Csor.00g136000 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g136000
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionPectin lyase-like superfamily protein
LocationCsor_Chr12: 91610 .. 95212 (-)
RNA-Seq ExpressionCsor.00g136000
SyntenyCsor.00g136000
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATAGAAATGAAACGATTGGAAGCAAAATTGCGAAGTCGTTGGTGGATCTCGAAGCTGAAAGATGTTGCCATTATTTTGCAGGCTTCGAGCTTCTTCTCTCAGCTGGATTTGCATGTTGTGACTTGGTGCCATTGGTGGGGTTCAGGAAGCAAATGGTGAGCAGGTCCCTGTACTTGTTCAATTTTTATACTGGGAAATTTGGTGTCTGAAATTTGGTGTCTGTGACAGTATGCCTGGCTGTGTGGCGTGGTGGAAAATGGATTTAAATTGAAGCACAGCCTTCAACTAGAATGGATGTACTCATTCTACAAAGGTGGCCTTTCAACTAAATGAATATATGATTAGGTACAAACAATAACAGTCACAGTCACACACATATTGTACACTCTCTCGCTTTATTTTTATATATTGTAGATTAAAAGCCTGCCATTAACAAATTGGTATGTTGGCTAGCCTTTGTCTGCACAGTCTCCTGAAGATTACGGAGTCGCTACCTATTTAACCCCACTTCACCTCCCACTTGCTTTGCCGATCTCAAAAGAACACTACCTCTCTCTCTCTCCCTGCCTTCCCACATTTACACTAAAAGTGAGGAGTGGGATGAGTTCCAAAACCTTAACACTTTCGGTTTTTATAGCTTTTCTTGTTTGGTCTTCAAACTTTGAAGGCTGCACTGCAAGGAGAGGAAAACACTGGAGACAGAGCAGGCAGACCTCGGCTTCTCTATCAAAGAAGAAAGGCAGTCATGGCGGTAGCAAGAGCCACCATGTCAGTGGCTCCAACCCAAAAGCTCCTCCGTCACACAATGCCTCCCCTTCGTCTCCAATTCCTCCTAAACCAAAAGAAGAGATCATTCCAACTCCACCCAAGAAAGGCTACAAGGGTAGCGATTCTGCCATATTCAACGTTCTACATTTTGGTGCTAAAGGCAATGGAGAGTCTGATGACACTAAGGTACGTTCAATCACTCTTCTTCTGTAGAAATACTTGGAGTTTGATATCTAACAGAATTTTGTCATTGCAAAAGGATTGTATTCCAACAACTACAAACAAATTGATTTTATGTTACTTGTTGTAACTTTCAGAAACTTCAAGAACATATCTTAATTATCCTAAGTGGATAATTTTGGTGCTACAAATAATAATAGTAATAGTGCATATCATCATTTGTATTTGAAAGACCAGCTTAAAACCCTCGCTCCCAAGGGCGAGCCGGTCTTCAGACCGACAACCAGAGCCGGCGGGACCCACAGCCAATAATAGAAAATAATTTAGTTATGCCAGTTTTCTTGAATAAATGGAATACCATCAAGGAATGATTTTGATTGGAAAATATTTGCACGATCCGTTCCAGGCATTTGAAGACGCTTGGGTAGCCGCTTGTAAGGTGGAGGGATCGACGGTCATGGTTCCGGCAGACTATGTGTTCTTCGTAGGACCCATTTCCTTCTCTGGCCCATATTGCCAACCAGATATTGTATTTCAGGTAAATATTATATTAGCTCATTAGACTTCATTTGCAGCTGGATGGAACCATTATGGCTCCCACTGATCACCGAACTTGGGGGAAAGGTCTCTTGGAATGGATCCAATTTACAAAGCTAATAGGGGTTACCATACGGGGAAGTGGCATAATCGACGGAAGAGGATCCGTCTGGTGGCAAGATTCCTCCTTTGATGAGCCTATAGACGACGAGATGAAACTCATTATCCCCATGAACAAGACCAGAGATGAAGCAGAACCAACGCCGGTAATGAACACTATCTATACTGATCTTTTCCTCCACAATATTCAGTTTTCTAGCTGCAAATAGAGAAGGAAAATGAACGTGATTCACTAAAAAATATGTGGCAGGTAGGAAGCAATCTAGAAGGCAAAATGCCCAGCATCAAGCCAACTGTAAGGGATCACTTACTGATAAACTTCTAAGATGATTTCATTATGGCCCCATCATCCTCACCCTGTGGAGTTGTATGAAATGTTATGTTACGCAGGCACTAAGGTTTTATGGAAGCTTCAATGTTACAGTCACGGGCATAACAATCCAAAACAGCCCCCAATGCCACCTCAAGTTTGACAACTGCGTGGGAGTCCTGGTGCATGATATCAGCGTCTCATCTCCTGGAAACAGTCCTAATACTGATGGAATTCAGTTGCAAAACTCCAAAGACGTTCTAATCCACAGCACCAGTCTATCTTGTGGTAATTACGTCACTTGCAACCACTTGATCCTGTTGTTAAATATTATCTACTTGAATGGGCGGTGCTCAGTAAGGGCTAAGGGTGTGCATGTCTTGAGATAAACTGAGCACTCAACATTCAATCGTTACGACTATTTAAAAGGAGCATTTGATGGACAAAATACTAAATGTTTACTTAAAATATGATAACCTTTCTTGTCACTAGACATAAGAGTCTTTCACTGCAGCAATTTGCAACCATTAGTTATAAAGGCCAAGTTGAAATACTCAACAATTTTGAACATTGGTTTTAATAGAAGAAGAAGAAAAAAAAAAACAATAATGTAGATTACGAGTAGGTAGAAGTTTGAAGTTGAAAATTGAAATAAACAGGTGAGTGATGATCTTTATTCTGATCCCTTCAGGAGATGATTGTGTTTCCATTCAAACTGGATGCTCGAACATATACATACACAACGTGAACTGCGGGCCAGGACATGGAATCAGCATTGGGAGTCTAGGAAAGGACAATACAAAAGCTTGTGTTTCAAACATAACTGTCCGTGATGTCACAATGCATAACACGATGAACGGTGTTAGAATCAAAACATGGCAGGTACTTGTGTTTAAAATTGGAAAGCATGTTGTTCATACCAAATGAACAAGCTCATTATTTTTCGGACAACCTTGCATTTTTCTAAAATTGGGAAGCCCCATAAGTCATCTATAATGTTCAGAATTGAATATCCACGAGTATTGATAGAATGGCACATGATACCTTTACAGGGTGGGTCAGGCTCTGTTCAGAACGTTCTCTTCTCTCAAACATTCAAATGTCCGAAGTTCAATTACCAATTGTGATTGACCAATTTTATTGCGACAAAGCGAAATGCAGTAACCAGACTTCAGGCGTGGCTCTATCAGGAATCGATTATGAGAAAATCCGAGGGACTTACACAGTTAAGCCTGTACACTTTGCCTGTAGCGACAACCAACCTTGTAGGGATGTAACGCCGACAACCATAGAGCTAAAACCACTTCAAGAGCGGTATCATTTATATGATCCTTTCTGCTGGCAGACATTTGGAGAACTGAAAACTCCTACTTCACCTCCAATTGGATGCTTACAGATTGGGAAGCCGTCTAGCAACAGAGTTCAGTACGAAGAAAATGATTCCTGTTAAGCTTAGAGAGGAACATTAAAAAGTTTCAGAATCAAGGCATTACTGAACAGGGTTTCAGGAGGCCATGGATGTCTCAAAAGTATGGCTCTTATCGTCATGGCTCTAGTTGTTGGTGCTGCAGTCATGTCCCAAAACATAGAGTCTTCGGATTGGGAGGAACTCACTGCTTTCTGTGCTGCCCTTCCTGCATTGTCAGCTGACGTTTCGTATTTCGATACTTTCCCCCTCCGATGA

mRNA sequence

ATGCATAGAAATGAAACGATTGGAAGCAAAATTGCGAAGTCGTTGGTGGATCTCGAAGCTGAAAGATGTTGCCATTATTTTGCAGGCTTCGAGCTTCTTCTCTCAGCTGGATTTGCATGTTGTGACTTGGTGCCATTGGTGGGGTTCAGGAAGCAAATGTATGCCTGGCTGTGTGGCGTGGTGGAAAATGGATTTAAATTGAAGCACAGCCTTCAACTAGAATGGATGTACTCATTCTACAAAGGCTGCACTGCAAGGAGAGGAAAACACTGGAGACAGAGCAGGCAGACCTCGGCTTCTCTATCAAAGAAGAAAGGCAGTCATGGCGGTAGCAAGAGCCACCATGTCAGTGGCTCCAACCCAAAAGCTCCTCCGTCACACAATGCCTCCCCTTCGTCTCCAATTCCTCCTAAACCAAAAGAAGAGATCATTCCAACTCCACCCAAGAAAGGCTACAAGGGTAGCGATTCTGCCATATTCAACGTTCTACATTTTGGTGCTAAAGGCAATGGAGAGTCTGATGACACTAAGGCATTTGAAGACGCTTGGGTAGCCGCTTGTAAGGTGGAGGGATCGACGGTCATGGTTCCGGCAGACTATGTGTTCTTCCTGGATGGAACCATTATGGCTCCCACTGATCACCGAACTTGGGGGAAAGGTCTCTTGGAATGGATCCAATTTACAAAGCTAATAGGGGTTACCATACGGGGAAGTGGCATAATCGACGGAAGAGGATCCGTCTGGTGGCAAGATTCCTCCTTTGATGAGCCTATAGACGACGAGATGAAACTCATTATCCCCATGAACAAGACCAGAGATGAAGCAGAACCAACGCCGGTAGGAAGCAATCTAGAAGGCAAAATGCCCAGCATCAAGCCAACTGCACTAAGGTTTTATGGAAGCTTCAATGTTACAGTCACGGGCATAACAATCCAAAACAGCCCCCAATGCCACCTCAAGTTTGACAACTGCGTGGGAGTCCTGGTGCATGATATCAGCGTCTCATCTCCTGGAAACAGTCCTAATACTGATGGAATTCAGTTGCAAAACTCCAAAGACGTTCTAATCCACAGCACCAGTCTATCTTGTGGGTGGGTCAGGCTCTGTTCAGAACGTTCTCTTCTCTCAAACATTCAAATGTCCGAAGTTCAATTACCAATTGTGATTGACCAATTTTATTGCGACAAAGCGAAATGCAGTAACCAGACTTCAGGCGTGGCTCTATCAGGAATCGATTATGAGAAAATCCGAGGGACTTACACAGTTAAGCCTGTACACTTTGCCTGTAGCGACAACCAACCTTGTAGGGATGTAACGCCGACAACCATAGAGCTAAAACCACTTCAAGAGCGGTATCATTTATATGATCCTTTCTGCTGGCAGACATTTGGAGAACTGAAAACTCCTACTTCACCTCCAATTGGATGCTTACAGATTGGGAAGCCGTCTAGCAACAGAGTTCAGGTTTCAGGAGGCCATGGATGTCTCAAAAGTATGGCTCTTATCGTCATGGCTCTAGTTGTTGGTGCTGCAGTCATGTCCCAAAACATAGAGTCTTCGGATTGGGAGGAACTCACTGCTTTCTGTGCTGCCCTTCCTGCATTGTCAGCTGACGTTTCGTATTTCGATACTTTCCCCCTCCGATGA

Coding sequence (CDS)

ATGCATAGAAATGAAACGATTGGAAGCAAAATTGCGAAGTCGTTGGTGGATCTCGAAGCTGAAAGATGTTGCCATTATTTTGCAGGCTTCGAGCTTCTTCTCTCAGCTGGATTTGCATGTTGTGACTTGGTGCCATTGGTGGGGTTCAGGAAGCAAATGTATGCCTGGCTGTGTGGCGTGGTGGAAAATGGATTTAAATTGAAGCACAGCCTTCAACTAGAATGGATGTACTCATTCTACAAAGGCTGCACTGCAAGGAGAGGAAAACACTGGAGACAGAGCAGGCAGACCTCGGCTTCTCTATCAAAGAAGAAAGGCAGTCATGGCGGTAGCAAGAGCCACCATGTCAGTGGCTCCAACCCAAAAGCTCCTCCGTCACACAATGCCTCCCCTTCGTCTCCAATTCCTCCTAAACCAAAAGAAGAGATCATTCCAACTCCACCCAAGAAAGGCTACAAGGGTAGCGATTCTGCCATATTCAACGTTCTACATTTTGGTGCTAAAGGCAATGGAGAGTCTGATGACACTAAGGCATTTGAAGACGCTTGGGTAGCCGCTTGTAAGGTGGAGGGATCGACGGTCATGGTTCCGGCAGACTATGTGTTCTTCCTGGATGGAACCATTATGGCTCCCACTGATCACCGAACTTGGGGGAAAGGTCTCTTGGAATGGATCCAATTTACAAAGCTAATAGGGGTTACCATACGGGGAAGTGGCATAATCGACGGAAGAGGATCCGTCTGGTGGCAAGATTCCTCCTTTGATGAGCCTATAGACGACGAGATGAAACTCATTATCCCCATGAACAAGACCAGAGATGAAGCAGAACCAACGCCGGTAGGAAGCAATCTAGAAGGCAAAATGCCCAGCATCAAGCCAACTGCACTAAGGTTTTATGGAAGCTTCAATGTTACAGTCACGGGCATAACAATCCAAAACAGCCCCCAATGCCACCTCAAGTTTGACAACTGCGTGGGAGTCCTGGTGCATGATATCAGCGTCTCATCTCCTGGAAACAGTCCTAATACTGATGGAATTCAGTTGCAAAACTCCAAAGACGTTCTAATCCACAGCACCAGTCTATCTTGTGGGTGGGTCAGGCTCTGTTCAGAACGTTCTCTTCTCTCAAACATTCAAATGTCCGAAGTTCAATTACCAATTGTGATTGACCAATTTTATTGCGACAAAGCGAAATGCAGTAACCAGACTTCAGGCGTGGCTCTATCAGGAATCGATTATGAGAAAATCCGAGGGACTTACACAGTTAAGCCTGTACACTTTGCCTGTAGCGACAACCAACCTTGTAGGGATGTAACGCCGACAACCATAGAGCTAAAACCACTTCAAGAGCGGTATCATTTATATGATCCTTTCTGCTGGCAGACATTTGGAGAACTGAAAACTCCTACTTCACCTCCAATTGGATGCTTACAGATTGGGAAGCCGTCTAGCAACAGAGTTCAGGTTTCAGGAGGCCATGGATGTCTCAAAAGTATGGCTCTTATCGTCATGGCTCTAGTTGTTGGTGCTGCAGTCATGTCCCAAAACATAGAGTCTTCGGATTGGGAGGAACTCACTGCTTTCTGTGCTGCCCTTCCTGCATTGTCAGCTGACGTTTCGTATTTCGATACTTTCCCCCTCCGATGA

Protein sequence

MHRNETIGSKIAKSLVDLEAERCCHYFAGFELLLSAGFACCDLVPLVGFRKQMYAWLCGVVENGFKLKHSLQLEWMYSFYKGCTARRGKHWRQSRQTSASLSKKKGSHGGSKSHHVSGSNPKAPPSHNASPSSPIPPKPKEEIIPTPPKKGYKGSDSAIFNVLHFGAKGNGESDDTKAFEDAWVAACKVEGSTVMVPADYVFFLDGTIMAPTDHRTWGKGLLEWIQFTKLIGVTIRGSGIIDGRGSVWWQDSSFDEPIDDEMKLIIPMNKTRDEAEPTPVGSNLEGKMPSIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPNTDGIQLQNSKDVLIHSTSLSCGWVRLCSERSLLSNIQMSEVQLPIVIDQFYCDKAKCSNQTSGVALSGIDYEKIRGTYTVKPVHFACSDNQPCRDVTPTTIELKPLQERYHLYDPFCWQTFGELKTPTSPPIGCLQIGKPSSNRVQVSGGHGCLKSMALIVMALVVGAAVMSQNIESSDWEELTAFCAALPALSADVSYFDTFPLR
Homology
BLAST of Csor.00g136000 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 5.1e-59
Identity = 156/475 (32.84%), Postives = 212/475 (44.63%), Query Frame = 0

Query: 105 KGSHGGSKSHHVSGSNPKAPPSHNASPSSPIPPKPKEEIIPTPPKKGYKGSDSAIFNVLH 164
           KG HG   +H +    P  PP   A+P   +P  P     P+P           +F+V  
Sbjct: 33  KGRHG--VTHPL--PPPPPPPLETANPPDQVPSDP----YPSPDPAPGDSDSGCVFDVTS 92

Query: 165 FGAKGNGESDDTKAFEDAWVAACKVEGSTVMVP-----------------ADYVFFLDGT 224
           FGA G+G  DDT AF+DAW AAC VE   V+ P                    VF LDG 
Sbjct: 93  FGAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGGVFKITSTIFSGPCKPGLVFQLDGV 152

Query: 225 IMAPTDHRTWGK--GLLEWIQFTKLIGVTIRGSGIIDGRGSVWWQDSSFDEPIDDEMKLI 284
           +M P     W +     +W+ F +L G T  G G ++G G  WW                
Sbjct: 153 LMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWD--------------- 212

Query: 285 IPMNKTRDEAEPTPVGSNLEGKMPSIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCV 344
           +P    R      P GS+  G  P   PT +RF+ S N+ V G+ IQNSPQ H+KFD C 
Sbjct: 213 LPCKPHRG-----PDGSSSSG--PCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQ 272

Query: 345 GVLVHDISVSSPGNSPNTDGIQLQNSKDVLIHSTSLSCGWVRL-----CSE--------- 404
           GVL+++I +SSP  SPNTDGI L N++ V I+++ +S G   +     CS+         
Sbjct: 273 GVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCG 332

Query: 405 -------------------------------------------------RSLLSNIQMSE 464
                                                              L  NIQM  
Sbjct: 333 PSHGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMEN 392

Query: 465 VQLPIVIDQFYCDKAKCSNQTSGVALSGIDYEKIRGTYTVK--PVHFACSDNQPCRDVTP 496
           V   I++DQ+YC    C N+TS V +  ++Y  I+GTY V+  P+HFACSD   C ++T 
Sbjct: 393 VLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITM 452

BLAST of Csor.00g136000 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 3.5e-39
Identity = 112/414 (27.05%), Postives = 174/414 (42.03%), Query Frame = 0

Query: 144 IPTPPKKGYKGSDSAIFNVLHFGAKGNGESDDTKAFEDAWVAACKVEGST-VMVPADYVF 203
           +P    +  +     + +V +FGAKGNG +DDTKAF DAW  AC  +  T ++VP +Y  
Sbjct: 37  LPRRQSRSTRPRSERLLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTC 96

Query: 204 FL-----------------DGTIMAPTDHRTW-GKGLLEWIQFTKLIGVTIRGSGIIDGR 263
            L                  GTI+AP D   W G    +W+ F  L  +T+ G G ++G 
Sbjct: 97  LLRPIDLSGPCKARLTLQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGM 156

Query: 264 GSVWWQDSSFDEPIDDEMKLIIPMNKTRDEAEPTPVGSNLEGKMPSIKPTALRFYGSFNV 323
           G  WW+ S                    + + P               PTAL F+   N+
Sbjct: 157 GQEWWERSC-----------------KHNHSNPCRGA-----------PTALTFHKCKNM 216

Query: 324 TVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPNTDGIQLQNSKDVLIHSTSLSCG 383
            V  + + +S Q H+   +C  V +  + V +P  SPNTDGI +  S+ ++I +T++S G
Sbjct: 217 RVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDGIHISVSRGIVIDNTTVSTG 276

Query: 384 ------------------------------------W-------------------VRLC 443
                                               W                   VR+ 
Sbjct: 277 DDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVRDITVDTAIISDTANGVRIK 336

Query: 444 S--------ERSLLSNIQMSEVQLPIVIDQFYCDKAK-CSNQTSGVALSGIDYEKIRGTY 474
           +         + +  NI+M+ V  PI+IDQ+YCD  K C+NQTS +++  I +  +RGT 
Sbjct: 337 TWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCANQTSAISIENISFVHVRGTS 396

BLAST of Csor.00g136000 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 2.1e-36
Identity = 103/370 (27.84%), Postives = 157/370 (42.43%), Query Frame = 0

Query: 159 IFNVLHFGAKGNGESDDTKAFEDAWVAACKVEGSTVMVPAD-----------------YV 218
           IFNV  +GA G+G+ D T+AF  AW AACK   + ++VP +                 + 
Sbjct: 58  IFNVEKYGAVGDGKHDCTEAFSTAWQAACKKPSAMLLVPGNKKFVVNNLFFNGPCQPHFT 117

Query: 219 FFLDGTIMAPTDHRTWGKGLLEWIQFTKLIGVTIRGSGIIDGRGSVWWQDSSFDEPIDDE 278
           F +DG I A  +  +W    + W+QF KL G T+ G G+IDG+G  WW           +
Sbjct: 118 FKVDGIIAAYQNPASWKNNRI-WLQFAKLTGFTLMGKGVIDGQGKQWWA---------GQ 177

Query: 279 MKLIIPMNKTRDEAEPTPVGSNLEGKMPSIKPTALRFYGSFNVTVTGITIQNSPQCHLKF 338
            K +       D                  +PTA++F  S  + + G+ + NSP+ HL F
Sbjct: 178 CKWVNGREICNDRD----------------RPTAIKFDFSTGLIIQGLKLMNSPEFHLVF 237

Query: 339 DNCVGVLVHDISVSSPGNSPNTDGIQLQNSKDVLIHSTSLSCG----------------- 398
            NC GV +  IS+++P +SPNTDGI +  SK+  +   ++  G                 
Sbjct: 238 GNCEGVKIIGISITAPRDSPNTDGIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIED 297

Query: 399 ------------------------WVRL----------------------CSERSLLSNI 447
                                   +V +                       +   +  N+
Sbjct: 298 LICGPGHGISIGSLGRENSRAEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENV 357

BLAST of Csor.00g136000 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 1.2e-34
Identity = 105/372 (28.23%), Postives = 152/372 (40.86%), Query Frame = 0

Query: 157 SAIFNVLHFGAKGNGESDDTKAFEDAWVAACKVEGSTVMVPADYVFF------------- 216
           + +FNV  +GA G+G+ D T+AF   W AACK   + ++VPA+  FF             
Sbjct: 56  ATVFNVEQYGAVGDGKHDSTEAFATTWNAACKKASAVLLVPANKKFFVNNLVFRGPCQPH 115

Query: 217 ----LDGTIMAPTDHRTWGKGLLEWIQFTKLIGVTIRGSGIIDGRGSVWWQDSSFDEPID 276
               +DGTI+A  D   W K    W+QF +L    + G+G+IDG+G  WW          
Sbjct: 116 LSFKVDGTIVAQPDPARW-KNSKIWLQFAQLTDFNLMGTGVIDGQGQQWWA--------- 175

Query: 277 DEMKLIIPMNKTRDEAEPTPVGSNLEGKMPSIKPTALRFYGSFNVTVTGITIQNSPQCHL 336
            + K++       D                  +PTA++   S +VTV  +T+ NSP+ HL
Sbjct: 176 GQCKVVNGRTVCNDRN----------------RPTAIKIDYSKSVTVKELTLMNSPEFHL 235

Query: 337 KFDNCVGVLVHDISVSSPGNSPNTDGIQLQNSKDVLIHS--------------------- 396
            F  C GV +  + + +P +SPNTDGI +  SK   I                       
Sbjct: 236 VFGECEGVKIQGLKIKAPRDSPNTDGIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITI 295

Query: 397 TSLSCGWVRLCSERSL------------------------------------------LS 447
             L CG     S  SL                                            
Sbjct: 296 KDLICGPGHGISIGSLGRDNSRAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYE 355

BLAST of Csor.00g136000 vs. ExPASy Swiss-Prot
Match: Q9FY19 (Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 9.7e-34
Identity = 104/370 (28.11%), Postives = 154/370 (41.62%), Query Frame = 0

Query: 159 IFNVLHFGAKGNGESDDTKAFEDAWVAACKVEGSTVMVPAD-----------------YV 218
           +FNV H+GA G+G+ D T AFE  W AAC    +  +VPA+                 + 
Sbjct: 59  VFNVEHYGAVGDGKHDSTDAFEKTWNAACNKLSAVFLVPANKKFVVNNLVFYGPCQPHFS 118

Query: 219 FFLDGTIMAPTDHRTWGKGLLEWIQFTKLIGVTIRGSGIIDGRGSVWWQDSSFDEPIDDE 278
           F +DGTI A  D   W K    W+ F +L    + G+G+IDG+G+ WW          D+
Sbjct: 119 FKVDGTIAAYPDPAKW-KNSKIWMHFARLTDFNLMGTGVIDGQGNRWW---------SDQ 178

Query: 279 MKLIIPMNKTRDEAEPTPVGSNLEGKMPSIKPTALRFYGSFNVTVTGITIQNSPQCHLKF 338
            K I       D+                 +PTA++   S +VTV  +T+ NSP+ HL F
Sbjct: 179 CKTINGRTVCNDKG----------------RPTAIKIDFSKSVTVKELTLTNSPEFHLVF 238

Query: 339 DNCVGVLVHDISVSSPGNSPNTDGIQLQNSKDVLIHSTS--------------------- 398
             C GV +  I + +P +SPNTDGI +  SK   I   +                     
Sbjct: 239 GECDGVKIQGIKIKAPRDSPNTDGIDIFASKRFEIEKCTIGTGDDCVAVGTGSSNITIKD 298

Query: 399 LSCG--------------------WVRLCSERSL----------------------LSNI 447
           L+CG                    +V L   + +                        N+
Sbjct: 299 LTCGPGHGMSIGSLGKGNSRSEVSFVHLDGAKFIDTQNGLRIKTWQGGSGLASHITYENV 358

BLAST of Csor.00g136000 vs. NCBI nr
Match: KAG6585162.1 (Polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1126 bits (2912), Expect = 0.0
Identity = 548/548 (100.00%), Postives = 548/548 (100.00%), Query Frame = 0

Query: 1   MHRNETIGSKIAKSLVDLEAERCCHYFAGFELLLSAGFACCDLVPLVGFRKQMYAWLCGV 60
           MHRNETIGSKIAKSLVDLEAERCCHYFAGFELLLSAGFACCDLVPLVGFRKQMYAWLCGV
Sbjct: 1   MHRNETIGSKIAKSLVDLEAERCCHYFAGFELLLSAGFACCDLVPLVGFRKQMYAWLCGV 60

Query: 61  VENGFKLKHSLQLEWMYSFYKGCTARRGKHWRQSRQTSASLSKKKGSHGGSKSHHVSGSN 120
           VENGFKLKHSLQLEWMYSFYKGCTARRGKHWRQSRQTSASLSKKKGSHGGSKSHHVSGSN
Sbjct: 61  VENGFKLKHSLQLEWMYSFYKGCTARRGKHWRQSRQTSASLSKKKGSHGGSKSHHVSGSN 120

Query: 121 PKAPPSHNASPSSPIPPKPKEEIIPTPPKKGYKGSDSAIFNVLHFGAKGNGESDDTKAFE 180
           PKAPPSHNASPSSPIPPKPKEEIIPTPPKKGYKGSDSAIFNVLHFGAKGNGESDDTKAFE
Sbjct: 121 PKAPPSHNASPSSPIPPKPKEEIIPTPPKKGYKGSDSAIFNVLHFGAKGNGESDDTKAFE 180

Query: 181 DAWVAACKVEGSTVMVPADYVFFLDGTIMAPTDHRTWGKGLLEWIQFTKLIGVTIRGSGI 240
           DAWVAACKVEGSTVMVPADYVFFLDGTIMAPTDHRTWGKGLLEWIQFTKLIGVTIRGSGI
Sbjct: 181 DAWVAACKVEGSTVMVPADYVFFLDGTIMAPTDHRTWGKGLLEWIQFTKLIGVTIRGSGI 240

Query: 241 IDGRGSVWWQDSSFDEPIDDEMKLIIPMNKTRDEAEPTPVGSNLEGKMPSIKPTALRFYG 300
           IDGRGSVWWQDSSFDEPIDDEMKLIIPMNKTRDEAEPTPVGSNLEGKMPSIKPTALRFYG
Sbjct: 241 IDGRGSVWWQDSSFDEPIDDEMKLIIPMNKTRDEAEPTPVGSNLEGKMPSIKPTALRFYG 300

Query: 301 SFNVTVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPNTDGIQLQNSKDVLIHSTS 360
           SFNVTVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPNTDGIQLQNSKDVLIHSTS
Sbjct: 301 SFNVTVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPNTDGIQLQNSKDVLIHSTS 360

Query: 361 LSCGWVRLCSERSLLSNIQMSEVQLPIVIDQFYCDKAKCSNQTSGVALSGIDYEKIRGTY 420
           LSCGWVRLCSERSLLSNIQMSEVQLPIVIDQFYCDKAKCSNQTSGVALSGIDYEKIRGTY
Sbjct: 361 LSCGWVRLCSERSLLSNIQMSEVQLPIVIDQFYCDKAKCSNQTSGVALSGIDYEKIRGTY 420

Query: 421 TVKPVHFACSDNQPCRDVTPTTIELKPLQERYHLYDPFCWQTFGELKTPTSPPIGCLQIG 480
           TVKPVHFACSDNQPCRDVTPTTIELKPLQERYHLYDPFCWQTFGELKTPTSPPIGCLQIG
Sbjct: 421 TVKPVHFACSDNQPCRDVTPTTIELKPLQERYHLYDPFCWQTFGELKTPTSPPIGCLQIG 480

Query: 481 KPSSNRVQVSGGHGCLKSMALIVMALVVGAAVMSQNIESSDWEELTAFCAALPALSADVS 540
           KPSSNRVQVSGGHGCLKSMALIVMALVVGAAVMSQNIESSDWEELTAFCAALPALSADVS
Sbjct: 481 KPSSNRVQVSGGHGCLKSMALIVMALVVGAAVMSQNIESSDWEELTAFCAALPALSADVS 540

Query: 541 YFDTFPLR 548
           YFDTFPLR
Sbjct: 541 YFDTFPLR 548

BLAST of Csor.00g136000 vs. NCBI nr
Match: XP_022951189.1 (polygalacturonase At1g48100-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 833 bits (2152), Expect = 2.90e-300
Identity = 437/569 (76.80%), Postives = 446/569 (78.38%), Query Frame = 0

Query: 1   MHRNETIGSKIAKSLVDLEAERCCHYFAGFELLLSAGFACCDLVPLVGFRKQMYAWLCGV 60
           MHRNETIGSKIAKSLVDLEA RCCHYFAGF+LLLSAGFACCDLVPLV             
Sbjct: 1   MHRNETIGSKIAKSLVDLEARRCCHYFAGFKLLLSAGFACCDLVPLV------------- 60

Query: 61  VENGFKLKHSLQLEWMYSFYKGCTARRGKHWRQSRQTSASLSKKKGSHGGSKSHHVSGSN 120
           V+                   GCTARRGKHWRQSRQTSASLSKKKGSHGGSKSHHVSGSN
Sbjct: 61  VQEA----------------NGCTARRGKHWRQSRQTSASLSKKKGSHGGSKSHHVSGSN 120

Query: 121 PKAPPSHNASPSSPIPPKPKEEIIPTPPKKGYKGSDSAIFNVLHFGAKGNGESDDTKAFE 180
           PKAPPSHNASPSSPIPPKPKEEIIPTPPKKGYKGSDS IFNVL FGAKGNGESDDTKAFE
Sbjct: 121 PKAPPSHNASPSSPIPPKPKEEIIPTPPKKGYKGSDSVIFNVLDFGAKGNGESDDTKAFE 180

Query: 181 DAWVAACKVEGSTVMVPADYVFF------------------LDGTIMAPTDHRTWGKGLL 240
           DAWVAACKVEGSTVMVPADYVFF                  LDGTIMAPTDHRTWGKGLL
Sbjct: 181 DAWVAACKVEGSTVMVPADYVFFVGPISFSGPYCQPDIVFQLDGTIMAPTDHRTWGKGLL 240

Query: 241 EWIQFTKLIGVTIRGSGIIDGRGSVWWQDSSFDEPIDDEMKLIIPMNKTRDEAEPTPVGS 300
           +WIQFTKLIGVTIRGSGIIDGRGSVWWQDSSFDEPIDDEMKLIIPMNKTRDEA+PTPVGS
Sbjct: 241 QWIQFTKLIGVTIRGSGIIDGRGSVWWQDSSFDEPIDDEMKLIIPMNKTRDEAKPTPVGS 300

Query: 301 NLEGKMPSIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPN 360
           NLEGK+PSIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPN
Sbjct: 301 NLEGKIPSIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPN 360

Query: 361 TDGIQLQNSKDVLIHSTSLSCG--------------------W----------------- 420
           TDGI LQNSKDVLIHSTSLSCG                    W                 
Sbjct: 361 TDGIHLQNSKDVLIHSTSLSCGDDCVSIQTGCSNIYIHNVNCWPGHGISIGSLGKDNTKA 420

Query: 421 ------------------VRLCS--------ERSLLSNIQMSEVQLPIVIDQFYCDKAKC 480
                             VR+ +        +  L SNIQMSEVQLPIVIDQFYCDKAKC
Sbjct: 421 CVSNITVRDVTMHNTMNGVRIKTWQGGSGSVQNVLFSNIQMSEVQLPIVIDQFYCDKAKC 480

Query: 481 SNQTSGVALSGIDYEKIRGTYTVKPVHFACSDNQPCRDVTPTTIELKPLQERYHLYDPFC 488
           SNQTSGVA SGIDYEKIRGTYTVKPVHFACSDNQPCRDVT TTIELKPLQERYHL+DPFC
Sbjct: 481 SNQTSGVAQSGIDYEKIRGTYTVKPVHFACSDNQPCRDVTLTTIELKPLQERYHLHDPFC 540

BLAST of Csor.00g136000 vs. NCBI nr
Match: XP_022951190.1 (polygalacturonase At1g48100-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 758 bits (1958), Expect = 3.12e-271
Identity = 394/497 (79.28%), Postives = 403/497 (81.09%), Query Frame = 0

Query: 73  LEWMYSFYKGCTARRGKHWRQSRQTSASLSKKKGSHGGSKSHHVSGSNPKAPPSHNASPS 132
           L W  +F +GCTARRGKHWRQSRQTSASLSKKKGSHGGSKSHHVSGSNPKAPPSHNASPS
Sbjct: 15  LVWSSNF-EGCTARRGKHWRQSRQTSASLSKKKGSHGGSKSHHVSGSNPKAPPSHNASPS 74

Query: 133 SPIPPKPKEEIIPTPPKKGYKGSDSAIFNVLHFGAKGNGESDDTKAFEDAWVAACKVEGS 192
           SPIPPKPKEEIIPTPPKKGYKGSDS IFNVL FGAKGNGESDDTKAFEDAWVAACKVEGS
Sbjct: 75  SPIPPKPKEEIIPTPPKKGYKGSDSVIFNVLDFGAKGNGESDDTKAFEDAWVAACKVEGS 134

Query: 193 TVMVPADYVFF------------------LDGTIMAPTDHRTWGKGLLEWIQFTKLIGVT 252
           TVMVPADYVFF                  LDGTIMAPTDHRTWGKGLL+WIQFTKLIGVT
Sbjct: 135 TVMVPADYVFFVGPISFSGPYCQPDIVFQLDGTIMAPTDHRTWGKGLLQWIQFTKLIGVT 194

Query: 253 IRGSGIIDGRGSVWWQDSSFDEPIDDEMKLIIPMNKTRDEAEPTPVGSNLEGKMPSIKPT 312
           IRGSGIIDGRGSVWWQDSSFDEPIDDEMKLIIPMNKTRDEA+PTPVGSNLEGK+PSIKPT
Sbjct: 195 IRGSGIIDGRGSVWWQDSSFDEPIDDEMKLIIPMNKTRDEAKPTPVGSNLEGKIPSIKPT 254

Query: 313 ALRFYGSFNVTVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPNTDGIQLQNSKDV 372
           ALRFYGSFNVTVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPNTDGI LQNSKDV
Sbjct: 255 ALRFYGSFNVTVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPNTDGIHLQNSKDV 314

Query: 373 LIHSTSLSCG--------------------W----------------------------- 432
           LIHSTSLSCG                    W                             
Sbjct: 315 LIHSTSLSCGDDCVSIQTGCSNIYIHNVNCWPGHGISIGSLGKDNTKACVSNITVRDVTM 374

Query: 433 ------VRLCS--------ERSLLSNIQMSEVQLPIVIDQFYCDKAKCSNQTSGVALSGI 488
                 VR+ +        +  L SNIQMSEVQLPIVIDQFYCDKAKCSNQTSGVA SGI
Sbjct: 375 HNTMNGVRIKTWQGGSGSVQNVLFSNIQMSEVQLPIVIDQFYCDKAKCSNQTSGVAQSGI 434

BLAST of Csor.00g136000 vs. NCBI nr
Match: XP_022951187.1 (polygalacturonase At1g48100-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 757 bits (1955), Expect = 5.00e-270
Identity = 391/489 (79.96%), Postives = 399/489 (81.60%), Query Frame = 0

Query: 81  KGCTARRGKHWRQSRQTSASLSKKKGSHGGSKSHHVSGSNPKAPPSHNASPSSPIPPKPK 140
           +GCTARRGKHWRQSRQTSASLSKKKGSHGGSKSHHVSGSNPKAPPSHNASPSSPIPPKPK
Sbjct: 69  RGCTARRGKHWRQSRQTSASLSKKKGSHGGSKSHHVSGSNPKAPPSHNASPSSPIPPKPK 128

Query: 141 EEIIPTPPKKGYKGSDSAIFNVLHFGAKGNGESDDTKAFEDAWVAACKVEGSTVMVPADY 200
           EEIIPTPPKKGYKGSDS IFNVL FGAKGNGESDDTKAFEDAWVAACKVEGSTVMVPADY
Sbjct: 129 EEIIPTPPKKGYKGSDSVIFNVLDFGAKGNGESDDTKAFEDAWVAACKVEGSTVMVPADY 188

Query: 201 VFF------------------LDGTIMAPTDHRTWGKGLLEWIQFTKLIGVTIRGSGIID 260
           VFF                  LDGTIMAPTDHRTWGKGLL+WIQFTKLIGVTIRGSGIID
Sbjct: 189 VFFVGPISFSGPYCQPDIVFQLDGTIMAPTDHRTWGKGLLQWIQFTKLIGVTIRGSGIID 248

Query: 261 GRGSVWWQDSSFDEPIDDEMKLIIPMNKTRDEAEPTPVGSNLEGKMPSIKPTALRFYGSF 320
           GRGSVWWQDSSFDEPIDDEMKLIIPMNKTRDEA+PTPVGSNLEGK+PSIKPTALRFYGSF
Sbjct: 249 GRGSVWWQDSSFDEPIDDEMKLIIPMNKTRDEAKPTPVGSNLEGKIPSIKPTALRFYGSF 308

Query: 321 NVTVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPNTDGIQLQNSKDVLIHSTSLS 380
           NVTVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPNTDGI LQNSKDVLIHSTSLS
Sbjct: 309 NVTVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPNTDGIHLQNSKDVLIHSTSLS 368

Query: 381 CG--------------------W-----------------------------------VR 440
           CG                    W                                   VR
Sbjct: 369 CGDDCVSIQTGCSNIYIHNVNCWPGHGISIGSLGKDNTKACVSNITVRDVTMHNTMNGVR 428

Query: 441 LCS--------ERSLLSNIQMSEVQLPIVIDQFYCDKAKCSNQTSGVALSGIDYEKIRGT 488
           + +        +  L SNIQMSEVQLPIVIDQFYCDKAKCSNQTSGVA SGIDYEKIRGT
Sbjct: 429 IKTWQGGSGSVQNVLFSNIQMSEVQLPIVIDQFYCDKAKCSNQTSGVAQSGIDYEKIRGT 488

BLAST of Csor.00g136000 vs. NCBI nr
Match: XP_023537364.1 (polygalacturonase At1g48100-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023537365.1 polygalacturonase At1g48100-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023537366.1 polygalacturonase At1g48100-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 755 bits (1949), Expect = 7.30e-270
Identity = 393/504 (77.98%), Postives = 403/504 (79.96%), Query Frame = 0

Query: 73  LEWMYSFYKGCTARRGKHWRQSRQTSASLSKKKGSHGGSKSHHVSGSNPKAPPSHNASPS 132
           L W  +F +GCTARRGKHWRQSRQTSASLSKKKGSHGGSKSHHVSGSNPKAPPSHNASPS
Sbjct: 15  LVWSSNF-EGCTARRGKHWRQSRQTSASLSKKKGSHGGSKSHHVSGSNPKAPPSHNASPS 74

Query: 133 SPIPPKPKEEIIPTPPKKGYKGSDSAIFNVLHFGAKGNGESDDTKAFEDAWVAACKVEGS 192
           SPIPPKPKEEIIPTPPKKGYKGSDS+IFNVL FGAKGNGESDDTKAFEDAWVAACKVEGS
Sbjct: 75  SPIPPKPKEEIIPTPPKKGYKGSDSSIFNVLDFGAKGNGESDDTKAFEDAWVAACKVEGS 134

Query: 193 TVMVPADYVFF------------------LDGTIMAPTDHRTWGKGLLEWIQFTKLIGVT 252
           TVMVPADYVFF                  LDGTI+APTDHRTWGKGLL+WIQFTKLIGVT
Sbjct: 135 TVMVPADYVFFVGPISFSGPYYQPDIVFQLDGTIIAPTDHRTWGKGLLQWIQFTKLIGVT 194

Query: 253 IRGSGIIDGRGSVWWQDSSFDEPIDDEMKLIIPMNKTRDEAEPTPVGSNLEGKMPSIKPT 312
           IRGSGIIDGRGSVWWQDSSFDEPIDDEMKLIIPMNKTRDEA+PTPVGSNLEGKMPSIKPT
Sbjct: 195 IRGSGIIDGRGSVWWQDSSFDEPIDDEMKLIIPMNKTRDEAKPTPVGSNLEGKMPSIKPT 254

Query: 313 ALRFYGSFNVTVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPNTDGIQLQNSKDV 372
           ALRFYGSFNVTVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPNTD I LQNSKDV
Sbjct: 255 ALRFYGSFNVTVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPNTDVIHLQNSKDV 314

Query: 373 LIHSTSLSCG-------------------------------------------------- 432
           LIHSTSLSCG                                                  
Sbjct: 315 LIHSTSLSCGDDCVSIQTGCSNIYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVTM 374

Query: 433 -----WVRLCS--------ERSLLSNIQMSEVQLPIVIDQFYCDKAKCSNQTSGVALSGI 492
                 VR+ +        +  L SNIQMSEVQLPIVIDQFYCDKAKCSNQTSGVALSGI
Sbjct: 375 HNTMNGVRIKTWQGGSGSVQNVLFSNIQMSEVQLPIVIDQFYCDKAKCSNQTSGVALSGI 434

Query: 493 DYEKIRGTYTVKPVHFACSDNQPCRDVTPTTIELKPLQERYHLYDPFCWQTFGELKTPTS 495
           +YEKIRGTYTVKPVHFACSDNQPCRDVT TTIELKPLQERYHLYDPF WQTFGELKTPTS
Sbjct: 435 NYEKIRGTYTVKPVHFACSDNQPCRDVTLTTIELKPLQERYHLYDPFWWQTFGELKTPTS 494

BLAST of Csor.00g136000 vs. ExPASy TrEMBL
Match: A0A6J1GI03 (polygalacturonase At1g48100-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111454109 PE=3 SV=1)

HSP 1 Score: 833 bits (2152), Expect = 1.40e-300
Identity = 437/569 (76.80%), Postives = 446/569 (78.38%), Query Frame = 0

Query: 1   MHRNETIGSKIAKSLVDLEAERCCHYFAGFELLLSAGFACCDLVPLVGFRKQMYAWLCGV 60
           MHRNETIGSKIAKSLVDLEA RCCHYFAGF+LLLSAGFACCDLVPLV             
Sbjct: 1   MHRNETIGSKIAKSLVDLEARRCCHYFAGFKLLLSAGFACCDLVPLV------------- 60

Query: 61  VENGFKLKHSLQLEWMYSFYKGCTARRGKHWRQSRQTSASLSKKKGSHGGSKSHHVSGSN 120
           V+                   GCTARRGKHWRQSRQTSASLSKKKGSHGGSKSHHVSGSN
Sbjct: 61  VQEA----------------NGCTARRGKHWRQSRQTSASLSKKKGSHGGSKSHHVSGSN 120

Query: 121 PKAPPSHNASPSSPIPPKPKEEIIPTPPKKGYKGSDSAIFNVLHFGAKGNGESDDTKAFE 180
           PKAPPSHNASPSSPIPPKPKEEIIPTPPKKGYKGSDS IFNVL FGAKGNGESDDTKAFE
Sbjct: 121 PKAPPSHNASPSSPIPPKPKEEIIPTPPKKGYKGSDSVIFNVLDFGAKGNGESDDTKAFE 180

Query: 181 DAWVAACKVEGSTVMVPADYVFF------------------LDGTIMAPTDHRTWGKGLL 240
           DAWVAACKVEGSTVMVPADYVFF                  LDGTIMAPTDHRTWGKGLL
Sbjct: 181 DAWVAACKVEGSTVMVPADYVFFVGPISFSGPYCQPDIVFQLDGTIMAPTDHRTWGKGLL 240

Query: 241 EWIQFTKLIGVTIRGSGIIDGRGSVWWQDSSFDEPIDDEMKLIIPMNKTRDEAEPTPVGS 300
           +WIQFTKLIGVTIRGSGIIDGRGSVWWQDSSFDEPIDDEMKLIIPMNKTRDEA+PTPVGS
Sbjct: 241 QWIQFTKLIGVTIRGSGIIDGRGSVWWQDSSFDEPIDDEMKLIIPMNKTRDEAKPTPVGS 300

Query: 301 NLEGKMPSIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPN 360
           NLEGK+PSIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPN
Sbjct: 301 NLEGKIPSIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPN 360

Query: 361 TDGIQLQNSKDVLIHSTSLSCG--------------------W----------------- 420
           TDGI LQNSKDVLIHSTSLSCG                    W                 
Sbjct: 361 TDGIHLQNSKDVLIHSTSLSCGDDCVSIQTGCSNIYIHNVNCWPGHGISIGSLGKDNTKA 420

Query: 421 ------------------VRLCS--------ERSLLSNIQMSEVQLPIVIDQFYCDKAKC 480
                             VR+ +        +  L SNIQMSEVQLPIVIDQFYCDKAKC
Sbjct: 421 CVSNITVRDVTMHNTMNGVRIKTWQGGSGSVQNVLFSNIQMSEVQLPIVIDQFYCDKAKC 480

Query: 481 SNQTSGVALSGIDYEKIRGTYTVKPVHFACSDNQPCRDVTPTTIELKPLQERYHLYDPFC 488
           SNQTSGVA SGIDYEKIRGTYTVKPVHFACSDNQPCRDVT TTIELKPLQERYHL+DPFC
Sbjct: 481 SNQTSGVAQSGIDYEKIRGTYTVKPVHFACSDNQPCRDVTLTTIELKPLQERYHLHDPFC 540

BLAST of Csor.00g136000 vs. ExPASy TrEMBL
Match: A0A6J1GI00 (polygalacturonase At1g48100-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111454109 PE=3 SV=1)

HSP 1 Score: 758 bits (1958), Expect = 1.51e-271
Identity = 394/497 (79.28%), Postives = 403/497 (81.09%), Query Frame = 0

Query: 73  LEWMYSFYKGCTARRGKHWRQSRQTSASLSKKKGSHGGSKSHHVSGSNPKAPPSHNASPS 132
           L W  +F +GCTARRGKHWRQSRQTSASLSKKKGSHGGSKSHHVSGSNPKAPPSHNASPS
Sbjct: 15  LVWSSNF-EGCTARRGKHWRQSRQTSASLSKKKGSHGGSKSHHVSGSNPKAPPSHNASPS 74

Query: 133 SPIPPKPKEEIIPTPPKKGYKGSDSAIFNVLHFGAKGNGESDDTKAFEDAWVAACKVEGS 192
           SPIPPKPKEEIIPTPPKKGYKGSDS IFNVL FGAKGNGESDDTKAFEDAWVAACKVEGS
Sbjct: 75  SPIPPKPKEEIIPTPPKKGYKGSDSVIFNVLDFGAKGNGESDDTKAFEDAWVAACKVEGS 134

Query: 193 TVMVPADYVFF------------------LDGTIMAPTDHRTWGKGLLEWIQFTKLIGVT 252
           TVMVPADYVFF                  LDGTIMAPTDHRTWGKGLL+WIQFTKLIGVT
Sbjct: 135 TVMVPADYVFFVGPISFSGPYCQPDIVFQLDGTIMAPTDHRTWGKGLLQWIQFTKLIGVT 194

Query: 253 IRGSGIIDGRGSVWWQDSSFDEPIDDEMKLIIPMNKTRDEAEPTPVGSNLEGKMPSIKPT 312
           IRGSGIIDGRGSVWWQDSSFDEPIDDEMKLIIPMNKTRDEA+PTPVGSNLEGK+PSIKPT
Sbjct: 195 IRGSGIIDGRGSVWWQDSSFDEPIDDEMKLIIPMNKTRDEAKPTPVGSNLEGKIPSIKPT 254

Query: 313 ALRFYGSFNVTVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPNTDGIQLQNSKDV 372
           ALRFYGSFNVTVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPNTDGI LQNSKDV
Sbjct: 255 ALRFYGSFNVTVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPNTDGIHLQNSKDV 314

Query: 373 LIHSTSLSCG--------------------W----------------------------- 432
           LIHSTSLSCG                    W                             
Sbjct: 315 LIHSTSLSCGDDCVSIQTGCSNIYIHNVNCWPGHGISIGSLGKDNTKACVSNITVRDVTM 374

Query: 433 ------VRLCS--------ERSLLSNIQMSEVQLPIVIDQFYCDKAKCSNQTSGVALSGI 488
                 VR+ +        +  L SNIQMSEVQLPIVIDQFYCDKAKCSNQTSGVA SGI
Sbjct: 375 HNTMNGVRIKTWQGGSGSVQNVLFSNIQMSEVQLPIVIDQFYCDKAKCSNQTSGVAQSGI 434

BLAST of Csor.00g136000 vs. ExPASy TrEMBL
Match: A0A6J1GGZ4 (polygalacturonase At1g48100-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454109 PE=3 SV=1)

HSP 1 Score: 757 bits (1955), Expect = 2.42e-270
Identity = 391/489 (79.96%), Postives = 399/489 (81.60%), Query Frame = 0

Query: 81  KGCTARRGKHWRQSRQTSASLSKKKGSHGGSKSHHVSGSNPKAPPSHNASPSSPIPPKPK 140
           +GCTARRGKHWRQSRQTSASLSKKKGSHGGSKSHHVSGSNPKAPPSHNASPSSPIPPKPK
Sbjct: 69  RGCTARRGKHWRQSRQTSASLSKKKGSHGGSKSHHVSGSNPKAPPSHNASPSSPIPPKPK 128

Query: 141 EEIIPTPPKKGYKGSDSAIFNVLHFGAKGNGESDDTKAFEDAWVAACKVEGSTVMVPADY 200
           EEIIPTPPKKGYKGSDS IFNVL FGAKGNGESDDTKAFEDAWVAACKVEGSTVMVPADY
Sbjct: 129 EEIIPTPPKKGYKGSDSVIFNVLDFGAKGNGESDDTKAFEDAWVAACKVEGSTVMVPADY 188

Query: 201 VFF------------------LDGTIMAPTDHRTWGKGLLEWIQFTKLIGVTIRGSGIID 260
           VFF                  LDGTIMAPTDHRTWGKGLL+WIQFTKLIGVTIRGSGIID
Sbjct: 189 VFFVGPISFSGPYCQPDIVFQLDGTIMAPTDHRTWGKGLLQWIQFTKLIGVTIRGSGIID 248

Query: 261 GRGSVWWQDSSFDEPIDDEMKLIIPMNKTRDEAEPTPVGSNLEGKMPSIKPTALRFYGSF 320
           GRGSVWWQDSSFDEPIDDEMKLIIPMNKTRDEA+PTPVGSNLEGK+PSIKPTALRFYGSF
Sbjct: 249 GRGSVWWQDSSFDEPIDDEMKLIIPMNKTRDEAKPTPVGSNLEGKIPSIKPTALRFYGSF 308

Query: 321 NVTVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPNTDGIQLQNSKDVLIHSTSLS 380
           NVTVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPNTDGI LQNSKDVLIHSTSLS
Sbjct: 309 NVTVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPNTDGIHLQNSKDVLIHSTSLS 368

Query: 381 CG--------------------W-----------------------------------VR 440
           CG                    W                                   VR
Sbjct: 369 CGDDCVSIQTGCSNIYIHNVNCWPGHGISIGSLGKDNTKACVSNITVRDVTMHNTMNGVR 428

Query: 441 LCS--------ERSLLSNIQMSEVQLPIVIDQFYCDKAKCSNQTSGVALSGIDYEKIRGT 488
           + +        +  L SNIQMSEVQLPIVIDQFYCDKAKCSNQTSGVA SGIDYEKIRGT
Sbjct: 429 IKTWQGGSGSVQNVLFSNIQMSEVQLPIVIDQFYCDKAKCSNQTSGVAQSGIDYEKIRGT 488

BLAST of Csor.00g136000 vs. ExPASy TrEMBL
Match: A0A6J1HAQ2 (polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111462351 PE=3 SV=1)

HSP 1 Score: 696 bits (1796), Expect = 7.45e-247
Identity = 358/504 (71.03%), Postives = 382/504 (75.79%), Query Frame = 0

Query: 73  LEWMYSFYKGCTARRGKHWRQSRQTSASLSKKKGSHGGSKSHHVSGSNPKAPPSHNASPS 132
           L W  +F + C ARRGKHWRQSR  SASLSKKKGSHGGSKSHH  GS+ KAPP HNASP 
Sbjct: 19  LVWSSNF-EACIARRGKHWRQSRDASASLSKKKGSHGGSKSHHSGGSHSKAPPPHNASPP 78

Query: 133 SPIPPKPKEEIIPTPPKKGYKGSDSAIFNVLHFGAKGNGESDDTKAFEDAWVAACKVEGS 192
           SPIPPKPKEEI+ TPPKKGY G DSAIFNVL FGAKGNGE+DDTKAFEDAW AACKVEGS
Sbjct: 79  SPIPPKPKEEIVQTPPKKGYNGGDSAIFNVLDFGAKGNGETDDTKAFEDAWAAACKVEGS 138

Query: 193 TVMVPADYVFF------------------LDGTIMAPTDHRTWGKGLLEWIQFTKLIGVT 252
           TVMVPA+YVFF                  LDGTI+APTD +TWGKGLL+WIQFTKL+G+T
Sbjct: 139 TVMVPAEYVFFVGPISFSGPYCQPDIVFQLDGTIIAPTDRQTWGKGLLQWIQFTKLVGIT 198

Query: 253 IRGSGIIDGRGSVWWQDSSFDEPIDDEMKLIIPMNKTRDEAEPTPVGSNLEGKMPSIKPT 312
           IRGSG+IDGRGSVWWQDSSFDEPIDDEMKLIIP+N TR E +PTP+GSNL+GKMPSIKPT
Sbjct: 199 IRGSGVIDGRGSVWWQDSSFDEPIDDEMKLIIPINNTRAETKPTPIGSNLDGKMPSIKPT 258

Query: 313 ALRFYGSFNVTVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPNTDGIQLQNSKDV 372
           ALRFYGSFN TVTGITIQNSPQCHLKFDNCVGVLVHD SVSSPG+S NTDGI LQNSKDV
Sbjct: 259 ALRFYGSFNATVTGITIQNSPQCHLKFDNCVGVLVHDFSVSSPGDSLNTDGIHLQNSKDV 318

Query: 373 LIHSTSLSCG-------------------------------------------------- 432
           LIHS+SLSCG                                                  
Sbjct: 319 LIHSSSLSCGDDCVSIQTGCSNVYIHNVNCGPGHGISIGSLGKDHTKACVSNITVRDVTM 378

Query: 433 -----WVRLCS--------ERSLLSNIQMSEVQLPIVIDQFYCDKAKCSNQTSGVALSGI 492
                 VR+ +        +  L SNIQMSEVQLPIVIDQFYCDKAKC+NQTS VALSGI
Sbjct: 379 HNTMNGVRIKTWQGGSGSVQNVLFSNIQMSEVQLPIVIDQFYCDKAKCTNQTSAVALSGI 438

Query: 493 DYEKIRGTYTVKPVHFACSDNQPCRDVTPTTIELKPLQERYHLYDPFCWQTFGELKTPTS 495
           +YEKIRGTYTVKPVHFACSDN PC DVT TTIELKPLQERYHLYDPFCWQTFGEL+TPTS
Sbjct: 439 NYEKIRGTYTVKPVHFACSDNLPCTDVTLTTIELKPLQERYHLYDPFCWQTFGELRTPTS 498

BLAST of Csor.00g136000 vs. ExPASy TrEMBL
Match: A0A6J1K7Y9 (polygalacturonase At1g48100-like OS=Cucurbita maxima OX=3661 GN=LOC111492497 PE=3 SV=1)

HSP 1 Score: 694 bits (1790), Expect = 6.08e-246
Identity = 357/504 (70.83%), Postives = 382/504 (75.79%), Query Frame = 0

Query: 73  LEWMYSFYKGCTARRGKHWRQSRQTSASLSKKKGSHGGSKSHHVSGSNPKAPPSHNASPS 132
           L W  +F + C ARRGKHWRQSR+ SASLSKKKGSHGGSKSHH  G + KAPP HNASP 
Sbjct: 19  LVWSSNF-EACIARRGKHWRQSREASASLSKKKGSHGGSKSHHSGGFHSKAPPPHNASPP 78

Query: 133 SPIPPKPKEEIIPTPPKKGYKGSDSAIFNVLHFGAKGNGESDDTKAFEDAWVAACKVEGS 192
           SPIPPKPKEEI+ TPPKKGY G DSAIFNVL FGAKGNGE+DDTKAFEDAW AACKVEGS
Sbjct: 79  SPIPPKPKEEIVQTPPKKGYNGGDSAIFNVLDFGAKGNGETDDTKAFEDAWAAACKVEGS 138

Query: 193 TVMVPADYVFF------------------LDGTIMAPTDHRTWGKGLLEWIQFTKLIGVT 252
           TVMVPA+YVF+                  LDGTI+APTD +TWGKGLL+WIQFTKL+G+T
Sbjct: 139 TVMVPAEYVFYVGPISFSGPYCQPNIVFQLDGTIIAPTDRQTWGKGLLQWIQFTKLVGIT 198

Query: 253 IRGSGIIDGRGSVWWQDSSFDEPIDDEMKLIIPMNKTRDEAEPTPVGSNLEGKMPSIKPT 312
           IRGSGIIDGRGSVWWQDSSFDEPIDDEMKLIIP+N TR E +PTP+GSNL+GKMPSIKPT
Sbjct: 199 IRGSGIIDGRGSVWWQDSSFDEPIDDEMKLIIPINNTRAETKPTPIGSNLDGKMPSIKPT 258

Query: 313 ALRFYGSFNVTVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPNTDGIQLQNSKDV 372
           ALRFYGSFN TVTGITIQNSPQCHLKFDNCVGVLVHD SVSSPG+S NTDGI LQNSKDV
Sbjct: 259 ALRFYGSFNATVTGITIQNSPQCHLKFDNCVGVLVHDFSVSSPGDSLNTDGIHLQNSKDV 318

Query: 373 LIHSTSLSCG-------------------------------------------------- 432
           LIHS+SLSCG                                                  
Sbjct: 319 LIHSSSLSCGDDCVSIQTGCSNVYIHNVNCGPGHGISIGSLGKDHTKACVSNITVRDVTM 378

Query: 433 -----WVRLCS--------ERSLLSNIQMSEVQLPIVIDQFYCDKAKCSNQTSGVALSGI 492
                 VR+ +        +  L SNIQMSEVQLPIVIDQFYCDKAKC+NQTS VALSGI
Sbjct: 379 HNTMNGVRIKTWQGGSGSVQNVLFSNIQMSEVQLPIVIDQFYCDKAKCTNQTSAVALSGI 438

Query: 493 DYEKIRGTYTVKPVHFACSDNQPCRDVTPTTIELKPLQERYHLYDPFCWQTFGELKTPTS 495
           +YEKIRGTYTVKPVHFACSDN PC DVT TTIELKPLQERYHLYDPFCWQTFGEL+TPTS
Sbjct: 439 NYEKIRGTYTVKPVHFACSDNLPCTDVTLTTIELKPLQERYHLYDPFCWQTFGELRTPTS 498

BLAST of Csor.00g136000 vs. TAIR 10
Match: AT1G10640.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 475.7 bits (1223), Expect = 5.0e-134
Identity = 257/505 (50.89%), Postives = 319/505 (63.17%), Query Frame = 0

Query: 83  CTARRGKHWRQSRQTSASLS-----KKKGSHGGS--------KSHHVSGSNP----KAPP 142
           C ARRG+HWR S ++S+ LS     KK  SHG S          HH S   P    K PP
Sbjct: 30  CIARRGRHWRHSHRSSSDLSDSLSNKKPKSHGNSHHHSSHNNNHHHKSKPKPKPKLKTPP 89

Query: 143 SHNASPSSPIPPKPKEEIIPTPPKKGYKGSDSAIFNVLHFGAKGNGESDDTKAFEDAWVA 202
             + + S  +   PK +  P PP+KG     S +FNV+ FGAKG+G+ DDTKAFE AWVA
Sbjct: 90  KAHDNNSPVVSGPPKVQPPPLPPQKG-----SQVFNVMDFGAKGDGKCDDTKAFEAAWVA 149

Query: 203 ACKVEGSTVMVPADY------------------VFFLDGTIMAPTDHRTWGKGLLEWIQF 262
           ACK+E S ++VP +Y                  VF LDGTI+APTD +TWGKGL+ WI F
Sbjct: 150 ACKIEASVMLVPPEYTYLVGPISFSGPYCQANIVFQLDGTIIAPTDSKTWGKGLMWWIDF 209

Query: 263 TKLIGVTIRGSGIIDGRGSVWW-QDSSFDEPIDDEMKLIIPMNKTRDEAEPTPVGSNLEG 322
           TKL G+ ++G G+IDGRGS WW QDS F   ID + KLI+P+N + ++  P P+ S L+ 
Sbjct: 210 TKLKGIKVQGKGVIDGRGSGWWQQDSPF---IDSDTKLIVPLNNSANQNPPMPIRSELDE 269

Query: 323 KMPSIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPNTDGI 382
           +MPSIKPTALRF GSF V VTGITIQNSPQCHLKFD+CVGV+VHDI+VSSPG+SPNTDGI
Sbjct: 270 RMPSIKPTALRFSGSFGVEVTGITIQNSPQCHLKFDDCVGVVVHDIAVSSPGDSPNTDGI 329

Query: 383 QLQNSKDVLIHSTSLSCG------------------------------------------ 442
            LQN+KDVLIHST+L+CG                                          
Sbjct: 330 HLQNTKDVLIHSTTLACGDDCISIQTGCSNVFVHNVNCGPGHGISIGSLGKEGTKACVSN 389

Query: 443 -------------WVRLCSERS--------LLSNIQMSEVQLPIVIDQFYCDKAKCSNQT 489
                         VR+ + +         + SNIQ+++VQ+PI I+QFYCD +KC NQT
Sbjct: 390 ITVRDVAMHNTMTGVRIKTWQGGVGSVKGIIFSNIQLNQVQIPITINQFYCDHSKCKNQT 449

BLAST of Csor.00g136000 vs. TAIR 10
Match: AT1G60590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 468.8 bits (1205), Expect = 6.1e-132
Identity = 256/503 (50.89%), Postives = 315/503 (62.62%), Query Frame = 0

Query: 83  CTARRGKHWRQSRQTSASL-----SKKKGSHGGSKSHHVSGSNPK------APPSHNASP 142
           C ARRG+HWR   ++S+SL     SKK  SH  +  H    S PK       P S++ SP
Sbjct: 30  CIARRGRHWRHHHRSSSSLSDSLSSKKPKSHENNHHHMSHKSKPKPKMKTQPPKSNDGSP 89

Query: 143 SSPIPPK----PKEEIIPTP-PKKGYKGSDSAIFNVLHFGAKGNGESDDTKAFEDAWVAA 202
               PP+    P   + P P P       DS  FNVL FGAKG+G SDDT+AFE AW +A
Sbjct: 90  VVSQPPQVQQPPPPHVQPPPTPLPLQPVEDSQQFNVLDFGAKGDGMSDDTQAFEAAWASA 149

Query: 203 CKVEGSTVMVPADYVFF------------------LDGTIMAPTDHRTWGKGLLEWIQFT 262
           CKVE ST+++P DY+F                   L+G I+APTD  +WG GL+ WI+FT
Sbjct: 150 CKVEASTMIIPPDYIFLVGPISFSGPYCQANIVFQLEGMIVAPTDTESWGGGLMWWIEFT 209

Query: 263 KLIGVTIRGSGIIDGRGSVWW-QDSSFDEPIDDEMKLIIPMNKTRDEAEPTPVGSNLEGK 322
           KL G+TI+G+G+IDGRG+VWW QD   D PIDD+ KLI+P+N +  E  P P+ S L  +
Sbjct: 210 KLSGITIQGNGVIDGRGTVWWQQDYLSDYPIDDDFKLIVPLNNSVQERPPMPIRSELNWR 269

Query: 323 MPSIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCVGVLVHDISVSSPGNSPNTDGIQ 382
           MPSIKPTALRFYGS +VTVTGITIQNSPQCHLKFDNCV VLVHD++VSSPG+SPNTDGI 
Sbjct: 270 MPSIKPTALRFYGSIDVTVTGITIQNSPQCHLKFDNCVKVLVHDVNVSSPGDSPNTDGIH 329

Query: 383 LQNSKDVLIHSTSLSCG------------------------------------------- 442
           LQN++DV+IH+T+L+CG                                           
Sbjct: 330 LQNTRDVMIHTTTLACGDDCISIQTGCSNVYVHNVNCGPGHGISIGSLGKDSTKACVSNI 389

Query: 443 ------------WVRLCSERS--------LLSNIQMSEVQLPIVIDQFYCDKAKCSNQTS 488
                        VR+ + +         L SNIQ++EVQLPIVIDQFYCD +KC N TS
Sbjct: 390 TVRDVVMHNTMTGVRIKTWQGGIGSVKGILFSNIQLTEVQLPIVIDQFYCDHSKCMNHTS 449

BLAST of Csor.00g136000 vs. TAIR 10
Match: AT5G14650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 326.2 bits (835), Expect = 4.9e-89
Identity = 179/410 (43.66%), Postives = 221/410 (53.90%), Query Frame = 0

Query: 153 KGSDSAIFNVLHFGAKGNGESDDTKAFEDAWVAACKVEGSTVMVPA-------------- 212
           +G  S   NVL  GAKG+G SDDTKAFEDAW  ACKV  ST++VP+              
Sbjct: 44  EGGSSGTINVLDHGAKGDGTSDDTKAFEDAWQVACKVAASTLLVPSGSTFLVGPVSFLGK 103

Query: 213 ----DYVFFLDGTIMAPTDHRTWGKGLLEWIQFTKLIGVTIRGSGIIDGRGSVWWQDSSF 272
                 VF L+G I+APT    WG GLL+WI+F  L G+TI+G GIIDGRGSVWW D   
Sbjct: 104 ECKEKIVFQLEGKIIAPTSASAWGSGLLQWIEFKALQGITIKGKGIIDGRGSVWWNDM-- 163

Query: 273 DEPIDDEMKLIIPMNKTRDEAEPTPVGSNLEGKMPSIKPTALRFYGSFNVTVTGITIQNS 332
                                        +  KMP  KPTALRFYGS  VTV+GITIQNS
Sbjct: 164 -----------------------------MGTKMPRTKPTALRFYGSNGVTVSGITIQNS 223

Query: 333 PQCHLKFDNCVGVLVHDISVSSPGNSPNTDGIQLQNSKDVLIHSTSLSCG---------- 392
           PQ HLKFDNC+ + V D + SSPG+SPNTDGI LQNS+D +I+ ++L+CG          
Sbjct: 224 PQTHLKFDNCISIQVSDFTTSSPGDSPNTDGIHLQNSQDAVIYRSTLACGDDCISIQTGC 283

Query: 393 ---------------------------------------------WVRLCS--------E 452
                                                         VR+ S        +
Sbjct: 284 SNINIHDVDCGPGHGISIGGLGKDNTKACVSNITVRDVTMHETTNGVRIKSWQGGSGSVK 343

Query: 453 RSLLSNIQMSEVQLPIVIDQFYCDKAKCSNQTSGVALSGIDYEKIRGTYTVKPVHFACSD 482
           + + SNIQ+S V  PI+IDQ+YCD   C N+TS VA+S I+Y  I+GTYT +PV FACSD
Sbjct: 344 QVMFSNIQVSNVANPIIIDQYYCDGGGCHNETSAVAVSNINYINIKGTYTKEPVRFACSD 403

BLAST of Csor.00g136000 vs. TAIR 10
Match: AT3G26610.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 235.7 bits (600), Expect = 8.7e-62
Identity = 147/447 (32.89%), Postives = 213/447 (47.65%), Query Frame = 0

Query: 112 KSHHVSGSNPKAPPSHNASPSSPIPPKPKEEIIPTPPKKGYKGSDSAIFNVLHFGAKGNG 171
           + HHV    PK       +P+    P P            Y  ++  +FN+  +GAKG+G
Sbjct: 31  RKHHVKKIKPKHRRHSKNTPTGSPAPAP------------YPSTNEGVFNIFSYGAKGDG 90

Query: 172 ESDDTKAFEDAWVAACKVEGSTVMVPADYVFF-----------------LDGTIMAPTDH 231
            SDD+KA   AW AACKV G  V +PA   F                  ++G ++AP   
Sbjct: 91  VSDDSKALVGAWKAACKVVGGKVEIPAGTQFLVKAVTLQGPCKEETVVQIEGILVAPEKI 150

Query: 232 RTW-GKGLLEWIQFTKLIGVTIRGSGIIDGRGSVWWQDSSFDEPIDDEMKLIIPMNKTRD 291
            +W    L +W+ F  +  +TI+GSG ++GRG  WW   ++               +TR+
Sbjct: 151 GSWPNSSLFQWLNFKWVSHLTIQGSGTLNGRGYNWWNLDTY-------------QTQTRN 210

Query: 292 EAEPTPVGSNLEGKMPSIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCVGVLVHDIS 351
           +             +P +KPTALRFY S NVTV  I+I NSP CHLKFD+  GV V++I+
Sbjct: 211 K------------YIPPMKPTALRFYSSNNVTVRDISIVNSPLCHLKFDDSDGVKVNNIT 270

Query: 352 VSSPGNSPNTDGIQLQNSKDVLIHSTSLSCG----------------------------- 411
           +SSP NSPNTDGI LQN+++V I  ++++CG                             
Sbjct: 271 ISSPENSPNTDGIHLQNTRNVEIQHSNIACGDDCVSIQTGSSNVHIHHINCGPGHGISIG 330

Query: 412 --------------------------WVRLCS--------ERSLLSNIQMSEVQLPIVID 471
                                      VR+ +        +    SNIQ+ +V++PIVID
Sbjct: 331 GLGKDKSVACVSDIIVEDISIQNTLAGVRIKTWQGGLGVVKNLTFSNIQVKDVKVPIVID 390

Query: 472 QFYCDKAKCSNQTSGVALSGIDYEKIRGTYTVKPVHFACSDNQPCRDVTPTTIELKPL-- 473
           Q+YCDK+KC NQT  V++SG+ Y  I G++TV+PV  ACS+N PC DV    I L+P   
Sbjct: 391 QYYCDKSKCKNQTRAVSISGVKYNNIVGSFTVQPVRIACSNNVPCMDVDLMDIRLRPSGG 440

BLAST of Csor.00g136000 vs. TAIR 10
Match: AT1G48100.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 230.3 bits (586), Expect = 3.6e-60
Identity = 156/475 (32.84%), Postives = 212/475 (44.63%), Query Frame = 0

Query: 105 KGSHGGSKSHHVSGSNPKAPPSHNASPSSPIPPKPKEEIIPTPPKKGYKGSDSAIFNVLH 164
           KG HG   +H +    P  PP   A+P   +P  P     P+P           +F+V  
Sbjct: 33  KGRHG--VTHPL--PPPPPPPLETANPPDQVPSDP----YPSPDPAPGDSDSGCVFDVTS 92

Query: 165 FGAKGNGESDDTKAFEDAWVAACKVEGSTVMVP-----------------ADYVFFLDGT 224
           FGA G+G  DDT AF+DAW AAC VE   V+ P                    VF LDG 
Sbjct: 93  FGAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGGVFKITSTIFSGPCKPGLVFQLDGV 152

Query: 225 IMAPTDHRTWGK--GLLEWIQFTKLIGVTIRGSGIIDGRGSVWWQDSSFDEPIDDEMKLI 284
           +M P     W +     +W+ F +L G T  G G ++G G  WW                
Sbjct: 153 LMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWD--------------- 212

Query: 285 IPMNKTRDEAEPTPVGSNLEGKMPSIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCV 344
           +P    R      P GS+  G  P   PT +RF+ S N+ V G+ IQNSPQ H+KFD C 
Sbjct: 213 LPCKPHRG-----PDGSSSSG--PCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQ 272

Query: 345 GVLVHDISVSSPGNSPNTDGIQLQNSKDVLIHSTSLSCGWVRL-----CSE--------- 404
           GVL+++I +SSP  SPNTDGI L N++ V I+++ +S G   +     CS+         
Sbjct: 273 GVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCG 332

Query: 405 -------------------------------------------------RSLLSNIQMSE 464
                                                              L  NIQM  
Sbjct: 333 PSHGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMEN 392

Query: 465 VQLPIVIDQFYCDKAKCSNQTSGVALSGIDYEKIRGTYTVK--PVHFACSDNQPCRDVTP 496
           V   I++DQ+YC    C N+TS V +  ++Y  I+GTY V+  P+HFACSD   C ++T 
Sbjct: 393 VLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITM 452

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q949Z15.1e-5932.84Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV... [more]
Q94AJ53.5e-3927.05Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... [more]
P432122.1e-3627.84Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1[more]
Q7M1E71.2e-3428.23Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
Q9FY199.7e-3428.11Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAG6585162.10.0100.00Polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022951189.12.90e-30076.80polygalacturonase At1g48100-like isoform X2 [Cucurbita moschata][more]
XP_022951190.13.12e-27179.28polygalacturonase At1g48100-like isoform X3 [Cucurbita moschata][more]
XP_022951187.15.00e-27079.96polygalacturonase At1g48100-like isoform X1 [Cucurbita moschata][more]
XP_023537364.17.30e-27077.98polygalacturonase At1g48100-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023... [more]
Match NameE-valueIdentityDescription
A0A6J1GI031.40e-30076.80polygalacturonase At1g48100-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1GI001.51e-27179.28polygalacturonase At1g48100-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1GGZ42.42e-27079.96polygalacturonase At1g48100-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1HAQ27.45e-24771.03polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111462351 P... [more]
A0A6J1K7Y96.08e-24670.83polygalacturonase At1g48100-like OS=Cucurbita maxima OX=3661 GN=LOC111492497 PE=... [more]
Match NameE-valueIdentityDescription
AT1G10640.15.0e-13450.89Pectin lyase-like superfamily protein [more]
AT1G60590.16.1e-13250.89Pectin lyase-like superfamily protein [more]
AT5G14650.14.9e-8943.66Pectin lyase-like superfamily protein [more]
AT3G26610.18.7e-6232.89Pectin lyase-like superfamily protein [more]
AT1G48100.13.6e-6032.84Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 376..445
e-value: 4.8E-14
score: 52.2
coord: 291..362
e-value: 3.2E-13
score: 49.4
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 371..486
e-value: 5.9E-18
score: 67.0
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 148..364
e-value: 1.6E-50
score: 174.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 127..142
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 90..152
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 94..126
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 374..484
NoneNo IPR availablePANTHERPTHR31375:SF192PECTIN LYASE-LIKE SUPERFAMILY PROTEINcoord: 374..484
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 79..364
NoneNo IPR availablePANTHERPTHR31375:SF192PECTIN LYASE-LIKE SUPERFAMILY PROTEINcoord: 79..364
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 157..486

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g136000.m01Csor.00g136000.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006796 phosphate-containing compound metabolic process
molecular_function GO:0004650 polygalacturonase activity
molecular_function GO:0016740 transferase activity