Csor.00g133110 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g133110
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionrRNA N-glycosidase
LocationCsor_Chr01: 7272088 .. 7272948 (+)
RNA-Seq ExpressionCsor.00g133110
SyntenyCsor.00g133110
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSsinglestart_codonpolypeptidestop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTGCATTGGATCAACTCAAGACTTGATACAAGCTCCCTTTCCCTTAAACTGTGGTGCTCATGCTGCCATTCGTGCTCCTCGTCCTTCCGCCAATGCTAATCTCAGCTTCTCTCTGTTGGGTGCCACTGCCCAAACCTACAAACAATTCATTAATGATCTTCGTAAGGCACTTACGGTAAACTCCCACAAAGTGTACGACATTCCTGTGCTGCCAGCCACGGCCAAAGGGTTAGCACGCTTCATATTGGTCAATCTCACCAATTACAAAGGTGAATCCATCACTGTTGCAATCGACACAGTGGATGTCTATATTGTGGCATACCAAGCAGGAAACAAGGCTTATTTTCTAAAAGATGTTTCCAAAGAAGCCCATGATGTGCTATTTAAAGGTAGCAAACATGAAACGCTGTCGTATAAAGGGAATTATGATGATCTTGAGAGGCATGCAGGTAAGATTTCAAGAGAGAATATTGAGCTTGGATTCTCTGAATTAAGCAGCTCCATTGGCAACATGTTCCACTATAACGCTGGCTATTCTGTGCCTAGATCGTTTATTGTGATGATTCAAATGCTTTCAGAAGCTTCGAGGTTTAAGTATATTGAGACGAGAAATTGTGAGAAGGTTGAATCAAAGTTTAAGCCAGATCCTGGATACTTGAGTTTAGAGAACCGTTGGTCTAATTTATCTGAACATGTACAGAAGGCGCAAAAGCATGGAGGTAAGTTTGAGCGTCCAGTTCAAGTTCGAAGTGTTAGCAATAAACCAATTAAGGTAACTGATGTTAATTCCCCAGTTGTTAAAGGAATTGCGCTGTTACTTTGCAACAAGCCAGGGGTTAATGGAGTATCCAAATGA

mRNA sequence

ATGGAGTGCATTGGATCAACTCAAGACTTGATACAAGCTCCCTTTCCCTTAAACTGTGGTGCTCATGCTGCCATTCGTGCTCCTCGTCCTTCCGCCAATGCTAATCTCAGCTTCTCTCTGTTGGGTGCCACTGCCCAAACCTACAAACAATTCATTAATGATCTTCGTAAGGCACTTACGGTAAACTCCCACAAAGTGTACGACATTCCTGTGCTGCCAGCCACGGCCAAAGGGTTAGCACGCTTCATATTGGTCAATCTCACCAATTACAAAGGTGAATCCATCACTGTTGCAATCGACACAGTGGATGTCTATATTGTGGCATACCAAGCAGGAAACAAGGCTTATTTTCTAAAAGATGTTTCCAAAGAAGCCCATGATGTGCTATTTAAAGGTAGCAAACATGAAACGCTGTCGTATAAAGGGAATTATGATGATCTTGAGAGGCATGCAGGTAAGATTTCAAGAGAGAATATTGAGCTTGGATTCTCTGAATTAAGCAGCTCCATTGGCAACATGTTCCACTATAACGCTGGCTATTCTGTGCCTAGATCGTTTATTGTGATGATTCAAATGCTTTCAGAAGCTTCGAGGTTTAAGTATATTGAGACGAGAAATTGTGAGAAGGTTGAATCAAAGTTTAAGCCAGATCCTGGATACTTGAGTTTAGAGAACCGTTGGTCTAATTTATCTGAACATGTACAGAAGGCGCAAAAGCATGGAGGTAAGTTTGAGCGTCCAGTTCAAGTTCGAAGTGTTAGCAATAAACCAATTAAGGTAACTGATGTTAATTCCCCAGTTGTTAAAGGAATTGCGCTGTTACTTTGCAACAAGCCAGGGGTTAATGGAGTATCCAAATGA

Coding sequence (CDS)

ATGGAGTGCATTGGATCAACTCAAGACTTGATACAAGCTCCCTTTCCCTTAAACTGTGGTGCTCATGCTGCCATTCGTGCTCCTCGTCCTTCCGCCAATGCTAATCTCAGCTTCTCTCTGTTGGGTGCCACTGCCCAAACCTACAAACAATTCATTAATGATCTTCGTAAGGCACTTACGGTAAACTCCCACAAAGTGTACGACATTCCTGTGCTGCCAGCCACGGCCAAAGGGTTAGCACGCTTCATATTGGTCAATCTCACCAATTACAAAGGTGAATCCATCACTGTTGCAATCGACACAGTGGATGTCTATATTGTGGCATACCAAGCAGGAAACAAGGCTTATTTTCTAAAAGATGTTTCCAAAGAAGCCCATGATGTGCTATTTAAAGGTAGCAAACATGAAACGCTGTCGTATAAAGGGAATTATGATGATCTTGAGAGGCATGCAGGTAAGATTTCAAGAGAGAATATTGAGCTTGGATTCTCTGAATTAAGCAGCTCCATTGGCAACATGTTCCACTATAACGCTGGCTATTCTGTGCCTAGATCGTTTATTGTGATGATTCAAATGCTTTCAGAAGCTTCGAGGTTTAAGTATATTGAGACGAGAAATTGTGAGAAGGTTGAATCAAAGTTTAAGCCAGATCCTGGATACTTGAGTTTAGAGAACCGTTGGTCTAATTTATCTGAACATGTACAGAAGGCGCAAAAGCATGGAGGTAAGTTTGAGCGTCCAGTTCAAGTTCGAAGTGTTAGCAATAAACCAATTAAGGTAACTGATGTTAATTCCCCAGTTGTTAAAGGAATTGCGCTGTTACTTTGCAACAAGCCAGGGGTTAATGGAGTATCCAAATGA

Protein sequence

MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEHVQKAQKHGGKFERPVQVRSVSNKPIKVTDVNSPVVKGIALLLCNKPGVNGVSK
Homology
BLAST of Csor.00g133110 vs. ExPASy Swiss-Prot
Match: P98184 (Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 2.0e-83
Identity = 163/259 (62.93%), Postives = 197/259 (76.06%), Query Frame = 0

Query: 17  LNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATA 76
           L  GAH        + + +++FSL+GAT  TYK FI +LR  LTV + +VYDIPVL   A
Sbjct: 12  LYVGAHV-------TEDVDINFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVLRNAA 71

Query: 77  KGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHE 136
            GLARF LV LTNY GES+TVA+D V+VY+VAY+AGN AYFL D S EA++VLF G  H 
Sbjct: 72  AGLARFQLVTLTNYNGESVTVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAGINHV 131

Query: 137 TLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEA 196
            L Y GNYD LE  AG+ISRENIELGFSE+SS+IGNMF +N G SVPR+FIV+IQ +SEA
Sbjct: 132 RLPYGGNYDGLETAAGRISRENIELGFSEISSAIGNMFRHNPGTSVPRAFIVIIQTVSEA 191

Query: 197 SRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEHVQKAQKHGGKFERPVQVRSVSNK 256
           +RFKYIE R  E V +KFKPDP +LSL+N W +LSE +Q AQ  GG+F RPV++R+VSN 
Sbjct: 192 ARFKYIEQRVSENVGTKFKPDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPVELRTVSNT 251

Query: 257 PIKVTDVNSPVVKGIALLL 276
           P  VT+VNSPVVKGIALLL
Sbjct: 252 PTFVTNVNSPVVKGIALLL 263

BLAST of Csor.00g133110 vs. ExPASy Swiss-Prot
Match: P24817 (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN=MAP30 PE=1 SV=2)

HSP 1 Score: 216.9 bits (551), Expect = 3.1e-55
Identity = 120/248 (48.39%), Postives = 168/248 (67.74%), Query Frame = 0

Query: 30  PSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTN 89
           P+A  +++F L  ATA+TY +FI D R  L   SHKVYDIP+L +T     RFIL+NLT+
Sbjct: 19  PTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFILLNLTS 78

Query: 90  YKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLER 149
           Y  E+I+VAID  +VY+VAY+  + +YF K+   EA+++LFKG++  TL Y GNY++L+ 
Sbjct: 79  YAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQT 138

Query: 150 HAGKISRENIELGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEASRFKYIETRNCEK 209
            A KI RENI+LG   LSS+I  +F+YNA  S P + +V+IQ  +EA+RFKYIE    + 
Sbjct: 139 AAHKI-RENIDLGLPALSSAITTLFYYNA-QSAPSALLVLIQTTAEAARFKYIERHVAKY 198

Query: 210 VESKFKPDPGYLSLENRWSNLSEHVQKAQKHGGKFERPVQVRSVSNKPIKVTDVNSPVVK 269
           V + FKP+   +SLEN+WS LS+ +  AQ  GGKF  PV +   + +  +VT+V+S VVK
Sbjct: 199 VATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVK 258

Query: 270 GIALLLCN 278
           G   LL N
Sbjct: 259 GNIKLLLN 263

BLAST of Csor.00g133110 vs. ExPASy Swiss-Prot
Match: P29339 (Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 1.2e-54
Identity = 119/248 (47.98%), Postives = 168/248 (67.74%), Query Frame = 0

Query: 30  PSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTN 89
           P+A  +++F L  ATA+TY +FI D R  L   SHKVYDIP+L +T     RFIL++LT+
Sbjct: 19  PTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFILLDLTS 78

Query: 90  YKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLER 149
           Y  E+I+VAID  +VY+VAY+  + +YF K+   EA+++LFKG++  TL Y GNY++L+ 
Sbjct: 79  YAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQT 138

Query: 150 HAGKISRENIELGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEASRFKYIETRNCEK 209
            A KI RENI+LG   LSS+I  +F+YNA  S P + +V+IQ  +EA+RFKYIE    + 
Sbjct: 139 AAHKI-RENIDLGLPALSSAITTLFYYNA-QSAPSALLVLIQTTAEAARFKYIERHVAKY 198

Query: 210 VESKFKPDPGYLSLENRWSNLSEHVQKAQKHGGKFERPVQVRSVSNKPIKVTDVNSPVVK 269
           V + FKP+   +SLEN+WS LS+ +  AQ  GGKF  PV +   + +  +VT+V+S VVK
Sbjct: 199 VATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVK 258

Query: 270 GIALLLCN 278
           G   LL N
Sbjct: 259 GNIKLLLN 263

BLAST of Csor.00g133110 vs. ExPASy Swiss-Prot
Match: P84531 (Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2)

HSP 1 Score: 202.6 bits (514), Expect = 6.0e-51
Identity = 115/242 (47.52%), Postives = 158/242 (65.29%), Query Frame = 0

Query: 35  NLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGES 94
           N+ F L  AT+ +YK FI +LR+AL  +  KVYDIPVL +T     RFIL++L NY G+S
Sbjct: 1   NVRFDLSSATSSSYKTFIKNLREALPKDG-KVYDIPVLLSTVMDSRRFILIDLVNYDGQS 60

Query: 95  ITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKI 154
           IT AID ++VYIVAY  G  +YF + V  +A  +LFKG++  TL Y GNY++L+  A K+
Sbjct: 61  ITAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKL 120

Query: 155 SRENIELGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKF 214
            RENIELG   L S+I  +FHYNA  +   + +V+IQ  SEA+RF+YIE +    V +KF
Sbjct: 121 -RENIELGLPALDSAITTLFHYNA-EAAASALLVLIQTTSEAARFRYIELQIANNVGTKF 180

Query: 215 KPDPGYLSLENRWSNLSEHVQKAQKHGGKFERPVQVRSVSNKPIKVTDVNSPVV-KGIAL 274
           KP    +SLEN WS LS+ +Q A+   G+FE PV +       +++T+V S VV + I L
Sbjct: 181 KPSQTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQITNVTSNVVTQNIQL 239

Query: 275 LL 276
           LL
Sbjct: 241 LL 239

BLAST of Csor.00g133110 vs. ExPASy Swiss-Prot
Match: P09989 (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 190.7 bits (483), Expect = 2.4e-47
Identity = 109/251 (43.43%), Postives = 163/251 (64.94%), Query Frame = 0

Query: 30  PSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTN 89
           P+   ++SF L GAT+ +Y  FI++LRKAL  N  K+YDIP+L ++  G  R+ L++LTN
Sbjct: 19  PAVEGDVSFRLSGATSSSYGVFISNLRKALP-NERKLYDIPLLRSSLPGSQRYALIHLTN 78

Query: 90  YKGESITVAIDTVDVYIVAYQAGNKAYFLKDVS-KEAHDVLFKGSKHE-TLSYKGNYDDL 149
           Y  E+I+VAID  +VYI+ Y+AG+ +YF  + S  EA   +FK +  + TL Y GNY+ L
Sbjct: 79  YADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFKDAMRKVTLPYSGNYERL 138

Query: 150 ERHAGKISRENIELGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEASRFKYIETRNC 209
           +  AGKI RENI LG   L S+I  +F+YNA  S   + +V+IQ  SEA+R+K+IE +  
Sbjct: 139 QTAAGKI-RENIPLGLPALDSAITTLFYYNAN-SAASALMVLIQSTSEAARYKFIEQQIG 198

Query: 210 EKVESKFKPDPGYLSLENRWSNLSEHVQKAQKHGGKFERPVQVRSVSNKPIKVTDVNSPV 269
           ++V+  F P    +SLEN WS LS+ +Q A  + G+FE PV + +  N+ + +T+V++ V
Sbjct: 199 KRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINAQNQRVTITNVDAGV 258

Query: 270 VKGIALLLCNK 279
           V     LL N+
Sbjct: 259 VTSNIALLLNR 266

BLAST of Csor.00g133110 vs. NCBI nr
Match: KAG6607631.1 (hypothetical protein SDJN03_00973, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 574 bits (1479), Expect = 1.70e-206
Identity = 286/286 (100.00%), Postives = 286/286 (100.00%), Query Frame = 0

Query: 1   MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALT 60
           MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALT
Sbjct: 1   MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALT 60

Query: 61  VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD 120
           VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD
Sbjct: 61  VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD 120

Query: 121 VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYNAGY 180
           VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYNAGY
Sbjct: 121 VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYNAGY 180

Query: 181 SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEHVQKAQKH 240
           SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEHVQKAQKH
Sbjct: 181 SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEHVQKAQKH 240

Query: 241 GGKFERPVQVRSVSNKPIKVTDVNSPVVKGIALLLCNKPGVNGVSK 286
           GGKFERPVQVRSVSNKPIKVTDVNSPVVKGIALLLCNKPGVNGVSK
Sbjct: 241 GGKFERPVQVRSVSNKPIKVTDVNSPVVKGIALLLCNKPGVNGVSK 286

BLAST of Csor.00g133110 vs. NCBI nr
Match: KAG7037232.1 (hypothetical protein SDJN02_00855, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 564 bits (1454), Expect = 2.22e-202
Identity = 281/286 (98.25%), Postives = 283/286 (98.95%), Query Frame = 0

Query: 1   MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALT 60
           MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALT
Sbjct: 1   MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALT 60

Query: 61  VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD 120
           VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD
Sbjct: 61  VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD 120

Query: 121 VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYNAGY 180
           VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYNAGY
Sbjct: 121 VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYNAGY 180

Query: 181 SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEHVQKAQKH 240
           SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSE VQKAQKH
Sbjct: 181 SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKH 240

Query: 241 GGKFERPVQVRSVSNKPIKVTDVNSPVVKGIALLLCNKPGVNGVSK 286
           GGKFE PVQVRSVSNKP+ V+DVNSPVVKGIALLLCNKPGVNGVSK
Sbjct: 241 GGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALLLCNKPGVNGVSK 286

BLAST of Csor.00g133110 vs. NCBI nr
Match: XP_023523582.1 (ribosome-inactivating protein bryodin II-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 561 bits (1447), Expect = 2.59e-201
Identity = 281/286 (98.25%), Postives = 281/286 (98.25%), Query Frame = 0

Query: 1   MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALT 60
           MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLR ALT
Sbjct: 1   MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRNALT 60

Query: 61  VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD 120
           V SHKVY IPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD
Sbjct: 61  VQSHKVYGIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD 120

Query: 121 VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYNAGY 180
           VSKEAHDVLFKGSKHETLSYKGNYDDLERHAG ISRENIELGFSELSSSIGNMFHYNAGY
Sbjct: 121 VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGMISRENIELGFSELSSSIGNMFHYNAGY 180

Query: 181 SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEHVQKAQKH 240
           SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSE VQKAQKH
Sbjct: 181 SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKH 240

Query: 241 GGKFERPVQVRSVSNKPIKVTDVNSPVVKGIALLLCNKPGVNGVSK 286
           GGKFERPVQVRSVSNKPIKVTDVNSPVVKGIALLLCNKPGVNGVSK
Sbjct: 241 GGKFERPVQVRSVSNKPIKVTDVNSPVVKGIALLLCNKPGVNGVSK 286

BLAST of Csor.00g133110 vs. NCBI nr
Match: XP_022926255.1 (ribosome-inactivating protein bryodin II-like [Cucurbita moschata])

HSP 1 Score: 558 bits (1438), Expect = 6.10e-200
Identity = 279/286 (97.55%), Postives = 281/286 (98.25%), Query Frame = 0

Query: 1   MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALT 60
           MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALT
Sbjct: 1   MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALT 60

Query: 61  VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD 120
           VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD
Sbjct: 61  VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD 120

Query: 121 VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYNAGY 180
           VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHY AGY
Sbjct: 121 VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYKAGY 180

Query: 181 SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEHVQKAQKH 240
           SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSE VQKAQKH
Sbjct: 181 SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKH 240

Query: 241 GGKFERPVQVRSVSNKPIKVTDVNSPVVKGIALLLCNKPGVNGVSK 286
           GGKFE PVQVRSVSNKP+ V+DVNSPVVKGIALLL NKPGVNGVSK
Sbjct: 241 GGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALLLSNKPGVNGVSK 286

BLAST of Csor.00g133110 vs. NCBI nr
Match: XP_022981562.1 (ribosome-inactivating protein bryodin II-like [Cucurbita maxima])

HSP 1 Score: 552 bits (1422), Expect = 1.68e-197
Identity = 273/286 (95.45%), Postives = 280/286 (97.90%), Query Frame = 0

Query: 1   MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALT 60
           MECIGST+DLIQAPFPLNCGAH AIRAPRPSANANLSFSLLGATAQTYKQFI+DLRKALT
Sbjct: 1   MECIGSTKDLIQAPFPLNCGAHVAIRAPRPSANANLSFSLLGATAQTYKQFISDLRKALT 60

Query: 61  VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD 120
           VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD
Sbjct: 61  VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD 120

Query: 121 VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYNAGY 180
           VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENI+LGFSELSSSIGNMFHYNAGY
Sbjct: 121 VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIDLGFSELSSSIGNMFHYNAGY 180

Query: 181 SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEHVQKAQKH 240
           SVP+SFIVMIQMLSEASRFKYIE RNCE VESKFKPDPGYLSLENRWSNLSE VQKAQKH
Sbjct: 181 SVPKSFIVMIQMLSEASRFKYIEARNCENVESKFKPDPGYLSLENRWSNLSEQVQKAQKH 240

Query: 241 GGKFERPVQVRSVSNKPIKVTDVNSPVVKGIALLLCNKPGVNGVSK 286
           GGKFE PVQVRSVSNKP+ V+DVNSPVVKG+ALLLCNKPGVNGVSK
Sbjct: 241 GGKFEHPVQVRSVSNKPVLVSDVNSPVVKGMALLLCNKPGVNGVSK 286

BLAST of Csor.00g133110 vs. ExPASy TrEMBL
Match: A0A6J1EKL0 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111433434 PE=3 SV=1)

HSP 1 Score: 558 bits (1438), Expect = 2.95e-200
Identity = 279/286 (97.55%), Postives = 281/286 (98.25%), Query Frame = 0

Query: 1   MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALT 60
           MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALT
Sbjct: 1   MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALT 60

Query: 61  VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD 120
           VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD
Sbjct: 61  VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD 120

Query: 121 VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYNAGY 180
           VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHY AGY
Sbjct: 121 VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYKAGY 180

Query: 181 SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEHVQKAQKH 240
           SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSE VQKAQKH
Sbjct: 181 SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKH 240

Query: 241 GGKFERPVQVRSVSNKPIKVTDVNSPVVKGIALLLCNKPGVNGVSK 286
           GGKFE PVQVRSVSNKP+ V+DVNSPVVKGIALLL NKPGVNGVSK
Sbjct: 241 GGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALLLSNKPGVNGVSK 286

BLAST of Csor.00g133110 vs. ExPASy TrEMBL
Match: A0A6J1IUB2 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1)

HSP 1 Score: 552 bits (1422), Expect = 8.11e-198
Identity = 273/286 (95.45%), Postives = 280/286 (97.90%), Query Frame = 0

Query: 1   MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALT 60
           MECIGST+DLIQAPFPLNCGAH AIRAPRPSANANLSFSLLGATAQTYKQFI+DLRKALT
Sbjct: 1   MECIGSTKDLIQAPFPLNCGAHVAIRAPRPSANANLSFSLLGATAQTYKQFISDLRKALT 60

Query: 61  VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD 120
           VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD
Sbjct: 61  VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD 120

Query: 121 VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYNAGY 180
           VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENI+LGFSELSSSIGNMFHYNAGY
Sbjct: 121 VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIDLGFSELSSSIGNMFHYNAGY 180

Query: 181 SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEHVQKAQKH 240
           SVP+SFIVMIQMLSEASRFKYIE RNCE VESKFKPDPGYLSLENRWSNLSE VQKAQKH
Sbjct: 181 SVPKSFIVMIQMLSEASRFKYIEARNCENVESKFKPDPGYLSLENRWSNLSEQVQKAQKH 240

Query: 241 GGKFERPVQVRSVSNKPIKVTDVNSPVVKGIALLLCNKPGVNGVSK 286
           GGKFE PVQVRSVSNKP+ V+DVNSPVVKG+ALLLCNKPGVNGVSK
Sbjct: 241 GGKFEHPVQVRSVSNKPVLVSDVNSPVVKGMALLLCNKPGVNGVSK 286

BLAST of Csor.00g133110 vs. ExPASy TrEMBL
Match: A0A6J1E093 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1)

HSP 1 Score: 374 bits (959), Expect = 2.78e-127
Identity = 198/289 (68.51%), Postives = 231/289 (79.93%), Query Frame = 0

Query: 1   MECIGSTQDLIQAPFPLNC----GAHA--AIRAPRPSANANLSFSLLGATAQTYKQFIND 60
           MEC+ S++  IQ P P N     G HA  A+ AP    N+N+SFSLLGAT +TY+QFI +
Sbjct: 1   MECVRSSEPFIQ-PLPFNDIGIHGVHANGAVNAP----NSNVSFSLLGATGKTYQQFIQN 60

Query: 61  LRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNK 120
           LR ALT+NS  VY+IPVL ATA   ARFILV+LTNYK E+ITVAID V+VYIVAYQAGNK
Sbjct: 61  LRNALTINSKIVYNIPVLAATALSSARFILVHLTNYKNETITVAIDVVNVYIVAYQAGNK 120

Query: 121 AYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMF 180
           AYFLKD SKEA DVLFKG K E L YKGNYD LE  AGKISRE I+LGFSEL S+IGNM+
Sbjct: 121 AYFLKDASKEARDVLFKGIKQEILPYKGNYDGLETAAGKISREKIDLGFSELGSAIGNMY 180

Query: 181 HYNAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEHV 240
           HYNAG SVPR+FIVMIQ +SEA+RFKYIET+  + VE+KFKPDPG+LSLENRWS+LSE V
Sbjct: 181 HYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQNVETKFKPDPGFLSLENRWSDLSEQV 240

Query: 241 QKAQKHGGKFERPVQVRSVSNKPIKVTDVNSPVVKGIALLLCNKPGVNG 283
           Q AQ   G+F RP++VRSV+NKPI VT+V S VV+G+ALLL +KP  NG
Sbjct: 241 QIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEGMALLLYSKPRGNG 284

BLAST of Csor.00g133110 vs. ExPASy TrEMBL
Match: A0A6J1DVE7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1)

HSP 1 Score: 333 bits (854), Expect = 9.86e-112
Identity = 172/251 (68.53%), Postives = 205/251 (81.67%), Query Frame = 0

Query: 31  SANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNY 90
           +A A++SFS+LG+T++TYKQFI ++R ALTV S  VYDIPVL ATA G ARF+LV+LTNY
Sbjct: 18  AAEADVSFSMLGSTSETYKQFIKNVRSALTVGSQIVYDIPVLKATATGSARFLLVHLTNY 77

Query: 91  KGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERH 150
             ESI VAID V+VY+VAY+AGN AYFL D S EA  VLF+G  H  LSYKGNYD LE  
Sbjct: 78  NSESIIVAIDVVNVYVVAYRAGNNAYFLNDASAEADRVLFQGINHVKLSYKGNYDGLET- 137

Query: 151 AGKISRENIELGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKV 210
           A KISRE I+LGFSE+SSSIGNMFH+NAG SVPR+FIV+IQ +SEA+RFKYIE R  E V
Sbjct: 138 AAKISREKIDLGFSEISSSIGNMFHHNAGTSVPRAFIVIIQSVSEAARFKYIEQRVSENV 197

Query: 211 ESKFKPDPGYLSLENRWSNLSEHVQKAQKHGGKFERPVQVRSVSNKPIKVTDVNSPVVKG 270
           ++ FKPDP +LSL+NRWS+LSE +Q AQK GG+F R V++R+VSNKPI VTDV+SPVVKG
Sbjct: 198 KTTFKPDPAFLSLQNRWSDLSEQIQIAQKRGGEFARAVELRTVSNKPILVTDVSSPVVKG 257

Query: 271 IALLLCNKPGV 281
           IALLL  K  V
Sbjct: 258 IALLLYYKVSV 267

BLAST of Csor.00g133110 vs. ExPASy TrEMBL
Match: A0A6J1DWK4 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1)

HSP 1 Score: 328 bits (842), Expect = 6.55e-110
Identity = 165/248 (66.53%), Postives = 203/248 (81.85%), Query Frame = 0

Query: 31  SANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNY 90
           +A A++SFS+LGAT++TY+QFI +LR  LT+ S  VY IPVL +TA G ARFILV+LTNY
Sbjct: 18  AAEADVSFSMLGATSETYRQFIRNLRSTLTIRSPVVYGIPVLQSTAVGSARFILVHLTNY 77

Query: 91  KGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERH 150
             ESITVAID V++Y+VAY+AGN AYFL D S EA++VLFKG KH  L YKGNYD LE  
Sbjct: 78  NTESITVAIDVVNIYVVAYRAGNNAYFLNDASAEANNVLFKGIKHVRLPYKGNYDGLETA 137

Query: 151 AGKISRENIELGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKV 210
           AGKISRE I+LGFSE+SSSIGNMFH+N G S+ R+FIV+IQ + EA+RFKYIE R  E V
Sbjct: 138 AGKISREKIDLGFSEISSSIGNMFHHNVGTSIARAFIVIIQSVLEAARFKYIEQRVSENV 197

Query: 211 ESKFKPDPGYLSLENRWSNLSEHVQKAQKHGGKFERPVQVRSVSNKPIKVTDVNSPVVKG 270
           ++KFKPDP +LSLENRWS+LSE +Q AQK  G+F R +++R+VSNKPI VT+V+SP+VKG
Sbjct: 198 KTKFKPDPAFLSLENRWSDLSEQIQIAQKREGEFARAIELRTVSNKPIIVTNVSSPIVKG 257

Query: 271 IALLLCNK 278
           IALLL  K
Sbjct: 258 IALLLYYK 265

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P981842.0e-8362.93Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1[more]
P248173.1e-5548.39Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN... [more]
P293391.2e-5447.98Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV... [more]
P845316.0e-5147.52Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2[more]
P099892.4e-4743.43Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=... [more]
Match NameE-valueIdentityDescription
KAG6607631.11.70e-206100.00hypothetical protein SDJN03_00973, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7037232.12.22e-20298.25hypothetical protein SDJN02_00855, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_023523582.12.59e-20198.25ribosome-inactivating protein bryodin II-like [Cucurbita pepo subsp. pepo][more]
XP_022926255.16.10e-20097.55ribosome-inactivating protein bryodin II-like [Cucurbita moschata][more]
XP_022981562.11.68e-19795.45ribosome-inactivating protein bryodin II-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1EKL02.95e-20097.55rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111433434 PE=3 SV=1[more]
A0A6J1IUB28.11e-19895.45rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1[more]
A0A6J1E0932.78e-12768.51rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1[more]
A0A6J1DVE79.86e-11268.53rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1[more]
A0A6J1DWK46.55e-11066.53rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017989Ribosome-inactivating protein type 1/2PRINTSPR00396SHIGARICINcoord: 94..109
score: 44.9
coord: 218..235
score: 41.96
coord: 156..170
score: 48.77
coord: 46..59
score: 42.29
coord: 186..206
score: 53.88
IPR016139Ribosome-inactivating protein, subdomain 2GENE3D4.10.470.10Ricin (A Subunit), domain 2coord: 199..281
e-value: 1.8E-23
score: 84.1
IPR001574Ribosome-inactivating proteinPFAMPF00161RIPcoord: 44..235
e-value: 2.5E-34
score: 118.6
IPR001574Ribosome-inactivating proteinPANTHERPTHR33453FAMILY NOT NAMEDcoord: 36..275
IPR016138Ribosome-inactivating protein, subdomain 1GENE3D3.40.420.10Ricin (A subunit), domain 1coord: 35..198
e-value: 1.4E-45
score: 157.0
NoneNo IPR availablePANTHERPTHR33453:SF14RRNA N-GLYCOSIDASEcoord: 36..275
IPR036041Ribosome-inactivating protein superfamilySUPERFAMILY56371Ribosome inactivating proteins (RIP)coord: 36..276

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g133110.m01Csor.00g133110.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
biological_process GO:0035821 modulation of process of other organism
biological_process GO:0017148 negative regulation of translation
molecular_function GO:0030598 rRNA N-glycosylase activity
molecular_function GO:0090729 toxin activity