Csor.00g143240 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g143240
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionlipid droplet-associated hydrolase-like
LocationCsor_Chr03: 8795372 .. 8799226 (+)
RNA-Seq ExpressionCsor.00g143240
SyntenyCsor.00g143240
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCAAGAAGATCTGCAATCGTTTAGTAGAAGGCGTGTGGAGTTTCGTTTATGCAATGTATCAGGGTAATTGATTCTCGTTTATGTTTACTGAAATTTCTCTGGAATTTCGTTTTCTATGAACTAAATCGCCTTGCCCTTTCTGGGTTTTGATAGCTACACCAATGAATTGCTGGAGATTCACTCGGATAATCCATCATTGCACGTATTGTTCATACCTGGAAATCCAGGTGCATTTGGCTTTTTCGATGTGGGTTTTCGATGATTTTGTTGATTTCTACTTAACTTCTGAATGTGGATTGTTTTGGGGTTTGTTCTAAGACAGGGATTATTTCGTTTTATAAACATTTTGTGGAATCATTGTATCAGTTGCTTGGAGGCCGTGTATCTATAACAGGCAAGATCATGAGTTTTTGACTTTGTTCATATCCCTTTCATTTTTTTTCTAAGAATGCATTAAATCTTGTTGTATATTTGAGCTTACACTTTTTCTTGTTCCATTTATGTTCTTTGGGCCTGCTTCTGTCTACTTTCAGCTATTGGACATATATGTCAAACGAAGAAGGTACTTGGTCAAGGGTTATGTAAAGTTTATATCTGTTATGCTTAACTTCATGAATTTCCCCGGTGGTTTTATGCAATGGATATGGATCTTTCTTATCTTTCAGGACTGGGAAGGTGGAAGGTTATTTTCATTACAAGAACAAATTGATCATAAGGTAACTGTATGTTTTTCTCGGTCGATTTGATTGAATTGTTTATGACTTAGGGAGGCAACTTGTGAGATCCCACATTGGTTGGGGAGGGGAACGAAGTTTTCTTTATAAGGGTGTGGAAACCTTTGCCTAGCATATGTGTTTTAAAACCTTGAGGGGAAACCAGAAAGGGAAAGCCCAAAGAGGACAATATCTGCTAGCGGTGGGCTTGGACTGTTACAAATGGTATCAAAGCTAGATACCGGACGATGTGCCAATGAGGAGGTTGAACCCCGAAGGGGCACGGACACTAGGCGGTGTGCCAGCATGGACGATGGGTCCTGAAGGGGGATGGATTGTGAGATCCCACATTGGTTGGAGAGGGGAATGAAGCATTCTTTGTAAGGGTGTAGAAATCTCTCCCTAGCAGACACGTTTTAAAACCTCGAGGGGAAGCCCGAAAGAGAAAATCTAAAGAGGACAATATCTTCCAGTAGTGGTCTTAGGCGGTTATACAACTAGCCCATGAAAAATTTAAAGTTCCTTTATAAGCTAGCTAGTGACTTCATTTTGCATGTCCAAGATAGCTTTGTAATTGGAGATAGCATTGCAGGCCCTTTCCCTTTCATATTTCAAGGGTTGATATCAGAATTCTGAATGGGGCTGAACTATATTCATCTTTCTTTTGCAGATTGAATTTGTTAAACAAGAGCTACAGAACAAAGAAATTCCATTACTACTGGTATTTACACAATTGATGCTATATTTTCACAATAGTCATAAAAGAATTGCACGGTGCATCGTTTCGATATTATCCGTGTACATGTCTTATTCTTTGCCCATACACAATAGTTGCTTGTTGTGCTGCTTGTACTCCTCTTGTTACTTGTCTAATAACAAGACAGCCCACATGATTGTTTGTACTTGTACATTATATTTTCCGTGGTAACTGACTCGTTAGTTATCTTTAAAGGTTGGGCATTCTATTGGATCGCCTATCTCCATTGAATTGTGCCGTTGGTTCCCGGATCGGGTATCGCCTTGAACTCTTTGTGCATCTTATGTGTTCCTAACTTTCAAGACATGGTTCAATGGAAATCTTCTTGATCCTATAGGTTGTGTATTGCATTGGATTGTATCCATTTATGATGGTGAACAGACAATCACGTTGGCAACTTCTTATCGAAAAACTCGCAGGGTACGTGCGTTATTTTGTCTGATAACATGAATTCTACCTTCCCGCATCTACATTTTAGATACTCTATTTAGTTGGTAGCAAGTGAGACATATAGATGATAGTTAATGAACTTTGAGTTCGAGAGTTCGAATCTCGAAAGTTTTACTTTCTCAAACAGCTATACACCGCACCGCGTGACATGGCTTTGTTGGCTTGACCACAAACTTTTGTAATTTGGAAATTTTCGGATTCTGTTGGTCGTGTAGGGGAGTATCTGCGTTGTCCAGTGCGTGTGCACTCGGGTTGAGCCAATTTGAAGTCACTTAGAAGTTAAGACTAATGGATAAGTGCTTGGTTGTTAACCAGTTAAGGGTGTTAAATATTGGTTCATATGACCTTAATAGTTAATAAAACTGGACCATGTAGAAGTCCTTCCATTCATTGCCCACAAACTGGTCCTTAATCCTTTAAGCATCCATTAAAGCTAACCTATGAGGTCATTATATGTTTTGGTATTGCAAGTTTTGTCTCTCTGAGCTCTGCTGGGTTGAAAATTTTGTGCAGATCCCCACTGCTATCAGCTTTGTTTTCTTCTTTTGCCGCGCTGTTGGGCGTATTACCTGTTCGAGCTTCAAGTTTTGTTGTGAAAAAAACTATTGGGAAGTCATGGTCTAGAACTGCTAGTGAGGCTGCTTGCTCTCACTTGCTAAAGGTGAAATGAAATCTAATATATTATCCTTAGTATGTGCTTTTTTAGTATCCGCTCGAACCTGCTACAAATTTTGGTTCGACGACATGACTGTTCGTCTGATATTTGGAATATTATCTTTGTTTTCGACAGTACCATACGATGCGAAATGTGCTATATATGGCAATGACGGAGTTCGAGAAGGTATTCACTATCAACAATCACTTTCGTTTGGGTCGTATTGGATGCCATGAGATTTGTAAGCATATGATTGTACGAACCATAATTAATCGTAAATCTCATTTGCATAACATTTTTTAATCATTTAGAGAAGCCTAGCTTGTTTTAGTTTTTGTTCTAATCTTTTGCTCTGAGGCGTTGCACTATATGAAATATAGTATAGTTATAACAGCCCAAACCCATCGCTAATAGATATTGTCCGTTGTAGCCCGTAATGTATCGCCATCAGCCTCACAGTTTTTTATCTACTATGGAGAGGGTCTAGTCCTACTCTGACCAGGGCCTCTTGGTAGGTGTGCTAGCAAAGACGTTGGCCCCCCAAGGGGAATGGACTGTGAGATCCCACATCGGTTGGAGAGGGGAACAAAGCATCCCTTATAAGAGTGTGAAAACTTCTCTTTAGCAGACGCGTTTTAAAACCTTGAGGCTAACGGCGATACATAACGGGTCAAATCGGACAATTAGTCCAATCAAAAGATGTAAACAAATGACTAGTCACACAGAACAAAGCCTAATATCAATAATCAATCAAATTTCTACATCTGATTGTTTATGGACAATGTTCAGCTTTCAGAAACACCAGATTGGAAATTTATGAAGAAAGCAAGTCAGAAACTATCATTTTTGTTCTGTAAGGATGATCACTGGGCTCCTATGCATGTGTTTGAGGAGGTAAGATATGAACTGTTCTTCCATTTTCATTTTGTCAACCAATTCTGCTGGGCGAATGTAGTTTCTTCAAATACTTTTGGTGAAGAGTTTATAGTTCATATCGTTTATGACGATCGGAATCGGGAAGTACAACGATAAGCCATTTGAGAAAAAGGAAGGGGCTAAAGGGCTCAAAGTTATGAGACTGCCATGGTGATTTCAAGTTCTAGTTTGATAGTTTATGTTGTCTTTGGTCCACAGATTTGCCAGCAGGTCCCTGAAATTGATGCATCAGTTGAAAGGGAGGAGGGCCATAAACATGCATTTTGCTGCTCTGAAGCTGGCTCACTTCATATTGCTCAATATGTGGCAAGCTTGATCAAAAATCATCTCTCAGATTAA

mRNA sequence

ATGGATCAAGAAGATCTGCAATCGTTTAGTAGAAGGCGTGTGGAGTTTCGTTTATGCAATGTATCAGGCTACACCAATGAATTGCTGGAGATTCACTCGGATAATCCATCATTGCACGTATTGTTCATACCTGGAAATCCAGGGATTATTTCGTTTTATAAACATTTTGTGGAATCATTGTATCAGTTGCTTGGAGGCCGTGTATCTATAACAGCTATTGGACATATATGTCAAACGAAGAAGGACTGGGAAGGTGGAAGGTTATTTTCATTACAAGAACAAATTGATCATAAGATTGAATTTGTTAAACAAGAGCTACAGAACAAAGAAATTCCATTACTACTGGTTGGGCATTCTATTGGATCGCCTATCTCCATTGAATTGTGCCGTTGGTTCCCGGATCGGGTTGTGTATTGCATTGGATTGTATCCATTTATGATGGTGAACAGACAATCACGTTGGCAACTTCTTATCGAAAAACTCGCAGGATCCCCACTGCTATCAGCTTTGTTTTCTTCTTTTGCCGCGCTGTTGGGCGTATTACCTGTTCGAGCTTCAAGTTTTGTTGTGAAAAAAACTATTGGGAAGTCATGGTCTAGAACTGCTAGTGAGGCTGCTTGCTCTCACTTGCTAAAGTACCATACGATGCGAAATGTGCTATATATGGCAATGACGGAGTTCGAGAAGCTTTCAGAAACACCAGATTGGAAATTTATGAAGAAAGCAAGTCAGAAACTATCATTTTTGTTCTGTAAGGATGATCACTGGGCTCCTATGCATGTGTTTGAGGAGATTTGCCAGCAGGTCCCTGAAATTGATGCATCAGTTGAAAGGGAGGAGGGCCATAAACATGCATTTTGCTGCTCTGAAGCTGGCTCACTTCATATTGCTCAATATGTGGCAAGCTTGATCAAAAATCATCTCTCAGATTAA

Coding sequence (CDS)

ATGGATCAAGAAGATCTGCAATCGTTTAGTAGAAGGCGTGTGGAGTTTCGTTTATGCAATGTATCAGGCTACACCAATGAATTGCTGGAGATTCACTCGGATAATCCATCATTGCACGTATTGTTCATACCTGGAAATCCAGGGATTATTTCGTTTTATAAACATTTTGTGGAATCATTGTATCAGTTGCTTGGAGGCCGTGTATCTATAACAGCTATTGGACATATATGTCAAACGAAGAAGGACTGGGAAGGTGGAAGGTTATTTTCATTACAAGAACAAATTGATCATAAGATTGAATTTGTTAAACAAGAGCTACAGAACAAAGAAATTCCATTACTACTGGTTGGGCATTCTATTGGATCGCCTATCTCCATTGAATTGTGCCGTTGGTTCCCGGATCGGGTTGTGTATTGCATTGGATTGTATCCATTTATGATGGTGAACAGACAATCACGTTGGCAACTTCTTATCGAAAAACTCGCAGGATCCCCACTGCTATCAGCTTTGTTTTCTTCTTTTGCCGCGCTGTTGGGCGTATTACCTGTTCGAGCTTCAAGTTTTGTTGTGAAAAAAACTATTGGGAAGTCATGGTCTAGAACTGCTAGTGAGGCTGCTTGCTCTCACTTGCTAAAGTACCATACGATGCGAAATGTGCTATATATGGCAATGACGGAGTTCGAGAAGCTTTCAGAAACACCAGATTGGAAATTTATGAAGAAAGCAAGTCAGAAACTATCATTTTTGTTCTGTAAGGATGATCACTGGGCTCCTATGCATGTGTTTGAGGAGATTTGCCAGCAGGTCCCTGAAATTGATGCATCAGTTGAAAGGGAGGAGGGCCATAAACATGCATTTTGCTGCTCTGAAGCTGGCTCACTTCATATTGCTCAATATGTGGCAAGCTTGATCAAAAATCATCTCTCAGATTAA

Protein sequence

MDQEDLQSFSRRRVEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYKHFVESLYQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSIGSPISIELCRWFPDRVVYCIGLYPFMMVNRQSRWQLLIEKLAGSPLLSALFSSFAALLGVLPVRASSFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMAMTEFEKLSETPDWKFMKKASQKLSFLFCKDDHWAPMHVFEEICQQVPEIDASVEREEGHKHAFCCSEAGSLHIAQYVASLIKNHLSD
Homology
BLAST of Csor.00g143240 vs. ExPASy Swiss-Prot
Match: Q5HZX7 (Lipid droplet-associated hydrolase OS=Rattus norvegicus OX=10116 GN=Ldah PE=2 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 2.7e-20
Identity = 87/321 (27.10%), Postives = 145/321 (45.17%), Query Frame = 0

Query: 14  VEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYKHFVESLYQLLGGRVSITAI 73
           VE ++     +TN   +     P   +  IPGNPG+  FY  F ++LY L+ G   +  I
Sbjct: 21  VETQIIKCGPWTNLFDKQGVSKPKHLIFVIPGNPGLSPFYVPFAKALYSLVKGHFPVWII 80

Query: 74  GH--ICQTKKD------------WEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHS 133
            H   C T KD             E   ++ L  QI+HKI F++  +  K++ L+ +GHS
Sbjct: 81  SHAGFCLTPKDKKILTAPQEPNAQEIEDIYGLNGQIEHKIAFLRAHVP-KDVKLIFIGHS 140

Query: 134 IGSPISIELCRWFPD-RVVYCIGLYPFMMVNRQSRWQLLIEKLAGSP-----------LL 193
           +GS I++ +    P+  VV+   L+P             IE+++ SP             
Sbjct: 141 VGSYIALHVMNRAPELPVVHTFLLFP------------TIERMSESPNGKFATPFLCRFR 200

Query: 194 SALFSSFAALLGVLPVRASSFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMAMTEF 253
             L+++   +    P    SF+V+K + K   +  SE   + LL+   + N  Y+   E 
Sbjct: 201 YMLYAASYLIFKPCPEMIKSFIVQKVLEKMSFK--SELRLTDLLQPFCLANAAYLGGQEM 260

Query: 254 EKLSETPDWKFMKKASQKLSFLFCKDDHWAPMHVFEEICQQVPEIDASVEREEGHKHAFC 309
             + +  D   +K+   KL+F + K D W P++ +E++ +  PE +  +  E+G  HAF 
Sbjct: 261 IHVVKRDD-GIIKELLPKLTFYYGKTDGWCPVNYYEDMKRDFPEANLHL-CEKGIPHAFV 320

BLAST of Csor.00g143240 vs. ExPASy Swiss-Prot
Match: Q54LL8 (Lipid droplet-associated hydrolase OS=Dictyostelium discoideum OX=44689 GN=DDB_G0286581 PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.1e-18
Identity = 85/300 (28.33%), Postives = 141/300 (47.00%), Query Frame = 0

Query: 27  ELLEIHSDNPS-LHVLFIPGNPGIISFYKHFVESLYQLLGGRVSITAIGHICQTKKDWEG 86
           E++   S  PS + ++ I GNPGI SFY+ FV+ L      +  I  +GHI    K    
Sbjct: 21  EIIYTKSQTPSNIKIIVIAGNPGIESFYQEFVKVLNLSFNSKYDIYGVGHIGHCGK--IE 80

Query: 87  GRLFSLQEQIDHKIEFVKQELQN-------KEIPLLLVGHSIGSPISIELCRWFPDRVVY 146
            + FS++EQI HK  F++  L+N       K+I  +L+GHS+GS IS+++   F ++  +
Sbjct: 81  NKTFSVEEQIKHKELFLEYLLKNKYGDKDRKDIKFILIGHSVGSYISLKVVSRFSEKFEF 140

Query: 147 CIGLYPFMMVNRQSRWQLLIEKLAGSPLLSALF------SSFAALLGVLPVRASSFVVKK 206
                   +VN    ++ L + L  SP +  +       +  +  L  +P    S V+K 
Sbjct: 141 ------LSVVNLFPTFKNLYDGL--SPFIKMVVMRESTRNGLSTFLHYIPSIVVSNVLKW 200

Query: 207 TIGKSWSRTASEAACSHLLKYHTMRNVLYMAMTEFEKLSETPD--WKFMKKASQKLSFLF 266
            +    SR A ++     + Y++  N+LYMA TE E + E  D           +L F++
Sbjct: 201 ILPSDESRIAVQSK----INYYSALNILYMAYTETEDIKEIDDECHSVFNSRLNQLLFIY 260

Query: 267 CKDDHWAPMHVFEEICQQVPEIDASVEREEGH-KHAFCCSEAGSLHIAQYVASLIKNHLS 310
            + D + P   ++E+ Q  P    ++E    +  HAF       LH +Q VA  +   LS
Sbjct: 261 GQTDSYTPKSFYDEMKQLYPA--GNIEYSSSYVPHAFV------LHHSQEVALRVSEWLS 298

BLAST of Csor.00g143240 vs. ExPASy Swiss-Prot
Match: Q5F477 (Lipid droplet-associated hydrolase OS=Gallus gallus OX=9031 GN=LDAH PE=2 SV=2)

HSP 1 Score: 95.1 bits (235), Expect = 1.5e-18
Identity = 82/290 (28.28%), Postives = 142/290 (48.97%), Query Frame = 0

Query: 36  PSLHVLFIPGNPGIISFYKHFVESLYQLLGGRVSITAIGHICQTK-------------KD 95
           P L  + IPGNPG+  +Y+ F+++LY  L  +  +  + H    K             K+
Sbjct: 43  PRLLFMIIPGNPGLAGYYRTFIKALYCGLNQQYPVWVVSHAGHCKPPSGMEMIEDTDIKE 102

Query: 96  WEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSIGSPISIELCRWFPD-RVVYCIG 155
            E   +F L  Q++HK+ F+K+ + +K+I L+L+ HSIG  I++E+ +   + +V+  + 
Sbjct: 103 LED--VFGLNGQVEHKLNFLKKNV-SKDIKLVLIAHSIGCYITLEMMKRASELQVLRSVL 162

Query: 156 LYPFMMVNRQSRWQLLIEKLAGSPLLSALFSSFAALLGVLPVRASSFVVKKTI-GKSWSR 215
           L+P +    QS    L+  L    L  AL+     LL  LP    + +V+  + G     
Sbjct: 163 LFPTIERMAQSPQGKLMTPLL-CKLRYALYMP-VYLLSFLPEGVKASLVRFALRGMKTCD 222

Query: 216 TASEAACSHLLKYHTMRNVLYMAMTEFEKLSETPDWKFMKKASQKLSFLFCKDDHWAPMH 275
            +S     +L     + N+LYMA  E  K+ E  D   +K+  +KL F +   D W P +
Sbjct: 223 ESSITTSVNLFSVDCIANILYMASQEMMKVVER-DSTTIKQNLKKLIFYYGTGDSWCPQN 282

Query: 276 VFEEICQQVPEIDASVEREEGHKHAFCCSEAGSLHIAQYVASLIKNHLSD 311
            ++EI    P+ D  +  E+G +HAF       L  ++ +A++I + L D
Sbjct: 283 YYDEIKMDFPDGDIRL-CEKGLRHAFV------LDASKEMAAMITDWLRD 319

BLAST of Csor.00g143240 vs. ExPASy Swiss-Prot
Match: Q9H6V9 (Lipid droplet-associated hydrolase OS=Homo sapiens OX=9606 GN=LDAH PE=1 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 2.3e-16
Identity = 77/293 (26.28%), Postives = 133/293 (45.39%), Query Frame = 0

Query: 36  PSLHVLFIPGNPGIISFYKHFVESLYQLLGGRVSITAIGH-----------ICQTKKD-- 95
           P L +  IPGNPG  +FY  F ++LY L   R  +  I H           I  T +D  
Sbjct: 43  PKLLIFIIPGNPGFSAFYVPFAKALYSLTNRRFPVWTISHAGHALAPKDKKILTTSEDSN 102

Query: 96  -WEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSIGSPISIELCRWFPDRVVYCIG 155
             E   ++ L  QI+HK+ F++  +  K++ L+L+GHSIGS  ++++ +  P+  V    
Sbjct: 103 AQEIKDIYGLNGQIEHKLAFLRTHVP-KDMKLVLIGHSIGSYFTLQMLKRVPELPV---- 162

Query: 156 LYPFMMVNRQSRWQLLIEKLAGSPLLS----ALFSSFAALLGVLPVRASSFVVKKTIGKS 215
           +  F++     R          +PLL      L+ +   LL   P    S ++++  G  
Sbjct: 163 IRAFLLFPTIERMSESPNGRIATPLLCWFRYVLYVTGYLLLKPCPETIKSLLIRR--GLQ 222

Query: 216 WSRTASEAACSHLLKYHTMRNVLYMAMTEFEKLSETPDWKFMKKASQKLSFLFCKDDHWA 275
                +E +  ++L+   + N  Y+   E  ++ +  D + +K+   KL+F +   D W 
Sbjct: 223 VMNLENEFSPLNILEPFCLANAAYLGGQEMMEVVKRDD-ETIKEHLCKLTFYYGTIDPWC 282

Query: 276 PMHVFEEICQQVPEIDASVEREEGHKHAFCCSEAGSLHIAQYVASLIKNHLSD 311
           P   +E+I +  PE D  +  E+   HAF        H  Q +A +I + L D
Sbjct: 283 PKEYYEDIKKDFPEGDIRL-CEKNIPHAFI------THFNQEMADMIADSLKD 320

BLAST of Csor.00g143240 vs. ExPASy Swiss-Prot
Match: Q5R7E8 (Lipid droplet-associated hydrolase OS=Pongo abelii OX=9601 GN=LDAH PE=2 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 4.4e-15
Identity = 73/293 (24.91%), Postives = 134/293 (45.73%), Query Frame = 0

Query: 36  PSLHVLFIPGNPGIISFYKHFVESLYQLLGGRVSITAIGH-----------ICQTKKD-- 95
           P L +  IPGNPG  +FY  F ++LY L      +  I H           I  T +D  
Sbjct: 43  PKLLIFIIPGNPGFSAFYVPFAKALYSLTNRCFPVWTISHAGHALAPKDKKILTTSEDSN 102

Query: 96  -WEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSIGSPISIELCRWFPDRVVYCIG 155
             E   ++ L  QI+HK+ F++  +  K++ L+L+GHS+GS  ++++ +  P+  V    
Sbjct: 103 AQEIKDIYGLNGQIEHKLAFLRTHVP-KDMKLVLIGHSVGSYFTLQMLKRVPELPV---- 162

Query: 156 LYPFMMVNRQSRWQLLIEKLAGSPLLS----ALFSSFAALLGVLPVRASSFVVKKTIGKS 215
           +  F++     R          +PLL      L+ +   LL   P +  S ++++  G  
Sbjct: 163 IRAFLLFPTIERMSESPNGRIATPLLCWSRYVLYVTGYLLLKPCPEKIKSLLIRR--GLQ 222

Query: 216 WSRTASEAACSHLLKYHTMRNVLYMAMTEFEKLSETPDWKFMKKASQKLSFLFCKDDHWA 275
                +E +  ++L+   + N  ++   E  ++ +  D + +++   KL+F +   D W 
Sbjct: 223 VMNLENEFSPLNILEPFCLANAAHLGGQEMMEVVKRDD-ETIREHLCKLTFYYGTIDPWC 282

Query: 276 PMHVFEEICQQVPEIDASVEREEGHKHAFCCSEAGSLHIAQYVASLIKNHLSD 311
           P   +E+I +  PE D  +  E+   HAF       +H  Q +A +I + L D
Sbjct: 283 PKEYYEDIKKDFPEGDIRL-CEKNIPHAFI------IHFNQEMADMIADSLKD 320

BLAST of Csor.00g143240 vs. NCBI nr
Match: KAG6604325.1 (Lipid droplet-associated hydrolase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 635 bits (1639), Expect = 4.44e-230
Identity = 310/310 (100.00%), Postives = 310/310 (100.00%), Query Frame = 0

Query: 1   MDQEDLQSFSRRRVEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYKHFVESL 60
           MDQEDLQSFSRRRVEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYKHFVESL
Sbjct: 1   MDQEDLQSFSRRRVEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYKHFVESL 60

Query: 61  YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSI 120
           YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSI
Sbjct: 61  YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSI 120

Query: 121 GSPISIELCRWFPDRVVYCIGLYPFMMVNRQSRWQLLIEKLAGSPLLSALFSSFAALLGV 180
           GSPISIELCRWFPDRVVYCIGLYPFMMVNRQSRWQLLIEKLAGSPLLSALFSSFAALLGV
Sbjct: 121 GSPISIELCRWFPDRVVYCIGLYPFMMVNRQSRWQLLIEKLAGSPLLSALFSSFAALLGV 180

Query: 181 LPVRASSFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMAMTEFEKLSETPDWKFMK 240
           LPVRASSFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMAMTEFEKLSETPDWKFMK
Sbjct: 181 LPVRASSFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMAMTEFEKLSETPDWKFMK 240

Query: 241 KASQKLSFLFCKDDHWAPMHVFEEICQQVPEIDASVEREEGHKHAFCCSEAGSLHIAQYV 300
           KASQKLSFLFCKDDHWAPMHVFEEICQQVPEIDASVEREEGHKHAFCCSEAGSLHIAQYV
Sbjct: 241 KASQKLSFLFCKDDHWAPMHVFEEICQQVPEIDASVEREEGHKHAFCCSEAGSLHIAQYV 300

Query: 301 ASLIKNHLSD 310
           ASLIKNHLSD
Sbjct: 301 ASLIKNHLSD 310

BLAST of Csor.00g143240 vs. NCBI nr
Match: XP_023543375.1 (lipid droplet-associated hydrolase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 625 bits (1612), Expect = 5.81e-226
Identity = 304/310 (98.06%), Postives = 307/310 (99.03%), Query Frame = 0

Query: 1   MDQEDLQSFSRRRVEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYKHFVESL 60
           MDQEDLQSFSRRRVEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYK FVESL
Sbjct: 1   MDQEDLQSFSRRRVEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYKDFVESL 60

Query: 61  YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSI 120
           YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSI
Sbjct: 61  YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSI 120

Query: 121 GSPISIELCRWFPDRVVYCIGLYPFMMVNRQSRWQLLIEKLAGSPLLSALFSSFAALLGV 180
           GSPISIELCRWFPDRVVYCIGLYPFMMVNRQSRWQLLIEKLAGSPLLS LFSSFAALLGV
Sbjct: 121 GSPISIELCRWFPDRVVYCIGLYPFMMVNRQSRWQLLIEKLAGSPLLSTLFSSFAALLGV 180

Query: 181 LPVRASSFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMAMTEFEKLSETPDWKFMK 240
           LPVRASSFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMAMTEFEKLSETPDWKFMK
Sbjct: 181 LPVRASSFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMAMTEFEKLSETPDWKFMK 240

Query: 241 KASQKLSFLFCKDDHWAPMHVFEEICQQVPEIDASVEREEGHKHAFCCSEAGSLHIAQYV 300
           KAS+KLSFLFCKDDHWAPMHVFEEIC+QVPEID SVEREEGHKHAFCCSEAGSLHIAQYV
Sbjct: 241 KASEKLSFLFCKDDHWAPMHVFEEICKQVPEIDVSVEREEGHKHAFCCSEAGSLHIAQYV 300

Query: 301 ASLIKNHLSD 310
           ASLIKNHL+D
Sbjct: 301 ASLIKNHLAD 310

BLAST of Csor.00g143240 vs. NCBI nr
Match: XP_022979068.1 (lipid droplet-associated hydrolase-like [Cucurbita maxima] >XP_022979069.1 lipid droplet-associated hydrolase-like [Cucurbita maxima] >XP_022979070.1 lipid droplet-associated hydrolase-like [Cucurbita maxima])

HSP 1 Score: 622 bits (1603), Expect = 1.37e-224
Identity = 302/310 (97.42%), Postives = 305/310 (98.39%), Query Frame = 0

Query: 1   MDQEDLQSFSRRRVEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYKHFVESL 60
           MDQEDLQSFSRRRVEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYK FVESL
Sbjct: 1   MDQEDLQSFSRRRVEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYKDFVESL 60

Query: 61  YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSI 120
           YQLLGGRVSITAIGHICQT KDWEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSI
Sbjct: 61  YQLLGGRVSITAIGHICQTNKDWEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSI 120

Query: 121 GSPISIELCRWFPDRVVYCIGLYPFMMVNRQSRWQLLIEKLAGSPLLSALFSSFAALLGV 180
           GSPISIELCRWFPDRVVYCIGLYPFMMVNRQSRWQLLIEKLAGSPLLS LFSSFAALLGV
Sbjct: 121 GSPISIELCRWFPDRVVYCIGLYPFMMVNRQSRWQLLIEKLAGSPLLSTLFSSFAALLGV 180

Query: 181 LPVRASSFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMAMTEFEKLSETPDWKFMK 240
           LPVRASSFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMA TEFEKLSETPDWKFMK
Sbjct: 181 LPVRASSFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMAKTEFEKLSETPDWKFMK 240

Query: 241 KASQKLSFLFCKDDHWAPMHVFEEICQQVPEIDASVEREEGHKHAFCCSEAGSLHIAQYV 300
           KAS+KLSFLFCKDDHWAPMHVFEEIC+QVPE+D SVEREEGHKHAFCCSEAGSLHIAQYV
Sbjct: 241 KASEKLSFLFCKDDHWAPMHVFEEICKQVPEVDVSVEREEGHKHAFCCSEAGSLHIAQYV 300

Query: 301 ASLIKNHLSD 310
           ASLIKNHLSD
Sbjct: 301 ASLIKNHLSD 310

BLAST of Csor.00g143240 vs. NCBI nr
Match: XP_022926102.1 (lipid droplet-associated hydrolase-like [Cucurbita moschata] >XP_022926103.1 lipid droplet-associated hydrolase-like [Cucurbita moschata] >XP_022926104.1 lipid droplet-associated hydrolase-like [Cucurbita moschata])

HSP 1 Score: 621 bits (1602), Expect = 1.94e-224
Identity = 305/310 (98.39%), Postives = 306/310 (98.71%), Query Frame = 0

Query: 1   MDQEDLQSFSRRRVEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYKHFVESL 60
           MDQE LQ FSRRRVEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYK FVESL
Sbjct: 1   MDQEALQLFSRRRVEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYKDFVESL 60

Query: 61  YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSI 120
           YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSI
Sbjct: 61  YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSI 120

Query: 121 GSPISIELCRWFPDRVVYCIGLYPFMMVNRQSRWQLLIEKLAGSPLLSALFSSFAALLGV 180
           GSPISIELCRWF DRVVYCIGLYPFMMVNRQSRWQLLIEKLAGSPLLSALFSSFAALLGV
Sbjct: 121 GSPISIELCRWFLDRVVYCIGLYPFMMVNRQSRWQLLIEKLAGSPLLSALFSSFAALLGV 180

Query: 181 LPVRASSFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMAMTEFEKLSETPDWKFMK 240
           LPVRASSFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMAMTEFEKLSETPDWKFMK
Sbjct: 181 LPVRASSFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMAMTEFEKLSETPDWKFMK 240

Query: 241 KASQKLSFLFCKDDHWAPMHVFEEICQQVPEIDASVEREEGHKHAFCCSEAGSLHIAQYV 300
           KASQKLSFLFCKDDHWAPMHVFEEIC+QVPEIDASVEREEGHKHAFCCSEAGSLHIAQYV
Sbjct: 241 KASQKLSFLFCKDDHWAPMHVFEEICKQVPEIDASVEREEGHKHAFCCSEAGSLHIAQYV 300

Query: 301 ASLIKNHLSD 310
           ASLIKNHLSD
Sbjct: 301 ASLIKNHLSD 310

BLAST of Csor.00g143240 vs. NCBI nr
Match: XP_038881163.1 (lipid droplet-associated hydrolase [Benincasa hispida])

HSP 1 Score: 539 bits (1389), Expect = 1.82e-191
Identity = 268/310 (86.45%), Postives = 282/310 (90.97%), Query Frame = 0

Query: 1   MDQEDLQSFSRRRVEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYKHFVESL 60
           M  + LQSFS+ RVE RLCNVSGYTNELLEIHSD+PSLHVLFIPGNPGIISFYK FVESL
Sbjct: 34  MGHQVLQSFSKSRVELRLCNVSGYTNELLEIHSDHPSLHVLFIPGNPGIISFYKDFVESL 93

Query: 61  YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSI 120
           YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFV+QELQNK+IPLLLVGHS+
Sbjct: 94  YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSV 153

Query: 121 GSPISIELCRWFPDRVVYCIGLYPFMMVNRQSRWQLLIEKLAGSPLLSALFSSFAALLGV 180
           GS ISIEL R F DRVVYCIGL+PFMMVNRQSR Q  IEKLA S LLS LFSSF ALLGV
Sbjct: 154 GSYISIELYRRFQDRVVYCIGLHPFMMVNRQSRQQFFIEKLARSSLLSTLFSSFVALLGV 213

Query: 181 LPVRASSFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMAMTEFEKLSETPDWKFMK 240
           LP+RASSFVV+KT GKSWSRTASEAACSHLLKYH+MRNVLYMAM EFEK SETPDW FMK
Sbjct: 214 LPIRASSFVVRKTFGKSWSRTASEAACSHLLKYHSMRNVLYMAMMEFEKFSETPDWAFMK 273

Query: 241 KASQKLSFLFCKDDHWAPMHVFEEICQQVPEIDASVEREEGHKHAFCCSEAGSLHIAQYV 300
           KASQKLSFLFC DDHWAPMHV+EEI +QVPEID SVERE GH HAFCCSEA SL+IAQYV
Sbjct: 274 KASQKLSFLFCMDDHWAPMHVYEEISKQVPEIDLSVERE-GHSHAFCCSEAASLYIAQYV 333

Query: 301 ASLIKNHLSD 310
           ASL+K HLSD
Sbjct: 334 ASLVKKHLSD 342

BLAST of Csor.00g143240 vs. ExPASy TrEMBL
Match: A0A6J1IV27 (lipid droplet-associated hydrolase-like OS=Cucurbita maxima OX=3661 GN=LOC111478816 PE=3 SV=1)

HSP 1 Score: 622 bits (1603), Expect = 6.62e-225
Identity = 302/310 (97.42%), Postives = 305/310 (98.39%), Query Frame = 0

Query: 1   MDQEDLQSFSRRRVEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYKHFVESL 60
           MDQEDLQSFSRRRVEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYK FVESL
Sbjct: 1   MDQEDLQSFSRRRVEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYKDFVESL 60

Query: 61  YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSI 120
           YQLLGGRVSITAIGHICQT KDWEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSI
Sbjct: 61  YQLLGGRVSITAIGHICQTNKDWEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSI 120

Query: 121 GSPISIELCRWFPDRVVYCIGLYPFMMVNRQSRWQLLIEKLAGSPLLSALFSSFAALLGV 180
           GSPISIELCRWFPDRVVYCIGLYPFMMVNRQSRWQLLIEKLAGSPLLS LFSSFAALLGV
Sbjct: 121 GSPISIELCRWFPDRVVYCIGLYPFMMVNRQSRWQLLIEKLAGSPLLSTLFSSFAALLGV 180

Query: 181 LPVRASSFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMAMTEFEKLSETPDWKFMK 240
           LPVRASSFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMA TEFEKLSETPDWKFMK
Sbjct: 181 LPVRASSFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMAKTEFEKLSETPDWKFMK 240

Query: 241 KASQKLSFLFCKDDHWAPMHVFEEICQQVPEIDASVEREEGHKHAFCCSEAGSLHIAQYV 300
           KAS+KLSFLFCKDDHWAPMHVFEEIC+QVPE+D SVEREEGHKHAFCCSEAGSLHIAQYV
Sbjct: 241 KASEKLSFLFCKDDHWAPMHVFEEICKQVPEVDVSVEREEGHKHAFCCSEAGSLHIAQYV 300

Query: 301 ASLIKNHLSD 310
           ASLIKNHLSD
Sbjct: 301 ASLIKNHLSD 310

BLAST of Csor.00g143240 vs. ExPASy TrEMBL
Match: A0A6J1EDI9 (lipid droplet-associated hydrolase-like OS=Cucurbita moschata OX=3662 GN=LOC111433317 PE=3 SV=1)

HSP 1 Score: 621 bits (1602), Expect = 9.41e-225
Identity = 305/310 (98.39%), Postives = 306/310 (98.71%), Query Frame = 0

Query: 1   MDQEDLQSFSRRRVEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYKHFVESL 60
           MDQE LQ FSRRRVEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYK FVESL
Sbjct: 1   MDQEALQLFSRRRVEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYKDFVESL 60

Query: 61  YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSI 120
           YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSI
Sbjct: 61  YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSI 120

Query: 121 GSPISIELCRWFPDRVVYCIGLYPFMMVNRQSRWQLLIEKLAGSPLLSALFSSFAALLGV 180
           GSPISIELCRWF DRVVYCIGLYPFMMVNRQSRWQLLIEKLAGSPLLSALFSSFAALLGV
Sbjct: 121 GSPISIELCRWFLDRVVYCIGLYPFMMVNRQSRWQLLIEKLAGSPLLSALFSSFAALLGV 180

Query: 181 LPVRASSFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMAMTEFEKLSETPDWKFMK 240
           LPVRASSFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMAMTEFEKLSETPDWKFMK
Sbjct: 181 LPVRASSFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMAMTEFEKLSETPDWKFMK 240

Query: 241 KASQKLSFLFCKDDHWAPMHVFEEICQQVPEIDASVEREEGHKHAFCCSEAGSLHIAQYV 300
           KASQKLSFLFCKDDHWAPMHVFEEIC+QVPEIDASVEREEGHKHAFCCSEAGSLHIAQYV
Sbjct: 241 KASQKLSFLFCKDDHWAPMHVFEEICKQVPEIDASVEREEGHKHAFCCSEAGSLHIAQYV 300

Query: 301 ASLIKNHLSD 310
           ASLIKNHLSD
Sbjct: 301 ASLIKNHLSD 310

BLAST of Csor.00g143240 vs. ExPASy TrEMBL
Match: A0A0A0KFF6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G454400 PE=3 SV=1)

HSP 1 Score: 533 bits (1373), Expect = 2.41e-189
Identity = 260/304 (85.53%), Postives = 281/304 (92.43%), Query Frame = 0

Query: 7   QSFSRRRVEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYKHFVESLYQLLGG 66
           QSFS+RRVEFRLCNVSG+TNELLEIHSD+PSLHVLFIPGNPGIISFYK F+ESLYQLLGG
Sbjct: 40  QSFSKRRVEFRLCNVSGFTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFLESLYQLLGG 99

Query: 67  RVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSIGSPISI 126
            VSITAIGHICQTKKDWEGGRLFSLQEQIDHK+EFV+QELQNK+IPLLLVGHS+GS ISI
Sbjct: 100 HVSITAIGHICQTKKDWEGGRLFSLQEQIDHKVEFVRQELQNKDIPLLLVGHSVGSYISI 159

Query: 127 ELCRWFPDRVVYCIGLYPFMMVNRQSRWQLLIEKLAGSPLLSALFSSFAALLGVLPVRAS 186
           EL R F DR VYCIGL+PFMMVNR+SR Q  IEKL+ SPLLS LFSSFAALLG LP++AS
Sbjct: 160 ELFRRFQDRAVYCIGLHPFMMVNRESRQQFFIEKLSRSPLLSTLFSSFAALLGTLPIQAS 219

Query: 187 SFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMAMTEFEKLSETPDWKFMKKASQKL 246
           SFVVKKTIGKSWSRTASEAACSHLLKYH+MRNVLYMAMTEF+K SETPDW FMKK SQKL
Sbjct: 220 SFVVKKTIGKSWSRTASEAACSHLLKYHSMRNVLYMAMTEFDKFSETPDWAFMKKVSQKL 279

Query: 247 SFLFCKDDHWAPMHVFEEICQQVPEIDASVEREEGHKHAFCCSEAGSLHIAQYVASLIKN 306
           SFLFC DDHWAPMHV+EEI +QVPEID SVERE G+ HAFCCSEA S++IAQYVASL+K 
Sbjct: 280 SFLFCMDDHWAPMHVYEEISKQVPEIDLSVERE-GYSHAFCCSEAASMYIAQYVASLVKK 339

Query: 307 HLSD 310
           HLSD
Sbjct: 340 HLSD 342

BLAST of Csor.00g143240 vs. ExPASy TrEMBL
Match: A0A5A7SKD4 (Lipid droplet-associated hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G001690 PE=3 SV=1)

HSP 1 Score: 532 bits (1370), Expect = 6.90e-189
Identity = 259/310 (83.55%), Postives = 282/310 (90.97%), Query Frame = 0

Query: 1   MDQEDLQSFSRRRVEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYKHFVESL 60
           M  + LQSFS+RRVEFRLCNVSG+TNELLEIHSD+PSLHVLFIPGNPGIISFYK FVESL
Sbjct: 34  MGHQVLQSFSKRRVEFRLCNVSGFTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFVESL 93

Query: 61  YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSI 120
           YQLLGG VSITAIGHICQTKKDWEGGRLFSLQEQIDHK+EFV+QELQNK+IPL+LVGHS+
Sbjct: 94  YQLLGGHVSITAIGHICQTKKDWEGGRLFSLQEQIDHKVEFVRQELQNKDIPLILVGHSV 153

Query: 121 GSPISIELCRWFPDRVVYCIGLYPFMMVNRQSRWQLLIEKLAGSPLLSALFSSFAALLGV 180
           GS ISIEL R F DR VYCIGL+PFMMVN++SR Q  IEKLA SPLLS  FSSF ALLG+
Sbjct: 154 GSYISIELFRRFQDRAVYCIGLHPFMMVNKESRQQFFIEKLARSPLLSTFFSSFVALLGI 213

Query: 181 LPVRASSFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMAMTEFEKLSETPDWKFMK 240
           LP++ASSFVV+KTIGKSWSRTA EAACSHLLKYH+MRNVLYMAMTEFEK SETPDW FMK
Sbjct: 214 LPIQASSFVVRKTIGKSWSRTACEAACSHLLKYHSMRNVLYMAMTEFEKFSETPDWAFMK 273

Query: 241 KASQKLSFLFCKDDHWAPMHVFEEICQQVPEIDASVEREEGHKHAFCCSEAGSLHIAQYV 300
           K SQKLSFLFC DDHWAPMHV+EEI +QVPEID SVERE G+ HAFCCSEA S++IAQYV
Sbjct: 274 KVSQKLSFLFCMDDHWAPMHVYEEIFKQVPEIDLSVERE-GYSHAFCCSEAASIYIAQYV 333

Query: 301 ASLIKNHLSD 310
           ASL+K HLSD
Sbjct: 334 ASLVKKHLSD 342

BLAST of Csor.00g143240 vs. ExPASy TrEMBL
Match: A0A1S3CHU9 (LOW QUALITY PROTEIN: lipid droplet-associated hydrolase OS=Cucumis melo OX=3656 GN=LOC103500925 PE=3 SV=1)

HSP 1 Score: 531 bits (1369), Expect = 9.80e-189
Identity = 259/310 (83.55%), Postives = 282/310 (90.97%), Query Frame = 0

Query: 1   MDQEDLQSFSRRRVEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYKHFVESL 60
           M  + LQSFS+RRVEFRLCNVSG+TNELLEIHSD+PSLHVLFIPGNPGIISFYK FVESL
Sbjct: 34  MGHQVLQSFSKRRVEFRLCNVSGFTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFVESL 93

Query: 61  YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSI 120
           YQLLGG VSITAIGHICQTKKDWEGGRLFSLQEQIDHK+EFV+QELQNK+IPL+LVGHS+
Sbjct: 94  YQLLGGHVSITAIGHICQTKKDWEGGRLFSLQEQIDHKVEFVRQELQNKDIPLILVGHSV 153

Query: 121 GSPISIELCRWFPDRVVYCIGLYPFMMVNRQSRWQLLIEKLAGSPLLSALFSSFAALLGV 180
           GS ISIEL R F DR VYCIGL+PFMMVN++SR Q  IEKLA SPLLS  FSSF ALLG+
Sbjct: 154 GSYISIELFRRFQDRAVYCIGLHPFMMVNKESRQQFFIEKLARSPLLSXFFSSFVALLGI 213

Query: 181 LPVRASSFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMAMTEFEKLSETPDWKFMK 240
           LP++ASSFVV+KTIGKSWSRTA EAACSHLLKYH+MRNVLYMAMTEFEK SETPDW FMK
Sbjct: 214 LPIQASSFVVRKTIGKSWSRTACEAACSHLLKYHSMRNVLYMAMTEFEKFSETPDWAFMK 273

Query: 241 KASQKLSFLFCKDDHWAPMHVFEEICQQVPEIDASVEREEGHKHAFCCSEAGSLHIAQYV 300
           K SQKLSFLFC DDHWAPMHV+EEI +QVPEID SVERE G+ HAFCCSEA S++IAQYV
Sbjct: 274 KVSQKLSFLFCMDDHWAPMHVYEEIFKQVPEIDLSVERE-GYSHAFCCSEAASIYIAQYV 333

Query: 301 ASLIKNHLSD 310
           ASL+K HLSD
Sbjct: 334 ASLVKKHLSD 342

BLAST of Csor.00g143240 vs. TAIR 10
Match: AT3G11620.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 372.1 bits (954), Expect = 4.4e-103
Identity = 177/307 (57.65%), Postives = 230/307 (74.92%), Query Frame = 0

Query: 3   QEDLQSFSRRRVEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYKHFVESLYQ 62
           Q  L + ++R V+ RLC VSG   E++EI ++NP+ HVLFIPGNPG++SFYK F+ESLY+
Sbjct: 4   QNKLMNETKRHVKSRLCRVSGSMTEMMEIQAENPTFHVLFIPGNPGVVSFYKDFLESLYE 63

Query: 63  LLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSIGS 122
            LGG  S+ AIG I  T KDWE GRLFS QEQIDHKI+F++QEL++ ++P++LVGHSIGS
Sbjct: 64  FLGGNASVIAIGQISHTSKDWESGRLFSFQEQIDHKIDFIRQELESVKVPIILVGHSIGS 123

Query: 123 PISIELCRWFPDRVVYCIGLYPFMMVNRQSRWQLLIEKLAGSPLLSALFSSFAALLGVLP 182
            IS+EL + F D+VVYCIGLYPF+ +N+QS  Q LI KLA S +LSA  S   A L +LP
Sbjct: 124 YISLELLKKFSDKVVYCIGLYPFLTLNQQSTKQSLIGKLAASSVLSATASFLIASLRLLP 183

Query: 183 VRASSFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMAMTEFEKLSETPDWKFMKKA 242
           + A+  +V K+IG SWS TA +A C+HL +YHTMRNVL+MA +EF +L+  PDW FM++ 
Sbjct: 184 MSAARLLVSKSIGASWSDTAVQATCTHLRQYHTMRNVLFMAKSEFRELAAEPDWDFMREN 243

Query: 243 SQKLSFLFCKDDHWAPMHVFEEICQQVPEIDASVEREEGHKHAFCCSEAGSLHIAQYVAS 302
             KL+FLF  DDHW P+ +FEEI +Q P    S+ER EGH H FCC+ AGS  +AQ+VA+
Sbjct: 244 QSKLAFLFGIDDHWGPLQLFEEISKQAPHTSLSIER-EGHTHGFCCTVAGSAWVAQHVAT 303

Query: 303 LIKNHLS 310
           LIKN  S
Sbjct: 304 LIKNRFS 309

BLAST of Csor.00g143240 vs. TAIR 10
Match: AT3G11620.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 372.1 bits (954), Expect = 4.4e-103
Identity = 177/307 (57.65%), Postives = 230/307 (74.92%), Query Frame = 0

Query: 3   QEDLQSFSRRRVEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYKHFVESLYQ 62
           Q  L + ++R V+ RLC VSG   E++EI ++NP+ HVLFIPGNPG++SFYK F+ESLY+
Sbjct: 4   QNKLMNETKRHVKSRLCRVSGSMTEMMEIQAENPTFHVLFIPGNPGVVSFYKDFLESLYE 63

Query: 63  LLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSIGS 122
            LGG  S+ AIG I  T KDWE GRLFS QEQIDHKI+F++QEL++ ++P++LVGHSIGS
Sbjct: 64  FLGGNASVIAIGQISHTSKDWESGRLFSFQEQIDHKIDFIRQELESVKVPIILVGHSIGS 123

Query: 123 PISIELCRWFPDRVVYCIGLYPFMMVNRQSRWQLLIEKLAGSPLLSALFSSFAALLGVLP 182
            IS+EL + F D+VVYCIGLYPF+ +N+QS  Q LI KLA S +LSA  S   A L +LP
Sbjct: 124 YISLELLKKFSDKVVYCIGLYPFLTLNQQSTKQSLIGKLAASSVLSATASFLIASLRLLP 183

Query: 183 VRASSFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMAMTEFEKLSETPDWKFMKKA 242
           + A+  +V K+IG SWS TA +A C+HL +YHTMRNVL+MA +EF +L+  PDW FM++ 
Sbjct: 184 MSAARLLVSKSIGASWSDTAVQATCTHLRQYHTMRNVLFMAKSEFRELAAEPDWDFMREN 243

Query: 243 SQKLSFLFCKDDHWAPMHVFEEICQQVPEIDASVEREEGHKHAFCCSEAGSLHIAQYVAS 302
             KL+FLF  DDHW P+ +FEEI +Q P    S+ER EGH H FCC+ AGS  +AQ+VA+
Sbjct: 244 QSKLAFLFGIDDHWGPLQLFEEISKQAPHTSLSIER-EGHTHGFCCTVAGSAWVAQHVAT 303

Query: 303 LIKNHLS 310
           LIKN  S
Sbjct: 304 LIKNRFS 309

BLAST of Csor.00g143240 vs. TAIR 10
Match: AT3G11620.3 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 279.6 bits (714), Expect = 3.0e-75
Identity = 133/228 (58.33%), Postives = 175/228 (76.75%), Query Frame = 0

Query: 3   QEDLQSFSRRRVEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYKHFVESLYQ 62
           Q  L + ++R V+ RLC VSG   E++EI ++NP+ HVLFIPGNPG++SFYK F+ESLY+
Sbjct: 4   QNKLMNETKRHVKSRLCRVSGSMTEMMEIQAENPTFHVLFIPGNPGVVSFYKDFLESLYE 63

Query: 63  LLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSIGS 122
            LGG  S+ AIG I  T KDWE GRLFS QEQIDHKI+F++QEL++ ++P++LVGHSIGS
Sbjct: 64  FLGGNASVIAIGQISHTSKDWESGRLFSFQEQIDHKIDFIRQELESVKVPIILVGHSIGS 123

Query: 123 PISIELCRWFPDRVVYCIGLYPFMMVNRQSRWQLLIEKLAGSPLLSALFSSFAALLGVLP 182
            IS+EL + F D+VVYCIGLYPF+ +N+QS  Q LI KLA S +LSA  S   A L +LP
Sbjct: 124 YISLELLKKFSDKVVYCIGLYPFLTLNQQSTKQSLIGKLAASSVLSATASFLIASLRLLP 183

Query: 183 VRASSFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMAMTEFEKL 231
           + A+  +V K+IG SWS TA +A C+HL +YHTMRNVL+MA +EF ++
Sbjct: 184 MSAARLLVSKSIGASWSDTAVQATCTHLRQYHTMRNVLFMAKSEFREV 231

BLAST of Csor.00g143240 vs. TAIR 10
Match: AT3G11620.4 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 279.6 bits (714), Expect = 3.0e-75
Identity = 133/228 (58.33%), Postives = 175/228 (76.75%), Query Frame = 0

Query: 3   QEDLQSFSRRRVEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYKHFVESLYQ 62
           Q  L + ++R V+ RLC VSG   E++EI ++NP+ HVLFIPGNPG++SFYK F+ESLY+
Sbjct: 4   QNKLMNETKRHVKSRLCRVSGSMTEMMEIQAENPTFHVLFIPGNPGVVSFYKDFLESLYE 63

Query: 63  LLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSIGS 122
            LGG  S+ AIG I  T KDWE GRLFS QEQIDHKI+F++QEL++ ++P++LVGHSIGS
Sbjct: 64  FLGGNASVIAIGQISHTSKDWESGRLFSFQEQIDHKIDFIRQELESVKVPIILVGHSIGS 123

Query: 123 PISIELCRWFPDRVVYCIGLYPFMMVNRQSRWQLLIEKLAGSPLLSALFSSFAALLGVLP 182
            IS+EL + F D+VVYCIGLYPF+ +N+QS  Q LI KLA S +LSA  S   A L +LP
Sbjct: 124 YISLELLKKFSDKVVYCIGLYPFLTLNQQSTKQSLIGKLAASSVLSATASFLIASLRLLP 183

Query: 183 VRASSFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMAMTEFEKL 231
           + A+  +V K+IG SWS TA +A C+HL +YHTMRNVL+MA +EF ++
Sbjct: 184 MSAARLLVSKSIGASWSDTAVQATCTHLRQYHTMRNVLFMAKSEFREV 231

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5HZX72.7e-2027.10Lipid droplet-associated hydrolase OS=Rattus norvegicus OX=10116 GN=Ldah PE=2 SV... [more]
Q54LL81.1e-1828.33Lipid droplet-associated hydrolase OS=Dictyostelium discoideum OX=44689 GN=DDB_G... [more]
Q5F4771.5e-1828.28Lipid droplet-associated hydrolase OS=Gallus gallus OX=9031 GN=LDAH PE=2 SV=2[more]
Q9H6V92.3e-1626.28Lipid droplet-associated hydrolase OS=Homo sapiens OX=9606 GN=LDAH PE=1 SV=1[more]
Q5R7E84.4e-1524.91Lipid droplet-associated hydrolase OS=Pongo abelii OX=9601 GN=LDAH PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAG6604325.14.44e-230100.00Lipid droplet-associated hydrolase, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_023543375.15.81e-22698.06lipid droplet-associated hydrolase-like [Cucurbita pepo subsp. pepo][more]
XP_022979068.11.37e-22497.42lipid droplet-associated hydrolase-like [Cucurbita maxima] >XP_022979069.1 lipid... [more]
XP_022926102.11.94e-22498.39lipid droplet-associated hydrolase-like [Cucurbita moschata] >XP_022926103.1 lip... [more]
XP_038881163.11.82e-19186.45lipid droplet-associated hydrolase [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1IV276.62e-22597.42lipid droplet-associated hydrolase-like OS=Cucurbita maxima OX=3661 GN=LOC111478... [more]
A0A6J1EDI99.41e-22598.39lipid droplet-associated hydrolase-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A0A0KFF62.41e-18985.53Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G454400 PE=3 SV=1[more]
A0A5A7SKD46.90e-18983.55Lipid droplet-associated hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3CHU99.80e-18983.55LOW QUALITY PROTEIN: lipid droplet-associated hydrolase OS=Cucumis melo OX=3656 ... [more]
Match NameE-valueIdentityDescription
AT3G11620.24.4e-10357.65alpha/beta-Hydrolases superfamily protein [more]
AT3G11620.14.4e-10357.65alpha/beta-Hydrolases superfamily protein [more]
AT3G11620.33.0e-7558.33alpha/beta-Hydrolases superfamily protein [more]
AT3G11620.43.0e-7558.33alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019363Lipid droplet-associated hydrolasePFAMPF10230LIDHydrolasecoord: 38..287
e-value: 3.9E-61
score: 206.9
IPR019363Lipid droplet-associated hydrolasePANTHERPTHR13390LIPASEcoord: 10..308
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 10..300
e-value: 1.2E-13
score: 53.2
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 34..289
NoneNo IPR availablePANTHERPTHR13390:SF2BNAA05G27450D PROTEINcoord: 10..308

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g143240.m01Csor.00g143240.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019915 lipid storage
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005811 lipid droplet
molecular_function GO:0016298 lipase activity