Csor.00g151230 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g151230
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionRegulator of Vps4 activity in the MVB pathway protein, putative
LocationCsor_Chr14: 4214616 .. 4216007 (-)
RNA-Seq ExpressionCsor.00g151230
SyntenyCsor.00g151230
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAAGGAAACTGGATGCTCTACTAGGAAGGAACAGATATTTGAAGCCTTCCAAGATCAAAATCCTTGTAAACATGGCCATTTCCAGGACTGCCATTCTCAAAAACCAGCACCGTGCTCGCTGCTCCCTTGCCCGGGCCGACGTTCTTCAGCTCTTGAACCTCAGTTACCAACACCGCGCTCAGCTTAGAGTAATTTGCTTCTCCCTCTAAACTCTATAGTTCAGCCCCTTCTCCTCTTTTTTCAATTGCTGAGCTTGGATGTGAATGAACAGGTTGAGATTGTGATAAAGGAGAACAACATGATGGATGCACTTGGAATGATAGAGGATTACTGTTCCCTCCTCATAGAAAAAGTGGCTCTACTTCAAATGAACAAGTAGGACATCTCTTTGAATGCAGCCCTTTTAAATGAAACCAAACCAAACCTTTCCTGATTCTCTTTTGGCTTAAATGCAGGGAATGCCCTGGTGAAGTTAAGGAGGCAATATCAAGTCTGATCTTTGCATCTTCAAGGTTTGGAGAGTTTCCCGAACTCCAGGAGATTCGTCGGATTTTCGAGCTGAAATTCGGGAAAGAGTTTGCTAGCAGTGCCGTTAATTTACGCAACAATTGTGGAGTTAGTCCCAAGGTGAGTTTGATATTAGTTGCCTACATGCGTGTTTGATATCAACAGGTTTGTATCTCACAGCTTCCATGTTACAGATGATCCAAAAGTTTTCAACAAAGCAGCCCAACGCTGAAACTAAACTGAAAGTTCTAAAGGAAATTGCTTCTGAGAATGGCATAACTCTGCATTTAGAGGAAGAACCTGCAATAGTCATCAAGGTAAATCAGAAGATGGAAACCAAGAAATCAGGAGACTTAGATAACCCAGAAGTCATGAACACCACAGATAATCCACATGAAGTTATTATGAGTGAGGAGACAGCATCAGAATCAGTGAGAGCAAAGAAGTTTAAAGATGCAGCTAGTGCCGCTCAAGAAGCCTTTCAATCAGCAGCTTATGCAGCATTAGCAGCTAAAGCAGCGATCGAACTTTCAAGATCTGAATCACAGGATATTCATATAGAGGAAAAAAACTCAGATTTGGAGAGTTCTCATGAACCCAAGATTAATCCTGATGAGCTTGAAAGTTGCAGTTCAGAATATGAAGATCAAGACGCAGCAGAAAGCAATCAAGAAGAAATACCAGAAGAACCAGCCAAAGAAATTATGTTGTCTGATCCGAATTTGGATTCAGATATTAGCAATCTGAAGGAGCAATCATCATCATCTAAACCATATTCGATCAGTGAGCCAGAAAGTGACAAGTTAATCAGTAAAGAAGATGAAGTTGTAATTAAAAACAAAATTTCAGAAGAGGATATCAGAGTTGAGGAATCAAAGTAA

mRNA sequence

ATGGGAAGGAAACTGGATGCTCTACTAGGAAGGAACAGATATTTGAAGCCTTCCAAGATCAAAATCCTTGTAAACATGGCCATTTCCAGGACTGCCATTCTCAAAAACCAGCACCGTGCTCGCTGCTCCCTTGCCCGGGCCGACGTTCTTCAGCTCTTGAACCTCAGTTACCAACACCGCGCTCAGCTTAGAGTTGAGATTGTGATAAAGGAGAACAACATGATGGATGCACTTGGAATGATAGAGGATTACTGTTCCCTCCTCATAGAAAAAGTGGCTCTACTTCAAATGAACAAGGAATGCCCTGGTGAAGTTAAGGAGGCAATATCAAGTCTGATCTTTGCATCTTCAAGGTTTGGAGAGTTTCCCGAACTCCAGGAGATTCGTCGGATTTTCGAGCTGAAATTCGGGAAAGAGTTTGCTAGCAGTGCCGTTAATTTACGCAACAATTGTGGAGTTAGTCCCAAGATGATCCAAAAGTTTTCAACAAAGCAGCCCAACGCTGAAACTAAACTGAAAGTTCTAAAGGAAATTGCTTCTGAGAATGGCATAACTCTGCATTTAGAGGAAGAACCTGCAATAGTCATCAAGGTAAATCAGAAGATGGAAACCAAGAAATCAGGAGACTTAGATAACCCAGAAGTCATGAACACCACAGATAATCCACATGAAGTTATTATGAGTGAGGAGACAGCATCAGAATCAGTGAGAGCAAAGAAGTTTAAAGATGCAGCTAGTGCCGCTCAAGAAGCCTTTCAATCAGCAGCTTATGCAGCATTAGCAGCTAAAGCAGCGATCGAACTTTCAAGATCTGAATCACAGGATATTCATATAGAGGAAAAAAACTCAGATTTGGAGAGTTCTCATGAACCCAAGATTAATCCTGATGAGCTTGAAAGTTGCAGTTCAGAATATGAAGATCAAGACGCAGCAGAAAGCAATCAAGAAGAAATACCAGAAGAACCAGCCAAAGAAATTATGTTGTCTGATCCGAATTTGGATTCAGATATTAGCAATCTGAAGGAGCAATCATCATCATCTAAACCATATTCGATCAGTGAGCCAGAAAGTGACAAGTTAATCAGTAAAGAAGATGAAGTTGTAATTAAAAACAAAATTTCAGAAGAGGATATCAGAGTTGAGGAATCAAAGTAA

Coding sequence (CDS)

ATGGGAAGGAAACTGGATGCTCTACTAGGAAGGAACAGATATTTGAAGCCTTCCAAGATCAAAATCCTTGTAAACATGGCCATTTCCAGGACTGCCATTCTCAAAAACCAGCACCGTGCTCGCTGCTCCCTTGCCCGGGCCGACGTTCTTCAGCTCTTGAACCTCAGTTACCAACACCGCGCTCAGCTTAGAGTTGAGATTGTGATAAAGGAGAACAACATGATGGATGCACTTGGAATGATAGAGGATTACTGTTCCCTCCTCATAGAAAAAGTGGCTCTACTTCAAATGAACAAGGAATGCCCTGGTGAAGTTAAGGAGGCAATATCAAGTCTGATCTTTGCATCTTCAAGGTTTGGAGAGTTTCCCGAACTCCAGGAGATTCGTCGGATTTTCGAGCTGAAATTCGGGAAAGAGTTTGCTAGCAGTGCCGTTAATTTACGCAACAATTGTGGAGTTAGTCCCAAGATGATCCAAAAGTTTTCAACAAAGCAGCCCAACGCTGAAACTAAACTGAAAGTTCTAAAGGAAATTGCTTCTGAGAATGGCATAACTCTGCATTTAGAGGAAGAACCTGCAATAGTCATCAAGGTAAATCAGAAGATGGAAACCAAGAAATCAGGAGACTTAGATAACCCAGAAGTCATGAACACCACAGATAATCCACATGAAGTTATTATGAGTGAGGAGACAGCATCAGAATCAGTGAGAGCAAAGAAGTTTAAAGATGCAGCTAGTGCCGCTCAAGAAGCCTTTCAATCAGCAGCTTATGCAGCATTAGCAGCTAAAGCAGCGATCGAACTTTCAAGATCTGAATCACAGGATATTCATATAGAGGAAAAAAACTCAGATTTGGAGAGTTCTCATGAACCCAAGATTAATCCTGATGAGCTTGAAAGTTGCAGTTCAGAATATGAAGATCAAGACGCAGCAGAAAGCAATCAAGAAGAAATACCAGAAGAACCAGCCAAAGAAATTATGTTGTCTGATCCGAATTTGGATTCAGATATTAGCAATCTGAAGGAGCAATCATCATCATCTAAACCATATTCGATCAGTGAGCCAGAAAGTGACAAGTTAATCAGTAAAGAAGATGAAGTTGTAATTAAAAACAAAATTTCAGAAGAGGATATCAGAGTTGAGGAATCAAAGTAA

Protein sequence

MGRKLDALLGRNRYLKPSKIKILVNMAISRTAILKNQHRARCSLARADVLQLLNLSYQHRAQLRVEIVIKENNMMDALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRFGEFPELQEIRRIFELKFGKEFASSAVNLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIASENGITLHLEEEPAIVIKVNQKMETKKSGDLDNPEVMNTTDNPHEVIMSEETASESVRAKKFKDAASAAQEAFQSAAYAALAAKAAIELSRSESQDIHIEEKNSDLESSHEPKINPDELESCSSEYEDQDAAESNQEEIPEEPAKEIMLSDPNLDSDISNLKEQSSSSKPYSISEPESDKLISKEDEVVIKNKISEEDIRVEESK
Homology
BLAST of Csor.00g151230 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 1.1e-15
Identity = 51/163 (31.29%), Postives = 93/163 (57.06%), Query Frame = 0

Query: 19  KIKILVNMAISRTAILKNQHRARCSLARADVLQLLNLSYQHRAQLRVEIVIKENNMMDAL 78
           K+K+ + +A+SR  ILKN+        + +V +LL    +  A++RVE +I++  +++  
Sbjct: 12  KLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRDEYLIECF 71

Query: 79  GMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRFGEFPELQEIRRIFELKFGK 138
            +IE  C LL  ++ L+    E P E+KE+I +L+++S R  + PEL++I+   + K+GK
Sbjct: 72  QIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPELEQIKNQLKAKYGK 131

Query: 139 EFASSAVNLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIASE 182
              + A N   +  V+PK++ K S   P+     + L EIA +
Sbjct: 132 GLENEA-NCHCSTHVNPKIVHKLSYATPDPSIIFQTLSEIAEK 172

BLAST of Csor.00g151230 vs. ExPASy Swiss-Prot
Match: Q3ZBV1 (IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 2.4e-10
Identity = 46/172 (26.74%), Postives = 91/172 (52.91%), Query Frame = 0

Query: 15  LKPSKIKILVNMAISRTAILKNQHRARCSLARADVLQLLNLSYQHRAQLRVEIVIKENNM 74
           +K  ++++ + + I+R  +L+ +       AR ++   L      RA++RVE +I+E+ +
Sbjct: 6   IKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYL 65

Query: 75  MDALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRF-GEFPELQEIRRIFE 134
           ++A+ ++E YC LL+ +  L+Q  KE    + E++S+LI+A+ R   E  EL+ +     
Sbjct: 66  VEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLC 125

Query: 135 LKFGKEFASSAVNLRNNCG-VSPKMIQKFSTKQPNAETKLKVLKEIASENGI 185
            K+ KE+    +   N  G V+ +++ K S + P      + L EIA    +
Sbjct: 126 AKYSKEYGK--LCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNV 175

BLAST of Csor.00g151230 vs. ExPASy Swiss-Prot
Match: P53990 (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 3.1e-10
Identity = 46/171 (26.90%), Postives = 90/171 (52.63%), Query Frame = 0

Query: 16  KPSKIKILVNMAISRTAILKNQHRARCSLARADVLQLLNLSYQHRAQLRVEIVIKENNMM 75
           K  ++++ + + I+R  +L+ +       AR ++   L      RA++RVE +I+E+ ++
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 76  DALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRF-GEFPELQEIRRIFEL 135
           +A+ ++E YC LL+ +  L+Q  KE    + E++S+LI+A+ R   E  EL+ +      
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 136 KFGKEFASSAVNLRNNCG-VSPKMIQKFSTKQPNAETKLKVLKEIASENGI 185
           K+ KE+    +   N  G V+ +++ K S + P      + L EIA    +
Sbjct: 127 KYSKEYGK--LCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNV 175

BLAST of Csor.00g151230 vs. ExPASy Swiss-Prot
Match: Q9CX00 (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 3.1e-10
Identity = 46/171 (26.90%), Postives = 90/171 (52.63%), Query Frame = 0

Query: 16  KPSKIKILVNMAISRTAILKNQHRARCSLARADVLQLLNLSYQHRAQLRVEIVIKENNMM 75
           K  ++++ + + I+R  +L+ +       AR ++   L      RA++RVE +I+E+ ++
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 76  DALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRF-GEFPELQEIRRIFEL 135
           +A+ ++E YC LL+ +  L+Q  KE    + E++S+LI+A+ R   E  EL+ +      
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 136 KFGKEFASSAVNLRNNCG-VSPKMIQKFSTKQPNAETKLKVLKEIASENGI 185
           K+ KE+    +   N  G V+ +++ K S + P      + L EIA    +
Sbjct: 127 KYSKEYGK--LCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNV 175

BLAST of Csor.00g151230 vs. ExPASy Swiss-Prot
Match: Q568Z6 (IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 3.1e-10
Identity = 46/171 (26.90%), Postives = 90/171 (52.63%), Query Frame = 0

Query: 16  KPSKIKILVNMAISRTAILKNQHRARCSLARADVLQLLNLSYQHRAQLRVEIVIKENNMM 75
           K  ++++ + + I+R  +L+ +       AR ++   L      RA++RVE +I+E+ ++
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 76  DALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRF-GEFPELQEIRRIFEL 135
           +A+ ++E YC LL+ +  L+Q  KE    + E++S+LI+A+ R   E  EL+ +      
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 136 KFGKEFASSAVNLRNNCG-VSPKMIQKFSTKQPNAETKLKVLKEIASENGI 185
           K+ KE+    +   N  G V+ +++ K S + P      + L EIA    +
Sbjct: 127 KYSKEYGK--LCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNV 175

BLAST of Csor.00g151230 vs. NCBI nr
Match: KAG6581175.1 (IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 699 bits (1804), Expect = 9.95e-253
Identity = 384/384 (100.00%), Postives = 384/384 (100.00%), Query Frame = 0

Query: 1   MGRKLDALLGRNRYLKPSKIKILVNMAISRTAILKNQHRARCSLARADVLQLLNLSYQHR 60
           MGRKLDALLGRNRYLKPSKIKILVNMAISRTAILKNQHRARCSLARADVLQLLNLSYQHR
Sbjct: 1   MGRKLDALLGRNRYLKPSKIKILVNMAISRTAILKNQHRARCSLARADVLQLLNLSYQHR 60

Query: 61  AQLRVEIVIKENNMMDALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRFG 120
           AQLRVEIVIKENNMMDALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRFG
Sbjct: 61  AQLRVEIVIKENNMMDALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRFG 120

Query: 121 EFPELQEIRRIFELKFGKEFASSAVNLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS 180
           EFPELQEIRRIFELKFGKEFASSAVNLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS
Sbjct: 121 EFPELQEIRRIFELKFGKEFASSAVNLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS 180

Query: 181 ENGITLHLEEEPAIVIKVNQKMETKKSGDLDNPEVMNTTDNPHEVIMSEETASESVRAKK 240
           ENGITLHLEEEPAIVIKVNQKMETKKSGDLDNPEVMNTTDNPHEVIMSEETASESVRAKK
Sbjct: 181 ENGITLHLEEEPAIVIKVNQKMETKKSGDLDNPEVMNTTDNPHEVIMSEETASESVRAKK 240

Query: 241 FKDAASAAQEAFQSAAYAALAAKAAIELSRSESQDIHIEEKNSDLESSHEPKINPDELES 300
           FKDAASAAQEAFQSAAYAALAAKAAIELSRSESQDIHIEEKNSDLESSHEPKINPDELES
Sbjct: 241 FKDAASAAQEAFQSAAYAALAAKAAIELSRSESQDIHIEEKNSDLESSHEPKINPDELES 300

Query: 301 CSSEYEDQDAAESNQEEIPEEPAKEIMLSDPNLDSDISNLKEQSSSSKPYSISEPESDKL 360
           CSSEYEDQDAAESNQEEIPEEPAKEIMLSDPNLDSDISNLKEQSSSSKPYSISEPESDKL
Sbjct: 301 CSSEYEDQDAAESNQEEIPEEPAKEIMLSDPNLDSDISNLKEQSSSSKPYSISEPESDKL 360

Query: 361 ISKEDEVVIKNKISEEDIRVEESK 384
           ISKEDEVVIKNKISEEDIRVEESK
Sbjct: 361 ISKEDEVVIKNKISEEDIRVEESK 384

BLAST of Csor.00g151230 vs. NCBI nr
Match: XP_022934804.1 (uncharacterized protein LOC111441867 [Cucurbita moschata])

HSP 1 Score: 676 bits (1744), Expect = 1.49e-243
Identity = 374/386 (96.89%), Postives = 378/386 (97.93%), Query Frame = 0

Query: 1   MGRKLDALLGRNRYLKPSKIKILVNMAISRTAILKNQHRARCSLARADVLQLLNLSYQHR 60
           MGRKLDALLGRNRYLKPSKIKILVNMAISRTAILKNQHRARCSLARADVLQLLNLSYQHR
Sbjct: 1   MGRKLDALLGRNRYLKPSKIKILVNMAISRTAILKNQHRARCSLARADVLQLLNLSYQHR 60

Query: 61  AQLRVEIVIKENNMMDALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRFG 120
           AQLRVEIVIKENNMMDALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRFG
Sbjct: 61  AQLRVEIVIKENNMMDALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRFG 120

Query: 121 EFPELQEIRRIFELKFGKEFASSAVNLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS 180
           EFPELQEIRRIFELKFGKEFASSAVNLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS
Sbjct: 121 EFPELQEIRRIFELKFGKEFASSAVNLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS 180

Query: 181 ENGITLHLEEEPAIVIKVNQKMETKKSGDLDNPEVMNTTDNPHEVIMSEETASESVRAKK 240
           ENGITLHLEEEPAIVIKVNQKMETKKS DLDNPEVMNTTDNPHEVIMSEE ASESVR KK
Sbjct: 181 ENGITLHLEEEPAIVIKVNQKMETKKSADLDNPEVMNTTDNPHEVIMSEEIASESVREKK 240

Query: 241 FKDAASAAQEAFQSAAYAALAAKAAIELSRSESQDIHIEEKNSDLESSHEPKINPDELES 300
           FKDAASAA+EAFQSAAYAALAAKAAIELSRSESQDIHIEE+NSDLESSHEPKINPDELES
Sbjct: 241 FKDAASAAKEAFQSAAYAALAAKAAIELSRSESQDIHIEERNSDLESSHEPKINPDELES 300

Query: 301 CSSEYEDQDAAESNQEEIPEEPAKEIMLSDPNLDSDISNLKEQSSSSKPYSIS--EPESD 360
           CSSEYEDQDAAESNQEE PEEPAKEIMLSDPNLDSD+SNLKEQSSSSKP SIS  EPESD
Sbjct: 301 CSSEYEDQDAAESNQEEFPEEPAKEIMLSDPNLDSDVSNLKEQSSSSKPNSISHNEPESD 360

Query: 361 KLISKEDEVVIKNKISEEDIRVEESK 384
           KL+SKEDEVV KNKISEEDIRVEESK
Sbjct: 361 KLMSKEDEVVSKNKISEEDIRVEESK 386

BLAST of Csor.00g151230 vs. NCBI nr
Match: XP_022983568.1 (uncharacterized protein LOC111482134 [Cucurbita maxima])

HSP 1 Score: 672 bits (1733), Expect = 7.04e-242
Identity = 370/386 (95.85%), Postives = 378/386 (97.93%), Query Frame = 0

Query: 1   MGRKLDALLGRNRYLKPSKIKILVNMAISRTAILKNQHRARCSLARADVLQLLNLSYQHR 60
           MGRKLDALLGRNRYLKPSKIKIL NMAISRTAILKNQHRARCSLARADVLQLLNLSYQHR
Sbjct: 1   MGRKLDALLGRNRYLKPSKIKILANMAISRTAILKNQHRARCSLARADVLQLLNLSYQHR 60

Query: 61  AQLRVEIVIKENNMMDALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRFG 120
           AQLRVEIVIKENNMMDALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRFG
Sbjct: 61  AQLRVEIVIKENNMMDALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRFG 120

Query: 121 EFPELQEIRRIFELKFGKEFASSAVNLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS 180
           EFPELQEIRRIFELKFGKEFA SA++LRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS
Sbjct: 121 EFPELQEIRRIFELKFGKEFAGSAIDLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS 180

Query: 181 ENGITLHLEEEPAIVIKVNQKMETKKSGDLDNPEVMNTTDNPHEVIMSEETASESVRAKK 240
           ENGITLHLEEEPAIVIKVNQK+ETKKS DLDNPEV+NTTDNPHEVIMSEETASESVRAKK
Sbjct: 181 ENGITLHLEEEPAIVIKVNQKLETKKSADLDNPEVINTTDNPHEVIMSEETASESVRAKK 240

Query: 241 FKDAASAAQEAFQSAAYAALAAKAAIELSRSESQDIHIEEKNSDLESSHEPKINPDELES 300
           FKDAASAAQEAFQSAAYAALAAKAAIELSRSESQDIHIEE+NSDLESSHEPKINPDELES
Sbjct: 241 FKDAASAAQEAFQSAAYAALAAKAAIELSRSESQDIHIEERNSDLESSHEPKINPDELES 300

Query: 301 CSSEYEDQDAAESNQEEIPEEPAKEIMLSDPNLDSDISNLKEQSSSSKPYSIS--EPESD 360
           CSSEYEDQDAAESNQEE+PEEPAKEIMLSDPNLDSDISN+KE+SSSSKP SIS  EPESD
Sbjct: 301 CSSEYEDQDAAESNQEELPEEPAKEIMLSDPNLDSDISNMKEKSSSSKPNSISHNEPESD 360

Query: 361 KLISKEDEVVIKNKISEEDIRVEESK 384
           KL SKEDEVV KNKISEEDIRVEESK
Sbjct: 361 KLTSKEDEVVSKNKISEEDIRVEESK 386

BLAST of Csor.00g151230 vs. NCBI nr
Match: XP_023527290.1 (uncharacterized protein LOC111790567 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 662 bits (1708), Expect = 8.20e-238
Identity = 370/402 (92.04%), Postives = 377/402 (93.78%), Query Frame = 0

Query: 1   MGRKLDALLGRNRYLKPSKIKILVNMAISRTAILKNQHRARCSLARADVLQLLNLSYQHR 60
           MGRKLDALLGRNRYLKPSKIKILVNMAISRTAILKNQHRARCSLARADVLQLLNLSYQHR
Sbjct: 1   MGRKLDALLGRNRYLKPSKIKILVNMAISRTAILKNQHRARCSLARADVLQLLNLSYQHR 60

Query: 61  AQLRVEIVIKENNMMDALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRFG 120
           AQLRVEIVIKENNMMDALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRFG
Sbjct: 61  AQLRVEIVIKENNMMDALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRFG 120

Query: 121 EFPELQEIRRIFELKFGKEFASSAVNLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS 180
           EFPELQEIRRIFELKFGKEF SSAV+LRNNCGVS  MIQKFSTKQPNAETKLKVLKEIAS
Sbjct: 121 EFPELQEIRRIFELKFGKEFTSSAVDLRNNCGVSHTMIQKFSTKQPNAETKLKVLKEIAS 180

Query: 181 ENGITLHLEEEPAIVIKVNQKMETKKSGDLDNPEVMNTTDNPHEVIMSEETASESVRAKK 240
           ENG+TLHLEEEPAIVIKVNQK+ETKKS +LDNPEV+NTTDNPHEVIMSEETASESVRAKK
Sbjct: 181 ENGLTLHLEEEPAIVIKVNQKLETKKSAELDNPEVINTTDNPHEVIMSEETASESVRAKK 240

Query: 241 FKDAASAAQEAFQSAAYAALAAKAAIELSRSESQDIHIEEKNSDLESSHE---------- 300
           FKDAASAAQEAFQSAAYAALAAKAAIELSRSESQDIHIEEKNSDLESSHE          
Sbjct: 241 FKDAASAAQEAFQSAAYAALAAKAAIELSRSESQDIHIEEKNSDLESSHESQDIHIEEKN 300

Query: 301 --------PKINPDELESCSSEYEDQDAAESNQEEIPEEPAKEIMLSDPNLDSDISNLKE 360
                   PKINPDELESCSSEYEDQDAAESNQEE+PEEPAKEIMLSDPNLDSDISNLKE
Sbjct: 301 SDLESSHEPKINPDELESCSSEYEDQDAAESNQEELPEEPAKEIMLSDPNLDSDISNLKE 360

Query: 361 QSSSSKPYSISEPESDKLISKEDEVVIKNKISEEDIRVEESK 384
           QSSSSKP SI+EPESDKLISKEDEV  KNKISEEDIRVEESK
Sbjct: 361 QSSSSKPNSINEPESDKLISKEDEVASKNKISEEDIRVEESK 402

BLAST of Csor.00g151230 vs. NCBI nr
Match: XP_038875575.1 (uncharacterized protein LOC120067980 isoform X2 [Benincasa hispida])

HSP 1 Score: 536 bits (1380), Expect = 1.26e-187
Identity = 317/420 (75.48%), Postives = 344/420 (81.90%), Query Frame = 0

Query: 1   MGRKLDALLGRNRYLKPSKIKILVNMAISRTAILKNQHRARCSLARADVLQLLNLSYQHR 60
           MGRKLDALLGRNRYLKPSK K L NMAISRT+ILKNQHRAR SLARADVLQLLNLSYQHR
Sbjct: 1   MGRKLDALLGRNRYLKPSKFKTLANMAISRTSILKNQHRARSSLARADVLQLLNLSYQHR 60

Query: 61  AQLRVEIVIKENNMMDALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRFG 120
           AQLRVEIVIKENNM+DAL MIEDYC LLIEKVALLQMNKECPGEVKEAISSLIFA+SR G
Sbjct: 61  AQLRVEIVIKENNMLDALEMIEDYCCLLIEKVALLQMNKECPGEVKEAISSLIFAASRCG 120

Query: 121 EFPELQEIRRIFELKFGKEFASSAVNLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS 180
           EFPELQEIRRI ELKFG EFASSAVNLRNNCGVSPKMIQKFSTKQP+AETKLKVLKEIAS
Sbjct: 121 EFPELQEIRRILELKFGTEFASSAVNLRNNCGVSPKMIQKFSTKQPSAETKLKVLKEIAS 180

Query: 181 ENGITLHLEEEPAIVIK--VNQKMETKKSGDLDNPEVMNTTDNPHEVIMSEETASESVRA 240
           ENGIT HLEEEPAI+IK  +NQK+E K++ DLDNPEV+NTTDN HEVI S+E+A ESV+A
Sbjct: 181 ENGITQHLEEEPAIIIKEKLNQKLEAKEAADLDNPEVINTTDNLHEVITSDESALESVKA 240

Query: 241 KKFKDAASAAQEAFQSAAYAALAAKAAIELSRSESQDI--------HIE--EKNSDLESS 300
           KKFKD ASA QEAFQSAAYAALAAKAAI LS+SE+QDI        H++  EK+SDL+SS
Sbjct: 241 KKFKDVASATQEAFQSAAYAALAAKAAITLSKSEAQDIDSDHEDGSHLQTDEKSSDLDSS 300

Query: 301 HEPKIN------------------------PDELESCSSEYEDQDAAESNQEEIPEEPAK 360
           HEPK+N                        P ELESCSSEYED D AESNQ E+P+E AK
Sbjct: 301 HEPKLNTKEASIPSSKIDYSDDRFSFEKICPAELESCSSEYEDADMAESNQIELPKETAK 360

Query: 361 EIMLSDPNLDSDISNLKEQSSSSKPYSISEPESDKLISKEDEVVIKNKISEEDIRVEESK 384
           EIM S   LDSDISN+KEQSS       +EPE DK+I KE+EV  KN+ISE DIRV ESK
Sbjct: 361 EIMSSVLILDSDISNMKEQSSKLNSIFYNEPEGDKIIGKENEVASKNEISE-DIRVHESK 419

BLAST of Csor.00g151230 vs. ExPASy TrEMBL
Match: A0A6J1F3M1 (uncharacterized protein LOC111441867 OS=Cucurbita moschata OX=3662 GN=LOC111441867 PE=3 SV=1)

HSP 1 Score: 676 bits (1744), Expect = 7.19e-244
Identity = 374/386 (96.89%), Postives = 378/386 (97.93%), Query Frame = 0

Query: 1   MGRKLDALLGRNRYLKPSKIKILVNMAISRTAILKNQHRARCSLARADVLQLLNLSYQHR 60
           MGRKLDALLGRNRYLKPSKIKILVNMAISRTAILKNQHRARCSLARADVLQLLNLSYQHR
Sbjct: 1   MGRKLDALLGRNRYLKPSKIKILVNMAISRTAILKNQHRARCSLARADVLQLLNLSYQHR 60

Query: 61  AQLRVEIVIKENNMMDALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRFG 120
           AQLRVEIVIKENNMMDALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRFG
Sbjct: 61  AQLRVEIVIKENNMMDALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRFG 120

Query: 121 EFPELQEIRRIFELKFGKEFASSAVNLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS 180
           EFPELQEIRRIFELKFGKEFASSAVNLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS
Sbjct: 121 EFPELQEIRRIFELKFGKEFASSAVNLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS 180

Query: 181 ENGITLHLEEEPAIVIKVNQKMETKKSGDLDNPEVMNTTDNPHEVIMSEETASESVRAKK 240
           ENGITLHLEEEPAIVIKVNQKMETKKS DLDNPEVMNTTDNPHEVIMSEE ASESVR KK
Sbjct: 181 ENGITLHLEEEPAIVIKVNQKMETKKSADLDNPEVMNTTDNPHEVIMSEEIASESVREKK 240

Query: 241 FKDAASAAQEAFQSAAYAALAAKAAIELSRSESQDIHIEEKNSDLESSHEPKINPDELES 300
           FKDAASAA+EAFQSAAYAALAAKAAIELSRSESQDIHIEE+NSDLESSHEPKINPDELES
Sbjct: 241 FKDAASAAKEAFQSAAYAALAAKAAIELSRSESQDIHIEERNSDLESSHEPKINPDELES 300

Query: 301 CSSEYEDQDAAESNQEEIPEEPAKEIMLSDPNLDSDISNLKEQSSSSKPYSIS--EPESD 360
           CSSEYEDQDAAESNQEE PEEPAKEIMLSDPNLDSD+SNLKEQSSSSKP SIS  EPESD
Sbjct: 301 CSSEYEDQDAAESNQEEFPEEPAKEIMLSDPNLDSDVSNLKEQSSSSKPNSISHNEPESD 360

Query: 361 KLISKEDEVVIKNKISEEDIRVEESK 384
           KL+SKEDEVV KNKISEEDIRVEESK
Sbjct: 361 KLMSKEDEVVSKNKISEEDIRVEESK 386

BLAST of Csor.00g151230 vs. ExPASy TrEMBL
Match: A0A6J1J838 (uncharacterized protein LOC111482134 OS=Cucurbita maxima OX=3661 GN=LOC111482134 PE=3 SV=1)

HSP 1 Score: 672 bits (1733), Expect = 3.41e-242
Identity = 370/386 (95.85%), Postives = 378/386 (97.93%), Query Frame = 0

Query: 1   MGRKLDALLGRNRYLKPSKIKILVNMAISRTAILKNQHRARCSLARADVLQLLNLSYQHR 60
           MGRKLDALLGRNRYLKPSKIKIL NMAISRTAILKNQHRARCSLARADVLQLLNLSYQHR
Sbjct: 1   MGRKLDALLGRNRYLKPSKIKILANMAISRTAILKNQHRARCSLARADVLQLLNLSYQHR 60

Query: 61  AQLRVEIVIKENNMMDALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRFG 120
           AQLRVEIVIKENNMMDALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRFG
Sbjct: 61  AQLRVEIVIKENNMMDALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRFG 120

Query: 121 EFPELQEIRRIFELKFGKEFASSAVNLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS 180
           EFPELQEIRRIFELKFGKEFA SA++LRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS
Sbjct: 121 EFPELQEIRRIFELKFGKEFAGSAIDLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS 180

Query: 181 ENGITLHLEEEPAIVIKVNQKMETKKSGDLDNPEVMNTTDNPHEVIMSEETASESVRAKK 240
           ENGITLHLEEEPAIVIKVNQK+ETKKS DLDNPEV+NTTDNPHEVIMSEETASESVRAKK
Sbjct: 181 ENGITLHLEEEPAIVIKVNQKLETKKSADLDNPEVINTTDNPHEVIMSEETASESVRAKK 240

Query: 241 FKDAASAAQEAFQSAAYAALAAKAAIELSRSESQDIHIEEKNSDLESSHEPKINPDELES 300
           FKDAASAAQEAFQSAAYAALAAKAAIELSRSESQDIHIEE+NSDLESSHEPKINPDELES
Sbjct: 241 FKDAASAAQEAFQSAAYAALAAKAAIELSRSESQDIHIEERNSDLESSHEPKINPDELES 300

Query: 301 CSSEYEDQDAAESNQEEIPEEPAKEIMLSDPNLDSDISNLKEQSSSSKPYSIS--EPESD 360
           CSSEYEDQDAAESNQEE+PEEPAKEIMLSDPNLDSDISN+KE+SSSSKP SIS  EPESD
Sbjct: 301 CSSEYEDQDAAESNQEELPEEPAKEIMLSDPNLDSDISNMKEKSSSSKPNSISHNEPESD 360

Query: 361 KLISKEDEVVIKNKISEEDIRVEESK 384
           KL SKEDEVV KNKISEEDIRVEESK
Sbjct: 361 KLTSKEDEVVSKNKISEEDIRVEESK 386

BLAST of Csor.00g151230 vs. ExPASy TrEMBL
Match: A0A0A0L6Y2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G271380 PE=3 SV=1)

HSP 1 Score: 494 bits (1272), Expect = 1.58e-171
Identity = 297/422 (70.38%), Postives = 333/422 (78.91%), Query Frame = 0

Query: 1   MGRKLDALLGRNRYLKPSKIKILVNMAISRTAILKNQHRARCSLARADVLQLLNLSYQHR 60
           MGRKLDALLGRNRYLK SK K L NMAISRT+ILKN HRARCSLARADVLQLLNLSYQHR
Sbjct: 1   MGRKLDALLGRNRYLKASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHR 60

Query: 61  AQLRVEIVIKENNMMDALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRFG 120
           AQLRVEIVIKENNM+DALGMIEDYC LL+EKVALLQMNKECPGEVKEAISSLIFA+SR G
Sbjct: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRCG 120

Query: 121 EFPELQEIRRIFELKFGKEFASSAVNLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS 180
           EFPELQEIRRIFELKFG EFASSA++LRNNCGVS KMIQKFSTKQP+AETKLKVLKEIAS
Sbjct: 121 EFPELQEIRRIFELKFGAEFASSAIDLRNNCGVSTKMIQKFSTKQPSAETKLKVLKEIAS 180

Query: 181 ENGITLHLEEEPAIVIKV--NQKMETKKSGDLDNPEVMNTTDNPHEVIMSEETASESVRA 240
           ENGI LHLE+E  I+IK   NQK+E KK  DL NPEV NTTD+ HEVI SE+ ASE V++
Sbjct: 181 ENGIPLHLEQESPIIIKAKQNQKLEIKKEADLYNPEVTNTTDDLHEVITSEKMASELVKS 240

Query: 241 KKFKDAASAAQEAFQSAAYAALAAKAAIELSRSESQDI--------HIEE--KNSDLESS 300
           KKFKD ASA +E FQSAAYAALAAKAAI LS+S+SQDI        H+++  K SDL SS
Sbjct: 241 KKFKDVASATEEPFQSAAYAALAAKAAINLSKSKSQDIDSDHEDGSHLQKNGKGSDLNSS 300

Query: 301 HEPKIN------------------------PDELESCSSEYEDQDAAESNQEEIPEEPAK 360
           +EPK+N                        P ELESCSS+YED D  E+NQEE+P+EPAK
Sbjct: 301 YEPKLNTKEERISSCKIDYSDDRFSFEKICPVELESCSSQYEDADMEETNQEELPKEPAK 360

Query: 361 EIMLSDPNLDSDISNLKEQSSSSKPYSI--SEPESDKLISKEDEVVIKNKISEEDIRVEE 384
           E   S    DSD++N+KEQSS  KP SI  +E + DK+ + ++EV    +ISEEDIRV E
Sbjct: 361 ES-TSFALKDSDVNNMKEQSS--KPNSIFYNESDGDKITNTDNEVASGKEISEEDIRVHE 419

BLAST of Csor.00g151230 vs. ExPASy TrEMBL
Match: A0A5D3CIE3 (Putative Regulator of Vps4 activity in the MVB pathway protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold828G00600 PE=3 SV=1)

HSP 1 Score: 482 bits (1240), Expect = 9.26e-167
Identity = 288/420 (68.57%), Postives = 323/420 (76.90%), Query Frame = 0

Query: 1   MGRKLDALLGRNRYLKPSKIKILVNMAISRTAILKNQHRARCSLARADVLQLLNLSYQHR 60
           MGRKLDALLGRNRYLKPSK K L NMAISRT+ILKNQHRARCSLARADVLQLLNL YQHR
Sbjct: 1   MGRKLDALLGRNRYLKPSKFKTLANMAISRTSILKNQHRARCSLARADVLQLLNLGYQHR 60

Query: 61  AQLRVEIVIKENNMMDALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRFG 120
           AQLRVEIVIKENNM+DALGMIEDYC LL+EKVAL QMNKECPGEVKEAISSLIFA+SR G
Sbjct: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLVEKVALFQMNKECPGEVKEAISSLIFAASRCG 120

Query: 121 EFPELQEIRRIFELKFGKEFASSAVNLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS 180
           EFPELQEIRRIFELKFG EFASSA++LRNNCGVSPKMIQKFSTKQP AETKLKVLKEIAS
Sbjct: 121 EFPELQEIRRIFELKFGTEFASSAIDLRNNCGVSPKMIQKFSTKQPTAETKLKVLKEIAS 180

Query: 181 ENGITLHLEEEPAIVIK--VNQKMETKKSGDLDNPEVMNTTDNPHEVIMSEETASESVRA 240
           ENGI L+LEEEP I+IK  +NQK+E KK  DL NPEV NTTD+ HEVI SE+ ASE V+A
Sbjct: 181 ENGIPLNLEEEPPIIIKEKLNQKLEVKKEADLYNPEVTNTTDDLHEVITSEKLASELVKA 240

Query: 241 KKFKDAASAAQEAFQSAAYAALAAKAAIELSRSESQDI--------HIEE--KNSDLESS 300
           KKFKD ASAA+EAFQSAA A       I   +SESQDI        H+++  K+S+L SS
Sbjct: 241 KKFKDVASAAEEAFQSAAKA-------INFLKSESQDIDSDHEDGSHLQKNGKSSELNSS 300

Query: 301 HEPKINPDE------------------------LESCSSEYEDQDAAESNQEEIPEEPAK 360
           +E K+N  E                        LESCSS+YED D  E+NQEE+P+EPA 
Sbjct: 301 YESKLNTKEASIPSSKIDYSDDRFGFEKICPVGLESCSSQYEDADMEETNQEELPKEPAI 360

Query: 361 EIMLSDPNLDSDISNLKEQSSSSKPYSISEPESDKLISKEDEVVIKNKISEEDIRVEESK 384
           E   S  NL SDI+N KEQSS       +E + DK+ISK++EV    +ISEE+I+V ESK
Sbjct: 361 ESTSSVLNLSSDINNTKEQSSEPNSTFYNESDGDKIISKDNEVASGKEISEENIKVHESK 413

BLAST of Csor.00g151230 vs. ExPASy TrEMBL
Match: A0A1S3CEY6 (uncharacterized protein LOC103500154 OS=Cucumis melo OX=3656 GN=LOC103500154 PE=3 SV=1)

HSP 1 Score: 478 bits (1231), Expect = 2.16e-165
Identity = 286/420 (68.10%), Postives = 322/420 (76.67%), Query Frame = 0

Query: 1   MGRKLDALLGRNRYLKPSKIKILVNMAISRTAILKNQHRARCSLARADVLQLLNLSYQHR 60
           MGRKLDALLGRNRYLKPSK K L NMAISRT+ILKNQHRARCSLARADVLQLLNL YQHR
Sbjct: 1   MGRKLDALLGRNRYLKPSKFKTLANMAISRTSILKNQHRARCSLARADVLQLLNLGYQHR 60

Query: 61  AQLRVEIVIKENNMMDALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRFG 120
           AQLRVEIVIKENNM+DALGMIEDYC LL+EKVAL QMNKECPGEVKEAISSLIFA+SR G
Sbjct: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLVEKVALFQMNKECPGEVKEAISSLIFAASRCG 120

Query: 121 EFPELQEIRRIFELKFGKEFASSAVNLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS 180
           EFPELQEIRRIFELKFG EFASSA++LRNNCGVSPKMIQKFSTKQP AETKLKVLKEIAS
Sbjct: 121 EFPELQEIRRIFELKFGTEFASSAIDLRNNCGVSPKMIQKFSTKQPTAETKLKVLKEIAS 180

Query: 181 ENGITLHLEEEPAIVIK--VNQKMETKKSGDLDNPEVMNTTDNPHEVIMSEETASESVRA 240
           ENGI L+LEEEP I++K  +NQK+E KK  DL NPEV NTTD+ HEVI SE+ ASE V+A
Sbjct: 181 ENGIPLNLEEEPPIIVKEKLNQKLEVKKEADLYNPEVTNTTDDLHEVITSEKLASELVKA 240

Query: 241 KKFKDAASAAQEAFQSAAYAALAAKAAIELSRSESQDI--------HIEE--KNSDLESS 300
           KKFKD ASAA+EAFQSAA A       I   +SESQDI        H+++  K+S+L SS
Sbjct: 241 KKFKDVASAAEEAFQSAAKA-------INFLKSESQDIDSDHEDGSHLQKNGKSSELNSS 300

Query: 301 HEPKINPDE------------------------LESCSSEYEDQDAAESNQEEIPEEPAK 360
           +E K+N  E                        LESCSS+YED D  E+NQEE+P+EPA 
Sbjct: 301 YESKLNTKEASIPSSKIDYSDDRFSFEKICPVGLESCSSQYEDADMEETNQEELPKEPAI 360

Query: 361 EIMLSDPNLDSDISNLKEQSSSSKPYSISEPESDKLISKEDEVVIKNKISEEDIRVEESK 384
           E   S  NL SDI+N KEQSS       +E + DK+ISK++EV    +ISEE+I+V E K
Sbjct: 361 ESTSSVLNLCSDINNTKEQSSEPNSTFYNESDGDKIISKDNEVASGKEISEENIKVHEPK 413

BLAST of Csor.00g151230 vs. TAIR 10
Match: AT1G13340.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 189.5 bits (480), Expect = 5.0e-48
Identity = 144/386 (37.31%), Postives = 220/386 (56.99%), Query Frame = 0

Query: 1   MGRKLDALLGRNRYLKPSKIKILVNMAISRTAILKNQHRARCSLARADVLQLLNLSYQHR 60
           MG+KLDALLGR+   K +K K L+ +A++R +ILKNQ +AR S A +DV +LL L     
Sbjct: 1   MGKKLDALLGRS--FKTNKFKSLITLALTRLSILKNQRQARLSQAISDVTELLKLGQHEH 60

Query: 61  AQLRVEIVIKENNMMDALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRFG 120
           A  RV+ V+K+ N +D L  I  Y +L ++++ L + N++CP E+ EA+S L+FA+SR G
Sbjct: 61  AYHRVDQVVKDQNTLDVLFFIHGYFTLCLDRIHLFEHNRDCPEELLEAVSGLLFAASRIG 120

Query: 121 EFPELQEIRRIFELKFGKEFASSAVNLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS 180
           EFPELQEIR +   +FGK+ A+ ++ LR+NCGV PK+IQK ST+ P  E ++K LKEIA+
Sbjct: 121 EFPELQEIRNVLISRFGKDLAARSIELRSNCGVDPKIIQKLSTRPPPKEVRMKALKEIAA 180

Query: 181 ENGITLHLEEEPAIVIKVNQKMETKKSGDLDNPEVMNTTDNPHEVIMSEETASESVR--A 240
           EN I L L++        + +  T   G  D  +   T+ +           S+SV+   
Sbjct: 181 ENNIVLKLDQ-----ASTSTEGTTNMQGTSDVSKTKLTSKDGRG---EGYGLSDSVKRGK 240

Query: 241 KKFKDAASAAQEAFQSAAYAALAAKAAIELSRSESQDIHIEEKNSDLESSHEPKINPDEL 300
           KK+KD A AAQ AF+SAA+AA AA+AA+ELS+   +  H    N   E+S     +  E 
Sbjct: 241 KKYKDVADAAQAAFESAAHAAEAAQAAVELSQFSPRG-HDSPGNIGGENS----FHGSEN 300

Query: 301 ESCSSEYEDQDAAESNQEEIPEEPAKEIMLSDPNLDSDISNLKEQSSSSKPYSISEPESD 360
           +    E E  D +   + ++  E  + +  S+  +D  + + +E         +   E D
Sbjct: 301 KESEQEQEGNDDSSEGEGDVMSESKRSMSDSEDIIDVPVMSFRED-------PVKLLEKD 360

Query: 361 KLISKEDEVVIKNKISEEDIRVEESK 385
            +I   +E   + + S E I + +SK
Sbjct: 361 TIIYDSEE---ETQPSVETITISKSK 361

BLAST of Csor.00g151230 vs. TAIR 10
Match: AT1G34220.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 127.5 bits (319), Expect = 2.3e-29
Identity = 66/182 (36.26%), Postives = 117/182 (64.29%), Query Frame = 0

Query: 12  NRYLKPSKIKILVNMAISRTAILKNQHRARCSLARADVLQLLNLSYQHRAQLRVEIVIKE 71
           N+  K +K K L+ + I R  +++N+  A+    R ++ +LL    +  A++RVE +I+E
Sbjct: 9   NKGFKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVEHIIRE 68

Query: 72  NNMMDALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRFGEFPELQEIRRI 131
             MM A  ++E +C L+  ++ +++  +ECP ++KEAISS+ FA+ R  +  ELQ+++ +
Sbjct: 69  EKMMAAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQQVQIL 128

Query: 132 FELKFGKEFASSAVNLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIASENGITLHLEEE 191
           F  K+GKEF ++A  L+ + GV+ K+++  S + P+ ETKLK+LKEIA E+     L+ +
Sbjct: 129 FVSKYGKEFVAAASELKPDSGVNRKLVELLSVRAPSPETKLKLLKEIAEEH----ELDWD 186

Query: 192 PA 194
           PA
Sbjct: 189 PA 186

BLAST of Csor.00g151230 vs. TAIR 10
Match: AT4G29440.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 113.6 bits (283), Expect = 3.5e-25
Identity = 110/398 (27.64%), Postives = 177/398 (44.47%), Query Frame = 0

Query: 12  NRYLKPSKIKILVNMAISRTAILKNQHRARCSLARADVLQLLNLSYQHRAQLRVEIVIKE 71
           +R  KP+K KI + MA SR  ILKN+   +    R ++  LL       A++RVE V++E
Sbjct: 6   HRSFKPAKCKIALQMAASRLKILKNKKDTQIKQLRRELAHLLESGQTQTAKIRVEHVVRE 65

Query: 72  NNMMDALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRFGEFPELQEIRRI 131
              + A  ++  YC LL+ ++ ++   K CP ++KEA++S+++AS R  +  EL +I + 
Sbjct: 66  EKTVAAYELVGIYCELLVARLGVIDSQKTCPNDLKEAVASVLYASQRLTDVGELSDIVKH 125

Query: 132 FELKFGKEFASSAVNLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIASENGITLHLE-- 191
           F  K+GK+F S+A+ L+ + GVS  +++K S K P+  TK+K+L EIA+++ +T   E  
Sbjct: 126 FSAKYGKDFVSAAIGLQPDSGVSRLLVEKLSVKAPDGPTKIKILTEIATQHNVTWEAESL 185

Query: 192 ------------------EEPAIVIK-------------------VNQKMETKKSGDLDN 251
                              +PA  IK                   VN    +  +    +
Sbjct: 186 VESDPKETMSASGASSSVSQPATGIKSESSRIQNNQPPVFQAAATVNVSQNSYATDGRSS 245

Query: 252 PEVMNTTDN----PHEVIMSEETASESVRAK-----------------KFKDAASAAQEA 311
             + +T  N    P       + + + V  +                 KF DA SAA+ A
Sbjct: 246 SRMTSTDFNVGKTPDHYHQDPKPSGDRVDGREHRDHNPGHGDTSPFETKFVDATSAARAA 305

Query: 312 FQSAAYAALAAKAAIELSRSESQDIHIEEKNSDLESSHEP-KINPDELESCSSEYEDQDA 349
            +SA  A+ AA+ A ELS  E   +      S   SS+E  + NP    + SS  +    
Sbjct: 306 AESAERASFAARRAAELSSKERMMMMQNSTESRNSSSYENLRSNPPHSRTSSSNMQGGGF 365

BLAST of Csor.00g151230 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 112.1 bits (279), Expect = 1.0e-24
Identity = 108/380 (28.42%), Postives = 175/380 (46.05%), Query Frame = 0

Query: 13  RYLKPSKIKILVNMAISRTAILKNQHRARCSLARADVLQLLNLSYQHRAQLRVEIVIKEN 72
           R    SK K    MA++R  +++N+        R D+  LL       A++RVE VI+E 
Sbjct: 24  RGFNSSKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHVIREQ 83

Query: 73  NMMDALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRFGEFPELQEIRRIF 132
           N+  A  +IE +C L++ ++ ++   K+CP ++KE I+SLIFA+ R  E PEL ++R IF
Sbjct: 84  NIQAANEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDLRDIF 143

Query: 133 ELKFGKEFASSAVNLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIASENGITLHLEEEP 192
             K+GK+F S+A +LR +CGV+  +I K S + P  E KLK++KEIA E  +     E  
Sbjct: 144 AKKYGKDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAKEFQVDWDTTETE 203

Query: 193 AIVIKVNQ------KMETKKSGDLDNPEVMNTTDNPHEVIMSEETASESVRAKKFKDAAS 252
             ++K  +      +     S    N   +N   +P + +    T+S S+    + D  S
Sbjct: 204 QELLKPQEESIDGPRKFVSASSLPVNRAAINEPIDPTKAV-PRSTSSMSINT-HYHDTES 263

Query: 253 AAQEAFQSAAYAALAAKAAI-----------ELSRSESQDIHIEEKNSDLESSHEPKINP 312
           AA+ A + A  A  AA+ A            E S S     H ++        H P    
Sbjct: 264 AAEAATELAKQAVAAAQVASLLATRRDSSNKEFSVSSDHSTHQKDSQYMDHHHHHPGSRR 323

Query: 313 DELESCSSEYEDQDAAES---------NQEEIPEEPAKEIMLSDPNLDSDISNLKEQSSS 367
              +S +S Y  +  AE+         N   I E   +E      N +++      +  S
Sbjct: 324 QSRDSETSSYYAKPGAENRGMGRRHSYNNPGINESDYEE---EYTNTEAEAKETMRRRHS 383

BLAST of Csor.00g151230 vs. TAIR 10
Match: AT2G19710.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 109.4 bits (272), Expect = 6.6e-24
Identity = 115/453 (25.39%), Postives = 198/453 (43.71%), Query Frame = 0

Query: 13  RYLKPSKIKILVNMAISRTAILKNQHRARCSLARADVLQLLNLSYQHRAQLRVEIVIKEN 72
           R  KP+K K  + MA SR  ILKN+   +    R ++ QLL       A++RVE V++E 
Sbjct: 7   RGFKPAKCKTALQMANSRLKILKNKKEIQIKQLRRELAQLLESGQTPTARIRVEHVVREE 66

Query: 73  NMMDALGMIEDYCSLLIEKVALLQMNKECPGEVKEAISSLIFASSRFGEFPELQEIRRIF 132
             + A  +I  YC LL+ ++ +++  K CP ++KEA++S++FAS R  + PEL EI + F
Sbjct: 67  KTVAAYELIGIYCELLVVRLGVIESQKNCPIDLKEAVTSVLFASQRLSDVPELSEIFKQF 126

Query: 133 ELKFGKEFASSAVNLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIASENGITLHLEE-- 192
             K+GK+F++SAV LR + GVS  +++K S K P+  TK+K+L  IA E+ +    +   
Sbjct: 127 TTKYGKDFSTSAVELRPDSGVSRLLVEKLSAKAPDGPTKVKILMAIAEEHNVVWEAQSFV 186

Query: 193 ----------------EPAIVIKVNQKMETKK---------------------------- 252
                           +PA  + ++  + + K                            
Sbjct: 187 ESDPKDTELLNGANSFQPASSMNMDSSINSNKEQPPNIHAPATVNAHHGSSERHHSPENS 246

Query: 253 --SGDLDNPEVMNTTD------------------------NPHEVIMSEETASESVRAKK 312
             +G   +    N T                         NP+    +  + ++     +
Sbjct: 247 YANGGRSSSRSNNVTSGKADDYYHSKARPSRSRPDEGECRNPNHGYENSSSRNKQKWEPE 306

Query: 313 FKDAASAAQEAFQSAAYAALAAKAAIELS------RSESQDIHIEEKNSDL--ESSHEPK 372
           F D+  AA+ A ++A  A+ AA+AA ELS      R +S   HI   + +L  E SH   
Sbjct: 307 FVDSTDAARAAAEAAERASFAARAAAELSNKERMTRQDSTQSHISSASVNLRNEPSHRRD 366

Query: 373 INPDELESCSS-----------EYEDQDAAESNQEEIPEEPAKEIMLSDPNLDSDISNLK 375
            +  + ES S            +YED D    ++ +  E+      +  P+    + N +
Sbjct: 367 RSNAQRESFSEDHVSPRRNVRMQYEDMDRTRQDRYDRAEQIPP---VDQPSGRHSVDNSR 426

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q54I391.1e-1531.29IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1[more]
Q3ZBV12.4e-1026.74IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1[more]
P539903.1e-1026.90IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1[more]
Q9CX003.1e-1026.90IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1[more]
Q568Z63.1e-1026.90IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAG6581175.19.95e-253100.00IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022934804.11.49e-24396.89uncharacterized protein LOC111441867 [Cucurbita moschata][more]
XP_022983568.17.04e-24295.85uncharacterized protein LOC111482134 [Cucurbita maxima][more]
XP_023527290.18.20e-23892.04uncharacterized protein LOC111790567 [Cucurbita pepo subsp. pepo][more]
XP_038875575.11.26e-18775.48uncharacterized protein LOC120067980 isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1F3M17.19e-24496.89uncharacterized protein LOC111441867 OS=Cucurbita moschata OX=3662 GN=LOC1114418... [more]
A0A6J1J8383.41e-24295.85uncharacterized protein LOC111482134 OS=Cucurbita maxima OX=3661 GN=LOC111482134... [more]
A0A0A0L6Y21.58e-17170.38Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G271380 PE=3 SV=1[more]
A0A5D3CIE39.26e-16768.57Putative Regulator of Vps4 activity in the MVB pathway protein OS=Cucumis melo v... [more]
A0A1S3CEY62.16e-16568.10uncharacterized protein LOC103500154 OS=Cucumis melo OX=3656 GN=LOC103500154 PE=... [more]
Match NameE-valueIdentityDescription
AT1G13340.15.0e-4837.31Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G34220.22.3e-2936.26Regulator of Vps4 activity in the MVB pathway protein [more]
AT4G29440.13.5e-2527.64Regulator of Vps4 activity in the MVB pathway protein [more]
AT4G35730.11.0e-2428.42Regulator of Vps4 activity in the MVB pathway protein [more]
AT2G19710.16.6e-2425.39Regulator of Vps4 activity in the MVB pathway protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005061Vacuolar protein sorting-associated protein Ist1PFAMPF03398Ist1coord: 21..184
e-value: 9.1E-50
score: 168.8
IPR005061Vacuolar protein sorting-associated protein Ist1PANTHERPTHR12161IST1 FAMILY MEMBERcoord: 11..361
IPR042277Vacuolar protein sorting-associated protein IST1-likeGENE3D1.20.1260.60coord: 9..193
e-value: 2.8E-60
score: 205.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 272..299
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 300..316
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 332..353
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 272..365
NoneNo IPR availablePANTHERPTHR12161:SF65VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN IST1-RELATEDcoord: 11..361

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g151230.m01Csor.00g151230.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015031 protein transport