Csor.00g159000 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g159000
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	Delta(8)-fatty-acid desaturase
Location	Csor_Chr08: 5693106 .. 5694446 (+)
RNA-Seq Expression	Csor.00g159000
Synteny	Csor.00g159000

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codonsinglepolypeptidestop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGGCCGAGAAGTACATTTCGTCGGAGGAGCTTCTCAAGCACAACAAACCCGGAGATCTGTGGATTTCCATTCAGGGTAAGGTCTATAATGTCTCAGATTGGGCTAAAGTACATCCTGGTGGCGATGCGCCGTTTCTCCTCATGGCTGGTCATGATGTTACTGATGCATTTATCGCTTATCATCCTGGTACTGCTTGGGCGTATTTGGAACCCTTGTTTACTGGATTTCATCTCGAGGGTTTCCAGGTCTCGGAGGTTTCTAAGGATTATAGGCGGCTTGCTGCTGAGTTTTCAAAACAAGGTTTGTTCGAGAAGAAAGGGCATGTTACTATGTATTCTTTGATATCTGTTTTTATCATGATGTTCATTGTGGTTTATGGGGTTGTGAAATCTGAGAGTGTTTTGGTTCATTTGGGTTGTGCTATGATTTTGGGTATGCTTTGGATTCAAAGTGCTTATGTGGGTCATGATTCTGGACATTCTAATGTGATGTCGAGCCGTGGATTTAACAAATTGGCTCAAATTCTGTCTGGGAATTGCTTGACTGGGATTAGTATAGCCTGGTGGAAATGGACTCATAATGCTCATCATATCTCATGTAATAGCCTTGACCATGATCCAGATCTTCAACACATCCCTGTTTTTGCTGTTTCTGTTAATTTCTTCCGCTCTTTGACATCTCATTTCTATGGAAGGAAGCTCAAGTTCGATTCCTTGGCTAGATTCTTCGTTAGTTACCAGCATTTCACATTTTACCCTGTGATGTGTGTGGCTAGAGTGAATCTCTTTGTGCAAACATTATTGCTCTTGTTTTCCACTAGAAAAGTACCTGATAGAGCATTGAACATAATGGGGATTCTTGTGTTTTGGACATGGTTTCCTCTTCTAGTTTCGTATCTCCCTAATTGGCCTGAGAGATTCATGTTTGTTCTTGCAAGTTTCGCTGTTACTTCTCTACAGCATATCCAATTCTGCCTCAACCATTTCTCTGCAAATGTTTATGTTGGGAAGCCCATAGGGAATGATTGGTTTGAGAAGCAAACTAGTGGGACTTTGGATATCTCTTGTTCTTCGTGGATGGATTGGTTTTTTGGCGGATTACAGTTTCAGCTTGAACATCATTTGTTTCCAAGGATGCCCAGATGCCAATTGAGGAAGATATCCCCAATTGTTGTGGAGCTTTGCAAGAAACACAATCTTCCTTATAGAAGCTTGTCATTTTGGGATGCTAATGTCCGCACCATTAGAACTCTTAGAACAGCTGCTTTGCAGGCAAGAGATTTGACCAACCCTGTTCCTAAAAACTTGCTATGGGAAGCTGTGAACACCCATGGATGA

mRNA sequence

ATGGTGGCCGAGAAGTACATTTCGTCGGAGGAGCTTCTCAAGCACAACAAACCCGGAGATCTGTGGATTTCCATTCAGGGTAAGGTCTATAATGTCTCAGATTGGGCTAAAGTACATCCTGGTGGCGATGCGCCGTTTCTCCTCATGGCTGGTCATGATGTTACTGATGCATTTATCGCTTATCATCCTGGTACTGCTTGGGCGTATTTGGAACCCTTGTTTACTGGATTTCATCTCGAGGGTTTCCAGGTCTCGGAGGTTTCTAAGGATTATAGGCGGCTTGCTGCTGAGTTTTCAAAACAAGGTTTGTTCGAGAAGAAAGGGCATGTTACTATGTATTCTTTGATATCTGTTTTTATCATGATGTTCATTGTGGTTTATGGGGTTGTGAAATCTGAGAGTGTTTTGGTTCATTTGGGTTGTGCTATGATTTTGGGTATGCTTTGGATTCAAAGTGCTTATGTGGGTCATGATTCTGGACATTCTAATGTGATGTCGAGCCGTGGATTTAACAAATTGGCTCAAATTCTGTCTGGGAATTGCTTGACTGGGATTAGTATAGCCTGGTGGAAATGGACTCATAATGCTCATCATATCTCATGTAATAGCCTTGACCATGATCCAGATCTTCAACACATCCCTGTTTTTGCTGTTTCTGTTAATTTCTTCCGCTCTTTGACATCTCATTTCTATGGAAGGAAGCTCAAGTTCGATTCCTTGGCTAGATTCTTCGTTAGTTACCAGCATTTCACATTTTACCCTGTGATGTGTGTGGCTAGAGTGAATCTCTTTGTGCAAACATTATTGCTCTTGTTTTCCACTAGAAAAGTACCTGATAGAGCATTGAACATAATGGGGATTCTTGTGTTTTGGACATGGTTTCCTCTTCTAGTTTCGTATCTCCCTAATTGGCCTGAGAGATTCATGTTTGTTCTTGCAAGTTTCGCTGTTACTTCTCTACAGCATATCCAATTCTGCCTCAACCATTTCTCTGCAAATGTTTATGTTGGGAAGCCCATAGGGAATGATTGGTTTGAGAAGCAAACTAGTGGGACTTTGGATATCTCTTGTTCTTCGTGGATGGATTGGTTTTTTGGCGGATTACAGTTTCAGCTTGAACATCATTTGTTTCCAAGGATGCCCAGATGCCAATTGAGGAAGATATCCCCAATTGTTGTGGAGCTTTGCAAGAAACACAATCTTCCTTATAGAAGCTTGTCATTTTGGGATGCTAATGTCCGCACCATTAGAACTCTTAGAACAGCTGCTTTGCAGGCAAGAGATTTGACCAACCCTGTTCCTAAAAACTTGCTATGGGAAGCTGTGAACACCCATGGATGA

Coding sequence (CDS)

ATGGTGGCCGAGAAGTACATTTCGTCGGAGGAGCTTCTCAAGCACAACAAACCCGGAGATCTGTGGATTTCCATTCAGGGTAAGGTCTATAATGTCTCAGATTGGGCTAAAGTACATCCTGGTGGCGATGCGCCGTTTCTCCTCATGGCTGGTCATGATGTTACTGATGCATTTATCGCTTATCATCCTGGTACTGCTTGGGCGTATTTGGAACCCTTGTTTACTGGATTTCATCTCGAGGGTTTCCAGGTCTCGGAGGTTTCTAAGGATTATAGGCGGCTTGCTGCTGAGTTTTCAAAACAAGGTTTGTTCGAGAAGAAAGGGCATGTTACTATGTATTCTTTGATATCTGTTTTTATCATGATGTTCATTGTGGTTTATGGGGTTGTGAAATCTGAGAGTGTTTTGGTTCATTTGGGTTGTGCTATGATTTTGGGTATGCTTTGGATTCAAAGTGCTTATGTGGGTCATGATTCTGGACATTCTAATGTGATGTCGAGCCGTGGATTTAACAAATTGGCTCAAATTCTGTCTGGGAATTGCTTGACTGGGATTAGTATAGCCTGGTGGAAATGGACTCATAATGCTCATCATATCTCATGTAATAGCCTTGACCATGATCCAGATCTTCAACACATCCCTGTTTTTGCTGTTTCTGTTAATTTCTTCCGCTCTTTGACATCTCATTTCTATGGAAGGAAGCTCAAGTTCGATTCCTTGGCTAGATTCTTCGTTAGTTACCAGCATTTCACATTTTACCCTGTGATGTGTGTGGCTAGAGTGAATCTCTTTGTGCAAACATTATTGCTCTTGTTTTCCACTAGAAAAGTACCTGATAGAGCATTGAACATAATGGGGATTCTTGTGTTTTGGACATGGTTTCCTCTTCTAGTTTCGTATCTCCCTAATTGGCCTGAGAGATTCATGTTTGTTCTTGCAAGTTTCGCTGTTACTTCTCTACAGCATATCCAATTCTGCCTCAACCATTTCTCTGCAAATGTTTATGTTGGGAAGCCCATAGGGAATGATTGGTTTGAGAAGCAAACTAGTGGGACTTTGGATATCTCTTGTTCTTCGTGGATGGATTGGTTTTTTGGCGGATTACAGTTTCAGCTTGAACATCATTTGTTTCCAAGGATGCCCAGATGCCAATTGAGGAAGATATCCCCAATTGTTGTGGAGCTTTGCAAGAAACACAATCTTCCTTATAGAAGCTTGTCATTTTGGGATGCTAATGTCCGCACCATTAGAACTCTTAGAACAGCTGCTTTGCAGGCAAGAGATTTGACCAACCCTGTTCCTAAAAACTTGCTATGGGAAGCTGTGAACACCCATGGATGA

Protein sequence

MVAEKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKVHPGGDAPFLLMAGHDVTDAFIAYHPGTAWAYLEPLFTGFHLEGFQVSEVSKDYRRLAAEFSKQGLFEKKGHVTMYSLISVFIMMFIVVYGVVKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSVNFFRSLTSHFYGRKLKFDSLARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSYLPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTSGTLDISCSSWMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLRTAALQARDLTNPVPKNLLWEAVNTHG

Homology

[Close]

BLAST of Csor.00g159000 vs. ExPASy Swiss-Prot
Match: Q3EBF7 (Delta(8)-fatty-acid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=SLD2 PE=1 SV=1)

HSP 1 Score: 727.6 bits (1877), Expect = 8.3e-209
Identity = 333/443 (75.17%), Postives = 384/443 (86.68%), Query Frame = 0


Query: 4   EKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKVHPGGDAPFLLMAGHDVTDAFIAYHP 63
           ++Y++SE+L KHNKPGDLWISIQGKVY+VSDW K HPGG+A  L +AG DVTDAFIAYHP
Sbjct: 7   KRYVTSEDLKKHNKPGDLWISIQGKVYDVSDWVKSHPGGEAAILNLAGQDVTDAFIAYHP 66

Query: 64  GTAWAYLEPLFTGFHLEGFQVSEVSKDYRRLAAEFSKQGLFEKKGHVTMYSLISVFIMMF 123
           GTAW +LE L  G+H+    VS+VS+DYRRLAAEFSK+GLF+KKGHVT+Y+L  V +M+ 
Sbjct: 67  GTAWHHLEKLHNGYHVRDHHVSDVSRDYRRLAAEFSKRGLFDKKGHVTLYTLTCVGVMLA 126

Query: 124 IVVYGVVKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNCLT 183
            V+YGV+   S+  HL  A++LG+LWIQSAYVGHDSGH  V S++  NKL Q+LSGNCLT
Sbjct: 127 AVLYGVLACTSIWAHLISAVLLGLLWIQSAYVGHDSGHYTVTSTKPCNKLIQLLSGNCLT 186

Query: 184 GISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSVNFFRSLTSHFYGRKLKFDSLARF 243
           GISIAWWKWTHNAHHI+CNSLDHDPDLQHIP+FAVS  FF S+TS FYGRKL FD LARF
Sbjct: 187 GISIAWWKWTHNAHHIACNSLDHDPDLQHIPIFAVSTKFFNSMTSRFYGRKLTFDPLARF 246

Query: 244 FVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSYLPN 303
            +SYQH+TFYPVMCV R+NLF+QT LLLFS R VPDRALNI GILVFWTWFPLLVS+LPN
Sbjct: 247 LISYQHWTFYPVMCVGRINLFIQTFLLLFSKRHVPDRALNIAGILVFWTWFPLLVSFLPN 306

Query: 304 WPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTSGTLDISCSSWMDW 363
           W ERF+FV  SFAVT++QH+QFCLNHF+A+VY G P GNDWFEKQT+GTLDISC S+MDW
Sbjct: 307 WQERFIFVFVSFAVTAIQHVQFCLNHFAADVYTGPPNGNDWFEKQTAGTLDISCRSFMDW 366

Query: 364 FFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLRTAA 423
           FFGGLQFQLEHHLFPR+PRC LR +SP+V ELCKKHNLPYRSLS+W+ANV TIRTL+ AA
Sbjct: 367 FFGGLQFQLEHHLFPRLPRCHLRTVSPVVKELCKKHNLPYRSLSWWEANVWTIRTLKNAA 426

Query: 424 LQARDLTNPVPKNLLWEAVNTHG 447
           +QARD TNPV KNLLWEAVNTHG
Sbjct: 427 IQARDATNPVLKNLLWEAVNTHG 449

[Close]

BLAST of Csor.00g159000 vs. ExPASy Swiss-Prot
Match: Q9ZRP7 (Delta(8)-fatty-acid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=SLD1 PE=1 SV=1)

HSP 1 Score: 706.1 bits (1821), Expect = 2.6e-202
Identity = 323/443 (72.91%), Postives = 375/443 (84.65%), Query Frame = 0


Query: 4   EKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKVHPGGDAPFLLMAGHDVTDAFIAYHP 63
           +KYI++E+L KHNK GDLWI+IQGKVYNVSDW K HPGGD   L + G DVTDAFIA+HP
Sbjct: 7   KKYITNEDLKKHNKSGDLWIAIQGKVYNVSDWIKTHPGGDTVILNLVGQDVTDAFIAFHP 66

Query: 64  GTAWAYLEPLFTGFHLEGFQVSEVSKDYRRLAAEFSKQGLFEKKGHVTMYSLISVFIMMF 123
           GTAW +L+ LFTG+H+  FQVSEVS+DYRR+AAEF K GLFE KGHVT+Y+L  V  M  
Sbjct: 67  GTAWHHLDHLFTGYHIRDFQVSEVSRDYRRMAAEFRKLGLFENKGHVTLYTLAFVAAMFL 126

Query: 124 IVVYGVVKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNCLT 183
            V+YGV+   SV  H   A +LG+LWIQSAY+GHDSGH  +MS++ +N+ AQ+LSGNCLT
Sbjct: 127 GVLYGVLACTSVFAHQIAAALLGLLWIQSAYIGHDSGHYVIMSNKSYNRFAQLLSGNCLT 186

Query: 184 GISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSVNFFRSLTSHFYGRKLKFDSLARF 243
           GISIAWWKWTHNAHH++CNSLD+DPDLQHIPVFAVS  FF SLTS FY RKL FD +ARF
Sbjct: 187 GISIAWWKWTHNAHHLACNSLDYDPDLQHIPVFAVSTKFFSSLTSRFYDRKLTFDPVARF 246

Query: 244 FVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSYLPN 303
            VSYQHFT+YPVMC  R+NLF+QT LLLFS R+VPDRALN  GILVFWTWFPLLVS LPN
Sbjct: 247 LVSYQHFTYYPVMCFGRINLFIQTFLLLFSKREVPDRALNFAGILVFWTWFPLLVSCLPN 306

Query: 304 WPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTSGTLDISCSSWMDW 363
           WPERF FV  SF VT+LQHIQF LNHF+A+VYVG P G+DWFEKQ +GT+DISC S+MDW
Sbjct: 307 WPERFFFVFTSFTVTALQHIQFTLNHFAADVYVGPPTGSDWFEKQAAGTIDISCRSYMDW 366

Query: 364 FFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLRTAA 423
           FFGGLQFQLEHHLFPR+PRC LRK+SP+V ELCKKHNLPYRS+S+++ANV TI TL+TAA
Sbjct: 367 FFGGLQFQLEHHLFPRLPRCHLRKVSPVVQELCKKHNLPYRSMSWFEANVLTINTLKTAA 426

Query: 424 LQARDLTNPVPKNLLWEAVNTHG 447
            QARD+ NPV KNL+WEA+NTHG
Sbjct: 427 YQARDVANPVVKNLVWEALNTHG 449

[Close]

BLAST of Csor.00g159000 vs. ExPASy Swiss-Prot
Match: Q9FR82 (Delta(8)-fatty-acid desaturase OS=Borago officinalis OX=13363 GN=sld1 PE=1 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 3.9e-198
Identity = 325/443 (73.36%), Postives = 376/443 (84.88%), Query Frame = 0


Query: 4   EKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKVHPGGDAPFLLMAGHDVTDAFIAYHP 63
           +KYIS  EL KHN+ GD+WISIQGKVYNV+DW K HPGGD P + +AG D TDAFIAYHP
Sbjct: 5   KKYISVGELEKHNQLGDVWISIQGKVYNVTDWIKKHPGGDVPIMNLAGQDATDAFIAYHP 64

Query: 64  GTAWAYLEPLFTGFHLEGFQVSEVSKDYRRLAAEFSKQGLFEKKGHVTMYSLISVFIMMF 123
           GTAW  LE LFTG+HLE + VSE+SKDYR+LA+EFSK GLFEKKGH  +Y L  + +++ 
Sbjct: 65  GTAWKNLENLFTGYHLEDYLVSEISKDYRKLASEFSKAGLFEKKGHTVIYCLSFIALLLC 124

Query: 124 IVVYGVVKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNCLT 183
             VYGV+ S S+ VH+    +LGM +IQ+AY+GHDSGH  +MSS+G+NK AQ+L+GNCLT
Sbjct: 125 GCVYGVLCSNSLWVHMLSGAMLGMCFIQAAYLGHDSGHYTMMSSKGYNKFAQVLNGNCLT 184

Query: 184 GISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSVNFFRSLTSHFYGRKLKFDSLARF 243
           GISIAWWKWTHNAHHI+CNSLD+DPDLQH+PVFAV  +FF+SLTS FYGR+L FD L+RF
Sbjct: 185 GISIAWWKWTHNAHHIACNSLDYDPDLQHLPVFAVPSSFFKSLTSRFYGRELTFDGLSRF 244

Query: 244 FVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSYLPN 303
            VSYQHFT Y VM   R+NL+VQT LLLFSTRKVPDRALNI+GILV+WTWFP LVS LPN
Sbjct: 245 LVSYQHFTIYLVMIFGRINLYVQTFLLLFSTRKVPDRALNIIGILVYWTWFPYLVSCLPN 304

Query: 304 WPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTSGTLDISCSSWMDW 363
           W ER +FVL  F+VT+LQHIQF LNHF+A+VYVG P G +WFEKQ +GT+DISCSSWMDW
Sbjct: 305 WNERVLFVLTCFSVTALQHIQFTLNHFAADVYVGPPTGTNWFEKQAAGTIDISCSSWMDW 364

Query: 364 FFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLRTAA 423
           FFGGLQFQLEHHLFPRMPRCQLR ISPIV + CKKHNLPYRSLSF+DANV TI+TLRTAA
Sbjct: 365 FFGGLQFQLEHHLFPRMPRCQLRNISPIVQDYCKKHNLPYRSLSFFDANVATIKTLRTAA 424

Query: 424 LQARDLTNPVPKNLLWEAVNTHG 447
           LQARDLT  VP+NLLWEA NTHG
Sbjct: 425 LQARDLT-VVPQNLLWEAFNTHG 446

[Close]

BLAST of Csor.00g159000 vs. ExPASy Swiss-Prot
Match: Q43469 (Delta(8)-fatty-acid desaturase OS=Helianthus annuus OX=4232 GN=sld1 PE=1 SV=1)

HSP 1 Score: 688.0 bits (1774), Expect = 7.3e-197
Identity = 311/443 (70.20%), Postives = 374/443 (84.42%), Query Frame = 0


Query: 4   EKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKVHPGGDAPFLLMAGHDVTDAFIAYHP 63
           +KYI+S+EL KHN P DLWISI GKVYNV++WAK HPGGDAP + +AG DVTDAFIA+HP
Sbjct: 16  KKYITSKELKKHNNPNDLWISILGKVYNVTEWAKEHPGGDAPLINLAGQDVTDAFIAFHP 75

Query: 64  GTAWAYLEPLFTGFHLEGFQVSEVSKDYRRLAAEFSKQGLFEKKGHVTMYSLISVFIMMF 123
           GTAW +L+ LFTG+HL+ +QVS++S+DYR+LA+EF+K G+FEKKGH  +YSL  V +++ 
Sbjct: 76  GTAWKHLDKLFTGYHLKDYQVSDISRDYRKLASEFAKAGMFEKKGHGVIYSLCFVSLLLS 135

Query: 124 IVVYGVVKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNCLT 183
             VYGV+ S S  +H+    ILG+ W+Q AY+GHD+GH  +M++RG+NK A I  GNC+T
Sbjct: 136 ACVYGVLYSGSFWIHMLSGAILGLAWMQIAYLGHDAGHYQMMATRGWNKFAGIFIGNCIT 195

Query: 184 GISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSVNFFRSLTSHFYGRKLKFDSLARF 243
           GISIAWWKWTHNAHHI+CNSLD+DPDLQH+P+ AVS   F S+TS FYGR+L FD LARF
Sbjct: 196 GISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSSKLFNSITSVFYGRQLTFDPLARF 255

Query: 244 FVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSYLPN 303
           FVSYQH+ +YP+MCVARVNL++QT+LLL S RK+PDR LNI+G L+FWTWFPLLVS LPN
Sbjct: 256 FVSYQHYLYYPIMCVARVNLYLQTILLLISKRKIPDRGLNILGTLIFWTWFPLLVSRLPN 315

Query: 304 WPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTSGTLDISCSSWMDW 363
           WPER  FVL SF VT +QHIQF LNHFS +VYVG P G++WFEKQT GT+DI+CSSWMDW
Sbjct: 316 WPERVAFVLVSFCVTGIQHIQFTLNHFSGDVYVGPPKGDNWFEKQTRGTIDIACSSWMDW 375

Query: 364 FFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLRTAA 423
           FFGGLQFQLEHHLFPR+PRC LR ISPI  ELCKK+NLPY SLSF+DANV T++TLRTAA
Sbjct: 376 FFGGLQFQLEHHLFPRLPRCHLRSISPICRELCKKYNLPYVSLSFYDANVTTLKTLRTAA 435

Query: 424 LQARDLTNPVPKNLLWEAVNTHG 447
           LQARDLTNP P+NL WEA NTHG
Sbjct: 436 LQARDLTNPAPQNLAWEAFNTHG 458

[Close]

BLAST of Csor.00g159000 vs. ExPASy Swiss-Prot
Match: O04353 (Acyl-lipid (9-3)-desaturase OS=Borago officinalis OX=13363 PE=1 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 2.1e-175
Identity = 279/443 (62.98%), Postives = 347/443 (78.33%), Query Frame = 0


Query: 4   EKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKVHPGGDAPFLLMAGHDVTDAFIAYHP 63
           +KYI+S+EL  H+KPGDLWISIQGK Y+VSDW K HPGG  P   +AG +VTDAF+A+HP
Sbjct: 6   KKYITSDELKNHDKPGDLWISIQGKAYDVSDWVKDHPGGSFPLKSLAGQEVTDAFVAFHP 65

Query: 64  GTAWAYLEPLFTGFHLEGFQVSEVSKDYRRLAAEFSKQGLFEKKGHVTMYSLISVFIMMF 123
            + W  L+  FTG++L+ + VSEVSKDYR+L  EFSK GL++KKGH+   +L  + ++  
Sbjct: 66  ASTWKNLDKFFTGYYLKDYSVSEVSKDYRKLVFEFSKMGLYDKKGHIMFATLCFIAMLFA 125

Query: 124 IVVYGVVKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNCLT 183
           + VYGV+  E VLVHL    ++G LWIQS ++GHD+GH  V+S    NK   I + NCL+
Sbjct: 126 MSVYGVLFCEGVLVHLFSGCLMGFLWIQSGWIGHDAGHYMVVSDSRLNKFMGIFAANCLS 185

Query: 184 GISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSVNFFRSLTSHFYGRKLKFDSLARF 243
           GISI WWKW HNAHHI+CNSL++DPDLQ+IP   VS  FF SLTSHFY ++L FDSL+RF
Sbjct: 186 GISIGWWKWNHNAHHIACNSLEYDPDLQYIPFLVVSSKFFGSLTSHFYEKRLTFDSLSRF 245

Query: 244 FVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSYLPN 303
           FVSYQH+TFYP+MC AR+N++VQ+L++L + R V  RA  ++G LVF  W+PLLVS LPN
Sbjct: 246 FVSYQHWTFYPIMCAARLNMYVQSLIMLLTKRNVSYRAHELLGCLVFSIWYPLLVSCLPN 305

Query: 304 WPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTSGTLDISCSSWMDW 363
           W ER MFV+AS +VT +Q +QF LNHFS++VYVGKP GN+WFEKQT GTLDISC  WMDW
Sbjct: 306 WGERIMFVIASLSVTGMQQVQFSLNHFSSSVYVGKPKGNNWFEKQTDGTLDISCPPWMDW 365

Query: 364 FFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLRTAA 423
           F GGLQFQ+EHHLFP+MPRC LRKISP V+ELCKKHNLPY   SF  AN  T+RTLR  A
Sbjct: 366 FHGGLQFQIEHHLFPKMPRCNLRKISPYVIELCKKHNLPYNYASFSKANEMTLRTLRNTA 425

Query: 424 LQARDLTNPVPKNLLWEAVNTHG 447
           LQARD+T P+PKNL+WEA++THG
Sbjct: 426 LQARDITKPLPKNLVWEALHTHG 448

[Close]

BLAST of Csor.00g159000 vs. NCBI nr
Match: XP_022964381.1 (delta(8)-fatty-acid desaturase 2-like [Cucurbita moschata] >KAG6593635.1 Delta(8)-fatty-acid desaturase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025977.1 Delta(8)-fatty-acid desaturase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 921 bits (2381), Expect = 0.0
Identity = 446/446 (100.00%), Postives = 446/446 (100.00%), Query Frame = 0


Query: 1   MVAEKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKVHPGGDAPFLLMAGHDVTDAFIA 60
           MVAEKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKVHPGGDAPFLLMAGHDVTDAFIA
Sbjct: 1   MVAEKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKVHPGGDAPFLLMAGHDVTDAFIA 60

Query: 61  YHPGTAWAYLEPLFTGFHLEGFQVSEVSKDYRRLAAEFSKQGLFEKKGHVTMYSLISVFI 120
           YHPGTAWAYLEPLFTGFHLEGFQVSEVSKDYRRLAAEFSKQGLFEKKGHVTMYSLISVFI
Sbjct: 61  YHPGTAWAYLEPLFTGFHLEGFQVSEVSKDYRRLAAEFSKQGLFEKKGHVTMYSLISVFI 120

Query: 121 MMFIVVYGVVKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGN 180
           MMFIVVYGVVKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGN
Sbjct: 121 MMFIVVYGVVKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGN 180

Query: 181 CLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSVNFFRSLTSHFYGRKLKFDSL 240
           CLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSVNFFRSLTSHFYGRKLKFDSL
Sbjct: 181 CLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSVNFFRSLTSHFYGRKLKFDSL 240

Query: 241 ARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSY 300
           ARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSY
Sbjct: 241 ARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSY 300

Query: 301 LPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTSGTLDISCSSW 360
           LPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTSGTLDISCSSW
Sbjct: 301 LPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTSGTLDISCSSW 360

Query: 361 MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLR 420
           MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLR
Sbjct: 361 MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLR 420

Query: 421 TAALQARDLTNPVPKNLLWEAVNTHG 446
           TAALQARDLTNPVPKNLLWEAVNTHG
Sbjct: 421 TAALQARDLTNPVPKNLLWEAVNTHG 446

[Close]

BLAST of Csor.00g159000 vs. NCBI nr
Match: XP_023000173.1 (delta(8)-fatty-acid desaturase 2-like [Cucurbita maxima] >XP_023514253.1 delta(8)-fatty-acid desaturase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 920 bits (2378), Expect = 0.0
Identity = 445/446 (99.78%), Postives = 446/446 (100.00%), Query Frame = 0


Query: 1   MVAEKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKVHPGGDAPFLLMAGHDVTDAFIA 60
           MVAEKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKVHPGGDAPFLLMAGHDVTDAFIA
Sbjct: 1   MVAEKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKVHPGGDAPFLLMAGHDVTDAFIA 60

Query: 61  YHPGTAWAYLEPLFTGFHLEGFQVSEVSKDYRRLAAEFSKQGLFEKKGHVTMYSLISVFI 120
           YHPGTAWAYLEPLFTGFHLEGFQVSEVSKDYRRLAAEFSKQGLFEKKGHVTMYSLISVFI
Sbjct: 61  YHPGTAWAYLEPLFTGFHLEGFQVSEVSKDYRRLAAEFSKQGLFEKKGHVTMYSLISVFI 120

Query: 121 MMFIVVYGVVKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGN 180
           M+FIVVYGVVKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGN
Sbjct: 121 MLFIVVYGVVKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGN 180

Query: 181 CLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSVNFFRSLTSHFYGRKLKFDSL 240
           CLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSVNFFRSLTSHFYGRKLKFDSL
Sbjct: 181 CLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSVNFFRSLTSHFYGRKLKFDSL 240

Query: 241 ARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSY 300
           ARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSY
Sbjct: 241 ARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSY 300

Query: 301 LPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTSGTLDISCSSW 360
           LPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTSGTLDISCSSW
Sbjct: 301 LPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTSGTLDISCSSW 360

Query: 361 MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLR 420
           MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLR
Sbjct: 361 MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLR 420

Query: 421 TAALQARDLTNPVPKNLLWEAVNTHG 446
           TAALQARDLTNPVPKNLLWEAVNTHG
Sbjct: 421 TAALQARDLTNPVPKNLLWEAVNTHG 446

[Close]

BLAST of Csor.00g159000 vs. NCBI nr
Match: XP_008458161.1 (PREDICTED: delta(8)-fatty-acid desaturase 2 [Cucumis melo] >TYK14028.1 delta(8)-fatty-acid desaturase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 875 bits (2262), Expect = 0.0
Identity = 419/445 (94.16%), Postives = 433/445 (97.30%), Query Frame = 0


Query: 2   VAEKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKVHPGGDAPFLLMAGHDVTDAFIAY 61
           V +KYI+SEELLKHNKPGDLWISIQGKVYNV++WAKVHPGGD PFL +AGHDVTDAFIAY
Sbjct: 3   VEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFIAY 62

Query: 62  HPGTAWAYLEPLFTGFHLEGFQVSEVSKDYRRLAAEFSKQGLFEKKGHVTMYSLISVFIM 121
           HPGTAWAYL+ LFTGFHL+ FQVSE+SKDYR+LA+EFSKQGLFEKKGHV MYSLISV +M
Sbjct: 63  HPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVAVM 122

Query: 122 MFIVVYGVVKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNC 181
           MF+VVYGV+KSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNC
Sbjct: 123 MFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNC 182

Query: 182 LTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSVNFFRSLTSHFYGRKLKFDSLA 241
           LTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVS  FFR+LTSHFYGRKLKFDSLA
Sbjct: 183 LTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDSLA 242

Query: 242 RFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSYL 301
           RFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSYL
Sbjct: 243 RFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSYL 302

Query: 302 PNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTSGTLDISCSSWM 361
           PNWPER MFVLASFAV SLQHIQFCLNHFSANVYVGKP GNDWFEKQTSGTLDISCSSWM
Sbjct: 303 PNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSSWM 362

Query: 362 DWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLRT 421
           DWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLRT
Sbjct: 363 DWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLRT 422

Query: 422 AALQARDLTNPVPKNLLWEAVNTHG 446
           AALQARDLTNPVPKNLLWEAVNTHG
Sbjct: 423 AALQARDLTNPVPKNLLWEAVNTHG 447

[Close]

BLAST of Csor.00g159000 vs. NCBI nr
Match: XP_031743128.1 (delta(8)-fatty-acid desaturase 2 [Cucumis sativus] >ANN11751.1 delta8-sphingolipid desaturase [Cucumis sativus] >KGN45699.1 hypothetical protein Csa_005537 [Cucumis sativus])

HSP 1 Score: 870 bits (2249), Expect = 0.0
Identity = 415/445 (93.26%), Postives = 433/445 (97.30%), Query Frame = 0


Query: 2   VAEKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKVHPGGDAPFLLMAGHDVTDAFIAY 61
           V +KYI+SEELLKHNKPGDLWISIQGKVYNV++WAK HPGGD PF+ +AGHDVTDAFIAY
Sbjct: 3   VEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKEHPGGDTPFINLAGHDVTDAFIAY 62

Query: 62  HPGTAWAYLEPLFTGFHLEGFQVSEVSKDYRRLAAEFSKQGLFEKKGHVTMYSLISVFIM 121
           HPGTAWAYL+ LFTGFHL+ FQVSE+SKDYR+LA+EFSKQGLFEKKGHVTMYSLISV +M
Sbjct: 63  HPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVTMYSLISVAVM 122

Query: 122 MFIVVYGVVKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNC 181
           MF+VVYGV+KSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNC
Sbjct: 123 MFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNC 182

Query: 182 LTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSVNFFRSLTSHFYGRKLKFDSLA 241
           LTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVS +FFR+LTSHFYGRKL+FDSLA
Sbjct: 183 LTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTSFFRTLTSHFYGRKLEFDSLA 242

Query: 242 RFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSYL 301
           RFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSYL
Sbjct: 243 RFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSYL 302

Query: 302 PNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTSGTLDISCSSWM 361
           PNWPER MFVLASFAV SLQHIQFCLNHFSANVYVGKP GNDWFEKQ SGTLDISCS+WM
Sbjct: 303 PNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQASGTLDISCSTWM 362

Query: 362 DWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLRT 421
           DWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLRT
Sbjct: 363 DWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLRT 422

Query: 422 AALQARDLTNPVPKNLLWEAVNTHG 446
           AALQARDLTNPVPKNLLWEAVNTHG
Sbjct: 423 AALQARDLTNPVPKNLLWEAVNTHG 447

[Close]

BLAST of Csor.00g159000 vs. NCBI nr
Match: XP_023006577.1 (delta(8)-fatty-acid desaturase 2-like [Cucurbita maxima] >XP_023006578.1 delta(8)-fatty-acid desaturase 2-like [Cucurbita maxima])

HSP 1 Score: 868 bits (2242), Expect = 0.0
Identity = 420/447 (93.96%), Postives = 431/447 (96.42%), Query Frame = 0


Query: 1   MVAEK-YISSEELLKHNKPGDLWISIQGKVYNVSDWAKVHPGGDAPFLLMAGHDVTDAFI 60
           M AEK YI+SEELLKHNKPGDLWISIQGKVYNV+DWAK HPGGD PFL +AGHDVTDAFI
Sbjct: 1   MEAEKKYITSEELLKHNKPGDLWISIQGKVYNVTDWAKEHPGGDTPFLNLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLEPLFTGFHLEGFQVSEVSKDYRRLAAEFSKQGLFEKKGHVTMYSLISVF 120
           AYHPGTAWAYL+ LFTGFHLE F VSEVSKDYRRLA+EFSKQGLFEKKGHVTMYSLISV 
Sbjct: 61  AYHPGTAWAYLDRLFTGFHLEDFHVSEVSKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120

Query: 121 IMMFIVVYGVVKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180
           IM+F+VVYGVVKSESVLVHLG AMILGMLWIQSAYVGHDSGHS VMSSRGFNKLAQILSG
Sbjct: 121 IMLFLVVYGVVKSESVLVHLGSAMILGMLWIQSAYVGHDSGHSTVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSVNFFRSLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVS +FF+SLTSHFYGR LKFDS
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTSFFKSLTSHFYGRTLKFDS 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300
           LARFF+SYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS
Sbjct: 241 LARFFISYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 301 YLPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTSGTLDISCSS 360
           YLPNWPER MFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQT GTLDISCSS
Sbjct: 301 YLPNWPERVMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTKGTLDISCSS 360

Query: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL 420
           WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTI TL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTINTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 446
           RTAALQARDLTNPVP+NLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPQNLLWEAVNTHG 447

[Close]

BLAST of Csor.00g159000 vs. ExPASy TrEMBL
Match: A0A6J1HIR5 (delta(8)-fatty-acid desaturase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111464415 PE=4 SV=1)

HSP 1 Score: 921 bits (2381), Expect = 0.0
Identity = 446/446 (100.00%), Postives = 446/446 (100.00%), Query Frame = 0


Query: 1   MVAEKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKVHPGGDAPFLLMAGHDVTDAFIA 60
           MVAEKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKVHPGGDAPFLLMAGHDVTDAFIA
Sbjct: 1   MVAEKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKVHPGGDAPFLLMAGHDVTDAFIA 60

Query: 61  YHPGTAWAYLEPLFTGFHLEGFQVSEVSKDYRRLAAEFSKQGLFEKKGHVTMYSLISVFI 120
           YHPGTAWAYLEPLFTGFHLEGFQVSEVSKDYRRLAAEFSKQGLFEKKGHVTMYSLISVFI
Sbjct: 61  YHPGTAWAYLEPLFTGFHLEGFQVSEVSKDYRRLAAEFSKQGLFEKKGHVTMYSLISVFI 120

Query: 121 MMFIVVYGVVKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGN 180
           MMFIVVYGVVKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGN
Sbjct: 121 MMFIVVYGVVKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGN 180

Query: 181 CLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSVNFFRSLTSHFYGRKLKFDSL 240
           CLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSVNFFRSLTSHFYGRKLKFDSL
Sbjct: 181 CLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSVNFFRSLTSHFYGRKLKFDSL 240

Query: 241 ARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSY 300
           ARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSY
Sbjct: 241 ARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSY 300

Query: 301 LPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTSGTLDISCSSW 360
           LPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTSGTLDISCSSW
Sbjct: 301 LPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTSGTLDISCSSW 360

Query: 361 MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLR 420
           MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLR
Sbjct: 361 MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLR 420

Query: 421 TAALQARDLTNPVPKNLLWEAVNTHG 446
           TAALQARDLTNPVPKNLLWEAVNTHG
Sbjct: 421 TAALQARDLTNPVPKNLLWEAVNTHG 446

[Close]

BLAST of Csor.00g159000 vs. ExPASy TrEMBL
Match: A0A6J1KLW2 (delta(8)-fatty-acid desaturase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111494458 PE=4 SV=1)

HSP 1 Score: 920 bits (2378), Expect = 0.0
Identity = 445/446 (99.78%), Postives = 446/446 (100.00%), Query Frame = 0


Query: 1   MVAEKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKVHPGGDAPFLLMAGHDVTDAFIA 60
           MVAEKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKVHPGGDAPFLLMAGHDVTDAFIA
Sbjct: 1   MVAEKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKVHPGGDAPFLLMAGHDVTDAFIA 60

Query: 61  YHPGTAWAYLEPLFTGFHLEGFQVSEVSKDYRRLAAEFSKQGLFEKKGHVTMYSLISVFI 120
           YHPGTAWAYLEPLFTGFHLEGFQVSEVSKDYRRLAAEFSKQGLFEKKGHVTMYSLISVFI
Sbjct: 61  YHPGTAWAYLEPLFTGFHLEGFQVSEVSKDYRRLAAEFSKQGLFEKKGHVTMYSLISVFI 120

Query: 121 MMFIVVYGVVKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGN 180
           M+FIVVYGVVKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGN
Sbjct: 121 MLFIVVYGVVKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGN 180

Query: 181 CLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSVNFFRSLTSHFYGRKLKFDSL 240
           CLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSVNFFRSLTSHFYGRKLKFDSL
Sbjct: 181 CLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSVNFFRSLTSHFYGRKLKFDSL 240

Query: 241 ARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSY 300
           ARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSY
Sbjct: 241 ARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSY 300

Query: 301 LPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTSGTLDISCSSW 360
           LPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTSGTLDISCSSW
Sbjct: 301 LPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTSGTLDISCSSW 360

Query: 361 MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLR 420
           MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLR
Sbjct: 361 MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLR 420

Query: 421 TAALQARDLTNPVPKNLLWEAVNTHG 446
           TAALQARDLTNPVPKNLLWEAVNTHG
Sbjct: 421 TAALQARDLTNPVPKNLLWEAVNTHG 446

[Close]

BLAST of Csor.00g159000 vs. ExPASy TrEMBL
Match: A0A5D3CS55 (Delta(8)-fatty-acid desaturase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold268G00250 PE=4 SV=1)

HSP 1 Score: 875 bits (2262), Expect = 0.0
Identity = 419/445 (94.16%), Postives = 433/445 (97.30%), Query Frame = 0


Query: 2   VAEKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKVHPGGDAPFLLMAGHDVTDAFIAY 61
           V +KYI+SEELLKHNKPGDLWISIQGKVYNV++WAKVHPGGD PFL +AGHDVTDAFIAY
Sbjct: 3   VEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFIAY 62

Query: 62  HPGTAWAYLEPLFTGFHLEGFQVSEVSKDYRRLAAEFSKQGLFEKKGHVTMYSLISVFIM 121
           HPGTAWAYL+ LFTGFHL+ FQVSE+SKDYR+LA+EFSKQGLFEKKGHV MYSLISV +M
Sbjct: 63  HPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVAVM 122

Query: 122 MFIVVYGVVKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNC 181
           MF+VVYGV+KSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNC
Sbjct: 123 MFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNC 182

Query: 182 LTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSVNFFRSLTSHFYGRKLKFDSLA 241
           LTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVS  FFR+LTSHFYGRKLKFDSLA
Sbjct: 183 LTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDSLA 242

Query: 242 RFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSYL 301
           RFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSYL
Sbjct: 243 RFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSYL 302

Query: 302 PNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTSGTLDISCSSWM 361
           PNWPER MFVLASFAV SLQHIQFCLNHFSANVYVGKP GNDWFEKQTSGTLDISCSSWM
Sbjct: 303 PNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSSWM 362

Query: 362 DWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLRT 421
           DWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLRT
Sbjct: 363 DWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLRT 422

Query: 422 AALQARDLTNPVPKNLLWEAVNTHG 446
           AALQARDLTNPVPKNLLWEAVNTHG
Sbjct: 423 AALQARDLTNPVPKNLLWEAVNTHG 447

[Close]

BLAST of Csor.00g159000 vs. ExPASy TrEMBL
Match: A0A1S3C7C0 (delta(8)-fatty-acid desaturase 2 OS=Cucumis melo OX=3656 GN=LOC103497683 PE=4 SV=1)

HSP 1 Score: 875 bits (2262), Expect = 0.0
Identity = 419/445 (94.16%), Postives = 433/445 (97.30%), Query Frame = 0


Query: 2   VAEKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKVHPGGDAPFLLMAGHDVTDAFIAY 61
           V +KYI+SEELLKHNKPGDLWISIQGKVYNV++WAKVHPGGD PFL +AGHDVTDAFIAY
Sbjct: 3   VEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFIAY 62

Query: 62  HPGTAWAYLEPLFTGFHLEGFQVSEVSKDYRRLAAEFSKQGLFEKKGHVTMYSLISVFIM 121
           HPGTAWAYL+ LFTGFHL+ FQVSE+SKDYR+LA+EFSKQGLFEKKGHV MYSLISV +M
Sbjct: 63  HPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVAVM 122

Query: 122 MFIVVYGVVKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNC 181
           MF+VVYGV+KSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNC
Sbjct: 123 MFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNC 182

Query: 182 LTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSVNFFRSLTSHFYGRKLKFDSLA 241
           LTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVS  FFR+LTSHFYGRKLKFDSLA
Sbjct: 183 LTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDSLA 242

Query: 242 RFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSYL 301
           RFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSYL
Sbjct: 243 RFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSYL 302

Query: 302 PNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTSGTLDISCSSWM 361
           PNWPER MFVLASFAV SLQHIQFCLNHFSANVYVGKP GNDWFEKQTSGTLDISCSSWM
Sbjct: 303 PNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSSWM 362

Query: 362 DWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLRT 421
           DWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLRT
Sbjct: 363 DWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLRT 422

Query: 422 AALQARDLTNPVPKNLLWEAVNTHG 446
           AALQARDLTNPVPKNLLWEAVNTHG
Sbjct: 423 AALQARDLTNPVPKNLLWEAVNTHG 447

[Close]

BLAST of Csor.00g159000 vs. ExPASy TrEMBL
Match: A0A0A0KA29 (Delta8-sphingolipid desaturase OS=Cucumis sativus OX=3659 GN=Csa_6G006850 PE=4 SV=1)

HSP 1 Score: 870 bits (2249), Expect = 0.0
Identity = 415/445 (93.26%), Postives = 433/445 (97.30%), Query Frame = 0


Query: 2   VAEKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKVHPGGDAPFLLMAGHDVTDAFIAY 61
           V +KYI+SEELLKHNKPGDLWISIQGKVYNV++WAK HPGGD PF+ +AGHDVTDAFIAY
Sbjct: 3   VEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKEHPGGDTPFINLAGHDVTDAFIAY 62

Query: 62  HPGTAWAYLEPLFTGFHLEGFQVSEVSKDYRRLAAEFSKQGLFEKKGHVTMYSLISVFIM 121
           HPGTAWAYL+ LFTGFHL+ FQVSE+SKDYR+LA+EFSKQGLFEKKGHVTMYSLISV +M
Sbjct: 63  HPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVTMYSLISVAVM 122

Query: 122 MFIVVYGVVKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNC 181
           MF+VVYGV+KSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNC
Sbjct: 123 MFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNC 182

Query: 182 LTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSVNFFRSLTSHFYGRKLKFDSLA 241
           LTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVS +FFR+LTSHFYGRKL+FDSLA
Sbjct: 183 LTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTSFFRTLTSHFYGRKLEFDSLA 242

Query: 242 RFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSYL 301
           RFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSYL
Sbjct: 243 RFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSYL 302

Query: 302 PNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTSGTLDISCSSWM 361
           PNWPER MFVLASFAV SLQHIQFCLNHFSANVYVGKP GNDWFEKQ SGTLDISCS+WM
Sbjct: 303 PNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQASGTLDISCSTWM 362

Query: 362 DWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLRT 421
           DWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLRT
Sbjct: 363 DWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLRT 422

Query: 422 AALQARDLTNPVPKNLLWEAVNTHG 446
           AALQARDLTNPVPKNLLWEAVNTHG
Sbjct: 423 AALQARDLTNPVPKNLLWEAVNTHG 447

[Close]

BLAST of Csor.00g159000 vs. TAIR 10
Match: AT2G46210.1 (Fatty acid/sphingolipid desaturase )

HSP 1 Score: 727.6 bits (1877), Expect = 5.9e-210
Identity = 333/443 (75.17%), Postives = 384/443 (86.68%), Query Frame = 0


Query: 4   EKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKVHPGGDAPFLLMAGHDVTDAFIAYHP 63
           ++Y++SE+L KHNKPGDLWISIQGKVY+VSDW K HPGG+A  L +AG DVTDAFIAYHP
Sbjct: 7   KRYVTSEDLKKHNKPGDLWISIQGKVYDVSDWVKSHPGGEAAILNLAGQDVTDAFIAYHP 66

Query: 64  GTAWAYLEPLFTGFHLEGFQVSEVSKDYRRLAAEFSKQGLFEKKGHVTMYSLISVFIMMF 123
           GTAW +LE L  G+H+    VS+VS+DYRRLAAEFSK+GLF+KKGHVT+Y+L  V +M+ 
Sbjct: 67  GTAWHHLEKLHNGYHVRDHHVSDVSRDYRRLAAEFSKRGLFDKKGHVTLYTLTCVGVMLA 126

Query: 124 IVVYGVVKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNCLT 183
            V+YGV+   S+  HL  A++LG+LWIQSAYVGHDSGH  V S++  NKL Q+LSGNCLT
Sbjct: 127 AVLYGVLACTSIWAHLISAVLLGLLWIQSAYVGHDSGHYTVTSTKPCNKLIQLLSGNCLT 186

Query: 184 GISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSVNFFRSLTSHFYGRKLKFDSLARF 243
           GISIAWWKWTHNAHHI+CNSLDHDPDLQHIP+FAVS  FF S+TS FYGRKL FD LARF
Sbjct: 187 GISIAWWKWTHNAHHIACNSLDHDPDLQHIPIFAVSTKFFNSMTSRFYGRKLTFDPLARF 246

Query: 244 FVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSYLPN 303
            +SYQH+TFYPVMCV R+NLF+QT LLLFS R VPDRALNI GILVFWTWFPLLVS+LPN
Sbjct: 247 LISYQHWTFYPVMCVGRINLFIQTFLLLFSKRHVPDRALNIAGILVFWTWFPLLVSFLPN 306

Query: 304 WPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTSGTLDISCSSWMDW 363
           W ERF+FV  SFAVT++QH+QFCLNHF+A+VY G P GNDWFEKQT+GTLDISC S+MDW
Sbjct: 307 WQERFIFVFVSFAVTAIQHVQFCLNHFAADVYTGPPNGNDWFEKQTAGTLDISCRSFMDW 366

Query: 364 FFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLRTAA 423
           FFGGLQFQLEHHLFPR+PRC LR +SP+V ELCKKHNLPYRSLS+W+ANV TIRTL+ AA
Sbjct: 367 FFGGLQFQLEHHLFPRLPRCHLRTVSPVVKELCKKHNLPYRSLSWWEANVWTIRTLKNAA 426

Query: 424 LQARDLTNPVPKNLLWEAVNTHG 447
           +QARD TNPV KNLLWEAVNTHG
Sbjct: 427 IQARDATNPVLKNLLWEAVNTHG 449

[Close]

BLAST of Csor.00g159000 vs. TAIR 10
Match: AT3G61580.1 (Fatty acid/sphingolipid desaturase )

HSP 1 Score: 706.1 bits (1821), Expect = 1.8e-203
Identity = 323/443 (72.91%), Postives = 375/443 (84.65%), Query Frame = 0


Query: 4   EKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKVHPGGDAPFLLMAGHDVTDAFIAYHP 63
           +KYI++E+L KHNK GDLWI+IQGKVYNVSDW K HPGGD   L + G DVTDAFIA+HP
Sbjct: 7   KKYITNEDLKKHNKSGDLWIAIQGKVYNVSDWIKTHPGGDTVILNLVGQDVTDAFIAFHP 66

Query: 64  GTAWAYLEPLFTGFHLEGFQVSEVSKDYRRLAAEFSKQGLFEKKGHVTMYSLISVFIMMF 123
           GTAW +L+ LFTG+H+  FQVSEVS+DYRR+AAEF K GLFE KGHVT+Y+L  V  M  
Sbjct: 67  GTAWHHLDHLFTGYHIRDFQVSEVSRDYRRMAAEFRKLGLFENKGHVTLYTLAFVAAMFL 126

Query: 124 IVVYGVVKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNCLT 183
            V+YGV+   SV  H   A +LG+LWIQSAY+GHDSGH  +MS++ +N+ AQ+LSGNCLT
Sbjct: 127 GVLYGVLACTSVFAHQIAAALLGLLWIQSAYIGHDSGHYVIMSNKSYNRFAQLLSGNCLT 186

Query: 184 GISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSVNFFRSLTSHFYGRKLKFDSLARF 243
           GISIAWWKWTHNAHH++CNSLD+DPDLQHIPVFAVS  FF SLTS FY RKL FD +ARF
Sbjct: 187 GISIAWWKWTHNAHHLACNSLDYDPDLQHIPVFAVSTKFFSSLTSRFYDRKLTFDPVARF 246

Query: 244 FVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSYLPN 303
            VSYQHFT+YPVMC  R+NLF+QT LLLFS R+VPDRALN  GILVFWTWFPLLVS LPN
Sbjct: 247 LVSYQHFTYYPVMCFGRINLFIQTFLLLFSKREVPDRALNFAGILVFWTWFPLLVSCLPN 306

Query: 304 WPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTSGTLDISCSSWMDW 363
           WPERF FV  SF VT+LQHIQF LNHF+A+VYVG P G+DWFEKQ +GT+DISC S+MDW
Sbjct: 307 WPERFFFVFTSFTVTALQHIQFTLNHFAADVYVGPPTGSDWFEKQAAGTIDISCRSYMDW 366

Query: 364 FFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLRTAA 423
           FFGGLQFQLEHHLFPR+PRC LRK+SP+V ELCKKHNLPYRS+S+++ANV TI TL+TAA
Sbjct: 367 FFGGLQFQLEHHLFPRLPRCHLRKVSPVVQELCKKHNLPYRSMSWFEANVLTINTLKTAA 426

Query: 424 LQARDLTNPVPKNLLWEAVNTHG 447
            QARD+ NPV KNL+WEA+NTHG
Sbjct: 427 YQARDVANPVVKNLVWEALNTHG 449

[Close]

BLAST of Csor.00g159000 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 67.8 bits (164), Expect = 2.5e-11
Identity = 46/126 (36.51%), Postives = 66/126 (52.38%), Query Frame = 0


Query: 5   KYISSEELLKHNKPGDLWISIQGKVYNVSDWAKVHPGGDAPFLLMAGHDVTDAF-IAYHP 64
           K  S EE   HNK  D W+ I GKVY+VS +   HPGGD   L +AG D TD F  A H 
Sbjct: 6   KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHS 65

Query: 65  GTAWAYLEPLFTGFHLEGFQVSEVS--KDYRRLAAEFSKQGLFE--KKGHVTMYSLISVF 124
             A   +E  F G  L+   + E+   K Y++   + S Q LF+  K+  V   S+I++ 
Sbjct: 66  KDARELMEKYFIG-ELDESSLPEIPELKIYKKDQPQDSVQKLFDLTKQYWVVPVSIITIS 125

Query: 125 IMMFIV 126
           + + ++
Sbjct: 126 VAVSVL 130

[Close]

BLAST of Csor.00g159000 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 60.8 bits (146), Expect = 3.1e-09
Identity = 31/73 (42.47%), Postives = 40/73 (54.79%), Query Frame = 0


Query: 5  KYISSEELLKHNKPGDLWISIQGKVYNVSDWAKVHPGGDAPFLLMAGHDVTDAF-IAYHP 64
          K +S EE+ KHNK  D W+ I GKVY+V+ +   HPGGD   L   G D T+ F    H 
Sbjct: 6  KVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFEDVGHS 65

Query: 65 GTAWAYLEPLFTG 77
           TA   ++  F G
Sbjct: 66 DTARDMMDKYFIG 78

[Close]

BLAST of Csor.00g159000 vs. TAIR 10
Match: AT1G60660.1 (cytochrome B5-like protein )

HSP 1 Score: 58.2 bits (139), Expect = 2.0e-08
Identity = 30/77 (38.96%), Postives = 41/77 (53.25%), Query Frame = 0


Query: 1   MVAEKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKVHPGGDAPFLLMAGHDVTDAFIA 60
           +V  K  S  E+  HNK  D WI I+ KVY+++ + + HPGGDA  L  AG D TD F  
Sbjct: 43  VVRPKSYSKSEVAVHNKRNDCWIIIKDKVYDITSYVEEHPGGDA-ILDHAGDDSTDGFFG 102

Query: 61  -YHPGTAWAYLEPLFTG 77
             H    +  +E  + G
Sbjct: 103 PQHATRVFDMIEDFYIG 118

The following BLAST results are available for this feature:

BLAST of Csor.00g159000 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3EBF7	8.3e-209	75.17	Delta(8)-fatty-acid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=SLD2 PE=1 SV...	[more]
Q9ZRP7	2.6e-202	72.91	Delta(8)-fatty-acid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=SLD1 PE=1 SV...	[more]
Q9FR82	3.9e-198	73.36	Delta(8)-fatty-acid desaturase OS=Borago officinalis OX=13363 GN=sld1 PE=1 SV=1	[more]
Q43469	7.3e-197	70.20	Delta(8)-fatty-acid desaturase OS=Helianthus annuus OX=4232 GN=sld1 PE=1 SV=1	[more]
O04353	2.1e-175	62.98	Acyl-lipid (9-3)-desaturase OS=Borago officinalis OX=13363 PE=1 SV=1	[more]

BLAST of Csor.00g159000 vs. NCBI nr


Match Name	Identity	Description
XP_022964381.1	100.00	delta(8)-fatty-acid desaturase 2-like [Cucurbita moschata] >KAG6593635.1 Delta(8...	[more]
XP_023000173.1	99.78	delta(8)-fatty-acid desaturase 2-like [Cucurbita maxima] >XP_023514253.1 delta(8...	[more]
XP_008458161.1	94.16	PREDICTED: delta(8)-fatty-acid desaturase 2 [Cucumis melo] >TYK14028.1 delta(8)-...	[more]
XP_031743128.1	93.26	delta(8)-fatty-acid desaturase 2 [Cucumis sativus] >ANN11751.1 delta8-sphingolip...	[more]
XP_023006577.1	93.96	delta(8)-fatty-acid desaturase 2-like [Cucurbita maxima] >XP_023006578.1 delta(8...	[more]

BLAST of Csor.00g159000 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1HIR5	100.00	delta(8)-fatty-acid desaturase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111464...	[more]
A0A6J1KLW2	99.78	delta(8)-fatty-acid desaturase 2-like OS=Cucurbita maxima OX=3661 GN=LOC11149445...	[more]
A0A5D3CS55	94.16	Delta(8)-fatty-acid desaturase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3C7C0	94.16	delta(8)-fatty-acid desaturase 2 OS=Cucumis melo OX=3656 GN=LOC103497683 PE=4 SV...	[more]
A0A0A0KA29	93.26	Delta8-sphingolipid desaturase OS=Cucumis sativus OX=3659 GN=Csa_6G006850 PE=4 S...	[more]

BLAST of Csor.00g159000 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G46210.1	5.9e-210	75.17	Fatty acid/sphingolipid desaturase	[more]
AT3G61580.1	1.8e-203	72.91	Fatty acid/sphingolipid desaturase	[more]
AT1G26340.1	2.5e-11	36.51	cytochrome B5 isoform A	[more]
AT5G53560.1	3.1e-09	42.47	cytochrome B5 isoform E	[more]
AT1G60660.1	2.0e-08	38.96	cytochrome B5-like protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 7..79 e-value: 6.3E-19 score: 78.9
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 9..76 e-value: 1.1E-19 score: 70.3
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 4..88 score: 21.125031
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 1..76 e-value: 3.0E-19 score: 70.6
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 5..85
IPR012171	Fatty acid desaturase	PIRSF	PIRSF015921	FA_sphingolip_des	coord: 1..433 e-value: 2.1E-127 score: 423.7
IPR005804	Fatty acid desaturase domain	PFAM	PF00487	FA_desaturase	coord: 136..405 e-value: 8.7E-36 score: 124.1
None	No IPR available	PANTHER	PTHR19353	FATTY ACID DESATURASE 2	coord: 4..445
None	No IPR available	PANTHER	PTHR19353:SF28	DELTA(8)-FATTY-ACID DESATURASE 2	coord: 4..445
None	No IPR available	CDD	cd03506	Delta6-FADS-like	coord: 139..401 e-value: 5.53569E-63 score: 200.561

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g159000.m01	Csor.00g159000.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016717	oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water
molecular_function	GO:0016491	oxidoreductase activity