Csor.00g154460 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g154460
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptioninhibitor of trypsin and hageman factor-like
LocationCsor_Chr19: 442605 .. 442958 (-)
RNA-Seq ExpressionCsor.00g154460
SyntenyCsor.00g154460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintronterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATGATTTTTGTCCTCCCGGTGGGTTTCTCACCATTTCTCGTTTTAGTACTTCTATCCTCGAGATTTGTTTTGTGATGAAACTATACGATTTTTAGAATAATTTCAATTAAGTTTTGAAAATCATTGATTTTTTTATTTGATTTGGTTTACCAGGAAAGAATAGGTGGCCCGAACTTGTTGGAATAGAAGCAAGGATTGTAGAGAAAATCATCAAGAAAGAAAATCCCAATGTAGGGATTGTTCAAATTTTCTTAGCGGGAAGTCCTGTGCCGTTAGATTTTAGCTGCCATCGAGTTCGATTGTTTGTTAATATAAATGATGTAGTTGTTAGCATACCATGGACTGGTTAG

mRNA sequence

ATGAATGATTTTTGTCCTCCCGGAAAGAATAGGTGGCCCGAACTTGTTGGAATAGAAGCAAGGATTGTAGAGAAAATCATCAAGAAAGAAAATCCCAATGTAGGGATTGTTCAAATTTTCTTAGCGGGAAGTCCTGTGCCGTTAGATTTTAGCTGCCATCGAGTTCGATTGTTTGTTAATATAAATGATGTAGTTGTTAGCATACCATGGACTGGTTAG

Coding sequence (CDS)

ATGAATGATTTTTGTCCTCCCGGAAAGAATAGGTGGCCCGAACTTGTTGGAATAGAAGCAAGGATTGTAGAGAAAATCATCAAGAAAGAAAATCCCAATGTAGGGATTGTTCAAATTTTCTTAGCGGGAAGTCCTGTGCCGTTAGATTTTAGCTGCCATCGAGTTCGATTGTTTGTTAATATAAATGATGTAGTTGTTAGCATACCATGGACTGGTTAG

Protein sequence

MNDFCPPGKNRWPELVGIEARIVEKIIKKENPNVGIVQIFLAGSPVPLDFSCHRVRLFVNINDVVVSIPWTG
Homology
BLAST of Csor.00g154460 vs. ExPASy Swiss-Prot
Match: P05118 (Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=2 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 3.6e-13
Identity = 31/62 (50.00%), Postives = 45/62 (72.58%), Query Frame = 0

Query: 8   GKNRWPELVGIEARIVEKIIKKENPNVGIVQIFLAGSPVPLDFSCHRVRLFVNINDVVVS 67
           GK  WPEL+G+  ++ ++II+KENP++  + I L+GSP+ LD+ C RVRLF NI   VV 
Sbjct: 47  GKQMWPELIGVPTKLAKEIIEKENPSITNIPILLSGSPITLDYLCDRVRLFDNILGFVVQ 106

Query: 68  IP 70
           +P
Sbjct: 107 MP 108

BLAST of Csor.00g154460 vs. ExPASy Swiss-Prot
Match: Q00783 (Proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 3.6e-13
Identity = 36/72 (50.00%), Postives = 45/72 (62.50%), Query Frame = 0

Query: 1   MNDFCPPGKNRWPELVGIEARIVEKIIKKENPNVGIVQIFLAGSPVPLDFSCHRVRLFVN 60
           + +F   GK RWPEL+G+  ++ + II+KEN  +  V I L GSPV LD  C RVRLF N
Sbjct: 36  LKEFQCKGKLRWPELIGVPTKLAKGIIEKENSLISNVHILLNGSPVTLDIRCDRVRLFDN 95

Query: 61  INDVVVSIPWTG 73
           I   VV IP  G
Sbjct: 96  ILGYVVDIPVVG 107

BLAST of Csor.00g154460 vs. ExPASy Swiss-Prot
Match: P08454 (Wound-induced proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=1 SV=2)

HSP 1 Score: 74.3 bits (181), Expect = 6.2e-13
Identity = 34/62 (54.84%), Postives = 44/62 (70.97%), Query Frame = 0

Query: 8   GKNRWPELVGIEARIVEKIIKKENPNVGIVQIFLAGSPVPLDFSCHRVRLFVNINDVVVS 67
           GK RWPEL+G+  ++ + II+KEN  +  VQI L GSPV +D+ C+RVRLF NI   VV 
Sbjct: 43  GKQRWPELIGVPTKLAKGIIEKENSLITNVQILLNGSPVTMDYRCNRVRLFDNILGDVVQ 102

Query: 68  IP 70
           IP
Sbjct: 103 IP 104

BLAST of Csor.00g154460 vs. ExPASy Swiss-Prot
Match: Q02214 (Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 6.2e-13
Identity = 36/69 (52.17%), Postives = 43/69 (62.32%), Query Frame = 0

Query: 5  CPPGKNR-WPELVGIEARIVEKIIKKENPNVGIVQIFLAGSPVPLDFSCHRVRLFVNIND 64
          CP  K   WPEL+G+ A+   +II+KEN  +  V   L GSPV  DF C RVRLFVN+ D
Sbjct: 26 CPGNKKETWPELIGVPAKFAREIIQKENSKLTNVPSVLNGSPVTKDFRCERVRLFVNVLD 85

Query: 65 VVVSIPWTG 73
           VV IP  G
Sbjct: 86 FVVQIPRVG 94

BLAST of Csor.00g154460 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 1.1e-12
Identity = 35/62 (56.45%), Postives = 42/62 (67.74%), Query Frame = 0

Query: 8   GKNRWPELVGIEARIVEKIIKKENPNVGIVQIFLAGSPVPLDFSCHRVRLFVNINDVVVS 67
           GK  WPEL+G+ A   + II+KENP++  + I L GSPV  DF C RVRLFVNI   VV 
Sbjct: 47  GKQFWPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGDVVQ 106

Query: 68  IP 70
           IP
Sbjct: 107 IP 108

BLAST of Csor.00g154460 vs. NCBI nr
Match: KAG6571499.1 (Proteinase inhibitor I-B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 160 bits (404), Expect = 1.42e-49
Identity = 72/72 (100.00%), Postives = 72/72 (100.00%), Query Frame = 0

Query: 1  MNDFCPPGKNRWPELVGIEARIVEKIIKKENPNVGIVQIFLAGSPVPLDFSCHRVRLFVN 60
          MNDFCPPGKNRWPELVGIEARIVEKIIKKENPNVGIVQIFLAGSPVPLDFSCHRVRLFVN
Sbjct: 1  MNDFCPPGKNRWPELVGIEARIVEKIIKKENPNVGIVQIFLAGSPVPLDFSCHRVRLFVN 60

Query: 61 INDVVVSIPWTG 72
          INDVVVSIPWTG
Sbjct: 61 INDVVVSIPWTG 72

BLAST of Csor.00g154460 vs. NCBI nr
Match: XP_022963567.1 (inhibitor of trypsin and hageman factor-like [Cucurbita moschata])

HSP 1 Score: 115 bits (287), Expect = 1.08e-31
Identity = 54/74 (72.97%), Postives = 59/74 (79.73%), Query Frame = 0

Query: 1  MNDFCPPGKNRWPELVGIEARIVEKIIKKENPNVGIVQIFLAGSP--VPLDFSCHRVRLF 60
          M DFCPPGKNRWPELVGIEARI EKII+KENPNV IVQIFL GS   + ++F C RVRL 
Sbjct: 1  MADFCPPGKNRWPELVGIEARIAEKIIQKENPNVDIVQIFLFGSKSGITMEFCCSRVRLL 60

Query: 61 VNINDVVVSIPWTG 72
          VNIN V+V IP  G
Sbjct: 61 VNINGVIVGIPSIG 74

BLAST of Csor.00g154460 vs. NCBI nr
Match: KAG6571493.1 (Proteinase inhibitor I-B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 107 bits (268), Expect = 8.34e-29
Identity = 52/74 (70.27%), Postives = 57/74 (77.03%), Query Frame = 0

Query: 1  MNDFCPPGKNRWPELVGIEARIVEKIIKKENPNVGIVQIFLAGS--PVPLDFSCHRVRLF 60
          M DFCP GK RWPELVG EARI EKII+KENPNV IVQIFL GS  P+ ++F C RVRL 
Sbjct: 1  MGDFCP-GKKRWPELVGTEARIAEKIIQKENPNVNIVQIFLFGSKSPISMEFCCSRVRLL 60

Query: 61 VNINDVVVSIPWTG 72
          VNIN V+V IP  G
Sbjct: 61 VNINGVIVGIPSIG 73

BLAST of Csor.00g154460 vs. NCBI nr
Match: KAG6571494.1 (hypothetical protein SDJN03_28222, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 99.8 bits (247), Expect = 1.30e-25
Identity = 44/72 (61.11%), Postives = 52/72 (72.22%), Query Frame = 0

Query: 1  MNDFCPPGKNRWPELVGIEARIVEKIIKKENPNVGIVQIFLAGSPVPLDFSCHRVRLFVN 60
          M DFCPPGKNRWPELVGI++ I E II+K+N NV  V+  LAGS V  D  C RVR+FVN
Sbjct: 1  MADFCPPGKNRWPELVGIKSTIAEHIIRKDNSNVEHVETILAGSAVTEDLRCDRVRIFVN 60

Query: 61 INDVVVSIPWTG 72
          I  ++V IP  G
Sbjct: 61 IKGIIVQIPTIG 72

BLAST of Csor.00g154460 vs. NCBI nr
Match: KGN50840.1 (hypothetical protein Csa_004718 [Cucumis sativus])

HSP 1 Score: 94.0 bits (232), Expect = 2.51e-23
Identity = 41/72 (56.94%), Postives = 51/72 (70.83%), Query Frame = 0

Query: 1  MNDFCPPGKNRWPELVGIEARIVEKIIKKENPNVGIVQIFLAGSPVPLDFSCHRVRLFVN 60
          M D CPPGKN+WPELVG++A   + IIKK+NPNV  V + LAGS    D  C RV +FVN
Sbjct: 1  MADICPPGKNQWPELVGVKATTAKYIIKKDNPNVENVVVLLAGSGTTEDIRCDRVWVFVN 60

Query: 61 INDVVVSIPWTG 72
          I+D+VV +P  G
Sbjct: 61 IHDLVVEVPKVG 72

BLAST of Csor.00g154460 vs. ExPASy TrEMBL
Match: A0A6J1HFK9 (inhibitor of trypsin and hageman factor-like OS=Cucurbita moschata OX=3662 GN=LOC111463862 PE=3 SV=1)

HSP 1 Score: 115 bits (287), Expect = 5.24e-32
Identity = 54/74 (72.97%), Postives = 59/74 (79.73%), Query Frame = 0

Query: 1  MNDFCPPGKNRWPELVGIEARIVEKIIKKENPNVGIVQIFLAGSP--VPLDFSCHRVRLF 60
          M DFCPPGKNRWPELVGIEARI EKII+KENPNV IVQIFL GS   + ++F C RVRL 
Sbjct: 1  MADFCPPGKNRWPELVGIEARIAEKIIQKENPNVDIVQIFLFGSKSGITMEFCCSRVRLL 60

Query: 61 VNINDVVVSIPWTG 72
          VNIN V+V IP  G
Sbjct: 61 VNINGVIVGIPSIG 74

BLAST of Csor.00g154460 vs. ExPASy TrEMBL
Match: A0A0A0KME7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286040 PE=3 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 1.22e-23
Identity = 41/72 (56.94%), Postives = 51/72 (70.83%), Query Frame = 0

Query: 1  MNDFCPPGKNRWPELVGIEARIVEKIIKKENPNVGIVQIFLAGSPVPLDFSCHRVRLFVN 60
          M D CPPGKN+WPELVG++A   + IIKK+NPNV  V + LAGS    D  C RV +FVN
Sbjct: 1  MADICPPGKNQWPELVGVKATTAKYIIKKDNPNVENVVVLLAGSGTTEDIRCDRVWVFVN 60

Query: 61 INDVVVSIPWTG 72
          I+D+VV +P  G
Sbjct: 61 IHDLVVEVPKVG 72

BLAST of Csor.00g154460 vs. ExPASy TrEMBL
Match: A0A0M3SAG8 (Serine proteinase inhibitor OS=Cucumis metulifer OX=61886 PE=2 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 4.07e-22
Identity = 41/72 (56.94%), Postives = 49/72 (68.06%), Query Frame = 0

Query: 1  MNDFCPPGKNRWPELVGIEARIVEKIIKKENPNVGIVQIFLAGSPVPLDFSCHRVRLFVN 60
          M D CPPGKN+WPELVGI+A   E II+K+NP+V  V + LAG+    D  C RV LFVN
Sbjct: 1  MADICPPGKNQWPELVGIKATTAEYIIRKDNPSVEKVIVLLAGTGTTKDIRCDRVWLFVN 60

Query: 61 INDVVVSIPWTG 72
          I + VV IP  G
Sbjct: 61 IEEKVVEIPKVG 72

BLAST of Csor.00g154460 vs. ExPASy TrEMBL
Match: A0A0A0KMM0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G285030 PE=3 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 1.59e-19
Identity = 40/72 (55.56%), Postives = 47/72 (65.28%), Query Frame = 0

Query: 1  MNDFCPPGKNRWPELVGIEARIVEKIIKKENPNVGIVQIFLAGSPVPLDFSCHRVRLFVN 60
          M D C P KN+WPELVGI+A I E IIKK+NPNV  V + LAG+    DF   RV LF N
Sbjct: 1  MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLFTN 60

Query: 61 INDVVVSIPWTG 72
          I+  VV +P  G
Sbjct: 61 IDGRVVEMPTVG 72

BLAST of Csor.00g154460 vs. ExPASy TrEMBL
Match: E9JGS6 (Serine proteinase inhibitor 1a (Fragment) OS=Solanum nigrum OX=4112 GN=SPI1a PE=3 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 5.25e-19
Identity = 37/64 (57.81%), Postives = 46/64 (71.88%), Query Frame = 0

Query: 9  KNRWPELVGIEARIVEKIIKKENPNVGIVQIFLAGSPVPLDFSCHRVRLFVNINDVVVSI 68
          K RWPELVG+ A++ + II+KENP +  V I L GSPV  DFSC+RVR+F+NI   VV I
Sbjct: 1  KQRWPELVGVPAKLAKGIIEKENPFITHVPILLIGSPVTFDFSCNRVRIFINIFGNVVDI 60

Query: 69 PWTG 72
          P  G
Sbjct: 61 PIVG 64

BLAST of Csor.00g154460 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 65.5 bits (158), Expect = 2.0e-11
Identity = 31/66 (46.97%), Postives = 39/66 (59.09%), Query Frame = 0

Query: 7  PGKNRWPELVGIEARIVEKIIKKENPNVGIVQIFLAGSPVPLDFSCHRVRLFVNINDVVV 66
          P KN WPEL G        +I++ENP V    + L GSPV  DF C RVR+FV+ N +VV
Sbjct: 6  PRKNSWPELTGTNGDYAAVVIERENPTVN-AAVILDGSPVTADFRCDRVRVFVDGNRIVV 65

Query: 67 SIPWTG 73
            P +G
Sbjct: 66 KTPKSG 70

BLAST of Csor.00g154460 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 58.5 bits (140), Expect = 2.5e-09
Identity = 29/66 (43.94%), Postives = 42/66 (63.64%), Query Frame = 0

Query: 7  PGKNRWPELVGIEARIVEKIIKKENPNVGIVQIFLAGSPVPLDFSCHRVRLFVNINDVVV 66
          PGK+ WPEL+G +    +++I++ENP +  V I L G+ VP  F C RV ++VN   +VV
Sbjct: 35 PGKSSWPELLGAKGEDAKEVIERENPKMKAV-IILDGTVVPEIFICSRVYVWVNDCGIVV 94

Query: 67 SIPWTG 73
           IP  G
Sbjct: 95 QIPIIG 99

BLAST of Csor.00g154460 vs. TAIR 10
Match: AT2G38900.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 52.0 bits (123), Expect = 2.3e-07
Identity = 26/65 (40.00%), Postives = 38/65 (58.46%), Query Frame = 0

Query: 8  GKNRWPELVGIEARIVEKIIKKENPNVGIVQIFLAGSPVPLDFSCHRVRLFVNINDVVVS 67
          GKN WPEL+G        +IK EN ++ +V +   G+ V  D SC+RVR++V+   +VV 
Sbjct: 10 GKNSWPELLGTNGDYAASVIKGENSSLNVV-VVSDGNYVTEDLSCYRVRVWVDEIRIVVR 69

Query: 68 IPWTG 73
           P  G
Sbjct: 70 NPTAG 73

BLAST of Csor.00g154460 vs. TAIR 10
Match: AT2G38900.2 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 52.0 bits (123), Expect = 2.3e-07
Identity = 26/65 (40.00%), Postives = 38/65 (58.46%), Query Frame = 0

Query: 8  GKNRWPELVGIEARIVEKIIKKENPNVGIVQIFLAGSPVPLDFSCHRVRLFVNINDVVVS 67
          GKN WPEL+G        +IK EN ++ +V +   G+ V  D SC+RVR++V+   +VV 
Sbjct: 25 GKNSWPELLGTNGDYAASVIKGENSSLNVV-VVSDGNYVTEDLSCYRVRVWVDEIRIVVR 84

Query: 68 IPWTG 73
           P  G
Sbjct: 85 NPTAG 88

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P051183.6e-1350.00Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=... [more]
Q007833.6e-1350.00Proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=3 SV=1[more]
P084546.2e-1354.84Wound-induced proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=1 SV=2[more]
Q022146.2e-1352.17Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1[more]
P162311.1e-1256.45Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KAG6571499.11.42e-49100.00Proteinase inhibitor I-B, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022963567.11.08e-3172.97inhibitor of trypsin and hageman factor-like [Cucurbita moschata][more]
KAG6571493.18.34e-2970.27Proteinase inhibitor I-B, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG6571494.11.30e-2561.11hypothetical protein SDJN03_28222, partial [Cucurbita argyrosperma subsp. sorori... [more]
KGN50840.12.51e-2356.94hypothetical protein Csa_004718 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A6J1HFK95.24e-3272.97inhibitor of trypsin and hageman factor-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A0A0KME71.22e-2356.94Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286040 PE=3 SV=1[more]
A0A0M3SAG84.07e-2256.94Serine proteinase inhibitor OS=Cucumis metulifer OX=61886 PE=2 SV=1[more]
A0A0A0KMM01.59e-1955.56Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G285030 PE=3 SV=1[more]
E9JGS65.25e-1957.81Serine proteinase inhibitor 1a (Fragment) OS=Solanum nigrum OX=4112 GN=SPI1a PE=... [more]
Match NameE-valueIdentityDescription
AT2G38870.12.0e-1146.97Serine protease inhibitor, potato inhibitor I-type family protein [more]
AT5G43580.12.5e-0943.94Serine protease inhibitor, potato inhibitor I-type family protein [more]
AT2G38900.12.3e-0740.00Serine protease inhibitor, potato inhibitor I-type family protein [more]
AT2G38900.22.3e-0740.00Serine protease inhibitor, potato inhibitor I-type family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000864Proteinase inhibitor I13, potato inhibitor IPRINTSPR00292POTATOINHBTRcoord: 11..24
score: 41.3
coord: 49..59
score: 60.08
coord: 37..48
score: 42.75
IPR000864Proteinase inhibitor I13, potato inhibitor IPFAMPF00280potato_inhibitcoord: 9..72
e-value: 6.8E-21
score: 74.7
IPR000864Proteinase inhibitor I13, potato inhibitor IPANTHERPTHR33091PROTEIN, PUTATIVE, EXPRESSED-RELATEDcoord: 4..72
NoneNo IPR availableGENE3D3.30.10.10Trypsin Inhibitor V, subunit Acoord: 3..70
e-value: 5.6E-22
score: 79.8
NoneNo IPR availablePANTHERPTHR33091:SF83ETHYLENE-RESPONSIVE PROTEINASE INHIBITOR 1coord: 4..72
IPR036354Proteinase inhibitor I13, potato inhibitor I superfamilySUPERFAMILY54654CI-2 family of serine protease inhibitorscoord: 5..72

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g154460.m01Csor.00g154460.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010951 negative regulation of endopeptidase activity
biological_process GO:0009611 response to wounding
molecular_function GO:0004867 serine-type endopeptidase inhibitor activity