Csor.00g141020 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g141020
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionCold regulated protein 27
LocationCsor_Chr01: 13631172 .. 13633019 (+)
RNA-Seq ExpressionCsor.00g141020
SyntenyCsor.00g141020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACAACTGTGAAGGTTCTTTTGTCCCCAAACACGGCCTTTCGCTCTCTTCCTAAAGATCTCTCTCCACTGGCCCGCTTCCATTTTTCTTCTCCATCTCTCTCTCTCTCTCGCCATTGCAAATCTGGAGATAATCCTTCATCTTCTTCTGTTTTCATGGAGCTTTTGCCTCCAAATTTAGAGAATCTCAAGCCTAAAGAAACTCGGACCGACTCACTGATCTCTGAATTGGCCATGGACGAGTCAACCGAGGCTCCCCTACAGGACGAATCTTCTAGCCAGGTACTTACCCTACTTTTCTTCTTCACGGTTCCAATTATCTTCGTTTTTGGATGTTTTTGTATTTCGGATTAGGTTTTGCTTATGTGATGTTTCGTATCTTCAATTTCATACTCTGTGCCCGTACTGTTTCTCTATTTTCGTGCTAAAATGAGAACGATATCGATTAAATAATTGAAAGAGAGAGGAAGTGGAAGAAGATAGTACTGGTCTGGCATTGACTCTCGAGACTGGAGAATTTTTATTTTGTATTTTGTTCGGCTCTGTTTAGATCGGTATTGCTGTTTGTCGATGGAATTTATTGGGCTGTGCAAGAGGTTTGGATCTGTGATCAATTATTCTGGAAACTCAGCACGAGCTTTGGTTGTCCGGATTTATTATTTATCATTATAATGACTAATAGTAGCGTTTTTCTGTTATTTATTAAGAGAGACCATGAACTTGCCGCGGGAATTGCTGATAATGCGGTTTCGATTTTAGGGCACTCGTATGACGGAGTCTGTACCTTCTGATTGGACCGATGAGAAGCACCATTTGTTCCTTGAATCCATGGAGGCATCTTTTGTCAGCCAGATGTTTGATTCTGCGCATTCAGGCGGTACCGGCTCCGGCAAGGAAAATTCATCATGGACAAAATTGCACGGCCAAAGCCAGGCCGCTTCTCATGTCCACTCACATGTTGGCCAGGTTCAGTGCCGATTGTTTTTTCTTGATGAGTTTTAACTCGTTTCTGATCATGACTTGTGAGTGCTCTTTTTGTTATTTTGTTGCTTTCATCTACACAGTTTAAGGTTCTTCGACAAGGCAGCTGGAAGAATGTTAATTTTGAACCAACTGAAGCTCGGCCGAATTTATTGAATGATTACCAGGCTCTATCGCGCAATCCATGGATACATCACTTCAGAGCTGCTCGCAGAAACAAAAATGTTGCATGTAAAAGTCCAGCCATCAGTTCAAGAAAAAGGAATTTGTTGCCATTTGGAGGAGATAATAATTCAGGACCTATGCGTGCTTGTAACTCTGATTTGTCTCAGCATTATATATACAACAATAAAGGTAGGTCTCTGGGCCTTTGTGCAGATAAATTATCAATATTGAATTGATAAATTGTGCCTCTCCTTGATTATAATTTAGTTATCACGTTTAAGTTTGCTAGTTAGTAATAATAAATTGGAATATGTTTGATCTGAATCTAGTCTTTACTGCCAGAAAGTTCTGAGTTCAAACAAATGATGCGACTGGAGTGAAATTGTTTTATCTATTGTAATCTGCTATGTTCAAGTTCCGACTATGATTTTAATTGCAATTTGTACTGCAATCATCATGTCGCCTGAATTTTAGATGAGAAAGATCCTTTATGTTGCAGAAGTGTCGGATCAGAACTTTGTTGATGGAGAAAGGGAAGTTGAAAAAGTTGCAAAGGGTAGCAATGATTGCAATACCAAAAGGGTAAAAACTTCAGAAACCAATGATTTGATCAACGACCAGGTAATTCAAATGATATCAACTTAGCTATCATGTCCTTGCTCCAAAATGACAACCACTGTTTTCTTTTAGGTTGTTCCTTAA

mRNA sequence

ATGACAACTGTGAAGGTTCTTTTGTCCCCAAACACGGCCTTTCGCTCTCTTCCTAAAGATCTCTCTCCACTGGCCCGCTTCCATTTTTCTTCTCCATCTCTCTCTCTCTCTCGCCATTGCAAATCTGGAGATAATCCTTCATCTTCTTCTGTTTTCATGGAGCTTTTGCCTCCAAATTTAGAGAATCTCAAGCCTAAAGAAACTCGGACCGACTCACTGATCTCTGAATTGGCCATGGACGAGTCAACCGAGGCTCCCCTACAGGACGAATCTTCTAGCCAGGGCACTCGTATGACGGAGTCTGTACCTTCTGATTGGACCGATGAGAAGCACCATTTGTTCCTTGAATCCATGGAGGCATCTTTTGTCAGCCAGATGTTTGATTCTGCGCATTCAGGCGGTACCGGCTCCGGCAAGGAAAATTCATCATGGACAAAATTGCACGGCCAAAGCCAGGCCGCTTCTCATGTCCACTCACATGTTGGCCAGTTTAAGGTTCTTCGACAAGGCAGCTGGAAGAATGTTAATTTTGAACCAACTGAAGCTCGGCCGAATTTATTGAATGATTACCAGGCTCTATCGCGCAATCCATGGATACATCACTTCAGAGCTGCTCGCAGAAACAAAAATGTTGCATGTAAAAGTCCAGCCATCAGTTCAAGAAAAAGGAATTTGTTGCCATTTGGAGGAGATAATAATTCAGGACCTATGCGTGCTTGTAACTCTGATTTGTCTCAGCATTATATATACAACAATAAAGAAGTGTCGGATCAGAACTTTGTTGATGGAGAAAGGGAAGTTGAAAAAGTTGCAAAGGGTAGCAATGATTGCAATACCAAAAGGGTAAAAACTTCAGAAACCAATGATTTGATCAACGACCAGGTTGTTCCTTAA

Coding sequence (CDS)

ATGACAACTGTGAAGGTTCTTTTGTCCCCAAACACGGCCTTTCGCTCTCTTCCTAAAGATCTCTCTCCACTGGCCCGCTTCCATTTTTCTTCTCCATCTCTCTCTCTCTCTCGCCATTGCAAATCTGGAGATAATCCTTCATCTTCTTCTGTTTTCATGGAGCTTTTGCCTCCAAATTTAGAGAATCTCAAGCCTAAAGAAACTCGGACCGACTCACTGATCTCTGAATTGGCCATGGACGAGTCAACCGAGGCTCCCCTACAGGACGAATCTTCTAGCCAGGGCACTCGTATGACGGAGTCTGTACCTTCTGATTGGACCGATGAGAAGCACCATTTGTTCCTTGAATCCATGGAGGCATCTTTTGTCAGCCAGATGTTTGATTCTGCGCATTCAGGCGGTACCGGCTCCGGCAAGGAAAATTCATCATGGACAAAATTGCACGGCCAAAGCCAGGCCGCTTCTCATGTCCACTCACATGTTGGCCAGTTTAAGGTTCTTCGACAAGGCAGCTGGAAGAATGTTAATTTTGAACCAACTGAAGCTCGGCCGAATTTATTGAATGATTACCAGGCTCTATCGCGCAATCCATGGATACATCACTTCAGAGCTGCTCGCAGAAACAAAAATGTTGCATGTAAAAGTCCAGCCATCAGTTCAAGAAAAAGGAATTTGTTGCCATTTGGAGGAGATAATAATTCAGGACCTATGCGTGCTTGTAACTCTGATTTGTCTCAGCATTATATATACAACAATAAAGAAGTGTCGGATCAGAACTTTGTTGATGGAGAAAGGGAAGTTGAAAAAGTTGCAAAGGGTAGCAATGATTGCAATACCAAAAGGGTAAAAACTTCAGAAACCAATGATTTGATCAACGACCAGGTTGTTCCTTAA

Protein sequence

MTTVKVLLSPNTAFRSLPKDLSPLARFHFSSPSLSLSRHCKSGDNPSSSSVFMELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHHLFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSWKNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARRNKNVACKSPAISSRKRNLLPFGGDNNSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLINDQVVP
Homology
BLAST of Csor.00g141020 vs. ExPASy Swiss-Prot
Match: Q8L8T7 (Cold-regulated protein 27 OS=Arabidopsis thaliana OX=3702 GN=COR27 PE=1 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 9.7e-12
Identity = 66/217 (30.41%), Postives = 104/217 (47.93%), Query Frame = 0

Query: 89  DESSSQGTRMTESVPSDWTDEKHHLFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLH 148
           + +SS  +   E + ++WT+EKH L+L+SMEASFV Q+++S    G     EN S +   
Sbjct: 31  ETTSSMYSAGKEYMETEWTNEKHSLYLKSMEASFVDQLYNSL---GALGKNENVSESTRF 90

Query: 149 GQSQAASHVHSHVGQFKVLRQGSWKNVNFEPTEARPNLL---NDYQALSRNPWIHHFRAA 208
           G  +  S       QFKVL  G W+ +N +  E R N     N ++ L R+PWI H++  
Sbjct: 91  GSGRKPSQ-----EQFKVLHDGFWQKINVKQPEHRINGRHGGNSHEFL-RSPWIKHYKPL 150

Query: 209 RRNKNVACKSP-----AISSRKRNLLPFGGDNNSGPMRACNSDLSQHYIYNNKEVSDQNF 268
            + +      P     + S+ K+ +   G  ++   + + + D  Q  +    EVSDQNF
Sbjct: 151 VKTQIPVTDEPENQVVSSSNGKKGICSSGSASSLKQLSSHSRDHDQISV-GEAEVSDQNF 210

Query: 269 VDGEREVEKVAKGSNDCNTKRVKTSETNDLINDQVVP 298
           V+      +  KG N  + K      +     DQVVP
Sbjct: 211 VN------EGIKGENGSSKKMKTVMMSESSSTDQVVP 231

BLAST of Csor.00g141020 vs. ExPASy Swiss-Prot
Match: Q8L983 (Cold-regulated protein 28 OS=Arabidopsis thaliana OX=3702 GN=COR28 PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 2.7e-06
Identity = 62/235 (26.38%), Postives = 93/235 (39.57%), Query Frame = 0

Query: 71  DSLISELAMDESTEAPLQDESSSQGTRMTESVPSD--------WTDEKHHLFLESMEASF 130
           D  +SE + +  +E+ L +   S  T  T    +D        WT+EKH+ +L+ +E+SF
Sbjct: 15  DRDVSEASAESQSESTLSNSLDSGVTAETSRSDADSKLDECTAWTNEKHNSYLDYLESSF 74

Query: 131 VSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSWKNVNFEPTEA 190
           V Q++ S   GGT          +L       S+ H    QF VL+ G W+ VNF   ++
Sbjct: 75  VRQLY-SLLGGGT---------QRLSRTRDVQSNSHKSADQFTVLQNGCWQKVNFGKKQS 134

Query: 191 RPNLLNDYQALSRNPWIHHFRAARRNKNVACKSPAISSRKRNLLPFGGDNNSGPMRACNS 250
                     L  +      R + RNK                 P   + N         
Sbjct: 135 ---------CLETSSEFRFHRNSLRNK-----------------PENSNGNYTMGTTVQG 194

Query: 251 DLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLINDQVVP 298
           D+  H    + E S QNF + E E E   KG      +R   ++ + L  DQVVP
Sbjct: 195 DVLCHDETKHSEASGQNFREEEEEEE---KGEVSKKREREANNDDSSLKEDQVVP 210

BLAST of Csor.00g141020 vs. NCBI nr
Match: KAG6608473.1 (Cold-regulated protein 27, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 587 bits (1514), Expect = 1.84e-211
Identity = 297/297 (100.00%), Postives = 297/297 (100.00%), Query Frame = 0

Query: 1   MTTVKVLLSPNTAFRSLPKDLSPLARFHFSSPSLSLSRHCKSGDNPSSSSVFMELLPPNL 60
           MTTVKVLLSPNTAFRSLPKDLSPLARFHFSSPSLSLSRHCKSGDNPSSSSVFMELLPPNL
Sbjct: 1   MTTVKVLLSPNTAFRSLPKDLSPLARFHFSSPSLSLSRHCKSGDNPSSSSVFMELLPPNL 60

Query: 61  ENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHHLFLESMEA 120
           ENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHHLFLESMEA
Sbjct: 61  ENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHHLFLESMEA 120

Query: 121 SFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSWKNVNFEPT 180
           SFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSWKNVNFEPT
Sbjct: 121 SFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSWKNVNFEPT 180

Query: 181 EARPNLLNDYQALSRNPWIHHFRAARRNKNVACKSPAISSRKRNLLPFGGDNNSGPMRAC 240
           EARPNLLNDYQALSRNPWIHHFRAARRNKNVACKSPAISSRKRNLLPFGGDNNSGPMRAC
Sbjct: 181 EARPNLLNDYQALSRNPWIHHFRAARRNKNVACKSPAISSRKRNLLPFGGDNNSGPMRAC 240

Query: 241 NSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLINDQVVP 297
           NSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLINDQVVP
Sbjct: 241 NSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLINDQVVP 297

BLAST of Csor.00g141020 vs. NCBI nr
Match: XP_022982275.1 (uncharacterized protein LOC111481154 [Cucurbita maxima])

HSP 1 Score: 573 bits (1476), Expect = 1.14e-205
Identity = 290/297 (97.64%), Postives = 293/297 (98.65%), Query Frame = 0

Query: 1   MTTVKVLLSPNTAFRSLPKDLSPLARFHFSSPSLSLSRHCKSGDNPSSSSVFMELLPPNL 60
           MTTVKVLLSPNTAFRSLPKDLSPLARFHFS   LS SRHCKSGDNPSSSSVFMELLPPNL
Sbjct: 1   MTTVKVLLSPNTAFRSLPKDLSPLARFHFSFLHLSPSRHCKSGDNPSSSSVFMELLPPNL 60

Query: 61  ENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHHLFLESMEA 120
           ENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHHLFLESMEA
Sbjct: 61  ENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHHLFLESMEA 120

Query: 121 SFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSWKNVNFEPT 180
           SFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSWKNVNFEPT
Sbjct: 121 SFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSWKNVNFEPT 180

Query: 181 EARPNLLNDYQALSRNPWIHHFRAARRNKNVACKSPAISSRKRNLLPFGGDNNSGPMRAC 240
           EARPNLLNDYQALSRNPWIHHFRAAR+NKNVACKSPAISSRKRNLLPFGGDNNSGPMRAC
Sbjct: 181 EARPNLLNDYQALSRNPWIHHFRAARKNKNVACKSPAISSRKRNLLPFGGDNNSGPMRAC 240

Query: 241 NSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLINDQVVP 297
           NSDLS+HYIYNNKE+SDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLINDQVVP
Sbjct: 241 NSDLSRHYIYNNKEMSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLINDQVVP 297

BLAST of Csor.00g141020 vs. NCBI nr
Match: XP_023525276.1 (uncharacterized protein LOC111788927 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 482 bits (1241), Expect = 1.10e-170
Identity = 241/245 (98.37%), Postives = 244/245 (99.59%), Query Frame = 0

Query: 53  MELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 112
           MELLPPNLENLKPKETRTDSLIS LAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH
Sbjct: 1   MELLPPNLENLKPKETRTDSLISVLAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 60

Query: 113 LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 172
           LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW
Sbjct: 61  LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 120

Query: 173 KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARRNKNVACKSPAISSRKRNLLPFGGDN 232
           KNVNFEPTE+RPNLLNDY+ALSRNPWIHHFRAAR+NKNVACKSPAISSRKRNLLPFGGDN
Sbjct: 121 KNVNFEPTESRPNLLNDYRALSRNPWIHHFRAARKNKNVACKSPAISSRKRNLLPFGGDN 180

Query: 233 NSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN 292
           NSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN
Sbjct: 181 NSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN 240

Query: 293 DQVVP 297
           DQVVP
Sbjct: 241 DQVVP 245

BLAST of Csor.00g141020 vs. NCBI nr
Match: KAG7037801.1 (hypothetical protein SDJN02_01432 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 482 bits (1241), Expect = 3.82e-170
Identity = 241/243 (99.18%), Postives = 243/243 (100.00%), Query Frame = 0

Query: 53  MELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 112
           MELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH
Sbjct: 1   MELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 60

Query: 113 LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 172
           LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW
Sbjct: 61  LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 120

Query: 173 KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARRNKNVACKSPAISSRKRNLLPFGGDN 232
           KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARRNK+VACKSPAISSRKRNLLPFGGDN
Sbjct: 121 KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARRNKSVACKSPAISSRKRNLLPFGGDN 180

Query: 233 NSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN 292
           NSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN
Sbjct: 181 NSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN 240

Query: 293 DQV 295
           DQ+
Sbjct: 241 DQI 243

BLAST of Csor.00g141020 vs. NCBI nr
Match: XP_022940327.1 (uncharacterized protein LOC111445977 isoform X1 [Cucurbita moschata])

HSP 1 Score: 468 bits (1203), Expect = 5.89e-165
Identity = 237/245 (96.73%), Postives = 240/245 (97.96%), Query Frame = 0

Query: 53  MELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 112
           ME+LPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH
Sbjct: 1   MEVLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 60

Query: 113 LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 172
           LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW
Sbjct: 61  LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 120

Query: 173 KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARRNKNVACKSPAISSRKRNLLPFGGDN 232
           KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAAR+NKNVACKSPAISSRKRNLLPFG   
Sbjct: 121 KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARKNKNVACKSPAISSRKRNLLPFG--- 180

Query: 233 NSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN 292
            +GPMRACNSDLSQH IYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN
Sbjct: 181 -AGPMRACNSDLSQHCIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN 240

Query: 293 DQVVP 297
           DQVVP
Sbjct: 241 DQVVP 241

BLAST of Csor.00g141020 vs. ExPASy TrEMBL
Match: A0A6J1J278 (uncharacterized protein LOC111481154 OS=Cucurbita maxima OX=3661 GN=LOC111481154 PE=4 SV=1)

HSP 1 Score: 573 bits (1476), Expect = 5.53e-206
Identity = 290/297 (97.64%), Postives = 293/297 (98.65%), Query Frame = 0

Query: 1   MTTVKVLLSPNTAFRSLPKDLSPLARFHFSSPSLSLSRHCKSGDNPSSSSVFMELLPPNL 60
           MTTVKVLLSPNTAFRSLPKDLSPLARFHFS   LS SRHCKSGDNPSSSSVFMELLPPNL
Sbjct: 1   MTTVKVLLSPNTAFRSLPKDLSPLARFHFSFLHLSPSRHCKSGDNPSSSSVFMELLPPNL 60

Query: 61  ENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHHLFLESMEA 120
           ENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHHLFLESMEA
Sbjct: 61  ENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHHLFLESMEA 120

Query: 121 SFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSWKNVNFEPT 180
           SFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSWKNVNFEPT
Sbjct: 121 SFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSWKNVNFEPT 180

Query: 181 EARPNLLNDYQALSRNPWIHHFRAARRNKNVACKSPAISSRKRNLLPFGGDNNSGPMRAC 240
           EARPNLLNDYQALSRNPWIHHFRAAR+NKNVACKSPAISSRKRNLLPFGGDNNSGPMRAC
Sbjct: 181 EARPNLLNDYQALSRNPWIHHFRAARKNKNVACKSPAISSRKRNLLPFGGDNNSGPMRAC 240

Query: 241 NSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLINDQVVP 297
           NSDLS+HYIYNNKE+SDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLINDQVVP
Sbjct: 241 NSDLSRHYIYNNKEMSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLINDQVVP 297

BLAST of Csor.00g141020 vs. ExPASy TrEMBL
Match: A0A6J1FJA4 (uncharacterized protein LOC111445977 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445977 PE=4 SV=1)

HSP 1 Score: 468 bits (1203), Expect = 2.85e-165
Identity = 237/245 (96.73%), Postives = 240/245 (97.96%), Query Frame = 0

Query: 53  MELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 112
           ME+LPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH
Sbjct: 1   MEVLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 60

Query: 113 LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 172
           LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW
Sbjct: 61  LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 120

Query: 173 KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARRNKNVACKSPAISSRKRNLLPFGGDN 232
           KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAAR+NKNVACKSPAISSRKRNLLPFG   
Sbjct: 121 KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARKNKNVACKSPAISSRKRNLLPFG--- 180

Query: 233 NSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN 292
            +GPMRACNSDLSQH IYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN
Sbjct: 181 -AGPMRACNSDLSQHCIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN 240

Query: 293 DQVVP 297
           DQVVP
Sbjct: 241 DQVVP 241

BLAST of Csor.00g141020 vs. ExPASy TrEMBL
Match: A0A6J1FNZ0 (uncharacterized protein LOC111445977 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445977 PE=4 SV=1)

HSP 1 Score: 386 bits (992), Expect = 7.75e-134
Identity = 193/200 (96.50%), Postives = 195/200 (97.50%), Query Frame = 0

Query: 98  MTESVPSDWTDEKHHLFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHV 157
           MTESVPSDWTDEKHHLFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHV
Sbjct: 1   MTESVPSDWTDEKHHLFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHV 60

Query: 158 HSHVGQFKVLRQGSWKNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARRNKNVACKSPA 217
           HSHVGQFKVLRQGSWKNVNFEPTEARPNLLNDYQALSRNPWIHHFRAAR+NKNVACKSPA
Sbjct: 61  HSHVGQFKVLRQGSWKNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARKNKNVACKSPA 120

Query: 218 ISSRKRNLLPFGGDNNSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDC 277
           ISSRKRNLLPFG    +GPMRACNSDLSQH IYNNKEVSDQNFVDGEREVEKVAKGSNDC
Sbjct: 121 ISSRKRNLLPFG----AGPMRACNSDLSQHCIYNNKEVSDQNFVDGEREVEKVAKGSNDC 180

Query: 278 NTKRVKTSETNDLINDQVVP 297
           NTKRVKTSETNDLINDQVVP
Sbjct: 181 NTKRVKTSETNDLINDQVVP 196

BLAST of Csor.00g141020 vs. ExPASy TrEMBL
Match: A0A6J1CJ78 (uncharacterized protein LOC111011825 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111011825 PE=4 SV=1)

HSP 1 Score: 343 bits (880), Expect = 4.33e-116
Identity = 182/246 (73.98%), Postives = 205/246 (83.33%), Query Frame = 0

Query: 53  MELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 112
           ME  PPN+EN+K  E +TDSL  E A D++TE PL  ESSSQGT+MTESVPSDWTDEKHH
Sbjct: 1   MEFFPPNMENIKAAEIQTDSLPLESAEDDATEFPLPMESSSQGTQMTESVPSDWTDEKHH 60

Query: 113 LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 172
            FLESMEASFVSQMFDSAHS G+  GKENSS TKLHGQ+Q+AS++HSH GQFKVLR+GSW
Sbjct: 61  RFLESMEASFVSQMFDSAHSVGS-VGKENSSRTKLHGQTQSASNIHSHFGQFKVLRRGSW 120

Query: 173 KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARRNKNVACKSPAISSRKRNL-LPFGGD 232
           KN+NFEPTE+RP++LND QALSRNPWIHHFRAAR+ KNVACKS AISS +RN   PFG  
Sbjct: 121 KNINFEPTESRPSVLNDLQALSRNPWIHHFRAARKQKNVACKSQAISSGERNSSFPFGST 180

Query: 233 NNSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLI 292
           NNSGPMRAC+S LSQ YI +N+EVSDQNFVD +REVEK   G+N C  KRV T ETN L 
Sbjct: 181 NNSGPMRACDSHLSQQYISSNREVSDQNFVDEDREVEK---GNNGCIAKRVNTPETNALS 240

Query: 293 NDQVVP 297
           NDQVVP
Sbjct: 241 NDQVVP 242

BLAST of Csor.00g141020 vs. ExPASy TrEMBL
Match: A0A6J1FBV0 (uncharacterized protein LOC111442637 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111442637 PE=4 SV=1)

HSP 1 Score: 340 bits (871), Expect = 1.08e-114
Identity = 182/243 (74.90%), Postives = 198/243 (81.48%), Query Frame = 0

Query: 53  MELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 112
           MELLPPNLEN    ETRTDSLISELA DESTE  L  ESSS GT MT+SVPSDWTDEKHH
Sbjct: 1   MELLPPNLENHNAVETRTDSLISELAKDESTEFALPVESSSLGTHMTDSVPSDWTDEKHH 60

Query: 113 LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 172
           LFLESME SFVSQMFDS HS G+   KENSS TKLHGQSQ+ASHVHSH GQFKVLR+GSW
Sbjct: 61  LFLESMETSFVSQMFDSVHSVGSCPRKENSSHTKLHGQSQSASHVHSHFGQFKVLRRGSW 120

Query: 173 KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARRNKNVACKSPAISSRKRNLLPFGGDN 232
           KN+NFE TE+R NLLND+QALSRNPWIHHFRA R+NKNVACKS AI S  RNL P G  N
Sbjct: 121 KNINFETTESRSNLLNDFQALSRNPWIHHFRADRKNKNVACKSQAIGSGGRNLFPLGAAN 180

Query: 233 NSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDC-NTKRVKTSETNDLI 292
           +S P+RAC+SDLS  YI +N+EVSDQNFVD +RE   V  GS+DC N KRV T ETN LI
Sbjct: 181 DSEPLRACDSDLSLQYISSNEEVSDQNFVDEDRE---VGNGSSDCSNAKRVNTPETNALI 240

Query: 293 NDQ 294
           N+Q
Sbjct: 241 NEQ 240

BLAST of Csor.00g141020 vs. TAIR 10
Match: AT5G42900.3 (cold regulated gene 27 )

HSP 1 Score: 73.6 bits (179), Expect = 3.1e-13
Identity = 67/217 (30.88%), Postives = 105/217 (48.39%), Query Frame = 0

Query: 89  DESSSQGTRMTESVPSDWTDEKHHLFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLH 148
           + +SS  +   E + ++WT+EKH L+L+SMEASFV Q+++S    G     EN S +   
Sbjct: 31  ETTSSMYSAGKEYMETEWTNEKHSLYLKSMEASFVDQLYNSL---GALGKNENVSESTRF 90

Query: 149 GQSQAASHVHSHVGQFKVLRQGSWKNVNFEPTEARPNLL---NDYQALSRNPWIHHFRAA 208
           G  +  S       QFKVL  G W+ +N +  E R N     N ++ L R+PWI H++  
Sbjct: 91  GSGRKPSQ-----EQFKVLHDGFWQKINVKQPEHRINGRHGGNSHEFL-RSPWIKHYKPL 150

Query: 209 RRNKNVACKSP-----AISSRKRNLLPFGGDNNSGPMRACNSDLSQHYIYNNKEVSDQNF 268
            + +      P     + S+ K+ +   G  ++   + + + D  Q  I   +EVSDQNF
Sbjct: 151 VKTQIPVTDEPENQVVSSSNGKKGICSSGSASSLKQLSSHSRDHDQ--ISVGEEVSDQNF 210

Query: 269 VDGEREVEKVAKGSNDCNTKRVKTSETNDLINDQVVP 298
           V+      +  KG N  + K      +     DQVVP
Sbjct: 211 VN------EGIKGENGSSKKMKTVMMSESSSTDQVVP 230

BLAST of Csor.00g141020 vs. TAIR 10
Match: AT5G42900.1 (cold regulated gene 27 )

HSP 1 Score: 72.4 bits (176), Expect = 6.9e-13
Identity = 66/217 (30.41%), Postives = 104/217 (47.93%), Query Frame = 0

Query: 89  DESSSQGTRMTESVPSDWTDEKHHLFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLH 148
           + +SS  +   E + ++WT+EKH L+L+SMEASFV Q+++S    G     EN S +   
Sbjct: 31  ETTSSMYSAGKEYMETEWTNEKHSLYLKSMEASFVDQLYNSL---GALGKNENVSESTRF 90

Query: 149 GQSQAASHVHSHVGQFKVLRQGSWKNVNFEPTEARPNLL---NDYQALSRNPWIHHFRAA 208
           G  +  S       QFKVL  G W+ +N +  E R N     N ++ L R+PWI H++  
Sbjct: 91  GSGRKPSQ-----EQFKVLHDGFWQKINVKQPEHRINGRHGGNSHEFL-RSPWIKHYKPL 150

Query: 209 RRNKNVACKSP-----AISSRKRNLLPFGGDNNSGPMRACNSDLSQHYIYNNKEVSDQNF 268
            + +      P     + S+ K+ +   G  ++   + + + D  Q  +    EVSDQNF
Sbjct: 151 VKTQIPVTDEPENQVVSSSNGKKGICSSGSASSLKQLSSHSRDHDQISV-GEAEVSDQNF 210

Query: 269 VDGEREVEKVAKGSNDCNTKRVKTSETNDLINDQVVP 298
           V+      +  KG N  + K      +     DQVVP
Sbjct: 211 VN------EGIKGENGSSKKMKTVMMSESSSTDQVVP 231

BLAST of Csor.00g141020 vs. TAIR 10
Match: AT5G42900.2 (cold regulated gene 27 )

HSP 1 Score: 72.4 bits (176), Expect = 6.9e-13
Identity = 66/217 (30.41%), Postives = 104/217 (47.93%), Query Frame = 0

Query: 89  DESSSQGTRMTESVPSDWTDEKHHLFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLH 148
           + +SS  +   E + ++WT+EKH L+L+SMEASFV Q+++S    G     EN S +   
Sbjct: 31  ETTSSMYSAGKEYMETEWTNEKHSLYLKSMEASFVDQLYNSL---GALGKNENVSESTRF 90

Query: 149 GQSQAASHVHSHVGQFKVLRQGSWKNVNFEPTEARPNLL---NDYQALSRNPWIHHFRAA 208
           G  +  S       QFKVL  G W+ +N +  E R N     N ++ L R+PWI H++  
Sbjct: 91  GSGRKPSQ-----EQFKVLHDGFWQKINVKQPEHRINGRHGGNSHEFL-RSPWIKHYKPL 150

Query: 209 RRNKNVACKSP-----AISSRKRNLLPFGGDNNSGPMRACNSDLSQHYIYNNKEVSDQNF 268
            + +      P     + S+ K+ +   G  ++   + + + D  Q  +    EVSDQNF
Sbjct: 151 VKTQIPVTDEPENQVVSSSNGKKGICSSGSASSLKQLSSHSRDHDQISV-GEAEVSDQNF 210

Query: 269 VDGEREVEKVAKGSNDCNTKRVKTSETNDLINDQVVP 298
           V+      +  KG N  + K      +     DQVVP
Sbjct: 211 VN------EGIKGENGSSKKMKTVMMSESSSTDQVVP 231

BLAST of Csor.00g141020 vs. TAIR 10
Match: AT4G33980.1 (BEST Arabidopsis thaliana protein match is: cold regulated gene 27 (TAIR:AT5G42900.2); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 54.3 bits (129), Expect = 1.9e-07
Identity = 62/235 (26.38%), Postives = 93/235 (39.57%), Query Frame = 0

Query: 71  DSLISELAMDESTEAPLQDESSSQGTRMTESVPSD--------WTDEKHHLFLESMEASF 130
           D  +SE + +  +E+ L +   S  T  T    +D        WT+EKH+ +L+ +E+SF
Sbjct: 15  DRDVSEASAESQSESTLSNSLDSGVTAETSRSDADSKLDECTAWTNEKHNSYLDYLESSF 74

Query: 131 VSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSWKNVNFEPTEA 190
           V Q++ S   GGT          +L       S+ H    QF VL+ G W+ VNF   ++
Sbjct: 75  VRQLY-SLLGGGT---------QRLSRTRDVQSNSHKSADQFTVLQNGCWQKVNFGKKQS 134

Query: 191 RPNLLNDYQALSRNPWIHHFRAARRNKNVACKSPAISSRKRNLLPFGGDNNSGPMRACNS 250
                     L  +      R + RNK                 P   + N         
Sbjct: 135 ---------CLETSSEFRFHRNSLRNK-----------------PENSNGNYTMGTTVQG 194

Query: 251 DLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLINDQVVP 298
           D+  H    + E S QNF + E E E   KG      +R   ++ + L  DQVVP
Sbjct: 195 DVLCHDETKHSEASGQNFREEEEEEE---KGEVSKKREREANNDDSSLKEDQVVP 210

BLAST of Csor.00g141020 vs. TAIR 10
Match: AT4G33980.2 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: response to karrikin; LOCATED IN: cellular_component unknown; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: cold regulated gene 27 (TAIR:AT5G42900.3). )

HSP 1 Score: 54.3 bits (129), Expect = 1.9e-07
Identity = 62/235 (26.38%), Postives = 93/235 (39.57%), Query Frame = 0

Query: 71  DSLISELAMDESTEAPLQDESSSQGTRMTESVPSD--------WTDEKHHLFLESMEASF 130
           D  +SE + +  +E+ L +   S  T  T    +D        WT+EKH+ +L+ +E+SF
Sbjct: 15  DRDVSEASAESQSESTLSNSLDSGVTAETSRSDADSKLDECTAWTNEKHNSYLDYLESSF 74

Query: 131 VSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSWKNVNFEPTEA 190
           V Q++ S   GGT          +L       S+ H    QF VL+ G W+ VNF   ++
Sbjct: 75  VRQLY-SLLGGGT---------QRLSRTRDVQSNSHKSADQFTVLQNGCWQKVNFGKKQS 134

Query: 191 RPNLLNDYQALSRNPWIHHFRAARRNKNVACKSPAISSRKRNLLPFGGDNNSGPMRACNS 250
                     L  +      R + RNK                 P   + N         
Sbjct: 135 ---------CLETSSEFRFHRNSLRNK-----------------PENSNGNYTMGTTVQG 194

Query: 251 DLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLINDQVVP 298
           D+  H    + E S QNF + E E E   KG      +R   ++ + L  DQVVP
Sbjct: 195 DVLCHDETKHSEASGQNFREEEEEEE---KGEVSKKREREANNDDSSLKEDQVVP 210

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L8T79.7e-1230.41Cold-regulated protein 27 OS=Arabidopsis thaliana OX=3702 GN=COR27 PE=1 SV=1[more]
Q8L9832.7e-0626.38Cold-regulated protein 28 OS=Arabidopsis thaliana OX=3702 GN=COR28 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAG6608473.11.84e-211100.00Cold-regulated protein 27, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022982275.11.14e-20597.64uncharacterized protein LOC111481154 [Cucurbita maxima][more]
XP_023525276.11.10e-17098.37uncharacterized protein LOC111788927 isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG7037801.13.82e-17099.18hypothetical protein SDJN02_01432 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022940327.15.89e-16596.73uncharacterized protein LOC111445977 isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1J2785.53e-20697.64uncharacterized protein LOC111481154 OS=Cucurbita maxima OX=3661 GN=LOC111481154... [more]
A0A6J1FJA42.85e-16596.73uncharacterized protein LOC111445977 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1FNZ07.75e-13496.50uncharacterized protein LOC111445977 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1CJ784.33e-11673.98uncharacterized protein LOC111011825 isoform X3 OS=Momordica charantia OX=3673 G... [more]
A0A6J1FBV01.08e-11474.90uncharacterized protein LOC111442637 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT5G42900.33.1e-1330.88cold regulated gene 27 [more]
AT5G42900.16.9e-1330.41cold regulated gene 27 [more]
AT5G42900.26.9e-1330.41cold regulated gene 27 [more]
AT4G33980.11.9e-0726.38BEST Arabidopsis thaliana protein match is: cold regulated gene 27 (TAIR:AT5G429... [more]
AT4G33980.21.9e-0726.38FUNCTIONS IN: molecular_function unknown; INVOLVED IN: response to karrikin; LOC... [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 77..105
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 85..102
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 129..153
NoneNo IPR availablePANTHERPTHR33676:SF3COLD REGULATED PROTEIN 27coord: 69..297
IPR044678Cold-regulated protein 27/28PANTHERPTHR33676COLD REGULATED PROTEIN 27coord: 69..297

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g141020.m01Csor.00g141020.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042752 regulation of circadian rhythm
biological_process GO:0009409 response to cold