Csor.00g134590 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g134590
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionpalmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like
LocationCsor_Chr14: 12709488 .. 12714764 (+)
RNA-Seq ExpressionCsor.00g134590
SyntenyCsor.00g134590
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGCATCAAAAGAACAAAATAGAGGCGTAAAGCCAATGTCATTCCCAAAGATAAAACCGCCTTTCGTTATGTTCTTTATACATTTTTTCTGTATTTTTGCACCCTTCCAAATTAGTTGGAGTGCAATTTCGATTTGTCTTATCTTATATATTATCACAGGTCTTTTTGGCATCACTATTTCGTATCATCGAAATCTTTCACATAAGAGTTTCAAAATTCCCAAATTGCTTGACTACTTGTTTGCTTACTGCGGAGTTCATGCACTTCAGGTTTGATAGTTGATACATTTTATTCTTTTGTTATTAAAAGTGACAAATTAGATACGTTCATGATGGATTTAAAAACAAATTTGTGTTAAAAGCACATGAAGATATATATTATCTTTCAAATATTTTTAATAATTTTTTTATATTAGGGTGATCCAATCTTTTGGGTGAGCACACATAGATATCATCATCAATACGTCCATACTGCAAAAGATCCACATAGTCCAATTAAAGGATTTTGGTTTAGTCATTTTACATGGTTTCTTCATAAGAGGGATCGAAGCACTAATGAAATAATTCCGAAACATCGAAAATGGGAAAATGCTACGGATTTGGAGAAGCAAACATTCTACAGGTTTCTTCACAAAACCTATTTACTTCATCCAATTGCTGTTGCGGTCATACTTTACAGCATTGGAGGCACTCCTTTCTTCATTTGGGGAACGGTAAAAGAAAAAATACCTCTATCTTTATTTGCTTAATTCCTTTTAGCTACAAATTTATAAGTCATACTAATTTTGTTAAACTTTGAACAACGAATTCAATTTCATTAAATAACACTACTGTGACTCATTCATAAGCGAAATACGGGATATACAATTACAAACTATGAGAAATGATTTTAAAATCTTTACTAAATAAATAAAGTAGATATTGTTTTTATTAAAAAATGTAACACACTATTTATTTTTTTTTTATTTTTTATTTTTGTTAAATAGTGTGTGAGAACCGTAATGATTCAACACGCAACATTCTTTGTGAATTCGGTATGTCATATGTGGGGAAAACGACCATGGAAAACAAACGATTTATCACGAAATAATTGGTATGTATCTTAGGTCAATCTTTATATCAATACTCAGTTGATCAAAGTTAATTCATTGCATGAGTTTTTGTTTCATGATAATATATATACACATTTTGTGTTTTCCTCCGTAGGTGGGTGAGTTTGCTTCTATTTGGAGAAGGTTGGCACAATAACCACCATGCTTTTGAGTATTCAGCTAGGATGGGGATTGAATGGTGGCAATATGATCCTGGTTGGTATGTTATTAGGTTTCTTCAAGCCATTAGAGTCGCCACTCATGTCAAATTACCATCCCAAACCCATATGCAAAAGTTGGCCAATGATTAACTAAAAAGGAGCCTCTCTTTGAGTGATGCTTTGTATGGAAATAATTATGCTCCAATAAATAATGTTTCAAAGTGTGGAATAAAATAAAATGGCAATATAAATTAGTTAATGCTATTCTATAATAAATTTTGATGGATGAATATATACTATGCACAATCACCGATGGACAACAAAACATAAAATCCTCACCGACCAACTTCCTCAAATCTCACCCCACTGTGTTACAATCTTCCTTGTGGTTTCATATCTAAGCCTCATCAACGATCGAATTGGAGTTTCGCCGTCTCTTCATGTTCGTTTTTGTTGTCTTTGGCTGATTGTGCCGCCACAATCTCTTCATTCTCCAATCCATTCATCATGAGGCTCCCTCAAATTTCGGCCTATGTTCCTTTCATAGAGAGGAAGATCTCACACTTTGAGAATGAAACTCTCATTCTCTCCTTCGCCAATCCTCTCATGAACGGTCGTTCTTTTGAAATTGTCATTCTCCCATTCCTTCGTGATTTTCTTTCAAGAGGTTACTACAAGTCTCTACTTTGTGTTCGAGTGATAGAAAGGAAGATATTGTGGAATGTGGAAGAAAATTTCTACATTAGAATACTTCAAGGAGTTCTGCCAATAAAGGCTTCTACAAAGGCAGAGTTCTTACTCATATTTATTGTTTTCTTTCAATTCTTAATAAAATCAAAACAGTTACCAATGCTTCCACTATATGGATAAAAAGTTTATTCCGTTCATTCCATTTAATAATATCTATGAATGTCTACGATATTATGCACACTCTTCCTCCGGTGGCTCTAACATTGTAGCATTCCCTAGTTAAAGAGAAGGCCTAAGGGGTACGTATACGCCCCCTAGAGAGTCCACATGTAGAGAACCACTACACATCCAATTTATGTCTTGACTAGAAAACAAAATTTAAGGCCATGATTACTTGTGTAATCATTGTCTGACTCTAATAGTAGGGTTACTTACTAAATATTTTTAAAAATACTTAAGACACGTGTTACTCTTATTTTTAAGATAAAGGTAAGGAACTTCTTATACAAATGACAGGGTGGCAATAGAGGCGGAAGGACCAAAGCTAGGATAGTTGCTTAAAATATTTCCAATGAACATTTTAGTTATTTAGGTGCGCAACCACACACCGACTCATGCGTGATTAATTCACGTGTGCACCTTTTTTTTTCATTTAAATTTTCAAGTTCTACAATGCGAGTTCATTCCATGAACGTGCTTGCATATGAATGTCAGAGGATCGAGATTTCTTTCGTTTTCATGAAATTGTGTTTTCATATATTTTTCTATAGAACTCTATTAAACTTGGACACTTCATCAAACCTAAAATGTGAAATTCTTAATGGATGCTATGAATATAAAGGGGCCATAGGTTGGGTGGCGTAGGGTAAAATATTCAAACCCAACTCCTTAGATTGCTCCACCAACATGTTATCTCTCCATTTAATAACTAATTCCCTCACATACAAGCTAGAACTCAGAATGGCAATTCCAGCTATAGAATTGAAATCCACGATATTAATGAATTTTGAGTTAACTCAACAAAGATCAAACCTGACATATGCAGGAGATCGGTAATTTTGATTCGTTTCTAAAAAGAACAATTCCTTCTTTGTTTTTTGTTTCCTTCTGTCTCCCCAAAATTTCAAGGATGGTTGGGAAGGAGTCCCGCATTAGCTAATTCTTGAAATGATCATGGATTTATAAATAAGAAATACATCTCTATTACTCTTAGGTCTTTTTGAAAAGCTCAAAGCAAAGCTACAAGAACTTAGACTAATTTTGAGAACTTCTAATGGAACGAAGAACCAATTTATTAACTTTACTTTACCAACAGGTTAGCTCCACTTTATAACCTAACTCATCAATCCAATCCAAACCCCCTCCAGCTATGCTAAAATTAATAGTGGAATATAATATAGTTGTTCTTTAAAATAGTGAAAATAATTAGTTTAAATGTATCATAAATGATTATTATTGTTGTTATTATATATAGCTATGTATTTGTCCATGATATTTGACGATTTTGAAGCAGGTATATTGCACTTAAATAAGCTATACATTTAGTTGGAAGAGCCTTAAATTTTTACATCGAGATTTAAAAGTTTCACTATAAATACATATGCAAGAGGAGGTGGTGAAGCCTCCTCAACTAGAGCAACCCCACTTTAATGGATGCATCAAGAGAACCAAAGAGAATTGTGAAGCCTCCAATGTCATTTGGGAAGAGAAAATGGCCCAAGATAAAGAAAAAAATTGTTTTCATTCTTTTCTTTATGCATTTTTTCTGTATTTTTGCACCCTTTCAAGTCAATTTGGGTGCAATTTCGATTTGCTTTGCCTTATATAATATTACAGGTCTTTTTGGACTCACTATTTCATATCATCGGAATCTTACACATAAAAGTTTCGTACTTCCGAAATGGCTTGAATACTTGTTTGCATATTGTGGTGTTCATAGTTTTCAGGTTTGACAAACTTTATTCTCAAATTAAATGCATTCAAGATTGATTTTAAAAGCACGTTGATTAGTTCTAAATATTTGTTGATAGGGTGATCCAATCTATTGGGTCAGCACACATAGATATCATCATCAATATGTTGATACTCCAAAAGATCCACATAGTCCAATTAAAGGATTTTGGTATAGTCATCTTACTTGGTATCTTCATAAAAGGGATCGAAGCGTTAATGAAATGATTCCGAGACGTCGAAAATGGGAAAATGCTACAGATTTGGAGAAGCAAACATTCTATAGGTTTCTTCACAAAACATATATATATCATCCCATTGCTCTTGCGGTCATACTTTATAGCATTGGAGGCGCTCCTTTCCTCATTTGGGGAATGGTATGAAAAAAACTACCTCTTTCTTCATTTGTTTATTTCCTTTTAGTTACAATTTTATATGTTATGATAATTTGATAAATAACTTTACAAAAATACTTTCAATTTGATCAAATAACACGTCCGGTTATTTTACTTTTTAGCTCAATTTTTAGAAATTAAATTTGTTTATAACCTAAATATTACAAAGCTCCGAATTCAACTGAACGAATATATATGAAATATGATTTTAAATATTTTTAGAAATAAATAAAGTAGATATTGTGATTATTAGAAATATGGGGTATAAAATGTGACATATTATTCTTTTTGTTCGACAGTGTGTGAGAATTGTAGTGGGTTTACATGCAACATTCTTGGTGAATTCAGTATCTCATATGTGGGGAAAGAAACCATGGAAAACGAACGATTTATCAAGAAACAATTGGTATGTATCGTTGTTGAGTAAAGGATTACCGAATCAATTTAGAGTTTGTATTTTTAATAAATTTTAAAAACAGTTCTCCTCGAAATGAAAAGAAAAGTGACTTTTAATTAAAAATTTTAAATTTAATCTCTTTCCATAAATCTTAAAAATAGTTTATATATTATTTTTGCCAAACAAAAGATGATCTCATTGGTCTTTCCTTCTATAAAATTTCAATACATTTTTACATAGTTCTCTTCCTGAAATTTATTACTATTATTCCCAATTTAGATAAAACTTAAAGTCTGAACATCTAAATTTAAATTTTAGAATCGCTAAAATTGAATTATATTTTTCTATTTATCATAATCTTCTTCTTTTTTTTTTTCTTTTTTTTTTTAAATAGGTGGATAAGTTTTCTTGTATTTGGAGAAGGTTGGCACAATAACCACCATGCTTTCGAGTACTCAGCTAGGTTGGGCATTGAATGGTGGCAATATGATCCTGGTTGGTATGTCATTAGGTTTCTTGAAGCCATTGGAGTGGCCACTCATGTCAAATTGCCATCTCAAACCCATATGCAAAAATTAGCCAAAGATTAG

mRNA sequence

ATGGATGCATCAAAAGAACAAAATAGAGGCGTAAAGCCAATGTCATTCCCAAAGATAAAACCGCCTTTCGTTATGTTCTTTATACATTTTTTCTGTCTTTTTGGCATCACTATTTCGTATCATCGAAATCTTTCACATAAGAGTTTCAAAATTCCCAAATTGCTTGACTACTTGTTTGCTTACTGCGGAGTTCATGCACTTCAGGGTGATCCAATCTTTTGGGTGAGCACACATAGATATCATCATCAATACGTCCATACTGCAAAAGATCCACATAGTCCAATTAAAGGATTTTGGTTTAGTCATTTTACATGGTTTCTTCATAAGAGGGATCGAAGCACTAATGAAATAATTCCGAAACATCGAAAATGGGAAAATGCTACGGATTTGGAGAAGCAAACATTCTACAGGTTTCTTCACAAAACCTATTTACTTCATCCAATTGCTGTTGCGGTCATACTTTACAGCATTGGAGGCACTCCTTTCTTCATTTGGGGAACGTGTGTGAGAACCGTAATGATTCAACACGCAACATTCTTTGTGAATTCGGTATGTCATATGTGGGGAAAACGACCATGGAAAACAAACGATTTATCACGAAATAATTGGTGGGTGAGTTTGCTTCTATTTGGAGAAGGTTGGCACAATAACCACCATGCTTTTGAGTATTCAGCTAGGATGGGGATTGAATGGTGGCAATATGATCCTGGTTGGGATCGAAGCGTTAATGAAATGATTCCGAGACGTCGAAAATGGGAAAATGCTACAGATTTGGAGAAGCAAACATTCTATAGGTTTCTTCACAAAACATATATATATCATCCCATTGCTCTTGCGGTCATACTTTATAGCATTGGAGGCGCTCCTTTCCTCATTTGGGGAATGTGTGTGAGAATTGTAGTGGGTTTACATGCAACATTCTTGGTGAATTCAGTATCTCATATGTGGGGAAAGAAACCATGGAAAACGAACGATTTATCAAGAAACAATTGGTGGATAAGTTTTCTTGTATTTGGAGAAGGTTGGCACAATAACCACCATGCTTTCGAGTACTCAGCTAGGTTGGGCATTGAATGGTGGCAATATGATCCTGGTTGGTATGTCATTAGGTTTCTTGAAGCCATTGGAGTGGCCACTCATGTCAAATTGCCATCTCAAACCCATATGCAAAAATTAGCCAAAGATTAG

Coding sequence (CDS)

ATGGATGCATCAAAAGAACAAAATAGAGGCGTAAAGCCAATGTCATTCCCAAAGATAAAACCGCCTTTCGTTATGTTCTTTATACATTTTTTCTGTCTTTTTGGCATCACTATTTCGTATCATCGAAATCTTTCACATAAGAGTTTCAAAATTCCCAAATTGCTTGACTACTTGTTTGCTTACTGCGGAGTTCATGCACTTCAGGGTGATCCAATCTTTTGGGTGAGCACACATAGATATCATCATCAATACGTCCATACTGCAAAAGATCCACATAGTCCAATTAAAGGATTTTGGTTTAGTCATTTTACATGGTTTCTTCATAAGAGGGATCGAAGCACTAATGAAATAATTCCGAAACATCGAAAATGGGAAAATGCTACGGATTTGGAGAAGCAAACATTCTACAGGTTTCTTCACAAAACCTATTTACTTCATCCAATTGCTGTTGCGGTCATACTTTACAGCATTGGAGGCACTCCTTTCTTCATTTGGGGAACGTGTGTGAGAACCGTAATGATTCAACACGCAACATTCTTTGTGAATTCGGTATGTCATATGTGGGGAAAACGACCATGGAAAACAAACGATTTATCACGAAATAATTGGTGGGTGAGTTTGCTTCTATTTGGAGAAGGTTGGCACAATAACCACCATGCTTTTGAGTATTCAGCTAGGATGGGGATTGAATGGTGGCAATATGATCCTGGTTGGGATCGAAGCGTTAATGAAATGATTCCGAGACGTCGAAAATGGGAAAATGCTACAGATTTGGAGAAGCAAACATTCTATAGGTTTCTTCACAAAACATATATATATCATCCCATTGCTCTTGCGGTCATACTTTATAGCATTGGAGGCGCTCCTTTCCTCATTTGGGGAATGTGTGTGAGAATTGTAGTGGGTTTACATGCAACATTCTTGGTGAATTCAGTATCTCATATGTGGGGAAAGAAACCATGGAAAACGAACGATTTATCAAGAAACAATTGGTGGATAAGTTTTCTTGTATTTGGAGAAGGTTGGCACAATAACCACCATGCTTTCGAGTACTCAGCTAGGTTGGGCATTGAATGGTGGCAATATGATCCTGGTTGGTATGTCATTAGGTTTCTTGAAGCCATTGGAGTGGCCACTCATGTCAAATTGCCATCTCAAACCCATATGCAAAAATTAGCCAAAGATTAG

Protein sequence

MDASKEQNRGVKPMSFPKIKPPFVMFFIHFFCLFGITISYHRNLSHKSFKIPKLLDYLFAYCGVHALQGDPIFWVSTHRYHHQYVHTAKDPHSPIKGFWFSHFTWFLHKRDRSTNEIIPKHRKWENATDLEKQTFYRFLHKTYLLHPIAVAVILYSIGGTPFFIWGTCVRTVMIQHATFFVNSVCHMWGKRPWKTNDLSRNNWWVSLLLFGEGWHNNHHAFEYSARMGIEWWQYDPGWDRSVNEMIPRRRKWENATDLEKQTFYRFLHKTYIYHPIALAVILYSIGGAPFLIWGMCVRIVVGLHATFLVNSVSHMWGKKPWKTNDLSRNNWWISFLVFGEGWHNNHHAFEYSARLGIEWWQYDPGWYVIRFLEAIGVATHVKLPSQTHMQKLAKD
Homology
BLAST of Csor.00g134590 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 300.1 bits (767), Expect = 3.8e-80
Identity = 129/206 (62.62%), Postives = 155/206 (75.24%), Query Frame = 0

Query: 33  LFGITISYHRNLSHKSFKIPKLLDYLFAYCGVHALQGDPIFWVSTHRYHHQYVHTAKDPH 92
           L GIT+S+HRNLSHK+FK+PK L+YLFAYCG  ALQG+PI WVSTHRYHHQ+  + +DPH
Sbjct: 140 LLGITLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPH 199

Query: 93  SPIKGFWFSHFTWFLHKRDRSTNEIIPKHRKWENATDLEKQTFYRFLHKTYLLHPIAVAV 152
           SP+ GFWFSH  W        TN I  +  +  N  DLEKQ FYRFL  TY+LHP+A+AV
Sbjct: 200 SPLDGFWFSHMNWMF-----DTNTITQRCGEPNNVGDLEKQPFYRFLRTTYILHPLALAV 259

Query: 153 ILYSIGGTPFFIWGTCVRTVMIQHATFFVNSVCHMWGKRPWKTNDLSRNNWWVSLLLFGE 212
            LY++GG PF +WG  VR V + H T+ VNS CH+WGK+ W T DLS+NNWWV+ L FGE
Sbjct: 260 ALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAALAFGE 319

Query: 213 GWHNNHHAFEYSARMGIEWWQYDPGW 239
           GWHNNHHAFE+SAR G+EWWQ D  W
Sbjct: 320 GWHNNHHAFEFSARHGLEWWQLDMTW 340

BLAST of Csor.00g134590 vs. ExPASy Swiss-Prot
Match: P0DOW2 (Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 3.3e-76
Identity = 125/217 (57.60%), Postives = 158/217 (72.81%), Query Frame = 0

Query: 23  FVMFFIHFFC-LFGITISYHRNLSHKSFKIPKLLDYLFAYCGVHALQGDPIFWVSTHRYH 82
           +V F ++  C +FG T+S+HRNL+H+SFK+PK L+Y FAY G+HALQGDP++WVSTHRYH
Sbjct: 71  WVCFALYAICGVFGTTLSFHRNLTHRSFKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYH 130

Query: 83  HQYVHTAKDPHSPIKGFWFSHFTWFLHKRDRSTNEIIPKHRKWENATDLEKQTFYRFLHK 142
           H+Y  T  DPHSPI+GFWF H  W        +  II K  ++ENA DL KQ++YRFL +
Sbjct: 131 HKYTDTYLDPHSPIEGFWFCHIFWLF-----DSKYIIEKCGRYENAGDLMKQSYYRFLER 190

Query: 143 TYLLHPIAVAVILYSIGGTPFFIWGTCVRTVMIQHATFFVNSVCHMWGKRPWKTNDLSRN 202
           T++ H    A +LY  GG PF +WG  VRT++  H ++ VNSVCH WG RPW T DLS N
Sbjct: 191 TFVYHVYLQAALLYLFGGFPFIVWGMAVRTILGFHLSWLVNSVCHRWGNRPWNTGDLSTN 250

Query: 203 NWWVSLLLFGEGWHNNHHAFEYSARMGIEWWQYDPGW 239
           NW++++L  GEGWHNNHHAFEYSAR GIEWWQ D  W
Sbjct: 251 NWFIAMLTSGEGWHNNHHAFEYSARHGIEWWQIDTTW 282

BLAST of Csor.00g134590 vs. ExPASy Swiss-Prot
Match: P0DOW3 (Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 9.1e-74
Identity = 120/206 (58.25%), Postives = 152/206 (73.79%), Query Frame = 0

Query: 33  LFGITISYHRNLSHKSFKIPKLLDYLFAYCGVHALQGDPIFWVSTHRYHHQYVHTAKDPH 92
           +FG T+SYHRNL+H+SFK+PK L+Y FAY G+HALQGDP++WVSTHRYHH++  T  DPH
Sbjct: 79  VFGTTLSYHRNLTHRSFKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKFTDTYLDPH 138

Query: 93  SPIKGFWFSHFTWFLHKRDRSTNEIIPKHRKWENATDLEKQTFYRFLHKTYLLHPIAVAV 152
           SPI+GFWFSH  W        +  I+ +  ++ENA DL KQ++YRFL +T++ H    A 
Sbjct: 139 SPIEGFWFSHIFWLF-----DSKYILEECGRYENAGDLLKQSYYRFLERTFVFHVYLQAA 198

Query: 153 ILYSIGGTPFFIWGTCVRTVMIQHATFFVNSVCHMWGKRPWKTNDLSRNNWWVSLLLFGE 212
           +LY  GG PF +WG  VRTV   HA++ VNSVCH +G + W T DLS NNW++++L  GE
Sbjct: 199 LLYMFGGFPFIVWGMAVRTVWGFHASWLVNSVCHRYGHQAWDTGDLSTNNWFIAMLTSGE 258

Query: 213 GWHNNHHAFEYSARMGIEWWQYDPGW 239
           GWHNNHHAFEYSAR GIEWWQ D  W
Sbjct: 259 GWHNNHHAFEYSARHGIEWWQIDTTW 279

BLAST of Csor.00g134590 vs. ExPASy Swiss-Prot
Match: Q9LVZ3 (Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3.2 PE=2 SV=3)

HSP 1 Score: 266.9 bits (681), Expect = 3.6e-70
Identity = 119/214 (55.61%), Postives = 146/214 (68.22%), Query Frame = 0

Query: 25  MFFIHFFCLFGITISYHRNLSHKSFKIPKLLDYLFAYCGVHALQGDPIFWVSTHRYHHQY 84
           + FI+  C   IT+SYHRNLSH+SF +PK L+YLFAY GV A QGDPI WVS HRYHH++
Sbjct: 127 LVFINGIC---ITLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKH 186

Query: 85  VHTAKDPHSPIKGFWFSHFTWFLHKRDRSTNEIIPKHRKWENATDLEKQTFYRFLHKTYL 144
             T +DPHSP +GFWFSH  W        T+ I+      EN  DL +Q FYRFL +T L
Sbjct: 187 CETQRDPHSPTQGFWFSHMAWIF-----DTSSILENCGGEENVDDLVRQPFYRFLQRTVL 246

Query: 145 LHPIAVAVILYSIGGTPFFIWGTCVRTVMIQHATFFVNSVCHMWGKRPWKTNDLSRNNWW 204
           LH +A + + Y  GG P  +WG  +   +  H TF VNSVCH+WG R W T+D S+NNWW
Sbjct: 247 LHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFSKNNWW 306

Query: 205 VSLLLFGEGWHNNHHAFEYSARMGIEWWQYDPGW 239
           V++L  GEGWHNNHHAFE+SAR G+EWWQ D  W
Sbjct: 307 VAILTLGEGWHNNHHAFEFSARHGLEWWQLDITW 332

BLAST of Csor.00g134590 vs. ExPASy Swiss-Prot
Match: O65797 (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 6.1e-70
Identity = 118/238 (49.58%), Postives = 147/238 (61.76%), Query Frame = 0

Query: 23  FVMFFIHFFCL----------------------FGITISYHRNLSHKSFKIPKLLDYLFA 82
           F   F+HF CL                       GIT+SYHRNL+H+SFK+PK L+Y FA
Sbjct: 43  FASLFVHFLCLLAPFNFTWPALRVALIVYTVGGLGITVSYHRNLAHRSFKVPKWLEYFFA 102

Query: 83  YCGVHALQGDPIFWVSTHRYHHQYVHTAKDPHSPIKGFWFSHFTWFLHKRDRSTNEIIPK 142
           YCG+ A+QGDPI WVSTHRYHHQ+  + +DPHSP +GFWFSH  W        T  ++ K
Sbjct: 103 YCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGFWFSHLLWLF-----DTGYLVEK 162

Query: 143 HRKWENATDLEKQTFYRFLHKTYLLHPIAVAVILYSIGGTPFFIWGTCVRTVMIQHATFF 202
             +  N  DL++Q +Y+FL +T L H +    +LY  GG  F  WG  +   M  H T  
Sbjct: 163 CGRRTNVEDLKRQWYYKFLQRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCL 222

Query: 203 VNSVCHMWGKRPWKTNDLSRNNWWVSLLLFGEGWHNNHHAFEYSARMGIEWWQYDPGW 239
           +NS+CH+WG R WKTND SRN WW+S+  FGE WHNNHHAFE SAR G+EWWQ D  W
Sbjct: 223 INSLCHVWGSRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISW 275

BLAST of Csor.00g134590 vs. NCBI nr
Match: KAG6581936.1 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 867 bits (2240), Expect = 0.0
Identity = 395/395 (100.00%), Postives = 395/395 (100.00%), Query Frame = 0

Query: 1   MDASKEQNRGVKPMSFPKIKPPFVMFFIHFFCLFGITISYHRNLSHKSFKIPKLLDYLFA 60
           MDASKEQNRGVKPMSFPKIKPPFVMFFIHFFCLFGITISYHRNLSHKSFKIPKLLDYLFA
Sbjct: 1   MDASKEQNRGVKPMSFPKIKPPFVMFFIHFFCLFGITISYHRNLSHKSFKIPKLLDYLFA 60

Query: 61  YCGVHALQGDPIFWVSTHRYHHQYVHTAKDPHSPIKGFWFSHFTWFLHKRDRSTNEIIPK 120
           YCGVHALQGDPIFWVSTHRYHHQYVHTAKDPHSPIKGFWFSHFTWFLHKRDRSTNEIIPK
Sbjct: 61  YCGVHALQGDPIFWVSTHRYHHQYVHTAKDPHSPIKGFWFSHFTWFLHKRDRSTNEIIPK 120

Query: 121 HRKWENATDLEKQTFYRFLHKTYLLHPIAVAVILYSIGGTPFFIWGTCVRTVMIQHATFF 180
           HRKWENATDLEKQTFYRFLHKTYLLHPIAVAVILYSIGGTPFFIWGTCVRTVMIQHATFF
Sbjct: 121 HRKWENATDLEKQTFYRFLHKTYLLHPIAVAVILYSIGGTPFFIWGTCVRTVMIQHATFF 180

Query: 181 VNSVCHMWGKRPWKTNDLSRNNWWVSLLLFGEGWHNNHHAFEYSARMGIEWWQYDPGWDR 240
           VNSVCHMWGKRPWKTNDLSRNNWWVSLLLFGEGWHNNHHAFEYSARMGIEWWQYDPGWDR
Sbjct: 181 VNSVCHMWGKRPWKTNDLSRNNWWVSLLLFGEGWHNNHHAFEYSARMGIEWWQYDPGWDR 240

Query: 241 SVNEMIPRRRKWENATDLEKQTFYRFLHKTYIYHPIALAVILYSIGGAPFLIWGMCVRIV 300
           SVNEMIPRRRKWENATDLEKQTFYRFLHKTYIYHPIALAVILYSIGGAPFLIWGMCVRIV
Sbjct: 241 SVNEMIPRRRKWENATDLEKQTFYRFLHKTYIYHPIALAVILYSIGGAPFLIWGMCVRIV 300

Query: 301 VGLHATFLVNSVSHMWGKKPWKTNDLSRNNWWISFLVFGEGWHNNHHAFEYSARLGIEWW 360
           VGLHATFLVNSVSHMWGKKPWKTNDLSRNNWWISFLVFGEGWHNNHHAFEYSARLGIEWW
Sbjct: 301 VGLHATFLVNSVSHMWGKKPWKTNDLSRNNWWISFLVFGEGWHNNHHAFEYSARLGIEWW 360

Query: 361 QYDPGWYVIRFLEAIGVATHVKLPSQTHMQKLAKD 395
           QYDPGWYVIRFLEAIGVATHVKLPSQTHMQKLAKD
Sbjct: 361 QYDPGWYVIRFLEAIGVATHVKLPSQTHMQKLAKD 395

BLAST of Csor.00g134590 vs. NCBI nr
Match: KAG6581935.1 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 596 bits (1536), Expect = 2.72e-210
Identity = 302/501 (60.28%), Postives = 326/501 (65.07%), Query Frame = 0

Query: 1   MDASKEQNRGVKP-MSFPKIK-------PPFVMFFIHFFC-------------------- 60
           MDASKE    VKP MSF K K         F  FF+HF C                    
Sbjct: 1   MDASKEPKSIVKPPMSFGKKKWTKIDKKVAFTFFFLHFLCIFAPFQINWSAISISFTLYI 60

Query: 61  ---LFGITISYHRNLSHKSFKIPKLLDYLFAYCGVHALQGDPIFWVSTHRYHHQYVHTAK 120
              LFGITISYHRNLSHKSFKIPK L+YLFAYCGVHA QGDPI WVSTHRYHHQ+V T K
Sbjct: 61  IIGLFGITISYHRNLSHKSFKIPKWLEYLFAYCGVHAFQGDPINWVSTHRYHHQFVDTEK 120

Query: 121 DPHSPIKGFWFSHFTWFLHKRDRSTNEIIPKHRKWENATDLEKQTFYRFLHKTYLLHPIA 180
           DPHSPIKGFW SH  W L+K D S +E++ +HRKW+NA DLE QTFYRF+HKTY LHPIA
Sbjct: 121 DPHSPIKGFWSSHIIWLLYKGDGSLSEMLGRHRKWDNACDLENQTFYRFIHKTYFLHPIA 180

Query: 181 VAVILYSIGGTPFFIWGTCVRTVMIQHATFFVNSVCHMWGKRPWKTNDLSRNNWWVSLLL 240
           +A+ILY                            VCH+WGK+ WKTNDLSRNNWW+SLL+
Sbjct: 181 LALILY----------------------------VCHIWGKQQWKTNDLSRNNWWISLLV 240

Query: 241 FGEGWHNNHHAFEYSARMGIEWWQYDPG-------------------------------- 300
           FGEGWHNNHHAFEYSAR+G+EWWQYDPG                                
Sbjct: 241 FGEGWHNNHHAFEYSARLGMEWWQYDPGLFGITISYHRNLTHKTFKLPKWLEYLLAYCGL 300

Query: 301 ----------------------------------------W-----DRSVNEMIPRRRKW 360
                                                   W     D+SV+EMIPR RKW
Sbjct: 301 HALQGDPIYWVSIHRYHHQYVDTPTDPHSRIKGFWFSHLTWYLQKRDQSVSEMIPRDRKW 360

Query: 361 ENATDLEKQTFYRFLHKTYIYHPIALAVILYSIGGAPFLIWGMCVRIVVGLHATFLVNSV 393
           EN TDL+KQTFYRFLHKTY+ HPIALA ILYSIGGAPF IWGMCVRIV+ LHATFLVNSV
Sbjct: 361 ENVTDLKKQTFYRFLHKTYLLHPIALAAILYSIGGAPFFIWGMCVRIVIVLHATFLVNSV 420

BLAST of Csor.00g134590 vs. NCBI nr
Match: XP_022955965.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 511 bits (1315), Expect = 1.39e-179
Identity = 261/418 (62.44%), Postives = 263/418 (62.92%), Query Frame = 0

Query: 1   MDASKEQNRGVKPMSFPKIKPPFVMFFIHFFC-----------------------LFGIT 60
           MDASKEQNRGVKPMSFPKIKPPFVMFFIHFFC                       LFGIT
Sbjct: 1   MDASKEQNRGVKPMSFPKIKPPFVMFFIHFFCIFAPFQISWSAISICLILYIITGLFGIT 60

Query: 61  ISYHRNLSHKSFKIPKLLDYLFAYCGVHALQGDPIFWVSTHRYHHQYVHTAKDPHSPIKG 120
           ISYHRNLSHKSFKIPKLL+YLFAYCGVHALQGDPIFWVSTHRYHHQYV T KDPHSPIKG
Sbjct: 61  ISYHRNLSHKSFKIPKLLEYLFAYCGVHALQGDPIFWVSTHRYHHQYVDTPKDPHSPIKG 120

Query: 121 FWFSHFTWFLHKRDRSTNEIIPKHRKWENATDLEKQTFYRFLHKTYLLHPIAVAVILYSI 180
           FWFSHFTWFLHKRDRS NEIIPKHRKWENATDLEKQTFYRFLHKTYLLHPIAVAVILYSI
Sbjct: 121 FWFSHFTWFLHKRDRSANEIIPKHRKWENATDLEKQTFYRFLHKTYLLHPIAVAVILYSI 180

Query: 181 GGTPFFIWGTCVRTVMIQHATFFVNSVCHMWGKRPWKTNDLSRNNWWVSLLLFGEGWHNN 240
           GGTPFFIWGTCVRTVMIQHATFFVNSVCHMWGKRPWKTNDLSRNNWWVSLLLFGEGWHNN
Sbjct: 181 GGTPFFIWGTCVRTVMIQHATFFVNSVCHMWGKRPWKTNDLSRNNWWVSLLLFGEGWHNN 240

Query: 241 HHAFEYSARMGIEWWQYDPGWDRSVNEMIPRRRKWENATDLEKQTFYRFLHKTYIYHPIA 300
           HHAFEYSARMGIEWWQY                                           
Sbjct: 241 HHAFEYSARMGIEWWQY------------------------------------------- 290

Query: 301 LAVILYSIGGAPFLIWGMCVRIVVGLHATFLVNSVSHMWGKKPWKTNDLSRNNWWISFLV 360
                                                                       
Sbjct: 301 ------------------------------------------------------------ 290

Query: 361 FGEGWHNNHHAFEYSARLGIEWWQYDPGWYVIRFLEAIGVATHVKLPSQTHMQKLAKD 395
                                    DPGWYVIRFL+AIGVATHVKLPSQTHMQKLA D
Sbjct: 361 -------------------------DPGWYVIRFLQAIGVATHVKLPSQTHMQKLAND 290

BLAST of Csor.00g134590 vs. NCBI nr
Match: XP_023529140.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 486 bits (1251), Expect = 4.74e-170
Identity = 249/405 (61.48%), Postives = 251/405 (61.98%), Query Frame = 0

Query: 14  MSFPKIKPPFVMFFIHFFC-----------------------LFGITISYHRNLSHKSFK 73
           MSFPKIKPPFVMFFIHFFC                       LFGITISYHRNLSHKSFK
Sbjct: 1   MSFPKIKPPFVMFFIHFFCIFAPFQISWSAISICLILYIITGLFGITISYHRNLSHKSFK 60

Query: 74  IPKLLDYLFAYCGVHALQGDPIFWVSTHRYHHQYVHTAKDPHSPIKGFWFSHFTWFLHKR 133
           IPKLL+YLFAYCGVHALQGDPIFWVSTHRYHHQYV TAKDPHSPIKGFWFSHFTWFLHKR
Sbjct: 61  IPKLLEYLFAYCGVHALQGDPIFWVSTHRYHHQYVDTAKDPHSPIKGFWFSHFTWFLHKR 120

Query: 134 DRSTNEIIPKHRKWENATDLEKQTFYRFLHKTYLLHPIAVAVILYSIGGTPFFIWGTCVR 193
           DRS NEIIPKHRKWENATDLEKQTFYRFLHKTYLLHPIAVAVILYSIGGTPFFIWGTCVR
Sbjct: 121 DRSANEIIPKHRKWENATDLEKQTFYRFLHKTYLLHPIAVAVILYSIGGTPFFIWGTCVR 180

Query: 194 TVMIQHATFFVNSVCHMWGKRPWKTNDLSRNNWWVSLLLFGEGWHNNHHAFEYSARMGIE 253
           TVMIQHATFFVNSVCHMWGKRPWKTNDLSRNNWWVSLLLFGEGWHNNHHAFEYSARMGIE
Sbjct: 181 TVMIQHATFFVNSVCHMWGKRPWKTNDLSRNNWWVSLLLFGEGWHNNHHAFEYSARMGIE 240

Query: 254 WWQYDPGWDRSVNEMIPRRRKWENATDLEKQTFYRFLHKTYIYHPIALAVILYSIGGAPF 313
           WWQY                                                        
Sbjct: 241 WWQY-------------------------------------------------------- 277

Query: 314 LIWGMCVRIVVGLHATFLVNSVSHMWGKKPWKTNDLSRNNWWISFLVFGEGWHNNHHAFE 373
                                                                       
Sbjct: 301 ------------------------------------------------------------ 277

Query: 374 YSARLGIEWWQYDPGWYVIRFLEAIGVATHVKLPSQTHMQKLAKD 395
                       DPGWYVIRFL+AIGVATHVKLPSQTHMQKLA D
Sbjct: 361 ------------DPGWYVIRFLQAIGVATHVKLPSQTHMQKLAND 277

BLAST of Csor.00g134590 vs. NCBI nr
Match: XP_022980469.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 467 bits (1201), Expect = 3.06e-162
Identity = 241/418 (57.66%), Postives = 252/418 (60.29%), Query Frame = 0

Query: 1   MDASKEQNRGVKPMSFPKIKPPFVMFFIHFFC-----------------------LFGIT 60
           MDAS +QNR VKPMSFPKI   FV+FFIHFFC                       LFGIT
Sbjct: 1   MDASNKQNRVVKPMSFPKIIRLFVLFFIHFFCIFAPFQINWSAISVCFILYIITGLFGIT 60

Query: 61  ISYHRNLSHKSFKIPKLLDYLFAYCGVHALQGDPIFWVSTHRYHHQYVHTAKDPHSPIKG 120
           ISYHRNLSHKSFKI K L+YLFAYCGVHA QGDPIFWVSTHRYHHQYV T KDPHSPIKG
Sbjct: 61  ISYHRNLSHKSFKISKWLEYLFAYCGVHAFQGDPIFWVSTHRYHHQYVDTPKDPHSPIKG 120

Query: 121 FWFSHFTWFLHKRDRSTNEIIPKHRKWENATDLEKQTFYRFLHKTYLLHPIAVAVILYSI 180
           FWFSHFTWFLHK DR+ +E+IP+HRKWENATDLEKQTFYRFLHKTY+LHPIAVAVILYSI
Sbjct: 121 FWFSHFTWFLHKMDRTVDEMIPRHRKWENATDLEKQTFYRFLHKTYMLHPIAVAVILYSI 180

Query: 181 GGTPFFIWGTCVRTVMIQHATFFVNSVCHMWGKRPWKTNDLSRNNWWVSLLLFGEGWHNN 240
           GGTPFFIWG CVRTVMIQHATFFVNSVCHMWGK+PWKTNDLSRNNW VSLL+FGEGWHNN
Sbjct: 181 GGTPFFIWGMCVRTVMIQHATFFVNSVCHMWGKQPWKTNDLSRNNWVVSLLIFGEGWHNN 240

Query: 241 HHAFEYSARMGIEWWQYDPGWDRSVNEMIPRRRKWENATDLEKQTFYRFLHKTYIYHPIA 300
           HHAFEYSARMGIEWWQY                                           
Sbjct: 241 HHAFEYSARMGIEWWQY------------------------------------------- 290

Query: 301 LAVILYSIGGAPFLIWGMCVRIVVGLHATFLVNSVSHMWGKKPWKTNDLSRNNWWISFLV 360
                                                                       
Sbjct: 301 ------------------------------------------------------------ 290

Query: 361 FGEGWHNNHHAFEYSARLGIEWWQYDPGWYVIRFLEAIGVATHVKLPSQTHMQKLAKD 395
                                    DPGWYVIRFL+AIGVATHVKLPSQTHMQKLA D
Sbjct: 361 -------------------------DPGWYVIRFLQAIGVATHVKLPSQTHMQKLAND 290

BLAST of Csor.00g134590 vs. ExPASy TrEMBL
Match: A0A6J1GWJ3 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111457797 PE=3 SV=1)

HSP 1 Score: 511 bits (1315), Expect = 6.74e-180
Identity = 261/418 (62.44%), Postives = 263/418 (62.92%), Query Frame = 0

Query: 1   MDASKEQNRGVKPMSFPKIKPPFVMFFIHFFC-----------------------LFGIT 60
           MDASKEQNRGVKPMSFPKIKPPFVMFFIHFFC                       LFGIT
Sbjct: 1   MDASKEQNRGVKPMSFPKIKPPFVMFFIHFFCIFAPFQISWSAISICLILYIITGLFGIT 60

Query: 61  ISYHRNLSHKSFKIPKLLDYLFAYCGVHALQGDPIFWVSTHRYHHQYVHTAKDPHSPIKG 120
           ISYHRNLSHKSFKIPKLL+YLFAYCGVHALQGDPIFWVSTHRYHHQYV T KDPHSPIKG
Sbjct: 61  ISYHRNLSHKSFKIPKLLEYLFAYCGVHALQGDPIFWVSTHRYHHQYVDTPKDPHSPIKG 120

Query: 121 FWFSHFTWFLHKRDRSTNEIIPKHRKWENATDLEKQTFYRFLHKTYLLHPIAVAVILYSI 180
           FWFSHFTWFLHKRDRS NEIIPKHRKWENATDLEKQTFYRFLHKTYLLHPIAVAVILYSI
Sbjct: 121 FWFSHFTWFLHKRDRSANEIIPKHRKWENATDLEKQTFYRFLHKTYLLHPIAVAVILYSI 180

Query: 181 GGTPFFIWGTCVRTVMIQHATFFVNSVCHMWGKRPWKTNDLSRNNWWVSLLLFGEGWHNN 240
           GGTPFFIWGTCVRTVMIQHATFFVNSVCHMWGKRPWKTNDLSRNNWWVSLLLFGEGWHNN
Sbjct: 181 GGTPFFIWGTCVRTVMIQHATFFVNSVCHMWGKRPWKTNDLSRNNWWVSLLLFGEGWHNN 240

Query: 241 HHAFEYSARMGIEWWQYDPGWDRSVNEMIPRRRKWENATDLEKQTFYRFLHKTYIYHPIA 300
           HHAFEYSARMGIEWWQY                                           
Sbjct: 241 HHAFEYSARMGIEWWQY------------------------------------------- 290

Query: 301 LAVILYSIGGAPFLIWGMCVRIVVGLHATFLVNSVSHMWGKKPWKTNDLSRNNWWISFLV 360
                                                                       
Sbjct: 301 ------------------------------------------------------------ 290

Query: 361 FGEGWHNNHHAFEYSARLGIEWWQYDPGWYVIRFLEAIGVATHVKLPSQTHMQKLAKD 395
                                    DPGWYVIRFL+AIGVATHVKLPSQTHMQKLA D
Sbjct: 361 -------------------------DPGWYVIRFLQAIGVATHVKLPSQTHMQKLAND 290

BLAST of Csor.00g134590 vs. ExPASy TrEMBL
Match: A0A6J1IZD1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111479850 PE=3 SV=1)

HSP 1 Score: 467 bits (1201), Expect = 1.48e-162
Identity = 241/418 (57.66%), Postives = 252/418 (60.29%), Query Frame = 0

Query: 1   MDASKEQNRGVKPMSFPKIKPPFVMFFIHFFC-----------------------LFGIT 60
           MDAS +QNR VKPMSFPKI   FV+FFIHFFC                       LFGIT
Sbjct: 1   MDASNKQNRVVKPMSFPKIIRLFVLFFIHFFCIFAPFQINWSAISVCFILYIITGLFGIT 60

Query: 61  ISYHRNLSHKSFKIPKLLDYLFAYCGVHALQGDPIFWVSTHRYHHQYVHTAKDPHSPIKG 120
           ISYHRNLSHKSFKI K L+YLFAYCGVHA QGDPIFWVSTHRYHHQYV T KDPHSPIKG
Sbjct: 61  ISYHRNLSHKSFKISKWLEYLFAYCGVHAFQGDPIFWVSTHRYHHQYVDTPKDPHSPIKG 120

Query: 121 FWFSHFTWFLHKRDRSTNEIIPKHRKWENATDLEKQTFYRFLHKTYLLHPIAVAVILYSI 180
           FWFSHFTWFLHK DR+ +E+IP+HRKWENATDLEKQTFYRFLHKTY+LHPIAVAVILYSI
Sbjct: 121 FWFSHFTWFLHKMDRTVDEMIPRHRKWENATDLEKQTFYRFLHKTYMLHPIAVAVILYSI 180

Query: 181 GGTPFFIWGTCVRTVMIQHATFFVNSVCHMWGKRPWKTNDLSRNNWWVSLLLFGEGWHNN 240
           GGTPFFIWG CVRTVMIQHATFFVNSVCHMWGK+PWKTNDLSRNNW VSLL+FGEGWHNN
Sbjct: 181 GGTPFFIWGMCVRTVMIQHATFFVNSVCHMWGKQPWKTNDLSRNNWVVSLLIFGEGWHNN 240

Query: 241 HHAFEYSARMGIEWWQYDPGWDRSVNEMIPRRRKWENATDLEKQTFYRFLHKTYIYHPIA 300
           HHAFEYSARMGIEWWQY                                           
Sbjct: 241 HHAFEYSARMGIEWWQY------------------------------------------- 290

Query: 301 LAVILYSIGGAPFLIWGMCVRIVVGLHATFLVNSVSHMWGKKPWKTNDLSRNNWWISFLV 360
                                                                       
Sbjct: 301 ------------------------------------------------------------ 290

Query: 361 FGEGWHNNHHAFEYSARLGIEWWQYDPGWYVIRFLEAIGVATHVKLPSQTHMQKLAKD 395
                                    DPGWYVIRFL+AIGVATHVKLPSQTHMQKLA D
Sbjct: 361 -------------------------DPGWYVIRFLQAIGVATHVKLPSQTHMQKLAND 290

BLAST of Csor.00g134590 vs. ExPASy TrEMBL
Match: A0A6J1GWI8 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111457792 PE=3 SV=1)

HSP 1 Score: 457 bits (1177), Expect = 8.82e-159
Identity = 244/426 (57.28%), Postives = 256/426 (60.09%), Query Frame = 0

Query: 1   MDASKEQNRGVKP-MSF-----PKIKPP--FVMFFIHFFC-------------------- 60
           MDAS+E  R VKP MSF     PKIK    F++FF+HFFC                    
Sbjct: 1   MDASREPKRIVKPPMSFGKRKWPKIKKKIVFILFFMHFFCIFAPFQVNLGAISICFALYN 60

Query: 61  ---LFGITISYHRNLSHKSFKIPKLLDYLFAYCGVHALQGDPIFWVSTHRYHHQYVHTAK 120
              LFG+TISYHRNL+HKSF +PK L+YLFAYCGVH+ QGDPI+WVSTHRYHHQYV T K
Sbjct: 61  ITGLFGLTISYHRNLTHKSFVLPKWLEYLFAYCGVHSFQGDPIYWVSTHRYHHQYVDTPK 120

Query: 121 DPHSPIKGFWFSHFTWFLHKRDRSTNEIIPKHRKWENATDLEKQTFYRFLHKTYLLHPIA 180
           DPHSPIKGFW+SH TW+LHKRDRS                                    
Sbjct: 121 DPHSPIKGFWYSHLTWYLHKRDRS------------------------------------ 180

Query: 181 VAVILYSIGGTPFFIWGTCVRTVMIQHATFFVNSVCHMWGKRPWKTNDLSRNNWWVSLLL 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 241 FGEGWHNNHHAFEYSARMGIEWWQYDPGWDRSVNEMIPRRRKWENATDLEKQTFYRFLHK 300
                                           VNEMIPRRRKWENATDLEKQTFYRFLHK
Sbjct: 241 --------------------------------VNEMIPRRRKWENATDLEKQTFYRFLHK 298

Query: 301 TYIYHPIALAVILYSIGGAPFLIWGMCVRIVVGLHATFLVNSVSHMWGKKPWKTNDLSRN 360
           TYIYHPIALAVILYSIGGAPFLIWGMCVRIVVGLHATFLVNSVSHMWGKKPWKTNDLSRN
Sbjct: 301 TYIYHPIALAVILYSIGGAPFLIWGMCVRIVVGLHATFLVNSVSHMWGKKPWKTNDLSRN 298

Query: 361 NWWISFLVFGEGWHNNHHAFEYSARLGIEWWQYDPGWYVIRFLEAIGVATHVKLPSQTHM 395
           NWWISFLVFGEGWHNNHHAFEYSARLGIEWWQYDPGWYVIRFLEAIGVATHVKLPSQTHM
Sbjct: 361 NWWISFLVFGEGWHNNHHAFEYSARLGIEWWQYDPGWYVIRFLEAIGVATHVKLPSQTHM 298

BLAST of Csor.00g134590 vs. ExPASy TrEMBL
Match: A0A6J1IRG4 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111479848 PE=3 SV=1)

HSP 1 Score: 446 bits (1147), Expect = 3.20e-154
Identity = 238/426 (55.87%), Postives = 251/426 (58.92%), Query Frame = 0

Query: 1   MDASKEQNRGVKP-MSFPKIKPP-------FVMFFIHFFC-------------------- 60
           MDAS+E  R VKP MSF K K P       F++F +HFFC                    
Sbjct: 1   MDASREPKRIVKPPMSFGKRKWPKINKKIVFILFLMHFFCIFAPYQVNLGAISMCFVLYN 60

Query: 61  ---LFGITISYHRNLSHKSFKIPKLLDYLFAYCGVHALQGDPIFWVSTHRYHHQYVHTAK 120
              LFG+TISYHRNL+HKSF +PK L+YLFAYCGVH+ QGDPI+WVSTHRYHHQYV T K
Sbjct: 61  ITGLFGLTISYHRNLTHKSFILPKWLEYLFAYCGVHSFQGDPIYWVSTHRYHHQYVDTLK 120

Query: 121 DPHSPIKGFWFSHFTWFLHKRDRSTNEIIPKHRKWENATDLEKQTFYRFLHKTYLLHPIA 180
           DPHSPIKGFW+SH TW+LHKRDRS                                    
Sbjct: 121 DPHSPIKGFWYSHLTWYLHKRDRS------------------------------------ 180

Query: 181 VAVILYSIGGTPFFIWGTCVRTVMIQHATFFVNSVCHMWGKRPWKTNDLSRNNWWVSLLL 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 241 FGEGWHNNHHAFEYSARMGIEWWQYDPGWDRSVNEMIPRRRKWENATDLEKQTFYRFLHK 300
                                           +NEMIPRRRKWENATDLEKQTFYRFLHK
Sbjct: 241 --------------------------------INEMIPRRRKWENATDLEKQTFYRFLHK 298

Query: 301 TYIYHPIALAVILYSIGGAPFLIWGMCVRIVVGLHATFLVNSVSHMWGKKPWKTNDLSRN 360
           TYIYHPIALAVILYSIGGAPFLIWGMCVRIVVGLHATFLVNSV HMWGKKPWKTNDLS N
Sbjct: 301 TYIYHPIALAVILYSIGGAPFLIWGMCVRIVVGLHATFLVNSVCHMWGKKPWKTNDLSTN 298

Query: 361 NWWISFLVFGEGWHNNHHAFEYSARLGIEWWQYDPGWYVIRFLEAIGVATHVKLPSQTHM 395
           NWWISFLVFGEGWHNNHHAFEYSARLGIEWWQYDPGWYVIRFLEAIGVATHVKLPSQT M
Sbjct: 361 NWWISFLVFGEGWHNNHHAFEYSARLGIEWWQYDPGWYVIRFLEAIGVATHVKLPSQTLM 298

BLAST of Csor.00g134590 vs. ExPASy TrEMBL
Match: A0A6J1GV23 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111457794 PE=3 SV=1)

HSP 1 Score: 421 bits (1083), Expect = 1.64e-144
Identity = 221/425 (52.00%), Postives = 240/425 (56.47%), Query Frame = 0

Query: 1   MDASKEQNRGVKPMSF-----PKIKPPFVM--FFIHFFCLF------------------- 60
           MDAS+EQN  VKP SF     PKIK  F+   FFIHF CLF                   
Sbjct: 1   MDASREQNIIVKPKSFWKRKWPKIKKKFIFVSFFIHFSCLFAPFQINLGAISICFVLYII 60

Query: 61  ----GITISYHRNLSHKSFKIPKLLDYLFAYCGVHALQGDPIFWVSTHRYHHQYVHTAKD 120
               GITISYHRNL+HK+FK+PK L+YL AYCG+HALQGDPI+WVS HRYHHQYV T  D
Sbjct: 61  TGLFGITISYHRNLTHKTFKLPKWLEYLLAYCGLHALQGDPIYWVSIHRYHHQYVDTPTD 120

Query: 121 PHSPIKGFWFSHFTWFLHKRDRSTNEIIPKHRKWENATDLEKQTFYRFLHKTYLLHPIAV 180
           PHSPIKGFWFSH TW+L KR++S +E+IP+HRKWEN TDLEKQTFYRFLHKTYLLHPI +
Sbjct: 121 PHSPIKGFWFSHVTWYLQKRNQSVSEMIPRHRKWENVTDLEKQTFYRFLHKTYLLHPITL 180

Query: 181 AVILYSIGGTPFFIWGTCVRTVMIQHATFFVNSVCHMWGKRPWKTNDLSRNNWWVSLLLF 240
           A ILYSIGG PFFIWG CVR V++ HATF VNSVCHMWGK+PWKTNDLSRNN W+SLL F
Sbjct: 181 AAILYSIGGAPFFIWGMCVRIVIVLHATFLVNSVCHMWGKQPWKTNDLSRNNGWISLLAF 240

Query: 241 GEGWHNNHHAFEYSARMGIEWWQYDPGWDRSVNEMIPRRRKWENATDLEKQTFYRFLHKT 300
           GEGWHNNHHAF                                                 
Sbjct: 241 GEGWHNNHHAF------------------------------------------------- 297

Query: 301 YIYHPIALAVILYSIGGAPFLIWGMCVRIVVGLHATFLVNSVSHMWGKKPWKTNDLSRNN 360
                                                                       
Sbjct: 301 ------------------------------------------------------------ 297

Query: 361 WWISFLVFGEGWHNNHHAFEYSARLGIEWWQYDPGWYVIRFLEAIGVATHVKLPSQTHMQ 395
                              EYSARLGIEWWQYDPGWYVIRFLEAIGVATHVKLPSQTHMQ
Sbjct: 361 -------------------EYSARLGIEWWQYDPGWYVIRFLEAIGVATHVKLPSQTHMQ 297

BLAST of Csor.00g134590 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 300.1 bits (767), Expect = 2.7e-81
Identity = 129/206 (62.62%), Postives = 155/206 (75.24%), Query Frame = 0

Query: 33  LFGITISYHRNLSHKSFKIPKLLDYLFAYCGVHALQGDPIFWVSTHRYHHQYVHTAKDPH 92
           L GIT+S+HRNLSHK+FK+PK L+YLFAYCG  ALQG+PI WVSTHRYHHQ+  + +DPH
Sbjct: 140 LLGITLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPH 199

Query: 93  SPIKGFWFSHFTWFLHKRDRSTNEIIPKHRKWENATDLEKQTFYRFLHKTYLLHPIAVAV 152
           SP+ GFWFSH  W        TN I  +  +  N  DLEKQ FYRFL  TY+LHP+A+AV
Sbjct: 200 SPLDGFWFSHMNWMF-----DTNTITQRCGEPNNVGDLEKQPFYRFLRTTYILHPLALAV 259

Query: 153 ILYSIGGTPFFIWGTCVRTVMIQHATFFVNSVCHMWGKRPWKTNDLSRNNWWVSLLLFGE 212
            LY++GG PF +WG  VR V + H T+ VNS CH+WGK+ W T DLS+NNWWV+ L FGE
Sbjct: 260 ALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAALAFGE 319

Query: 213 GWHNNHHAFEYSARMGIEWWQYDPGW 239
           GWHNNHHAFE+SAR G+EWWQ D  W
Sbjct: 320 GWHNNHHAFEFSARHGLEWWQLDMTW 340

BLAST of Csor.00g134590 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 266.9 bits (681), Expect = 2.5e-71
Identity = 119/214 (55.61%), Postives = 146/214 (68.22%), Query Frame = 0

Query: 25  MFFIHFFCLFGITISYHRNLSHKSFKIPKLLDYLFAYCGVHALQGDPIFWVSTHRYHHQY 84
           + FI+  C   IT+SYHRNLSH+SF +PK L+YLFAY GV A QGDPI WVS HRYHH++
Sbjct: 127 LVFINGIC---ITLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKH 186

Query: 85  VHTAKDPHSPIKGFWFSHFTWFLHKRDRSTNEIIPKHRKWENATDLEKQTFYRFLHKTYL 144
             T +DPHSP +GFWFSH  W        T+ I+      EN  DL +Q FYRFL +T L
Sbjct: 187 CETQRDPHSPTQGFWFSHMAWIF-----DTSSILENCGGEENVDDLVRQPFYRFLQRTVL 246

Query: 145 LHPIAVAVILYSIGGTPFFIWGTCVRTVMIQHATFFVNSVCHMWGKRPWKTNDLSRNNWW 204
           LH +A + + Y  GG P  +WG  +   +  H TF VNSVCH+WG R W T+D S+NNWW
Sbjct: 247 LHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFSKNNWW 306

Query: 205 VSLLLFGEGWHNNHHAFEYSARMGIEWWQYDPGW 239
           V++L  GEGWHNNHHAFE+SAR G+EWWQ D  W
Sbjct: 307 VAILTLGEGWHNNHHAFEFSARHGLEWWQLDITW 332

BLAST of Csor.00g134590 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 266.2 bits (679), Expect = 4.3e-71
Identity = 118/238 (49.58%), Postives = 147/238 (61.76%), Query Frame = 0

Query: 23  FVMFFIHFFCL----------------------FGITISYHRNLSHKSFKIPKLLDYLFA 82
           F   F+HF CL                       GIT+SYHRNL+H+SFK+PK L+Y FA
Sbjct: 43  FASLFVHFLCLLAPFNFTWPALRVALIVYTVGGLGITVSYHRNLAHRSFKVPKWLEYFFA 102

Query: 83  YCGVHALQGDPIFWVSTHRYHHQYVHTAKDPHSPIKGFWFSHFTWFLHKRDRSTNEIIPK 142
           YCG+ A+QGDPI WVSTHRYHHQ+  + +DPHSP +GFWFSH  W        T  ++ K
Sbjct: 103 YCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGFWFSHLLWLF-----DTGYLVEK 162

Query: 143 HRKWENATDLEKQTFYRFLHKTYLLHPIAVAVILYSIGGTPFFIWGTCVRTVMIQHATFF 202
             +  N  DL++Q +Y+FL +T L H +    +LY  GG  F  WG  +   M  H T  
Sbjct: 163 CGRRTNVEDLKRQWYYKFLQRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCL 222

Query: 203 VNSVCHMWGKRPWKTNDLSRNNWWVSLLLFGEGWHNNHHAFEYSARMGIEWWQYDPGW 239
           +NS+CH+WG R WKTND SRN WW+S+  FGE WHNNHHAFE SAR G+EWWQ D  W
Sbjct: 223 INSLCHVWGSRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISW 275

BLAST of Csor.00g134590 vs. TAIR 10
Match: AT2G31360.1 (16:0delta9 desaturase 2 )

HSP 1 Score: 259.6 bits (662), Expect = 4.0e-69
Identity = 114/216 (52.78%), Postives = 144/216 (66.67%), Query Frame = 0

Query: 23  FVMFFIHFFCLFGITISYHRNLSHKSFKIPKLLDYLFAYCGVHALQGDPIFWVSTHRYHH 82
           +V F  +     GIT+SYHRNL+H+SFK+PK L+YL AYC + A+QGDPI WVSTHRYHH
Sbjct: 67  WVTFLFYTIGGLGITVSYHRNLAHRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHH 126

Query: 83  QYVHTAKDPHSPIKGFWFSHFTWFLHKRDRSTNEIIPKHRKWENATDLEKQTFYRFLHKT 142
           Q+  + +DPHSP +GFWFSH  W        +  ++ K  +  N  DL++Q FYRFL KT
Sbjct: 127 QFTDSERDPHSPKEGFWFSHLLWIY-----DSAYLVSKCGRRANVEDLKRQWFYRFLQKT 186

Query: 143 YLLHPIAVAVILYSIGGTPFFIWGTCVRTVMIQHATFFVNSVCHMWGKRPWKTNDLSRNN 202
            L H + +   L+ +GG  F  WG  V   +  H T  +NS+CH+WG R WKTND SRN 
Sbjct: 187 VLFHILGLGFFLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNV 246

Query: 203 WWVSLLLFGEGWHNNHHAFEYSARMGIEWWQYDPGW 239
           WW+S+  FGE WHNNHHAFE SAR G+EWWQ D  W
Sbjct: 247 WWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISW 277

BLAST of Csor.00g134590 vs. TAIR 10
Match: AT1G06100.1 (Fatty acid desaturase family protein )

HSP 1 Score: 238.0 bits (606), Expect = 1.3e-62
Identity = 108/203 (53.20%), Postives = 137/203 (67.49%), Query Frame = 0

Query: 36  ITISYHRNLSHKSFKIPKLLDYLFAYCGVHALQGDPIFWVSTHRYHHQYVHTAKDPHSPI 95
           IT SYHRNL+H+SFK+PK L+Y FAY  + ALQGDP+ WVS HR+HHQ+  + +DPHSPI
Sbjct: 72  ITFSYHRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDWVSIHRFHHQFTDSDRDPHSPI 131

Query: 96  KGFWFSHFTWFLHKRDRSTNEIIPKHRKWENATDLEKQTFYRFLHKTYLLHPIAVAVILY 155
           +GFWFSH  W        T+ I  K  +  N  DL++Q FYRFL KT +LH +A   ++Y
Sbjct: 132 EGFWFSHVLWIF-----DTDYIREKCGRRNNVMDLKQQWFYRFLKKTLVLHILAFWTLIY 191

Query: 156 SIGGTPFFIWGTCVRTVMIQHATFFVNSVCHMWGKRPWKTNDLSRNNWWVSLLLFGEGWH 215
             GG P+  W      V+  H T+ VNS CH+ G + W+TND SRN WW++LL  GE WH
Sbjct: 192 LWGGLPYLTWTVGFGGVIGYHGTWLVNSACHICGSQAWQTNDTSRNVWWLALLTMGESWH 251

Query: 216 NNHHAFEYSARMGIEWWQYDPGW 239
           NNHHAFE SAR G+EW+Q D  W
Sbjct: 252 NNHHAFETSARHGLEWYQLDITW 269

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q949X03.8e-8062.62Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab... [more]
P0DOW23.3e-7657.60Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1[more]
P0DOW39.1e-7458.25Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1[more]
Q9LVZ33.6e-7055.61Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
O657976.1e-7049.58Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
KAG6581936.10.0100.00Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic, partia... [more]
KAG6581935.12.72e-21060.28Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic, partia... [more]
XP_022955965.11.39e-17962.44palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C... [more]
XP_023529140.14.74e-17061.48palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C... [more]
XP_022980469.13.06e-16257.66palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C... [more]
Match NameE-valueIdentityDescription
A0A6J1GWJ36.74e-18062.44palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1IZD11.48e-16257.66palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1GWI88.82e-15957.28palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1IRG43.20e-15455.87palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1GV231.64e-14452.00palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
Match NameE-valueIdentityDescription
AT3G15850.12.7e-8162.62fatty acid desaturase 5 [more]
AT3G15870.12.5e-7155.61Fatty acid desaturase family protein [more]
AT1G06080.14.3e-7149.58delta 9 desaturase 1 [more]
AT2G31360.14.0e-6952.7816:0delta9 desaturase 2 [more]
AT1G06100.11.3e-6253.20Fatty acid desaturase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015876Acyl-CoA desaturasePRINTSPR00075FACDDSATRASEcoord: 41..61
score: 31.83
coord: 78..107
score: 50.35
coord: 168..189
score: 40.43
coord: 211..225
score: 60.7
coord: 18..40
score: 21.51
IPR015876Acyl-CoA desaturasePANTHERPTHR11351ACYL-COA DESATURASEcoord: 23..238
IPR015876Acyl-CoA desaturasePANTHERPTHR11351ACYL-COA DESATURASEcoord: 242..393
IPR015876Acyl-CoA desaturaseCDDcd03505Delta9-FADS-likecoord: 23..238
e-value: 5.14299E-61
score: 193.156
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 26..247
e-value: 3.2E-17
score: 63.2
coord: 268..377
e-value: 3.4E-11
score: 43.5
NoneNo IPR availablePANTHERPTHR11351:SF94BNAC05G37460D PROTEINcoord: 23..238
coord: 242..393
NoneNo IPR availableCDDcd01060Membrane-FADS-likecoord: 307..349
e-value: 3.69146E-5
score: 40.9162

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g134590.m01Csor.00g134590.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006631 fatty acid metabolic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016020 membrane
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water