Csor.00g147140 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g147140
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	tRNA-5-taurinomethyluridine 2-sulfurtransferase
Location	Csor_Chr20: 369970 .. 373993 (-)
RNA-Seq Expression	Csor.00g147140
Synteny	Csor.00g147140

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTGAGACTGAGAGTGAACCCCGTTTGGTGCACTCTTCCGATCTCCCATTTCACTTCTCTTCCCCTTCGCCCCTCAATTCTCAGACCTGTTCTCCGCCGCCACAGGCCTCGACTCTTCCTCACCCGATTCTCCGCCTCCTCTGCTGTGCATAAAGCCTCGGAATCTTCTTCAGCTAAAGGCCTCTCGGATGGCGACTTGGAGCCTTTCCTCTCATGTTCGATGCCTCGCTATCCGCTCAAAGTTGCTGTTCTTCTTAGCGGTGGCGTCGATAGCAGCGTCGCCCTCCGCCTCCTCCACGCTGCTGGCCACTCCTGCACTGCCTTCTATCTCAAGATATGGTTTCAGGTGCGTACTCTTTGTTCCTTTAATGGAACAGATCATATGCTTCATGTGTTTTCTTGTGAAGTAAGGAAGCTGATATTGGGAATGCTGGCGCAATCGGTGTTTAGGATTATAATTAATGACGAGCGTGGACGTGTTTCTGCTTTGCTATGTATATGATATGTATGTTCTTGGGCAGAGGACAATAGCCTTTTCATGTGACCGCAGTTTTGATGGATTTTGTTTGCGTAGGAAGACTTCGAGAATTTTTGGTCAGAATGCCCATGGGACGATGATTTGAAGTATGCTAAAGCTGTTTGTGATCAGGTTTGTCACTATTTTAGCTTTATGAATCCATCTAGACTTGCAAAGTTTTGAGATTGTTTGGATTGATTTTCTAAGGTCTTAAAAACACTTATTTTATATTTAGAAGCACTTTTTTTTAGTGCATAGGAAGTTAATCCAAACAGACTTTTAATTTGGAAAAAAAAAAAAAAAAATCCAAATTGAACGTAGTCCAAGATACCTATAAGTTATTTCACCACATGATGCTAAGCGTAGTTATAGGTTCTGCGTGTGGGGAGAATCTGTCTTATTTCCTGCCGTCGTTTGTGCTGAAGAGAGAATTAGAACTGAAAATGTACACTGGTTTTCAGGTTGATGTACCATTGGAAGTGGTTCATTTGACAGAGGAATATTGGAGGAACGTGGTAAGTGCCTTTACAATTACAAAATTTTATTCTGTAACTTTGTTGGATAACTTAATGATGTACTACATCAAACCAATTTGGTTATGGTTGTGCTGGCTGACCATCTTGCTCATGTTTCTTTCTAACAGGACGAAATAGTTTTGGGTTAGTTTTTCTGTCTGATGAATATTGTTTAGTTCATGTTTTTAGGTTTCTTATATAATTGAAGAATATCGATGTGGCCGTACTCCAAACCCTGATGTTCTTTGTAACACAAGAATAAAGTTTGGTATGTTCATTAAATTTACCTGAATGGACTTTATAATTTTTTTTGCTATATTGTCAAGTTTTTGGTTATGTCTATGATGTTTTTTGTATTAATATAGTTTATGGCATTCCCTGCTTTTTCCAGGTGCATTCATGGATGCAATAAGCAATATGGAGTTTAATTATGTTGCTTCAGGGCATTATGCGAATGTTATTCATCCATCTGCTGATCAAATGGAAAAACCTTCTGTTTTAGAACTATCCAAGGACATGGTATCTACAAATGGTTGCATTGGCCTTTATTTCATTCTACCCTATCCAATAGGGAGTATCAATCATAACTTGCTTAACTTCAATATTTCGCAGTCGATTGTTTTCATGTTGACTTTTGCCTATATTCTGTGCATGACAGTTAGTGATGCCATTAGTAATTCAACTTGAAGTCTCATATATTACTCTGTGGCATAAATATGTTGATGCCATTGTCGAACACATTTGTCTCTTCCTTGTTCCTTCTTGCTCCAAACTAAAGATTTCTTGTTTCTCTTCACTATTTTTGTTTTCTTAGGTCAAAGATCAAACATATTTTCTGTCGCATCTCTCGCAGAACCAGCTTAAAAGGCTTTTATTCCCTCTTGGTTGTATTCCGAAGGTTTGTTTAAGCTTACTTTACAGCATTTATTATCATGGGTTTTTAAGATATCTCTTACGTTTCAGACTTCAAGAGTGTAATCTGAGAGCTTCAGCTATTGTTGGTCTGATTTTGTACTGGCCTTTTAGGCTTCTACAGACATAAACACCTAGCGGCTAACATTGGTTTCATTCAGTTGAGGAATTTATGTATTTAATAGAAGTTAAAAAAATTCTATTACATAATTTCATAAGCCAATTGGTGTAGATATCATTTTAAAGCTATTGAGTTTTAAACTATTCTTGGGTGCAAGAACTTATATAAATGCTTCAAATAGTAAGTTCATATCATGAATTGGATCTTGCTCCTATCTCAACTCTGGCTACTTTCTTTCATCTAGTGCATACTTGTGTGCTTGTGATCTTGTTAATATGTTTTCCTTGAGTTGTAACTTCAGCCATATTTTTCCCTCTCAGCCTTATATTCCCTCAGCAATTATCTGCAGGATGAGGTTCGCAAACTTGCTGCAAAATTCAACTTACCAAACAAAGACAGAAAGGATTCACAAGGAATATGTTTCCTTGGGAAGGTAGTTTCGGATGTGATGATTTATACATCTATTTTACTTGGAGGACTGAATGCTTTCTCAAAATTCATTCGTCAATGCCAATATTTCTAATATTTTAGTGTTGCCATTTTATATTTTAAAAATATCCTTCTACTTGTTATCACTATTTAAAATGACAGAAGCTAGCTCAAAGAATTTAGTATAAAATTTGCTACTGTTGTAGAATTTTTCTGATGGCTAAACTTTCCCCGAGCTGGGTTTTTATTTCCTTATTCTTCATGACAGTGAAAAAAATCTTGTTCATCCAGCTTCAGAAGATGGGTCTCTGTGTAAGGTATCTTGCGGTACAATTAATGCAGAATCTGTTTCTTTGATGTGTAGATAAAATTTAGTGAATTCGTGGCGAGACATATTGGGGAGGACGAAGGTGTGATATTGGAGGCAGAGTCGGGAGATTTTCTTGGCAAACATCGAGGGTTTTGGTTTTACACGATTGGCCAACGCCAAGGCCTAAAACTTCCTGGAGGACCTTGGTATGCCTTTTTAGTTTATTTTCAAGAATAAGTTCAATGTTCCCAGCTGTTTAGCGGTTTATGATCCCTTCCAAATCTTCAAGAACATCTTAAATTCTACTTGGTAGGGTAGATTTGAACGTTTCAGGGTGCCAGTTTAGTTTTAAATAACTAATCACGTTAGATTATCTCCAATTTGTTGCTTCTCAAGTTAATATTCTTTTGTTGTGTCTCTTCAGCCTTTATGCATTGGTCTAATGCAGGTATGTTGTGGAGAAAGATATTAGGAACAATGTAGTATTTGTTTCGAGAAATTATTTCTCACTTGACAAAAGAAGACGGTTGTTTCGAGTTGGATCATTGAAATGGCTTAGTGGGTTGGTTCCAGACCAATTTACTGAGCTTCAATGTAAGGTAAAGTTTCAGCTTCCATCTCTCATATTTCTCGCTAGTTTCACTACTTCAAGTTGGCCCGCCTGTAACTGTTCAACAGGAGTCAGTTTATTCGTGCAAATTTGTGTTTGAACTGATGGCAGTTCCAGTTCCCCCTTATTTGATGATTTTATACAATTGTTAGGTCCGACATGGCCCGAGTTTCTATAGTTGCAATTTGACAATGGAACGCAATGAAGGTAGTGATGAAGATGTTGCAGTTGTCCATCTAAGCGAAGATGACCAAGGCCTTGCAGCAGGCCAGTTTGCAGCCTTTTATGAGGGAAGGAGATGCATTGGGTCGGGTGTGATTTTAGAATCTTGGGATGATCAGGGGTTTCCTGTATGTGCAAAGGCTCTTGAAATTGCACAAATGGAAGATAAGTCGAAGCTTGGTAAACCAATAAAGATAAAAGTTAAACCAGAGAATGCTTCGAAAGAATGTGAACCTAAAGATGATACAAAAATACACGGCGTGTTAAAGAATCAACAAACCACTGTGGTTGAGCAAACCGGAGAAGTGGCTTCGGATGAAGCACTCACCACAGCCCCAATGAAATGGCTTGGAAGCCTTCGGAAGAAGCTGCTGCAGGTATTTTAA

mRNA sequence

ATGCTGAGACTGAGAGTGAACCCCGTTTGGTGCACTCTTCCGATCTCCCATTTCACTTCTCTTCCCCTTCGCCCCTCAATTCTCAGACCTGTTCTCCGCCGCCACAGGCCTCGACTCTTCCTCACCCGATTCTCCGCCTCCTCTGCTGTGCATAAAGCCTCGGAATCTTCTTCAGCTAAAGGCCTCTCGGATGGCGACTTGGAGCCTTTCCTCTCATGTTCGATGCCTCGCTATCCGCTCAAAGTTGCTGTTCTTCTTAGCGGTGGCGTCGATAGCAGCGTCGCCCTCCGCCTCCTCCACGCTGCTGGCCACTCCTGCACTGCCTTCTATCTCAAGATATGGTTTCAGGAAGACTTCGAGAATTTTTGGTCAGAATGCCCATGGGACGATGATTTGAAGTATGCTAAAGCTGTTTGTGATCAGGTTGATGTACCATTGGAAGTGGTTCATTTGACAGAGGAATATTGGAGGAACGTGGTTTCTTATATAATTGAAGAATATCGATGTGGCCGTACTCCAAACCCTGATGTTCTTTGTAACACAAGAATAAAGTTTGGTGCATTCATGGATGCAATAAGCAATATGGAGTTTAATTATGTTGCTTCAGGGCATTATGCGAATGTTATTCATCCATCTGCTGATCAAATGGAAAAACCTTCTGTTTTAGAACTATCCAAGGACATGGTCAAAGATCAAACATATTTTCTGTCGCATCTCTCGCAGAACCAGCTTAAAAGGCTTTTATTCCCTCTTGGTTGTATTCCGAAGGATGAGGTTCGCAAACTTGCTGCAAAATTCAACTTACCAAACAAAGACAGAAAGGATTCACAAGGAATATGTTTCCTTGGGAAGATAAAATTTAGTGAATTCGTGGCGAGACATATTGGGGAGGACGAAGGTGTGATATTGGAGGCAGAGTCGGGAGATTTTCTTGGCAAACATCGAGGGTTTTGGTTTTACACGATTGGCCAACGCCAAGGCCTAAAACTTCCTGGAGGACCTTGGTATGTTGTGGAGAAAGATATTAGGAACAATGTAGTATTTGTTTCGAGAAATTATTTCTCACTTGACAAAAGAAGACGGTTGTTTCGAGTTGGATCATTGAAATGGCTTAGTGGGTTGGTTCCAGACCAATTTACTGAGCTTCAATGTAAGGTCCGACATGGCCCGAGTTTCTATAGTTGCAATTTGACAATGGAACGCAATGAAGGTAGTGATGAAGATGTTGCAGTTGTCCATCTAAGCGAAGATGACCAAGGCCTTGCAGCAGGCCAGTTTGCAGCCTTTTATGAGGGAAGGAGATGCATTGGGTCGGGTGTGATTTTAGAATCTTGGGATGATCAGGGGTTTCCTGTATGTGCAAAGGCTCTTGAAATTGCACAAATGGAAGATAAGTCGAAGCTTGGTAAACCAATAAAGATAAAAGTTAAACCAGAGAATGCTTCGAAAGAATGTGAACCTAAAGATGATACAAAAATACACGGCGTGTTAAAGAATCAACAAACCACTGTGGTTGAGCAAACCGGAGAAGTGGCTTCGGATGAAGCACTCACCACAGCCCCAATGAAATGGCTTGGAAGCCTTCGGAAGAAGCTGCTGCAGGTATTTTAA

Coding sequence (CDS)

ATGCTGAGACTGAGAGTGAACCCCGTTTGGTGCACTCTTCCGATCTCCCATTTCACTTCTCTTCCCCTTCGCCCCTCAATTCTCAGACCTGTTCTCCGCCGCCACAGGCCTCGACTCTTCCTCACCCGATTCTCCGCCTCCTCTGCTGTGCATAAAGCCTCGGAATCTTCTTCAGCTAAAGGCCTCTCGGATGGCGACTTGGAGCCTTTCCTCTCATGTTCGATGCCTCGCTATCCGCTCAAAGTTGCTGTTCTTCTTAGCGGTGGCGTCGATAGCAGCGTCGCCCTCCGCCTCCTCCACGCTGCTGGCCACTCCTGCACTGCCTTCTATCTCAAGATATGGTTTCAGGAAGACTTCGAGAATTTTTGGTCAGAATGCCCATGGGACGATGATTTGAAGTATGCTAAAGCTGTTTGTGATCAGGTTGATGTACCATTGGAAGTGGTTCATTTGACAGAGGAATATTGGAGGAACGTGGTTTCTTATATAATTGAAGAATATCGATGTGGCCGTACTCCAAACCCTGATGTTCTTTGTAACACAAGAATAAAGTTTGGTGCATTCATGGATGCAATAAGCAATATGGAGTTTAATTATGTTGCTTCAGGGCATTATGCGAATGTTATTCATCCATCTGCTGATCAAATGGAAAAACCTTCTGTTTTAGAACTATCCAAGGACATGGTCAAAGATCAAACATATTTTCTGTCGCATCTCTCGCAGAACCAGCTTAAAAGGCTTTTATTCCCTCTTGGTTGTATTCCGAAGGATGAGGTTCGCAAACTTGCTGCAAAATTCAACTTACCAAACAAAGACAGAAAGGATTCACAAGGAATATGTTTCCTTGGGAAGATAAAATTTAGTGAATTCGTGGCGAGACATATTGGGGAGGACGAAGGTGTGATATTGGAGGCAGAGTCGGGAGATTTTCTTGGCAAACATCGAGGGTTTTGGTTTTACACGATTGGCCAACGCCAAGGCCTAAAACTTCCTGGAGGACCTTGGTATGTTGTGGAGAAAGATATTAGGAACAATGTAGTATTTGTTTCGAGAAATTATTTCTCACTTGACAAAAGAAGACGGTTGTTTCGAGTTGGATCATTGAAATGGCTTAGTGGGTTGGTTCCAGACCAATTTACTGAGCTTCAATGTAAGGTCCGACATGGCCCGAGTTTCTATAGTTGCAATTTGACAATGGAACGCAATGAAGGTAGTGATGAAGATGTTGCAGTTGTCCATCTAAGCGAAGATGACCAAGGCCTTGCAGCAGGCCAGTTTGCAGCCTTTTATGAGGGAAGGAGATGCATTGGGTCGGGTGTGATTTTAGAATCTTGGGATGATCAGGGGTTTCCTGTATGTGCAAAGGCTCTTGAAATTGCACAAATGGAAGATAAGTCGAAGCTTGGTAAACCAATAAAGATAAAAGTTAAACCAGAGAATGCTTCGAAAGAATGTGAACCTAAAGATGATACAAAAATACACGGCGTGTTAAAGAATCAACAAACCACTGTGGTTGAGCAAACCGGAGAAGTGGCTTCGGATGAAGCACTCACCACAGCCCCAATGAAATGGCTTGGAAGCCTTCGGAAGAAGCTGCTGCAGGTATTTTAA

Protein sequence

MLRLRVNPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESSSAKGLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFNYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEGSDEDVAVVHLSEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPENASKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF

Homology

[Close]

BLAST of Csor.00g147140 vs. ExPASy Swiss-Prot
Match: O51625 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680) OX=224326 GN=mnmA PE=3 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 2.6e-100
Identity = 169/365 (46.30%), Postives = 249/365 (68.22%), Query Frame = 0


Query: 80  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 139
           +K+AVLLSGGVDSSVAL R+++    +   +YLKIW +++  ++   CPW +DL Y +A+
Sbjct: 1   MKIAVLLSGGVDSSVALYRIINKGYSNIKCYYLKIWVEDEL-SYIGNCPWQEDLNYVEAI 60

Query: 140 CDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFN 199
           C++ +VP E+++  +EY+  VVSY IEE + G TP+PD+ CN RIKFGAF + I N +++
Sbjct: 61  CNKFNVPYEIINFQKEYYNKVVSYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NSQYD 120

Query: 200 YVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 259
            V +GHYA +        E   +L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYAKI-----QIKESKFLLKQAKDKIKDQSYFLSHLSQKQMSKLYFPLGTLLKSE 180

Query: 260 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 319
           VR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKNINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGIHNGYW 240

Query: 320 FYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRRRLFRVGSLKWLSGLVPDQ 379
           F+T+GQR+G+KL  GPW+V+EKD+  N++++S N   L + +R F V  + W++   P  
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWIND-TPTN 300

Query: 380 FTELQCKVRHGPSFYSCNLTMERNEGSDEDVAVVHLSEDDQGLAAGQFAAFYEGRRCIGS 439
           F   + K+RHG   YSC L +  N     ++  + L++ DQG++ GQFA FY+   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLKLITN-----NLMEISLNKKDQGISPGQFAIFYKNTECLGG 352

Query: 440 GVILE 444
             I +
Sbjct: 361 AKIFK 352

[Close]

BLAST of Csor.00g147140 vs. ExPASy Swiss-Prot
Match: B7J2N7 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ZS7) OX=445985 GN=mnmA PE=3 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 2.6e-100
Identity = 169/365 (46.30%), Postives = 249/365 (68.22%), Query Frame = 0


Query: 80  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 139
           +K+AVLLSGGVDSSVAL R+++    +   +YLKIW +++  ++   CPW +DL Y +A+
Sbjct: 1   MKIAVLLSGGVDSSVALYRIINKGYSNIKCYYLKIWVEDEL-SYIGNCPWQEDLNYVEAI 60

Query: 140 CDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFN 199
           C++ +VP E+++  +EY+  VVSY IEE + G TP+PD+ CN RIKFGAF + I N +++
Sbjct: 61  CNKFNVPYEIINFQKEYYNKVVSYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NSQYD 120

Query: 200 YVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 259
            V +GHYA +        E   +L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYAKI-----QIKESKFLLKQAKDKIKDQSYFLSHLSQKQISKLYFPLGTLLKSE 180

Query: 260 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 319
           VR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKNINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGIHNGYW 240

Query: 320 FYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRRRLFRVGSLKWLSGLVPDQ 379
           F+T+GQR+G+KL  GPW+V+EKD+  N++++S N   L + +R F V  + W++   P  
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWIND-TPTN 300

Query: 380 FTELQCKVRHGPSFYSCNLTMERNEGSDEDVAVVHLSEDDQGLAAGQFAAFYEGRRCIGS 439
           F   + K+RHG   YSC L +  N     ++  + L++ DQG++ GQFA FY+   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLKLITN-----NLMEISLNKKDQGISPGQFAIFYKNTECLGG 352

Query: 440 GVILE 444
             I +
Sbjct: 361 AKIFK 352

[Close]

BLAST of Csor.00g147140 vs. ExPASy Swiss-Prot
Match: Q660I8 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia bavariensis (strain ATCC BAA-2496 / DSM 23469 / PBi) OX=290434 GN=mnmA PE=3 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 1.7e-99
Identity = 167/365 (45.75%), Postives = 249/365 (68.22%), Query Frame = 0


Query: 80  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 139
           +K+AVLLSGGVDSS+AL ++++    +   +YLKIW +++  ++   CPW +DL Y +AV
Sbjct: 1   MKIAVLLSGGVDSSIALYKVINKGYTNIKCYYLKIWLEDEL-SYIGNCPWQEDLNYVEAV 60

Query: 140 CDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFN 199
           C++ ++P E+++  +EY+  VV Y IEE + G TP+PD+ CN RIKFGAF + I N +++
Sbjct: 61  CNKFNIPYEIINFQKEYYNKVVIYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NEQYD 120

Query: 200 YVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 259
            V +GHYA +        EK   L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYARI-----QIKEKKFFLKQAKDKIKDQSYFLSHLSQEQMSKLYFPLGTLFKSE 180

Query: 260 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 319
           VR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKSINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIVEKETGKIIGIHNGYW 240

Query: 320 FYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRRRLFRVGSLKWLSGLVPDQ 379
           F+T+GQR+G+KL  GPW+V+EKD+  N++++S N   L + +R F V  + W++G+ P  
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWINGM-PSD 300

Query: 380 FTELQCKVRHGPSFYSCNLTMERNEGSDEDVAVVHLSEDDQGLAAGQFAAFYEGRRCIGS 439
           F   + K+RHG   YSC L +  N     ++  + L++ D G++ GQFA FY+   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLRLITN-----NLIEIFLNKKDHGISPGQFAIFYKNTECLGG 352

Query: 440 GVILE 444
             I +
Sbjct: 361 AKIFK 352

[Close]

BLAST of Csor.00g147140 vs. ExPASy Swiss-Prot
Match: Q0SMH1 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia afzelii (strain PKo) OX=390236 GN=mnmA PE=3 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 8.5e-99
Identity = 167/365 (45.75%), Postives = 247/365 (67.67%), Query Frame = 0


Query: 80  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 139
           +K+AVLLSGGVDSSVAL R+++    S   +YLKIW +++  ++   CPW +DL Y +AV
Sbjct: 1   MKIAVLLSGGVDSSVALYRIINKGYTSIKCYYLKIWLEDEL-SYIGSCPWQEDLNYVEAV 60

Query: 140 CDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFN 199
           C + +VP E+++  +EY+  VVSY I+E + G TP+PD+ CN RIKFGAF + I N +++
Sbjct: 61  CSKFNVPYEIINFQKEYYNKVVSYTIKELKNGNTPSPDIFCNQRIKFGAFFEKI-NEQYD 120

Query: 200 YVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 259
            V +GHYA +        +K   L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYAKI-----QTKDKKFFLKQAKDKIKDQSYFLSHLSQKQMSKLYFPLGTLLKSE 180

Query: 260 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 319
           VR++A   NLPNK+RKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKNINLPNKNRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGTHNGYW 240

Query: 320 FYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRRRLFRVGSLKWLSGLVPDQ 379
           F+T+GQR+G+KL  GPW+V+EKD+  N++++S N     + +R F V  + W++G  P  
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYSKQAKRKFLVHEIHWING-TPSD 300

Query: 380 FTELQCKVRHGPSFYSCNLTMERNEGSDEDVAVVHLSEDDQGLAAGQFAAFYEGRRCIGS 439
           F   + K+RHG   YSC L +       +++  + L++ D G++ GQFA FY+   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLKL-----IAKNLIEISLNKKDYGISPGQFAIFYKNTECLGG 352

Query: 440 GVILE 444
             I +
Sbjct: 361 AKIFK 352

[Close]

BLAST of Csor.00g147140 vs. ExPASy Swiss-Prot
Match: B2S125 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia hermsii (strain HS1 / DAH) OX=314723 GN=mnmA PE=3 SV=2)

HSP 1 Score: 356.7 bits (914), Expect = 4.6e-97
Identity = 168/366 (45.90%), Postives = 246/366 (67.21%), Query Frame = 0


Query: 80  LKVAVLLSGGVDSSVALRLLHAAGH-SCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 139
           +K+AVLLSGGVDSSVAL  +   G+ +   +YLKIW +++  ++  ECPW++D+ Y +AV
Sbjct: 1   MKIAVLLSGGVDSSVALYTMIQKGYKNIKCYYLKIWLEDEL-SYIGECPWEEDINYVEAV 60

Query: 140 CDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFN 199
           C + +VP E++ L +EY++ VV+Y IEE + G TP+PD+ CN RIKFGAF D I N  ++
Sbjct: 61  CTKFNVPYEIISLQDEYYKRVVTYAIEELKIGNTPSPDIFCNQRIKFGAFFDKI-NEHYD 120

Query: 200 YVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 259
            + +GHYA +     +      +L+ +KD +KDQ+YFLSHLS+ Q+ +L FPLG + K E
Sbjct: 121 LIVTGHYAKI-----ENKNNHYILKQAKDKIKDQSYFLSHLSREQISKLHFPLGDLLKTE 180

Query: 260 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 319
           +R++A K +LPNK+RKDSQGICFLGKIK+ EF+  H+GE +G I+E E+G  LG H G+W
Sbjct: 181 IRQIAHKIDLPNKNRKDSQGICFLGKIKYDEFIKYHLGELKGNIIEQETGKILGTHNGYW 240

Query: 320 FYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRRRLFRVGSLKWLSGLVPDQ 379
           F+TIGQR+G+KL  GPW+V EKDI+NN++++S +   L + +  F V    W++   P  
Sbjct: 241 FFTIGQRKGIKLSHGPWFVTEKDIQNNIIYISNSVNYLAQGKHQFLVHKTNWIN--KPVN 300

Query: 380 FTELQCKVRHGPSFYSCNLTMERNEGSDEDVAVVHLSEDDQGLAAGQFAAFYEGRRCIGS 439
             +L  K+RHG     C + M +N     D+  V L E D G++ GQF  FY+   C+G 
Sbjct: 301 NIDLSAKIRHGEKKIKCKIEMLKN-----DIIKVDLEEKDYGISPGQFCIFYQEDECLGG 352

Query: 440 GVILES 445
             IL++
Sbjct: 361 AQILQT 352

[Close]

BLAST of Csor.00g147140 vs. NCBI nr
Match: KAG6570391.1 (hypothetical protein SDJN03_29306, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1095 bits (2832), Expect = 0.0
Identity = 536/536 (100.00%), Postives = 536/536 (100.00%), Query Frame = 0


Query: 1   MLRLRVNPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESSSAK 60
           MLRLRVNPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESSSAK
Sbjct: 1   MLRLRVNPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESSSAK 60

Query: 61  GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 120
           GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE
Sbjct: 61  GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 120

Query: 121 NFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN 180
           NFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN
Sbjct: 121 NFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN 180

Query: 181 TRIKFGAFMDAISNMEFNYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSHLS 240
           TRIKFGAFMDAISNMEFNYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSHLS
Sbjct: 181 TRIKFGAFMDAISNMEFNYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSHLS 240

Query: 241 QNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEG 300
           QNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEG
Sbjct: 241 QNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEG 300

Query: 301 VILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRR 360
           VILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRR
Sbjct: 301 VILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRR 360

Query: 361 RLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEGSDEDVAVVHLSEDDQG 420
           RLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEGSDEDVAVVHLSEDDQG
Sbjct: 361 RLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEGSDEDVAVVHLSEDDQG 420

Query: 421 LAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPEN 480
           LAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPEN
Sbjct: 421 LAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPEN 480

Query: 481 ASKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536
           ASKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF
Sbjct: 481 ASKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536

[Close]

BLAST of Csor.00g147140 vs. NCBI nr
Match: XP_022944568.1 (uncharacterized protein LOC111448983 [Cucurbita moschata])

HSP 1 Score: 1087 bits (2812), Expect = 0.0
Identity = 532/536 (99.25%), Postives = 535/536 (99.81%), Query Frame = 0


Query: 1   MLRLRVNPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESSSAK 60
           MLRLRVNPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESSSAK
Sbjct: 1   MLRLRVNPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESSSAK 60

Query: 61  GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 120
           GLSDGDLEPFLSCSMPR+PLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE
Sbjct: 61  GLSDGDLEPFLSCSMPRHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 120

Query: 121 NFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN 180
           NFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN
Sbjct: 121 NFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN 180

Query: 181 TRIKFGAFMDAISNMEFNYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSHLS 240
           TRIKFGAFMDAISNMEF+YVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSHLS
Sbjct: 181 TRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSHLS 240

Query: 241 QNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEG 300
           QNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEG
Sbjct: 241 QNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEG 300

Query: 301 VILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRR 360
           VILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRR
Sbjct: 301 VILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRR 360

Query: 361 RLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEGSDEDVAVVHLSEDDQG 420
           RLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNE SDEDVAVVHLSEDDQG
Sbjct: 361 RLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVHLSEDDQG 420

Query: 421 LAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPEN 480
           LAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPEN
Sbjct: 421 LAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPEN 480

Query: 481 ASKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536
           A+KECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF
Sbjct: 481 ATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536

[Close]

BLAST of Csor.00g147140 vs. NCBI nr
Match: XP_023513286.1 (uncharacterized protein LOC111777800 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1082 bits (2798), Expect = 0.0
Identity = 529/536 (98.69%), Postives = 533/536 (99.44%), Query Frame = 0


Query: 1   MLRLRVNPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESSSAK 60
           MLRLRV PVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASES SAK
Sbjct: 1   MLRLRVKPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESYSAK 60

Query: 61  GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 120
           GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE
Sbjct: 61  GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 120

Query: 121 NFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN 180
           NFWSECPWDDDLKYA+AVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN
Sbjct: 121 NFWSECPWDDDLKYAEAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN 180

Query: 181 TRIKFGAFMDAISNMEFNYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSHLS 240
           TRIKFGAFMDAISNMEF+YVASGHYANVIHPSADQM+KPSVLELSKDMVKDQTYFLSHLS
Sbjct: 181 TRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLS 240

Query: 241 QNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEG 300
           QNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEG
Sbjct: 241 QNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEG 300

Query: 301 VILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRR 360
           VILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRR
Sbjct: 301 VILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRR 360

Query: 361 RLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEGSDEDVAVVHLSEDDQG 420
           RLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNE SDEDVAVVHLSEDDQG
Sbjct: 361 RLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVHLSEDDQG 420

Query: 421 LAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPEN 480
           LAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPEN
Sbjct: 421 LAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPEN 480

Query: 481 ASKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536
           A+KECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF
Sbjct: 481 ATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536

[Close]

BLAST of Csor.00g147140 vs. NCBI nr
Match: KAG7010265.1 (mnmA [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1073 bits (2774), Expect = 0.0
Identity = 535/582 (91.92%), Postives = 536/582 (92.10%), Query Frame = 0


Query: 1   MLRLRVNPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESSSAK 60
           MLRLRVNPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESSSAK
Sbjct: 1   MLRLRVNPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESSSAK 60

Query: 61  GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 120
           GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE
Sbjct: 61  GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 120

Query: 121 NFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN 180
           NFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN
Sbjct: 121 NFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN 180

Query: 181 TRIKFGAFMDAISNMEFNYVASGHYANVIHPSADQMEKPSVLELSKDMV----------- 240
           TRIKFGAFMDAISNMEFNYVASGHYANVIHPSADQMEKPSVLELSKDMV           
Sbjct: 181 TRIKFGAFMDAISNMEFNYVASGHYANVIHPSADQMEKPSVLELSKDMVSTNGCIGLYFI 240

Query: 241 -----------------------------------KDQTYFLSHLSQNQLKRLLFPLGCI 300
                                              KDQTYFLSHLSQNQLKRLLFPLGCI
Sbjct: 241 LPYPIGSINHNLLNFNISQSIVFVLTFAYILCMTVKDQTYFLSHLSQNQLKRLLFPLGCI 300

Query: 301 PKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKH 360
           PKDEVRKLAAKFNLPNKDRKDSQGICFLGK+KFSEFVARHIGEDEGVILEAESGDFLGKH
Sbjct: 301 PKDEVRKLAAKFNLPNKDRKDSQGICFLGKVKFSEFVARHIGEDEGVILEAESGDFLGKH 360

Query: 361 RGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRRRLFRVGSLKWLSGL 420
           RGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRRRLFRVGSLKWLSGL
Sbjct: 361 RGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRRRLFRVGSLKWLSGL 420

Query: 421 VPDQFTELQCKVRHGPSFYSCNLTMERNEGSDEDVAVVHLSEDDQGLAAGQFAAFYEGRR 480
           VPDQFTELQCKVRHGPSFYSCNLTMERNEGSDEDVAVVHLSEDDQGLAAGQFAAFYEGRR
Sbjct: 421 VPDQFTELQCKVRHGPSFYSCNLTMERNEGSDEDVAVVHLSEDDQGLAAGQFAAFYEGRR 480

Query: 481 CIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPENASKECEPKDDTKIH 536
           CIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPENASKECEPKDDTKIH
Sbjct: 481 CIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPENASKECEPKDDTKIH 540

[Close]

BLAST of Csor.00g147140 vs. NCBI nr
Match: XP_022985745.1 (uncharacterized protein LOC111483706 [Cucurbita maxima])

HSP 1 Score: 1061 bits (2745), Expect = 0.0
Identity = 521/536 (97.20%), Postives = 528/536 (98.51%), Query Frame = 0


Query: 1   MLRLRVNPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESSSAK 60
           MLRLRV PVWCTLPISHFTSL LRPSILRPVLRRHRPRLF+TRFSASSAVHKASESSSAK
Sbjct: 1   MLRLRVKPVWCTLPISHFTSLTLRPSILRPVLRRHRPRLFITRFSASSAVHKASESSSAK 60

Query: 61  GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 120
           GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE
Sbjct: 61  GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 120

Query: 121 NFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN 180
           NFWSECPWDDDLKYAKAVCD+VDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN
Sbjct: 121 NFWSECPWDDDLKYAKAVCDRVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN 180

Query: 181 TRIKFGAFMDAISNMEFNYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSHLS 240
           TRIKFGAFMDAISNMEF+YVASGHYANVIHPSADQM+KPSVLELSKDMVKDQTYFLSHLS
Sbjct: 181 TRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLS 240

Query: 241 QNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEG 300
           QNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEG
Sbjct: 241 QNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEG 300

Query: 301 VILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRR 360
           VILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRR
Sbjct: 301 VILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRR 360

Query: 361 RLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEGSDEDVAVVHLSEDDQG 420
           R FRVGSLKWLSGLVPDQFT LQCKVRHGPSFYSC LTMER+E SDEDVAVV LSEDDQG
Sbjct: 361 RSFRVGSLKWLSGLVPDQFTGLQCKVRHGPSFYSCILTMERDEDSDEDVAVVLLSEDDQG 420

Query: 421 LAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPEN 480
           LAAGQFAAFY+GRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPEN
Sbjct: 421 LAAGQFAAFYQGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPEN 480

Query: 481 ASKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536
           A+KECE KDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF
Sbjct: 481 ATKECETKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536

[Close]

BLAST of Csor.00g147140 vs. ExPASy TrEMBL
Match: A0A6J1FY63 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucurbita moschata OX=3662 GN=LOC111448983 PE=3 SV=1)

HSP 1 Score: 1087 bits (2812), Expect = 0.0
Identity = 532/536 (99.25%), Postives = 535/536 (99.81%), Query Frame = 0


Query: 1   MLRLRVNPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESSSAK 60
           MLRLRVNPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESSSAK
Sbjct: 1   MLRLRVNPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESSSAK 60

Query: 61  GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 120
           GLSDGDLEPFLSCSMPR+PLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE
Sbjct: 61  GLSDGDLEPFLSCSMPRHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 120

Query: 121 NFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN 180
           NFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN
Sbjct: 121 NFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN 180

Query: 181 TRIKFGAFMDAISNMEFNYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSHLS 240
           TRIKFGAFMDAISNMEF+YVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSHLS
Sbjct: 181 TRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSHLS 240

Query: 241 QNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEG 300
           QNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEG
Sbjct: 241 QNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEG 300

Query: 301 VILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRR 360
           VILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRR
Sbjct: 301 VILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRR 360

Query: 361 RLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEGSDEDVAVVHLSEDDQG 420
           RLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNE SDEDVAVVHLSEDDQG
Sbjct: 361 RLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVHLSEDDQG 420

Query: 421 LAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPEN 480
           LAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPEN
Sbjct: 421 LAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPEN 480

Query: 481 ASKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536
           A+KECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF
Sbjct: 481 ATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536

[Close]

BLAST of Csor.00g147140 vs. ExPASy TrEMBL
Match: A0A6J1JC61 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucurbita maxima OX=3661 GN=LOC111483706 PE=3 SV=1)

HSP 1 Score: 1061 bits (2745), Expect = 0.0
Identity = 521/536 (97.20%), Postives = 528/536 (98.51%), Query Frame = 0


Query: 1   MLRLRVNPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESSSAK 60
           MLRLRV PVWCTLPISHFTSL LRPSILRPVLRRHRPRLF+TRFSASSAVHKASESSSAK
Sbjct: 1   MLRLRVKPVWCTLPISHFTSLTLRPSILRPVLRRHRPRLFITRFSASSAVHKASESSSAK 60

Query: 61  GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 120
           GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE
Sbjct: 61  GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 120

Query: 121 NFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN 180
           NFWSECPWDDDLKYAKAVCD+VDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN
Sbjct: 121 NFWSECPWDDDLKYAKAVCDRVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN 180

Query: 181 TRIKFGAFMDAISNMEFNYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSHLS 240
           TRIKFGAFMDAISNMEF+YVASGHYANVIHPSADQM+KPSVLELSKDMVKDQTYFLSHLS
Sbjct: 181 TRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLS 240

Query: 241 QNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEG 300
           QNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEG
Sbjct: 241 QNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEG 300

Query: 301 VILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRR 360
           VILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRR
Sbjct: 301 VILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRR 360

Query: 361 RLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEGSDEDVAVVHLSEDDQG 420
           R FRVGSLKWLSGLVPDQFT LQCKVRHGPSFYSC LTMER+E SDEDVAVV LSEDDQG
Sbjct: 361 RSFRVGSLKWLSGLVPDQFTGLQCKVRHGPSFYSCILTMERDEDSDEDVAVVLLSEDDQG 420

Query: 421 LAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPEN 480
           LAAGQFAAFY+GRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPEN
Sbjct: 421 LAAGQFAAFYQGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPEN 480

Query: 481 ASKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536
           A+KECE KDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF
Sbjct: 481 ATKECETKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536

[Close]

BLAST of Csor.00g147140 vs. ExPASy TrEMBL
Match: A0A0A0KX16 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis sativus OX=3659 GN=Csa_4G291390 PE=3 SV=1)

HSP 1 Score: 987 bits (2551), Expect = 0.0
Identity = 481/537 (89.57%), Postives = 503/537 (93.67%), Query Frame = 0


Query: 1   MLRLRVNP-VWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESSSA 60
           MLRLRVNP VWC++PISHFTSLPL PSILRP+LRRH PRLFLTRFSASSAVH  S SSS+
Sbjct: 2   MLRLRVNPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSSS 61

Query: 61  KGLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 120
           K LSD D  PFLSCSMP YPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF
Sbjct: 62  KPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 121

Query: 121 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 180
           ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC
Sbjct: 122 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 181

Query: 181 NTRIKFGAFMDAISNMEFNYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSHL 240
           NTRIKFGAFMDAISNMEF+YVASGHYANVIHPSADQM+KPSVLELSKDMVKDQTYFLSHL
Sbjct: 182 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL 241

Query: 241 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 300
           SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE
Sbjct: 242 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 301

Query: 301 GVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKR 360
           GVILEAESGDFLGKHRGFWFYTIGQRQGL+LPGGPWYVVEKDI+NNVVFVSRNYFS DKR
Sbjct: 302 GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR 361

Query: 361 RRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEGSDEDVAVVHLSEDDQ 420
           RR+FRVGSLKWLSG  PDQFTELQCKVRHGPSFYSCNLTME +E   EDVAVVHLSEDDQ
Sbjct: 362 RRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDDQ 421

Query: 421 GLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPE 480
           GLAAGQFAAFY+GR CIGSGVILESWDDQGFPVC KA+E AQMEDKSKLGKPIKIKVKPE
Sbjct: 422 GLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKPE 481

Query: 481 NASKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536
           +  KE + KDDT+IH  L+N++ TVVEQ  EV+ DEA+ T  MKWLGSL+KKLLQ+F
Sbjct: 482 SGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 538

[Close]

BLAST of Csor.00g147140 vs. ExPASy TrEMBL
Match: A0A5A7TDN6 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00570 PE=3 SV=1)

HSP 1 Score: 970 bits (2507), Expect = 0.0
Identity = 473/538 (87.92%), Postives = 497/538 (92.38%), Query Frame = 0


Query: 1   MLRLRVNP-VWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAV-HKASESSS 60
           MLRLRVNP VWC++PISHFTSLPL PSILRP+LR H PRLF TRFSASSAV H  S+SS 
Sbjct: 2   MLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSSL 61

Query: 61  AKGLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120
            K LSD D  PFLSCSMP YPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 62  PKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 121

Query: 121 FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180
           FENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL
Sbjct: 122 FENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 181

Query: 181 CNTRIKFGAFMDAISNMEFNYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSH 240
           CNTRIKFGAFMDAISNMEF+YVASGHYANVIHPSADQM+KPSVLELSKDMVKDQTYFLSH
Sbjct: 182 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 241

Query: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300
           LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED
Sbjct: 242 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 301

Query: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDK 360
           EGVILEAESGDFLGKHRGFWFYT+GQRQGL+LPGGPWYVVEKDI+NNVVFVSRNYFS DK
Sbjct: 302 EGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 361

Query: 361 RRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEGSDEDVAVVHLSEDD 420
           RRR+FRVGSLKWLSG+ PDQFT LQCKVRHGP FYSCNLTME NE   EDVAVVHLSEDD
Sbjct: 362 RRRVFRVGSLKWLSGMPPDQFTGLQCKVRHGPGFYSCNLTMEHNEDGHEDVAVVHLSEDD 421

Query: 421 QGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKP 480
           QGLAAGQFAAFY+GR CIGSGVILESWDDQGFPVC KA+E AQMEDKSKLGKPIKIKVKP
Sbjct: 422 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 481

Query: 481 ENASKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536
           ++   E + KDDTKI    +N++ TVVEQ  +V+ DEA+ T PMKWLGSLRKKLLQ+F
Sbjct: 482 DSGMNESKAKDDTKIQDESRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

[Close]

BLAST of Csor.00g147140 vs. ExPASy TrEMBL
Match: A0A5D3B8G8 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G001100 PE=3 SV=1)

HSP 1 Score: 967 bits (2499), Expect = 0.0
Identity = 471/538 (87.55%), Postives = 497/538 (92.38%), Query Frame = 0


Query: 1   MLRLRVNP-VWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAV-HKASESSS 60
           MLRLRVNP VWC++PISHFTSLPL PSILRP+LR H PRLF TRFSASSAV H  S+SS 
Sbjct: 2   MLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSSL 61

Query: 61  AKGLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120
            K LSD D  PFLSCSMP YPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 62  PKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 121

Query: 121 FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180
           FENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL
Sbjct: 122 FENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 181

Query: 181 CNTRIKFGAFMDAISNMEFNYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSH 240
           CNTRIKFGAFMDAISNMEF+YVASGHYANVIHPSADQM+KPSVLELSKDMVKDQTYFLSH
Sbjct: 182 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 241

Query: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300
           LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED
Sbjct: 242 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 301

Query: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDK 360
           EGVILEAESGDFLGKHRGFWFYT+GQRQGL+LPGGPWYVVEKDI+NNVVFVSRNYFS DK
Sbjct: 302 EGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 361

Query: 361 RRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEGSDEDVAVVHLSEDD 420
           RRR+FRVGSLKWLSG+ PD+FT LQCKVRHGP FYSCNLTME N+   EDVAVVHLSEDD
Sbjct: 362 RRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDD 421

Query: 421 QGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKP 480
           QGLAAGQFAAFY+GR CIGSGVILESWDDQGFPVC KA+E AQMEDKSKLGKPIKIKVKP
Sbjct: 422 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 481

Query: 481 ENASKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536
           ++   E + KDDTKI    +N++ TVVEQ  +V+ DEA+ T PMKWLGSLRKKLLQ+F
Sbjct: 482 DSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

[Close]

BLAST of Csor.00g147140 vs. TAIR 10
Match: AT1G51310.1 (transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases )

HSP 1 Score: 713.4 bits (1840), Expect = 1.4e-205
Identity = 336/420 (80.00%), Postives = 372/420 (88.57%), Query Frame = 0


Query: 67  LEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSEC 126
           ++ +LSCSMP  PL+VAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE FENFW++C
Sbjct: 69  VDQYLSCSMPEKPLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEGFENFWNQC 128

Query: 127 PWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFG 186
           PW+DDLKYAK VC+QVDVPLEVVHLT+EYW  VVSYIIEEYRCGRTPNPDVLCNTRIKFG
Sbjct: 129 PWEDDLKYAKHVCEQVDVPLEVVHLTDEYWERVVSYIIEEYRCGRTPNPDVLCNTRIKFG 188

Query: 187 AFMDAISNMEFNYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSHLSQNQLKR 246
           AFMDAIS+ME++YV SGHYA V+HP ADQ +  SVLELS+DMVKDQTYFLSHLSQ QLKR
Sbjct: 189 AFMDAISDMEYDYVGSGHYAKVVHPPADQNDASSVLELSQDMVKDQTYFLSHLSQTQLKR 248

Query: 247 LLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAE 306
           LLFPLGC+ KDEVRKLA +F+LPNKDRKDSQGICFLGKIKFS+FV RHIGE EG+ILEAE
Sbjct: 249 LLFPLGCVKKDEVRKLATQFDLPNKDRKDSQGICFLGKIKFSDFVCRHIGEMEGIILEAE 308

Query: 307 SGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRRRLFRVG 366
           SGDFLG HRGFWFYTIGQRQGL+LPGGPWYVVEKD +NNVVFVSRNY+S+DKRRR+FRVG
Sbjct: 309 SGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYYSIDKRRRIFRVG 368

Query: 367 SLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEGSDEDVAVVHLSEDDQGLAAGQF 426
           SL+W SG    +  EL+CKVRHGP FYSC+  ME     D DVAVVHL EDDQGLAAGQF
Sbjct: 369 SLRWNSGKPSGKVRELRCKVRHGPGFYSCSFEME----GDGDVAVVHLDEDDQGLAAGQF 428

Query: 427 AAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPENASKECE 486
           AAFYEG  CIGSGVILESWDDQ FPVCAKAL++A +EDK+KLGKP+KI   P  +S E +
Sbjct: 429 AAFYEGTTCIGSGVILESWDDQCFPVCAKALQLAALEDKTKLGKPVKIMTMPTMSSVEAD 484

The following BLAST results are available for this feature:

BLAST of Csor.00g147140 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O51625	2.6e-100	46.30	tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ATCC 35210 / ...	[more]
B7J2N7	2.6e-100	46.30	tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ZS7) OX=44598...	[more]
Q660I8	1.7e-99	45.75	tRNA-specific 2-thiouridylase MnmA OS=Borrelia bavariensis (strain ATCC BAA-2496...	[more]
Q0SMH1	8.5e-99	45.75	tRNA-specific 2-thiouridylase MnmA OS=Borrelia afzelii (strain PKo) OX=390236 GN...	[more]
B2S125	4.6e-97	45.90	tRNA-specific 2-thiouridylase MnmA OS=Borrelia hermsii (strain HS1 / DAH) OX=314...	[more]

BLAST of Csor.00g147140 vs. NCBI nr


Match Name	Identity	Description
KAG6570391.1	100.00	hypothetical protein SDJN03_29306, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022944568.1	99.25	uncharacterized protein LOC111448983 [Cucurbita moschata]	[more]
XP_023513286.1	98.69	uncharacterized protein LOC111777800 [Cucurbita pepo subsp. pepo]	[more]
KAG7010265.1	91.92	mnmA [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022985745.1	97.20	uncharacterized protein LOC111483706 [Cucurbita maxima]	[more]

BLAST of Csor.00g147140 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1FY63	99.25	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1JC61	97.20	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A0A0KX16	89.57	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis sativus OX=3659 GN=Cs...	[more]
A0A5A7TDN6	87.92	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1...	[more]
A0A5D3B8G8	87.55	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1...	[more]

BLAST of Csor.00g147140 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G51310.1	1.4e-205	80.00	transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	2.40.30.10	Translation factors	coord: 360..449 e-value: 1.4E-13 score: 52.3
None	No IPR available	PFAM	PF03054	tRNA_Me_trans	coord: 81..441 e-value: 1.8E-126 score: 421.7
None	No IPR available	PANTHER	PTHR43052	FAMILY NOT NAMED	coord: 53..474
None	No IPR available	SUPERFAMILY	52402	Adenine nucleotide alpha hydrolases-like	coord: 81..326
IPR023382	Adenine nucleotide alpha hydrolase-like domains	GENE3D	2.30.30.280		coord: 294..353 e-value: 1.4E-18 score: 68.0
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 72..293 e-value: 8.4E-85 score: 285.7
IPR004506	tRNA-specific 2-thiouridylase	TIGRFAM	TIGR00420	TIGR00420	coord: 81..441 e-value: 6.3E-110 score: 365.3
IPR004506	tRNA-specific 2-thiouridylase	HAMAP	MF_00144	tRNA_thiouridyl_MnmA	coord: 80..442 score: 33.159828
IPR004506	tRNA-specific 2-thiouridylase	CDD	cd01998	tRNA_Me_trans	coord: 81..441 e-value: 3.04944E-158 score: 452.747

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g147140.m01	Csor.00g147140.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008033	tRNA processing
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0061708	tRNA-5-taurinomethyluridine 2-sulfurtransferase
molecular_function	GO:0000049	tRNA binding
molecular_function	GO:0016783	sulfurtransferase activity
molecular_function	GO:0016740	transferase activity