Csor.00g127540 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g127540
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionE3 ubiquitin-protein ligase Praja-2
LocationCsor_Chr07: 6824548 .. 6829317 (-)
RNA-Seq ExpressionCsor.00g127540
SyntenyCsor.00g127540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGGAGGTTTGAATCACTGGAAGCGAACTTTGATCTTCGTCTGTTTCGTTTTATTATGCAGTTTTTCGTTTTCCCTAATTGTATGTAATGTCATTTTTGTGCTCTCTGTGCTAATCATTGGGTGATTGATCTGTTTTCTTCTGTTGCGATTGCGTTGAAATTGAAAACCCTTAGTTTGGACGAAATTATTTCCCTTTTTGTCTTGTTTGCTGATTGAACTTCTGGAACCACTCTTGAACCCTTCAAATACATGTTAGTGGGATGGAGTTTGTGTTGTTGGAGGGTTTTAGCATCAAAATTGGGGATGGATGTGTGAGATCCCGCATCGGTTGGGGAGGAGAACAAAACATTCTTCATAAGGGTGTGGAAACCTCTCCCTAGCAGACGCGTTTTAAAAACTTTGAGGGGGGAAGCCTAGAAGGGGAAGCCTAGAAGGGAAAGCCTAAAGAGGACAATATGTGCTAGTGGTGTGCTTAGCTGCTACAGGACCTTGACGCGTTTGAGAATATTGTTCCCTCGGTTCTACTTTTGAAGTTCTAGGAGTTTGAACTCCTGGTGGGGCAATGGAGGGAGGTTGGAAAGGGCAGTTCATTTGGATTGAGATAAGAAAATGGTTTAGTTTGAAACAAAGAAGTGAAGTGAGGTTAGGAATGAAGTGGTAAAGGAGATATCTAATTCTGCTTATGTGATGTTCTTCTCTCTTTTAAATCTCCTCTCAACTAGGGGTTGTGAAGGGTTACGAGTAGTTGTTAATGTACAAGAGGAAACACGGACGAGGAGTGGCTCGTGACAGGTTTCATGGTGAATGAAGCGGTATCAATTGAGAATGAGAGTAAGTTTGAGATAGTACAAATATAAAACAACTTAGTTAAGAGCTTGAATACATGGCTACAAGGCTAGAAAACTATGACAATTCAGTGTGGTCGGTCTCTCAAGTCGTTTGATCATCTCTGTATAACTGGCATTGTTTTTCATATTTGATTGTGTTGAGGATTGTTCGGAGGGAGTCCCACATTGAATAATTTAGAGGATGATCATGGGTTTATAAGTAAAGAATAAATCTCCATTCGTATGAGGCCTTTTGGGGAAACCCAAAGAAAAACCGTGAGAGCTTATGCTCAAAGTGGATAATATCATACCATTGTGGAGGTCCGGAGGTCCGTGATTCCTAACAGACTGAACTTCAACTTAGTTAAATATGATTCTTATTTCACTACTTGTTGAACTTCATGTTGGTTTTCCACTGTTTGTGATTTTTGCTTGCTGGTCTCGTCTCAGATGGACATCGACTTACAAAGAATCTTGGAGGAGTTTCTTCAATCCACTGCTCATGTGATGGACAATAATGAACTGGTTGATGAACATGGTCTGAATCAATCGCCAACTTGTGCATGTTGCGAACGATTCCTAGTACTTGATAACGATGTCAGTGGTGAACCTGAAGCCATTGGTATATGTGGCGATTGTAAGTTTTTGTTACTTGAAGATATGGAATTCCGTGAACAGGATTCTTATAACAGGGCGATGCCAAGAGGAAGAAGAACAAGGCATGGTAGTTCAGAGTCTCCTGAAAGTCGTTTTTCGCAGGAATTCTCCCATATGATTAACTTGGTGAGGCAAAGTCAGTCGAGTATCTCTAGCTACGGGGATCGGTATGCAGATGGTTCTAATGCTACTTATGCTACGCAGGACTCGAGTTTGCTTATGAGCCCCAATCAATCTAGAAGATGGCGTCGAGCAAACTCTGATTCTGAAAATGATGGTTTAGATAGTATTGACTCTATGTTTCGAGAGAGTGGATCAAATTTCAGTTTTGGGTCTTACAGACATTCTTATGGTGATATTGATTTTGTTTCGTACGGTACTTATGGGGGTGACTCGGACGCTTCCATGGATGGACGAAGTTCCTTGGACACAGATGTTTTCGTTTTTCCTGGTGATGGATTCAATGATACCGATATAGACATTGATCCTATGCGTGCTGGTGTCGGCGATTGGGACTCGAGTGATGAGGAGGAGGATCTTGGTGAATTAACAGAAGCTGACACAGAGCAAGATGCACTCGAAGTAGAATCTTCTCAAGCTAGGCCTCCACTTCAAGATTTTCAAGTTTCTAGTTCTTCTGGGAGAGTTAACTCTGGTAATTGGAATGAACAACTTTCTTCACCAGAATACGAACTCGGGCGAATCAGGAGGAATGGGCGATATTATTCGAACATGAACTATTCACAGTCGGAATTACTATCTTTCGTTGAGAATTTTGGTGAATATCTTGACCAACAAGGCTTTGAACAACTTCTTGAGCAAATTGCCATGACGACCAGCTCAAGACGCGGAGCACCTCCTGCAGCTGTCTCTTCAGTGAAAAATCTCCCACTTTTGGTGATTTCTAAAGAACATTTGAAACATGACAATATATCCTGTGCAATTTGTAAGGATTTCTTACTCGTGGGCGTCGAAGTGAATCAACTACCTTGCCTTCATCTCTACCACCCTTCCTGCATCATGCCGTGGTTGCGTACACGGAATTCGTGCCCCCTTTGTCGATATGAACTTCCAACCGACGATCGAGATTACGAAGAGGGAAAGCAAAGTAGCATCATTACAGCAGAGGTCCAAGGAGTTGGAGCAAATCTAGAGTTTAGAACCAGCCAAGGAGAGGAGCTGAACAACATAGACTCTGCAGTTAATAACAGTAACAATTCAGGCAGACACGACGGTATAAGAAGTTGGGTGTTCGTGGCTGCTCCGATCGTTGGTCTTGCAGGTATAGCCCTGATGTTATGGTTTGGTAGCCCTCGGTGTGATCAAAGAGTACCCGTAGGCCAAGTTGCAGACAGAAGGCAACTTGATGCTCACATTGCTGATGCATTGAACCAAAGAGAGAACAGAAGCAGGAGATGGTGGTGAATTGTCTAGCAATAGCGTATGGTACAAGAAATCAAATTAACACGAGCTTTCTTTTACTTCAATGATAATGCTTATATGAAGTTCATACACAATTGTTTATATCAAAATGTTCTTCACATGAATGGGCATTTAGGTATCCAAATGTTTATCTCCACATGAATATTCTGCAGCGTTCGAGTATCCTTGGAGCGGCGAACGATGGTGGTTTTCTTCAGCATTTAGTCTATTGTTTTCGGCCAATATCCGATGGAGAAGGTAAAATCAAATTTCCAAACCTTGAAGTTTATACGAGTTTATTTCCAAAGTTCAATATATTCGAGGATATTATGATTTTAGTCATGTACTGTCCTGTTCTTTTGTCTGTTTCGACGTTGGTTTGTTTTGTTTATGAGAGTTGGTTTTCCATTTTTACATTTATGTTCTGTTCCATGATGTTTGAAGTCCCTAAGACTACTATGTTTTGGTTTATTCTAAGAAGCTTGTTAACTTTGTCGTGATTTAGATTTAGACCAATACTAAGGTTAGCTTGTTGTCTGCTCAACAATTCTCAACGTAACTCAACGTAAGCACAAGGTTTAGTTTGGACGAGAACTTTGAGTATCGTGTGAAGATAATTCAATGACTAGAATGTGAAGTTCACATAAAACTGGAATTTAGATTGAAACTTCTAACATCGTTTAGCTTGAGATCAACCGAACAAGAACATAGGGTAGTCAACACTTATATCCAAACGATGTAGTTGACTCCTTTTTTGATAAGCCAAGAAATGGGTTATGTCGTAAGATTTAGCATTAGATTAGCATTTTCATATACCTTTTAACCAATGACTATACATGATTCCATAAATGAATCAATGGCACGAAATTAAAGGTTATGGTAAAACTTCGTTTAAAACGATGTGGTAGAAAGTAACGTGCGACTTGAAGGACATGATCTACTGTGGATTCTTATACCCACCATTTTATATTATATAGGAATGAAGATGCTCTTGACTCGAAATCGTTTGTTCTCTTCCAATAGAGCTCTAATTTTTTGAGGATTTTGATGTAAATAGTACACGATGGAGCTCGAGTAGACAGTATTGATTCATTTTTCAAGGGCAGAGATCTAGGGTTTGTGTCAATCAATAAGTTAAAACTACTTCGTAAGGTATATGTTCGGTATAGAAATCGAAAGGATCCAATTCGACTAGAGATGATACATTTTGAATGAGGTTTAGTCCATGGTCATTATGTTTCTAGCTCTCCGACTATTCTAAATAAATCTCATTAGCTTTTCTATCTTCATATATTGTGATAAGTTTAGTTGAAATTTCAAATGCATCGATTGATGTAAATGAGCAAGGGCTTGGAGGCATGTATTGTGTCGTATTGTTTGTCAAGTAAGGATCTTGCTCACTGATGCGGATGCGACCGATAAATTGCTATGAAGTATCATAACAAATTTCTCTCTTTATATTGCACACATCATTTGTTGTATTTGCTACCATGCTTTATGTTTTTGGGCAAGACAATACAGAGCTGTTCTTAGCTGCGACAAAGTAATTGTATCCATCCCTTAGTTGCATCACCTAATTCTTGCTTTGATCCCCAAGTTTAAAACCAAAAATCACTCGTGTTCATCGACGTGCTTTCATCAAATTTCGGTCATCTAACTCTTAGTTTGAAGTTTCAGTTCAATTTTTATGTAAGTTGCTTTGAATAACCTTTTTAGACTTATCAAGATTTGTTAGTTAGTCGCTTGTCTGATGGCAGGAATCATCGATTACATAATTGGAATTTGGCGTTCTCCTTTGGGTGGAAAATCCGAAAAGCTCAATTTTTTTGTCAAGTTATTTCTTGTAGTCAAGTAGTCTAG

mRNA sequence

ATGTTGGAGATGGACATCGACTTACAAAGAATCTTGGAGGAGTTTCTTCAATCCACTGCTCATGTGATGGACAATAATGAACTGGTTGATGAACATGGTCTGAATCAATCGCCAACTTGTGCATGTTGCGAACGATTCCTAGTACTTGATAACGATGTCAGTGGTGAACCTGAAGCCATTGGTATATGTGGCGATTGTAAGTTTTTGTTACTTGAAGATATGGAATTCCGTGAACAGGATTCTTATAACAGGGCGATGCCAAGAGGAAGAAGAACAAGGCATGGTAGTTCAGAGTCTCCTGAAAGTCGTTTTTCGCAGGAATTCTCCCATATGATTAACTTGGACTCGAGTTTGCTTATGAGCCCCAATCAATCTAGAAGATGGCGTCGAGCAAACTCTGATTCTGAAAATGATGGTTTAGATAGTATTGACTCTATGTTTCGAGAGAGTGGATCAAATTTCAGTTTTGGGTCTTACAGACATTCTTATGGTGATATTGATTTTGTTTCGTACGGTACTTATGGGGGTGACTCGGACGCTTCCATGGATGGACGAAGTTCCTTGGACACAGATGTTTTCGTTTTTCCTGGTGATGGATTCAATGATACCGATATAGACATTGATCCTATGCGTGCTGGTGTCGGCGATTGGGACTCGAGTGATGAGGAGGAGGATCTTGGTGAATTAACAGAAGCTGACACAGAGCAAGATGCACTCGAAGTAGAATCTTCTCAAGCTAGGCCTCCACTTCAAGATTTTCAAGTTTCTAGTTCTTCTGGGAGAGTTAACTCTGGTAATTGGAATGAACAACTTTCTTCACCAGAATACGAACTCGGGCGAATCAGGAGGAATGGGCGATATTATTCGAACATGAACTATTCACAGTCGGAATTACTATCTTTCGTTGAGAATTTTGGTGAATATCTTGACCAACAAGGCTTTGAACAACTTCTTGAGCAAATTGCCATGACGACCAGCTCAAGACGCGGAGCACCTCCTGCAGCTGTCTCTTCAGTGAAAAATCTCCCACTTTTGGTGATTTCTAAAGAACATTTGAAACATGACAATATATCCTGTGCAATTTGTAAGGATTTCTTACTCGTGGGCGTCGAAGTGAATCAACTACCTTGCCTTCATCTCTACCACCCTTCCTGCATCATGCCGTGGTTGCGTACACGGAATTCGTGCCCCCTTTGTCGATATGAACTTCCAACCGACGATCGAGATTACGAAGAGGGAAAGCAAAGTAGCATCATTACAGCAGAGGTCCAAGGAGTTGGAGCAAATCTAGAGTTTAGAACCAGCCAAGGAGAGGAGCTGAACAACATAGACTCTGCAGTTAATAACAGTAACAATTCAGGCAGACACGACGGTATAAGAAGTTGGGTGTTCGTGGCTGCTCCGATCGTTGGTCTTGCAGGTATAGCCCTGATGTTATGGTTTGGTAGCCCTCGGTGTGATCAAAGAAGAGAACAGAAGCAGGAGATGGTGGTGAATTGTCTAGCAATAGCGTATGCGTTCGAGTATCCTTGGAGCGGCGAACGATGGTGGTTTTCTTCAGCATTTAGTCTATTGTTTTCGGCCAATATCCGATGGAGAAGGAATCATCGATTACATAATTGGAATTTGGCGTTCTCCTTTGGGTGGAAAATCCGAAAAGCTCAATTTTTTTGTCAAGTTATTTCTTGTAGTCAAGTAGTCTAG

Coding sequence (CDS)

ATGTTGGAGATGGACATCGACTTACAAAGAATCTTGGAGGAGTTTCTTCAATCCACTGCTCATGTGATGGACAATAATGAACTGGTTGATGAACATGGTCTGAATCAATCGCCAACTTGTGCATGTTGCGAACGATTCCTAGTACTTGATAACGATGTCAGTGGTGAACCTGAAGCCATTGGTATATGTGGCGATTGTAAGTTTTTGTTACTTGAAGATATGGAATTCCGTGAACAGGATTCTTATAACAGGGCGATGCCAAGAGGAAGAAGAACAAGGCATGGTAGTTCAGAGTCTCCTGAAAGTCGTTTTTCGCAGGAATTCTCCCATATGATTAACTTGGACTCGAGTTTGCTTATGAGCCCCAATCAATCTAGAAGATGGCGTCGAGCAAACTCTGATTCTGAAAATGATGGTTTAGATAGTATTGACTCTATGTTTCGAGAGAGTGGATCAAATTTCAGTTTTGGGTCTTACAGACATTCTTATGGTGATATTGATTTTGTTTCGTACGGTACTTATGGGGGTGACTCGGACGCTTCCATGGATGGACGAAGTTCCTTGGACACAGATGTTTTCGTTTTTCCTGGTGATGGATTCAATGATACCGATATAGACATTGATCCTATGCGTGCTGGTGTCGGCGATTGGGACTCGAGTGATGAGGAGGAGGATCTTGGTGAATTAACAGAAGCTGACACAGAGCAAGATGCACTCGAAGTAGAATCTTCTCAAGCTAGGCCTCCACTTCAAGATTTTCAAGTTTCTAGTTCTTCTGGGAGAGTTAACTCTGGTAATTGGAATGAACAACTTTCTTCACCAGAATACGAACTCGGGCGAATCAGGAGGAATGGGCGATATTATTCGAACATGAACTATTCACAGTCGGAATTACTATCTTTCGTTGAGAATTTTGGTGAATATCTTGACCAACAAGGCTTTGAACAACTTCTTGAGCAAATTGCCATGACGACCAGCTCAAGACGCGGAGCACCTCCTGCAGCTGTCTCTTCAGTGAAAAATCTCCCACTTTTGGTGATTTCTAAAGAACATTTGAAACATGACAATATATCCTGTGCAATTTGTAAGGATTTCTTACTCGTGGGCGTCGAAGTGAATCAACTACCTTGCCTTCATCTCTACCACCCTTCCTGCATCATGCCGTGGTTGCGTACACGGAATTCGTGCCCCCTTTGTCGATATGAACTTCCAACCGACGATCGAGATTACGAAGAGGGAAAGCAAAGTAGCATCATTACAGCAGAGGTCCAAGGAGTTGGAGCAAATCTAGAGTTTAGAACCAGCCAAGGAGAGGAGCTGAACAACATAGACTCTGCAGTTAATAACAGTAACAATTCAGGCAGACACGACGGTATAAGAAGTTGGGTGTTCGTGGCTGCTCCGATCGTTGGTCTTGCAGGTATAGCCCTGATGTTATGGTTTGGTAGCCCTCGGTGTGATCAAAGAAGAGAACAGAAGCAGGAGATGGTGGTGAATTGTCTAGCAATAGCGTATGCGTTCGAGTATCCTTGGAGCGGCGAACGATGGTGGTTTTCTTCAGCATTTAGTCTATTGTTTTCGGCCAATATCCGATGGAGAAGGAATCATCGATTACATAATTGGAATTTGGCGTTCTCCTTTGGGTGGAAAATCCGAAAAGCTCAATTTTTTTGTCAAGTTATTTCTTGTAGTCAAGTAGTCTAG

Protein sequence

MLEMDIDLQRILEEFLQSTAHVMDNNELVDEHGLNQSPTCACCERFLVLDNDVSGEPEAIGICGDCKFLLLEDMEFREQDSYNRAMPRGRRTRHGSSESPESRFSQEFSHMINLDSSLLMSPNQSRRWRRANSDSENDGLDSIDSMFRESGSNFSFGSYRHSYGDIDFVSYGTYGGDSDASMDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGVGDWDSSDEEEDLGELTEADTEQDALEVESSQARPPLQDFQVSSSSGRVNSGNWNEQLSSPEYELGRIRRNGRYYSNMNYSQSELLSFVENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAAVSSVKNLPLLVISKEHLKHDNISCAICKDFLLVGVEVNQLPCLHLYHPSCIMPWLRTRNSCPLCRYELPTDDRDYEEGKQSSIITAEVQGVGANLEFRTSQGEELNNIDSAVNNSNNSGRHDGIRSWVFVAAPIVGLAGIALMLWFGSPRCDQRREQKQEMVVNCLAIAYAFEYPWSGERWWFSSAFSLLFSANIRWRRNHRLHNWNLAFSFGWKIRKAQFFCQVISCSQVV
Homology
BLAST of Csor.00g127540 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 6.3e-28
Identity = 55/122 (45.08%), Postives = 79/122 (64.75%), Query Frame = 0

Query: 304 NFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAAVSSVKNLPLLVISKEHLKHDNISCAICK 363
           NFG+Y    G EQL++Q+A    +R G PPA+ S++  LP + ++K+ LK +   CA+C 
Sbjct: 161 NFGDYFFGPGLEQLIQQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCM 220

Query: 364 DFLLVGVEVNQLPCLHLYHPSCIMPWLRTRNSCPLCRYELPTDDRDYEEGKQSSIITAEV 423
           D    G +V Q+PC H++H  C++PWL   NSCP+CR+ELPTDD DYE   Q S  + + 
Sbjct: 221 DEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDPDYENRSQGSQGSGDG 280

Query: 424 QG 426
           QG
Sbjct: 281 QG 282

BLAST of Csor.00g127540 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 4.1e-27
Identity = 53/108 (49.07%), Postives = 72/108 (66.67%), Query Frame = 0

Query: 304 NFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAAVSSVKNLPLLVISKEHLKHDNISCAICK 363
           N G+Y    G EQL++Q+A    +R G PPA+ S+++ LPL+ I+K +L  +   CA+C 
Sbjct: 169 NIGDYFIGPGLEQLIQQLAENDPNRYGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCM 228

Query: 364 DFLLVGVEVNQLPCLHLYHPSCIMPWLRTRNSCPLCRYELPTDDRDYE 412
           D    G E  Q+PC HLYH  C++PWL   NSCP+CR+ELPTDD DYE
Sbjct: 229 DDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPTDDPDYE 276

BLAST of Csor.00g127540 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 4.0e-22
Identity = 53/134 (39.55%), Postives = 74/134 (55.22%), Query Frame = 0

Query: 304 NFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAAVSSVKNLPLLVISKEHLKHDNISCAICK 363
           N G+Y    G E+L EQ++  T +RRG PPA  S++  LP + I++ HL+  + +C +CK
Sbjct: 136 NTGDYFFGPGLEELFEQLSAGT-TRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCK 195

Query: 364 DFLLVGVEVNQLPCLHLYHPSCIMPWLRTRNSCPLCRYELP------------TDDRDYE 423
           D   +G E  Q+PC H+YH  CI+PWL   NSCP+CR ELP            T  R+Y 
Sbjct: 196 DEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGPSSSQNRTTPTRNYR 255

Query: 424 EGKQSSIITAEVQG 426
               SS   +   G
Sbjct: 256 SSSSSSSSNSRENG 268

BLAST of Csor.00g127540 vs. ExPASy Swiss-Prot
Match: Q9SPL2 (E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 2.0e-21
Identity = 83/239 (34.73%), Postives = 115/239 (48.12%), Query Frame = 0

Query: 187 SLDTDVFVFPGDGFNDTDIDIDPMRAGVGDWDSSDEEEDLGELTEADTEQDALEVESSQA 246
           S + D F    +  N+ D D D      GD +  DEEE+L    E D E D       + 
Sbjct: 101 SYEDDFFRLELNSRNEIDDDEDEDEDD-GDEEEEDEEENLTVNDEEDEEDDL----RRRN 160

Query: 247 RPPLQDFQVSSSSGRVNSGNWNEQLSSPEYELGRIRRNGRYYSNMNYSQSELLSFVENFG 306
           R PL   Q  S +GR    +W E L   E      R     Y+              N  
Sbjct: 161 RFPLTTTQ--SRTGRNRILDWAEILMGIEDNSIEFRMESDRYAG-------------NPA 220

Query: 307 EYLDQ-QGFEQLLEQIAMTT----SSRRGAPPAAVSSVKNLPLLVISKEHLKHDNISCAI 366
           +Y+D   G+E LL+ +A         RRGAPPAA S+++ L    +S    +   + CA+
Sbjct: 221 DYIDDAAGYEALLQNLAEGDGGGGGGRRGAPPAAKSAIEALETFEVSSSEGEMVMV-CAV 280

Query: 367 CKDFLLVGVEVNQLPCLHLYHPSCIMPWLRTRNSCPLCRYELPTDDRDYEEGKQSSIIT 421
           CKD +++G    +LPC H YH  CI+PWL TRNSCP+CR++L TDD +YEE ++    T
Sbjct: 281 CKDGMVMGETGKKLPCGHCYHGDCIVPWLGTRNSCPVCRFQLETDDAEYEEERKKRTST 318

BLAST of Csor.00g127540 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 3.4e-21
Identity = 51/114 (44.74%), Postives = 69/114 (60.53%), Query Frame = 0

Query: 304 NFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAAVSSVKNLPLLVISKEHLKHDNISCAICK 363
           N G+Y    G E+L+EQ++  T   RG PPA  SS+  LP + I+++HLK  +  C +CK
Sbjct: 132 NAGDYFFGPGLEELIEQLSSGT-HHRGPPPAPKSSIDALPTIKITQKHLKSSDSHCPVCK 191

Query: 364 DFLLVGVEVNQLPCLHLYHPSCIMPWLRTRNSCPLCRYELPTDDRDYEEGKQSS 418
           D   +  E  Q+PC H+YH  CI+PWL   NSCP+CR ELP+  R      QSS
Sbjct: 192 DEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPS--RGSSSSTQSS 242

BLAST of Csor.00g127540 vs. NCBI nr
Match: KAG6595533.1 (E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1146 bits (2965), Expect = 0.0
Identity = 567/567 (100.00%), Postives = 567/567 (100.00%), Query Frame = 0

Query: 1   MLEMDIDLQRILEEFLQSTAHVMDNNELVDEHGLNQSPTCACCERFLVLDNDVSGEPEAI 60
           MLEMDIDLQRILEEFLQSTAHVMDNNELVDEHGLNQSPTCACCERFLVLDNDVSGEPEAI
Sbjct: 1   MLEMDIDLQRILEEFLQSTAHVMDNNELVDEHGLNQSPTCACCERFLVLDNDVSGEPEAI 60

Query: 61  GICGDCKFLLLEDMEFREQDSYNRAMPRGRRTRHGSSESPESRFSQEFSHMINLDSSLLM 120
           GICGDCKFLLLEDMEFREQDSYNRAMPRGRRTRHGSSESPESRFSQEFSHMINLDSSLLM
Sbjct: 61  GICGDCKFLLLEDMEFREQDSYNRAMPRGRRTRHGSSESPESRFSQEFSHMINLDSSLLM 120

Query: 121 SPNQSRRWRRANSDSENDGLDSIDSMFRESGSNFSFGSYRHSYGDIDFVSYGTYGGDSDA 180
           SPNQSRRWRRANSDSENDGLDSIDSMFRESGSNFSFGSYRHSYGDIDFVSYGTYGGDSDA
Sbjct: 121 SPNQSRRWRRANSDSENDGLDSIDSMFRESGSNFSFGSYRHSYGDIDFVSYGTYGGDSDA 180

Query: 181 SMDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGVGDWDSSDEEEDLGELTEADTEQDALE 240
           SMDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGVGDWDSSDEEEDLGELTEADTEQDALE
Sbjct: 181 SMDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGVGDWDSSDEEEDLGELTEADTEQDALE 240

Query: 241 VESSQARPPLQDFQVSSSSGRVNSGNWNEQLSSPEYELGRIRRNGRYYSNMNYSQSELLS 300
           VESSQARPPLQDFQVSSSSGRVNSGNWNEQLSSPEYELGRIRRNGRYYSNMNYSQSELLS
Sbjct: 241 VESSQARPPLQDFQVSSSSGRVNSGNWNEQLSSPEYELGRIRRNGRYYSNMNYSQSELLS 300

Query: 301 FVENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAAVSSVKNLPLLVISKEHLKHDNISCA 360
           FVENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAAVSSVKNLPLLVISKEHLKHDNISCA
Sbjct: 301 FVENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAAVSSVKNLPLLVISKEHLKHDNISCA 360

Query: 361 ICKDFLLVGVEVNQLPCLHLYHPSCIMPWLRTRNSCPLCRYELPTDDRDYEEGKQSSIIT 420
           ICKDFLLVGVEVNQLPCLHLYHPSCIMPWLRTRNSCPLCRYELPTDDRDYEEGKQSSIIT
Sbjct: 361 ICKDFLLVGVEVNQLPCLHLYHPSCIMPWLRTRNSCPLCRYELPTDDRDYEEGKQSSIIT 420

Query: 421 AEVQGVGANLEFRTSQGEELNNIDSAVNNSNNSGRHDGIRSWVFVAAPIVGLAGIALMLW 480
           AEVQGVGANLEFRTSQGEELNNIDSAVNNSNNSGRHDGIRSWVFVAAPIVGLAGIALMLW
Sbjct: 421 AEVQGVGANLEFRTSQGEELNNIDSAVNNSNNSGRHDGIRSWVFVAAPIVGLAGIALMLW 480

Query: 481 FGSPRCDQRREQKQEMVVNCLAIAYAFEYPWSGERWWFSSAFSLLFSANIRWRRNHRLHN 540
           FGSPRCDQRREQKQEMVVNCLAIAYAFEYPWSGERWWFSSAFSLLFSANIRWRRNHRLHN
Sbjct: 481 FGSPRCDQRREQKQEMVVNCLAIAYAFEYPWSGERWWFSSAFSLLFSANIRWRRNHRLHN 540

Query: 541 WNLAFSFGWKIRKAQFFCQVISCSQVV 567
           WNLAFSFGWKIRKAQFFCQVISCSQVV
Sbjct: 541 WNLAFSFGWKIRKAQFFCQVISCSQVV 567

BLAST of Csor.00g127540 vs. NCBI nr
Match: KAG7027514.1 (E3 ubiquitin-protein ligase RING1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 961 bits (2484), Expect = 0.0
Identity = 485/515 (94.17%), Postives = 489/515 (94.95%), Query Frame = 0

Query: 1   MLEMDIDLQRILEEFLQSTAHVMDNNELVDEHGLNQSPTCACCERFLVLDNDVSGEPEAI 60
           MLEMDIDLQRILEEFLQSTAHVMDNNELVDEHGLNQSPTCACCERFLVLDNDVSGEPEAI
Sbjct: 6   MLEMDIDLQRILEEFLQSTAHVMDNNELVDEHGLNQSPTCACCERFLVLDNDVSGEPEAI 65

Query: 61  GICGDCKFLLLEDMEFREQDSYNRAMPRGRRTRHGSSESPESRFSQEFSHMINL------ 120
           GICGDCKFLLLEDMEFREQDSYNRA+PRGRRTRHGSSESPESRFSQEFSHMINL      
Sbjct: 66  GICGDCKFLLLEDMEFREQDSYNRALPRGRRTRHGSSESPESRFSQEFSHMINLVRQSQS 125

Query: 121 --------------------DSSLLMSPNQSRRWRRANSDSENDGLDSIDSMFRESGSNF 180
                               DSSLLMSPNQSRRWRRANSDSENDGLDSIDSMFRESGSNF
Sbjct: 126 SISSYGDRYADGSNATYATQDSSLLMSPNQSRRWRRANSDSENDGLDSIDSMFRESGSNF 185

Query: 181 SFGSYRHSYGDIDFVSYGTYGGDSDASMDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGV 240
           SFGSYRHSYGDIDFVSYGTYGGDSDASMDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGV
Sbjct: 186 SFGSYRHSYGDIDFVSYGTYGGDSDASMDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGV 245

Query: 241 GDWDSSDEEEDLGELTEADTEQDALEVESSQARPPLQDFQVSSSSGRVNSGNWNEQLSSP 300
           GDWDSSDEEEDLGELTEADTEQDALEVESSQ+RPPLQDFQVSSSSGRVNSGNWNEQLSSP
Sbjct: 246 GDWDSSDEEEDLGELTEADTEQDALEVESSQSRPPLQDFQVSSSSGRVNSGNWNEQLSSP 305

Query: 301 EYELGRIRRNGRYYSNMNYSQSELLSFVENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPA 360
           EYELGRIRRNGR+YSNMNYSQSELLSFVENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPA
Sbjct: 306 EYELGRIRRNGRFYSNMNYSQSELLSFVENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPA 365

Query: 361 AVSSVKNLPLLVISKEHLKHDNISCAICKDFLLVGVEVNQLPCLHLYHPSCIMPWLRTRN 420
           AVSSVKNLPLLVISKEHLKHDNISCAICKDFLLVGVEVNQLPCLHLYHPSCIMPWLRTRN
Sbjct: 366 AVSSVKNLPLLVISKEHLKHDNISCAICKDFLLVGVEVNQLPCLHLYHPSCIMPWLRTRN 425

Query: 421 SCPLCRYELPTDDRDYEEGKQSSIITAEVQGVGANLEFRTSQGEELNNIDSAVNNSNNSG 480
           SCPLCRYELPTDDRDYEEGK+SSIITAEVQGVGANLEFRTSQGEELNNIDSAVNNSNNSG
Sbjct: 426 SCPLCRYELPTDDRDYEEGKRSSIITAEVQGVGANLEFRTSQGEELNNIDSAVNNSNNSG 485

Query: 481 RHDGIRSWVFVAAPIVGLAGIALMLWFGSPRCDQR 489
           RHDGIRSWVFVAAPIVGLAGIALMLWFGSPRCDQR
Sbjct: 486 RHDGIRSWVFVAAPIVGLAGIALMLWFGSPRCDQR 520

BLAST of Csor.00g127540 vs. NCBI nr
Match: XP_022924891.1 (uncharacterized protein LOC111432303 isoform X4 [Cucurbita moschata])

HSP 1 Score: 955 bits (2469), Expect = 0.0
Identity = 477/488 (97.75%), Postives = 481/488 (98.57%), Query Frame = 0

Query: 2   LEMDIDLQRILEEFLQSTAHVMDNNELVDEHGLNQSPTCACCERFLVLDNDVSGEPEAIG 61
           + MDIDLQRILEEFLQSTAHVMDNNELVDEHGLNQSPTCACCERFLVLDNDVSGEPEAIG
Sbjct: 8   MRMDIDLQRILEEFLQSTAHVMDNNELVDEHGLNQSPTCACCERFLVLDNDVSGEPEAIG 67

Query: 62  ICGDCKFLLLEDMEFREQDSYNRAMPRGRRTRHGSSESPESRFSQEFSHMINLDSSLLMS 121
           ICGDCKFLLLEDMEF EQDSYNRAMPRGRRTRHGSSESPESRFSQEFSHMINLDSSLLMS
Sbjct: 68  ICGDCKFLLLEDMEFPEQDSYNRAMPRGRRTRHGSSESPESRFSQEFSHMINLDSSLLMS 127

Query: 122 PNQSRRWRRANSDSENDGLDSIDSMFRESGSNFSFGSYRHSYGDIDFVSYGTYGGDSDAS 181
           PNQSRRWRRANSDSENDGLDSIDSMFRESGSNFSFGSYRHS G IDFVSYGTYGGDSDAS
Sbjct: 128 PNQSRRWRRANSDSENDGLDSIDSMFRESGSNFSFGSYRHSSGGIDFVSYGTYGGDSDAS 187

Query: 182 MDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGVGDWDSSDEEEDLGELTEADTEQDALEV 241
           MDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGVGDWDSSDEEEDLGELTEADTEQDALEV
Sbjct: 188 MDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGVGDWDSSDEEEDLGELTEADTEQDALEV 247

Query: 242 ESSQARPPLQDFQVSSSSGRVNSGNWNEQLSSPEYELGRIRRNGRYYSNMNYSQSELLSF 301
           ESSQARPPLQDFQVSS+SGRVNSGNWNEQLSSPEYEL RIRRNGR+YSNMNYSQSELLSF
Sbjct: 248 ESSQARPPLQDFQVSSASGRVNSGNWNEQLSSPEYELERIRRNGRFYSNMNYSQSELLSF 307

Query: 302 VENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAAVSSVKNLPLLVISKEHLKHDNISCAI 361
           VENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAAVSSVKNLPLLVISKEHLKHDNISCAI
Sbjct: 308 VENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAAVSSVKNLPLLVISKEHLKHDNISCAI 367

Query: 362 CKDFLLVGVEVNQLPCLHLYHPSCIMPWLRTRNSCPLCRYELPTDDRDYEEGKQSSIITA 421
           CKDFLLVGV VNQLPCLHLYHPSCIMPWLRTRNSCPLCRYELPTDDRDYEEGK+SSI TA
Sbjct: 368 CKDFLLVGVAVNQLPCLHLYHPSCIMPWLRTRNSCPLCRYELPTDDRDYEEGKRSSINTA 427

Query: 422 EVQGVGANLEFRTSQGEELNNIDSAVNNSNNSGRHDGIRSWVFVAAPIVGLAGIALMLWF 481
           EVQGVGANLEFRTSQGEELNNIDSAVNNSNNSGRHDGIRSWVFVAAPIVGLAGIALMLWF
Sbjct: 428 EVQGVGANLEFRTSQGEELNNIDSAVNNSNNSGRHDGIRSWVFVAAPIVGLAGIALMLWF 487

Query: 482 GSPRCDQR 489
           GSPRCDQR
Sbjct: 488 GSPRCDQR 495

BLAST of Csor.00g127540 vs. NCBI nr
Match: XP_022924889.1 (E3 ubiquitin-protein ligase Praja-2-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 945 bits (2443), Expect = 0.0
Identity = 480/515 (93.20%), Postives = 483/515 (93.79%), Query Frame = 0

Query: 1   MLEMDIDLQRILEEFLQSTAHVMDNNELVDEHGLNQSPTCACCERFLVLDNDVSGEPEAI 60
           MLEMDIDLQRILEEFLQSTAHVMDNNELVDEHGLNQSPTCACCERFLVLDNDVSGEPEAI
Sbjct: 6   MLEMDIDLQRILEEFLQSTAHVMDNNELVDEHGLNQSPTCACCERFLVLDNDVSGEPEAI 65

Query: 61  GICGDCKFLLLEDMEFREQDSYNRAMPRGRRTRHGSSESPESRFSQEFSHMINL------ 120
           GICGDCKFLLLEDMEF EQDSYNRAMPRGRRTRHGSSESPESRFSQEFSHMINL      
Sbjct: 66  GICGDCKFLLLEDMEFPEQDSYNRAMPRGRRTRHGSSESPESRFSQEFSHMINLVRQSQS 125

Query: 121 --------------------DSSLLMSPNQSRRWRRANSDSENDGLDSIDSMFRESGSNF 180
                               DSSLLMSPNQSRRWRRANSDSENDGLDSIDSMFRESGSNF
Sbjct: 126 SISSYGDRYADGSNATYAMQDSSLLMSPNQSRRWRRANSDSENDGLDSIDSMFRESGSNF 185

Query: 181 SFGSYRHSYGDIDFVSYGTYGGDSDASMDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGV 240
           SFGSYRHS G IDFVSYGTYGGDSDASMDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGV
Sbjct: 186 SFGSYRHSSGGIDFVSYGTYGGDSDASMDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGV 245

Query: 241 GDWDSSDEEEDLGELTEADTEQDALEVESSQARPPLQDFQVSSSSGRVNSGNWNEQLSSP 300
           GDWDSSDEEEDLGELTEADTEQDALEVESSQARPPLQDFQVSS+SGRVNSGNWNEQLSSP
Sbjct: 246 GDWDSSDEEEDLGELTEADTEQDALEVESSQARPPLQDFQVSSASGRVNSGNWNEQLSSP 305

Query: 301 EYELGRIRRNGRYYSNMNYSQSELLSFVENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPA 360
           EYEL RIRRNGR+YSNMNYSQSELLSFVENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPA
Sbjct: 306 EYELERIRRNGRFYSNMNYSQSELLSFVENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPA 365

Query: 361 AVSSVKNLPLLVISKEHLKHDNISCAICKDFLLVGVEVNQLPCLHLYHPSCIMPWLRTRN 420
           AVSSVKNLPLLVISKEHLKHDNISCAICKDFLLVGV VNQLPCLHLYHPSCIMPWLRTRN
Sbjct: 366 AVSSVKNLPLLVISKEHLKHDNISCAICKDFLLVGVAVNQLPCLHLYHPSCIMPWLRTRN 425

Query: 421 SCPLCRYELPTDDRDYEEGKQSSIITAEVQGVGANLEFRTSQGEELNNIDSAVNNSNNSG 480
           SCPLCRYELPTDDRDYEEGK+SSI TAEVQGVGANLEFRTSQGEELNNIDSAVNNSNNSG
Sbjct: 426 SCPLCRYELPTDDRDYEEGKRSSINTAEVQGVGANLEFRTSQGEELNNIDSAVNNSNNSG 485

Query: 481 RHDGIRSWVFVAAPIVGLAGIALMLWFGSPRCDQR 489
           RHDGIRSWVFVAAPIVGLAGIALMLWFGSPRCDQR
Sbjct: 486 RHDGIRSWVFVAAPIVGLAGIALMLWFGSPRCDQR 520

BLAST of Csor.00g127540 vs. NCBI nr
Match: XP_022924884.1 (E3 ubiquitin-protein ligase Praja-2-like isoform X1 [Cucurbita moschata] >XP_022924886.1 E3 ubiquitin-protein ligase Praja-2-like isoform X1 [Cucurbita moschata] >XP_022924887.1 E3 ubiquitin-protein ligase Praja-2-like isoform X1 [Cucurbita moschata] >XP_022924888.1 E3 ubiquitin-protein ligase Praja-2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 941 bits (2432), Expect = 0.0
Identity = 477/514 (92.80%), Postives = 481/514 (93.58%), Query Frame = 0

Query: 2   LEMDIDLQRILEEFLQSTAHVMDNNELVDEHGLNQSPTCACCERFLVLDNDVSGEPEAIG 61
           + MDIDLQRILEEFLQSTAHVMDNNELVDEHGLNQSPTCACCERFLVLDNDVSGEPEAIG
Sbjct: 8   MRMDIDLQRILEEFLQSTAHVMDNNELVDEHGLNQSPTCACCERFLVLDNDVSGEPEAIG 67

Query: 62  ICGDCKFLLLEDMEFREQDSYNRAMPRGRRTRHGSSESPESRFSQEFSHMINL------- 121
           ICGDCKFLLLEDMEF EQDSYNRAMPRGRRTRHGSSESPESRFSQEFSHMINL       
Sbjct: 68  ICGDCKFLLLEDMEFPEQDSYNRAMPRGRRTRHGSSESPESRFSQEFSHMINLVRQSQSS 127

Query: 122 -------------------DSSLLMSPNQSRRWRRANSDSENDGLDSIDSMFRESGSNFS 181
                              DSSLLMSPNQSRRWRRANSDSENDGLDSIDSMFRESGSNFS
Sbjct: 128 ISSYGDRYADGSNATYAMQDSSLLMSPNQSRRWRRANSDSENDGLDSIDSMFRESGSNFS 187

Query: 182 FGSYRHSYGDIDFVSYGTYGGDSDASMDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGVG 241
           FGSYRHS G IDFVSYGTYGGDSDASMDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGVG
Sbjct: 188 FGSYRHSSGGIDFVSYGTYGGDSDASMDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGVG 247

Query: 242 DWDSSDEEEDLGELTEADTEQDALEVESSQARPPLQDFQVSSSSGRVNSGNWNEQLSSPE 301
           DWDSSDEEEDLGELTEADTEQDALEVESSQARPPLQDFQVSS+SGRVNSGNWNEQLSSPE
Sbjct: 248 DWDSSDEEEDLGELTEADTEQDALEVESSQARPPLQDFQVSSASGRVNSGNWNEQLSSPE 307

Query: 302 YELGRIRRNGRYYSNMNYSQSELLSFVENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAA 361
           YEL RIRRNGR+YSNMNYSQSELLSFVENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAA
Sbjct: 308 YELERIRRNGRFYSNMNYSQSELLSFVENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAA 367

Query: 362 VSSVKNLPLLVISKEHLKHDNISCAICKDFLLVGVEVNQLPCLHLYHPSCIMPWLRTRNS 421
           VSSVKNLPLLVISKEHLKHDNISCAICKDFLLVGV VNQLPCLHLYHPSCIMPWLRTRNS
Sbjct: 368 VSSVKNLPLLVISKEHLKHDNISCAICKDFLLVGVAVNQLPCLHLYHPSCIMPWLRTRNS 427

Query: 422 CPLCRYELPTDDRDYEEGKQSSIITAEVQGVGANLEFRTSQGEELNNIDSAVNNSNNSGR 481
           CPLCRYELPTDDRDYEEGK+SSI TAEVQGVGANLEFRTSQGEELNNIDSAVNNSNNSGR
Sbjct: 428 CPLCRYELPTDDRDYEEGKRSSINTAEVQGVGANLEFRTSQGEELNNIDSAVNNSNNSGR 487

Query: 482 HDGIRSWVFVAAPIVGLAGIALMLWFGSPRCDQR 489
           HDGIRSWVFVAAPIVGLAGIALMLWFGSPRCDQR
Sbjct: 488 HDGIRSWVFVAAPIVGLAGIALMLWFGSPRCDQR 521

BLAST of Csor.00g127540 vs. ExPASy TrEMBL
Match: A0A6J1EAQ0 (uncharacterized protein LOC111432303 isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111432303 PE=4 SV=1)

HSP 1 Score: 955 bits (2469), Expect = 0.0
Identity = 477/488 (97.75%), Postives = 481/488 (98.57%), Query Frame = 0

Query: 2   LEMDIDLQRILEEFLQSTAHVMDNNELVDEHGLNQSPTCACCERFLVLDNDVSGEPEAIG 61
           + MDIDLQRILEEFLQSTAHVMDNNELVDEHGLNQSPTCACCERFLVLDNDVSGEPEAIG
Sbjct: 8   MRMDIDLQRILEEFLQSTAHVMDNNELVDEHGLNQSPTCACCERFLVLDNDVSGEPEAIG 67

Query: 62  ICGDCKFLLLEDMEFREQDSYNRAMPRGRRTRHGSSESPESRFSQEFSHMINLDSSLLMS 121
           ICGDCKFLLLEDMEF EQDSYNRAMPRGRRTRHGSSESPESRFSQEFSHMINLDSSLLMS
Sbjct: 68  ICGDCKFLLLEDMEFPEQDSYNRAMPRGRRTRHGSSESPESRFSQEFSHMINLDSSLLMS 127

Query: 122 PNQSRRWRRANSDSENDGLDSIDSMFRESGSNFSFGSYRHSYGDIDFVSYGTYGGDSDAS 181
           PNQSRRWRRANSDSENDGLDSIDSMFRESGSNFSFGSYRHS G IDFVSYGTYGGDSDAS
Sbjct: 128 PNQSRRWRRANSDSENDGLDSIDSMFRESGSNFSFGSYRHSSGGIDFVSYGTYGGDSDAS 187

Query: 182 MDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGVGDWDSSDEEEDLGELTEADTEQDALEV 241
           MDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGVGDWDSSDEEEDLGELTEADTEQDALEV
Sbjct: 188 MDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGVGDWDSSDEEEDLGELTEADTEQDALEV 247

Query: 242 ESSQARPPLQDFQVSSSSGRVNSGNWNEQLSSPEYELGRIRRNGRYYSNMNYSQSELLSF 301
           ESSQARPPLQDFQVSS+SGRVNSGNWNEQLSSPEYEL RIRRNGR+YSNMNYSQSELLSF
Sbjct: 248 ESSQARPPLQDFQVSSASGRVNSGNWNEQLSSPEYELERIRRNGRFYSNMNYSQSELLSF 307

Query: 302 VENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAAVSSVKNLPLLVISKEHLKHDNISCAI 361
           VENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAAVSSVKNLPLLVISKEHLKHDNISCAI
Sbjct: 308 VENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAAVSSVKNLPLLVISKEHLKHDNISCAI 367

Query: 362 CKDFLLVGVEVNQLPCLHLYHPSCIMPWLRTRNSCPLCRYELPTDDRDYEEGKQSSIITA 421
           CKDFLLVGV VNQLPCLHLYHPSCIMPWLRTRNSCPLCRYELPTDDRDYEEGK+SSI TA
Sbjct: 368 CKDFLLVGVAVNQLPCLHLYHPSCIMPWLRTRNSCPLCRYELPTDDRDYEEGKRSSINTA 427

Query: 422 EVQGVGANLEFRTSQGEELNNIDSAVNNSNNSGRHDGIRSWVFVAAPIVGLAGIALMLWF 481
           EVQGVGANLEFRTSQGEELNNIDSAVNNSNNSGRHDGIRSWVFVAAPIVGLAGIALMLWF
Sbjct: 428 EVQGVGANLEFRTSQGEELNNIDSAVNNSNNSGRHDGIRSWVFVAAPIVGLAGIALMLWF 487

Query: 482 GSPRCDQR 489
           GSPRCDQR
Sbjct: 488 GSPRCDQR 495

BLAST of Csor.00g127540 vs. ExPASy TrEMBL
Match: A0A6J1EAH4 (E3 ubiquitin-protein ligase Praja-2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111432303 PE=4 SV=1)

HSP 1 Score: 945 bits (2443), Expect = 0.0
Identity = 480/515 (93.20%), Postives = 483/515 (93.79%), Query Frame = 0

Query: 1   MLEMDIDLQRILEEFLQSTAHVMDNNELVDEHGLNQSPTCACCERFLVLDNDVSGEPEAI 60
           MLEMDIDLQRILEEFLQSTAHVMDNNELVDEHGLNQSPTCACCERFLVLDNDVSGEPEAI
Sbjct: 6   MLEMDIDLQRILEEFLQSTAHVMDNNELVDEHGLNQSPTCACCERFLVLDNDVSGEPEAI 65

Query: 61  GICGDCKFLLLEDMEFREQDSYNRAMPRGRRTRHGSSESPESRFSQEFSHMINL------ 120
           GICGDCKFLLLEDMEF EQDSYNRAMPRGRRTRHGSSESPESRFSQEFSHMINL      
Sbjct: 66  GICGDCKFLLLEDMEFPEQDSYNRAMPRGRRTRHGSSESPESRFSQEFSHMINLVRQSQS 125

Query: 121 --------------------DSSLLMSPNQSRRWRRANSDSENDGLDSIDSMFRESGSNF 180
                               DSSLLMSPNQSRRWRRANSDSENDGLDSIDSMFRESGSNF
Sbjct: 126 SISSYGDRYADGSNATYAMQDSSLLMSPNQSRRWRRANSDSENDGLDSIDSMFRESGSNF 185

Query: 181 SFGSYRHSYGDIDFVSYGTYGGDSDASMDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGV 240
           SFGSYRHS G IDFVSYGTYGGDSDASMDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGV
Sbjct: 186 SFGSYRHSSGGIDFVSYGTYGGDSDASMDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGV 245

Query: 241 GDWDSSDEEEDLGELTEADTEQDALEVESSQARPPLQDFQVSSSSGRVNSGNWNEQLSSP 300
           GDWDSSDEEEDLGELTEADTEQDALEVESSQARPPLQDFQVSS+SGRVNSGNWNEQLSSP
Sbjct: 246 GDWDSSDEEEDLGELTEADTEQDALEVESSQARPPLQDFQVSSASGRVNSGNWNEQLSSP 305

Query: 301 EYELGRIRRNGRYYSNMNYSQSELLSFVENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPA 360
           EYEL RIRRNGR+YSNMNYSQSELLSFVENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPA
Sbjct: 306 EYELERIRRNGRFYSNMNYSQSELLSFVENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPA 365

Query: 361 AVSSVKNLPLLVISKEHLKHDNISCAICKDFLLVGVEVNQLPCLHLYHPSCIMPWLRTRN 420
           AVSSVKNLPLLVISKEHLKHDNISCAICKDFLLVGV VNQLPCLHLYHPSCIMPWLRTRN
Sbjct: 366 AVSSVKNLPLLVISKEHLKHDNISCAICKDFLLVGVAVNQLPCLHLYHPSCIMPWLRTRN 425

Query: 421 SCPLCRYELPTDDRDYEEGKQSSIITAEVQGVGANLEFRTSQGEELNNIDSAVNNSNNSG 480
           SCPLCRYELPTDDRDYEEGK+SSI TAEVQGVGANLEFRTSQGEELNNIDSAVNNSNNSG
Sbjct: 426 SCPLCRYELPTDDRDYEEGKRSSINTAEVQGVGANLEFRTSQGEELNNIDSAVNNSNNSG 485

Query: 481 RHDGIRSWVFVAAPIVGLAGIALMLWFGSPRCDQR 489
           RHDGIRSWVFVAAPIVGLAGIALMLWFGSPRCDQR
Sbjct: 486 RHDGIRSWVFVAAPIVGLAGIALMLWFGSPRCDQR 520

BLAST of Csor.00g127540 vs. ExPASy TrEMBL
Match: A0A6J1EAH0 (E3 ubiquitin-protein ligase Praja-2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111432303 PE=4 SV=1)

HSP 1 Score: 941 bits (2432), Expect = 0.0
Identity = 477/514 (92.80%), Postives = 481/514 (93.58%), Query Frame = 0

Query: 2   LEMDIDLQRILEEFLQSTAHVMDNNELVDEHGLNQSPTCACCERFLVLDNDVSGEPEAIG 61
           + MDIDLQRILEEFLQSTAHVMDNNELVDEHGLNQSPTCACCERFLVLDNDVSGEPEAIG
Sbjct: 8   MRMDIDLQRILEEFLQSTAHVMDNNELVDEHGLNQSPTCACCERFLVLDNDVSGEPEAIG 67

Query: 62  ICGDCKFLLLEDMEFREQDSYNRAMPRGRRTRHGSSESPESRFSQEFSHMINL------- 121
           ICGDCKFLLLEDMEF EQDSYNRAMPRGRRTRHGSSESPESRFSQEFSHMINL       
Sbjct: 68  ICGDCKFLLLEDMEFPEQDSYNRAMPRGRRTRHGSSESPESRFSQEFSHMINLVRQSQSS 127

Query: 122 -------------------DSSLLMSPNQSRRWRRANSDSENDGLDSIDSMFRESGSNFS 181
                              DSSLLMSPNQSRRWRRANSDSENDGLDSIDSMFRESGSNFS
Sbjct: 128 ISSYGDRYADGSNATYAMQDSSLLMSPNQSRRWRRANSDSENDGLDSIDSMFRESGSNFS 187

Query: 182 FGSYRHSYGDIDFVSYGTYGGDSDASMDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGVG 241
           FGSYRHS G IDFVSYGTYGGDSDASMDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGVG
Sbjct: 188 FGSYRHSSGGIDFVSYGTYGGDSDASMDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGVG 247

Query: 242 DWDSSDEEEDLGELTEADTEQDALEVESSQARPPLQDFQVSSSSGRVNSGNWNEQLSSPE 301
           DWDSSDEEEDLGELTEADTEQDALEVESSQARPPLQDFQVSS+SGRVNSGNWNEQLSSPE
Sbjct: 248 DWDSSDEEEDLGELTEADTEQDALEVESSQARPPLQDFQVSSASGRVNSGNWNEQLSSPE 307

Query: 302 YELGRIRRNGRYYSNMNYSQSELLSFVENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAA 361
           YEL RIRRNGR+YSNMNYSQSELLSFVENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAA
Sbjct: 308 YELERIRRNGRFYSNMNYSQSELLSFVENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAA 367

Query: 362 VSSVKNLPLLVISKEHLKHDNISCAICKDFLLVGVEVNQLPCLHLYHPSCIMPWLRTRNS 421
           VSSVKNLPLLVISKEHLKHDNISCAICKDFLLVGV VNQLPCLHLYHPSCIMPWLRTRNS
Sbjct: 368 VSSVKNLPLLVISKEHLKHDNISCAICKDFLLVGVAVNQLPCLHLYHPSCIMPWLRTRNS 427

Query: 422 CPLCRYELPTDDRDYEEGKQSSIITAEVQGVGANLEFRTSQGEELNNIDSAVNNSNNSGR 481
           CPLCRYELPTDDRDYEEGK+SSI TAEVQGVGANLEFRTSQGEELNNIDSAVNNSNNSGR
Sbjct: 428 CPLCRYELPTDDRDYEEGKRSSINTAEVQGVGANLEFRTSQGEELNNIDSAVNNSNNSGR 487

Query: 482 HDGIRSWVFVAAPIVGLAGIALMLWFGSPRCDQR 489
           HDGIRSWVFVAAPIVGLAGIALMLWFGSPRCDQR
Sbjct: 488 HDGIRSWVFVAAPIVGLAGIALMLWFGSPRCDQR 521

BLAST of Csor.00g127540 vs. ExPASy TrEMBL
Match: A0A6J1EGC2 (E3 ubiquitin-protein ligase Praja-2-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111432303 PE=4 SV=1)

HSP 1 Score: 941 bits (2431), Expect = 0.0
Identity = 477/512 (93.16%), Postives = 480/512 (93.75%), Query Frame = 0

Query: 4   MDIDLQRILEEFLQSTAHVMDNNELVDEHGLNQSPTCACCERFLVLDNDVSGEPEAIGIC 63
           MDIDLQRILEEFLQSTAHVMDNNELVDEHGLNQSPTCACCERFLVLDNDVSGEPEAIGIC
Sbjct: 1   MDIDLQRILEEFLQSTAHVMDNNELVDEHGLNQSPTCACCERFLVLDNDVSGEPEAIGIC 60

Query: 64  GDCKFLLLEDMEFREQDSYNRAMPRGRRTRHGSSESPESRFSQEFSHMINL--------- 123
           GDCKFLLLEDMEF EQDSYNRAMPRGRRTRHGSSESPESRFSQEFSHMINL         
Sbjct: 61  GDCKFLLLEDMEFPEQDSYNRAMPRGRRTRHGSSESPESRFSQEFSHMINLVRQSQSSIS 120

Query: 124 -----------------DSSLLMSPNQSRRWRRANSDSENDGLDSIDSMFRESGSNFSFG 183
                            DSSLLMSPNQSRRWRRANSDSENDGLDSIDSMFRESGSNFSFG
Sbjct: 121 SYGDRYADGSNATYAMQDSSLLMSPNQSRRWRRANSDSENDGLDSIDSMFRESGSNFSFG 180

Query: 184 SYRHSYGDIDFVSYGTYGGDSDASMDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGVGDW 243
           SYRHS G IDFVSYGTYGGDSDASMDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGVGDW
Sbjct: 181 SYRHSSGGIDFVSYGTYGGDSDASMDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGVGDW 240

Query: 244 DSSDEEEDLGELTEADTEQDALEVESSQARPPLQDFQVSSSSGRVNSGNWNEQLSSPEYE 303
           DSSDEEEDLGELTEADTEQDALEVESSQARPPLQDFQVSS+SGRVNSGNWNEQLSSPEYE
Sbjct: 241 DSSDEEEDLGELTEADTEQDALEVESSQARPPLQDFQVSSASGRVNSGNWNEQLSSPEYE 300

Query: 304 LGRIRRNGRYYSNMNYSQSELLSFVENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAAVS 363
           L RIRRNGR+YSNMNYSQSELLSFVENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAAVS
Sbjct: 301 LERIRRNGRFYSNMNYSQSELLSFVENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAAVS 360

Query: 364 SVKNLPLLVISKEHLKHDNISCAICKDFLLVGVEVNQLPCLHLYHPSCIMPWLRTRNSCP 423
           SVKNLPLLVISKEHLKHDNISCAICKDFLLVGV VNQLPCLHLYHPSCIMPWLRTRNSCP
Sbjct: 361 SVKNLPLLVISKEHLKHDNISCAICKDFLLVGVAVNQLPCLHLYHPSCIMPWLRTRNSCP 420

Query: 424 LCRYELPTDDRDYEEGKQSSIITAEVQGVGANLEFRTSQGEELNNIDSAVNNSNNSGRHD 483
           LCRYELPTDDRDYEEGK+SSI TAEVQGVGANLEFRTSQGEELNNIDSAVNNSNNSGRHD
Sbjct: 421 LCRYELPTDDRDYEEGKRSSINTAEVQGVGANLEFRTSQGEELNNIDSAVNNSNNSGRHD 480

Query: 484 GIRSWVFVAAPIVGLAGIALMLWFGSPRCDQR 489
           GIRSWVFVAAPIVGLAGIALMLWFGSPRCDQR
Sbjct: 481 GIRSWVFVAAPIVGLAGIALMLWFGSPRCDQR 512

BLAST of Csor.00g127540 vs. ExPASy TrEMBL
Match: A0A6J1EAA3 (E3 ubiquitin-protein ligase Praja-2-like isoform X5 OS=Cucurbita moschata OX=3662 GN=LOC111432303 PE=4 SV=1)

HSP 1 Score: 775 bits (2000), Expect = 1.72e-278
Identity = 397/431 (92.11%), Postives = 400/431 (92.81%), Query Frame = 0

Query: 85  AMPRGRRTRHGSSESPESRFSQEFSHMINL--------------------------DSSL 144
           AMPRGRRTRHGSSESPESRFSQEFSHMINL                          DSSL
Sbjct: 2   AMPRGRRTRHGSSESPESRFSQEFSHMINLVRQSQSSISSYGDRYADGSNATYAMQDSSL 61

Query: 145 LMSPNQSRRWRRANSDSENDGLDSIDSMFRESGSNFSFGSYRHSYGDIDFVSYGTYGGDS 204
           LMSPNQSRRWRRANSDSENDGLDSIDSMFRESGSNFSFGSYRHS G IDFVSYGTYGGDS
Sbjct: 62  LMSPNQSRRWRRANSDSENDGLDSIDSMFRESGSNFSFGSYRHSSGGIDFVSYGTYGGDS 121

Query: 205 DASMDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGVGDWDSSDEEEDLGELTEADTEQDA 264
           DASMDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGVGDWDSSDEEEDLGELTEADTEQDA
Sbjct: 122 DASMDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGVGDWDSSDEEEDLGELTEADTEQDA 181

Query: 265 LEVESSQARPPLQDFQVSSSSGRVNSGNWNEQLSSPEYELGRIRRNGRYYSNMNYSQSEL 324
           LEVESSQARPPLQDFQVSS+SGRVNSGNWNEQLSSPEYEL RIRRNGR+YSNMNYSQSEL
Sbjct: 182 LEVESSQARPPLQDFQVSSASGRVNSGNWNEQLSSPEYELERIRRNGRFYSNMNYSQSEL 241

Query: 325 LSFVENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAAVSSVKNLPLLVISKEHLKHDNIS 384
           LSFVENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAAVSSVKNLPLLVISKEHLKHDNIS
Sbjct: 242 LSFVENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAAVSSVKNLPLLVISKEHLKHDNIS 301

Query: 385 CAICKDFLLVGVEVNQLPCLHLYHPSCIMPWLRTRNSCPLCRYELPTDDRDYEEGKQSSI 444
           CAICKDFLLVGV VNQLPCLHLYHPSCIMPWLRTRNSCPLCRYELPTDDRDYEEGK+SSI
Sbjct: 302 CAICKDFLLVGVAVNQLPCLHLYHPSCIMPWLRTRNSCPLCRYELPTDDRDYEEGKRSSI 361

Query: 445 ITAEVQGVGANLEFRTSQGEELNNIDSAVNNSNNSGRHDGIRSWVFVAAPIVGLAGIALM 489
            TAEVQGVGANLEFRTSQGEELNNIDSAVNNSNNSGRHDGIRSWVFVAAPIVGLAGIALM
Sbjct: 362 NTAEVQGVGANLEFRTSQGEELNNIDSAVNNSNNSGRHDGIRSWVFVAAPIVGLAGIALM 421

BLAST of Csor.00g127540 vs. TAIR 10
Match: AT5G01980.1 (RING/U-box superfamily protein )

HSP 1 Score: 257.7 bits (657), Expect = 2.2e-68
Identity = 197/504 (39.09%), Postives = 266/504 (52.78%), Query Frame = 0

Query: 18  STAHVMDNNELVDEHGLNQSPTCAC--CERFLVLDNDVSGEPEAIGICGDCKFLLLEDME 77
           S  H MD +        N  P+ AC  C R +      S + E   +C DCKFLLLED  
Sbjct: 7   SNGHPMDISS-------NSIPSSACNLCGRVV---QSASDDLEIFSLCVDCKFLLLEDFG 66

Query: 78  ------FREQDSYNRAMPRGRR-TRHGSSES--PESRFSQEFSHMI-------------- 137
                  R Q    R M R RR TRH SSES       SQ+F+H+I              
Sbjct: 67  TPAPPLARRQTIRRRRMRRRRRTTRHDSSESVGVGDLSSQQFTHLISIARQSLSTVQASE 126

Query: 138 ----NLDSSLLMSPNQSRRWRRANSDSENDGLDSIDSMFRESGSNFSFGSYRHSYGDIDF 197
               +L +S   +P+ S RW R  S+SE+D  D+    F E+ SN SF  YR  + D D 
Sbjct: 127 DLRDDLRTSSHTTPSGSTRW-RLFSESESDDFDN----FGETESNASFSLYRFPHADNDT 186

Query: 198 VSYGTYGGDSDASMDGRSSLDTDVFVFPGDGFN-DTDIDIDPMRAGVGDWDSSDEEEDLG 257
           +S+  YGG+SDAS D  +    D+FV P D  + D D DIDPMRAG+  W+S +E     
Sbjct: 187 ISFSAYGGESDASTDRHA----DIFVQPDDRSDIDFDTDIDPMRAGLNQWNSDEE----- 246

Query: 258 ELTEADTEQDALEVESSQARPPLQDFQVSSSSGRVNSGNWNEQLSSPEYELG---RIRRN 317
                D E +     S  A    +++  S S     S +   +  SPE E     RI   
Sbjct: 247 -----DREWEEGAGPSGVAGTRYRNYLASPS----ESYSSMTRFDSPELERSFRQRIIER 306

Query: 318 GRYYSNMNYSQSELLSF---VENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAAVSSVKN 377
               S   ++  E L F     N G+YLD++GF++LLEQ+A + +SRRGAPPA+VS V+N
Sbjct: 307 RHSLSRNIFTGLEDLDFSPYAANVGDYLDERGFDELLEQLAESDNSRRGAPPASVSCVRN 366

Query: 378 LPLLVISKEHLKHDNISCAICKDFLLVGVEVNQLPCLHLYHPSCIMPWLRTRNSCPLCRY 437
           LP ++I++EH+    + CAICK+   +  E  QLPCLHLYH  CI+PWL  RNSCPLCRY
Sbjct: 367 LPRVIIAEEHVM-KGLVCAICKELFSLRNETTQLPCLHLYHAHCIVPWLSARNSCPLCRY 426

Query: 438 ELPTDDRDYEEGKQSSIITAEVQGVGANLEFRTSQGEELNNIDSAVNNSNNSGRHDGIRS 485
           ELPTDD+DYEEGK++ +   +V    ++ +  T  GEE   ++   + S  S    G   
Sbjct: 427 ELPTDDKDYEEGKRNVL---DVSEDSSSSDDGTESGEE-EYVERGESESGVSRVSRG--R 470

BLAST of Csor.00g127540 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 127.1 bits (318), Expect = 4.5e-29
Identity = 55/122 (45.08%), Postives = 79/122 (64.75%), Query Frame = 0

Query: 304 NFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAAVSSVKNLPLLVISKEHLKHDNISCAICK 363
           NFG+Y    G EQL++Q+A    +R G PPA+ S++  LP + ++K+ LK +   CA+C 
Sbjct: 161 NFGDYFFGPGLEQLIQQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCM 220

Query: 364 DFLLVGVEVNQLPCLHLYHPSCIMPWLRTRNSCPLCRYELPTDDRDYEEGKQSSIITAEV 423
           D    G +V Q+PC H++H  C++PWL   NSCP+CR+ELPTDD DYE   Q S  + + 
Sbjct: 221 DEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDPDYENRSQGSQGSGDG 280

Query: 424 QG 426
           QG
Sbjct: 281 QG 282

BLAST of Csor.00g127540 vs. TAIR 10
Match: AT3G02340.1 (RING/U-box superfamily protein )

HSP 1 Score: 113.6 bits (283), Expect = 5.1e-25
Identity = 56/121 (46.28%), Postives = 76/121 (62.81%), Query Frame = 0

Query: 296 SELLSFVENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAAVSSVKNLPLLVISKEHLKHD 355
           S L  F EN   + D    + +L Q+    +  RG PPAA S +++LP++ ++ E L   
Sbjct: 275 SGLDEFDENHSGHYDA---DAILGQMFDDETGIRGNPPAAKSVIQDLPVVELAVEELDKG 334

Query: 356 NISCAICKDFLLVGVEVNQLPCLHLYHPSCIMPWLRTRNSCPLCRYELPTDDRDYEEGKQ 415
           N  CA+CKD +LV  +V +LPC H YH  CI+PWL  RN+CP+CRYELPTDD +YE  K 
Sbjct: 335 NNVCAVCKDEMLVEEKVRRLPCSHFYHGECIIPWLGIRNTCPVCRYELPTDDLEYERHKS 392

Query: 416 S 417
           S
Sbjct: 395 S 392

BLAST of Csor.00g127540 vs. TAIR 10
Match: AT5G64920.1 (COP1-interacting protein 8 )

HSP 1 Score: 105.5 bits (262), Expect = 1.4e-22
Identity = 83/239 (34.73%), Postives = 115/239 (48.12%), Query Frame = 0

Query: 187 SLDTDVFVFPGDGFNDTDIDIDPMRAGVGDWDSSDEEEDLGELTEADTEQDALEVESSQA 246
           S + D F    +  N+ D D D      GD +  DEEE+L    E D E D       + 
Sbjct: 101 SYEDDFFRLELNSRNEIDDDEDEDEDD-GDEEEEDEEENLTVNDEEDEEDDL----RRRN 160

Query: 247 RPPLQDFQVSSSSGRVNSGNWNEQLSSPEYELGRIRRNGRYYSNMNYSQSELLSFVENFG 306
           R PL   Q  S +GR    +W E L   E      R     Y+              N  
Sbjct: 161 RFPLTTTQ--SRTGRNRILDWAEILMGIEDNSIEFRMESDRYAG-------------NPA 220

Query: 307 EYLDQ-QGFEQLLEQIAMTT----SSRRGAPPAAVSSVKNLPLLVISKEHLKHDNISCAI 366
           +Y+D   G+E LL+ +A         RRGAPPAA S+++ L    +S    +   + CA+
Sbjct: 221 DYIDDAAGYEALLQNLAEGDGGGGGGRRGAPPAAKSAIEALETFEVSSSEGEMVMV-CAV 280

Query: 367 CKDFLLVGVEVNQLPCLHLYHPSCIMPWLRTRNSCPLCRYELPTDDRDYEEGKQSSIIT 421
           CKD +++G    +LPC H YH  CI+PWL TRNSCP+CR++L TDD +YEE ++    T
Sbjct: 281 CKDGMVMGETGKKLPCGHCYHGDCIVPWLGTRNSCPVCRFQLETDDAEYEEERKKRTST 318

BLAST of Csor.00g127540 vs. TAIR 10
Match: AT5G60820.1 (RING/U-box superfamily protein )

HSP 1 Score: 104.4 bits (259), Expect = 3.1e-22
Identity = 92/303 (30.36%), Postives = 141/303 (46.53%), Query Frame = 0

Query: 114 LDSSLLMSPNQSRRWRRANSDSENDGLDSIDSMFRESGSNFSFGSYRHSYGDIDFVSYGT 173
           +DS L+++ N+     R       D + +++S F     +         +G ID      
Sbjct: 136 VDSDLVVNLNEHVDGMRLLGIESGDVIVTVESCFDSDDEDDQEKEESEIWG-IDLNEEDE 195

Query: 174 YGGDSDASMDGRSSLDTDVFVFPGDGFNDTDIDIDPMRAGVGDWDSSDEEEDLGELTEAD 233
           Y  D D + D        +     D     D+ I+       + DS DE E L  L EAD
Sbjct: 196 YVNDDDDNDDEEEDASVTI-PLCWDSLQLEDLGINNEDCEWEEVDSDDEREVLSVLAEAD 255

Query: 234 TEQDALEVESSQARPPLQDFQVSSSSGRVNSGNWNEQLSSPEYELGRIRRNGRYYSNMNY 293
            + +++ V S  A   L+D  +S   G  N G W   L+S   E            N++ 
Sbjct: 256 -DNNSVSV-SVAATISLEDLAISERRGSSNLG-WEVLLNSRSLEF-----------NLDD 315

Query: 294 SQSELLSFV-------ENFGEYLDQQGFEQLLEQIAMTTSSRRGAPPAAVSSVKNLPLLV 353
           ++S L  ++       E++ +YL    +E L E      SS  G PPA+ S +KNL +  
Sbjct: 316 AESNLELYIGDIDHEEEDYEDYLHTTEYEMLFE---AEISSGIGKPPASKSFIKNLKVSP 375

Query: 354 ISKEHLKH---DNISCAICKDFLLVGVEVNQLPCLHLYHPSCIMPWLRTRNSCPLCRYEL 407
           +S E +     D + CA+CK+ ++VG EV +LPC H YH  CI+PWL  RN+CP+CR+EL
Sbjct: 376 LSNEDVMENDDDAVCCAVCKEEMIVGKEVAELPCRHKYHSECIVPWLGIRNTCPVCRFEL 419

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LPN76.3e-2845.08E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19... [more]
P0CH304.1e-2749.07E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV... [more]
O221974.0e-2239.55Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Q9SPL22.0e-2134.73E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV... [more]
Q94AK43.4e-2144.74E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV... [more]
Match NameE-valueIdentityDescription
KAG6595533.10.0100.00E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7027514.10.094.17E3 ubiquitin-protein ligase RING1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022924891.10.097.75uncharacterized protein LOC111432303 isoform X4 [Cucurbita moschata][more]
XP_022924889.10.093.20E3 ubiquitin-protein ligase Praja-2-like isoform X2 [Cucurbita moschata][more]
XP_022924884.10.092.80E3 ubiquitin-protein ligase Praja-2-like isoform X1 [Cucurbita moschata] >XP_022... [more]
Match NameE-valueIdentityDescription
A0A6J1EAQ00.097.75uncharacterized protein LOC111432303 isoform X4 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1EAH40.093.20E3 ubiquitin-protein ligase Praja-2-like isoform X2 OS=Cucurbita moschata OX=366... [more]
A0A6J1EAH00.092.80E3 ubiquitin-protein ligase Praja-2-like isoform X1 OS=Cucurbita moschata OX=366... [more]
A0A6J1EGC20.093.16E3 ubiquitin-protein ligase Praja-2-like isoform X3 OS=Cucurbita moschata OX=366... [more]
A0A6J1EAA31.72e-27892.11E3 ubiquitin-protein ligase Praja-2-like isoform X5 OS=Cucurbita moschata OX=366... [more]
Match NameE-valueIdentityDescription
AT5G01980.12.2e-6839.09RING/U-box superfamily protein [more]
AT3G19950.14.5e-2945.08RING/U-box superfamily protein [more]
AT3G02340.15.1e-2546.28RING/U-box superfamily protein [more]
AT5G64920.11.4e-2234.73COP1-interacting protein 8 [more]
AT5G60820.13.1e-2230.36RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 359..399
e-value: 1.2E-5
score: 34.7
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 358..400
e-value: 3.9E-10
score: 39.9
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 359..400
score: 11.781134
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 320..406
e-value: 2.6E-17
score: 64.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 204..269
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 81..100
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 81..105
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 247..269
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 218..238
NoneNo IPR availablePANTHERPTHR15710E3 UBIQUITIN-PROTEIN LIGASE PRAJAcoord: 296..431
NoneNo IPR availablePANTHERPTHR15710:SF41OS06G0101300 PROTEINcoord: 296..431
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 347..407

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g127540.m01Csor.00g127540.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0061630 ubiquitin protein ligase activity