Csor.00g133450 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g133450
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionRibonuclease
LocationCsor_Chr14: 13389349 .. 13392752 (+)
RNA-Seq ExpressionCsor.00g133450
SyntenyCsor.00g133450
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTTCGCAAGATCCACTCCCGAAATGGGCATCGAAGCCTTGTGTAATGGGCATCGACGAAGCTGGTCGAGGCCCTGTTTTAGGTTTTCTATTTTCCTATTCGATTCCTTCCATTTCTGCATCTTTAACACCGCTCCGATTATCTTATTTTCGGATATGATTTAGTTTACCAATTTGTTTCTGAAATGTTACTGCATTTGATCTCAGGGCCAATGGTGTATGGATGCTTGTACTGCACTCGTTCCTATTTGAAAGAACTTTCGTCTTTGAATTTTGCAGGTTTAGCTTCTCTGCAAAATTGATGGTTCTACCGATTTTCTTTTAAGTGTTTTTCATTGTTTCTCAGTTTCGTTTACTTTTCTTCCAAATTTTTATGTTTTGTTTTGCATCTAGACTCAAAAACATTAAAAGAGGAGAAGAGGGAGGAATTGTTTGAAGGATTGAAGACTAATGAGTCAATTGGATGGGCTGTTGATGTCATTGATCCTCGGGAACTTTCATCCAAAATGCTAAATAAGTCAGTATTTGGTGGTTTATAAATTCTTTCATTTGCACAAACACTCTAAGCTAACGGATAACGATGCTTTAACAACGGAAGGCAAAATGTTAAGTCCAACATAATGTATCATATGAGAACTTTCACGCCTGGGTTTTGTTCTGAAGTTCTTCTTTTTATTGTTCAACAGAGTAAAGATAAACCTAAATGAAATATCACATGACTCTGCAATGGGTCTGGTTACCAAGGTTCTAGGCATGGGAGTTCTTTTAACTGAGGTAACTCTCTCCCTGGTTTCTTGCATTCATGAGTCAGTTATGCGATTTTAATATAGTATTTCTTTCAACAAGAAATGAAATTGCCATTGATTGATGAAAAGAAATAGAAATTGTTAAGAACACACTCTCTCAACAGAGAAAGCAGAGGCAACAACTTGAGAAGATTGGATAGCCTTCGGTAACTTCCAGTTATTTTTTGTATGCCTTTGTATATTCGTTCATGAGAGTTAGCAGGATGCAACACGTTCTTTATGAGGAAGATCTGTAAAGATTAAATTTTGGAAAGTTATATTCATGATTTATTCAGGTCCATAAGATATAGGGAAAGAAAATCCCCATTCAGCATATCTCATCTTCCATTGCCTTCAAACTTTTTTGTTATCAATTGATGATTTGAAGTCATTTGCAGATTTGTTCTGCCCCAAGAACAATGTTAGATCAATCACTTCTATTTATTTTCAGGTTTATGTGGATACTGTTGGAGATCCTGAGAAGTACAGAATCAAGTTGTCTGAAAAATTTCCCAATGTAAAATTTGTAGTTGCAAAGAAAGCTGATAGTTTATATCCTGTTGTCAGTGGGGCAAGCATAGTCGCAAAGGTAATGCTAATGCGTCCTTTTAAAATCTGGCATTCCAATCGTTTCCCACACTTAAATTCATCATTTTGACTTGTAGGTGACAAGAGATCGTGTGTTGCGGGATTGGGCATTGGAGGAAACAGCTGAAAACATGCATAGAAACTTTGGTTCTGGATACCCTGGAGGTCAATTTCATCCTATAAATCTTTTTTGCAGCATACTATAATCACGTTTTCACTGCACTGGGTGAAATCAATAGTGCTCTTATGATTTAATCTATGTAGATCCAACAACTAAATGTTGGTTAGAAGATCACAAGCACTCGGTATTTGGGTTCCCCACATTGGTCCGCTTCAGTTGGGGTACCTGCACTTCATATTTCAAAGACATTGTTGAAGTATCATGGTTAGTTTATACTCTGCCTTGCAAGTATTATAGCTCTTTGCAGAATTAGCTCATTATGCCTTATACGTTTCTTTCATGTTCTCCACGTTATAGAAAGGACAGGCTTTGTTGTTTAAAGTGCTATACATTTTCTTTTATGTTCTCCACGGTATAGAAAGGACAGGCTTTGTTATTTAAAGTGCTATACATTTTCTTTTAGCTCATTATCCCCTATAGGACTAGGTATGTTGCTTAAAGTGCGTTTGTTATTGGTGGTGGGATTGATTTAATGAAAGATGGGGTTGATTTAATGAAAGCAGTCTTAAAAACAATTTTGAATATCATATGGAGTTTAGTAATTAATGATGGCAATTGTTTATTGATGTAAGGCACTTCGCATTTTTTGCTGGATCATCATCCTGCTCTGATGTACTGGGATGATCGGTTCTTTCCCATTCTAAATTAAAACTTGATTTGTTCACTGAAAATGAATGATTCTACCTTTATGCTAGACTATAGTTTTGTTTGGTGCATCCCACTTTTATTGTACAATAGTTAGATATCCATTTCGGTTCGTTCTTCGGGTCTACTTCTTGATACACCCACGTTCAGTGTTTCTTTTAACATTAGTGAGTGTTTAGACTCTAATCTCACAGGGAAAAACCCTACAACATTTAGTTGTCAAGAAACTCACAAGATTATAATATTCTAGGTAAGTGACCATGACCTCCATACAATTTGAACCCATTCTTCAAAAGCCTTTGCCGCTTGGCCAACCTAGGATGGCTATGCCTAGTTCCTGTTCATCAATTTAGCTTCTCATAAATCATCAAATAAGCATAACATGATCCATTTGTTTACATAGGAGTGGCCCAAGCCCACCGCTTTGTCTTCTTTGGGCTTTACCTCAATGTTTATAAAACACGTCTGTTAAAGAGAGGTTTCCACACCCTTATAAAGAGTGTGTTTCATTCTCCTCCCCAACTGATGTGGGATCTCACAATCTACCTCCTTTGGGGCCTAGCGTCCTTGTTGGCACCCGTTTCTTTCTCCAATCAATGTTGGACCCCCCCCAAATCCACCCCCCTTCGAGGCCTAGCGTCCTTGCTAGCACATCACCTCATGTACACCTCCCTTCGGAGCTCAGCCTCCTCACTGACACATCGCTTGGTGTTTGGCTATAATACCATTTGTAACGGCCCAAACCCACTGCTAGCAGATATTGTCCTCTTTGGACTTTACGTTTCGGGCTTCCCCACAAGATTTTTAAAACGCGTATGCTATGGAGAGGTTTCCACACCCTTATAAAGGGTGTTTCATTCTCCTCCCCAACCGATGTGGGATTTTACAAAATTAGATGCCGAAACTTCTCATACTTCCTATACTTCCTTGACACTAACTCAAACTTTAAAGTTGTTATGGAAGTTGTTTTGATTATGGAATCACTATGATGCAAGTTTTGCTTTTTCTATGTGCTTGATGTCCAGGGAATCAGACAAAGTGGATGAAGATGGGGCAACGGGTGGAAGTGGTAAGAAACAATTAAGACTAAGCAACGTTGGTATCACCACATCCAAGAGAAAGAGTGAAGAAATTGAATCAAATGCGAAAGGACGGTGCAAATTTTTCCAGGCTAGAAAACTTGAACAACTCACTCAATTCTGA

mRNA sequence

ATGTCTTCGCAAGATCCACTCCCGAAATGGGCATCGAAGCCTTGTGTAATGGGCATCGACGAAGCTGGTCGAGGCCCTGTTTTAGGGCCAATGGTGTATGGATGCTTGTACTGCACTCGTTCCTATTTGAAAGAACTTTCGTCTTTGAATTTTGCAGACTCAAAAACATTAAAAGAGGAGAAGAGGGAGGAATTGTTTGAAGGATTGAAGACTAATGAGTCAATTGGATGGGCTGTTGATGTCATTGATCCTCGGGAACTTTCATCCAAAATGCTAAATAAAGTAAAGATAAACCTAAATGAAATATCACATGACTCTGCAATGGGTCTGGTTACCAAGGTTCTAGGCATGGGAGTTCTTTTAACTGAGGTTTATGTGGATACTGTTGGAGATCCTGAGAAGTACAGAATCAAGTTGTCTGAAAAATTTCCCAATGTAAAATTTGTAGTTGCAAAGAAAGCTGATAGTTTATATCCTGTTGTCAGTGGGGCAAGCATAGTCGCAAAGGTGACAAGAGATCGTGTGTTGCGGGATTGGGCATTGGAGGAAACAGCTGAAAACATGCATAGAAACTTTGGTTCTGGATACCCTGGAGATCCAACAACTAAATGTTGGTTAGAAGATCACAAGCACTCGGTATTTGGGTTCCCCACATTGGTCCGCTTCAGTTGGGGTACCTGCACTTCATATTTCAAAGACATTGTTGAAGTATCATGGGAATCAGACAAAGTGGATGAAGATGGGGCAACGGGTGGAAGTGGTAAGAAACAATTAAGACTAAGCAACGTTGGTATCACCACATCCAAGAGAAAGAGTGAAGAAATTGAATCAAATGCGAAAGGACGGTGCAAATTTTTCCAGGCTAGAAAACTTGAACAACTCACTCAATTCTGA

Coding sequence (CDS)

ATGTCTTCGCAAGATCCACTCCCGAAATGGGCATCGAAGCCTTGTGTAATGGGCATCGACGAAGCTGGTCGAGGCCCTGTTTTAGGGCCAATGGTGTATGGATGCTTGTACTGCACTCGTTCCTATTTGAAAGAACTTTCGTCTTTGAATTTTGCAGACTCAAAAACATTAAAAGAGGAGAAGAGGGAGGAATTGTTTGAAGGATTGAAGACTAATGAGTCAATTGGATGGGCTGTTGATGTCATTGATCCTCGGGAACTTTCATCCAAAATGCTAAATAAAGTAAAGATAAACCTAAATGAAATATCACATGACTCTGCAATGGGTCTGGTTACCAAGGTTCTAGGCATGGGAGTTCTTTTAACTGAGGTTTATGTGGATACTGTTGGAGATCCTGAGAAGTACAGAATCAAGTTGTCTGAAAAATTTCCCAATGTAAAATTTGTAGTTGCAAAGAAAGCTGATAGTTTATATCCTGTTGTCAGTGGGGCAAGCATAGTCGCAAAGGTGACAAGAGATCGTGTGTTGCGGGATTGGGCATTGGAGGAAACAGCTGAAAACATGCATAGAAACTTTGGTTCTGGATACCCTGGAGATCCAACAACTAAATGTTGGTTAGAAGATCACAAGCACTCGGTATTTGGGTTCCCCACATTGGTCCGCTTCAGTTGGGGTACCTGCACTTCATATTTCAAAGACATTGTTGAAGTATCATGGGAATCAGACAAAGTGGATGAAGATGGGGCAACGGGTGGAAGTGGTAAGAAACAATTAAGACTAAGCAACGTTGGTATCACCACATCCAAGAGAAAGAGTGAAGAAATTGAATCAAATGCGAAAGGACGGTGCAAATTTTTCCAGGCTAGAAAACTTGAACAACTCACTCAATTCTGA

Protein sequence

MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEEKREELFEGLKTNESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVLLTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTQF
Homology
BLAST of Csor.00g133450 vs. ExPASy Swiss-Prot
Match: Q9SEZ6 (Ribonuclease H2 subunit A OS=Arabidopsis thaliana OX=3702 GN=At2g25100 PE=2 SV=2)

HSP 1 Score: 446.4 bits (1147), Expect = 2.5e-124
Identity = 212/297 (71.38%), Postives = 257/297 (86.53%), Query Frame = 0

Query: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60
           M S+   P+WAS+PC+MGIDEAGRGPVLGPMVYGC+YC  SY   L+SL+FADSKTLKEE
Sbjct: 1   MESECLTPEWASQPCLMGIDEAGRGPVLGPMVYGCMYCPISYQSSLASLHFADSKTLKEE 60

Query: 61  KREELFEGLKTNESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120
           KREEL+E LK ++S+GWAVDVIDPRELS+KML K K NLNEISH+SAMGL+ +VL MGVL
Sbjct: 61  KREELYESLKLDKSLGWAVDVIDPRELSAKMLAKNKTNLNEISHNSAMGLIKRVLDMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180
           LTE Y+DTVGDP+KYRIKLSE+FP++KFVV+KKADSL+P+VSGASIVAKVTRDR L++W 
Sbjct: 121 LTEAYLDTVGDPDKYRIKLSERFPSIKFVVSKKADSLFPIVSGASIVAKVTRDRALKEWL 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240
           +EET E+++RNFGSGYPGDP TK WL  HKHSVFGFP+LVRFSWGTCT++ K  VEV+WE
Sbjct: 181 VEETGEDINRNFGSGYPGDPETKAWLVQHKHSVFGFPSLVRFSWGTCTTHLKGEVEVAWE 240

Query: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTQF 298
           +D+ +E G  G S K+Q +LS+ G  T +++SEEIES+ KGRCKF QARK++QLTQF
Sbjct: 241 ADENEESG-NGSSSKRQAKLSSFGFKTCEKRSEEIESSGKGRCKFLQARKIQQLTQF 296

BLAST of Csor.00g133450 vs. ExPASy Swiss-Prot
Match: Q5U209 (Ribonuclease H2 subunit A OS=Rattus norvegicus OX=10116 GN=Rnaseh2a PE=2 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 2.3e-66
Identity = 120/245 (48.98%), Postives = 165/245 (67.35%), Query Frame = 0

Query: 6   PLPKWASK-PCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEEKREE 65
           P+P    K PCV+G+DEAGRGPVLGPMVY   YC  S L +L +L  ADSKTL E +RE 
Sbjct: 19  PVPAVCLKEPCVLGVDEAGRGPVLGPMVYAICYCPLSRLADLEALKVADSKTLTENERER 78

Query: 66  LFEGLKTN-ESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVLLTE 125
           LF  ++ + + +GWA+D++ P  +S+ ML +VK NLN +SHD+A GL+   +   V +T+
Sbjct: 79  LFAKMEEDGDFVGWALDILSPNLISTSMLGRVKYNLNSMSHDTAAGLIQHAMDQNVKVTQ 138

Query: 126 VYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWALEE 185
           V+VDTVG PE Y+ +L ++FP ++  V  KADSL+PVVS ASI+AKV RD+ +++W   E
Sbjct: 139 VFVDTVGMPETYQARLQQRFPGIEVTVKAKADSLFPVVSAASIIAKVARDQAVKNWQFVE 198

Query: 186 TAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYF-KDIVEVSWESD 245
           + + +  ++GSGYP DP TK WL  H   VFGFP  VRFSW T  +   K+   V+WE  
Sbjct: 199 SLQGLDSDYGSGYPNDPKTKAWLRKHVDPVFGFPQFVRFSWSTAQAILEKEAESVTWEDS 258

Query: 246 KVDED 248
             +ED
Sbjct: 259 AAEED 263

BLAST of Csor.00g133450 vs. ExPASy Swiss-Prot
Match: Q9CWY8 (Ribonuclease H2 subunit A OS=Mus musculus OX=10090 GN=Rnaseh2a PE=1 SV=2)

HSP 1 Score: 252.7 bits (644), Expect = 5.2e-66
Identity = 122/245 (49.80%), Postives = 164/245 (66.94%), Query Frame = 0

Query: 6   PLPKWASK-PCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEEKREE 65
           P+P    K PCV+G+DEAGRGPVLGPMVY   YC  S L +L +L  ADSKTL E +RE 
Sbjct: 19  PVPAVCLKEPCVLGVDEAGRGPVLGPMVYAICYCPLSRLADLEALKVADSKTLTENERER 78

Query: 66  LFEGLKTN-ESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVLLTE 125
           LF  ++ + + +GWA+DV+ P  +S+ ML +VK NLN +SHD+A GL+   L   V +T+
Sbjct: 79  LFAKMEEDGDFVGWALDVLSPNLISTSMLGRVKYNLNSLSHDTAAGLIQYALDQNVNVTQ 138

Query: 126 VYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWALEE 185
           V+VDTVG PE Y+ +L + FP ++  V  KADSL+PVVS ASI AKV RD+ +++W   E
Sbjct: 139 VFVDTVGMPETYQARLQQHFPGIEVTVKAKADSLFPVVSAASIFAKVARDKAVKNWQFVE 198

Query: 186 TAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYF-KDIVEVSWESD 245
             +++  ++GSGYP DP TK WL  H   VFGFP  VRFSW T  +   K+  +V WE  
Sbjct: 199 NLQDLDSDYGSGYPNDPKTKAWLRKHVDPVFGFPQFVRFSWSTAQAILEKEAEDVIWEDS 258

Query: 246 KVDED 248
           + +ED
Sbjct: 259 EAEED 263

BLAST of Csor.00g133450 vs. ExPASy Swiss-Prot
Match: O75792 (Ribonuclease H2 subunit A OS=Homo sapiens OX=9606 GN=RNASEH2A PE=1 SV=2)

HSP 1 Score: 250.4 bits (638), Expect = 2.6e-65
Identity = 130/292 (44.52%), Postives = 180/292 (61.64%), Query Frame = 0

Query: 6   PLPKWASK-PCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEEKREE 65
           P+P    K PCV+G+DEAGRGPVLGPMVY   YC    L +L +L  ADSKTL E +RE 
Sbjct: 19  PVPAVCRKEPCVLGVDEAGRGPVLGPMVYAICYCPLPRLADLEALKVADSKTLLESERER 78

Query: 66  LFEGLKTNESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVLLTEV 125
           LF  ++  + +GWA+DV+ P  +S+ ML +VK NLN +SHD+A GL+   L  GV +T+V
Sbjct: 79  LFAKMEDTDFVGWALDVLSPNLISTSMLGRVKYNLNSLSHDTATGLIQYALDQGVNVTQV 138

Query: 126 YVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWALEET 185
           +VDTVG PE Y+ +L + FP ++  V  KAD+LYPVVS ASI AKV RD+ ++ W   E 
Sbjct: 139 FVDTVGMPETYQARLQQSFPGIEVTVKAKADALYPVVSAASICAKVARDQAVKKWQFVEK 198

Query: 186 AENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC-TSYFKDIVEVSWESDK 245
            +++  ++GSGYP DP TK WL++H   VFGFP  VRFSW T  T   K+  +V WE   
Sbjct: 199 LQDLDTDYGSGYPNDPKTKAWLKEHVEPVFGFPQFVRFSWRTAQTILEKEAEDVIWE--- 258

Query: 246 VDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLT 296
             +  +    G +++        TS   +E  ++  +   ++F  R LE  T
Sbjct: 259 --DSASENQEGLRKI--------TSYFLNEGSQARPRSSHRYFLERGLESAT 297

BLAST of Csor.00g133450 vs. ExPASy Swiss-Prot
Match: Q2TBT5 (Ribonuclease H2 subunit A OS=Bos taurus OX=9913 GN=RNASEH2A PE=1 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 1.7e-64
Identity = 135/297 (45.45%), Postives = 181/297 (60.94%), Query Frame = 0

Query: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60
           +SS  P P    +PCV+G+DEAGRGPVLGPMVY   YC  S L++L +L  ADSKTL E 
Sbjct: 16  LSSPVP-PVCLKEPCVLGVDEAGRGPVLGPMVYAICYCPLSRLEDLEALKVADSKTLSES 75

Query: 61  KREELFEGLKTN-ESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGV 120
           +R+ LF  ++ + + +GWA+DV+ P  +S+ ML +VK NLN +SHD+A GLV   L  GV
Sbjct: 76  ERDRLFAKMEEDGDFVGWALDVLSPNLISTSMLGRVKYNLNALSHDTATGLVQFALDQGV 135

Query: 121 LLTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDW 180
            + +V+VDTVG PE Y+ +L ++FP ++  V  KAD+LYPVVS ASI AKV RD+ +++W
Sbjct: 136 NVAQVFVDTVGLPETYQERLQQRFPGIEVTVKAKADALYPVVSAASICAKVARDQAVKNW 195

Query: 181 ALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVE-VS 240
              E  +++  ++GSGYP DP TK WL  H   VFGFP  VRFSW T  S  +   E V 
Sbjct: 196 KFVEKLQDLDTDYGSGYPNDPKTKAWLRKHVDPVFGFPQFVRFSWRTAQSILESEAEDVK 255

Query: 241 WESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLT 296
           WE     E G   G GK                SE  ++  +   ++FQ R LE  T
Sbjct: 256 WED---SETGDPKGPGK-----------IKSYFSESPQTCLRLPHRYFQERGLESAT 297

BLAST of Csor.00g133450 vs. NCBI nr
Match: KAG6582048.1 (Ribonuclease H2 subunit A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 599 bits (1545), Expect = 3.46e-216
Identity = 297/297 (100.00%), Postives = 297/297 (100.00%), Query Frame = 0

Query: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60
           MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE
Sbjct: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60

Query: 61  KREELFEGLKTNESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120
           KREELFEGLKTNESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL
Sbjct: 61  KREELFEGLKTNESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180
           LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240

Query: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTQF 297
           SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTQF
Sbjct: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTQF 297

BLAST of Csor.00g133450 vs. NCBI nr
Match: XP_023527419.1 (ribonuclease H2 subunit A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 595 bits (1535), Expect = 1.16e-214
Identity = 295/297 (99.33%), Postives = 295/297 (99.33%), Query Frame = 0

Query: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60
           MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE
Sbjct: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60

Query: 61  KREELFEGLKTNESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120
           KREELFEGLK NESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL
Sbjct: 61  KREELFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180
           LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240

Query: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTQF 297
           SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLT F
Sbjct: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297

BLAST of Csor.00g133450 vs. NCBI nr
Match: XP_022955431.1 (ribonuclease H2 subunit A [Cucurbita moschata])

HSP 1 Score: 595 bits (1535), Expect = 1.16e-214
Identity = 295/297 (99.33%), Postives = 295/297 (99.33%), Query Frame = 0

Query: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60
           MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSK LKEE
Sbjct: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKALKEE 60

Query: 61  KREELFEGLKTNESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120
           KREELFEGLKTNESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL
Sbjct: 61  KREELFEGLKTNESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180
           LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240

Query: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTQF 297
           SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLT F
Sbjct: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297

BLAST of Csor.00g133450 vs. NCBI nr
Match: XP_022979887.1 (ribonuclease H2 subunit A [Cucurbita maxima])

HSP 1 Score: 593 bits (1528), Expect = 1.35e-213
Identity = 293/297 (98.65%), Postives = 295/297 (99.33%), Query Frame = 0

Query: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60
           MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE
Sbjct: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60

Query: 61  KREELFEGLKTNESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120
           KREELFEGLK NESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL
Sbjct: 61  KREELFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180
           LTEVYVDTVGDPEKYRIKLSEKFPNVKFVV+KKADSLYPVVSGASIVAKVTRDRVLRDWA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVSKKADSLYPVVSGASIVAKVTRDRVLRDWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFP+LVRFSWGTCTSYFKDIVEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPSLVRFSWGTCTSYFKDIVEVSWE 240

Query: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTQF 297
           SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLT F
Sbjct: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297

BLAST of Csor.00g133450 vs. NCBI nr
Match: XP_008453982.1 (PREDICTED: ribonuclease H2 subunit A isoform X1 [Cucumis melo] >KAA0044595.1 ribonuclease H2 subunit A isoform X1 [Cucumis melo var. makuwa] >TYK16989.1 ribonuclease H2 subunit A isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 573 bits (1477), Expect = 8.05e-206
Identity = 283/297 (95.29%), Postives = 288/297 (96.97%), Query Frame = 0

Query: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60
           MS QD LPKWASKPCVMGIDEAGRGPVLGPMVYGCLYC  SYLKELSSLNFADSKTLKEE
Sbjct: 1   MSLQDSLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLKELSSLNFADSKTLKEE 60

Query: 61  KREELFEGLKTNESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120
           KREELFE LK NESIGWAVDVIDPRELSSKMLNKVKINLNEISH SAMGLVTKVL MGVL
Sbjct: 61  KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHGSAMGLVTKVLSMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180
           LTEVYVDTVGDPEKYRIKLSEKFPN+KFVVAKKADSLYPVVSGASIVAKVTRDR LR+WA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIVAKVTRDRALREWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKD+VEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDVVEVSWE 240

Query: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTQF 297
           SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQL++F
Sbjct: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLSRF 297

BLAST of Csor.00g133450 vs. ExPASy TrEMBL
Match: A0A6J1GTR5 (Ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111457459 PE=3 SV=1)

HSP 1 Score: 595 bits (1535), Expect = 5.60e-215
Identity = 295/297 (99.33%), Postives = 295/297 (99.33%), Query Frame = 0

Query: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60
           MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSK LKEE
Sbjct: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKALKEE 60

Query: 61  KREELFEGLKTNESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120
           KREELFEGLKTNESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL
Sbjct: 61  KREELFEGLKTNESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180
           LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240

Query: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTQF 297
           SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLT F
Sbjct: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297

BLAST of Csor.00g133450 vs. ExPASy TrEMBL
Match: A0A6J1IPY0 (Ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111479443 PE=3 SV=1)

HSP 1 Score: 593 bits (1528), Expect = 6.54e-214
Identity = 293/297 (98.65%), Postives = 295/297 (99.33%), Query Frame = 0

Query: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60
           MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE
Sbjct: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60

Query: 61  KREELFEGLKTNESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120
           KREELFEGLK NESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL
Sbjct: 61  KREELFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180
           LTEVYVDTVGDPEKYRIKLSEKFPNVKFVV+KKADSLYPVVSGASIVAKVTRDRVLRDWA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVSKKADSLYPVVSGASIVAKVTRDRVLRDWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFP+LVRFSWGTCTSYFKDIVEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPSLVRFSWGTCTSYFKDIVEVSWE 240

Query: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTQF 297
           SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLT F
Sbjct: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297

BLAST of Csor.00g133450 vs. ExPASy TrEMBL
Match: A0A5A7TT92 (Ribonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001310 PE=3 SV=1)

HSP 1 Score: 573 bits (1477), Expect = 3.90e-206
Identity = 283/297 (95.29%), Postives = 288/297 (96.97%), Query Frame = 0

Query: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60
           MS QD LPKWASKPCVMGIDEAGRGPVLGPMVYGCLYC  SYLKELSSLNFADSKTLKEE
Sbjct: 1   MSLQDSLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLKELSSLNFADSKTLKEE 60

Query: 61  KREELFEGLKTNESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120
           KREELFE LK NESIGWAVDVIDPRELSSKMLNKVKINLNEISH SAMGLVTKVL MGVL
Sbjct: 61  KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHGSAMGLVTKVLSMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180
           LTEVYVDTVGDPEKYRIKLSEKFPN+KFVVAKKADSLYPVVSGASIVAKVTRDR LR+WA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIVAKVTRDRALREWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKD+VEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDVVEVSWE 240

Query: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTQF 297
           SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQL++F
Sbjct: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLSRF 297

BLAST of Csor.00g133450 vs. ExPASy TrEMBL
Match: A0A1S3BXP1 (Ribonuclease OS=Cucumis melo OX=3656 GN=LOC103494542 PE=3 SV=1)

HSP 1 Score: 573 bits (1477), Expect = 3.90e-206
Identity = 283/297 (95.29%), Postives = 288/297 (96.97%), Query Frame = 0

Query: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60
           MS QD LPKWASKPCVMGIDEAGRGPVLGPMVYGCLYC  SYLKELSSLNFADSKTLKEE
Sbjct: 1   MSLQDSLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLKELSSLNFADSKTLKEE 60

Query: 61  KREELFEGLKTNESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120
           KREELFE LK NESIGWAVDVIDPRELSSKMLNKVKINLNEISH SAMGLVTKVL MGVL
Sbjct: 61  KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHGSAMGLVTKVLSMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180
           LTEVYVDTVGDPEKYRIKLSEKFPN+KFVVAKKADSLYPVVSGASIVAKVTRDR LR+WA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIVAKVTRDRALREWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKD+VEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDVVEVSWE 240

Query: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTQF 297
           SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQL++F
Sbjct: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLSRF 297

BLAST of Csor.00g133450 vs. ExPASy TrEMBL
Match: A0A0A0KTZ4 (Ribonuclease OS=Cucumis sativus OX=3659 GN=Csa_4G015800 PE=3 SV=1)

HSP 1 Score: 572 bits (1474), Expect = 1.12e-205
Identity = 281/297 (94.61%), Postives = 288/297 (96.97%), Query Frame = 0

Query: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60
           MSSQD LPKW SKPCVMGIDEAGRGPVLGPMVYGCLYC  SYLK+LSSLNFADSKTLKEE
Sbjct: 1   MSSQDSLPKWTSKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLKQLSSLNFADSKTLKEE 60

Query: 61  KREELFEGLKTNESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120
           KREELFE LK NESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVL MGVL
Sbjct: 61  KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLSMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180
           LTEVYVDTVGDPEKYRIKLSEKFPN+KFVVAKKADSLYPVVSGASIVAKVTRDR LR+WA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIVAKVTRDRALREWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKD+VEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDVVEVSWE 240

Query: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTQF 297
           SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIES+ KGRCKFFQARKLEQL++F
Sbjct: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESSGKGRCKFFQARKLEQLSRF 297

BLAST of Csor.00g133450 vs. TAIR 10
Match: AT2G25100.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 446.4 bits (1147), Expect = 1.8e-125
Identity = 212/297 (71.38%), Postives = 257/297 (86.53%), Query Frame = 0

Query: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60
           M S+   P+WAS+PC+MGIDEAGRGPVLGPMVYGC+YC  SY   L+SL+FADSKTLKEE
Sbjct: 1   MESECLTPEWASQPCLMGIDEAGRGPVLGPMVYGCMYCPISYQSSLASLHFADSKTLKEE 60

Query: 61  KREELFEGLKTNESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120
           KREEL+E LK ++S+GWAVDVIDPRELS+KML K K NLNEISH+SAMGL+ +VL MGVL
Sbjct: 61  KREELYESLKLDKSLGWAVDVIDPRELSAKMLAKNKTNLNEISHNSAMGLIKRVLDMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180
           LTE Y+DTVGDP+KYRIKLSE+FP++KFVV+KKADSL+P+VSGASIVAKVTRDR L++W 
Sbjct: 121 LTEAYLDTVGDPDKYRIKLSERFPSIKFVVSKKADSLFPIVSGASIVAKVTRDRALKEWL 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240
           +EET E+++RNFGSGYPGDP TK WL  HKHSVFGFP+LVRFSWGTCT++ K  VEV+WE
Sbjct: 181 VEETGEDINRNFGSGYPGDPETKAWLVQHKHSVFGFPSLVRFSWGTCTTHLKGEVEVAWE 240

Query: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTQF 298
           +D+ +E G  G S K+Q +LS+ G  T +++SEEIES+ KGRCKF QARK++QLTQF
Sbjct: 241 ADENEESG-NGSSSKRQAKLSSFGFKTCEKRSEEIESSGKGRCKFLQARKIQQLTQF 296

BLAST of Csor.00g133450 vs. TAIR 10
Match: AT2G25100.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 410.6 bits (1054), Expect = 1.1e-114
Identity = 199/297 (67.00%), Postives = 241/297 (81.14%), Query Frame = 0

Query: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60
           M S+   P+WAS+PC+MGIDEAGRGPVLGPMVYGC+YC  SY   L+SL+FADSKTLKEE
Sbjct: 1   MESECLTPEWASQPCLMGIDEAGRGPVLGPMVYGCMYCPISYQSSLASLHFADSKTLKEE 60

Query: 61  KREELFEGLKTNESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120
           KREEL+E LK ++S+GWAVDVIDPRELS+KML                    KVL MGVL
Sbjct: 61  KREELYESLKLDKSLGWAVDVIDPRELSAKML-------------------AKVLDMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180
           LTE Y+DTVGDP+KYRIKLSE+FP++KFVV+KKADSL+P+VSGASIVAKVTRDR L++W 
Sbjct: 121 LTEAYLDTVGDPDKYRIKLSERFPSIKFVVSKKADSLFPIVSGASIVAKVTRDRALKEWL 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240
           +EET E+++RNFGSGYPGDP TK WL  HKHSVFGFP+LVRFSWGTCT++ K  VEV+WE
Sbjct: 181 VEETGEDINRNFGSGYPGDPETKAWLVQHKHSVFGFPSLVRFSWGTCTTHLKGEVEVAWE 240

Query: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTQF 298
           +D+ +E G  G S K+Q +LS+ G  T +++SEEIES+ KGRCKF QARK++QLTQF
Sbjct: 241 ADENEESG-NGSSSKRQAKLSSFGFKTCEKRSEEIESSGKGRCKFLQARKIQQLTQF 277

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SEZ62.5e-12471.38Ribonuclease H2 subunit A OS=Arabidopsis thaliana OX=3702 GN=At2g25100 PE=2 SV=2[more]
Q5U2092.3e-6648.98Ribonuclease H2 subunit A OS=Rattus norvegicus OX=10116 GN=Rnaseh2a PE=2 SV=1[more]
Q9CWY85.2e-6649.80Ribonuclease H2 subunit A OS=Mus musculus OX=10090 GN=Rnaseh2a PE=1 SV=2[more]
O757922.6e-6544.52Ribonuclease H2 subunit A OS=Homo sapiens OX=9606 GN=RNASEH2A PE=1 SV=2[more]
Q2TBT51.7e-6445.45Ribonuclease H2 subunit A OS=Bos taurus OX=9913 GN=RNASEH2A PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAG6582048.13.46e-216100.00Ribonuclease H2 subunit A, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023527419.11.16e-21499.33ribonuclease H2 subunit A [Cucurbita pepo subsp. pepo][more]
XP_022955431.11.16e-21499.33ribonuclease H2 subunit A [Cucurbita moschata][more]
XP_022979887.11.35e-21398.65ribonuclease H2 subunit A [Cucurbita maxima][more]
XP_008453982.18.05e-20695.29PREDICTED: ribonuclease H2 subunit A isoform X1 [Cucumis melo] >KAA0044595.1 rib... [more]
Match NameE-valueIdentityDescription
A0A6J1GTR55.60e-21599.33Ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111457459 PE=3 SV=1[more]
A0A6J1IPY06.54e-21498.65Ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111479443 PE=3 SV=1[more]
A0A5A7TT923.90e-20695.29Ribonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001310 ... [more]
A0A1S3BXP13.90e-20695.29Ribonuclease OS=Cucumis melo OX=3656 GN=LOC103494542 PE=3 SV=1[more]
A0A0A0KTZ41.12e-20594.61Ribonuclease OS=Cucumis sativus OX=3659 GN=Csa_4G015800 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G25100.11.8e-12571.38Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT2G25100.21.1e-11467.00Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR024567Ribonuclease HII/HIII domainPFAMPF01351RNase_HIIcoord: 17..227
e-value: 3.8E-52
score: 177.0
IPR023160Ribonuclease HII, helix-loop-helix cap domain superfamilyGENE3D1.10.10.460Ribonuclease hii. Domain 2coord: 187..235
e-value: 6.7E-20
score: 72.5
IPR004649Ribonuclease H2, subunit ATIGRFAMTIGR00729TIGR00729coord: 16..228
e-value: 1.1E-54
score: 183.6
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 2..186
e-value: 6.1E-67
score: 227.2
IPR001352Ribonuclease HII/HIIIPANTHERPTHR10954RIBONUCLEASE H2 SUBUNIT Acoord: 7..297
NoneNo IPR availablePANTHERPTHR10954:SF19RIBONUCLEASEcoord: 7..297
NoneNo IPR availableCDDcd07181RNase_HII_eukaryota_likecoord: 17..233
e-value: 6.37179E-139
score: 389.185
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 15..232

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g133450.m01Csor.00g133450.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043137 DNA replication, removal of RNA primer
biological_process GO:0006298 mismatch repair
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process GO:0016070 RNA metabolic process
cellular_component GO:0032299 ribonuclease H2 complex
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003723 RNA binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity
molecular_function GO:0003676 nucleic acid binding