Csor.00g134260 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g134260
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	EXPERA domain-containing protein
Location	Csor_Chr14: 12889884 .. 12892330 (-)
RNA-Seq Expression	Csor.00g134260
Synteny	Csor.00g134260

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGGGGAATCTGAAGCTGCTCGGCATCCCTACGCACCCACAGATCTGGAACTCCCCGGATATGTTCCTATTTCACTACCCCTCACTACTATTCTCGGCGTTTATGTCCTCTCTTCTCTCTTTATCACTTTGCTGCTCTGGACTCTTTCCGGTATGCTTTCATCTCTTACTTTGATATTTACTGTCGTTTTTTTGGATTCTGTTTTATGCGCTTCGAATTCTGCTTGATTTCTTGCTGGAGAAGAAGACCGATGGTTTTCATAATCCGTAGTTTGTTGTTGTGTTGGATTTCTGTCCACTTGTTGAGTGAAGTAATTGGAAGCAAAATCAAACAATTTCAAATATTCTATGTTTTTCGTGTCCTCCAAGAATTCTATTGAATGTCTTTGTTGAATCTGAAGGCTGGCGGTGAAACCGATGTAAAAATTTAAATCTGTCTCGTTCAATTATTCTTTTCGAGAGGATCGAGATCTGTTAAACGGATAGACGCCAGATTTCCCTTGCCTTGCCTTCCTACTCCTTAACTTGTGCTATTGTCTTTTGGTTTAGATGATTGCTTTAGAAAGGGAATACTGATGGGACGGACTAAGCGAGGATGTCTATGATTATACCTGAGTGTGAAAGAATGAAACATGTAGCAAACTGAAATCTATTTGGCCATTTATGGTTCTGTCAATCCGCGTTTGACTTCATTTGATTGGTTAATTTTGACACGATACAATATCTATTTTAGATTTAAACTCTTGATGTTTCCTTTGGTTATGCATACTCCCCTTTGTCTAAACAATCATTCATCCATACACTTTCTACTAATGAGGAACATAAATTTATCTAGATTTCAGAATGCTATGAGGATGAAATGTTAATGTTAAGATTACTATTTGGATATCAGTGATTAGCTTGTAAGTTTGTTTGTGTTGAGTGGTTATGGATATAGTGGGTTGAGAGAGTGAAAGAGAGCCAGATTTGTATAGTTATGGAAAGAGTACTAGGCCTCTCGTAGAGCTTGGAACCATGTAATTTGTCTAATATCAATATAATATTTCAATTAACCTGTCCAATTTTTGGTTCCTATCAAATTAGTTCCACTACATTTGTGCTCGAAATTGAATCTTTTCAAGTCTAGAAGAATATTGATGAACATGTTGTTCACAGATGGATCTTTTTCTTTTTAGGGCAGATAGTAGGGATGTTCATTTTGGGGTCCTTGTCCTTCGAAGTGTTTTTTCTTGTAGCTTGAGCCATCCCTCTCCAATGGTTCCACTTTTTTCCTTAGTGTAGGTAGAGTGTTGTACAAACACTAGATCCTAACGCCATGATTGAAGTATGAAACACCTGATAATGGGCACTATACTATATTCAAACACTTAGTGTTGGTAGAGTGTTGTACAAGCACTTATGTTGCAAGCTATGACATATTTTGCTTTCCTATTGCACCCTGTGTTTTTTTCATGTTCCCTTCCGTGGTGTTTGTACACTTGTTCTCTCCATCTTTATCTATTTCTTTTTATCAGAATTTAGTAATGGAAAAAACTGTAGTTCTTAGAGTTGTTTGGAAAAGGTTCTATGCTTGTTGCTTTATCTTCATTTCCCCCTTATAACAGGACGACTTCCCAAAAAAACAAAGATTGAGAGGCTGCTCATGTGCTGGTGGATATTTACGGGCCTTACCCACATTGTTCTCGAGGGTTACTTTGTTTTCTCTCCAGAATTTTACAAGGACAGTTCTTACCTTGCTGAAGTTTGTAAGTTAATTTAATTCACTTTCTTTGTTACCCTAGCTGTGGTATGGAAGGGTTGTATGGATGCTCCTCTAATAATGTTCATGTGCACTACTTACCAGGGAAAGAATACAGCAAGGGAGATTCTCGATACGCAACTCGGGACTCTGCGATTGTCAGTCTTGAAGGACTGACTGCTGTTTTAGAGGGTCCAGCTTGCATCTTAGCAGTGTAAGAATTTATCATCTCTGTGGTTTTGGGAGAGAATAGTTAATCAATTTCTAAATGACTAAGATACAAGAAGTTATGATACAAAACAAATACAAAAGAGGTAACTAGCTAATAAAATCTAAACTAAAGAATTTTTAAATGCTATACATATAAGGAATTACATTTGAACTTTCTGTTCATAATTAGCCATCCATCAACAAATTTGTTGGGCTGTGTATAATTCGCAGGTATGCAATAGCTACAGACAAGTCGTACAGTTACGTACTGCAGTTCGCCATATCATTGGGACAGCTATATGGAACTTTCATGTACTACATAACTGCTCTGTTGGACGGTGATAATTTTTCTTCATCCCCAATTTATTACTATTTATACTACGTATTGTTAAATTCCTTCTGGATTCTTATTCCTTCGCTTATTGCTATCCGGTGTTGGAAGAAAATTTGTATTGCTATGAGCAGTGAAAGCCACAAGAAAACCAAAGTTCCCTAA

mRNA sequence

ATGGCGGGGGAATCTGAAGCTGCTCGGCATCCCTACGCACCCACAGATCTGGAACTCCCCGGATATGTTCCTATTTCACTACCCCTCACTACTATTCTCGGCGTTTATGTCCTCTCTTCTCTCTTTATCACTTTGCTGCTCTGGACTCTTTCCGGACGACTTCCCAAAAAAACAAAGATTGAGAGGCTGCTCATGTGCTGGTGGATATTTACGGGCCTTACCCACATTGTTCTCGAGGGTTACTTTGTTTTCTCTCCAGAATTTTACAAGGACAGTTCTTACCTTGCTGAAGTTTGGAAAGAATACAGCAAGGGAGATTCTCGATACGCAACTCGGGACTCTGCGATTGTCAGTCTTGAAGGACTGACTGCTGTTTTAGAGGGTCCAGCTTGCATCTTAGCAGTGTATGCAATAGCTACAGACAAGTCGTACAGTTACGTACTGCAGTTCGCCATATCATTGGGACAGCTATATGGAACTTTCATGTACTACATAACTGCTCTGTTGGACGGTGATAATTTTTCTTCATCCCCAATTTATTACTATTTATACTACGTATTGTTAAATTCCTTCTGGATTCTTATTCCTTCGCTTATTGCTATCCGGTGTTGGAAGAAAATTTGTATTGCTATGAGCAGTGAAAGCCACAAGAAAACCAAAGTTCCCTAA

Coding sequence (CDS)

ATGGCGGGGGAATCTGAAGCTGCTCGGCATCCCTACGCACCCACAGATCTGGAACTCCCCGGATATGTTCCTATTTCACTACCCCTCACTACTATTCTCGGCGTTTATGTCCTCTCTTCTCTCTTTATCACTTTGCTGCTCTGGACTCTTTCCGGACGACTTCCCAAAAAAACAAAGATTGAGAGGCTGCTCATGTGCTGGTGGATATTTACGGGCCTTACCCACATTGTTCTCGAGGGTTACTTTGTTTTCTCTCCAGAATTTTACAAGGACAGTTCTTACCTTGCTGAAGTTTGGAAAGAATACAGCAAGGGAGATTCTCGATACGCAACTCGGGACTCTGCGATTGTCAGTCTTGAAGGACTGACTGCTGTTTTAGAGGGTCCAGCTTGCATCTTAGCAGTGTATGCAATAGCTACAGACAAGTCGTACAGTTACGTACTGCAGTTCGCCATATCATTGGGACAGCTATATGGAACTTTCATGTACTACATAACTGCTCTGTTGGACGGTGATAATTTTTCTTCATCCCCAATTTATTACTATTTATACTACGTATTGTTAAATTCCTTCTGGATTCTTATTCCTTCGCTTATTGCTATCCGGTGTTGGAAGAAAATTTGTATTGCTATGAGCAGTGAAAGCCACAAGAAAACCAAAGTTCCCTAA

Protein sequence

MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKIERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYATRDSAIVSLEGLTAVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSPIYYYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP

Homology

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BLAST of Csor.00g134260 vs. ExPASy Swiss-Prot
Match: O48962 (Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Arabidopsis thaliana OX=3702 GN=At1g20050 PE=2 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 1.3e-74
Identity = 138/224 (61.61%), Postives = 178/224 (79.46%), Query Frame = 0


Query: 6   EAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKIERLLM 65
           E   HPY P DL LPGYVPIS+ +++I+ +Y+ SSL +  L+W L GR  KK K+++LLM
Sbjct: 2   EELAHPYVPRDLNLPGYVPISMSMSSIVSIYLGSSLLVVSLVWLLFGR--KKAKLDKLLM 61

Query: 66  CWWIFTGLTHIVLEGYFVFSPEFYKD--SSYLAEVWKEYSKGDSRYATRDSAIVSLEGLT 125
           CWW FTGLTH++LEGYFVFSPEF+KD  S+YLAEVWKEYSKGDSRY  RDSA+VS+EG+T
Sbjct: 62  CWWTFTGLTHVILEGYFVFSPEFFKDNTSAYLAEVWKEYSKGDSRYVGRDSAVVSVEGIT 121

Query: 126 AVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSPIYYYL 185
           AV+ GPA +LA+YAIA +KSYSYVLQ AIS+ QLYG  +Y+ITA+L+GDNF+++  YYY 
Sbjct: 122 AVIVGPASLLAIYAIAKEKSYSYVLQLAISVCQLYGCLVYFITAILEGDNFATNSFYYYS 181

Query: 186 YYVLLNSFWILIPSLIAIRCWKKICIA-------MSSESHKKTK 221
           YY+  N +W+LIPSLI+ RCWKKIC A       + +++ KKT+
Sbjct: 182 YYIGANCWWVLIPSLISFRCWKKICAAAAIANNNVETKTKKKTR 223

[Close]

BLAST of Csor.00g134260 vs. ExPASy Swiss-Prot
Match: Q9FTZ2 (Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0103600 PE=2 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 9.6e-65
Identity = 122/211 (57.82%), Postives = 159/211 (75.36%), Query Frame = 0


Query: 10  HPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKK-TKIERLLMCWW 69
           HPYAP +L LPG+VP+ L    IL  Y+ +SLF+ L +W +SGR  ++ +  +R LMCWW
Sbjct: 7   HPYAPAELHLPGFVPLQLSQAQILVPYLATSLFLLLAVWLISGRCSRRLSDTDRWLMCWW 66

Query: 70  IFTGLTHIVLEGYFVFSPEFY--KDSSYLAEVWKEYSKGDSRYATRDSAIVSLEGLTAVL 129
            FTGLTHI++EG FVF+P F+  ++ SY  EVWKEYSKGDSRY  RD A V++EG+TAVL
Sbjct: 67  AFTGLTHIIIEGTFVFAPNFFSNQNPSYFDEVWKEYSKGDSRYVARDPATVTVEGITAVL 126

Query: 130 EGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSPIYYYLYYV 189
           EGPA +LAVYAIA+ KSYS++LQF + LGQLYG  +Y+ITA LDG NF +SP Y++ Y++
Sbjct: 127 EGPASLLAVYAIASGKSYSHILQFTVCLGQLYGCLVYFITAYLDGFNFWTSPFYFWAYFI 186

Query: 190 LLNSFWILIPSLIAIRCWKKICIAMSSESHK 218
             NS W++IP++IAIR WKKIC A   E  K
Sbjct: 187 GANSSWVVIPTMIAIRSWKKICAAFQGEKVK 217

[Close]

BLAST of Csor.00g134260 vs. ExPASy Swiss-Prot
Match: Q9JJ46 (3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Rattus norvegicus OX=10116 GN=Ebp PE=1 SV=3)

HSP 1 Score: 138.3 bits (347), Expect = 1.1e-31
Identity = 82/219 (37.44%), Postives = 126/219 (57.53%), Query Frame = 0


Query: 10  HPYAPTDLELPGYVPISLPLTTIL-GVYVLSSLFITLLLWTLSGR---LPKKTKIERLLM 69
           HPY P  L L  +VP  LP   IL G++  S + I ++ W LS R   +P  T   RL +
Sbjct: 9   HPYWPRHLRLDNFVPNDLPTWHILVGLFSFSGVLI-VITWLLSSRVSVVPLGTG-RRLAL 68

Query: 70  CWWIFTGLTHIVLEGYFVFSPE-FYKDSSYLAEVWKEYSKGDSRYATRDSAIVSLEGLTA 129
           CW+      H+V+EG+F F  E   +D ++L+++WKEYSKGDSRY   D  IV +E +TA
Sbjct: 69  CWFAVCTFIHLVIEGWFSFYHEILLEDQAFLSQLWKEYSKGDSRYILSDGFIVCMESVTA 128

Query: 130 VLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFS--SSPIYYY 189
            L GP  +  V A    + + +VLQ  +S+GQ+YG  +Y++T L DG        P+Y++
Sbjct: 129 CLWGPLSLWVVIAFLRHQPFRFVLQLVVSVGQIYGDVLYFLTELRDGFQHGELGHPLYFW 188

Query: 190 LYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKV 222
            Y+V++N+ W++IP ++     K +  A S   +K  K+
Sbjct: 189 FYFVIMNAIWLVIPGILVFDAIKHLTNAQSMLDNKVMKI 225

[Close]

BLAST of Csor.00g134260 vs. ExPASy Swiss-Prot
Match: P70245 (3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Mus musculus OX=10090 GN=Ebp PE=1 SV=3)

HSP 1 Score: 135.2 bits (339), Expect = 9.1e-31
Identity = 79/218 (36.24%), Postives = 128/218 (58.72%), Query Frame = 0


Query: 10  HPYAPTDLELPGYVPISLPLTTIL-GVYVLSSLFITLLLWTLSGR--LPKKTKIERLLMC 69
           HPY P  L+L  +VP  LP + IL G++ +S   I ++ W LS R  +       RL +C
Sbjct: 9   HPYWPRHLKLDNFVPNDLPTSHILVGLFSISGGLI-VITWLLSSRASVVPLGAGRRLALC 68

Query: 70  WWIFTGLTHIVLEGYF-VFSPEFYKDSSYLAEVWKEYSKGDSRYATRDSAIVSLEGLTAV 129
           W+      H+V+EG+F +++    +D ++L+++WKEYSKGDSRY   DS +V +E +TA 
Sbjct: 69  WFAVCTFIHLVIEGWFSLYNGILLEDQAFLSQLWKEYSKGDSRYILSDSFVVCMETVTAC 128

Query: 130 LEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFS--SSPIYYYL 189
           L GP  +  V A    + + +VLQ  +S+GQ+YG  +Y++T L +G        P+Y++ 
Sbjct: 129 LWGPLSLWVVIAFLRQQPFRFVLQLVVSMGQIYGDVLYFLTELHEGLQHGEIGHPVYFWF 188

Query: 190 YYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKV 222
           Y+V LN+ W++IPS++ +   K +  A S    K  K+
Sbjct: 189 YFVFLNAVWLVIPSILVLDAIKHLTSAQSVLDSKVMKI 225

[Close]

BLAST of Csor.00g134260 vs. ExPASy Swiss-Prot
Match: Q60490 (3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cavia porcellus OX=10141 GN=EBP PE=1 SV=3)

HSP 1 Score: 129.0 bits (323), Expect = 6.5e-29
Identity = 77/205 (37.56%), Postives = 112/205 (54.63%), Query Frame = 0


Query: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGR---LPKK 60
           MA  S    HPY P  L L  +VP  L    I+ V       + + +W LS R   +P  
Sbjct: 1   MATTSTGPLHPYWPRHLRLDHFVPNDLSAWYIVTVLFTVFGALVVTMWLLSSRASVVPLG 60

Query: 61  TKIERLLMCWWIFTGLTHIVLEGYFV-FSPEFYKDSSYLAEVWKEYSKGDSRYATRDSAI 120
           T   RL +CW+      H+V+EG+FV +      D ++L+++WKEY+KGDSRY   D+ I
Sbjct: 61  T-WRRLSVCWFAVCAFVHLVIEGWFVLYQKAILGDQAFLSQLWKEYAKGDSRYIIEDNFI 120

Query: 121 VSLEGLTAVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFS- 180
           + +E +T VL GP  + AV A        YVLQF ISLGQ+YG  +Y++T   DG     
Sbjct: 121 ICMESITVVLWGPLSLWAVIAFLRQHPSRYVLQFVISLGQIYGDLLYFLTEYRDGFQHGE 180

Query: 181 -SSPIYYYLYYVLLNSFWILIPSLI 200
              PIY++ Y+  +N  W++IP ++
Sbjct: 181 MGHPIYFWFYFFFMNVLWLVIPGVL 204

[Close]

BLAST of Csor.00g134260 vs. NCBI nr
Match: KAG6581969.1 (putative 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018388.1 putative 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 438 bits (1126), Expect = 7.97e-155
Identity = 222/222 (100.00%), Postives = 222/222 (100.00%), Query Frame = 0


Query: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKI 60
           MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKI
Sbjct: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKI 60

Query: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYATRDSAIVSLE 120
           ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYATRDSAIVSLE
Sbjct: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYATRDSAIVSLE 120

Query: 121 GLTAVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSPIY 180
           GLTAVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSPIY
Sbjct: 121 GLTAVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSPIY 180

Query: 181 YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP 222
           YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP
Sbjct: 181 YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP 222

[Close]

BLAST of Csor.00g134260 vs. NCBI nr
Match: XP_022955667.1 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucurbita moschata])

HSP 1 Score: 436 bits (1122), Expect = 3.25e-154
Identity = 221/222 (99.55%), Postives = 221/222 (99.55%), Query Frame = 0


Query: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKI 60
           MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKI
Sbjct: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKI 60

Query: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYATRDSAIVSLE 120
           ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKD SYLAEVWKEYSKGDSRYATRDSAIVSLE
Sbjct: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDGSYLAEVWKEYSKGDSRYATRDSAIVSLE 120

Query: 121 GLTAVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSPIY 180
           GLTAVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSPIY
Sbjct: 121 GLTAVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSPIY 180

Query: 181 YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP 222
           YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP
Sbjct: 181 YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP 222

[Close]

BLAST of Csor.00g134260 vs. NCBI nr
Match: XP_023526321.1 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 436 bits (1122), Expect = 3.25e-154
Identity = 220/222 (99.10%), Postives = 222/222 (100.00%), Query Frame = 0


Query: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKI 60
           MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVY+LSSLFITLLLWTLSGRLPKKTKI
Sbjct: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYLLSSLFITLLLWTLSGRLPKKTKI 60

Query: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYATRDSAIVSLE 120
           ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYATRDSAIVSLE
Sbjct: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYATRDSAIVSLE 120

Query: 121 GLTAVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSPIY 180
           GLTAVLEGPACILAVYAIATDKSYSY+LQFAISLGQLYGTFMYYITALLDGDNFSSSPIY
Sbjct: 121 GLTAVLEGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFSSSPIY 180

Query: 181 YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP 222
           YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP
Sbjct: 181 YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP 222

[Close]

BLAST of Csor.00g134260 vs. NCBI nr
Match: XP_022980063.1 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucurbita maxima])

HSP 1 Score: 433 bits (1113), Expect = 7.65e-153
Identity = 218/222 (98.20%), Postives = 221/222 (99.55%), Query Frame = 0


Query: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKI 60
           MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVY+LSSLFITLLLWTLSGRLPKKTKI
Sbjct: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYLLSSLFITLLLWTLSGRLPKKTKI 60

Query: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYATRDSAIVSLE 120
           ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYA R+SAIVSLE
Sbjct: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYAARESAIVSLE 120

Query: 121 GLTAVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSPIY 180
           GLTAVLEGPACILAVYAIATDKSYSY+LQFAISLGQLYGTFMYYITALLDGDNFSSSPIY
Sbjct: 121 GLTAVLEGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFSSSPIY 180

Query: 181 YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP 222
           YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP
Sbjct: 181 YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP 222

[Close]

BLAST of Csor.00g134260 vs. NCBI nr
Match: XP_038881035.1 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Benincasa hispida])

HSP 1 Score: 382 bits (980), Expect = 1.54e-132
Identity = 190/224 (84.82%), Postives = 207/224 (92.41%), Query Frame = 0


Query: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKI 60
           MAGESEAARHPY PTDL+LP YVPISLP++TIL VY+LSSLF+T L+WTLSGRL K+ KI
Sbjct: 1   MAGESEAARHPYIPTDLKLPEYVPISLPVSTILSVYLLSSLFVTFLVWTLSGRLTKRAKI 60

Query: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKD--SSYLAEVWKEYSKGDSRYATRDSAIVS 120
           ERLLMCWWIFTGLTH+VLEGYFVFSPEFYKD  S YLAEVWKEYSKGDSRYA R+S IVS
Sbjct: 61  ERLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120

Query: 121 LEGLTAVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSP 180
           +EGLT+VLEGPACILA YAIAT+KSYSY+LQFAISLGQLYGTF+YYITALLDGDNFSS P
Sbjct: 121 IEGLTSVLEGPACILAAYAIATEKSYSYILQFAISLGQLYGTFVYYITALLDGDNFSSGP 180

Query: 181 IYYYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP 222
           IYYY Y+V+ NSFWILIPSLIA RCWKKICIAMS+ESH KTKVP
Sbjct: 181 IYYYSYFVVANSFWILIPSLIATRCWKKICIAMSTESHMKTKVP 224

[Close]

BLAST of Csor.00g134260 vs. ExPASy TrEMBL
Match: A0A6J1GU98 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cucurbita moschata OX=3662 GN=LOC111457598 PE=3 SV=1)

HSP 1 Score: 436 bits (1122), Expect = 1.57e-154
Identity = 221/222 (99.55%), Postives = 221/222 (99.55%), Query Frame = 0


Query: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKI 60
           MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKI
Sbjct: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKI 60

Query: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYATRDSAIVSLE 120
           ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKD SYLAEVWKEYSKGDSRYATRDSAIVSLE
Sbjct: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDGSYLAEVWKEYSKGDSRYATRDSAIVSLE 120

Query: 121 GLTAVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSPIY 180
           GLTAVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSPIY
Sbjct: 121 GLTAVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSPIY 180

Query: 181 YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP 222
           YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP
Sbjct: 181 YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP 222

[Close]

BLAST of Csor.00g134260 vs. ExPASy TrEMBL
Match: A0A6J1IY56 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cucurbita maxima OX=3661 GN=LOC111479580 PE=3 SV=1)

HSP 1 Score: 433 bits (1113), Expect = 3.70e-153
Identity = 218/222 (98.20%), Postives = 221/222 (99.55%), Query Frame = 0


Query: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKI 60
           MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVY+LSSLFITLLLWTLSGRLPKKTKI
Sbjct: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYLLSSLFITLLLWTLSGRLPKKTKI 60

Query: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYATRDSAIVSLE 120
           ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYA R+SAIVSLE
Sbjct: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYAARESAIVSLE 120

Query: 121 GLTAVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSPIY 180
           GLTAVLEGPACILAVYAIATDKSYSY+LQFAISLGQLYGTFMYYITALLDGDNFSSSPIY
Sbjct: 121 GLTAVLEGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFSSSPIY 180

Query: 181 YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP 222
           YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP
Sbjct: 181 YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP 222

[Close]

BLAST of Csor.00g134260 vs. ExPASy TrEMBL
Match: A0A6J1E0U0 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Momordica charantia OX=3673 GN=LOC111024900 PE=3 SV=1)

HSP 1 Score: 368 bits (945), Expect = 1.60e-127
Identity = 179/223 (80.27%), Postives = 201/223 (90.13%), Query Frame = 0


Query: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKI 60
           MAG+SEAA HPY P DL+L  YVP+SLP +TI+GVY LSS+ +T L+WTLSGRLPKK KI
Sbjct: 1   MAGKSEAAPHPYVPADLQLSEYVPVSLPFSTIIGVYFLSSILVTSLVWTLSGRLPKKAKI 60

Query: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKD--SSYLAEVWKEYSKGDSRYATRDSAIVS 120
           ERLLMCWW+FTGLTH++LEGYFVFSPEFYKD  S YLAEVWKEYSKGDSRYA R+S IVS
Sbjct: 61  ERLLMCWWVFTGLTHMILEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120

Query: 121 LEGLTAVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSP 180
           +EGLT+VLEGPACILA+YAIAT+KSYSY+LQFAISLGQLYGTF+YYITA+LDGDNFSS P
Sbjct: 121 IEGLTSVLEGPACILAMYAIATEKSYSYILQFAISLGQLYGTFLYYITAVLDGDNFSSGP 180

Query: 181 IYYYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKV 221
            YYY YYV+ NSFWILIPSLIAIRCWKKIC AM+SE HKK K+
Sbjct: 181 FYYYAYYVVANSFWILIPSLIAIRCWKKICAAMNSEGHKKRKI 223

[Close]

BLAST of Csor.00g134260 vs. ExPASy TrEMBL
Match: A0A1S3BY31 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cucumis melo OX=3656 GN=LOC103494647 PE=3 SV=1)

HSP 1 Score: 363 bits (931), Expect = 2.18e-125
Identity = 183/222 (82.43%), Postives = 199/222 (89.64%), Query Frame = 0


Query: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKI 60
           MAG SEAA HPY PTDL+LP YVPISLPL+TILG Y+LSSLF+ LLLWTLSGRL KKTKI
Sbjct: 1   MAGASEAAPHPYVPTDLKLPDYVPISLPLSTILGGYLLSSLFLVLLLWTLSGRLTKKTKI 60

Query: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKD--SSYLAEVWKEYSKGDSRYATRDSAIVS 120
           +RLLMCWWIFTGLTH+VLEGYFVFSPEFYKD  S YLAEVWKEYSKGDSRYA R+S IV 
Sbjct: 61  DRLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVG 120

Query: 121 LEGLTAVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSP 180
           +EGLT+VLEGPACILA YAIA+ KSYSY+LQFAISLGQLYGT +YYITA+LDGD+FSS P
Sbjct: 121 IEGLTSVLEGPACILAAYAIASGKSYSYILQFAISLGQLYGTLLYYITAVLDGDDFSSGP 180

Query: 181 IYYYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTK 220
           IYYY YYV+ NSFW+LIPSLIAIRCWKKIC AMS ES  KTK
Sbjct: 181 IYYYSYYVVANSFWLLIPSLIAIRCWKKICNAMSIESQMKTK 222

[Close]

BLAST of Csor.00g134260 vs. ExPASy TrEMBL
Match: A0A0A0KVP4 (EXPERA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G007050 PE=3 SV=1)

HSP 1 Score: 360 bits (925), Expect = 1.79e-124
Identity = 180/222 (81.08%), Postives = 198/222 (89.19%), Query Frame = 0


Query: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKI 60
           MAG SEAA HPY PTDL+LP YVPISLPL+TILG Y+L+SLF+ LL+WTLSGRL KK KI
Sbjct: 1   MAGASEAAPHPYVPTDLKLPDYVPISLPLSTILGGYLLTSLFLVLLVWTLSGRLTKKAKI 60

Query: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKD--SSYLAEVWKEYSKGDSRYATRDSAIVS 120
           +RLLMCWWIFTGLTH+VLEGYFVFSPEFYKD  S YLAEVWKEYSKGDSRYA R+S IVS
Sbjct: 61  DRLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120

Query: 121 LEGLTAVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSP 180
           +EGLT+VLEGPACILA YAIA+ KSYSY+LQFAISLGQLYGT +YY+TA+LDGD+FSS P
Sbjct: 121 IEGLTSVLEGPACILAAYAIASGKSYSYILQFAISLGQLYGTLLYYVTAVLDGDDFSSGP 180

Query: 181 IYYYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTK 220
            YYY YYV+ NSFWILIPSLI IRCWKKICIAMS ES  KTK
Sbjct: 181 FYYYAYYVVANSFWILIPSLIGIRCWKKICIAMSIESQTKTK 222

[Close]

BLAST of Csor.00g134260 vs. TAIR 10
Match: AT1G20050.1 (C-8,7 sterol isomerase )

HSP 1 Score: 280.8 bits (717), Expect = 9.5e-76
Identity = 138/224 (61.61%), Postives = 178/224 (79.46%), Query Frame = 0


Query: 6   EAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKIERLLM 65
           E   HPY P DL LPGYVPIS+ +++I+ +Y+ SSL +  L+W L GR  KK K+++LLM
Sbjct: 2   EELAHPYVPRDLNLPGYVPISMSMSSIVSIYLGSSLLVVSLVWLLFGR--KKAKLDKLLM 61

Query: 66  CWWIFTGLTHIVLEGYFVFSPEFYKD--SSYLAEVWKEYSKGDSRYATRDSAIVSLEGLT 125
           CWW FTGLTH++LEGYFVFSPEF+KD  S+YLAEVWKEYSKGDSRY  RDSA+VS+EG+T
Sbjct: 62  CWWTFTGLTHVILEGYFVFSPEFFKDNTSAYLAEVWKEYSKGDSRYVGRDSAVVSVEGIT 121

Query: 126 AVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSPIYYYL 185
           AV+ GPA +LA+YAIA +KSYSYVLQ AIS+ QLYG  +Y+ITA+L+GDNF+++  YYY 
Sbjct: 122 AVIVGPASLLAIYAIAKEKSYSYVLQLAISVCQLYGCLVYFITAILEGDNFATNSFYYYS 181

Query: 186 YYVLLNSFWILIPSLIAIRCWKKICIA-------MSSESHKKTK 221
           YY+  N +W+LIPSLI+ RCWKKIC A       + +++ KKT+
Sbjct: 182 YYIGANCWWVLIPSLISFRCWKKICAAAAIANNNVETKTKKKTR 223

The following BLAST results are available for this feature:

BLAST of Csor.00g134260 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O48962	1.3e-74	61.61	Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Arabidopsis thalia...	[more]
Q9FTZ2	9.6e-65	57.82	Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Oryza sativa subsp...	[more]
Q9JJ46	1.1e-31	37.44	3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Rattus norvegicus OX=10116 ...	[more]
P70245	9.1e-31	36.24	3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Mus musculus OX=10090 GN=Eb...	[more]
Q60490	6.5e-29	37.56	3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cavia porcellus OX=10141 GN...	[more]

BLAST of Csor.00g134260 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6581969.1	7.97e-155	100.00	putative 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase, partial [Cucurbita a...	[more]
XP_022955667.1	3.25e-154	99.55	probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucurbita moschata]	[more]
XP_023526321.1	3.25e-154	99.10	probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucurbita pepo subsp...	[more]
XP_022980063.1	7.65e-153	98.20	probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucurbita maxima]	[more]
XP_038881035.1	1.54e-132	84.82	probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Benincasa hispida]	[more]

BLAST of Csor.00g134260 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GU98	1.57e-154	99.55	probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cucurbita moschata...	[more]
A0A6J1IY56	3.70e-153	98.20	probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cucurbita maxima O...	[more]
A0A6J1E0U0	1.60e-127	80.27	probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Momordica charanti...	[more]
A0A1S3BY31	2.18e-125	82.43	probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cucumis melo OX=36...	[more]
A0A0A0KVP4	1.79e-124	81.08	EXPERA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G007050 PE=3...	[more]

BLAST of Csor.00g134260 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G20050.1	9.5e-76	61.61	C-8,7 sterol isomerase	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF05241	EBP	coord: 95..203 e-value: 3.3E-21 score: 75.8
None	No IPR available	PANTHER	PTHR14207:SF7	BNAANNG14740D PROTEIN	coord: 6..215
IPR007905	Emopamil-binding protein	PANTHER	PTHR14207	STEROL ISOMERASE	coord: 6..215
IPR033118	EXPERA domain	PROSITE	PS51751	EXPERA	coord: 60..200 score: 16.254759

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g134260.m01	Csor.00g134260.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016125	sterol metabolic process
biological_process	GO:0016126	sterol biosynthetic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0000247	C-8 sterol isomerase activity
molecular_function	GO:0047750	cholestenol delta-isomerase activity
molecular_function	GO:0004769	steroid delta-isomerase activity