 370
           +LLVPLVLGL+ +N RYI SL  TFTFPQS+GILGGKP  STYIVGVQ++  FYLDPHEV
Sbjct: 313 ILLVPLVLGLDSVNPRYIPSLVATFTFPQSVGILGGKPGASTYIVGVQEDKGFYLDPHEV 372

Query: 371 QQVVNIDKDDLDADTSSYHCNVIRHIPLESMDPSLAIGFYCRDKDDFDDFCYRASKLADE 430
           QQVV ++K+  D DTSSYHCNV+R++PLES+DPSLA+GFYCRDKDDFDDFC RA KLA+E
Sbjct: 373 QQVVTVNKETPDVDTSSYHCNVLRYVPLESLDPSLALGFYCRDKDDFDDFCLRALKLAEE 432

Query: 431 SDGAPLFTVAETHST-NSGRHGHALDNCSRLVEDDSDGVVHMPKEEEEAHEDDWQFL 484
           S+GAPLFTV +TH+  N   +G A        +DDS          E+  EDDWQ L
Sbjct: 433 SNGAPLFTVTQTHTAINQSNYGFA--------DDDS----------EDEREDDWQML 467

BLAST of Csor.00g159870 vs. TAIR 10
Match: AT2G44140.2 (Peptidase family C54 protein )

HSP 1 Score: 527.3 bits (1357), Expect = 1.3e-149
Identity = 262/429 (61.07%), Postives = 324/429 (75.52%), Query Frame = 0

Query: 59  KEPSVSEKKTFHSRQNVWTTI--RKVMTSGSMRRLQERILGSHRSGVYSSGGDIWLLGVC 118
           +E S S  K   + +N WT    R  M SG++RR QER+LG +R+G+ S+  D+WLLGVC
Sbjct: 13  RESSTSGHKQVCTTRNGWTAFVKRVSMASGAIRRFQERVLGPNRTGLPSTTSDVWLLGVC 72

Query: 119 HKISQDQAPDDV-TSNGAAGFELDFSSRILITYRKGFNVIQDSKYTSDVNWGCMLRSSQM 178
           +KIS D+   +  T    A  +LDFSS+IL+TYRKGF   +D+ YTSDVNWGCM+RSSQM
Sbjct: 73  YKISADENSGETDTGTVLAALQLDFSSKILMTYRKGFEPFRDTTYTSDVNWGCMIRSSQM 132

Query: 179 LVAQALLFHRLGRSWRKTSRKPLDKEYVEILHLFGDSEKSAFSIHNILQAGRAYDLAAGS 238
           L AQALLFHRLGR+W K S  P ++EY+E L  FGDSE SAFSIHN++ AG +Y LAAGS
Sbjct: 133 LFAQALLFHRLGRAWTKKSELP-EQEYLETLEPFGDSEPSAFSIHNLIIAGASYGLAAGS 192

Query: 239 WVGPYAMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCF 298
           WVGPYA+CR+WE+L   KR+  + ++Q LPMA++IVSG EDGERGGAP+L I+DA++ C 
Sbjct: 193 WVGPYAICRAWESLACKKRKQTDSKNQTLPMAVHIVSGSEDGERGGAPILCIEDATKSCL 252

Query: 299 EFSKGQLDWTPILLLVPLVLGLEKINSRYILSLKTTFTFPQSLGILGGKPSVSTYIVGVQ 358
           EFSKGQ +WTPI+LLVPLVLGL+ +N RYI SL  TFTFPQS+GILGGKP  STYIVGVQ
Sbjct: 253 EFSKGQSEWTPIILLVPLVLGLDSVNPRYIPSLVATFTFPQSVGILGGKPGASTYIVGVQ 312

Query: 359 DENAFYLDPHEVQQVVNIDKDDLDADTSSYHCNVIRHIPLESMDPSLAIGFYCRDKDDFD 418
           ++  FYLDPHEVQQVV ++K+  D DTSSYHCNV+R++PLES+DPSLA+GFYCRDKDDFD
Sbjct: 313 EDKGFYLDPHEVQQVVTVNKETPDVDTSSYHCNVLRYVPLESLDPSLALGFYCRDKDDFD 372

Query: 419 DFCYRASKLADESDGAPLFTVAETHST-NSGRHGHALDNCSRLVEDDSDGVVHMPKEEEE 478
           DFC RA KLA+ES+GAPLFTV +TH+  N   +G A        +DDS          E+
Sbjct: 373 DFCLRALKLAEESNGAPLFTVTQTHTAINQSNYGFA--------DDDS----------ED 422

Query: 479 AHEDDWQFL 484
             EDDWQ L
Sbjct: 433 EREDDWQML 422

BLAST of Csor.00g159870 vs. TAIR 10
Match: AT3G59950.1 (Peptidase family C54 protein )

HSP 1 Score: 520.0 bits (1338), Expect = 2.0e-147
Identity = 262/479 (54.70%), Postives = 341/479 (71.19%), Query Frame = 0

Query: 9   STCSSASTTDSID-RTQKSVCPELGSKDHASSKASLWSSFLTSTLTIFEHHKEPSVSEKK 68
           S CSS+ST++  D  +  S+  +  S D+  S  +L S  + S+  + +  +E S S   
Sbjct: 11  SKCSSSSTSEKRDISSPTSLVSDSASSDN-KSNLTLCSDVVASSSPVSQLCREASTSGHN 70

Query: 69  TFHSRQNVWTTIRKV--MTSGSMRRLQERILGSHRSGVYSSGGDIWLLGVCHKISQDQAP 128
              +  + WT I K   M SG++RR Q+R+LG  R+G+ SS  +IWLLGVC+KIS+ ++ 
Sbjct: 71  PVCTTHSSWTVILKTASMASGAIRRFQDRVLGPSRTGISSSTSEIWLLGVCYKISEGESS 130

Query: 129 DDVTSNGA-AGFELDFSSRILITYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQALLFH 188
           ++  +    A F  DFSS IL+TYR+GF  I D+ YTSDVNWGCMLRS QML AQALLF 
Sbjct: 131 EEADAGRVLAAFRQDFSSLILMTYRRGFEPIGDTTYTSDVNWGCMLRSGQMLFAQALLFQ 190

Query: 189 RLGRSWRKTSRKPLDKEYVEILHLFGDSEKSAFSIHNILQAGRAYDLAAGSWVGPYAMCR 248
           RLGRSWRK   +P D++Y+EIL LFGD+E SAFSIHN++ AG +Y LAAGSWVGPYA+CR
Sbjct: 191 RLGRSWRKKDSEPADEKYLEILELFGDTEASAFSIHNLILAGESYGLAAGSWVGPYAVCR 250

Query: 249 SWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSKGQLDW 308
           SWE+L R  +E  + + +   MA++IVSG EDGERGGAP+L I+D ++ C EFS+G+ +W
Sbjct: 251 SWESLARKNKEETDDKHKSFSMAVHIVSGSEDGERGGAPILCIEDVTKTCLEFSEGETEW 310

Query: 309 TPILLLVPLVLGLEKINSRYILSLKTTFTFPQSLGILGGKPSVSTYIVGVQDENAFYLDP 368
            PILLLVPLVLGL+++N RYI SL  TFTFPQSLGILGGKP  STYIVGVQ++  FYLDP
Sbjct: 311 PPILLLVPLVLGLDRVNPRYIPSLIATFTFPQSLGILGGKPGASTYIVGVQEDKGFYLDP 370

Query: 369 HEVQQVVNIDKDDLDADTSSYHCNVIRHIPLESMDPSLAIGFYCRDKDDFDDFCYRASKL 428
           H+VQQVV + K++ D DTSSYHCN +R++PLES+DPSLA+GFYC+ KDDFDDFC RA+KL
Sbjct: 371 HDVQQVVTVKKENQDVDTSSYHCNTLRYVPLESLDPSLALGFYCQHKDDFDDFCIRATKL 430

Query: 429 ADESDGAPLFTVAETHSTNSGRHGHALDNCSRLVEDDSDGVVHMPKEEEEAHEDDWQFL 484
           A +S+GAPLFTV ++H  N         +C   + + S       +   E HEDDWQ L
Sbjct: 431 AGDSNGAPLFTVTQSHRRN---------DCG--IAETSSSTETSTEISGEEHEDDWQLL 477

BLAST of Csor.00g159870 vs. TAIR 10
Match: AT3G59950.2 (Peptidase family C54 protein )

HSP 1 Score: 328.2 bits (840), Expect = 1.1e-89
Identity = 166/317 (52.37%), Postives = 224/317 (70.66%), Query Frame = 0

Query: 9   STCSSASTTDSID-RTQKSVCPELGSKDHASSKASLWSSFLTSTLTIFEHHKEPSVSEKK 68
           S CSS+ST++  D  +  S+  +  S D+  S  +L S  + S+  + +  +E S S   
Sbjct: 11  SKCSSSSTSEKRDISSPTSLVSDSASSDN-KSNLTLCSDVVASSSPVSQLCREASTSGHN 70

Query: 69  TFHSRQNVWTTIRKV--MTSGSMRRLQERILGSHRSGVYSSGGDIWLLGVCHKISQDQAP 128
              +  + WT I K   M SG++RR Q+R+LG  R+G+ SS  +IWLLGVC+KIS+ ++ 
Sbjct: 71  PVCTTHSSWTVILKTASMASGAIRRFQDRVLGPSRTGISSSTSEIWLLGVCYKISEGESS 130

Query: 129 DDVTSNGA-AGFELDFSSRILITYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQALLFH 188
           ++  +    A F  DFSS IL+TYR+GF  I D+ YTSDVNWGCMLRS QML AQALLF 
Sbjct: 131 EEADAGRVLAAFRQDFSSLILMTYRRGFEPIGDTTYTSDVNWGCMLRSGQMLFAQALLFQ 190

Query: 189 RLGRSWRKTSRKPLDKEYVEILHLFGDSEKSAFSIHNILQAGRAYDLAAGSWVGPYAMCR 248
           RLGRSWRK   +P D++Y+EIL LFGD+E SAFSIHN++ AG +Y LAAGSWVGPYA+CR
Sbjct: 191 RLGRSWRKKDSEPADEKYLEILELFGDTEASAFSIHNLILAGESYGLAAGSWVGPYAVCR 250

Query: 249 SWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSKGQLDW 308
           SWE+L R  +E  + + +   MA++IVSG EDGERGGAP+L I+D ++ C EFS+G+ +W
Sbjct: 251 SWESLARKNKEETDDKHKSFSMAVHIVSGSEDGERGGAPILCIEDVTKTCLEFSEGETEW 310

Query: 309 TPILLLVPLVLGLEKIN 322
            PILLLVPLVLGL+++N
Sbjct: 311 PPILLLVPLVLGLDRVN 326

BLAST of Csor.00g159870 vs. TAIR 10
Match: AT3G59950.3 (Peptidase family C54 protein )

HSP 1 Score: 318.9 bits (816), Expect = 6.9e-87
Identity = 146/244 (59.84%), Postives = 191/244 (78.28%), Query Frame = 0

Query: 83  MTSGSMRRLQERILGSHRSGVYSSGGDIWLLGVCHKISQDQAPDDVTSNGA-AGFELDFS 142
           M SG++RR Q+R+LG  R+G+ SS  +IWLLGVC+KIS+ ++ ++  +    A F  DFS
Sbjct: 1   MASGAIRRFQDRVLGPSRTGISSSTSEIWLLGVCYKISEGESSEEADAGRVLAAFRQDFS 60

Query: 143 SRILITYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQALLFHRLGRSWRKTSRKPLDKE 202
           S IL+TYR+GF  I D+ YTSDVNWGCMLRS QML AQALLF RLGRSWRK   +P D++
Sbjct: 61  SLILMTYRRGFEPIGDTTYTSDVNWGCMLRSGQMLFAQALLFQRLGRSWRKKDSEPADEK 120

Query: 203 YVEILHLFGDSEKSAFSIHNILQAGRAYDLAAGSWVGPYAMCRSWETLVRLKRETPNLQD 262
           Y+EIL LFGD+E SAFSIHN++ AG +Y LAAGSWVGPYA+CRSWE+L R  +E  + + 
Sbjct: 121 YLEILELFGDTEASAFSIHNLILAGESYGLAAGSWVGPYAVCRSWESLARKNKEETDDKH 180

Query: 263 QQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSKGQLDWTPILLLVPLVLGLEKIN 322
           +   MA++IVSG EDGERGGAP+L I+D ++ C EFS+G+ +W PILLLVPLVLGL+++N
Sbjct: 181 KSFSMAVHIVSGSEDGERGGAPILCIEDVTKTCLEFSEGETEWPPILLLVPLVLGLDRVN 240

Query: 323 SRYI 326
            R++
Sbjct: 241 PRFV 244

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A2Q1V63.2e-17464.71Cysteine protease ATG4 OS=Medicago truncatula OX=3880 GN=ATG4 PE=3 SV=1[more]
Q8S9292.2e-15458.07Cysteine protease ATG4a OS=Arabidopsis thaliana OX=3702 GN=ATG4A PE=2 SV=1[more]
Q9M1Y02.9e-14654.70Cysteine protease ATG4b OS=Arabidopsis thaliana OX=3702 GN=ATG4B PE=1 SV=1[more]
Q2XPP41.2e-13956.79Cysteine protease ATG4B OS=Oryza sativa subsp. indica OX=39946 GN=ATG4B PE=1 SV=... [more]
Q7XPW83.4e-13956.57Cysteine protease ATG4B OS=Oryza sativa subsp. japonica OX=39947 GN=ATG4B PE=2 S... [more]
Match NameE-valueIdentityDescription
KAG6579717.10.0100.00Cysteine protease ATG4, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7017160.10.099.79Cysteine protease ATG4 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022928925.10.098.34cysteine protease ATG4-like [Cucurbita moschata] >XP_022928926.1 cysteine protea... [more]
XP_023551477.10.096.89cysteine protease ATG4-like [Cucurbita pepo subsp. pepo][more]
XP_038874853.10.090.29cysteine protease ATG4-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1ELN40.098.34Cysteine protease OS=Cucurbita moschata OX=3662 GN=LOC111435689 PE=3 SV=1[more]
A0A5A7SP822.79e-30986.57Cysteine protease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold340G00... [more]
A0A1S3B4R12.79e-30986.57Cysteine protease OS=Cucumis melo OX=3656 GN=LOC103485762 PE=3 SV=1[more]
A0A0A0LK811.80e-30585.54Cysteine protease OS=Cucumis sativus OX=3659 GN=Csa_2G128640 PE=3 SV=1[more]
A0A6J1ETR91.25e-30085.33Cysteine protease OS=Cucurbita moschata OX=3662 GN=LOC111436439 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G44140.11.6e-15558.07Peptidase family C54 protein [more]
AT2G44140.21.3e-14961.07Peptidase family C54 protein [more]
AT3G59950.12.0e-14754.70Peptidase family C54 protein [more]
AT3G59950.21.1e-8952.37Peptidase family C54 protein [more]
AT3G59950.36.9e-8759.84Peptidase family C54 protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005078Peptidase C54PFAMPF03416Peptidase_C54coord: 136..419
e-value: 2.3E-88
score: 296.2
IPR005078Peptidase C54PANTHERPTHR22624CYSTEINE PROTEASE ATG4coord: 26..483
NoneNo IPR availablePANTHERPTHR22624:SF54CYSTEINE PROTEASE ATG4Bcoord: 26..483
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 96..438

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g159870.m01Csor.00g159870.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006914 autophagy
biological_process GO:0006508 proteolysis
cellular_component GO:0005737 cytoplasm
molecular_function GO:0008234 cysteine-type peptidase activity