Csor.00g158120 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g158120
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	Germin-like protein
Location	Csor_Chr08: 5043083 .. 5043703 (+)
RNA-Seq Expression	Csor.00g158120
Synteny	Csor.00g158120

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codonsinglepolypeptidestop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTCACTCCCTATTTGTCATTGCCCTCCTCTTCTCTCTTTCCAATGCAGCCGTCCAAGACTTCTGCGTTGCTGATCTAAAAGGTCCACAAGGGCCAGCAGGCTACGCCTGCAAGAGCGCCGTGACTGCAGATGACTTCGTGTACTCAGGCCTAAGCGGTGCTGGAAACATAACAAGTCTAATCAAAGCTGCAGTCACCCCAGCATTTGTGGAGCAGTATCCAGGCGTGAATGGACTCGGTCTCTCGGCTGCTCGGTTAGACTTGCTTCCTGGTGGTGTTATACCACTTCACACACACCCAGGAGCTTCAGAATTGCTGATTGTGACTCAGGGTATCATTCAGGCTGGTTTTATCTCTTCCGCTAATGCTGTCTTCGTGAAGACTCTTAAAAAGGGAGATCTCATGATATTCCCACAAGGGTTGCTACATTTTCAGATTAATGCTGGTGGTGTGACTGCCGTTGGTTATCCTGTATTTAGCAGCCCAAGCCCTGGTCTCCAAATCACTGACTTTGCTCTGTTTGCCAACGACTTGAGTACTGACTTGGTGTCGAAGACCACTTTCCTCGACCCCGCTCTGGTGAAAAAGTTCAAAGCTATTCTCGGCGGGAGTGGTTAG

mRNA sequence

ATGTTTCACTCCCTATTTGTCATTGCCCTCCTCTTCTCTCTTTCCAATGCAGCCGTCCAAGACTTCTGCGTTGCTGATCTAAAAGGTCCACAAGGGCCAGCAGGCTACGCCTGCAAGAGCGCCGTGACTGCAGATGACTTCGTGTACTCAGGCCTAAGCGGTGCTGGAAACATAACAAGTCTAATCAAAGCTGCAGTCACCCCAGCATTTGTGGAGCAGTATCCAGGCGTGAATGGACTCGGTCTCTCGGCTGCTCGGTTAGACTTGCTTCCTGGTGGTGTTATACCACTTCACACACACCCAGGAGCTTCAGAATTGCTGATTGTGACTCAGGGTATCATTCAGGCTGGTTTTATCTCTTCCGCTAATGCTGTCTTCGTGAAGACTCTTAAAAAGGGAGATCTCATGATATTCCCACAAGGGTTGCTACATTTTCAGATTAATGCTGGTGGTGTGACTGCCGTTGGTTATCCTGTATTTAGCAGCCCAAGCCCTGGTCTCCAAATCACTGACTTTGCTCTGTTTGCCAACGACTTGAGTACTGACTTGGTGTCGAAGACCACTTTCCTCGACCCCGCTCTGGTGAAAAAGTTCAAAGCTATTCTCGGCGGGAGTGGTTAG

Coding sequence (CDS)

ATGTTTCACTCCCTATTTGTCATTGCCCTCCTCTTCTCTCTTTCCAATGCAGCCGTCCAAGACTTCTGCGTTGCTGATCTAAAAGGTCCACAAGGGCCAGCAGGCTACGCCTGCAAGAGCGCCGTGACTGCAGATGACTTCGTGTACTCAGGCCTAAGCGGTGCTGGAAACATAACAAGTCTAATCAAAGCTGCAGTCACCCCAGCATTTGTGGAGCAGTATCCAGGCGTGAATGGACTCGGTCTCTCGGCTGCTCGGTTAGACTTGCTTCCTGGTGGTGTTATACCACTTCACACACACCCAGGAGCTTCAGAATTGCTGATTGTGACTCAGGGTATCATTCAGGCTGGTTTTATCTCTTCCGCTAATGCTGTCTTCGTGAAGACTCTTAAAAAGGGAGATCTCATGATATTCCCACAAGGGTTGCTACATTTTCAGATTAATGCTGGTGGTGTGACTGCCGTTGGTTATCCTGTATTTAGCAGCCCAAGCCCTGGTCTCCAAATCACTGACTTTGCTCTGTTTGCCAACGACTTGAGTACTGACTTGGTGTCGAAGACCACTTTCCTCGACCCCGCTCTGGTGAAAAAGTTCAAAGCTATTCTCGGCGGGAGTGGTTAG

Protein sequence

MFHSLFVIALLFSLSNAAVQDFCVADLKGPQGPAGYACKSAVTADDFVYSGLSGAGNITSLIKAAVTPAFVEQYPGVNGLGLSAARLDLLPGGVIPLHTHPGASELLIVTQGIIQAGFISSANAVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPVFSSPSPGLQITDFALFANDLSTDLVSKTTFLDPALVKKFKAILGGSG

Homology

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BLAST of Csor.00g158120 vs. ExPASy Swiss-Prot
Match: Q9ZRA4 (Auxin-binding protein ABP19a OS=Prunus persica OX=3760 GN=ABP19A PE=3 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 4.3e-75
Identity = 138/207 (66.67%), Postives = 166/207 (80.19%), Query Frame = 0


Query: 1   MFHSLFVIALLFSLSNAAVQDFCVADLKGPQGPAGYACK--SAVTADDFVYSGLSGAGNI 60
           +F   F   LL S S+A+VQDFCVAD K P GPAGY+CK  + VT +DFVYSGL  AGN 
Sbjct: 2   IFPIFFTFFLLLSSSHASVQDFCVADYKAPDGPAGYSCKKPAKVTINDFVYSGLGIAGNT 61

Query: 61  TSLIKAAVTPAFVEQYPGVNGLGLSAARLDLLPGGVIPLHTHPGASELLIVTQGIIQAGF 120
           T++IKAAVTPAF  Q+PGVNGLG+S ARLDL PGGVIP HTHPGASE+L+V QG I AGF
Sbjct: 62  TNIIKAAVTPAFAAQFPGVNGLGISLARLDLGPGGVIPFHTHPGASEVLLVVQGTIIAGF 121

Query: 121 ISSANAVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPVFSSPSPGLQITDFALFAND 180
           ++S N  ++KTLKKGD+M+FPQGLLHFQ+N GG  A+ +P FSSPSPGLQI DFALF ND
Sbjct: 122 VASDNTPYLKTLKKGDIMVFPQGLLHFQVNGGGTPALAFPSFSSPSPGLQILDFALFKND 181

Query: 181 LSTDLVSKTTFLDPALVKKFKAILGGS 206
           L T+L+++TTFLD A +KK K +LGG+
Sbjct: 182 LPTELIAQTTFLDAAQIKKLKGVLGGT 208

[Close]

BLAST of Csor.00g158120 vs. ExPASy Swiss-Prot
Match: O04012 (Auxin-binding protein ABP19b OS=Prunus persica OX=3760 GN=ABP19B PE=3 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 8.9e-73
Identity = 132/207 (63.77%), Postives = 163/207 (78.74%), Query Frame = 0


Query: 1   MFHSLFVIALLFSLSNAAVQDFCVADLKGPQGPAGYACK--SAVTADDFVYSGLSGAGNI 60
           +F   F   LL S S+A+VQDFCVAD K P GPAGY+CK  + VT +DFVYSGL  AGN 
Sbjct: 2   IFPIFFTFFLLLSTSHASVQDFCVADYKAPDGPAGYSCKKPAIVTVNDFVYSGLGIAGNT 61

Query: 61  TSLIKAAVTPAFVEQYPGVNGLGLSAARLDLLPGGVIPLHTHPGASELLIVTQGIIQAGF 120
           T++ KAAVTPAF  Q+PGVNGLG+S ARLDL PGGV+P HTHPGASE+L+V QG I AGF
Sbjct: 62  TNIFKAAVTPAFAAQFPGVNGLGISLARLDLGPGGVVPFHTHPGASEVLLVVQGTIIAGF 121

Query: 121 ISSANAVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPVFSSPSPGLQITDFALFAND 180
           ++S N  ++KTLKKGD+++FPQGLLHFQ+N G   A+ +P FSSPSPGLQI DFALF ND
Sbjct: 122 VASDNTPYLKTLKKGDIIVFPQGLLHFQVNGGDTPAIAFPSFSSPSPGLQIVDFALFKND 181

Query: 181 LSTDLVSKTTFLDPALVKKFKAILGGS 206
           L+T+L+++TT LD   +KK K +LGG+
Sbjct: 182 LATELIAQTTLLDAPQIKKLKGVLGGT 208

[Close]

BLAST of Csor.00g158120 vs. ExPASy Swiss-Prot
Match: L8BRS3 (Germin-like protein OS=Morus alba OX=3498 PE=1 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 1.9e-70
Identity = 130/203 (64.04%), Postives = 162/203 (79.80%), Query Frame = 0


Query: 6   FVIALLFSLSNAAVQDFCVADLKGPQGPAGYACK--SAVTADDFVYSGLSGAGNITSLIK 65
           F+ +LLFS S+ A+QDFCVAD   PQGPAGY+CK  + VT D+FVYSGL   GN T+LIK
Sbjct: 7   FLFSLLFSSSHGAIQDFCVADYSAPQGPAGYSCKNPAKVTVDNFVYSGLGITGNTTNLIK 66

Query: 66  AAVTPAFVEQYPGVNGLGLSAARLDLLPGGVIPLHTHPGASELLIVTQGIIQAGFISSAN 125
           AAVT AF  Q+PGVNGLG+S AR DL PGGVIP HTHPGASE++IV +G + A F+SS N
Sbjct: 67  AAVTTAFDNQFPGVNGLGISLARPDLAPGGVIPFHTHPGASEIIIVIEGSLCAAFVSSDN 126

Query: 126 AVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPVFSSPSPGLQITDFALFANDLSTDL 185
            V++K+LKKGD MIFP GLLHFQ+NAG   A+ +  F+SP+PGLQ+ D+ALF NDL+T+L
Sbjct: 127 KVYLKSLKKGDTMIFPSGLLHFQLNAGKNNALFFVAFNSPNPGLQLVDYALFGNDLATEL 186

Query: 186 VSKTTFLDPALVKKFKAILGGSG 207
           V+  +FLDPA +K+ KA+LGGSG
Sbjct: 187 VAAASFLDPAEIKRLKAVLGGSG 209

[Close]

BLAST of Csor.00g158120 vs. ExPASy Swiss-Prot
Match: O04011 (Auxin-binding protein ABP20 OS=Prunus persica OX=3760 GN=ABP20 PE=2 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 3.2e-70
Identity = 130/207 (62.80%), Postives = 164/207 (79.23%), Query Frame = 0


Query: 1   MFHSLFVIALLFSLSNAAVQDFCVADLKGPQGPAGYACK--SAVTADDFVYSGLSGAGNI 60
           +F  LF   LL S SNAAVQDFCVADL  P+GPAG++CK  ++V  +DFV+SGL  AGN 
Sbjct: 7   IFPILFTFFLLLSSSNAAVQDFCVADLAAPEGPAGFSCKKPASVKVNDFVFSGLGIAGNT 66

Query: 61  TSLIKAAVTPAFVEQYPGVNGLGLSAARLDLLPGGVIPLHTHPGASELLIVTQGIIQAGF 120
           +++IKAAVTPAFV Q+PGVNGLG+S ARLDL  GGV+P HTHPGASE+LIV QG I AGF
Sbjct: 67  SNIIKAAVTPAFVAQFPGVNGLGISIARLDLAVGGVVPFHTHPGASEVLIVAQGTICAGF 126

Query: 121 ISSANAVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPVFSSPSPGLQITDFALFAND 180
           ++S N  +++TL+KGD+M+FPQGLLHFQ+N G   A+ +  F S SPGLQI DFALF ND
Sbjct: 127 VASDNTPYLQTLEKGDIMVFPQGLLHFQVNGGEAPALAFASFGSASPGLQILDFALFKND 186

Query: 181 LSTDLVSKTTFLDPALVKKFKAILGGS 206
           L T+++++TTFLD A +KK K +LGG+
Sbjct: 187 LPTEVIAQTTFLDAAQIKKLKGVLGGT 213

[Close]

BLAST of Csor.00g158120 vs. ExPASy Swiss-Prot
Match: P94040 (Germin-like protein subfamily 3 member 1 OS=Arabidopsis thaliana OX=3702 GN=GLP1 PE=1 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 1.7e-68
Identity = 133/208 (63.94%), Postives = 160/208 (76.92%), Query Frame = 0


Query: 1   MFHSLFVIALLFSLSNAAVQDFCVADLKGPQGPAGYAC--KSAVTADDFVYSGLSGAGNI 60
           M  ++F+++LLF+LSNA+VQDFCVA+LK  + PAGY C     V A DFV+SGL   GN 
Sbjct: 1   MLRTIFLLSLLFALSNASVQDFCVANLKRAETPAGYPCIRPIHVKATDFVFSGLGTPGNT 60

Query: 61  TSLIKAAVTPAFVEQYPGVNGLGLSAARLDLLPGGVIPLHTHPGASELLIVTQGIIQAGF 120
           T++I AAVTPAF  Q+PG+NGLGLS ARLDL P GVIP+HTHPGASE+L V  G I AGF
Sbjct: 61  TNIINAAVTPAFAAQFPGLNGLGLSTARLDLAPKGVIPMHTHPGASEVLFVLTGSITAGF 120

Query: 121 ISSANAVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPVFSSPSPGLQITDFALFAND 180
           +SSANAV+V+TLK G +M+FPQGLLHFQINAG  +A     F+S +PGLQI DFALFAN 
Sbjct: 121 VSSANAVYVQTLKPGQVMVFPQGLLHFQINAGKSSASAVVTFNSANPGLQILDFALFANS 180

Query: 181 LSTDLVSKTTFLDPALVKKFKAILGGSG 207
           L T+LV  TTFLD   VKK K +LGG+G
Sbjct: 181 LPTELVVGTTFLDATTVKKLKGVLGGTG 208

[Close]

BLAST of Csor.00g158120 vs. NCBI nr
Match: XP_022964299.1 (auxin-binding protein ABP19a-like [Cucurbita moschata] >XP_023514157.1 auxin-binding protein ABP19a-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023514158.1 auxin-binding protein ABP19a-like isoform X2 [Cucurbita pepo subsp. pepo] >KAG6593548.1 Auxin-binding protein ABP19a, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025890.1 Auxin-binding protein ABP19a, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 408 bits (1049), Expect = 1.29e-143
Identity = 206/206 (100.00%), Postives = 206/206 (100.00%), Query Frame = 0


Query: 1   MFHSLFVIALLFSLSNAAVQDFCVADLKGPQGPAGYACKSAVTADDFVYSGLSGAGNITS 60
           MFHSLFVIALLFSLSNAAVQDFCVADLKGPQGPAGYACKSAVTADDFVYSGLSGAGNITS
Sbjct: 1   MFHSLFVIALLFSLSNAAVQDFCVADLKGPQGPAGYACKSAVTADDFVYSGLSGAGNITS 60

Query: 61  LIKAAVTPAFVEQYPGVNGLGLSAARLDLLPGGVIPLHTHPGASELLIVTQGIIQAGFIS 120
           LIKAAVTPAFVEQYPGVNGLGLSAARLDLLPGGVIPLHTHPGASELLIVTQGIIQAGFIS
Sbjct: 61  LIKAAVTPAFVEQYPGVNGLGLSAARLDLLPGGVIPLHTHPGASELLIVTQGIIQAGFIS 120

Query: 121 SANAVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPVFSSPSPGLQITDFALFANDLS 180
           SANAVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPVFSSPSPGLQITDFALFANDLS
Sbjct: 121 SANAVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPVFSSPSPGLQITDFALFANDLS 180

Query: 181 TDLVSKTTFLDPALVKKFKAILGGSG 206
           TDLVSKTTFLDPALVKKFKAILGGSG
Sbjct: 181 TDLVSKTTFLDPALVKKFKAILGGSG 206

[Close]

BLAST of Csor.00g158120 vs. NCBI nr
Match: XP_023000032.1 (auxin-binding protein ABP19a-like [Cucurbita maxima])

HSP 1 Score: 394 bits (1012), Expect = 1.90e-137
Identity = 196/206 (95.15%), Postives = 201/206 (97.57%), Query Frame = 0


Query: 1   MFHSLFVIALLFSLSNAAVQDFCVADLKGPQGPAGYACKSAVTADDFVYSGLSGAGNITS 60
           MFHSLFVI LLFSLSNAAVQDFCVADLKGPQGPAGYACKSAVTADDFVYSGL  AGNITS
Sbjct: 35  MFHSLFVIVLLFSLSNAAVQDFCVADLKGPQGPAGYACKSAVTADDFVYSGLRSAGNITS 94

Query: 61  LIKAAVTPAFVEQYPGVNGLGLSAARLDLLPGGVIPLHTHPGASELLIVTQGIIQAGFIS 120
           +IKAAVTPAFVEQYPGVNG+GLSAARLDLLPGGVIPLHTHPGASELL+VTQGIIQAGFIS
Sbjct: 95  IIKAAVTPAFVEQYPGVNGIGLSAARLDLLPGGVIPLHTHPGASELLLVTQGIIQAGFIS 154

Query: 121 SANAVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPVFSSPSPGLQITDFALFANDLS 180
           SANAVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYP+FSS SPGLQITD+ALFANDLS
Sbjct: 155 SANAVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPIFSSSSPGLQITDYALFANDLS 214

Query: 181 TDLVSKTTFLDPALVKKFKAILGGSG 206
           TDLVSKTTFLDP LVKKFKAILGGSG
Sbjct: 215 TDLVSKTTFLDPVLVKKFKAILGGSG 240

[Close]

BLAST of Csor.00g158120 vs. NCBI nr
Match: XP_038897216.1 (auxin-binding protein ABP19a [Benincasa hispida])

HSP 1 Score: 382 bits (981), Expect = 3.22e-133
Identity = 190/208 (91.35%), Postives = 200/208 (96.15%), Query Frame = 0


Query: 1   MFHSLFVIALLFSLSNAAVQDFCVADLKGPQGPAGYACKSA--VTADDFVYSGLSGAGNI 60
           MFHSL +I+LLFSL NAAVQDFCVADLKGPQGPAGY+CKSA  VTADDFVYSGLSGAGNI
Sbjct: 1   MFHSLLIISLLFSLCNAAVQDFCVADLKGPQGPAGYSCKSAGVVTADDFVYSGLSGAGNI 60

Query: 61  TSLIKAAVTPAFVEQYPGVNGLGLSAARLDLLPGGVIPLHTHPGASELLIVTQGIIQAGF 120
           +SLIKAAVTPAFVEQYPGVNGLGLSAARLDL PGGVIPLHTHPGASELL+VTQGIIQAGF
Sbjct: 61  SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGIIQAGF 120

Query: 121 ISSANAVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPVFSSPSPGLQITDFALFAND 180
           ISSANAV+VKTLKKGDLMIFPQGLLHFQIN GGVTAVG+P+FSSPSPGLQI DFALFAND
Sbjct: 121 ISSANAVYVKTLKKGDLMIFPQGLLHFQINGGGVTAVGFPIFSSPSPGLQILDFALFAND 180

Query: 181 LSTDLVSKTTFLDPALVKKFKAILGGSG 206
           LSTDLVSKTTFLDPAL+KK KA+LGGSG
Sbjct: 181 LSTDLVSKTTFLDPALIKKLKAVLGGSG 208

[Close]

BLAST of Csor.00g158120 vs. NCBI nr
Match: KAA0059402.1 (auxin-binding protein ABP19a [Cucumis melo var. makuwa] >TYK03925.1 auxin-binding protein ABP19a [Cucumis melo var. makuwa])

HSP 1 Score: 376 bits (965), Expect = 8.84e-131
Identity = 186/208 (89.42%), Postives = 198/208 (95.19%), Query Frame = 0


Query: 1   MFHSLFVIALLFSLSNAAVQDFCVADLKGPQGPAGYACK--SAVTADDFVYSGLSGAGNI 60
           MFHSL +I+LLFSLSNAAVQDFCVADLKGPQGPAGY+CK  S VTADDFVY+GL  AGNI
Sbjct: 1   MFHSLLIISLLFSLSNAAVQDFCVADLKGPQGPAGYSCKIASVVTADDFVYTGLRAAGNI 60

Query: 61  TSLIKAAVTPAFVEQYPGVNGLGLSAARLDLLPGGVIPLHTHPGASELLIVTQGIIQAGF 120
           +SLIKAAVTPAFVEQYPGVNGLGLSAARLDL PGGVIPLHTHPGASELL+VTQGII+AGF
Sbjct: 61  SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGIIEAGF 120

Query: 121 ISSANAVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPVFSSPSPGLQITDFALFAND 180
           ISSANAV+VKTLKKGDLM+FPQGLLHFQINAGGVTAVG+PVFSSPSPGLQI DFALFAND
Sbjct: 121 ISSANAVYVKTLKKGDLMVFPQGLLHFQINAGGVTAVGFPVFSSPSPGLQILDFALFAND 180

Query: 181 LSTDLVSKTTFLDPALVKKFKAILGGSG 206
           LSTDLVSKTTFLDP L+KK KA+LGGSG
Sbjct: 181 LSTDLVSKTTFLDPVLIKKLKAVLGGSG 208

[Close]

BLAST of Csor.00g158120 vs. NCBI nr
Match: XP_004141699.1 (auxin-binding protein ABP19a [Cucumis sativus] >KGN45492.1 hypothetical protein Csa_016297 [Cucumis sativus])

HSP 1 Score: 374 bits (959), Expect = 7.26e-130
Identity = 185/208 (88.94%), Postives = 198/208 (95.19%), Query Frame = 0


Query: 1   MFHSLFVIALLFSLSNAAVQDFCVADLKGPQGPAGYACK--SAVTADDFVYSGLSGAGNI 60
           M HSL +I+LLFSLSNAAVQDFCVADLKGPQGPAGY+CK  S VTADDFV+SGL  AGNI
Sbjct: 1   MLHSLLIISLLFSLSNAAVQDFCVADLKGPQGPAGYSCKIASVVTADDFVFSGLRAAGNI 60

Query: 61  TSLIKAAVTPAFVEQYPGVNGLGLSAARLDLLPGGVIPLHTHPGASELLIVTQGIIQAGF 120
           +SLIKAAVTPAFVEQYPGVNGLGLSAARLDL PGGVIPLHTHPGASELL+VTQGII+AGF
Sbjct: 61  SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGIIEAGF 120

Query: 121 ISSANAVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPVFSSPSPGLQITDFALFAND 180
           ISSANAV+VKTLKKGDLM+FPQGLLHFQINAGGVTAVG+PVFSSPSPGLQI DFALFAND
Sbjct: 121 ISSANAVYVKTLKKGDLMVFPQGLLHFQINAGGVTAVGFPVFSSPSPGLQILDFALFAND 180

Query: 181 LSTDLVSKTTFLDPALVKKFKAILGGSG 206
           LSTDLVSKTTFLDPAL+KK K++LGGSG
Sbjct: 181 LSTDLVSKTTFLDPALIKKLKSVLGGSG 208

[Close]

BLAST of Csor.00g158120 vs. ExPASy TrEMBL
Match: A0A6J1HMR8 (Germin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111464356 PE=3 SV=1)

HSP 1 Score: 408 bits (1049), Expect = 6.22e-144
Identity = 206/206 (100.00%), Postives = 206/206 (100.00%), Query Frame = 0


Query: 1   MFHSLFVIALLFSLSNAAVQDFCVADLKGPQGPAGYACKSAVTADDFVYSGLSGAGNITS 60
           MFHSLFVIALLFSLSNAAVQDFCVADLKGPQGPAGYACKSAVTADDFVYSGLSGAGNITS
Sbjct: 1   MFHSLFVIALLFSLSNAAVQDFCVADLKGPQGPAGYACKSAVTADDFVYSGLSGAGNITS 60

Query: 61  LIKAAVTPAFVEQYPGVNGLGLSAARLDLLPGGVIPLHTHPGASELLIVTQGIIQAGFIS 120
           LIKAAVTPAFVEQYPGVNGLGLSAARLDLLPGGVIPLHTHPGASELLIVTQGIIQAGFIS
Sbjct: 61  LIKAAVTPAFVEQYPGVNGLGLSAARLDLLPGGVIPLHTHPGASELLIVTQGIIQAGFIS 120

Query: 121 SANAVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPVFSSPSPGLQITDFALFANDLS 180
           SANAVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPVFSSPSPGLQITDFALFANDLS
Sbjct: 121 SANAVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPVFSSPSPGLQITDFALFANDLS 180

Query: 181 TDLVSKTTFLDPALVKKFKAILGGSG 206
           TDLVSKTTFLDPALVKKFKAILGGSG
Sbjct: 181 TDLVSKTTFLDPALVKKFKAILGGSG 206

[Close]

BLAST of Csor.00g158120 vs. ExPASy TrEMBL
Match: A0A6J1KCE7 (Germin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111494341 PE=3 SV=1)

HSP 1 Score: 394 bits (1012), Expect = 9.19e-138
Identity = 196/206 (95.15%), Postives = 201/206 (97.57%), Query Frame = 0


Query: 1   MFHSLFVIALLFSLSNAAVQDFCVADLKGPQGPAGYACKSAVTADDFVYSGLSGAGNITS 60
           MFHSLFVI LLFSLSNAAVQDFCVADLKGPQGPAGYACKSAVTADDFVYSGL  AGNITS
Sbjct: 35  MFHSLFVIVLLFSLSNAAVQDFCVADLKGPQGPAGYACKSAVTADDFVYSGLRSAGNITS 94

Query: 61  LIKAAVTPAFVEQYPGVNGLGLSAARLDLLPGGVIPLHTHPGASELLIVTQGIIQAGFIS 120
           +IKAAVTPAFVEQYPGVNG+GLSAARLDLLPGGVIPLHTHPGASELL+VTQGIIQAGFIS
Sbjct: 95  IIKAAVTPAFVEQYPGVNGIGLSAARLDLLPGGVIPLHTHPGASELLLVTQGIIQAGFIS 154

Query: 121 SANAVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPVFSSPSPGLQITDFALFANDLS 180
           SANAVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYP+FSS SPGLQITD+ALFANDLS
Sbjct: 155 SANAVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPIFSSSSPGLQITDYALFANDLS 214

Query: 181 TDLVSKTTFLDPALVKKFKAILGGSG 206
           TDLVSKTTFLDP LVKKFKAILGGSG
Sbjct: 215 TDLVSKTTFLDPVLVKKFKAILGGSG 240

[Close]

BLAST of Csor.00g158120 vs. ExPASy TrEMBL
Match: A0A5D3BXR0 (Germin-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001220 PE=3 SV=1)

HSP 1 Score: 376 bits (965), Expect = 4.28e-131
Identity = 186/208 (89.42%), Postives = 198/208 (95.19%), Query Frame = 0


Query: 1   MFHSLFVIALLFSLSNAAVQDFCVADLKGPQGPAGYACK--SAVTADDFVYSGLSGAGNI 60
           MFHSL +I+LLFSLSNAAVQDFCVADLKGPQGPAGY+CK  S VTADDFVY+GL  AGNI
Sbjct: 1   MFHSLLIISLLFSLSNAAVQDFCVADLKGPQGPAGYSCKIASVVTADDFVYTGLRAAGNI 60

Query: 61  TSLIKAAVTPAFVEQYPGVNGLGLSAARLDLLPGGVIPLHTHPGASELLIVTQGIIQAGF 120
           +SLIKAAVTPAFVEQYPGVNGLGLSAARLDL PGGVIPLHTHPGASELL+VTQGII+AGF
Sbjct: 61  SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGIIEAGF 120

Query: 121 ISSANAVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPVFSSPSPGLQITDFALFAND 180
           ISSANAV+VKTLKKGDLM+FPQGLLHFQINAGGVTAVG+PVFSSPSPGLQI DFALFAND
Sbjct: 121 ISSANAVYVKTLKKGDLMVFPQGLLHFQINAGGVTAVGFPVFSSPSPGLQILDFALFAND 180

Query: 181 LSTDLVSKTTFLDPALVKKFKAILGGSG 206
           LSTDLVSKTTFLDP L+KK KA+LGGSG
Sbjct: 181 LSTDLVSKTTFLDPVLIKKLKAVLGGSG 208

[Close]

BLAST of Csor.00g158120 vs. ExPASy TrEMBL
Match: A0A0A0KAW6 (Germin-like protein OS=Cucumis sativus OX=3659 GN=Csa_7G450510 PE=3 SV=1)

HSP 1 Score: 374 bits (959), Expect = 3.52e-130
Identity = 185/208 (88.94%), Postives = 198/208 (95.19%), Query Frame = 0


Query: 1   MFHSLFVIALLFSLSNAAVQDFCVADLKGPQGPAGYACK--SAVTADDFVYSGLSGAGNI 60
           M HSL +I+LLFSLSNAAVQDFCVADLKGPQGPAGY+CK  S VTADDFV+SGL  AGNI
Sbjct: 1   MLHSLLIISLLFSLSNAAVQDFCVADLKGPQGPAGYSCKIASVVTADDFVFSGLRAAGNI 60

Query: 61  TSLIKAAVTPAFVEQYPGVNGLGLSAARLDLLPGGVIPLHTHPGASELLIVTQGIIQAGF 120
           +SLIKAAVTPAFVEQYPGVNGLGLSAARLDL PGGVIPLHTHPGASELL+VTQGII+AGF
Sbjct: 61  SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGIIEAGF 120

Query: 121 ISSANAVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPVFSSPSPGLQITDFALFAND 180
           ISSANAV+VKTLKKGDLM+FPQGLLHFQINAGGVTAVG+PVFSSPSPGLQI DFALFAND
Sbjct: 121 ISSANAVYVKTLKKGDLMVFPQGLLHFQINAGGVTAVGFPVFSSPSPGLQILDFALFAND 180

Query: 181 LSTDLVSKTTFLDPALVKKFKAILGGSG 206
           LSTDLVSKTTFLDPAL+KK K++LGGSG
Sbjct: 181 LSTDLVSKTTFLDPALIKKLKSVLGGSG 208

[Close]

BLAST of Csor.00g158120 vs. ExPASy TrEMBL
Match: A0A6J1D2R7 (Germin-like protein OS=Momordica charantia OX=3673 GN=LOC111017042 PE=3 SV=1)

HSP 1 Score: 365 bits (938), Expect = 5.59e-127
Identity = 181/208 (87.02%), Postives = 194/208 (93.27%), Query Frame = 0


Query: 1   MFHSLFVIALLFSLSNAAVQDFCVADLKGPQGPAGYACKSA--VTADDFVYSGLSGAGNI 60
           M H+LF+I+LLFSLSNAAVQDFCV DLKGPQGPAGY+CKSA  V  DDFV+SGL  AGNI
Sbjct: 1   MLHALFIISLLFSLSNAAVQDFCVGDLKGPQGPAGYSCKSADKVIVDDFVFSGLRAAGNI 60

Query: 61  TSLIKAAVTPAFVEQYPGVNGLGLSAARLDLLPGGVIPLHTHPGASELLIVTQGIIQAGF 120
           +SLIKAAVTPAFVEQYPGVNGLGLSAARLDL PGGV+PLHTHPGASELLIVTQGI++AGF
Sbjct: 61  SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVVPLHTHPGASELLIVTQGIVEAGF 120

Query: 121 ISSANAVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPVFSSPSPGLQITDFALFAND 180
           ISSANAV+VKTLKKGDLM+FPQGLLHFQINAGGVTAV +PVFSSPSPGLQITDFALFAND
Sbjct: 121 ISSANAVYVKTLKKGDLMVFPQGLLHFQINAGGVTAVAFPVFSSPSPGLQITDFALFAND 180

Query: 181 LSTDLVSKTTFLDPALVKKFKAILGGSG 206
           LSTDLV KTTFLDP  VKK KA+LGGSG
Sbjct: 181 LSTDLVVKTTFLDPDQVKKLKAVLGGSG 208

[Close]

BLAST of Csor.00g158120 vs. TAIR 10
Match: AT1G72610.1 (germin-like protein 1 )

HSP 1 Score: 260.4 bits (664), Expect = 1.2e-69
Identity = 133/208 (63.94%), Postives = 160/208 (76.92%), Query Frame = 0


Query: 1   MFHSLFVIALLFSLSNAAVQDFCVADLKGPQGPAGYAC--KSAVTADDFVYSGLSGAGNI 60
           M  ++F+++LLF+LSNA+VQDFCVA+LK  + PAGY C     V A DFV+SGL   GN 
Sbjct: 1   MLRTIFLLSLLFALSNASVQDFCVANLKRAETPAGYPCIRPIHVKATDFVFSGLGTPGNT 60

Query: 61  TSLIKAAVTPAFVEQYPGVNGLGLSAARLDLLPGGVIPLHTHPGASELLIVTQGIIQAGF 120
           T++I AAVTPAF  Q+PG+NGLGLS ARLDL P GVIP+HTHPGASE+L V  G I AGF
Sbjct: 61  TNIINAAVTPAFAAQFPGLNGLGLSTARLDLAPKGVIPMHTHPGASEVLFVLTGSITAGF 120

Query: 121 ISSANAVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPVFSSPSPGLQITDFALFAND 180
           +SSANAV+V+TLK G +M+FPQGLLHFQINAG  +A     F+S +PGLQI DFALFAN 
Sbjct: 121 VSSANAVYVQTLKPGQVMVFPQGLLHFQINAGKSSASAVVTFNSANPGLQILDFALFANS 180

Query: 181 LSTDLVSKTTFLDPALVKKFKAILGGSG 207
           L T+LV  TTFLD   VKK K +LGG+G
Sbjct: 181 LPTELVVGTTFLDATTVKKLKGVLGGTG 208

[Close]

BLAST of Csor.00g158120 vs. TAIR 10
Match: AT5G20630.1 (germin 3 )

HSP 1 Score: 256.9 bits (655), Expect = 1.4e-68
Identity = 128/202 (63.37%), Postives = 159/202 (78.71%), Query Frame = 0


Query: 6   FVIALLFSLSNAAVQDFCVADLKGPQGPAGYACKS--AVTADDFVYSGLSGAGNITSLIK 65
           F+I+L+ ++S A+VQDFCVAD KGPQ P+GY+CK+   VT +DF ++GL  AGN +++IK
Sbjct: 9   FIISLISTISFASVQDFCVADPKGPQSPSGYSCKNPDQVTENDFAFTGLGTAGNTSNIIK 68

Query: 66  AAVTPAFVEQYPGVNGLGLSAARLDLLPGGVIPLHTHPGASELLIVTQGIIQAGFISSAN 125
           AAVTPAF   Y G+NGLG+S ARLDL  GGVIPLHTHPGASE+L+V QG I AGFISSAN
Sbjct: 69  AAVTPAFAPAYAGINGLGVSLARLDLAGGGVIPLHTHPGASEVLVVIQGTICAGFISSAN 128

Query: 126 AVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPVFSSPSPGLQITDFALFANDLSTDL 185
            V++KTL +GD M+FPQGLLHFQ+N+G   A+ +  F S SPGLQI  FALFANDL ++L
Sbjct: 129 KVYLKTLNRGDSMVFPQGLLHFQLNSGKGPALAFVAFGSSSPGLQILPFALFANDLPSEL 188

Query: 186 VSKTTFLDPALVKKFKAILGGS 206
           V  TTFL  A VKK K +LGG+
Sbjct: 189 VEATTFLSDAEVKKLKGVLGGT 210

[Close]

BLAST of Csor.00g158120 vs. TAIR 10
Match: AT1G18980.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 139.4 bits (350), Expect = 3.2e-33
Identity = 80/198 (40.40%), Postives = 117/198 (59.09%), Query Frame = 0


Query: 5   LFVIALLFSLSNAAVQDFCVADLKGPQGPAGYACKSAVTADDFVYSGLSGAGNITSLIKA 64
           +FVI  L S S+  +QDFCV DLK      G+ CKS+V+A DF +SGL G  N ++    
Sbjct: 20  VFVIPSLSSDSD-PLQDFCVGDLKASPSINGFPCKSSVSASDFFFSGLGGPLNTSTPNGV 79

Query: 65  AVTPAFVEQYPGVNGLGLSAARLDLLPGGVIPLHTHPGASELLIVTQGIIQAGFISSANA 124
           AV+PA V  +PG+N LGLS   ++  PGGV P H+HP A+E  +V +G +  GF+++ N 
Sbjct: 80  AVSPANVLTFPGLNTLGLSMNNVEFAPGGVNPPHSHPRATEAGVVIEGSVFVGFLTTNNT 139

Query: 125 VFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPVFSSPSPGLQITDFALFAND--LSTD 184
           +F K L  G++ + P+GL+HFQ N G V A     F+S  PG  +    LF ++  +   
Sbjct: 140 LFSKVLNAGEMFVVPRGLVHFQWNVGKVKARLITSFNSQLPGSAVLPSTLFGSNPTIPNA 199

Query: 185 LVSKTTFLDPALVKKFKA 201
           +++KT   D   V K K+
Sbjct: 200 VLTKTFRTDDVTVNKLKS 216

[Close]

BLAST of Csor.00g158120 vs. TAIR 10
Match: AT1G02335.1 (germin-like protein subfamily 2 member 2 precursor )

HSP 1 Score: 137.5 bits (345), Expect = 1.2e-32
Identity = 79/200 (39.50%), Postives = 117/200 (58.50%), Query Frame = 0


Query: 4   SLFVIALLFSLSNAAVQDFCVADLKGPQGPAGYACKSA--VTADDFVYSGLSGAGNITSL 63
           S  +I  + +    A+QD CVAD        G+ CK    +T  DF ++G+S    I S 
Sbjct: 13  SCIMITSIRAYDPDALQDLCVADKSHGTKLNGFPCKETLNITESDFFFAGISKPAVINST 72

Query: 64  IKAAVTPAFVEQYPGVNGLGLSAARLDLLPGGVIPLHTHPGASELLIVTQGIIQAGFISS 123
           + +AVT A VE+ PG+N L +S AR+D  PGG+ P HTHP A+E++ V +G ++ GFI++
Sbjct: 73  MGSAVTGANVEKIPGLNTLSVSLARIDYAPGGLNPPHTHPRATEVVYVLEGELEVGFITT 132

Query: 124 ANAVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPVFSSPSPGLQITDFALFAND--L 183
           AN +F KT+K G++ +FP+GL+HFQ N G   A     F+S  PG       LFA +  L
Sbjct: 133 ANKLFTKTIKIGEVFVFPRGLVHFQKNNGKSPASVLSAFNSQLPGTASVAATLFAAEPAL 192

Query: 184 STDLVSKTTFLDPALVKKFK 200
             D+++KT  +   +V K K
Sbjct: 193 PEDVLTKTFQVGSKMVDKIK 212

[Close]

BLAST of Csor.00g158120 vs. TAIR 10
Match: AT1G18970.1 (germin-like protein 4 )

HSP 1 Score: 136.0 bits (341), Expect = 3.5e-32
Identity = 79/198 (39.90%), Postives = 113/198 (57.07%), Query Frame = 0


Query: 5   LFVIALLFSLSNAAVQDFCVADLKGPQGPAGYACKSAVTADDFVYSGLSGAGNITSLIKA 64
           LFVI  L S S+  +QDFCV DLK      G+ CKSAV+A DF YSGL G  + ++    
Sbjct: 20  LFVIPSLSSDSD-PLQDFCVGDLKASASINGFPCKSAVSASDFFYSGLGGPLDTSNPNGV 79

Query: 65  AVTPAFVEQYPGVNGLGLSAARLDLLPGGVIPLHTHPGASELLIVTQGIIQAGFISSANA 124
            V PA V  +PG+N LG+S   ++L PGGV P H HP A+E+  V +G +  GF+S+ N 
Sbjct: 80  TVAPANVLTFPGLNTLGISMNNVELAPGGVNPPHLHPRATEVGTVIEGSVFVGFLSTNNT 139

Query: 125 VFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPVFSSPSPGLQITDFALFAN--DLSTD 184
           +F K L  G+  + P+GL+HFQ N G V A     F+S  PG  +    LF +  ++   
Sbjct: 140 LFSKVLNAGEAFVIPRGLVHFQWNVGQVKARMITAFNSQLPGAVVLPSTLFGSKPEIPNA 199

Query: 185 LVSKTTFLDPALVKKFKA 201
           ++++    D   V+  K+
Sbjct: 200 VLTRAFRTDDTTVQNLKS 216

The following BLAST results are available for this feature:

BLAST of Csor.00g158120 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZRA4	4.3e-75	66.67	Auxin-binding protein ABP19a OS=Prunus persica OX=3760 GN=ABP19A PE=3 SV=1	[more]
O04012	8.9e-73	63.77	Auxin-binding protein ABP19b OS=Prunus persica OX=3760 GN=ABP19B PE=3 SV=1	[more]
L8BRS3	1.9e-70	64.04	Germin-like protein OS=Morus alba OX=3498 PE=1 SV=1	[more]
O04011	3.2e-70	62.80	Auxin-binding protein ABP20 OS=Prunus persica OX=3760 GN=ABP20 PE=2 SV=1	[more]
P94040	1.7e-68	63.94	Germin-like protein subfamily 3 member 1 OS=Arabidopsis thaliana OX=3702 GN=GLP1...	[more]

BLAST of Csor.00g158120 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022964299.1	1.29e-143	100.00	auxin-binding protein ABP19a-like [Cucurbita moschata] >XP_023514157.1 auxin-bin...	[more]
XP_023000032.1	1.90e-137	95.15	auxin-binding protein ABP19a-like [Cucurbita maxima]	[more]
XP_038897216.1	3.22e-133	91.35	auxin-binding protein ABP19a [Benincasa hispida]	[more]
KAA0059402.1	8.84e-131	89.42	auxin-binding protein ABP19a [Cucumis melo var. makuwa] >TYK03925.1 auxin-bindin...	[more]
XP_004141699.1	7.26e-130	88.94	auxin-binding protein ABP19a [Cucumis sativus] >KGN45492.1 hypothetical protein ...	[more]

BLAST of Csor.00g158120 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HMR8	6.22e-144	100.00	Germin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111464356 PE=3 SV=1	[more]
A0A6J1KCE7	9.19e-138	95.15	Germin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111494341 PE=3 SV=1	[more]
A0A5D3BXR0	4.28e-131	89.42	Germin-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G...	[more]
A0A0A0KAW6	3.52e-130	88.94	Germin-like protein OS=Cucumis sativus OX=3659 GN=Csa_7G450510 PE=3 SV=1	[more]
A0A6J1D2R7	5.59e-127	87.02	Germin-like protein OS=Momordica charantia OX=3673 GN=LOC111017042 PE=3 SV=1	[more]

BLAST of Csor.00g158120 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G72610.1	1.2e-69	63.94	germin-like protein 1	[more]
AT5G20630.1	1.4e-68	63.37	germin 3	[more]
AT1G18980.1	3.2e-33	40.40	RmlC-like cupins superfamily protein	[more]
AT1G02335.1	1.2e-32	39.50	germin-like protein subfamily 2 member 2 precursor	[more]
AT1G18970.1	3.5e-32	39.90	germin-like protein 4	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001929	Germin	PRINTS	PR00325	GERMIN	coord: 161..176 score: 45.2 coord: 98..118 score: 54.26 coord: 128..148 score: 56.7
IPR006045	Cupin 1	SMART	SM00835	Cupin_1_3	coord: 50..196 e-value: 4.3E-38 score: 142.5
IPR006045	Cupin 1	PFAM	PF00190	Cupin_1	coord: 55..193 e-value: 1.2E-29 score: 102.9
IPR014710	RmlC-like jelly roll fold	GENE3D	2.60.120.10	Jelly Rolls	coord: 16..205 e-value: 2.9E-58 score: 198.6
None	No IPR available	PANTHER	PTHR31238	GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3	coord: 6..202
None	No IPR available	PANTHER	PTHR31238:SF161	GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 1	coord: 6..202
None	No IPR available	CDD	cd02241	cupin_OxOx	coord: 15..202 e-value: 2.495E-76 score: 225.938
IPR019780	Germin, manganese binding site	PROSITE	PS00725	GERMIN	coord: 93..106
IPR011051	RmlC-like cupin domain superfamily	SUPERFAMILY	51182	RmlC-like cupins	coord: 19..199

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g158120.m01	Csor.00g158120.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0048046	apoplast
molecular_function	GO:0030145	manganese ion binding