Csor.00g133540 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g133540
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description30S ribosomal protein S1 homolog B
LocationCsor_Chr14: 13319109 .. 13324225 (-)
RNA-Seq ExpressionCsor.00g133540
SyntenyCsor.00g133540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCAATCTTTGCTGCTACAACGCTCGGATCTCTATCTGCTCATTCATTTCTTTCGCTCTTAGCCTCCACTGATGCTTCTCACTCCCTTTCAAGCTCCTTCGTTTCAACCCATAAATCTCCCTCTAAACGCCCTTCCAACTTCGCCGCCCGAGTTTCCCTTTCCGGAAAACCGGAGCCCATTGCCGGAGTTTTGGAAAGTTCGCCTTCCTCGCCGGAGTCAGTTCGACGTGCTCGGGTGAGTACATTGTTCATCCTTTCTTTTAATTGAACAACCGGAGTTCTACGTTGTTTGTTTTTTAGTAAAATATAGCCGATTTTCTCTTTAAATGAATCACTCTCAAGTAACAAGTTTGCTGGATTCGAATTCGAATTCGAATTCTGGTTCCAAGTGCTGAAGGTTTTGAATGATTCATTAGAAGTGTATGTGTGAATCCTTCGGCAATACGGCAAGCTTTTGGCATTCAATTCCGTTAAGGACTCGATTTTGAGATTAGGTTTTGATTTTCACAGCCAGATATTCTGTTTCCATTCAACTATTTCACTTAGGGCATTGAATTGCAGCCTTATAGTTTTGTTGTTCAACCATGCAATATCAAGGGCTAAAGATGATGCCATGGGTTCAGAAGGATCATCATTTAGCCATCATGATTGGATGGTTGTTGGTGGGAGCAAAAAGTTTATGCTTAGCCTGAACTTGTATGAGTATTGTTAAACTCTTATATTCTAATACTTGGTTTCTGCAAAGAAGTAGAGATCTGCTGATTGGAAGACAGCGAGGGAATACCTTGATAGTGGATTTATCTTCAAAGGTAGGATTGAAGGTTCGAATGCTGGAGGTTTACTCGTCCGATTTTATTCTCTTGTGGGGTTTCTTCCATTCCCTCTATTGAGCCCTGCTCATTCTTGTAAAGGTACAAGTTCTCTTAATCCTTTGTGCTATATGCTATTAGACGAAGTCAGTAGTTTACCTAATAATGACTTGAATTGCTTGAGATATATGTGTTTCAAGTTTTTTTAACATGAATTTCTTGCAGAACCATACAAGAGTATCCAAGATATTGCAAAAAGCTTAATTGGTTCACTTATACCAGTGAAGGTAGTTGGTTGTCCTTAATTTTAATCGTTTATAGTTTTGAAAGCATCTCTTCTAGTTGGCTGAAGACTGGTGTATTTCTATTTGCTTCGCCACTCCCTAGTGTCCATCATGAGCACACTAGGTCATGTGTCAAACGTGTGTCCAACACACCAAAATAAAACTCATTTTTTATATTTGTTTCTTTAACTTTTCTTTTGAAACTCAGACATTTAGTACTTTCAATTAGCCACATGTTCACAGAATCTGAGACGGATATGAGGGGTATATCTTTGTTCAAGGGGAGGAGACTAAGGGCGCGATAGTCCTGACACCATGTACCCCACAATGTAATTTAGGCTTCTGCTTTGTCTAGGTTGTTGCCTTGGGGATGTCAAGAACAAACTCTCGTAGTCAATTGAGACGATTGAGAGTGGATGGATGAGTGTAGGTGTAAGTGGTACTTTGGTCAAGGAGAGAATGGTTAGGAGTGCAAACAATGTCTCACATCGACTAGGCAAGGGAGAGATCCATGCTTTATAAGTGAGAACAATTATTTCTATTGGTATGAGGCCTTTTAGGTGAATTAAAAGCAAAATCATGAGGTCTTAAACCAAAGTCGATAATACCATACCGTTGTAGAAATATGTGGAGGGTCGATTGTCCATAACAGAATTTTCATTTTTTTATGGAGTGGGGCACCAAAGACTTCCTTGTATTGAATCTTTCTTTCTATTTTCTTTTTCCGTGTTCTTCAGCTTTAGCTGGGACAGATGCTTCTTTGGCTCCTGTAACTTCTAGAGAAAATGATCACTCTCCCCTCCGGTTGAAATTTCTTGCTCACTGTCTCCACTTTGTTCCCTCTTATATTCTTCCTTGCCTCTGTCAAATATTTTCCTGAAGTTCCTCAAATCATTTCCCAAACTATGCACCAACATCTCTTTTGAAATTGAACTATGGGATGGTGCCCTTGCTTCTTCTGTTCTAAATCTAATGAATTAAAACTCAACTGATCCAATTTAGGTCCAAATTCTTTTATTTCAATCATAATTGGTTAATGAAGAAAGTAAAACACTTGGATCATGTGGCTACACCATATTACATGTATTCATAAAGTGAAACAGAGAAAGTTGTAATGTAATTGTTTATGAGGGAAATGAAGGAAAAAAATTAGGAGTCTATTCCATTTGGGCGAGATAGTTCTTTCATCATTCATATTCACAGTTGACCATCCCTGCTTTTGAAATATTTAGGTGAGAATACATTAAACCCTGTCTTTTCTTATTTTCTAGGTTATCCAAGCAGATGAGAAAAACAAGAATTTGATATTTTCAGAGAAGGAAGCTGCATGGTCAAAGTTTTCTGAGAAAGTTGGTGTGGGAGATGTCTATGAAGCTAGAGTTGGCTCTGTGGAGGATTATGGTGCCTTTGTACATTTACGTTTCTCTGATGGTGATATTTTGTGTTCTATTAAGCTTATGTACTGATATATTAAGAAATCAATTACCACTTATTTTCCCAAATTCTACAGGTCTCTATCATCTTACTGGGCTAGTACATATATCAGAAGTTTCATGGGATCTAGTTCAGGATGTAAGAGACATCTTAAGCGAGGGTGACGAAGTGAGGGTGAAAGTCATTGATGTTGATAGGCAAGTCTTGTCAGAGTTCTTTTTGTCACCAGCTAATTTGCACCTTCTAGGAAATTCTGTTATTTGAAGTATTTTTATCCACTATAAAACATATACAAGGCTTTCTCTTGATTACAAATAATTGTTATATAGTGTTATAATGAAGTGCAAATTATGAGCTCAATCATATTCAGCGCAAGCTTGTTAGCTAGCTAGTTACAGCCGTTATTGTTTGTAATCAGTGCTGTAACTAGAGATGGTATAGTTATTGTACCAAACTTACTTGTGAGATCCCACATCGGTTGGAGAGGGGAACGAAACATTTTTATAAGGGCGTGGAAATCTTTCTTTAGCAGACACGTATTAAAAACCTTGAGAGGAAGCCCGTAAGGAAAACCCAAAGAGTACAATATCTGCTAGCAGTGGGCTTAGGCTGTTACAAATGGTAGTAGAGCCAACACCGGACGGTGTGCTAGCGAGGACGCTGAGCCCAGAAGGGGGTGGACACTAGGCCCCGTAGGGGGGCGGATTGTGTGATCCCATATTGGTTGGAGAGGGGAACGAAATATTATTTATAAGGGTGTGGAAACTTCTCCCTAGTAGACGCATATTAAAAACCTTGAGGGGAAGCTCGAAAGGGAAAACCCAAAGAGAACAATATCTGCAAGTGGTGGGCTTGAATTGTTACATTATCGATGAAACATGAAATATAGACTCGTTCTTTGGTGGATGTAGACCAATTATTGGGTCGAACCACTTAAAAATCTTGTGAGTTCCTCTGCTGTTTCTTTCCTTCTTAATCTTTGTAATTGTTCTGATTCTGGTTAAAACCCAGATTAGCCATAGGCGATAGATGATTTTGTTATTTTTGTAGAAGGTTAAAAAATGGAAAACGAGGAAAATGTGATAAGACTTGCTAAATTTATATCATCTCAAGCTTTTAGGCCATTGCCTATTGCCATGCATTGTCAGCTGTCTTATTGATTTTATGCAGGGACAAGTCGAGGATCACATTATCCATTAAACAACTCGAGGAAGATCCACTTTTGGAAACATTGGACAAAGTAATACCGCAGGTTAGTGAAGCATTGATGTACGCATTTAGTCAGCCTTTTCAACTACTTTCATCTCATAACTGAGCTTTAACTACATGAACATCATAATTTATTATAACAGGATGATTCTGCTGAACCTGATTCATTCGGACCTAAAAGTGACAGCGAAATCATACCCCTTCCTGGACTTGATACAATATTTGAAGAGCTACTGCAAGAAGAAGGGTATGTTTCTCTAATCTTCAAAGCTATGGAGAGCACTGTCTAAAAAGATTACTGGTAACAACATGGACATCAACAGTACTAATAAATATGCCTTGGCTTGTTCTGAATTTATGAGTTTAACTTTTGTGTTAGACAGACAATGTGATGTACATACAAATATTTATATACATTCATACACATACGTTTAGGGTTCAAATATGAGATGAATTGGCTGACACTGAACAAACTAAAGAGGGCTTGTCTGGGACTTCGTGCATTTTTTTCACTTCATTGATAAACTTTGTTAGATTTTTAGTCATTGGTAGGGATCATATTATCAGCATGTGAGTTCTCAAGTTATATTAACCTTGAGTCAGCAGTATGATCTTAATACGACTGGTTTGAATATCAGTATAGAAGATGTTCATATCAACCGACAAGGATTTGAGAAACGGGTGGTTTCACAAGACCTACAGCTATGGCTATCAAATGTAAGATGGTTTGTTACTAAGACTATTATGTGGGGTTTAGTCATTTGCTTCAATTTCTCAATTGTCATCTTACTGTTCTTGTAGGCACCTCCTGTTGAAAAGAAGTTCACTCTCCTTGCTCGTGCCGGGAGGCAGGTAGTGTTTCTTCTTCTCACTCACACACAAGCATGAGCACATGCATACAGATACTAACTATAACGGCCCAAGCCCACTACTAGCAGATATTGTCTCTTTGGGCTTTCTCTTTCGAGTTTCTCTCAAGGTTTTTAAAACGCATTTGTTAAGGAGAGGTTTCCACGCCCTTATAAAGAATGTTTCATTCCCCTCTCCAACCGATGTGGGATCTCACAATCCACCCCTTTGGGGACCCAGCGTCCTTGCTGCGAGTGTGTGGTTCTGATACCATTTGTAATGGCCCAAGGCCACTGCTAGCATATATTGTCCTCTTTGGGCTTTCCCTCAAAGTTTTTAAAACGCGTCTACTAGGGGAGGTTTCCACACCCTTATAAAGAATGTTTTGTTCTCCCTTCCAACTAACGTAGGATCTCACACTTATCACTAATTGTTTGTAAATTTAGGTTCAAGAAATACAGCTGACAACATCACTCGATCAGGAAGGTATTAAAAGGGCATTGCAGCGAGTATTGGAACGTGTCCCATGA

mRNA sequence

ATGCCAATCTTTGCTGCTACAACGCTCGGATCTCTATCTGCTCATTCATTTCTTTCGCTCTTAGCCTCCACTGATGCTTCTCACTCCCTTTCAAGCTCCTTCGTTTCAACCCATAAATCTCCCTCTAAACGCCCTTCCAACTTCGCCGCCCGAGTTTCCCTTTCCGGAAAACCGGAGCCCATTGCCGGAGTTTTGGAAAGTTCGCCTTCCTCGCCGGAGTCAGTTCGACGTGCTCGGAGATCTGCTGATTGGAAGACAGCGAGGGAATACCTTGATAGTGGATTTATCTTCAAAGGTAGGATTGAAGGTTCGAATGCTGGAGGTTTACTCGTCCGATTTTATTCTCTTGTGGGGTTTCTTCCATTCCCTCTATTGAGCCCTGCTCATTCTTGTAAAGAACCATACAAGAGTATCCAAGATATTGCAAAAAGCTTAATTGGTTCACTTATACCAGTGAAGGTTATCCAAGCAGATGAGAAAAACAAGAATTTGATATTTTCAGAGAAGGAAGCTGCATGGTCAAAGTTTTCTGAGAAAGTTGGTGTGGGAGATGTCTATGAAGCTAGAGTTGGCTCTGTGGAGGATTATGGTGCCTTTGTACATTTACGTTTCTCTGATGGTCTCTATCATCTTACTGGGCTAGTACATATATCAGAAGTTTCATGGGATCTAGTTCAGGATGTAAGAGACATCTTAAGCGAGGGTGACGAAGTGAGGGTGAAAGTCATTGATGTTGATAGGGACAAGTCGAGGATCACATTATCCATTAAACAACTCGAGGAAGATCCACTTTTGGAAACATTGGACAAAGTAATACCGCAGGATGATTCTGCTGAACCTGATTCATTCGGACCTAAAAGTGACAGCGAAATCATACCCCTTCCTGGACTTGATACAATATTTGAAGAGCTACTGCAAGAAGAAGGTATAGAAGATGTTCATATCAACCGACAAGGATTTGAGAAACGGGTGGTTTCACAAGACCTACAGCTATGGCTATCAAATGCACCTCCTGTTGAAAAGAAGTTCACTCTCCTTGCTCGTGCCGGGAGGCAGGTTCAAGAAATACAGCTGACAACATCACTCGATCAGGAAGGTATTAAAAGGGCATTGCAGCGAGTATTGGAACGTGTCCCATGA

Coding sequence (CDS)

ATGCCAATCTTTGCTGCTACAACGCTCGGATCTCTATCTGCTCATTCATTTCTTTCGCTCTTAGCCTCCACTGATGCTTCTCACTCCCTTTCAAGCTCCTTCGTTTCAACCCATAAATCTCCCTCTAAACGCCCTTCCAACTTCGCCGCCCGAGTTTCCCTTTCCGGAAAACCGGAGCCCATTGCCGGAGTTTTGGAAAGTTCGCCTTCCTCGCCGGAGTCAGTTCGACGTGCTCGGAGATCTGCTGATTGGAAGACAGCGAGGGAATACCTTGATAGTGGATTTATCTTCAAAGGTAGGATTGAAGGTTCGAATGCTGGAGGTTTACTCGTCCGATTTTATTCTCTTGTGGGGTTTCTTCCATTCCCTCTATTGAGCCCTGCTCATTCTTGTAAAGAACCATACAAGAGTATCCAAGATATTGCAAAAAGCTTAATTGGTTCACTTATACCAGTGAAGGTTATCCAAGCAGATGAGAAAAACAAGAATTTGATATTTTCAGAGAAGGAAGCTGCATGGTCAAAGTTTTCTGAGAAAGTTGGTGTGGGAGATGTCTATGAAGCTAGAGTTGGCTCTGTGGAGGATTATGGTGCCTTTGTACATTTACGTTTCTCTGATGGTCTCTATCATCTTACTGGGCTAGTACATATATCAGAAGTTTCATGGGATCTAGTTCAGGATGTAAGAGACATCTTAAGCGAGGGTGACGAAGTGAGGGTGAAAGTCATTGATGTTGATAGGGACAAGTCGAGGATCACATTATCCATTAAACAACTCGAGGAAGATCCACTTTTGGAAACATTGGACAAAGTAATACCGCAGGATGATTCTGCTGAACCTGATTCATTCGGACCTAAAAGTGACAGCGAAATCATACCCCTTCCTGGACTTGATACAATATTTGAAGAGCTACTGCAAGAAGAAGGTATAGAAGATGTTCATATCAACCGACAAGGATTTGAGAAACGGGTGGTTTCACAAGACCTACAGCTATGGCTATCAAATGCACCTCCTGTTGAAAAGAAGTTCACTCTCCTTGCTCGTGCCGGGAGGCAGGTTCAAGAAATACAGCTGACAACATCACTCGATCAGGAAGGTATTAAAAGGGCATTGCAGCGAGTATTGGAACGTGTCCCATGA

Protein sequence

MPIFAATTLGSLSAHSFLSLLASTDASHSLSSSFVSTHKSPSKRPSNFAARVSLSGKPEPIAGVLESSPSSPESVRRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLVGFLPFPLLSPAHSCKEPYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFSEKVGVGDVYEARVGSVEDYGAFVHLRFSDGLYHLTGLVHISEVSWDLVQDVRDILSEGDEVRVKVIDVDRDKSRITLSIKQLEEDPLLETLDKVIPQDDSAEPDSFGPKSDSEIIPLPGLDTIFEELLQEEGIEDVHINRQGFEKRVVSQDLQLWLSNAPPVEKKFTLLARAGRQVQEIQLTTSLDQEGIKRALQRVLERVP
Homology
BLAST of Csor.00g133540 vs. ExPASy Swiss-Prot
Match: P29344 (30S ribosomal protein S1, chloroplastic OS=Spinacia oleracea OX=3562 GN=RPS1 PE=1 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 1.7e-21
Identity = 62/203 (30.54%), Postives = 108/203 (53.20%), Query Frame = 0

Query: 61  IAGVLESSPSSPESVRRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLVGFL 120
           I G  E+  S   S+R+ +    W+  R+      + KG+I G+N GG++     L GF+
Sbjct: 151 IIGENEADDSLILSLRQIQYELAWERCRQLQAEDVVVKGKIVGANKGGVVALVEGLRGFV 210

Query: 121 PFPLLSPAHSCKEPYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFSEKV 180
           PF  +S   S +E           L+   IP+K ++ DE+   L+ S ++ A +    ++
Sbjct: 211 PFSQISSKSSAEE-----------LLEKEIPLKFVEVDEEQSRLVMSNRK-AMADSQAQL 270

Query: 181 GVGDVYEARVGSVEDYGAFVHLRFSDGLYHLTGLVHISEVSWDLVQDVRDILSEGDEVRV 240
           G+G V    V S++ YGAF+ +        + GL+H+S++S D V D+  +L  GD ++V
Sbjct: 271 GIGSVVTGTVQSLKPYGAFIDIG------GINGLLHVSQISHDRVSDIATVLQPGDTLKV 330

Query: 241 KVIDVDRDKSRITLSIKQLEEDP 264
            ++  DR++ R++LS K+LE  P
Sbjct: 331 MILSHDRERGRVSLSTKKLEPTP 335

BLAST of Csor.00g133540 vs. ExPASy Swiss-Prot
Match: P46228 (30S ribosomal protein S1 OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) OX=269084 GN=rpsA PE=1 SV=4)

HSP 1 Score: 100.1 bits (248), Expect = 5.5e-20
Identity = 57/190 (30.00%), Postives = 97/190 (51.05%), Query Frame = 0

Query: 74  SVRRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLVGFLPFPLLSPAHSCKE 133
           S+RR      W+  R+        +  +  +N GG LVR   L GF+P            
Sbjct: 99  SIRRIEYMRAWERVRQLQTEDATVRSEVFATNRGGALVRIEGLRGFIP-----------G 158

Query: 134 PYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFSEKVGVGDVYEARVGSV 193
            + S +   + L+G  +P+K ++ DE    L+ S + A   +   ++ VG+V    V  +
Sbjct: 159 SHISTRKAKEDLVGEELPLKFLEVDEDRNRLVLSHRRALVERKMNRLEVGEVVVGAVRGI 218

Query: 194 EDYGAFVHLRFSDGLYHLTGLVHISEVSWDLVQDVRDILSEGDEVRVKVIDVDRDKSRIT 253
           + YGAF+ +        ++GL+HISE+S D ++    + +  DEV+V +ID+D ++ RI+
Sbjct: 219 KPYGAFIDIG------GVSGLLHISEISHDHIETPHSVFNVNDEVKVMIIDLDAERGRIS 271

Query: 254 LSIKQLEEDP 264
           LS KQLE +P
Sbjct: 279 LSTKQLEPEP 271

BLAST of Csor.00g133540 vs. ExPASy Swiss-Prot
Match: Q93VC7 (30S ribosomal protein S1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RPS1 PE=1 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 2.1e-19
Identity = 73/248 (29.44%), Postives = 125/248 (50.40%), Query Frame = 0

Query: 24  TDASHSL------SSSFVSTHKSPSKRPSNFAARVSLSGKPEP--IAGVLESSPSSPESV 83
           TDA+ +L      SS+++S  ++   R  +      + G  E   I G  ES  S   S+
Sbjct: 109 TDANGALVDISAKSSAYLSVEQACIHRIKHVEEAGIVPGMVEEFVIIGENESDDSLLLSL 168

Query: 84  RRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLVGFLPFPLLSPAHSCKEPY 143
           R  +    W+  R+      I K ++ G+N GGL+     L GF+PF  +S   + +E  
Sbjct: 169 RNIQYELAWERCRQLQAEDVIVKAKVIGANKGGLVALVEGLRGFVPFSQISSKAAAEE-- 228

Query: 144 KSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFSEKVGVGDVYEARVGSVED 203
                    L+   IP+K ++ DE+   L+ S ++A  +    ++G+G V    V S++ 
Sbjct: 229 ---------LLEKEIPLKFVEVDEEQTKLVLSNRKAV-ADSQAQLGIGSVVLGVVQSLKP 288

Query: 204 YGAFVHLRFSDGLYHLTGLVHISEVSWDLVQDVRDILSEGDEVRVKVIDVDRDKSRITLS 263
           YGAF+ +        + GL+H+S++S D V D+  +L  GD ++V ++  DRD+ R++LS
Sbjct: 289 YGAFIDIG------GINGLLHVSQISHDRVSDIATVLQPGDTLKVMILSHDRDRGRVSLS 338

BLAST of Csor.00g133540 vs. ExPASy Swiss-Prot
Match: Q9JZ44 (30S ribosomal protein S1 OS=Neisseria meningitidis serogroup B (strain MC58) OX=122586 GN=rpsA PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 2.7e-19
Identity = 65/197 (32.99%), Postives = 101/197 (51.27%), Query Frame = 0

Query: 74  SVRRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLVGFLPFPLLSPAHSCKE 133
           S  +A+R+ADW    E +++G I  G I G   GGL V   S+  FLP  L+        
Sbjct: 86  SREKAKRAADWIALEEAMENGDILSGIINGKVKGGLTVMISSIRAFLPGSLVD------- 145

Query: 134 PYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFSEK-------VGVGDVY 193
             + ++D      G  I  KVI+ D+K  N++ S +    +   E+       +  G V 
Sbjct: 146 -VRPVKD-TSHFEGKEIEFKVIKLDKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVI 205

Query: 194 EARVGSVEDYGAFVHLRFSDGLYHLTGLVHISEVSWDLVQDVRDILSEGDEVRVKVIDVD 253
           +  V ++ DYGAFV L   D      GL+HI++++W  V+   ++L  G EV  KV+  D
Sbjct: 206 KGIVKNITDYGAFVDLGGID------GLLHITDLAWRRVKHPSEVLEVGQEVEAKVLKFD 265

Query: 254 RDKSRITLSIKQLEEDP 264
           ++K R++L +KQL EDP
Sbjct: 266 QEKQRVSLGMKQLGEDP 267

BLAST of Csor.00g133540 vs. ExPASy Swiss-Prot
Match: P73530 (30S ribosomal protein S1 homolog A OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=rps1A PE=3 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 8.0e-19
Identity = 58/190 (30.53%), Postives = 95/190 (50.00%), Query Frame = 0

Query: 74  SVRRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLVGFLPFPLLSPAHSCKE 133
           S+RR      W+  R+        +  +  +N GG LVR   L GF+P   +S A   KE
Sbjct: 98  SIRRIEYMRAWERVRQLQAEDATVRSNVFATNRGGALVRIEGLRGFIPGSHIS-AREAKE 157

Query: 134 PYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFSEKVGVGDVYEARVGSV 193
                      L+G  +P+K ++ DE+   L+ S + A   +    + V  V    V  +
Sbjct: 158 ----------DLVGEDLPLKFLEVDEERNRLVLSHRRALVERKMNGLEVAQVVVGSVRGI 217

Query: 194 EDYGAFVHLRFSDGLYHLTGLVHISEVSWDLVQDVRDILSEGDEVRVKVIDVDRDKSRIT 253
           + YGAF+ +        ++GL+HISE+S D +     + +  DE++V +ID+D ++ RI+
Sbjct: 218 KPYGAFIDIG------GVSGLLHISEISHDHIDTPHSVFNVNDEIKVMIIDLDAERGRIS 270

Query: 254 LSIKQLEEDP 264
           LS KQLE +P
Sbjct: 278 LSTKQLEPEP 270

BLAST of Csor.00g133540 vs. NCBI nr
Match: KAG6582039.1 (30S ribosomal protein S1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 726 bits (1874), Expect = 1.45e-263
Identity = 379/379 (100.00%), Postives = 379/379 (100.00%), Query Frame = 0

Query: 1   MPIFAATTLGSLSAHSFLSLLASTDASHSLSSSFVSTHKSPSKRPSNFAARVSLSGKPEP 60
           MPIFAATTLGSLSAHSFLSLLASTDASHSLSSSFVSTHKSPSKRPSNFAARVSLSGKPEP
Sbjct: 1   MPIFAATTLGSLSAHSFLSLLASTDASHSLSSSFVSTHKSPSKRPSNFAARVSLSGKPEP 60

Query: 61  IAGVLESSPSSPESVRRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLVGFL 120
           IAGVLESSPSSPESVRRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLVGFL
Sbjct: 61  IAGVLESSPSSPESVRRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLVGFL 120

Query: 121 PFPLLSPAHSCKEPYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFSEKV 180
           PFPLLSPAHSCKEPYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFSEKV
Sbjct: 121 PFPLLSPAHSCKEPYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFSEKV 180

Query: 181 GVGDVYEARVGSVEDYGAFVHLRFSDGLYHLTGLVHISEVSWDLVQDVRDILSEGDEVRV 240
           GVGDVYEARVGSVEDYGAFVHLRFSDGLYHLTGLVHISEVSWDLVQDVRDILSEGDEVRV
Sbjct: 181 GVGDVYEARVGSVEDYGAFVHLRFSDGLYHLTGLVHISEVSWDLVQDVRDILSEGDEVRV 240

Query: 241 KVIDVDRDKSRITLSIKQLEEDPLLETLDKVIPQDDSAEPDSFGPKSDSEIIPLPGLDTI 300
           KVIDVDRDKSRITLSIKQLEEDPLLETLDKVIPQDDSAEPDSFGPKSDSEIIPLPGLDTI
Sbjct: 241 KVIDVDRDKSRITLSIKQLEEDPLLETLDKVIPQDDSAEPDSFGPKSDSEIIPLPGLDTI 300

Query: 301 FEELLQEEGIEDVHINRQGFEKRVVSQDLQLWLSNAPPVEKKFTLLARAGRQVQEIQLTT 360
           FEELLQEEGIEDVHINRQGFEKRVVSQDLQLWLSNAPPVEKKFTLLARAGRQVQEIQLTT
Sbjct: 301 FEELLQEEGIEDVHINRQGFEKRVVSQDLQLWLSNAPPVEKKFTLLARAGRQVQEIQLTT 360

Query: 361 SLDQEGIKRALQRVLERVP 379
           SLDQEGIKRALQRVLERVP
Sbjct: 361 SLDQEGIKRALQRVLERVP 379

BLAST of Csor.00g133540 vs. NCBI nr
Match: XP_022979820.1 (uncharacterized protein LOC111479406 [Cucurbita maxima])

HSP 1 Score: 720 bits (1858), Expect = 3.98e-261
Identity = 375/379 (98.94%), Postives = 377/379 (99.47%), Query Frame = 0

Query: 1   MPIFAATTLGSLSAHSFLSLLASTDASHSLSSSFVSTHKSPSKRPSNFAARVSLSGKPEP 60
           MPIFAATTLGSLSAHSFLSLLASTDASHSLSSSFV THKSPSKRPSNFAARVSLSGKPEP
Sbjct: 1   MPIFAATTLGSLSAHSFLSLLASTDASHSLSSSFVLTHKSPSKRPSNFAARVSLSGKPEP 60

Query: 61  IAGVLESSPSSPESVRRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLVGFL 120
           IAGVLESSPSSPESVRRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLVGFL
Sbjct: 61  IAGVLESSPSSPESVRRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLVGFL 120

Query: 121 PFPLLSPAHSCKEPYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFSEKV 180
           PFPLLSPAHSCKEPYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFSE+V
Sbjct: 121 PFPLLSPAHSCKEPYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFSEQV 180

Query: 181 GVGDVYEARVGSVEDYGAFVHLRFSDGLYHLTGLVHISEVSWDLVQDVRDILSEGDEVRV 240
           GVGDVYEARVGSVEDYGAFVHLRFSDG YHLTGLVHISEVSWDLVQDVRDILSEGDEVRV
Sbjct: 181 GVGDVYEARVGSVEDYGAFVHLRFSDGFYHLTGLVHISEVSWDLVQDVRDILSEGDEVRV 240

Query: 241 KVIDVDRDKSRITLSIKQLEEDPLLETLDKVIPQDDSAEPDSFGPKSDSEIIPLPGLDTI 300
           KV+DVDRDKSRITLSIKQLEEDPLLETLDKVIPQDDSAEPDSFGPKSDSEIIPLPGLDTI
Sbjct: 241 KVLDVDRDKSRITLSIKQLEEDPLLETLDKVIPQDDSAEPDSFGPKSDSEIIPLPGLDTI 300

Query: 301 FEELLQEEGIEDVHINRQGFEKRVVSQDLQLWLSNAPPVEKKFTLLARAGRQVQEIQLTT 360
           FEELLQEEGIEDVHINRQGFEKRVVSQDLQLWLSNAPPVEKKFTLLARAGRQVQEIQLTT
Sbjct: 301 FEELLQEEGIEDVHINRQGFEKRVVSQDLQLWLSNAPPVEKKFTLLARAGRQVQEIQLTT 360

Query: 361 SLDQEGIKRALQRVLERVP 379
           SLDQEGIKRALQRVLERVP
Sbjct: 361 SLDQEGIKRALQRVLERVP 379

BLAST of Csor.00g133540 vs. NCBI nr
Match: KAG7018465.1 (30S ribosomal protein S1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 714 bits (1844), Expect = 5.42e-259
Identity = 373/379 (98.42%), Postives = 376/379 (99.21%), Query Frame = 0

Query: 1   MPIFAATTLGSLSAHSFLSLLASTDASHSLSSSFVSTHKSPSKRPSNFAARVSLSGKPEP 60
           MPIFAATTLGSLSAHSFLSL AS DAS+SLS+SF+ THKSPSKRPSNFAARVSLSGKPEP
Sbjct: 1   MPIFAATTLGSLSAHSFLSLSASADASNSLSTSFLLTHKSPSKRPSNFAARVSLSGKPEP 60

Query: 61  IAGVLESSPSSPESVRRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLVGFL 120
           IAGVLESSPSSPESVRRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLVGFL
Sbjct: 61  IAGVLESSPSSPESVRRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLVGFL 120

Query: 121 PFPLLSPAHSCKEPYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFSEKV 180
           PFPLLSPAHSCKEPYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFSEKV
Sbjct: 121 PFPLLSPAHSCKEPYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFSEKV 180

Query: 181 GVGDVYEARVGSVEDYGAFVHLRFSDGLYHLTGLVHISEVSWDLVQDVRDILSEGDEVRV 240
           GVGDVYEARVGSVEDYGAFVHLRFSDGLYHLTGLVHISEVSWDLVQDVRDILSEGDEVRV
Sbjct: 181 GVGDVYEARVGSVEDYGAFVHLRFSDGLYHLTGLVHISEVSWDLVQDVRDILSEGDEVRV 240

Query: 241 KVIDVDRDKSRITLSIKQLEEDPLLETLDKVIPQDDSAEPDSFGPKSDSEIIPLPGLDTI 300
           KVIDVDRDKSRITLSIKQLEEDPLLETLDKVIPQDDSAEPDSFGPKSDSEIIPLPGLDTI
Sbjct: 241 KVIDVDRDKSRITLSIKQLEEDPLLETLDKVIPQDDSAEPDSFGPKSDSEIIPLPGLDTI 300

Query: 301 FEELLQEEGIEDVHINRQGFEKRVVSQDLQLWLSNAPPVEKKFTLLARAGRQVQEIQLTT 360
           FEELLQEEGIEDVHINRQGFEKRVVSQDLQLWLSNAPPVEKKFTLLARAGRQVQEIQLTT
Sbjct: 301 FEELLQEEGIEDVHINRQGFEKRVVSQDLQLWLSNAPPVEKKFTLLARAGRQVQEIQLTT 360

Query: 361 SLDQEGIKRALQRVLERVP 379
           SLDQEGIKRALQRVLERVP
Sbjct: 361 SLDQEGIKRALQRVLERVP 379

BLAST of Csor.00g133540 vs. NCBI nr
Match: XP_022955545.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111457527 [Cucurbita moschata])

HSP 1 Score: 713 bits (1841), Expect = 8.68e-258
Identity = 372/379 (98.15%), Postives = 376/379 (99.21%), Query Frame = 0

Query: 1   MPIFAATTLGSLSAHSFLSLLASTDASHSLSSSFVSTHKSPSKRPSNFAARVSLSGKPEP 60
           MPIFAATTLGSLSAHSFLSL AS DAS+SLS+SF+ THKSPSKRPSNFAARVSLSGKPEP
Sbjct: 47  MPIFAATTLGSLSAHSFLSLSASADASNSLSTSFLLTHKSPSKRPSNFAARVSLSGKPEP 106

Query: 61  IAGVLESSPSSPESVRRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLVGFL 120
           IAGVLESSPSSPESVRRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLVGFL
Sbjct: 107 IAGVLESSPSSPESVRRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLVGFL 166

Query: 121 PFPLLSPAHSCKEPYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFSEKV 180
           PFPLLSPAHSCKEPYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFSEKV
Sbjct: 167 PFPLLSPAHSCKEPYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFSEKV 226

Query: 181 GVGDVYEARVGSVEDYGAFVHLRFSDGLYHLTGLVHISEVSWDLVQDVRDILSEGDEVRV 240
           GVGDVYEARVGS+EDYGAFVHLRFSDGLYHLTGLVHISEVSWDLVQDVRDILSEGDEVRV
Sbjct: 227 GVGDVYEARVGSLEDYGAFVHLRFSDGLYHLTGLVHISEVSWDLVQDVRDILSEGDEVRV 286

Query: 241 KVIDVDRDKSRITLSIKQLEEDPLLETLDKVIPQDDSAEPDSFGPKSDSEIIPLPGLDTI 300
           KVIDVDRDKSRITLSIKQLEEDPLLETLDKVIPQDDSAEPDSFGPKSDSEIIPLPGLDTI
Sbjct: 287 KVIDVDRDKSRITLSIKQLEEDPLLETLDKVIPQDDSAEPDSFGPKSDSEIIPLPGLDTI 346

Query: 301 FEELLQEEGIEDVHINRQGFEKRVVSQDLQLWLSNAPPVEKKFTLLARAGRQVQEIQLTT 360
           FEELLQEEGIEDVHINRQGFEKRVVSQDLQLWLSNAPPVEKKFTLLARAGRQVQEIQLTT
Sbjct: 347 FEELLQEEGIEDVHINRQGFEKRVVSQDLQLWLSNAPPVEKKFTLLARAGRQVQEIQLTT 406

Query: 361 SLDQEGIKRALQRVLERVP 379
           SLDQEGIKRALQRVLERVP
Sbjct: 407 SLDQEGIKRALQRVLERVP 425

BLAST of Csor.00g133540 vs. NCBI nr
Match: XP_023526022.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111789621 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 712 bits (1837), Expect = 3.53e-257
Identity = 371/379 (97.89%), Postives = 376/379 (99.21%), Query Frame = 0

Query: 1   MPIFAATTLGSLSAHSFLSLLASTDASHSLSSSFVSTHKSPSKRPSNFAARVSLSGKPEP 60
           MPIFAATTLGSLSAHSFL+L ASTDAS+SLS+SF  THKSPSKRPSNFAARVSLSGKP+P
Sbjct: 47  MPIFAATTLGSLSAHSFLALSASTDASNSLSTSFFLTHKSPSKRPSNFAARVSLSGKPDP 106

Query: 61  IAGVLESSPSSPESVRRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLVGFL 120
           IAGVLESSPSSPESVRRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLVGFL
Sbjct: 107 IAGVLESSPSSPESVRRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLVGFL 166

Query: 121 PFPLLSPAHSCKEPYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFSEKV 180
           PFPLLSPAHSCKEPYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFSE+V
Sbjct: 167 PFPLLSPAHSCKEPYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFSEQV 226

Query: 181 GVGDVYEARVGSVEDYGAFVHLRFSDGLYHLTGLVHISEVSWDLVQDVRDILSEGDEVRV 240
           GVGDVYEARVGSVEDYGAFVHLRFSDGLYHLTGLVHISEVSWDLVQDVRDILSEGDEVRV
Sbjct: 227 GVGDVYEARVGSVEDYGAFVHLRFSDGLYHLTGLVHISEVSWDLVQDVRDILSEGDEVRV 286

Query: 241 KVIDVDRDKSRITLSIKQLEEDPLLETLDKVIPQDDSAEPDSFGPKSDSEIIPLPGLDTI 300
           KVIDVDRDKSRITLSIKQLEEDPLLETLDKVIPQDDSAEPDSFGPKSDSEIIPLPGLDTI
Sbjct: 287 KVIDVDRDKSRITLSIKQLEEDPLLETLDKVIPQDDSAEPDSFGPKSDSEIIPLPGLDTI 346

Query: 301 FEELLQEEGIEDVHINRQGFEKRVVSQDLQLWLSNAPPVEKKFTLLARAGRQVQEIQLTT 360
           FEELLQEEGIEDVHINRQGFEKRVVSQDLQLWLSNAPPVEKKFTLLARAGRQVQEIQLTT
Sbjct: 347 FEELLQEEGIEDVHINRQGFEKRVVSQDLQLWLSNAPPVEKKFTLLARAGRQVQEIQLTT 406

Query: 361 SLDQEGIKRALQRVLERVP 379
           SLDQEGIKRALQRVLERVP
Sbjct: 407 SLDQEGIKRALQRVLERVP 425

BLAST of Csor.00g133540 vs. ExPASy TrEMBL
Match: A0A6J1IUF3 (uncharacterized protein LOC111479406 OS=Cucurbita maxima OX=3661 GN=LOC111479406 PE=4 SV=1)

HSP 1 Score: 720 bits (1858), Expect = 1.93e-261
Identity = 375/379 (98.94%), Postives = 377/379 (99.47%), Query Frame = 0

Query: 1   MPIFAATTLGSLSAHSFLSLLASTDASHSLSSSFVSTHKSPSKRPSNFAARVSLSGKPEP 60
           MPIFAATTLGSLSAHSFLSLLASTDASHSLSSSFV THKSPSKRPSNFAARVSLSGKPEP
Sbjct: 1   MPIFAATTLGSLSAHSFLSLLASTDASHSLSSSFVLTHKSPSKRPSNFAARVSLSGKPEP 60

Query: 61  IAGVLESSPSSPESVRRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLVGFL 120
           IAGVLESSPSSPESVRRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLVGFL
Sbjct: 61  IAGVLESSPSSPESVRRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLVGFL 120

Query: 121 PFPLLSPAHSCKEPYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFSEKV 180
           PFPLLSPAHSCKEPYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFSE+V
Sbjct: 121 PFPLLSPAHSCKEPYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFSEQV 180

Query: 181 GVGDVYEARVGSVEDYGAFVHLRFSDGLYHLTGLVHISEVSWDLVQDVRDILSEGDEVRV 240
           GVGDVYEARVGSVEDYGAFVHLRFSDG YHLTGLVHISEVSWDLVQDVRDILSEGDEVRV
Sbjct: 181 GVGDVYEARVGSVEDYGAFVHLRFSDGFYHLTGLVHISEVSWDLVQDVRDILSEGDEVRV 240

Query: 241 KVIDVDRDKSRITLSIKQLEEDPLLETLDKVIPQDDSAEPDSFGPKSDSEIIPLPGLDTI 300
           KV+DVDRDKSRITLSIKQLEEDPLLETLDKVIPQDDSAEPDSFGPKSDSEIIPLPGLDTI
Sbjct: 241 KVLDVDRDKSRITLSIKQLEEDPLLETLDKVIPQDDSAEPDSFGPKSDSEIIPLPGLDTI 300

Query: 301 FEELLQEEGIEDVHINRQGFEKRVVSQDLQLWLSNAPPVEKKFTLLARAGRQVQEIQLTT 360
           FEELLQEEGIEDVHINRQGFEKRVVSQDLQLWLSNAPPVEKKFTLLARAGRQVQEIQLTT
Sbjct: 301 FEELLQEEGIEDVHINRQGFEKRVVSQDLQLWLSNAPPVEKKFTLLARAGRQVQEIQLTT 360

Query: 361 SLDQEGIKRALQRVLERVP 379
           SLDQEGIKRALQRVLERVP
Sbjct: 361 SLDQEGIKRALQRVLERVP 379

BLAST of Csor.00g133540 vs. ExPASy TrEMBL
Match: A0A6J1GVD7 (LOW QUALITY PROTEIN: uncharacterized protein LOC111457527 OS=Cucurbita moschata OX=3662 GN=LOC111457527 PE=4 SV=1)

HSP 1 Score: 713 bits (1841), Expect = 4.20e-258
Identity = 372/379 (98.15%), Postives = 376/379 (99.21%), Query Frame = 0

Query: 1   MPIFAATTLGSLSAHSFLSLLASTDASHSLSSSFVSTHKSPSKRPSNFAARVSLSGKPEP 60
           MPIFAATTLGSLSAHSFLSL AS DAS+SLS+SF+ THKSPSKRPSNFAARVSLSGKPEP
Sbjct: 47  MPIFAATTLGSLSAHSFLSLSASADASNSLSTSFLLTHKSPSKRPSNFAARVSLSGKPEP 106

Query: 61  IAGVLESSPSSPESVRRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLVGFL 120
           IAGVLESSPSSPESVRRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLVGFL
Sbjct: 107 IAGVLESSPSSPESVRRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLVGFL 166

Query: 121 PFPLLSPAHSCKEPYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFSEKV 180
           PFPLLSPAHSCKEPYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFSEKV
Sbjct: 167 PFPLLSPAHSCKEPYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFSEKV 226

Query: 181 GVGDVYEARVGSVEDYGAFVHLRFSDGLYHLTGLVHISEVSWDLVQDVRDILSEGDEVRV 240
           GVGDVYEARVGS+EDYGAFVHLRFSDGLYHLTGLVHISEVSWDLVQDVRDILSEGDEVRV
Sbjct: 227 GVGDVYEARVGSLEDYGAFVHLRFSDGLYHLTGLVHISEVSWDLVQDVRDILSEGDEVRV 286

Query: 241 KVIDVDRDKSRITLSIKQLEEDPLLETLDKVIPQDDSAEPDSFGPKSDSEIIPLPGLDTI 300
           KVIDVDRDKSRITLSIKQLEEDPLLETLDKVIPQDDSAEPDSFGPKSDSEIIPLPGLDTI
Sbjct: 287 KVIDVDRDKSRITLSIKQLEEDPLLETLDKVIPQDDSAEPDSFGPKSDSEIIPLPGLDTI 346

Query: 301 FEELLQEEGIEDVHINRQGFEKRVVSQDLQLWLSNAPPVEKKFTLLARAGRQVQEIQLTT 360
           FEELLQEEGIEDVHINRQGFEKRVVSQDLQLWLSNAPPVEKKFTLLARAGRQVQEIQLTT
Sbjct: 347 FEELLQEEGIEDVHINRQGFEKRVVSQDLQLWLSNAPPVEKKFTLLARAGRQVQEIQLTT 406

Query: 361 SLDQEGIKRALQRVLERVP 379
           SLDQEGIKRALQRVLERVP
Sbjct: 407 SLDQEGIKRALQRVLERVP 425

BLAST of Csor.00g133540 vs. ExPASy TrEMBL
Match: A0A1S3BXL4 (30S ribosomal protein S1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494553 PE=4 SV=1)

HSP 1 Score: 612 bits (1579), Expect = 2.98e-218
Identity = 327/382 (85.60%), Postives = 348/382 (91.10%), Query Frame = 0

Query: 1   MPIFAATTLGSLSAHSFLSLLASTDASHSLSSSFVSTH---KSPSKRPSNFAARVSLSGK 60
           MPIF AT + S+S HSFLSLLAST  + S SSS  S+    KSPSKRPS F +RVSLSGK
Sbjct: 45  MPIFLAT-IASVSTHSFLSLLASTSDASSTSSSSSSSILPLKSPSKRPSIFPSRVSLSGK 104

Query: 61  PEPIAGVLESSPSSPESVRRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLV 120
           P+PIAGVL+   +SPESVRRARRSADWK AREYLDSGFI++GRIEGSNAGGLLVRFYSL+
Sbjct: 105 PDPIAGVLD---TSPESVRRARRSADWKAAREYLDSGFIYEGRIEGSNAGGLLVRFYSLM 164

Query: 121 GFLPFPLLSPAHSCKEPYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFS 180
           GFLPFP LSP+HSCKEP KSIQDIAKSL GSLI VKVIQADE+NK LIFSEKEA WSKFS
Sbjct: 165 GFLPFPQLSPSHSCKEPNKSIQDIAKSLTGSLISVKVIQADERNKKLIFSEKEATWSKFS 224

Query: 181 EKVGVGDVYEARVGSVEDYGAFVHLRFSDGLYHLTGLVHISEVSWDLVQDVRDILSEGDE 240
            +VGVGDVYEA+VGS+EDYGAFVHLRFSDGLYHLTGLVH+SEVSWDLVQDVRDILSEGDE
Sbjct: 225 GQVGVGDVYEAKVGSLEDYGAFVHLRFSDGLYHLTGLVHVSEVSWDLVQDVRDILSEGDE 284

Query: 241 VRVKVIDVDRDKSRITLSIKQLEEDPLLETLDKVIPQDDSAEPDSFGPKSDSEIIPLPGL 300
           V VKVI+VDRDKSRITLSI+QLEEDPLLETLDKVIPQD SAEPDSFGPKSDSEIIPLPGL
Sbjct: 285 VTVKVINVDRDKSRITLSIRQLEEDPLLETLDKVIPQDSSAEPDSFGPKSDSEIIPLPGL 344

Query: 301 DTIFEELLQEEGIEDVHINRQGFEKRVVSQDLQLWLSNAPPVEKKFTLLARAGRQVQEIQ 360
            TI EEL QEEGI DV +NRQGFEKRVVSQDLQLWLSNAPP+EKKFTLLARAGRQVQEIQ
Sbjct: 345 GTIIEELQQEEGIVDVRVNRQGFEKRVVSQDLQLWLSNAPPIEKKFTLLARAGRQVQEIQ 404

Query: 361 LTTSLDQEGIKRALQRVLERVP 379
           LTTSLDQEGIKRALQRVLERVP
Sbjct: 405 LTTSLDQEGIKRALQRVLERVP 422

BLAST of Csor.00g133540 vs. ExPASy TrEMBL
Match: A0A0A0KTX2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G012510 PE=4 SV=1)

HSP 1 Score: 608 bits (1567), Expect = 3.99e-217
Identity = 328/382 (85.86%), Postives = 350/382 (91.62%), Query Frame = 0

Query: 1   MPIFAATTLGSLSAHSFLSLLAST-DASH--SLSSSFVSTHKSPSKRPSNFAARVSLSGK 60
           MPIF AT + S+SAHSFLSLLAST DAS   S SSSF+   KSPSKR S F +RVSLSGK
Sbjct: 1   MPIFVAT-IASVSAHSFLSLLASTSDASSTSSSSSSFILPLKSPSKRSSIFPSRVSLSGK 60

Query: 61  PEPIAGVLESSPSSPESVRRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLV 120
           P+PIAGVL+   +SPESVRRARRSADWK AREYLDSGFI++GRIEGSNAGGLLVRFYSLV
Sbjct: 61  PDPIAGVLD---TSPESVRRARRSADWKAAREYLDSGFIYEGRIEGSNAGGLLVRFYSLV 120

Query: 121 GFLPFPLLSPAHSCKEPYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFS 180
           GFLPFP LSP+HSCKEPYKSIQDIAKSLIGSLI VKVIQADEKN+ LIFSEKEAA SKFS
Sbjct: 121 GFLPFPQLSPSHSCKEPYKSIQDIAKSLIGSLISVKVIQADEKNRKLIFSEKEAARSKFS 180

Query: 181 EKVGVGDVYEARVGSVEDYGAFVHLRFSDGLYHLTGLVHISEVSWDLVQDVRDILSEGDE 240
            +V VGDVYE +VGSVEDYGAFVHLR SDGLYHLTGLVH+SEVSWDLVQDVRDILSEGDE
Sbjct: 181 GQVAVGDVYEGKVGSVEDYGAFVHLRLSDGLYHLTGLVHVSEVSWDLVQDVRDILSEGDE 240

Query: 241 VRVKVIDVDRDKSRITLSIKQLEEDPLLETLDKVIPQDDSAEPDSFGPKSDSEIIPLPGL 300
           V VKVI+V+++KSRITLSI+QLEEDPLLETLDKVIPQ+ SAEPDSFGPK DSEIIPLPGL
Sbjct: 241 VTVKVINVNKNKSRITLSIRQLEEDPLLETLDKVIPQESSAEPDSFGPKGDSEIIPLPGL 300

Query: 301 DTIFEELLQEEGIEDVHINRQGFEKRVVSQDLQLWLSNAPPVEKKFTLLARAGRQVQEIQ 360
           +TI EELLQEEGI DV +NRQGFEKRVVSQDLQLWLSNAPPVEKKFTLLARAGRQVQEIQ
Sbjct: 301 ETIIEELLQEEGIVDVRVNRQGFEKRVVSQDLQLWLSNAPPVEKKFTLLARAGRQVQEIQ 360

Query: 361 LTTSLDQEGIKRALQRVLERVP 379
           LTTSLDQEGIKRALQRVLERVP
Sbjct: 361 LTTSLDQEGIKRALQRVLERVP 378

BLAST of Csor.00g133540 vs. ExPASy TrEMBL
Match: A0A6J1CUJ6 (uncharacterized protein LOC111014714 OS=Momordica charantia OX=3673 GN=LOC111014714 PE=4 SV=1)

HSP 1 Score: 607 bits (1564), Expect = 1.10e-216
Identity = 318/380 (83.68%), Postives = 346/380 (91.05%), Query Frame = 0

Query: 1   MPIFAATTLGSLSAHSFLSLLASTDASHSLSSSFVSTHKSPSKRPSNFAARVSLSGKPEP 60
           MPI  AT LGSLS +SFLS  ASTD+S S  S     H SPSKR  NF  R+SL   P+P
Sbjct: 1   MPICTAT-LGSLSPYSFLSHFASTDSSPS--SILTHNHNSPSKRSPNFPPRLSLPANPDP 60

Query: 61  IAGVLES-SPSSPESVRRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLVGF 120
           IAGVL++ SP+SPES+ R+RRS DWK AREYLDSGFI++GRIEGSNAGGLLVRF+SLVGF
Sbjct: 61  IAGVLDTTSPASPESLPRSRRSEDWKAAREYLDSGFIYEGRIEGSNAGGLLVRFHSLVGF 120

Query: 121 LPFPLLSPAHSCKEPYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFSEK 180
           LPFP LSP+HSCKEPYKSIQDIAKSL+GS++PVK+IQADE+NK LIFSEKEAAWSKFSE+
Sbjct: 121 LPFPQLSPSHSCKEPYKSIQDIAKSLLGSIVPVKIIQADERNKKLIFSEKEAAWSKFSEQ 180

Query: 181 VGVGDVYEARVGSVEDYGAFVHLRFSDGLYHLTGLVHISEVSWDLVQDVRDILSEGDEVR 240
           V VG+VY+ARVGSVEDYGAFVHLRFSDGLYHLTGLVH+SEVSWDLVQDVRDILSEGDEVR
Sbjct: 181 VSVGEVYDARVGSVEDYGAFVHLRFSDGLYHLTGLVHVSEVSWDLVQDVRDILSEGDEVR 240

Query: 241 VKVIDVDRDKSRITLSIKQLEEDPLLETLDKVIPQDDSAEPDSFGPKSDSEIIPLPGLDT 300
           VKVI+VDR+KSRITLSIKQLEEDPLLETLDKVIPQD SAEPDSFGP+SDSEIIPLPGL+T
Sbjct: 241 VKVINVDREKSRITLSIKQLEEDPLLETLDKVIPQDGSAEPDSFGPRSDSEIIPLPGLET 300

Query: 301 IFEELLQEEGIEDVHINRQGFEKRVVSQDLQLWLSNAPPVEKKFTLLARAGRQVQEIQLT 360
           IFEELLQE+GIEDV +NRQGFEKRVVSQDLQLWLSNAPPVEKKF LLARAGRQVQEIQLT
Sbjct: 301 IFEELLQEDGIEDVRVNRQGFEKRVVSQDLQLWLSNAPPVEKKFILLARAGRQVQEIQLT 360

Query: 361 TSLDQEGIKRALQRVLERVP 379
           TSLDQEGIK ALQ VLERVP
Sbjct: 361 TSLDQEGIKMALQHVLERVP 377

BLAST of Csor.00g133540 vs. TAIR 10
Match: AT3G23700.1 (Nucleic acid-binding proteins superfamily )

HSP 1 Score: 425.6 bits (1093), Expect = 4.1e-119
Identity = 237/392 (60.46%), Postives = 282/392 (71.94%), Query Frame = 0

Query: 1   MPIFA-ATTLGSLS--AHSF----------LSLLASTDASHSLSSSFVSTHKSPSKRPSN 60
           M +F+ ATTLGS+S  +H F          L LL S+ +S S  +S V   KS S   + 
Sbjct: 1   MAVFSGATTLGSVSFASHLFDQQSTFLSCPLRLLPSSSSSSSNRNSLVCIVKSFSSSATA 60

Query: 61  FAARVSLSGKPEPIAGVLESSPSSPESVRRARRSADWKTAREYLDSGFIFKGRIEGSNAG 120
              R S       +     S          A   +DWKTA+ Y  SG  F+G ++G N G
Sbjct: 61  DTDRNSDQSASSSVLSASNSLLRDTSDEASAAGPSDWKTAKAYCKSGDTFEGEVQGFNGG 120

Query: 121 GLLVRFYSLVGFLPFPLLSPAHSCKEPYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFS 180
           GLL+RF+SLVGFLP+P LSP+ SCKEP KSI +IAK+L+GS +PVKV+QADE+N+ LI S
Sbjct: 121 GLLIRFHSLVGFLPYPQLSPSRSCKEPQKSIHEIAKTLVGSKLPVKVVQADEENRKLILS 180

Query: 181 EKEAAWSKFSEKVGVGDVYEARVGSVEDYGAFVHLRFSDGLYHLTGLVHISEVSWDLVQD 240
           EK A W K+S+ V VGDV+  RVGSVEDYGAF+HLRF DGLYHLTGLVH+SEVSWD VQD
Sbjct: 181 EKLALWPKYSQNVNVGDVFNGRVGSVEDYGAFIHLRFDDGLYHLTGLVHVSEVSWDYVQD 240

Query: 241 VRDILSEGDEVRVKVIDVDRDKSRITLSIKQLEEDPLLETLDKVIPQDDSAEPDSFGPKS 300
           VRD+L +GDEVRV V ++D++KSRITLSIKQLE+DPLLETLDKVI +D S    S    +
Sbjct: 241 VRDVLRDGDEVRVIVTNIDKEKSRITLSIKQLEDDPLLETLDKVILKDSSTGSPSLSSNN 300

Query: 301 DSEIIPLPGLDTIFEELLQEEGIEDVHINRQGFEKRVVSQDLQLWLSNAPPVEKKFTLLA 360
              I PLPGL+TI EELL+E+GIE V INRQGFEKRVVSQDLQLWLSN PP + KF LLA
Sbjct: 301 GDTIEPLPGLETILEELLKEDGIEAVKINRQGFEKRVVSQDLQLWLSNTPPSDGKFVLLA 360

Query: 361 RAGRQVQEIQLTTSLDQEGIKRALQRVLERVP 380
           RAGRQVQEI LTTSL+Q GIK+ALQ VLERVP
Sbjct: 361 RAGRQVQEIHLTTSLEQGGIKKALQHVLERVP 392

BLAST of Csor.00g133540 vs. TAIR 10
Match: AT5G30510.1 (ribosomal protein S1 )

HSP 1 Score: 98.2 bits (243), Expect = 1.5e-20
Identity = 73/248 (29.44%), Postives = 125/248 (50.40%), Query Frame = 0

Query: 24  TDASHSL------SSSFVSTHKSPSKRPSNFAARVSLSGKPEP--IAGVLESSPSSPESV 83
           TDA+ +L      SS+++S  ++   R  +      + G  E   I G  ES  S   S+
Sbjct: 109 TDANGALVDISAKSSAYLSVEQACIHRIKHVEEAGIVPGMVEEFVIIGENESDDSLLLSL 168

Query: 84  RRARRSADWKTAREYLDSGFIFKGRIEGSNAGGLLVRFYSLVGFLPFPLLSPAHSCKEPY 143
           R  +    W+  R+      I K ++ G+N GGL+     L GF+PF  +S   + +E  
Sbjct: 169 RNIQYELAWERCRQLQAEDVIVKAKVIGANKGGLVALVEGLRGFVPFSQISSKAAAEE-- 228

Query: 144 KSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEAAWSKFSEKVGVGDVYEARVGSVED 203
                    L+   IP+K ++ DE+   L+ S ++A  +    ++G+G V    V S++ 
Sbjct: 229 ---------LLEKEIPLKFVEVDEEQTKLVLSNRKAV-ADSQAQLGIGSVVLGVVQSLKP 288

Query: 204 YGAFVHLRFSDGLYHLTGLVHISEVSWDLVQDVRDILSEGDEVRVKVIDVDRDKSRITLS 263
           YGAF+ +        + GL+H+S++S D V D+  +L  GD ++V ++  DRD+ R++LS
Sbjct: 289 YGAFIDIG------GINGLLHVSQISHDRVSDIATVLQPGDTLKVMILSHDRDRGRVSLS 338

BLAST of Csor.00g133540 vs. TAIR 10
Match: AT1G12800.1 (Nucleic acid-binding, OB-fold-like protein )

HSP 1 Score: 95.9 bits (237), Expect = 7.4e-20
Identity = 79/249 (31.73%), Postives = 120/249 (48.19%), Query Frame = 0

Query: 139 QDIAKSLIGSLIPVKVIQADEKNKNLIFS----EKEAAWSK---FSEKVGVGDVYEARVG 198
           Q    S +G  I V V+ A+  ++ LIFS    E E    K      K+ VGDV +  + 
Sbjct: 531 QKFLSSFVGQKIKVNVVMANRNSRKLIFSMRPRENEEEVEKKRTLMAKLRVGDVVKCCIK 590

Query: 199 SVEDYGAFVHLRFSDGLYHLTGLVHISEVSWDLVQDVRDILSEGDEVRVKVIDVDRDKSR 258
            +  +G F  L        +  LVH SEVSWD   D       G  V  KV  +D    R
Sbjct: 591 KITYFGIFCELE------GVPALVHQSEVSWDATLDPASYFKIGQIVEAKVHQLDFALER 650

Query: 259 ITLSIKQLEEDPLLETLDKVIPQDDSAEPDSFGPKSDSEIIPL--PGLDTIFEELLQEEG 318
           I LS+K++  DPL E L+ V+  D+    D  G +  +  +    P ++++ +EL   EG
Sbjct: 651 IFLSLKEITPDPLTEALESVVGGDN----DQLGGRLQAAELDAEWPDVESLIKELEMVEG 710

Query: 319 IEDVHINRQGFEKRVVSQDLQLWLSNAPPVEKKFTLLARAGRQVQEIQLTTSLDQEGIKR 378
           I+ V  +R  F    ++   Q+++  AP  E ++ LLARAG +VQE+ +  SL +E +K 
Sbjct: 711 IQSVSKSR-FFLSPGLAPTFQVYM--APMFENQYKLLARAGNRVQELIVEASLSKEEMKS 766

BLAST of Csor.00g133540 vs. TAIR 10
Match: AT3G11964.1 (RNA binding;RNA binding )

HSP 1 Score: 54.3 bits (129), Expect = 2.5e-07
Identity = 37/133 (27.82%), Postives = 68/133 (51.13%), Query Frame = 0

Query: 132  KEPYKSIQDIAKSLIGSLIPVKVIQADEKNKNLIFSEKEA-------AWSKFSEKVGVGD 191
            KEP K         +G L+  +V+  +  +K +  + K         + S   +K+ VGD
Sbjct: 1383 KEPEKEFP------VGKLVTGRVLNVEPLSKRIEVTLKTVNAGGRPKSESYDLKKLHVGD 1442

Query: 192  VYEARVGSVEDYGAFVHLRFSDGLYHLTGLVHISEVSWDLVQDVRDILSEGDEVRVKVID 251
            +   R+  VE +G F+ +  +     + GL HIS++S D +++V+     G+ VR K++ 
Sbjct: 1443 MISGRIRRVEPFGLFIDIDQTG----MVGLCHISQLSDDRMENVQARYKAGESVRAKILK 1502

Query: 252  VDRDKSRITLSIK 258
            +D +K RI+L +K
Sbjct: 1503 LDEEKKRISLGMK 1505

BLAST of Csor.00g133540 vs. TAIR 10
Match: AT4G29060.1 (elongation factor Ts family protein )

HSP 1 Score: 53.5 bits (127), Expect = 4.2e-07
Identity = 27/88 (30.68%), Postives = 58/88 (65.91%), Query Frame = 0

Query: 177 SEKVGVGDVYEARVGSVEDYGAFVHL-RFSDGLYHLTGLVHISEVSWDLVQDVRDILSEG 236
           +E++  G  +  +V +++ +GAFV    F+D      GLVH+S++S + V+DV  +++ G
Sbjct: 130 NEELVPGATFTGKVRAIQPFGAFVDFGAFTD------GLVHVSQLSDNFVKDVSSVVTIG 189

Query: 237 DEVRVKVIDVDRDKSRITLSIKQLEEDP 264
            EV+V++++ D +  RI+L++++ ++ P
Sbjct: 190 QEVKVRLVEADIESKRISLTMRENDDPP 211

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P293441.7e-2130.5430S ribosomal protein S1, chloroplastic OS=Spinacia oleracea OX=3562 GN=RPS1 PE=... [more]
P462285.5e-2030.0030S ribosomal protein S1 OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SA... [more]
Q93VC72.1e-1929.4430S ribosomal protein S1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RPS1 ... [more]
Q9JZ442.7e-1932.9930S ribosomal protein S1 OS=Neisseria meningitidis serogroup B (strain MC58) OX=... [more]
P735308.0e-1930.5330S ribosomal protein S1 homolog A OS=Synechocystis sp. (strain PCC 6803 / Kazus... [more]
Match NameE-valueIdentityDescription
KAG6582039.11.45e-263100.0030S ribosomal protein S1, chloroplastic, partial [Cucurbita argyrosperma subsp. ... [more]
XP_022979820.13.98e-26198.94uncharacterized protein LOC111479406 [Cucurbita maxima][more]
KAG7018465.15.42e-25998.4230S ribosomal protein S1, chloroplastic [Cucurbita argyrosperma subsp. argyrospe... [more]
XP_022955545.18.68e-25898.15LOW QUALITY PROTEIN: uncharacterized protein LOC111457527 [Cucurbita moschata][more]
XP_023526022.13.53e-25797.89LOW QUALITY PROTEIN: uncharacterized protein LOC111789621 [Cucurbita pepo subsp.... [more]
Match NameE-valueIdentityDescription
A0A6J1IUF31.93e-26198.94uncharacterized protein LOC111479406 OS=Cucurbita maxima OX=3661 GN=LOC111479406... [more]
A0A6J1GVD74.20e-25898.15LOW QUALITY PROTEIN: uncharacterized protein LOC111457527 OS=Cucurbita moschata ... [more]
A0A1S3BXL42.98e-21885.6030S ribosomal protein S1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494553 PE=4... [more]
A0A0A0KTX23.99e-21785.86Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G012510 PE=4 SV=1[more]
A0A6J1CUJ61.10e-21683.68uncharacterized protein LOC111014714 OS=Momordica charantia OX=3673 GN=LOC111014... [more]
Match NameE-valueIdentityDescription
AT3G23700.14.1e-11960.46Nucleic acid-binding proteins superfamily [more]
AT5G30510.11.5e-2029.44ribosomal protein S1 [more]
AT1G12800.17.4e-2031.73Nucleic acid-binding, OB-fold-like protein [more]
AT3G11964.12.5e-0727.82RNA binding;RNA binding [more]
AT4G29060.14.2e-0730.68elongation factor Ts family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022967RNA-binding domain, S1SMARTSM00316S1_6coord: 181..257
e-value: 3.1E-20
score: 83.2
coord: 92..169
e-value: 5.4E-4
score: 29.3
NoneNo IPR availableGENE3D2.40.50.140coord: 84..175
e-value: 4.5E-8
score: 35.1
coord: 176..269
e-value: 3.5E-26
score: 93.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 59..79
NoneNo IPR availablePANTHERPTHR47559OS03G0844900 PROTEINcoord: 13..379
IPR003029S1 domainPFAMPF00575S1coord: 180..257
e-value: 4.6E-19
score: 68.5
IPR003029S1 domainPROSITEPS50126S1coord: 94..169
score: 12.079132
IPR003029S1 domainPROSITEPS50126S1coord: 183..257
score: 20.898582
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 170..262
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 92..169

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g133540.m01Csor.00g133540.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032508 DNA duplex unwinding
biological_process GO:0000481 maturation of 5S rRNA
biological_process GO:0009737 response to abscisic acid
biological_process GO:0034337 RNA folding
cellular_component GO:0009507 chloroplast
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005840 ribosome
molecular_function GO:0003729 mRNA binding
molecular_function GO:0003676 nucleic acid binding