Csor.00g156470 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g156470
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	trypsin inhibitor 1-like
Location	Csor_Chr19: 7006226 .. 7006522 (-)
RNA-Seq Expression	Csor.00g156470
Synteny	Csor.00g156470

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintronterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTATGTACGTAAGTATATCTTTTGGTAATATTTTCTTATGTTCTTCGTTTGTTAATATACTTTTAGTCTTTACCTAATGCATTCTTTTTTTTATGATCCAGTAAAATCATGGCCAGAACTAAATCTCATGCAAGCTAGCACCGTAGAAAATATTATAAAGAGGCAACATCCCGAGTTTCAGATAGTCATATTGTTAGCAGGAAGTCCAGTAACCAAAGATCTTAAACATGGACGAGTTCGACTGTTTGTTAATATACTCGGTATAGTGGTTGAAATTCCTCGTGAGGGGTAA

mRNA sequence

ATGGCGTATGTACTAAAATCATGGCCAGAACTAAATCTCATGCAAGCTAGCACCGTAGAAAATATTATAAAGAGGCAACATCCCGAGTTTCAGATAGTCATATTGTTAGCAGGAAGTCCAGTAACCAAAGATCTTAAACATGGACGAGTTCGACTGTTTGTTAATATACTCGGTATAGTGGTTGAAATTCCTCGTGAGGGGTAA

Coding sequence (CDS)

ATGGCGTATGTACTAAAATCATGGCCAGAACTAAATCTCATGCAAGCTAGCACCGTAGAAAATATTATAAAGAGGCAACATCCCGAGTTTCAGATAGTCATATTGTTAGCAGGAAGTCCAGTAACCAAAGATCTTAAACATGGACGAGTTCGACTGTTTGTTAATATACTCGGTATAGTGGTTGAAATTCCTCGTGAGGGGTAA

Protein sequence

MAYVLKSWPELNLMQASTVENIIKRQHPEFQIVILLAGSPVTKDLKHGRVRLFVNILGIVVEIPREG

Homology

[Close]

BLAST of Csor.00g156470 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 1.7e-09
Identity = 34/59 (57.63%), Postives = 42/59 (71.19%), Query Frame = 0


Query: 8   WPELNLMQASTVENIIKRQHPEF-QIVILLAGSPVTKDLKHGRVRLFVNILGIVVEIPR 66
           WPEL  + A   + II++++P    I ILL GSPVTKD +  RVRLFVNILG VV+IPR
Sbjct: 51  WPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGDVVQIPR 109

[Close]

BLAST of Csor.00g156470 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 1.9e-08
Identity = 30/61 (49.18%), Postives = 40/61 (65.57%), Query Frame = 0


Query: 7  SWPELNLMQASTVENIIKRQHPEFQIVILLAGSPVTKDLKHGRVRLFVNILGIVVEIPRE 66
          SWP L  +  S  + II+RQ+P  + VIL  G+PVTKD +  RVR++VN  G+VV  PR 
Sbjct: 8  SWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLVVSPPRI 67

Query: 67 G 68
          G
Sbjct: 68 G 68

[Close]

BLAST of Csor.00g156470 vs. ExPASy Swiss-Prot
Match: Q02214 (Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 9.5e-08
Identity = 29/63 (46.03%), Postives = 42/63 (66.67%), Query Frame = 0


Query: 6  KSWPELNLMQASTVENIIKRQHPEF-QIVILLAGSPVTKDLKHGRVRLFVNILGIVVEIP 65
          ++WPEL  + A     II++++ +   +  +L GSPVTKD +  RVRLFVN+L  VV+IP
Sbjct: 32 ETWPELIGVPAKFAREIIQKENSKLTNVPSVLNGSPVTKDFRCERVRLFVNVLDFVVQIP 91

Query: 66 REG 68
          R G
Sbjct: 92 RVG 94

[Close]

BLAST of Csor.00g156470 vs. ExPASy Swiss-Prot
Match: P20076 (Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 PE=3 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 1.6e-07
Identity = 29/63 (46.03%), Postives = 42/63 (66.67%), Query Frame = 0


Query: 6   KSWPELNLMQASTVENIIKRQHPEF-QIVILLAGSPVTKDLKHGRVRLFVNILGIVVEIP 65
           +SWPEL    A   + II++++P+   +  LL GS  T+DL+  RVRLFVN+L IVV+ P
Sbjct: 57  ESWPELLGTPAKFAKQIIQKENPKLTNVETLLNGSAFTEDLRCNRVRLFVNLLDIVVQTP 116

Query: 66  REG 68
           + G
Sbjct: 117 KVG 119

[Close]

BLAST of Csor.00g156470 vs. ExPASy Swiss-Prot
Match: Q03199 (Proteinase inhibitor I-B OS=Nicotiana tabacum OX=4097 GN=TIMPA PE=2 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 8.0e-07
Identity = 27/61 (44.26%), Postives = 40/61 (65.57%), Query Frame = 0


Query: 8   WPELNLMQASTVENIIKRQHPEF-QIVILLAGSPVTKDLKHGRVRLFVNILGIVVEIPRE 67
           WPEL    A     II++++P+   +  +L G+PVT+DL+  RVRLFVN+L  VV+ P+ 
Sbjct: 47  WPELLGTPAKFAMQIIQKENPKLTNVQTVLNGTPVTEDLRCNRVRLFVNVLDFVVQTPQV 106

[Close]

BLAST of Csor.00g156470 vs. NCBI nr
Match: KAG6571807.1 (hypothetical protein SDJN03_28535, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 135 bits (341), Expect = 4.14e-40
Identity = 67/67 (100.00%), Postives = 67/67 (100.00%), Query Frame = 0


Query: 1  MAYVLKSWPELNLMQASTVENIIKRQHPEFQIVILLAGSPVTKDLKHGRVRLFVNILGIV 60
          MAYVLKSWPELNLMQASTVENIIKRQHPEFQIVILLAGSPVTKDLKHGRVRLFVNILGIV
Sbjct: 1  MAYVLKSWPELNLMQASTVENIIKRQHPEFQIVILLAGSPVTKDLKHGRVRLFVNILGIV 60

Query: 61 VEIPREG 67
          VEIPREG
Sbjct: 61 VEIPREG 67

[Close]

BLAST of Csor.00g156470 vs. NCBI nr
Match: KGN60477.1 (hypothetical protein Csa_001998 [Cucumis sativus])

HSP 1 Score: 96.7 bits (239), Expect = 1.70e-24
Identity = 46/63 (73.02%), Postives = 53/63 (84.13%), Query Frame = 0


Query: 5  LKSWPELNLMQASTVENIIKRQHPEFQIVILLAGSPVTKDLKHGRVRLFVNILGIVVEIP 64
          LKSWP LN M A TV NIIK+++P+FQI ILLAGSPVTKDLKHGRVRLF N+  IVV +P
Sbjct: 9  LKSWPALNFMDARTVANIIKKENPDFQIFILLAGSPVTKDLKHGRVRLFTNMEEIVVIVP 68

Query: 65 REG 67
          +EG
Sbjct: 69 QEG 71

[Close]

BLAST of Csor.00g156470 vs. NCBI nr
Match: KGN60478.1 (hypothetical protein Csa_001396 [Cucumis sativus])

HSP 1 Score: 71.6 bits (174), Expect = 1.31e-14
Identity = 34/63 (53.97%), Postives = 44/63 (69.84%), Query Frame = 0


Query: 5  LKSWPELNLMQASTVENIIKRQHPEFQIVILLAGSPVTKDLKHGRVRLFVNILGIVVEIP 64
          LKSWP L  + A TV N IK ++P F+IV +  G+PVTKD+  GRVRL V+  G VV +P
Sbjct: 7  LKSWPALKFLDAQTVANAIKTENPNFEIVTVPEGTPVTKDIIPGRVRLVVDANGKVVNVP 66

Query: 65 REG 67
          +EG
Sbjct: 67 QEG 69

[Close]

BLAST of Csor.00g156470 vs. NCBI nr
Match: XP_011654473.1 (inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetical protein Csa_018430 [Cucumis sativus])

HSP 1 Score: 68.2 bits (165), Expect = 3.60e-13
Identity = 32/61 (52.46%), Postives = 45/61 (73.77%), Query Frame = 0


Query: 7  SWPELNLMQASTVENIIKRQHPEFQIVILLAGSPVTKDLKHGRVRLFVNILGIVVEIPRE 66
          SWPEL  +++S   + I+R  P+ + V+LLAGSPVT+DL+  RVR+FVN+   VVE+PR 
Sbjct: 15 SWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVTEDLRPNRVRIFVNMNDRVVEVPRT 74

[Close]

BLAST of Csor.00g156470 vs. NCBI nr
Match: TMW85239.1 (hypothetical protein EJD97_023458 [Solanum chilense])

HSP 1 Score: 64.7 bits (156), Expect = 1.73e-11
Identity = 34/59 (57.63%), Postives = 43/59 (72.88%), Query Frame = 0


Query: 8   WPELNLMQASTVENIIKRQHPEF-QIVILLAGSPVTKDLKHGRVRLFVNILGIVVEIPR 65
           WPEL  + A   + II++++P    I ILL GSPVTKD +  RVRLFVNILG+VV+IPR
Sbjct: 44  WPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGVVVQIPR 102

[Close]

BLAST of Csor.00g156470 vs. ExPASy TrEMBL
Match: A0A0A0LIF6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914570 PE=3 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 8.25e-25
Identity = 46/63 (73.02%), Postives = 53/63 (84.13%), Query Frame = 0


Query: 5  LKSWPELNLMQASTVENIIKRQHPEFQIVILLAGSPVTKDLKHGRVRLFVNILGIVVEIP 64
          LKSWP LN M A TV NIIK+++P+FQI ILLAGSPVTKDLKHGRVRLF N+  IVV +P
Sbjct: 9  LKSWPALNFMDARTVANIIKKENPDFQIFILLAGSPVTKDLKHGRVRLFTNMEEIVVIVP 68

Query: 65 REG 67
          +EG
Sbjct: 69 QEG 71

[Close]

BLAST of Csor.00g156470 vs. ExPASy TrEMBL
Match: A0A0A0LFD4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914580 PE=3 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 6.37e-15
Identity = 34/63 (53.97%), Postives = 44/63 (69.84%), Query Frame = 0


Query: 5  LKSWPELNLMQASTVENIIKRQHPEFQIVILLAGSPVTKDLKHGRVRLFVNILGIVVEIP 64
          LKSWP L  + A TV N IK ++P F+IV +  G+PVTKD+  GRVRL V+  G VV +P
Sbjct: 7  LKSWPALKFLDAQTVANAIKTENPNFEIVTVPEGTPVTKDIIPGRVRLVVDANGKVVNVP 66

Query: 65 REG 67
          +EG
Sbjct: 67 QEG 69

[Close]

BLAST of Csor.00g156470 vs. ExPASy TrEMBL
Match: A0A0A0KPI0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.74e-13
Identity = 32/61 (52.46%), Postives = 45/61 (73.77%), Query Frame = 0


Query: 7  SWPELNLMQASTVENIIKRQHPEFQIVILLAGSPVTKDLKHGRVRLFVNILGIVVEIPRE 66
          SWPEL  +++S   + I+R  P+ + V+LLAGSPVT+DL+  RVR+FVN+   VVE+PR 
Sbjct: 15 SWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVTEDLRPNRVRIFVNMNDRVVEVPRT 74

[Close]

BLAST of Csor.00g156470 vs. ExPASy TrEMBL
Match: A0A6N2AU12 (Uncharacterized protein OS=Solanum chilense OX=4083 GN=EJD97_023458 PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 8.39e-12
Identity = 34/59 (57.63%), Postives = 43/59 (72.88%), Query Frame = 0


Query: 8   WPELNLMQASTVENIIKRQHPEF-QIVILLAGSPVTKDLKHGRVRLFVNILGIVVEIPR 65
           WPEL  + A   + II++++P    I ILL GSPVTKD +  RVRLFVNILG+VV+IPR
Sbjct: 44  WPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGVVVQIPR 102

[Close]

BLAST of Csor.00g156470 vs. ExPASy TrEMBL
Match: A0A1S3YJI6 (trypsin inhibitor 1-like OS=Nicotiana tabacum OX=4097 GN=LOC107776717 PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.32e-11
Identity = 32/63 (50.79%), Postives = 45/63 (71.43%), Query Frame = 0


Query: 6  KSWPELNLMQASTVENIIKRQHPEF-QIVILLAGSPVTKDLKHGRVRLFVNILGIVVEIP 65
          ++WPEL  + A     II++++P+   +  LL GSPVTKDL+  RVRLFVN+L IVV++P
Sbjct: 32 ETWPELIGVPAKFAREIIQKENPKLTNVPSLLNGSPVTKDLRCNRVRLFVNVLDIVVQLP 91

Query: 66 REG 67
          R G
Sbjct: 92 RVG 94

[Close]

BLAST of Csor.00g156470 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 52.4 bits (124), Expect = 1.7e-07
Identity = 25/61 (40.98%), Postives = 36/61 (59.02%), Query Frame = 0


Query: 7  SWPELNLMQASTVENIIKRQHPEFQIVILLAGSPVTKDLKHGRVRLFVNILGIVVEIPRE 66
          SWPEL          +I+R++P     ++L GSPVT D +  RVR+FV+   IVV+ P+ 
Sbjct: 10 SWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNRIVVKTPKS 69

Query: 67 G 68
          G
Sbjct: 70 G 70

[Close]

BLAST of Csor.00g156470 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 46.6 bits (109), Expect = 9.1e-06
Identity = 23/58 (39.66%), Postives = 37/58 (63.79%), Query Frame = 0


Query: 7  SWPELNLMQASTVENIIKRQHPEFQIVILLAGSPVTKDLKHGRVRLFVNILGIVVEIP 65
          SWPEL   +    + +I+R++P+ + VI+L G+ V +     RV ++VN  GIVV+IP
Sbjct: 39 SWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEIFICSRVYVWVNDCGIVVQIP 96

[Close]

BLAST of Csor.00g156470 vs. TAIR 10
Match: AT2G38900.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 43.1 bits (100), Expect = 1.0e-04
Identity = 22/61 (36.07%), Postives = 35/61 (57.38%), Query Frame = 0


Query: 7  SWPELNLMQASTVENIIKRQHPEFQIVILLAGSPVTKDLKHGRVRLFVNILGIVVEIPRE 66
          SWPEL         ++IK ++    +V++  G+ VT+DL   RVR++V+ + IVV  P  
Sbjct: 13 SWPELLGTNGDYAASVIKGENSSLNVVVVSDGNYVTEDLSCYRVRVWVDEIRIVVRNPTA 72

Query: 67 G 68
          G
Sbjct: 73 G 73

[Close]

BLAST of Csor.00g156470 vs. TAIR 10
Match: AT2G38900.2 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 43.1 bits (100), Expect = 1.0e-04
Identity = 22/61 (36.07%), Postives = 35/61 (57.38%), Query Frame = 0


Query: 7  SWPELNLMQASTVENIIKRQHPEFQIVILLAGSPVTKDLKHGRVRLFVNILGIVVEIPRE 66
          SWPEL         ++IK ++    +V++  G+ VT+DL   RVR++V+ + IVV  P  
Sbjct: 28 SWPELLGTNGDYAASVIKGENSSLNVVVVSDGNYVTEDLSCYRVRVWVDEIRIVVRNPTA 87

Query: 67 G 68
          G
Sbjct: 88 G 88

The following BLAST results are available for this feature:

BLAST of Csor.00g156470 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P16231	1.7e-09	57.63	Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1	[more]
P19873	1.9e-08	49.18	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
Q02214	9.5e-08	46.03	Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1	[more]
P20076	1.6e-07	46.03	Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 PE=3 ...	[more]
Q03199	8.0e-07	44.26	Proteinase inhibitor I-B OS=Nicotiana tabacum OX=4097 GN=TIMPA PE=2 SV=1	[more]

BLAST of Csor.00g156470 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6571807.1	4.14e-40	100.00	hypothetical protein SDJN03_28535, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KGN60477.1	1.70e-24	73.02	hypothetical protein Csa_001998 [Cucumis sativus]	[more]
KGN60478.1	1.31e-14	53.97	hypothetical protein Csa_001396 [Cucumis sativus]	[more]
XP_011654473.1	3.60e-13	52.46	inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetic...	[more]
TMW85239.1	1.73e-11	57.63	hypothetical protein EJD97_023458 [Solanum chilense]	[more]

BLAST of Csor.00g156470 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LIF6	8.25e-25	73.02	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914570 PE=3 SV=1	[more]
A0A0A0LFD4	6.37e-15	53.97	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914580 PE=3 SV=1	[more]
A0A0A0KPI0	1.74e-13	52.46	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1	[more]
A0A6N2AU12	8.39e-12	57.63	Uncharacterized protein OS=Solanum chilense OX=4083 GN=EJD97_023458 PE=3 SV=1	[more]
A0A1S3YJI6	1.32e-11	50.79	trypsin inhibitor 1-like OS=Nicotiana tabacum OX=4097 GN=LOC107776717 PE=3 SV=1	[more]

BLAST of Csor.00g156470 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38870.1	1.7e-07	40.98	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT5G43580.1	9.1e-06	39.66	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT2G38900.1	1.0e-04	36.07	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT2G38900.2	1.0e-04	36.07	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRINTS	PR00292	POTATOINHBTR	coord: 44..54 score: 53.36 coord: 32..43 score: 50.0 coord: 7..20 score: 33.54
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 7..66 e-value: 1.7E-15 score: 57.4
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 7..67
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 4..67 e-value: 9.5E-17 score: 63.1
None	No IPR available	PANTHER	PTHR33091:SF83	ETHYLENE-RESPONSIVE PROTEINASE INHIBITOR 1	coord: 7..67
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 5..67

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g156470.m01	Csor.00g156470.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity