Csor.00g148620 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g148620
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description9-cis-epoxycarotenoid dioxygenase
LocationCsor_Chr14: 5631153 .. 5632919 (+)
RNA-Seq ExpressionCsor.00g148620
SyntenyCsor.00g148620
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSsinglestart_codonpolypeptidestop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCTTCTTCTCCTTCTCCTCCTTGTCTGGATTACTTGGGGTTTCATAATTCCACGAAATCAATTAATGGTTTCAAGAATGTTAACCTCATCAGGCAACCTTCCAATCACAACGCCGTTATTCACTCCGCTCTTCACTCCCCTTCTGTTCTTCATTTCCCCAATCATTCTCCTAATTTTCACGACAATCTCATTAAATCCCCTAATTCCAACTGGAATTTCCTTCAGAAATCCGCTTCAATCGCCCTCGACATGGCGGAATCCGCCTTGCTTTCCCACGAGCTCCGCCACCCACTCCCTAAAACCGCCGACCCACGTGTCCAAATCTCCGGCAACTTCGCTCCCGTCCCCGAACAACCCGTCAAACATAACCTCCCTGTCACCGGAACCATCCCGGATTGCATTAACGGTGTCTACCTCCGCAACGGTGCTAATCCTCTCTTTGAACCCACCGCCGGTCATCACCTATTCGACGGCGACGGTATGGTCCACGCCGTCACCATCAATGACGGCTCTGCTAGCTATGCCTGCCGGTTCACGCAGACACAACGTCTGGCTCAGGAACAACAGCTCGGGCGACCGATTTTCCCTAAAGCGATTGGCGAACTCCATGGGCACTCCGGAATTGCTCGGCTGTTACTATTCTACGCGCGAGGGATGTTCGGGCTGGTGGACCATAAGAAGGGAACCGGAGTGGCAAACGCCGGTTTGGTGTATTTTAACGACCGGCTTTTGGCGATGTCAGAAGACGATTTGCCGTACCACGTGCGTATCACGCCTTCAGGAGACCTTGAAACTGTAGGCCGTTACGATTTCAACACACAGCTTGATTCCACAATGATCGCACATCCGAAAGTGGACCCAGTCTCCAGAGAGCTCTACGCCTTAAGCTACGACGTCGTTCGGAAGCCGTATCTCAAGTACTTCCGATTCTCCCCCGACGGAATTAAATCCAAAGACGTTGAAATCCCTCTTCAAACTCCGACCATGATGCACGATTTCGCCATCACCGAGAATTTCGTTGTAATCCCCGACCAGCAAGTCGTGTTCAAGCTTCAAGAAATGCTCAAGGGCGGATCTCCGGTAATTTACGACAAGAACAAGAAATCCCGATTCGGAATTCTCCCTAAAAATGCCACAAATTCCGATGATTTAATCTGGGTGGAATCGCCGGACACCTTCTGCTTCCATTTGTGGAATGCTTGGGAGGAGCCGGAGACAGAGGAGGTCGTGGTTATTGGGTCTTGCATGACGCCGCCGGATTCAATTTTCAACGAGTGCGACGAGAATTTAAAAAGCGTACTGTCGGAAATTCGGCTGAATTTGAGAACGGGGAAATCCACGCGCCGCCCAATTATCAGCGATGAGAAGGAGCAGGTGAATCTAGAAGCCGGAATGGTGAATAGAAACCGTCTGGGGCGGAAAACCCGATATGCGTATCTTGCGATTGCAGAGCCATGGCCGAAGGTTTCGGGATTCGCGAAGGTTGATCTAGTAACAGGGGAGGTAAAAAAGCATATTTACGGCGGTAGAAAATTCGGTGGGGAGCCGTTTTTTTTACAGAAAGAAGGGAATTCAGGGGAGAAGGAAGACGAAGGGTACATTTTAGCGTTCGTCCACGATGAAAGGACATGGAAATCAGAGCTACAGATAGTGAACGCCATGGATTTGAAAGTGGAAGCTACAGTGAAGCTGCCATCCAGAGTACCCTATGGCTTCCACGGGACATTCGTCGACGCAAAACACTTGCAAAATCAAGCTTAA

mRNA sequence

ATGGCGGCTTCTTCTCCTTCTCCTCCTTGTCTGGATTACTTGGGGTTTCATAATTCCACGAAATCAATTAATGGTTTCAAGAATGTTAACCTCATCAGGCAACCTTCCAATCACAACGCCGTTATTCACTCCGCTCTTCACTCCCCTTCTGTTCTTCATTTCCCCAATCATTCTCCTAATTTTCACGACAATCTCATTAAATCCCCTAATTCCAACTGGAATTTCCTTCAGAAATCCGCTTCAATCGCCCTCGACATGGCGGAATCCGCCTTGCTTTCCCACGAGCTCCGCCACCCACTCCCTAAAACCGCCGACCCACGTGTCCAAATCTCCGGCAACTTCGCTCCCGTCCCCGAACAACCCGTCAAACATAACCTCCCTGTCACCGGAACCATCCCGGATTGCATTAACGGTGTCTACCTCCGCAACGGTGCTAATCCTCTCTTTGAACCCACCGCCGGTCATCACCTATTCGACGGCGACGGTATGGTCCACGCCGTCACCATCAATGACGGCTCTGCTAGCTATGCCTGCCGGTTCACGCAGACACAACGTCTGGCTCAGGAACAACAGCTCGGGCGACCGATTTTCCCTAAAGCGATTGGCGAACTCCATGGGCACTCCGGAATTGCTCGGCTGTTACTATTCTACGCGCGAGGGATGTTCGGGCTGGTGGACCATAAGAAGGGAACCGGAGTGGCAAACGCCGGTTTGGTGTATTTTAACGACCGGCTTTTGGCGATGTCAGAAGACGATTTGCCGTACCACGTGCGTATCACGCCTTCAGGAGACCTTGAAACTGTAGGCCGTTACGATTTCAACACACAGCTTGATTCCACAATGATCGCACATCCGAAAGTGGACCCAGTCTCCAGAGAGCTCTACGCCTTAAGCTACGACGTCGTTCGGAAGCCGTATCTCAAGTACTTCCGATTCTCCCCCGACGGAATTAAATCCAAAGACGTTGAAATCCCTCTTCAAACTCCGACCATGATGCACGATTTCGCCATCACCGAGAATTTCGTTGTAATCCCCGACCAGCAAGTCGTGTTCAAGCTTCAAGAAATGCTCAAGGGCGGATCTCCGGTAATTTACGACAAGAACAAGAAATCCCGATTCGGAATTCTCCCTAAAAATGCCACAAATTCCGATGATTTAATCTGGGTGGAATCGCCGGACACCTTCTGCTTCCATTTGTGGAATGCTTGGGAGGAGCCGGAGACAGAGGAGGTCGTGGTTATTGGGTCTTGCATGACGCCGCCGGATTCAATTTTCAACGAGTGCGACGAGAATTTAAAAAGCGTACTGTCGGAAATTCGGCTGAATTTGAGAACGGGGAAATCCACGCGCCGCCCAATTATCAGCGATGAGAAGGAGCAGGTGAATCTAGAAGCCGGAATGGTGAATAGAAACCGTCTGGGGCGGAAAACCCGATATGCGTATCTTGCGATTGCAGAGCCATGGCCGAAGGTTTCGGGATTCGCGAAGGTTGATCTAGTAACAGGGGAGGTAAAAAAGCATATTTACGGCGGTAGAAAATTCGGTGGGGAGCCGTTTTTTTTACAGAAAGAAGGGAATTCAGGGGAGAAGGAAGACGAAGGGTACATTTTAGCGTTCGTCCACGATGAAAGGACATGGAAATCAGAGCTACAGATAGTGAACGCCATGGATTTGAAAGTGGAAGCTACAGTGAAGCTGCCATCCAGAGTACCCTATGGCTTCCACGGGACATTCGTCGACGCAAAACACTTGCAAAATCAAGCTTAA

Coding sequence (CDS)

ATGGCGGCTTCTTCTCCTTCTCCTCCTTGTCTGGATTACTTGGGGTTTCATAATTCCACGAAATCAATTAATGGTTTCAAGAATGTTAACCTCATCAGGCAACCTTCCAATCACAACGCCGTTATTCACTCCGCTCTTCACTCCCCTTCTGTTCTTCATTTCCCCAATCATTCTCCTAATTTTCACGACAATCTCATTAAATCCCCTAATTCCAACTGGAATTTCCTTCAGAAATCCGCTTCAATCGCCCTCGACATGGCGGAATCCGCCTTGCTTTCCCACGAGCTCCGCCACCCACTCCCTAAAACCGCCGACCCACGTGTCCAAATCTCCGGCAACTTCGCTCCCGTCCCCGAACAACCCGTCAAACATAACCTCCCTGTCACCGGAACCATCCCGGATTGCATTAACGGTGTCTACCTCCGCAACGGTGCTAATCCTCTCTTTGAACCCACCGCCGGTCATCACCTATTCGACGGCGACGGTATGGTCCACGCCGTCACCATCAATGACGGCTCTGCTAGCTATGCCTGCCGGTTCACGCAGACACAACGTCTGGCTCAGGAACAACAGCTCGGGCGACCGATTTTCCCTAAAGCGATTGGCGAACTCCATGGGCACTCCGGAATTGCTCGGCTGTTACTATTCTACGCGCGAGGGATGTTCGGGCTGGTGGACCATAAGAAGGGAACCGGAGTGGCAAACGCCGGTTTGGTGTATTTTAACGACCGGCTTTTGGCGATGTCAGAAGACGATTTGCCGTACCACGTGCGTATCACGCCTTCAGGAGACCTTGAAACTGTAGGCCGTTACGATTTCAACACACAGCTTGATTCCACAATGATCGCACATCCGAAAGTGGACCCAGTCTCCAGAGAGCTCTACGCCTTAAGCTACGACGTCGTTCGGAAGCCGTATCTCAAGTACTTCCGATTCTCCCCCGACGGAATTAAATCCAAAGACGTTGAAATCCCTCTTCAAACTCCGACCATGATGCACGATTTCGCCATCACCGAGAATTTCGTTGTAATCCCCGACCAGCAAGTCGTGTTCAAGCTTCAAGAAATGCTCAAGGGCGGATCTCCGGTAATTTACGACAAGAACAAGAAATCCCGATTCGGAATTCTCCCTAAAAATGCCACAAATTCCGATGATTTAATCTGGGTGGAATCGCCGGACACCTTCTGCTTCCATTTGTGGAATGCTTGGGAGGAGCCGGAGACAGAGGAGGTCGTGGTTATTGGGTCTTGCATGACGCCGCCGGATTCAATTTTCAACGAGTGCGACGAGAATTTAAAAAGCGTACTGTCGGAAATTCGGCTGAATTTGAGAACGGGGAAATCCACGCGCCGCCCAATTATCAGCGATGAGAAGGAGCAGGTGAATCTAGAAGCCGGAATGGTGAATAGAAACCGTCTGGGGCGGAAAACCCGATATGCGTATCTTGCGATTGCAGAGCCATGGCCGAAGGTTTCGGGATTCGCGAAGGTTGATCTAGTAACAGGGGAGGTAAAAAAGCATATTTACGGCGGTAGAAAATTCGGTGGGGAGCCGTTTTTTTTACAGAAAGAAGGGAATTCAGGGGAGAAGGAAGACGAAGGGTACATTTTAGCGTTCGTCCACGATGAAAGGACATGGAAATCAGAGCTACAGATAGTGAACGCCATGGATTTGAAAGTGGAAGCTACAGTGAAGCTGCCATCCAGAGTACCCTATGGCTTCCACGGGACATTCGTCGACGCAAAACACTTGCAAAATCAAGCTTAA

Protein sequence

MAASSPSPPCLDYLGFHNSTKSINGFKNVNLIRQPSNHNAVIHSALHSPSVLHFPNHSPNFHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA
Homology
BLAST of Csor.00g148620 vs. ExPASy Swiss-Prot
Match: K4CJJ1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NCED2 PE=2 SV=1)

HSP 1 Score: 898.3 bits (2320), Expect = 4.7e-260
Identity = 429/547 (78.43%), Postives = 491/547 (89.76%), Query Frame = 0

Query: 42  IHSALHS-PSVLHFPNHSPNFHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPL 101
           IHSAL + P   + P + P F         S+WNF QK+A+ ALD+ ESAL+S EL++PL
Sbjct: 43  IHSALLTLPKQNNTPKNQPQFQ-------TSHWNFFQKAAAKALDIVESALVSRELQNPL 102

Query: 102 PKTADPRVQISGNFAPVPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDG 161
           PKTADPRVQI+GNFAPVPEQ V+HNLPVTGTIPDCINGVY+RNGANPLFEP AGHHLFDG
Sbjct: 103 PKTADPRVQIAGNFAPVPEQSVRHNLPVTGTIPDCINGVYVRNGANPLFEPVAGHHLFDG 162

Query: 162 DGMVHAVTINDGSASYACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARG 221
           DGMVHAVT+ +GS SY+CRFT+T+RL QE++LG P+FPKAIGELHGHSGIARLLLFYARG
Sbjct: 163 DGMVHAVTVENGSVSYSCRFTETERLVQERELGHPVFPKAIGELHGHSGIARLLLFYARG 222

Query: 222 MFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDST 281
           +FGLVDH  GTGVANAGLV+FN+RLLAMSEDD+PYHV++ PSGDL+TVGRY+F+ QL ST
Sbjct: 223 VFGLVDHSHGTGVANAGLVFFNNRLLAMSEDDVPYHVQVLPSGDLQTVGRYNFDDQLKST 282

Query: 282 MIAHPKVDPVSRELYALSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITEN 341
           MIAHPK+DPVS EL+ALSYDVV+KPYLK F+FSPDG KS DVEIPL  PTMMHDFAITEN
Sbjct: 283 MIAHPKIDPVSGELFALSYDVVQKPYLKSFKFSPDGEKSPDVEIPLDVPTMMHDFAITEN 342

Query: 342 FVVIPDQQVVFKLQEMLKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLW 401
           +VVIPDQQVVFKLQEM+KGGSPVIYDKNKKSRFGILPKNA NS+++IWVES +TFCFHLW
Sbjct: 343 YVVIPDQQVVFKLQEMIKGGSPVIYDKNKKSRFGILPKNAENSENIIWVESAETFCFHLW 402

Query: 402 NAWEEPETEEVVVIGSCMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQ 461
           NAWEEPET+EV+VIGSCMTPPDSIFNEC+ENLKSVLSEIRLNL+TG+STRR ++S   +Q
Sbjct: 403 NAWEEPETDEVIVIGSCMTPPDSIFNECNENLKSVLSEIRLNLKTGESTRRQLLS-PSDQ 462

Query: 462 VNLEAGMVNRNRLGRKTRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFF 521
           VNLEAGMVNRN+LGRKT++AYLAIAEPWPKVSGFAKVDL TGE+KKHIYG +++GGEP F
Sbjct: 463 VNLEAGMVNRNKLGRKTQFAYLAIAEPWPKVSGFAKVDLSTGEIKKHIYGDKRYGGEPLF 522

Query: 522 LQKEGNSGEKEDEGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVD 581
           L +  NS EKED+GYILAF HDE+TWKSELQIVNAM L++EATVKLPSRVPYGFHGTF+ 
Sbjct: 523 LPRNVNS-EKEDDGYILAFCHDEKTWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFIS 580

Query: 582 AKHLQNQ 588
           +K LQNQ
Sbjct: 583 SKDLQNQ 580

BLAST of Csor.00g148620 vs. ExPASy Swiss-Prot
Match: O24023 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NCED1 PE=1 SV=2)

HSP 1 Score: 883.2 bits (2281), Expect = 1.6e-255
Identity = 424/576 (73.61%), Postives = 487/576 (84.55%), Query Frame = 0

Query: 28  NVNLIRQPSNHNAV-IHSALHSPSVLHFPNHSPNF--------------HDNLIKSPNSN 87
           +++L++   N  ++ I+S+L +P +LHFP  S N+              ++N   S  S 
Sbjct: 31  SISLLKNQHNRQSLNINSSLQAPPILHFPKQSSNYQTPKNNTISHPKQENNNSSSSSTSK 90

Query: 88  WNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQPVKHNLPVTGTI 147
           WN +QK+A++ALD  ESAL  HEL HPLPKTADPRVQISGNFAPVPE PV  +LPVTG I
Sbjct: 91  WNLVQKAAAMALDAVESALTKHELEHPLPKTADPRVQISGNFAPVPENPVCQSLPVTGKI 150

Query: 148 PDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRFTQTQRLAQEQQL 207
           P C+ GVY+RNGANPLFEPTAGHH FDGDGMVHAV   +GSASYACRFT+T+RL QE+ L
Sbjct: 151 PKCVQGVYVRNGANPLFEPTAGHHFFDGDGMVHAVQFKNGSASYACRFTETERLVQEKAL 210

Query: 208 GRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVYFNDRLLAMSEDD 267
           GRP+FPKAIGELHGHSGIARL+LFYARG+FGLVDH KGTGVANAGLVYFN+RLLAMSEDD
Sbjct: 211 GRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSKGTGVANAGLVYFNNRLLAMSEDD 270

Query: 268 LPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYDVVRKPYLKYFRF 327
           LPYHV++TP+GDL+T GR+DF+ QL STMIAHPK+DPVS EL+ALSYDV++KPYLKYFRF
Sbjct: 271 LPYHVKVTPTGDLKTEGRFDFDGQLKSTMIAHPKLDPVSGELFALSYDVIQKPYLKYFRF 330

Query: 328 SPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGGSPVIYDKNKKSR 387
           S +G KS DVEIP++ PTMMHDFAITENFVVIPDQQVVFK+ EM++GGSPV+YDKNK SR
Sbjct: 331 SKNGEKSNDVEIPVEDPTMMHDFAITENFVVIPDQQVVFKMSEMIRGGSPVVYDKNKVSR 390

Query: 388 FGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECDENL 447
           FGIL K A +  DL WVE PD FCFHLWNAWEE ET+E+VVIGSCMTPPDSIFNECDE L
Sbjct: 391 FGILDKYAKDGSDLKWVEVPDCFCFHLWNAWEEAETDEIVVIGSCMTPPDSIFNECDEGL 450

Query: 448 KSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYAYLAIAEPWPKVS 507
           KSVLSEIRLNL+TGKSTR+ II +  EQVNLEAGMVNRN+LGRKT YAYLAIAEPWPKVS
Sbjct: 451 KSVLSEIRLNLKTGKSTRKSIIENPDEQVNLEAGMVNRNKLGRKTEYAYLAIAEPWPKVS 510

Query: 508 GFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFVHDERTWKSELQI 567
           GFAKV+L TGEV+K IYG  K+GGEP FL ++ NS E ED+GYILAFVHDE+ WKSELQI
Sbjct: 511 GFAKVNLFTGEVEKFIYGDNKYGGEPLFLPRDPNSKE-EDDGYILAFVHDEKEWKSELQI 570

Query: 568 VNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA 589
           VNAM LK+EATVKLPSRVPYGFHGTF++A  L NQA
Sbjct: 571 VNAMSLKLEATVKLPSRVPYGFHGTFINANDLANQA 605

BLAST of Csor.00g148620 vs. ExPASy Swiss-Prot
Match: Q9LRR7 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED3 PE=1 SV=1)

HSP 1 Score: 847.0 bits (2187), Expect = 1.2e-244
Identity = 409/551 (74.23%), Postives = 460/551 (83.48%), Query Frame = 0

Query: 42  IHSALHSPSVLHFPNHSPNFHDNLIK-----SPNSNWNFLQKSASIALDMAESALLSHEL 101
           + SALH+P  LHFP  S N    ++K     S     N  Q++A+ ALD AE  L+SHE 
Sbjct: 50  VSSALHTPPALHFPKQSSNSPAIVVKPKAKESNTKQMNLFQRAAAAALDAAEGFLVSHEK 109

Query: 102 RHPLPKTADPRVQISGNFAPVPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHH 161
            HPLPKTADP VQI+GNFAPV EQPV+ NLPV G +PD I GVY+RNGANPL EP  GHH
Sbjct: 110 LHPLPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHH 169

Query: 162 LFDGDGMVHAVTINDGSASYACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLF 221
            FDGDGMVHAV    GSASYACRFTQT R  QE+QLGRP+FPKAIGELHGH+GIARL+LF
Sbjct: 170 FFDGDGMVHAVKFEHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLMLF 229

Query: 222 YARGMFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQ 281
           YAR   G+VD   GTGVANAGLVYFN RLLAMSEDDLPY V+ITP+GDL+TVGR+DF+ Q
Sbjct: 230 YARAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQ 289

Query: 282 LDSTMIAHPKVDPVSRELYALSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFA 341
           L+STMIAHPKVDP S EL+ALSYDVV KPYLKYFRFSPDG KS DVEI L  PTMMHDFA
Sbjct: 290 LESTMIAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDFA 349

Query: 342 ITENFVVIPDQQVVFKLQEMLKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFC 401
           ITENFVV+PDQQVVFKL EM++GGSPV+YDKNK +RFGIL K A +S ++ W+++PD FC
Sbjct: 350 ITENFVVVPDQQVVFKLPEMIRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFC 409

Query: 402 FHLWNAWEEPETEEVVVIGSCMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISD 461
           FHLWNAWEEPET+EVVVIGSCMTPPDSIFNE DENLKSVLSEIRLNL+TG+STRRPIIS+
Sbjct: 410 FHLWNAWEEPETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIISN 469

Query: 462 EKEQVNLEAGMVNRNRLGRKTRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGG 521
           E +QVNLEAGMVNRN LGRKT++AYLA+AEPWPKVSGFAKVDL TGEVKKH+YG  ++GG
Sbjct: 470 EDQQVNLEAGMVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGEVKKHLYGDNRYGG 529

Query: 522 EPFFLQKEGNSGEKEDEGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHG 581
           EP FL  EG    +EDEGYIL FVHDE+TWKSELQIVNA+ L+VEATVKLPSRVPYGFHG
Sbjct: 530 EPLFLPGEGG---EEDEGYILCFVHDEKTWKSELQIVNAVSLEVEATVKLPSRVPYGFHG 589

Query: 582 TFVDAKHLQNQ 588
           TF+ A  L  Q
Sbjct: 590 TFIGADDLAKQ 597

BLAST of Csor.00g148620 vs. ExPASy Swiss-Prot
Match: Q9M6E8 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris OX=3885 GN=NCED1 PE=2 SV=1)

HSP 1 Score: 843.2 bits (2177), Expect = 1.8e-243
Identity = 398/516 (77.13%), Postives = 457/516 (88.57%), Query Frame = 0

Query: 73  WNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQPVKHNLPVTGTI 132
           WNFLQK+A+  LDM E+AL+SHE +HPLPKTADP+VQI+GNFAPVPE      LPV G I
Sbjct: 105 WNFLQKAAATGLDMVETALVSHESKHPLPKTADPKVQIAGNFAPVPEHAADQALPVVGKI 164

Query: 133 PDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRFTQTQRLAQEQQL 192
           P CI+GVY+RNGANPL+EP AGHH FDGDGMVHAV   +G+ASYACRFT+TQRLAQE+ L
Sbjct: 165 PKCIDGVYVRNGANPLYEPVAGHHFFDGDGMVHAVKFTNGAASYACRFTETQRLAQEKSL 224

Query: 193 GRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVYFNDRLLAMSEDD 252
           GRP+FPKAIGELHGHSGIARLLLFYAR +F LVD   G GVANAGLVYFN+ LLAMSEDD
Sbjct: 225 GRPVFPKAIGELHGHSGIARLLLFYARSLFQLVDGSHGMGVANAGLVYFNNHLLAMSEDD 284

Query: 253 LPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYDVVRKPYLKYFRF 312
           LPYHVRIT +GDL TVGRYDFN QL+STMIAHPK+DPV+ +L+ALSYDVV+KPYLKYFRF
Sbjct: 285 LPYHVRITSNGDLTTVGRYDFNGQLNSTMIAHPKLDPVNGDLHALSYDVVQKPYLKYFRF 344

Query: 313 SPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGGSPVIYDKNKKSR 372
           S DG+KS DVEIPL+ PTMMHDFAITENFVV+PDQQVVFKL EM+ GGSPV+YDKNK SR
Sbjct: 345 SADGVKSPDVEIPLKEPTMMHDFAITENFVVVPDQQVVFKLTEMITGGSPVVYDKNKTSR 404

Query: 373 FGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECDENL 432
           FGIL KNA +++ + W+++P+ FCFHLWNAWEEPET+E+VVIGSCMTP DSIFNECDE+L
Sbjct: 405 FGILDKNAKDANAMRWIDAPECFCFHLWNAWEEPETDEIVVIGSCMTPADSIFNECDESL 464

Query: 433 KSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYAYLAIAEPWPKVS 492
           KSVLSEIRLNLRTGKSTRRPIISD  EQVNLEAGMVNRN+LGRKT++AYLA+AEPWPKVS
Sbjct: 465 KSVLSEIRLNLRTGKSTRRPIISD-AEQVNLEAGMVNRNKLGRKTQFAYLALAEPWPKVS 524

Query: 493 GFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFVHDERTWKSELQI 552
           GFAKVDL +GEV+K++YG  KFGGEP FL     +GE+E +GYILAFVHDE+ WKSELQI
Sbjct: 525 GFAKVDLFSGEVQKYMYGEEKFGGEPLFLP----NGEEEGDGYILAFVHDEKEWKSELQI 584

Query: 553 VNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA 589
           VNA +LK+EA++KLPSRVPYGFHGTF+ +K L+ QA
Sbjct: 585 VNAQNLKLEASIKLPSRVPYGFHGTFIHSKDLRKQA 615

BLAST of Csor.00g148620 vs. ExPASy Swiss-Prot
Match: Q9C6Z1 (Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED5 PE=1 SV=1)

HSP 1 Score: 837.0 bits (2161), Expect = 1.3e-241
Identity = 403/555 (72.61%), Postives = 470/555 (84.68%), Query Frame = 0

Query: 34  QPSNHNAVIHSALHSPSVLHFPNHSPNFHDNLIKSPNSNWNFLQKSASIALDMAESALLS 93
           +P       +S   +P++L+FPN+ P+ +  + +   S WN LQ++AS ALD AE+ALL 
Sbjct: 38  KPRRRKLSANSVSDTPNLLNFPNY-PSPNPIIPEKDTSRWNPLQRAASAALDFAETALLR 97

Query: 94  HELRHPLPKTADPRVQISGNFAPVPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFEPTA 153
            E   PLPKT DPR QISGN+APVPEQ VK +L V G IPDCI+GVYLRNGANPLFEP +
Sbjct: 98  RERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLSVDGKIPDCIDGVYLRNGANPLFEPVS 157

Query: 154 GHHLFDGDGMVHAVTINDGSASYACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARL 213
           GHHLFDGDGMVHAV I +G ASY+CRFT+T+RL QE+QLG PIFPKAIGELHGHSGIARL
Sbjct: 158 GHHLFDGDGMVHAVKITNGDASYSCRFTETERLVQEKQLGSPIFPKAIGELHGHSGIARL 217

Query: 214 LLFYARGMFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDF 273
           +LFYARG+FGL++HK GTGVANAGLVYF+DRLLAMSEDDLPY VR+T +GDLET+GR+DF
Sbjct: 218 MLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAMSEDDLPYQVRVTDNGDLETIGRFDF 277

Query: 274 NTQLDSTMIAHPKVDPVSRELYALSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMH 333
           + QL S MIAHPK+DPV++EL+ALSYDVV+KPYLKYF+FSP+G KS DVEIPL +PTMMH
Sbjct: 278 DGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYLKYFKFSPEGEKSPDVEIPLASPTMMH 337

Query: 334 DFAITENFVVIPDQQVVFKLQEMLKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPD 393
           DFAITENFVVIPDQQVVFKL +M  G SPV YD  K SRFGILP+NA ++ +++WVESP+
Sbjct: 338 DFAITENFVVIPDQQVVFKLSDMFLGKSPVKYDGEKISRFGILPRNAKDASEMVWVESPE 397

Query: 394 TFCFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPI 453
           TFCFHLWNAWE PET+EVVVIGSCMTP DSIFNECDE L SVLSEIRLNL+TGKSTRR I
Sbjct: 398 TFCFHLWNAWESPETDEVVVIGSCMTPADSIFNECDEQLNSVLSEIRLNLKTGKSTRRTI 457

Query: 454 ISDEKEQVNLEAGMVNRNRLGRKTRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRK 513
           I     Q+NLEAGMVNRN LGRKTRYAYLAIAEPWPKVSGFAKVDL TGEVK H YGG+K
Sbjct: 458 IPG-SVQMNLEAGMVNRNLLGRKTRYAYLAIAEPWPKVSGFAKVDLSTGEVKNHFYGGKK 517

Query: 514 FGGEPFFLQKEGNSGEKEDEGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYG 573
           +GGEPFFL + G   + ED+GYI++FVHDE +W+SEL IVNA+ L++EATVKLPSRVPYG
Sbjct: 518 YGGEPFFLPR-GLESDGEDDGYIMSFVHDEESWESELHIVNAVTLELEATVKLPSRVPYG 577

Query: 574 FHGTFVDAKHLQNQA 589
           FHGTFV++  + NQA
Sbjct: 578 FHGTFVNSADMLNQA 589

BLAST of Csor.00g148620 vs. NCBI nr
Match: KAG6581433.1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034725.1 9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1201 bits (3106), Expect = 0.0
Identity = 588/588 (100.00%), Postives = 588/588 (100.00%), Query Frame = 0

Query: 1   MAASSPSPPCLDYLGFHNSTKSINGFKNVNLIRQPSNHNAVIHSALHSPSVLHFPNHSPN 60
           MAASSPSPPCLDYLGFHNSTKSINGFKNVNLIRQPSNHNAVIHSALHSPSVLHFPNHSPN
Sbjct: 1   MAASSPSPPCLDYLGFHNSTKSINGFKNVNLIRQPSNHNAVIHSALHSPSVLHFPNHSPN 60

Query: 61  FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ 120
           FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ
Sbjct: 61  FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ 120

Query: 121 PVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF 180
           PVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF
Sbjct: 121 PVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF 180

Query: 181 TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY 240
           TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY
Sbjct: 181 TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY 240

Query: 241 FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD 300
           FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD
Sbjct: 241 FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD 300

Query: 301 VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG 360
           VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG
Sbjct: 301 VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG 360

Query: 361 SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP 420
           SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP
Sbjct: 361 SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP 420

Query: 421 PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA 480
           PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA
Sbjct: 421 PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA 480

Query: 481 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV 540
           YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV
Sbjct: 481 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV 540

Query: 541 HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA 588
           HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA
Sbjct: 541 HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA 588

BLAST of Csor.00g148620 vs. NCBI nr
Match: XP_022926148.1 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1198 bits (3099), Expect = 0.0
Identity = 587/588 (99.83%), Postives = 587/588 (99.83%), Query Frame = 0

Query: 1   MAASSPSPPCLDYLGFHNSTKSINGFKNVNLIRQPSNHNAVIHSALHSPSVLHFPNHSPN 60
           MAASSPSPPCLDYLGFHNSTKSINGFKNVN IRQPSNHNAVIHSALHSPSVLHFPNHSPN
Sbjct: 1   MAASSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNAVIHSALHSPSVLHFPNHSPN 60

Query: 61  FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ 120
           FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ
Sbjct: 61  FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ 120

Query: 121 PVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF 180
           PVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF
Sbjct: 121 PVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF 180

Query: 181 TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY 240
           TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY
Sbjct: 181 TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY 240

Query: 241 FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD 300
           FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD
Sbjct: 241 FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD 300

Query: 301 VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG 360
           VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG
Sbjct: 301 VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG 360

Query: 361 SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP 420
           SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP
Sbjct: 361 SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP 420

Query: 421 PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA 480
           PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA
Sbjct: 421 PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA 480

Query: 481 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV 540
           YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV
Sbjct: 481 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV 540

Query: 541 HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA 588
           HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA
Sbjct: 541 HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA 588

BLAST of Csor.00g148620 vs. NCBI nr
Match: XP_022978939.1 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1195 bits (3092), Expect = 0.0
Identity = 585/588 (99.49%), Postives = 587/588 (99.83%), Query Frame = 0

Query: 1   MAASSPSPPCLDYLGFHNSTKSINGFKNVNLIRQPSNHNAVIHSALHSPSVLHFPNHSPN 60
           MAASSPSPPCLDYLGFHNSTKSINGFKNVN IRQPSNHNAVIHSALHSPSVLHFPNHSPN
Sbjct: 1   MAASSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNAVIHSALHSPSVLHFPNHSPN 60

Query: 61  FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ 120
           FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ
Sbjct: 61  FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ 120

Query: 121 PVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF 180
           PVK+NLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF
Sbjct: 121 PVKYNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF 180

Query: 181 TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY 240
           TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY
Sbjct: 181 TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY 240

Query: 241 FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD 300
           FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD
Sbjct: 241 FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD 300

Query: 301 VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG 360
           VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG
Sbjct: 301 VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG 360

Query: 361 SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP 420
           SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP
Sbjct: 361 SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP 420

Query: 421 PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA 480
           PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA
Sbjct: 421 PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA 480

Query: 481 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV 540
           YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV
Sbjct: 481 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV 540

Query: 541 HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA 588
           HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTF+DAKHLQNQA
Sbjct: 541 HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFIDAKHLQNQA 588

BLAST of Csor.00g148620 vs. NCBI nr
Match: XP_023543156.1 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucurbita pepo subsp. pepo] >AWW05326.1 9-cis-epoxycarotenoid dioxygenase [Cucurbita pepo])

HSP 1 Score: 1192 bits (3085), Expect = 0.0
Identity = 584/588 (99.32%), Postives = 586/588 (99.66%), Query Frame = 0

Query: 1   MAASSPSPPCLDYLGFHNSTKSINGFKNVNLIRQPSNHNAVIHSALHSPSVLHFPNHSPN 60
           MAASSPSPPCLDYLGFHNSTKSINGFKNVN IRQPSNHNA+IHSALHSPSVLHFPNHSPN
Sbjct: 1   MAASSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNALIHSALHSPSVLHFPNHSPN 60

Query: 61  FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ 120
           FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ
Sbjct: 61  FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ 120

Query: 121 PVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF 180
           PVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF
Sbjct: 121 PVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF 180

Query: 181 TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY 240
           TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY
Sbjct: 181 TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY 240

Query: 241 FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD 300
           FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSR LYALSYD
Sbjct: 241 FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRGLYALSYD 300

Query: 301 VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG 360
           VVRKPYLKYFRFSPDGIKS+DVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG
Sbjct: 301 VVRKPYLKYFRFSPDGIKSQDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG 360

Query: 361 SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP 420
           SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP
Sbjct: 361 SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP 420

Query: 421 PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA 480
           PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA
Sbjct: 421 PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA 480

Query: 481 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV 540
           YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV
Sbjct: 481 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV 540

Query: 541 HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA 588
           HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA
Sbjct: 541 HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA 588

BLAST of Csor.00g148620 vs. NCBI nr
Match: XP_038883946.1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 1051 bits (2718), Expect = 0.0
Identity = 523/600 (87.17%), Postives = 552/600 (92.00%), Query Frame = 0

Query: 1   MAASSP--SPPCLDYLGFHNSTKS----INGFKNVNLIRQPSNHNAVIHSALHSPSVLHF 60
           MAAS P  S   LD+L F NST+S     N   NV  I Q S HN +IHSALHSPSVLHF
Sbjct: 2   MAASPPPSSSSLLDHLSFSNSTRSNFPAFNYNNNVKFINQNSTHNPIIHSALHSPSVLHF 61

Query: 61  PNHSPNFHDNLI------KSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRV 120
           PN +PNFH+NL       KSP SNWN LQK+AS+AL M ESAL+SHEL+HPLPKTADPRV
Sbjct: 62  PNQTPNFHNNLTLKDQTNKSPFSNWNLLQKTASMALYMVESALVSHELQHPLPKTADPRV 121

Query: 121 QISGNFAPVPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVT 180
           QISGNFAPVPEQPVKHNLPVTGTIPDCI+GVYLRNGANPLF+PTAGHHLFDGDGMVHAVT
Sbjct: 122 QISGNFAPVPEQPVKHNLPVTGTIPDCIHGVYLRNGANPLFQPTAGHHLFDGDGMVHAVT 181

Query: 181 INDGSASYACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHK 240
           IN GSASYACRFTQTQRL QE++LGRP+FPKAIGELHGHSGIARLLLFYARG+FGLVDHK
Sbjct: 182 INGGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHK 241

Query: 241 KGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVD 300
           KGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDL+TVGRYDFN QL STMIAHPKVD
Sbjct: 242 KGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLHSTMIAHPKVD 301

Query: 301 PVSRELYALSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQ 360
           P+S+ELYALSYDVVRKPYLKYFRFSPDG+KSKDVEIPL+TPTMMHDFAITENFVVIPDQQ
Sbjct: 302 PISKELYALSYDVVRKPYLKYFRFSPDGMKSKDVEIPLETPTMMHDFAITENFVVIPDQQ 361

Query: 361 VVFKLQEMLKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPET 420
           VVFKLQEM+KGGSPVIYDK KKSRFGILPKNAT+S DLIWVESPDTFCFHLWNAWEEPET
Sbjct: 362 VVFKLQEMVKGGSPVIYDKKKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPET 421

Query: 421 EEVVVIGSCMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMV 480
           EEVVVIGSCMTPPDSIFNEC+ENLKSVLSEIRLNLRTGKSTRR II +EKEQ+NLEAGMV
Sbjct: 422 EEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQMNLEAGMV 481

Query: 481 NRNRLGRKTRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSG 540
           NRNRLGRKTR+AYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFL KEGNS 
Sbjct: 482 NRNRLGRKTRFAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNSK 541

Query: 541 EKEDEGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA 588
           E EDEGYILAFVHDERTWKSE+QIVNAMDLKVEAT+KLPSRVPYGFHGTFV +  LQNQA
Sbjct: 542 E-EDEGYILAFVHDERTWKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVQSNSLQNQA 600

BLAST of Csor.00g148620 vs. ExPASy TrEMBL
Match: A0A6J1EH85 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111433338 PE=3 SV=1)

HSP 1 Score: 1198 bits (3099), Expect = 0.0
Identity = 587/588 (99.83%), Postives = 587/588 (99.83%), Query Frame = 0

Query: 1   MAASSPSPPCLDYLGFHNSTKSINGFKNVNLIRQPSNHNAVIHSALHSPSVLHFPNHSPN 60
           MAASSPSPPCLDYLGFHNSTKSINGFKNVN IRQPSNHNAVIHSALHSPSVLHFPNHSPN
Sbjct: 1   MAASSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNAVIHSALHSPSVLHFPNHSPN 60

Query: 61  FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ 120
           FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ
Sbjct: 61  FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ 120

Query: 121 PVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF 180
           PVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF
Sbjct: 121 PVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF 180

Query: 181 TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY 240
           TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY
Sbjct: 181 TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY 240

Query: 241 FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD 300
           FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD
Sbjct: 241 FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD 300

Query: 301 VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG 360
           VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG
Sbjct: 301 VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG 360

Query: 361 SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP 420
           SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP
Sbjct: 361 SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP 420

Query: 421 PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA 480
           PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA
Sbjct: 421 PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA 480

Query: 481 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV 540
           YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV
Sbjct: 481 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV 540

Query: 541 HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA 588
           HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA
Sbjct: 541 HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA 588

BLAST of Csor.00g148620 vs. ExPASy TrEMBL
Match: A0A6J1IVE0 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111478741 PE=3 SV=1)

HSP 1 Score: 1195 bits (3092), Expect = 0.0
Identity = 585/588 (99.49%), Postives = 587/588 (99.83%), Query Frame = 0

Query: 1   MAASSPSPPCLDYLGFHNSTKSINGFKNVNLIRQPSNHNAVIHSALHSPSVLHFPNHSPN 60
           MAASSPSPPCLDYLGFHNSTKSINGFKNVN IRQPSNHNAVIHSALHSPSVLHFPNHSPN
Sbjct: 1   MAASSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNAVIHSALHSPSVLHFPNHSPN 60

Query: 61  FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ 120
           FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ
Sbjct: 61  FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ 120

Query: 121 PVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF 180
           PVK+NLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF
Sbjct: 121 PVKYNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF 180

Query: 181 TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY 240
           TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY
Sbjct: 181 TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY 240

Query: 241 FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD 300
           FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD
Sbjct: 241 FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD 300

Query: 301 VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG 360
           VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG
Sbjct: 301 VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG 360

Query: 361 SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP 420
           SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP
Sbjct: 361 SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP 420

Query: 421 PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA 480
           PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA
Sbjct: 421 PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA 480

Query: 481 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV 540
           YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV
Sbjct: 481 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV 540

Query: 541 HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA 588
           HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTF+DAKHLQNQA
Sbjct: 541 HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFIDAKHLQNQA 588

BLAST of Csor.00g148620 vs. ExPASy TrEMBL
Match: A0A2Z4GT57 (9-cis-epoxycarotenoid dioxygenase OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 1192 bits (3085), Expect = 0.0
Identity = 584/588 (99.32%), Postives = 586/588 (99.66%), Query Frame = 0

Query: 1   MAASSPSPPCLDYLGFHNSTKSINGFKNVNLIRQPSNHNAVIHSALHSPSVLHFPNHSPN 60
           MAASSPSPPCLDYLGFHNSTKSINGFKNVN IRQPSNHNA+IHSALHSPSVLHFPNHSPN
Sbjct: 1   MAASSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNALIHSALHSPSVLHFPNHSPN 60

Query: 61  FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ 120
           FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ
Sbjct: 61  FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ 120

Query: 121 PVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF 180
           PVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF
Sbjct: 121 PVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF 180

Query: 181 TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY 240
           TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY
Sbjct: 181 TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY 240

Query: 241 FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD 300
           FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSR LYALSYD
Sbjct: 241 FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRGLYALSYD 300

Query: 301 VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG 360
           VVRKPYLKYFRFSPDGIKS+DVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG
Sbjct: 301 VVRKPYLKYFRFSPDGIKSQDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG 360

Query: 361 SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP 420
           SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP
Sbjct: 361 SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP 420

Query: 421 PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA 480
           PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA
Sbjct: 421 PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA 480

Query: 481 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV 540
           YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV
Sbjct: 481 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV 540

Query: 541 HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA 588
           HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA
Sbjct: 541 HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA 588

BLAST of Csor.00g148620 vs. ExPASy TrEMBL
Match: A0A1L5JHV5 (9-cis-epoxycarotenoid dioxygenase NCED2 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 1038 bits (2683), Expect = 0.0
Identity = 519/601 (86.36%), Postives = 548/601 (91.18%), Query Frame = 0

Query: 2   AASSPSPPCLDYLGFHNSTKSINGFKNVNLIRQPSNHNAV--IHSALHSPSVLHFPNHSP 61
           A SS S   LD   F NSTKSI  FKNV + +Q +N NAV  +H ALHSPSVLHFPN + 
Sbjct: 4   APSSSSSSLLDLGAFSNSTKSIR-FKNVKISQQSNNRNAVLVVHCALHSPSVLHFPNQT- 63

Query: 62  NFHDNLI----------KSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQ 121
           N+H  L+          K PNSNWNFLQK+AS+A D+ ESALLSHEL+HPLPKTADPRVQ
Sbjct: 64  NYHKKLVLKEDTNTNKPKPPNSNWNFLQKTASMAFDIVESALLSHELQHPLPKTADPRVQ 123

Query: 122 ISGNFAPVPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTI 181
           ISGNFAPVPEQPVKHNLP+TGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAV+I
Sbjct: 124 ISGNFAPVPEQPVKHNLPITGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVSI 183

Query: 182 NDGSASYACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKK 241
           N  +ASYACRFT+TQRL QE++LGR +FPKAIGELHGHSGIARLLLFY RG+FGLVDHK 
Sbjct: 184 NGATASYACRFTETQRLVQERRLGRSVFPKAIGELHGHSGIARLLLFYGRGLFGLVDHKN 243

Query: 242 GTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDP 301
           GTGVANAGLVYFNDRLLAMSEDDLPYHVRIT SGDLETVGRYDF  QL STMIAHPKVDP
Sbjct: 244 GTGVANAGLVYFNDRLLAMSEDDLPYHVRITASGDLETVGRYDFKMQLKSTMIAHPKVDP 303

Query: 302 VSRELYALSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQV 361
           VSRELYALSYDVVRKPYLKYFRFSPDG KSKD+EIPL TPTMMHDFAITENFVVIPDQQV
Sbjct: 304 VSRELYALSYDVVRKPYLKYFRFSPDGTKSKDLEIPLHTPTMMHDFAITENFVVIPDQQV 363

Query: 362 VFKLQEMLKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETE 421
           VFKLQEM+KGGSPVIYDKNKKSRFGILPKNA++S+DLIWVESPDTFCFHLWNAWEEPET+
Sbjct: 364 VFKLQEMVKGGSPVIYDKNKKSRFGILPKNASDSNDLIWVESPDTFCFHLWNAWEEPETD 423

Query: 422 EVVVIGSCMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVN 481
           EVVVIGSCMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDE++QVNLEAGMVN
Sbjct: 424 EVVVIGSCMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEEQQVNLEAGMVN 483

Query: 482 RNRLGRKTRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSG- 541
           RNRLGRKTRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFL KEG S  
Sbjct: 484 RNRLGRKTRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGKSEK 543

Query: 542 -EKEDEGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQ 588
            E+EDEGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDA +LQNQ
Sbjct: 544 EEEEDEGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDANNLQNQ 602

BLAST of Csor.00g148620 vs. ExPASy TrEMBL
Match: A0A1S3AZU0 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103484361 PE=3 SV=1)

HSP 1 Score: 1030 bits (2663), Expect = 0.0
Identity = 511/597 (85.59%), Postives = 549/597 (91.96%), Query Frame = 0

Query: 5   SPSPPCLDYLGFHNSTKSINGFKNVNLIRQPSNHNAVIHSALHSPSVLHFPNHS------ 64
           +P  P LD+LGF NSTKS     N+N ++  +  N++IHSALHSPSVL FPN +      
Sbjct: 3   APPSPLLDHLGFLNSTKSNFPNLNINHLKSITTQNSIIHSALHSPSVLRFPNQTTTTPPP 62

Query: 65  PNFHDNLI----KSPNS-NWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGN 124
           PNFH +L     KSP + NWN LQK+ASIALDM ESAL+SHEL+HPLPKTADPRVQISGN
Sbjct: 63  PNFHKSLSLKHQKSPTAPNWNLLQKTASIALDMVESALVSHELQHPLPKTADPRVQISGN 122

Query: 125 FAPVPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGS 184
           FAPVPEQPVKHNLPVTGTIPDCINGVYLRNGANPLF+PTAGHHLFDGDGMVHAVTIN GS
Sbjct: 123 FAPVPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHAVTINHGS 182

Query: 185 ASYACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGV 244
           ASYACRFTQTQRL QE++LGRPIFPKAIGELHGHSGIARLLLFYARG+FGLVDHKKGTGV
Sbjct: 183 ASYACRFTQTQRLVQERRLGRPIFPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTGV 242

Query: 245 ANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRE 304
           ANAGLVYFNDRLLAMSEDDLPYHVRITPSGDL+TVGRYDF+ QL STMIAHPKVDP+S+E
Sbjct: 243 ANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPKVDPISKE 302

Query: 305 LYALSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKL 364
           LYALSYDVVRKPYLKYFRFSP+GIKSKDVEIP++TPTMMHDFAITENFVVIPDQQVVFKL
Sbjct: 303 LYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPVETPTMMHDFAITENFVVIPDQQVVFKL 362

Query: 365 QEMLKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVV 424
           QEM+KGGSPVIYDKNKKSRFGILPKNAT+S DLIWVESPDTFCFHLWNAWEEPETEEVVV
Sbjct: 363 QEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETEEVVV 422

Query: 425 IGSCMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRL 484
           IGSCMTPPDSIFNEC+ENLKSVLSEIRLNLRTGKSTRR II +EKEQVNLEAGMVN+ R+
Sbjct: 423 IGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGMVNKTRV 482

Query: 485 GRKTRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSG--EKE 544
           GRK+RYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFL K+ NS   E+E
Sbjct: 483 GRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKDENSKAEEEE 542

Query: 545 DEGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA 588
           DEGYILAFVHDERTWKSE+QIVNA DLKVEAT+KLPSRVPYGFHGTFV + +LQNQA
Sbjct: 543 DEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNNLQNQA 599

BLAST of Csor.00g148620 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 847.0 bits (2187), Expect = 8.8e-246
Identity = 409/551 (74.23%), Postives = 460/551 (83.48%), Query Frame = 0

Query: 42  IHSALHSPSVLHFPNHSPNFHDNLIK-----SPNSNWNFLQKSASIALDMAESALLSHEL 101
           + SALH+P  LHFP  S N    ++K     S     N  Q++A+ ALD AE  L+SHE 
Sbjct: 50  VSSALHTPPALHFPKQSSNSPAIVVKPKAKESNTKQMNLFQRAAAAALDAAEGFLVSHEK 109

Query: 102 RHPLPKTADPRVQISGNFAPVPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHH 161
            HPLPKTADP VQI+GNFAPV EQPV+ NLPV G +PD I GVY+RNGANPL EP  GHH
Sbjct: 110 LHPLPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHH 169

Query: 162 LFDGDGMVHAVTINDGSASYACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLF 221
            FDGDGMVHAV    GSASYACRFTQT R  QE+QLGRP+FPKAIGELHGH+GIARL+LF
Sbjct: 170 FFDGDGMVHAVKFEHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLMLF 229

Query: 222 YARGMFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQ 281
           YAR   G+VD   GTGVANAGLVYFN RLLAMSEDDLPY V+ITP+GDL+TVGR+DF+ Q
Sbjct: 230 YARAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQ 289

Query: 282 LDSTMIAHPKVDPVSRELYALSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFA 341
           L+STMIAHPKVDP S EL+ALSYDVV KPYLKYFRFSPDG KS DVEI L  PTMMHDFA
Sbjct: 290 LESTMIAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDFA 349

Query: 342 ITENFVVIPDQQVVFKLQEMLKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFC 401
           ITENFVV+PDQQVVFKL EM++GGSPV+YDKNK +RFGIL K A +S ++ W+++PD FC
Sbjct: 350 ITENFVVVPDQQVVFKLPEMIRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFC 409

Query: 402 FHLWNAWEEPETEEVVVIGSCMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISD 461
           FHLWNAWEEPET+EVVVIGSCMTPPDSIFNE DENLKSVLSEIRLNL+TG+STRRPIIS+
Sbjct: 410 FHLWNAWEEPETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIISN 469

Query: 462 EKEQVNLEAGMVNRNRLGRKTRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGG 521
           E +QVNLEAGMVNRN LGRKT++AYLA+AEPWPKVSGFAKVDL TGEVKKH+YG  ++GG
Sbjct: 470 EDQQVNLEAGMVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGEVKKHLYGDNRYGG 529

Query: 522 EPFFLQKEGNSGEKEDEGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHG 581
           EP FL  EG    +EDEGYIL FVHDE+TWKSELQIVNA+ L+VEATVKLPSRVPYGFHG
Sbjct: 530 EPLFLPGEGG---EEDEGYILCFVHDEKTWKSELQIVNAVSLEVEATVKLPSRVPYGFHG 589

Query: 582 TFVDAKHLQNQ 588
           TF+ A  L  Q
Sbjct: 590 TFIGADDLAKQ 597

BLAST of Csor.00g148620 vs. TAIR 10
Match: AT1G30100.1 (nine-cis-epoxycarotenoid dioxygenase 5 )

HSP 1 Score: 837.0 bits (2161), Expect = 9.1e-243
Identity = 403/555 (72.61%), Postives = 470/555 (84.68%), Query Frame = 0

Query: 34  QPSNHNAVIHSALHSPSVLHFPNHSPNFHDNLIKSPNSNWNFLQKSASIALDMAESALLS 93
           +P       +S   +P++L+FPN+ P+ +  + +   S WN LQ++AS ALD AE+ALL 
Sbjct: 38  KPRRRKLSANSVSDTPNLLNFPNY-PSPNPIIPEKDTSRWNPLQRAASAALDFAETALLR 97

Query: 94  HELRHPLPKTADPRVQISGNFAPVPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFEPTA 153
            E   PLPKT DPR QISGN+APVPEQ VK +L V G IPDCI+GVYLRNGANPLFEP +
Sbjct: 98  RERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLSVDGKIPDCIDGVYLRNGANPLFEPVS 157

Query: 154 GHHLFDGDGMVHAVTINDGSASYACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARL 213
           GHHLFDGDGMVHAV I +G ASY+CRFT+T+RL QE+QLG PIFPKAIGELHGHSGIARL
Sbjct: 158 GHHLFDGDGMVHAVKITNGDASYSCRFTETERLVQEKQLGSPIFPKAIGELHGHSGIARL 217

Query: 214 LLFYARGMFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDF 273
           +LFYARG+FGL++HK GTGVANAGLVYF+DRLLAMSEDDLPY VR+T +GDLET+GR+DF
Sbjct: 218 MLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAMSEDDLPYQVRVTDNGDLETIGRFDF 277

Query: 274 NTQLDSTMIAHPKVDPVSRELYALSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMH 333
           + QL S MIAHPK+DPV++EL+ALSYDVV+KPYLKYF+FSP+G KS DVEIPL +PTMMH
Sbjct: 278 DGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYLKYFKFSPEGEKSPDVEIPLASPTMMH 337

Query: 334 DFAITENFVVIPDQQVVFKLQEMLKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPD 393
           DFAITENFVVIPDQQVVFKL +M  G SPV YD  K SRFGILP+NA ++ +++WVESP+
Sbjct: 338 DFAITENFVVIPDQQVVFKLSDMFLGKSPVKYDGEKISRFGILPRNAKDASEMVWVESPE 397

Query: 394 TFCFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPI 453
           TFCFHLWNAWE PET+EVVVIGSCMTP DSIFNECDE L SVLSEIRLNL+TGKSTRR I
Sbjct: 398 TFCFHLWNAWESPETDEVVVIGSCMTPADSIFNECDEQLNSVLSEIRLNLKTGKSTRRTI 457

Query: 454 ISDEKEQVNLEAGMVNRNRLGRKTRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRK 513
           I     Q+NLEAGMVNRN LGRKTRYAYLAIAEPWPKVSGFAKVDL TGEVK H YGG+K
Sbjct: 458 IPG-SVQMNLEAGMVNRNLLGRKTRYAYLAIAEPWPKVSGFAKVDLSTGEVKNHFYGGKK 517

Query: 514 FGGEPFFLQKEGNSGEKEDEGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYG 573
           +GGEPFFL + G   + ED+GYI++FVHDE +W+SEL IVNA+ L++EATVKLPSRVPYG
Sbjct: 518 YGGEPFFLPR-GLESDGEDDGYIMSFVHDEESWESELHIVNAVTLELEATVKLPSRVPYG 577

Query: 574 FHGTFVDAKHLQNQA 589
           FHGTFV++  + NQA
Sbjct: 578 FHGTFVNSADMLNQA 589

BLAST of Csor.00g148620 vs. TAIR 10
Match: AT4G18350.1 (nine-cis-epoxycarotenoid dioxygenase 2 )

HSP 1 Score: 811.6 bits (2095), Expect = 4.1e-235
Identity = 382/515 (74.17%), Postives = 451/515 (87.57%), Query Frame = 0

Query: 74  NFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQPVKHNLPVTGTIP 133
           N  QK+A+IA+D AE AL+SHE   PLPKTADPRVQI+GN++PVPE  V+ NL V GTIP
Sbjct: 70  NIFQKAAAIAIDAAERALISHEQDSPLPKTADPRVQIAGNYSPVPESSVRRNLTVEGTIP 129

Query: 134 DCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRFTQTQRLAQEQQLG 193
           DCI+GVY+RNGANP+FEPTAGHHLFDGDGMVHAV I +GSASYACRFT+T+RL QE++LG
Sbjct: 130 DCIDGVYIRNGANPMFEPTAGHHLFDGDGMVHAVKITNGSASYACRFTKTERLVQEKRLG 189

Query: 194 RPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDL 253
           RP+FPKAIGELHGHSGIARL+LFYARG+ GL++++ G GVANAGLVYFN+RLLAMSEDDL
Sbjct: 190 RPVFPKAIGELHGHSGIARLMLFYARGLCGLINNQNGVGVANAGLVYFNNRLLAMSEDDL 249

Query: 254 PYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYDVVRKPYLKYFRFS 313
           PY ++IT +GDL+TVGRYDF+ QL S MIAHPK+DPV++EL+ALSYDVV+KPYLKYFRFS
Sbjct: 250 PYQLKITQTGDLQTVGRYDFDGQLKSAMIAHPKLDPVTKELHALSYDVVKKPYLKYFRFS 309

Query: 314 PDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGGSPVIYDKNKKSRF 373
           PDG+KS ++EIPL+TPTM+HDFAITENFVVIPDQQVVFKL EM+ G SPV++D  K SR 
Sbjct: 310 PDGVKSPELEIPLETPTMIHDFAITENFVVIPDQQVVFKLGEMISGKSPVVFDGEKVSRL 369

Query: 374 GILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECDENLK 433
           GI+PK+AT +  +IWV SP+TFCFHLWNAWE PETEE+VVIGSCM+P DSIFNE DE+L+
Sbjct: 370 GIMPKDATEASQIIWVNSPETFCFHLWNAWESPETEEIVVIGSCMSPADSIFNERDESLR 429

Query: 434 SVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYAYLAIAEPWPKVSG 493
           SVLSEIR+NLRT K+TRR ++ +  E VNLE GMVNRNRLGRKTR+A+LAIA PWPKVSG
Sbjct: 430 SVLSEIRINLRTRKTTRRSLLVN--EDVNLEIGMVNRNRLGRKTRFAFLAIAYPWPKVSG 489

Query: 494 FAKVDLVTGEVKKHIYGGRKFGGEPFFLQ-KEGNSGEKEDEGYILAFVHDERTWKSELQI 553
           FAKVDL TGE+KK+IYGG K+GGEPFFL    GN  E ED+GYI   VHDE T  SELQI
Sbjct: 490 FAKVDLCTGEMKKYIYGGEKYGGEPFFLPGNSGNGEENEDDGYIFCHVHDEETKTSELQI 549

Query: 554 VNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQ 588
           +NA++LK+EAT+KLPSRVPYGFHGTFVD+  L +Q
Sbjct: 550 INAVNLKLEATIKLPSRVPYGFHGTFVDSNELVDQ 582

BLAST of Csor.00g148620 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 791.6 bits (2043), Expect = 4.4e-229
Identity = 399/593 (67.28%), Postives = 473/593 (79.76%), Query Frame = 0

Query: 3   ASSPSPPCLDYLGFHNSTKSINGFKNVNLIRQPSNHNAVIHSALHSPSVLHFPNHSPNFH 62
           +SS S P L  L F +ST           +    N  + + S+L SP+    P+     +
Sbjct: 72  SSSSSRPKLQSLSF-SSTLRNKKLVVPCYVSSSVNKKSSVSSSLQSPT-FKPPSWKKLCN 131

Query: 63  D--NLI-KSPNSN--WNFLQKSASIALDMAESALLSHE-LRHPLPKTADPRVQISGNFAP 122
           D  NLI K+ N N   N +Q++A++ LD  E+A++SHE  RHP PKTADP VQI+GNF P
Sbjct: 132 DVTNLIPKTTNQNPKLNPVQRTAAMVLDAVENAMISHERRRHPHPKTADPAVQIAGNFFP 191

Query: 123 VPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASY 182
           VPE+PV HNLPVTGT+P+CI GVY+RNGANPL +P +GHHLFDGDGMVHAV  ++GS SY
Sbjct: 192 VPEKPVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHLFDGDGMVHAVRFDNGSVSY 251

Query: 183 ACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANA 242
           ACRFT+T RL QE++ GRP+FPKAIGELHGH GIA+L+LF  RG+FGLVD   G GVANA
Sbjct: 252 ACRFTETNRLVQERECGRPVFPKAIGELHGHLGIAKLMLFNTRGLFGLVDPTGGLGVANA 311

Query: 243 GLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYA 302
           GLVYFN  LLAMSEDDLPYHV++T +GDLET GRYDF+ QL STMIAHPK+DP +REL+A
Sbjct: 312 GLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQLKSTMIAHPKIDPETRELFA 371

Query: 303 LSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEM 362
           LSYDVV KPYLKYFRF+ DG KS DVEIPL  PTM+HDFAITENFVVIPDQQVVF+L EM
Sbjct: 372 LSYDVVSKPYLKYFRFTSDGEKSPDVEIPLDQPTMIHDFAITENFVVIPDQQVVFRLPEM 431

Query: 363 LKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGS 422
           ++GGSPV+YD+ KKSRFGIL KNA ++  + W+E PD FCFHLWN+WEEPET+EVVVIGS
Sbjct: 432 IRGGSPVVYDEKKKSRFGILNKNAKDASSIQWIEVPDCFCFHLWNSWEEPETDEVVVIGS 491

Query: 423 CMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRK 482
           CMTPPDSIFNE DE L+SVLSEIRLNL+TG+STRRP+IS   EQVNLEAGMVNRN LGRK
Sbjct: 492 CMTPPDSIFNEHDETLQSVLSEIRLNLKTGESTRRPVIS---EQVNLEAGMVNRNLLGRK 551

Query: 483 TRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGE-KEDEGY 542
           TRYAYLA+ EPWPKVSGFAKVDL TGE++K+IYG  K+GGEP FL     SG+ +ED GY
Sbjct: 552 TRYAYLALTEPWPKVSGFAKVDLSTGEIRKYIYGEGKYGGEPLFLP----SGDGEEDGGY 611

Query: 543 ILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA 589
           I+ FVHDE   KSELQ++NA+++K+EATV LPSRVPYGFHGTF+  + L  QA
Sbjct: 612 IMVFVHDEEKVKSELQLINAVNMKLEATVTLPSRVPYGFHGTFISKEDLSKQA 655

BLAST of Csor.00g148620 vs. TAIR 10
Match: AT3G24220.1 (nine-cis-epoxycarotenoid dioxygenase 6 )

HSP 1 Score: 627.5 bits (1617), Expect = 1.1e-179
Identity = 318/565 (56.28%), Postives = 404/565 (71.50%), Query Frame = 0

Query: 30  NLIRQPSNHNAVIHSALHSPSVLHFPN-HSPNFHDNLIKSPNSNWNFLQKSASIALDMAE 89
           +L+ QP N N      ++   +   P+  SP      +K    N N LQK A+  LD  E
Sbjct: 19  HLLPQPKNANISRRILINPFKIPTLPDLTSPVPSPVKLKPTYPNLNLLQKLAATMLDKIE 78

Query: 90  SAL-LSHELRHPLPKTADPRVQISGNFAPVPEQPVKHNLPVTGTIPDCINGVYLRNGANP 149
           S++ +  E   PLPK  DP VQ+SGNFAPV E PV++ L V G IP C+ GVY+RNGANP
Sbjct: 79  SSIVIPMEQNRPLPKPTDPAVQLSGNFAPVNECPVQNGLEVVGQIPSCLKGVYIRNGANP 138

Query: 150 LFEPTAGHHLFDGDGMVHAVTIN-DGSASYACRFTQTQRLAQEQQLGRPIFPKAIGELHG 209
           +F P AGHHLFDGDGM+HAV+I  D   SY+CR+T+T RL QE  LGR +FPK IGELHG
Sbjct: 139 MFPPLAGHHLFDGDGMIHAVSIGFDNQVSYSCRYTKTNRLVQETALGRSVFPKPIGELHG 198

Query: 210 HSGIARLLLFYARGMFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLE 269
           HSG+ARL LF AR   GLVD  +G GVANAG+V+FN RLLAMSEDDLPY V+I   GDLE
Sbjct: 199 HSGLARLALFTARAGIGLVDGTRGMGVANAGVVFFNGRLLAMSEDDLPYQVKIDGQGDLE 258

Query: 270 TVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYDVVRKPYLKYFRFSPDGIKSKDVEIPL 329
           T+GR+ F+ Q+DS++IAHPKVD  + +L+ LSY+V++KP+L+Y +F+  G K++DVEI L
Sbjct: 259 TIGRFGFDDQIDSSVIAHPKVDATTGDLHTLSYNVLKKPHLRYLKFNTCGKKTRDVEITL 318

Query: 330 QTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGGSPVIYDKNKKSRFGILPKNATNSDDL 389
             PTM+HDFAITENFVVIPDQQ+VFKL EM++GGSPVIY K K +RFG+L K      D+
Sbjct: 319 PEPTMIHDFAITENFVVIPDQQMVFKLSEMIRGGSPVIYVKEKMARFGVLSKQDLTGSDI 378

Query: 390 IWVESPDTFCFHLWNAWEEPETEE----VVVIGSCMTPPDSIFNECDENLKSVLSEIRLN 449
            WV+ PD FCFHLWNAWEE  TEE    +VVIGSCM+PPD+IF+E  E  +  LSEIRLN
Sbjct: 379 NWVDVPDCFCFHLWNAWEE-RTEEGDPVIVVIGSCMSPPDTIFSESGEPTRVELSEIRLN 438

Query: 450 LRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYAYLAIAEPWPKVSGFAKVDLVTG 509
           +RT +S R+ I++     VNLEAG +NR+ +GRK+++ Y+AIA+PWPK SG AKVD+  G
Sbjct: 439 MRTKESNRKVIVTG----VNLEAGHINRSYVGRKSQFVYIAIADPWPKCSGIAKVDIQNG 498

Query: 510 EVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFVHDERTWKSELQIVNAMDLKVEA 569
            V +  YG  +FGGEP F+  EG    +ED+GY++ FV DE   +SE  +V+A D+K  A
Sbjct: 499 TVSEFNYGPSRFGGEPCFV-PEGEG--EEDKGYVMGFVRDEEKDESEFVVVDATDMKQVA 558

Query: 570 TVKLPSRVPYGFHGTFVDAKHLQNQ 588
            V+LP RVPYGFHGTFV    L+ Q
Sbjct: 559 AVRLPERVPYGFHGTFVSENQLKEQ 575

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
K4CJJ14.7e-26078.439-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Solanum lycopersicum O... [more]
O240231.6e-25573.619-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Solanum lycopersicum O... [more]
Q9LRR71.2e-24474.239-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana O... [more]
Q9M6E81.8e-24377.139-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris OX=... [more]
Q9C6Z11.3e-24172.61Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Arabidopsis t... [more]
Match NameE-valueIdentityDescription
KAG6581433.10.0100.009-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic, partial [Cucurbita argyr... [more]
XP_022926148.10.099.839-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucurbita moschata][more]
XP_022978939.10.099.499-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucurbita maxima][more]
XP_023543156.10.099.329-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucurbita pepo subs... [more]
XP_038883946.10.087.179-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1EH850.099.839-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita moschat... [more]
A0A6J1IVE00.099.499-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita maxima ... [more]
A0A2Z4GT570.099.329-cis-epoxycarotenoid dioxygenase OS=Cucurbita pepo OX=3663 PE=2 SV=1[more]
A0A1L5JHV50.086.369-cis-epoxycarotenoid dioxygenase NCED2 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1[more]
A0A1S3AZU00.085.599-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucumis melo OX=3... [more]
Match NameE-valueIdentityDescription
AT3G14440.18.8e-24674.23nine-cis-epoxycarotenoid dioxygenase 3 [more]
AT1G30100.19.1e-24372.61nine-cis-epoxycarotenoid dioxygenase 5 [more]
AT4G18350.14.1e-23574.17nine-cis-epoxycarotenoid dioxygenase 2 [more]
AT1G78390.14.4e-22967.28nine-cis-epoxycarotenoid dioxygenase 9 [more]
AT3G24220.11.1e-17956.28nine-cis-epoxycarotenoid dioxygenase 6 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004294Carotenoid oxygenasePFAMPF03055RPE65coord: 115..580
e-value: 2.5E-116
score: 389.6
IPR004294Carotenoid oxygenasePANTHERPTHR10543BETA-CAROTENE DIOXYGENASEcoord: 62..588
NoneNo IPR availablePANTHERPTHR10543:SF1239-CIS-EPOXYCAROTENOID DIOXYGENASE NCED5, CHLOROPLASTIC-RELATEDcoord: 62..588

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g148620.m01Csor.00g148620.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016121 carotene catabolic process
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0010436 carotenoid dioxygenase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen