Csor.00g125720 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g125720
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionGibberellin 20 oxidase 2
LocationCsor_Chr15: 3147224 .. 3149084 (+)
RNA-Seq ExpressionCsor.00g125720
SyntenyCsor.00g125720
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAATGGATTCAAGCAGCCCATTGTTGGTCCTCTCCTCTCAGTTCATATGGCCTAAAGAGGATTTAGTAGACGCATATGATGAGAAGCTCAGTGAGCCACACGTCGACCTTCACGGCTTCCTTAAGGGCGATCAAAAGGCCACCATCGAAGCTTCCCACCTCGTAAGGACAGCCTGCATGAAACATGGCTTCTTCCAAGTTATCAACCATGGCGTGGATCAAACCCTGTTAGCCACCGCCCTTGACAAAATGGGCCCTCTTTTTAACCTCCCTTTCCACCTTAAAACCACGGCCTCTGCCAAAATGTGGGGCTTTTCCACTGCCCATTCTAATCGCTTTTCCTCAAAGCTGCCTTGGAAGGAAACTTTCTCCTTTGGGTTTGACCATTGCAAAACCTCCAATAATGAAGCCCTTGTGGTGGATTTCTTCTCTTCCATCTTAGGCCACGAATTTCAAGAAATAGGGTAAGATGGATCGATTTTAATAATGGGTTTTCTATAAAAATGTGTGTTGTTGTTGTGCAATCCTAACCGTTGTTTGAATAGGGCTTTATATGAGAAGTATTGCGAGGCCATGAGGGACTTGGGGCTTGCATTAACGGAGCTTTTGGGCATAAGTTTGGGCGTGGAGAGGTCACATTTTCGCAAGTTTTTTGAGGATGGAAGCTCCATAATGCGATTGAATAGCTACCCAATATGCGAACAAGGCAGCGTGGCCCTTGGCACAGGGCCTCATTGTGATCCAACAGGCTTGACCATCCTCCACCAGGACCAGGTCGGTGGCCTTGAAGTCTTCGCCGACAACAAATGGCACACCGTTCCCCCATCTCAAAATGCACTCGTTATTAACGTTGGTGACATCTTCATGGTTAGTTCCCCAAACTAAATACACAATCAAACCCTTTTCCTTCTCTCTCTCCTTTACATCCCTCTATTTGTAGGGACGTTAATGATTAATTATCTAAAACAAATTTATAATTTTTTACTCCTTAAAATCATTATCAATCTCGTTACCCAAACTCTTACTTTATAATCATTTTCTGTAATTACCTACTTGAACCACGTCACTCTTATCTTGATAACCATTAAAATCATTATCAATCTCGTTACCCAAACTCTTACTTTATAATCATTTTCTGTAATTACCTACTCGAACCACGTCACTCTTATCTTGATAACCATTTTCTACCATGACTAATAGGATAACCATTAGTGGTTGGTTACCCGATACACACCGTGTGTAATACCATATAAAAATCAACATCCTTTTTTGTTTCTTAAAATAATTATCAATGACGGGTAACTAACTCTTATCTTGATAACCATTTTTTGCAATCACCCAATATGGTAAACATTAGTGTCGGGTTATACACGCAATATATAACATTTACCACATTTATTGCTTAAAGTGATTATTAATGCTTGATTACTCGAAACACACTTGGGGTAACTCTTATCTTGATAATCATTTTCTACCATGGTACAATCAGCACGTAGAGTGGCTATTAATGACAGGTTACGTGACGGCTCATAACTCATATTCAAATTATCACTTAACCTAATTTAGGGAGAACATTTTTGTGAAGGCACTTTGTAATGGGGAATACAAAAGCTGCGTGCACCGGGCGTTGGTGAACAAACACAAGGAGAGGAGGTCGTTGGCATTTTTCCTTTGTCCGAGGAAAGACAAGGTGGTGAGGCCGCCGGAGACACTTGTTGTCGGCGGTGGGTCGAGAAAGTACCCGGATTTTGCCTGGTCGGAGCTGCTTGAATTCACTCAGAAATATTACAGAGCCGATGCTGTAACTCTTCAGAACTTTACCAAATGGGTCGTGTCGGCGAGGCCATGTGATGTTTAG

mRNA sequence

ATGGCAATGGATTCAAGCAGCCCATTGTTGGTCCTCTCCTCTCAGTTCATATGGCCTAAAGAGGATTTAGTAGACGCATATGATGAGAAGCTCAGTGAGCCACACGTCGACCTTCACGGCTTCCTTAAGGGCGATCAAAAGGCCACCATCGAAGCTTCCCACCTCGTAAGGACAGCCTGCATGAAACATGGCTTCTTCCAAGTTATCAACCATGGCGTGGATCAAACCCTGTTAGCCACCGCCCTTGACAAAATGGGCCCTCTTTTTAACCTCCCTTTCCACCTTAAAACCACGGCCTCTGCCAAAATGTGGGGCTTTTCCACTGCCCATTCTAATCGCTTTTCCTCAAAGCTGCCTTGGAAGGAAACTTTCTCCTTTGGGTTTGACCATTGCAAAACCTCCAATAATGAAGCCCTTGTGGTGGATTTCTTCTCTTCCATCTTAGGCCACGAATTTCAAGAAATAGGGGCTTTATATGAGAAGTATTGCGAGGCCATGAGGGACTTGGGGCTTGCATTAACGGAGCTTTTGGGCATAAGTTTGGGCGTGGAGAGGTCACATTTTCGCAAGTTTTTTGAGGATGGAAGCTCCATAATGCGATTGAATAGCTACCCAATATGCGAACAAGGCAGCGTGGCCCTTGGCACAGGGCCTCATTGTGATCCAACAGGCTTGACCATCCTCCACCAGGACCAGGTCGGTGGCCTTGAAGTCTTCGCCGACAACAAATGGCACACCGTTCCCCCATCTCAAAATGCACTCGTTATTAACGTTGGTGACATCTTCATGGCACTTTGTAATGGGGAATACAAAAGCTGCGTGCACCGGGCGTTGGTGAACAAACACAAGGAGAGGAGGTCGTTGGCATTTTTCCTTTGTCCGAGGAAAGACAAGGTGGTGAGGCCGCCGGAGACACTTGTTGTCGGCGGTGGGTCGAGAAAGTACCCGGATTTTGCCTGGTCGGAGCTGCTTGAATTCACTCAGAAATATTACAGAGCCGATGCTGTAACTCTTCAGAACTTTACCAAATGGGTCGTGTCGGCGAGGCCATGTGATGTTTAG

Coding sequence (CDS)

ATGGCAATGGATTCAAGCAGCCCATTGTTGGTCCTCTCCTCTCAGTTCATATGGCCTAAAGAGGATTTAGTAGACGCATATGATGAGAAGCTCAGTGAGCCACACGTCGACCTTCACGGCTTCCTTAAGGGCGATCAAAAGGCCACCATCGAAGCTTCCCACCTCGTAAGGACAGCCTGCATGAAACATGGCTTCTTCCAAGTTATCAACCATGGCGTGGATCAAACCCTGTTAGCCACCGCCCTTGACAAAATGGGCCCTCTTTTTAACCTCCCTTTCCACCTTAAAACCACGGCCTCTGCCAAAATGTGGGGCTTTTCCACTGCCCATTCTAATCGCTTTTCCTCAAAGCTGCCTTGGAAGGAAACTTTCTCCTTTGGGTTTGACCATTGCAAAACCTCCAATAATGAAGCCCTTGTGGTGGATTTCTTCTCTTCCATCTTAGGCCACGAATTTCAAGAAATAGGGGCTTTATATGAGAAGTATTGCGAGGCCATGAGGGACTTGGGGCTTGCATTAACGGAGCTTTTGGGCATAAGTTTGGGCGTGGAGAGGTCACATTTTCGCAAGTTTTTTGAGGATGGAAGCTCCATAATGCGATTGAATAGCTACCCAATATGCGAACAAGGCAGCGTGGCCCTTGGCACAGGGCCTCATTGTGATCCAACAGGCTTGACCATCCTCCACCAGGACCAGGTCGGTGGCCTTGAAGTCTTCGCCGACAACAAATGGCACACCGTTCCCCCATCTCAAAATGCACTCGTTATTAACGTTGGTGACATCTTCATGGCACTTTGTAATGGGGAATACAAAAGCTGCGTGCACCGGGCGTTGGTGAACAAACACAAGGAGAGGAGGTCGTTGGCATTTTTCCTTTGTCCGAGGAAAGACAAGGTGGTGAGGCCGCCGGAGACACTTGTTGTCGGCGGTGGGTCGAGAAAGTACCCGGATTTTGCCTGGTCGGAGCTGCTTGAATTCACTCAGAAATATTACAGAGCCGATGCTGTAACTCTTCAGAACTTTACCAAATGGGTCGTGTCGGCGAGGCCATGTGATGTTTAG

Protein sequence

MAMDSSSPLLVLSSQFIWPKEDLVDAYDEKLSEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVINHGVDQTLLATALDKMGPLFNLPFHLKTTASAKMWGFSTAHSNRFSSKLPWKETFSFGFDHCKTSNNEALVVDFFSSILGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGVERSHFRKFFEDGSSIMRLNSYPICEQGSVALGTGPHCDPTGLTILHQDQVGGLEVFADNKWHTVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPETLVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARPCDV
Homology
BLAST of Csor.00g125720 vs. ExPASy Swiss-Prot
Match: Q39112 (Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=GA20OX3 PE=2 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 2.2e-103
Identity = 183/344 (53.20%), Postives = 234/344 (68.02%), Query Frame = 0

Query: 12  LSSQFIWPKEDLVDAYDEKLSEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVINH 71
           +  QF+WP  +      + L  P +DL GFL GD     EA+ LV  A  KHGFF + NH
Sbjct: 37  IPQQFVWPDHEKPSTDVQPLQVPLIDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNH 96

Query: 72  GVDQTLLATALDKMGPLFNLPFHLKTTASAKMWGFSTAHSN----RFSSKLPWKETFSFG 131
           GVD++LL+ A   M   F  P   K  A  K WG S+ +++    RFSSKLPWKET SF 
Sbjct: 97  GVDESLLSRAYLHMDSFFKAPACEKQKAQRK-WGESSGYASSFVGRFSSKLPWKETLSFK 156

Query: 132 FDHCKTSNNEALVVDFFSSILGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGVERSH 191
           F   +  +++  V DF S  +G  +++ G +Y++Y EAM  L L + ELLG+SLGVER +
Sbjct: 157 FSPEEKIHSQT-VKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRY 216

Query: 192 FRKFFEDGSSIMRLNSYPICEQGSVALGTGPHCDPTGLTILHQDQVGGLEVFADNKWHTV 251
           F++FFED  SI RLN YP C+Q  +ALGTGPHCDPT LTILHQDQVGGL+VF DNKW ++
Sbjct: 217 FKEFFEDSDSIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSI 276

Query: 252 PPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPETLV 311
           PP+ +A V+N+GD FMAL NG YKSC+HRA+VN  +ER++ AFFLCP+ +KVV+PPE LV
Sbjct: 277 PPNPHAFVVNIGDTFMALTNGRYKSCLHRAVVNSERERKTFAFFLCPKGEKVVKPPEELV 336

Query: 312 VG--GGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSAR 350
            G   G RKYPDF WS  LEFTQK+YRAD  TL  F+ W+ + R
Sbjct: 337 NGVKSGERKYPDFTWSMFLEFTQKHYRADMNTLDEFSIWLKNRR 378

BLAST of Csor.00g125720 vs. ExPASy Swiss-Prot
Match: Q39111 (Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 1.3e-100
Identity = 175/357 (49.02%), Postives = 237/357 (66.39%), Query Frame = 0

Query: 4   DSSSPLLV----------LSSQFIWPKEDLVDAYDEKLSEPHVDLHGFLKGDQKATIEAS 63
           D +SPL+           + +QFIWP E+       +L+ P +DL       Q +T+EA 
Sbjct: 24  DQTSPLIFNPSLLNLQSQIPNQFIWPDEEKPSIDIPELNVPFIDL-----SSQDSTLEAP 83

Query: 64  HLVRTACMKHGFFQVINHGVDQTLLATALDKMGPLFNLPFHLKTTASAK---MWGFSTAH 123
            ++  AC KHGFF V+NHGV ++L+A A   M   F++P   K  A  K     G++++ 
Sbjct: 84  RVIAEACTKHGFFLVVNHGVSESLIADAHRLMESFFDMPLAGKQKAQRKPGESCGYASSF 143

Query: 124 SNRFSSKLPWKETFSFGFDHCKTSNNEALVVDFFSSILGHEFQEIGALYEKYCEAMRDLG 183
           + RFS+KLPWKET SF F +   ++    V D+FS  LG EF++ G +Y+ YCEAM  L 
Sbjct: 144 TGRFSTKLPWKETLSFQFSN--DNSGSRTVQDYFSDTLGQEFEQFGKVYQDYCEAMSSLS 203

Query: 184 LALTELLGISLGVERSHFRKFFEDGSSIMRLNSYPICEQGSVALGTGPHCDPTGLTILHQ 243
           L + ELLG+SLGV R +FR FFE+  SIMRLN YP C+   + LGTGPHCDP+ LTILHQ
Sbjct: 204 LKIMELLGLSLGVNRDYFRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQ 263

Query: 244 DQVGGLEVFADNKWHTVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAF 303
           D V GL+VF DN+W ++ P+  A V+N+GD FMAL NG +KSC+HRA+VN+   R+S+AF
Sbjct: 264 DHVNGLQVFVDNQWQSIRPNPKAFVVNIGDTFMALSNGIFKSCLHRAVVNRESARKSMAF 323

Query: 304 FLCPRKDKVVRPPETLVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVS 348
           FLCP+KDKVV+PP  ++    +RKYPDF WS  LEFTQK+YRAD  TL +F+ WV++
Sbjct: 324 FLCPKKDKVVKPPSDILEKMKTRKYPDFTWSMFLEFTQKHYRADVNTLDSFSNWVIT 373

BLAST of Csor.00g125720 vs. ExPASy Swiss-Prot
Match: Q39110 (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 365.5 bits (937), Expect = 6.6e-100
Identity = 171/337 (50.74%), Postives = 227/337 (67.36%), Query Frame = 0

Query: 12  LSSQFIWPKEDLVDAYDEKLSEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVINH 71
           + +QFIWP ++       +L  P +DL   L  D  +T++AS L+  AC KHGFF V+NH
Sbjct: 40  IPNQFIWPDDEKPSINVLELDVPLIDLQNLL-SDPSSTLDASRLISEACKKHGFFLVVNH 99

Query: 72  GVDQTLLATALDKMGPLFNLPFHLKTTA---SAKMWGFSTAHSNRFSSKLPWKETFSFGF 131
           G+ + L++ A +     F++P   K      S +  G++++ + RFS+KLPWKET SF F
Sbjct: 100 GISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLSFRF 159

Query: 132 DHCKTSNNEALVVDFFSSILGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGVERSHF 191
             C   +    V D+F   LGH FQ  G +Y++YCEAM  L L + ELLG+SLGV+R +F
Sbjct: 160 --CDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDYF 219

Query: 192 RKFFEDGSSIMRLNSYPICEQGSVALGTGPHCDPTGLTILHQDQVGGLEVFADNKWHTVP 251
           R+FFE+  SIMRLN YP C +  + LGTGPHCDPT LTILHQD V GL+VF +N+W ++ 
Sbjct: 220 REFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSIR 279

Query: 252 PSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPETLVV 311
           P+  A V+N+GD FMAL N  YKSC+HRA+VN   ER+SLAFFLCP+KD+VV PP  L+ 
Sbjct: 280 PNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRELLD 339

Query: 312 GGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWV 346
              SR+YPDF WS  LEFTQK+YRAD  TLQ F+ W+
Sbjct: 340 SITSRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDWL 373

BLAST of Csor.00g125720 vs. ExPASy Swiss-Prot
Match: O04706 (Gibberellin 20 oxidase 1-B OS=Triticum aestivum OX=4565 GN=GA20ox1B PE=2 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 1.8e-97
Identity = 172/339 (50.74%), Postives = 223/339 (65.78%), Query Frame = 0

Query: 12  LSSQFIWPK-EDLVDAYDEKLSEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVIN 71
           + SQFIWP+ E       E+L  P +D+ G L GD +AT E + LV  AC +HGFFQV+N
Sbjct: 17  IPSQFIWPEGESPTPDAAEELHVPLIDIGGMLSGDPRATAEVTRLVGEACERHGFFQVVN 76

Query: 72  HGVDQTLLATALDKMGPLFNLPFHLKTTA---SAKMWGFSTAHSNRFSSKLPWKETFSFG 131
           HG+D  LLA A   +   F +P   K  A     +  G++++ + RF+SKLPWKET SF 
Sbjct: 77  HGIDAELLADAHRCVDAFFTMPLPEKQRALRRPGESCGYASSFTGRFASKLPWKETLSFR 136

Query: 132 FDHCKTSNNEALVVDFFSSILGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGVERSH 191
                  ++ ALVVD+  + LG + + +G +Y +YC  M  L L + E+LG SLGV R+H
Sbjct: 137 ----SCPSDPALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESLGVGRAH 196

Query: 192 FRKFFEDGSSIMRLNSYPICEQGSVALGTGPHCDPTGLTILHQDQVGGLEVFADNKWHTV 251
           +R+FFE   SIMRLN YP C++    LGTGPHCDPT LTILHQD VGGL+V  + +W ++
Sbjct: 197 YRRFFEGNDSIMRLNYYPPCQRPMETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRWRSI 256

Query: 252 PPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPETLV 311
            P  +A V+N+GD FMAL NG YKSC+HRA+VN    R+SLAFFLCP  DKVV PP TLV
Sbjct: 257 RPRADAFVVNIGDTFMALSNGRYKSCLHRAVVNSKVPRKSLAFFLCPEMDKVVAPPGTLV 316

Query: 312 VGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVV 347
                R YPDF W  LL+FTQK+YRAD  TL+ F+ W+V
Sbjct: 317 DAANPRAYPDFTWRSLLDFTQKHYRADMKTLEVFSSWIV 351

BLAST of Csor.00g125720 vs. ExPASy Swiss-Prot
Match: P0C5H5 (Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. indica OX=39946 GN=20ox2 PE=1 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 8.9e-97
Identity = 174/333 (52.25%), Postives = 220/333 (66.07%), Query Frame = 0

Query: 16  FIWPKEDLVDAYDEKLSEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVINHGVDQ 75
           F+WP  D   A   +L  P VD+     GD +    A+  V  AC  HGFFQV  HGVD 
Sbjct: 46  FVWPNGDARPASAAELDMPVVDVGVLRDGDAEGLRRAAAQVAAACATHGFFQVSEHGVDA 105

Query: 76  TLLATALDKMGPLFNLPFHLKTTA---SAKMWGFSTAHSNRFSSKLPWKETFSFGFDHCK 135
            L   ALD     F LP   K  A      + G+++AH++RF+SKLPWKET SFGF H +
Sbjct: 106 ALARAALDGASDFFRLPLAEKRRARRVPGTVSGYTSAHADRFASKLPWKETLSFGF-HDR 165

Query: 136 TSNNEALVVDFFSSILGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGVERSHFRKFF 195
            +    +V D+FSS LG +F  +G +Y+KYCE M++L L + ELL +SLGVER ++R+FF
Sbjct: 166 AA--APVVADYFSSTLGPDFAPMGRVYQKYCEEMKELSLTIMELLELSLGVERGYYREFF 225

Query: 196 EDGSSIMRLNSYPICEQGSVALGTGPHCDPTGLTILHQDQVGGLEVFADNKWHTVPPSQN 255
            D SSIMR N YP C +    LGTGPHCDPT LTIL QD VGGLEV  D +W  V P   
Sbjct: 226 ADSSSIMRCNYYPPCPEPERTLGTGPHCDPTALTILLQDDVGGLEVLVDGEWRPVSPVPG 285

Query: 256 ALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPETLVVGGGS 315
           A+VIN+GD FMAL NG YKSC+HRA+VN+ +ERRSLAFFLCPR+D+VVRPP +       
Sbjct: 286 AMVINIGDTFMALSNGRYKSCLHRAVVNQRRERRSLAFFLCPREDRVVRPPPSAAT---P 345

Query: 316 RKYPDFAWSELLEFTQKYYRADAVTLQNFTKWV 346
           + YPDF W++L+ FTQ++YRAD  TL  FT+W+
Sbjct: 346 QHYPDFTWADLMRFTQRHYRADTRTLDAFTRWL 372

BLAST of Csor.00g125720 vs. NCBI nr
Match: KAG6578931.1 (Gibberellin 20 oxidase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 731 bits (1886), Expect = 2.81e-266
Identity = 353/353 (100.00%), Postives = 353/353 (100.00%), Query Frame = 0

Query: 1   MAMDSSSPLLVLSSQFIWPKEDLVDAYDEKLSEPHVDLHGFLKGDQKATIEASHLVRTAC 60
           MAMDSSSPLLVLSSQFIWPKEDLVDAYDEKLSEPHVDLHGFLKGDQKATIEASHLVRTAC
Sbjct: 1   MAMDSSSPLLVLSSQFIWPKEDLVDAYDEKLSEPHVDLHGFLKGDQKATIEASHLVRTAC 60

Query: 61  MKHGFFQVINHGVDQTLLATALDKMGPLFNLPFHLKTTASAKMWGFSTAHSNRFSSKLPW 120
           MKHGFFQVINHGVDQTLLATALDKMGPLFNLPFHLKTTASAKMWGFSTAHSNRFSSKLPW
Sbjct: 61  MKHGFFQVINHGVDQTLLATALDKMGPLFNLPFHLKTTASAKMWGFSTAHSNRFSSKLPW 120

Query: 121 KETFSFGFDHCKTSNNEALVVDFFSSILGHEFQEIGALYEKYCEAMRDLGLALTELLGIS 180
           KETFSFGFDHCKTSNNEALVVDFFSSILGHEFQEIGALYEKYCEAMRDLGLALTELLGIS
Sbjct: 121 KETFSFGFDHCKTSNNEALVVDFFSSILGHEFQEIGALYEKYCEAMRDLGLALTELLGIS 180

Query: 181 LGVERSHFRKFFEDGSSIMRLNSYPICEQGSVALGTGPHCDPTGLTILHQDQVGGLEVFA 240
           LGVERSHFRKFFEDGSSIMRLNSYPICEQGSVALGTGPHCDPTGLTILHQDQVGGLEVFA
Sbjct: 181 LGVERSHFRKFFEDGSSIMRLNSYPICEQGSVALGTGPHCDPTGLTILHQDQVGGLEVFA 240

Query: 241 DNKWHTVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVV 300
           DNKWHTVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVV
Sbjct: 241 DNKWHTVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVV 300

Query: 301 RPPETLVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARPCDV 353
           RPPETLVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARPCDV
Sbjct: 301 RPPETLVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARPCDV 353

BLAST of Csor.00g125720 vs. NCBI nr
Match: XP_022939151.1 (gibberellin 20 oxidase 3-like [Cucurbita moschata] >KAG7016454.1 Gibberellin 20 oxidase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 725 bits (1872), Expect = 3.56e-264
Identity = 350/351 (99.72%), Postives = 350/351 (99.72%), Query Frame = 0

Query: 3   MDSSSPLLVLSSQFIWPKEDLVDAYDEKLSEPHVDLHGFLKGDQKATIEASHLVRTACMK 62
           MDSSSPLLVLSSQFIWPKEDLVDAYDEKLSEPHVDLHGFLKGDQKATIEASHLVRTACMK
Sbjct: 1   MDSSSPLLVLSSQFIWPKEDLVDAYDEKLSEPHVDLHGFLKGDQKATIEASHLVRTACMK 60

Query: 63  HGFFQVINHGVDQTLLATALDKMGPLFNLPFHLKTTASAKMWGFSTAHSNRFSSKLPWKE 122
           HGFFQVINHGVDQTLLATALDKMGPLFNLPFHLKTTASAKMWGFSTAHSNRFSSKLPWKE
Sbjct: 61  HGFFQVINHGVDQTLLATALDKMGPLFNLPFHLKTTASAKMWGFSTAHSNRFSSKLPWKE 120

Query: 123 TFSFGFDHCKTSNNEALVVDFFSSILGHEFQEIGALYEKYCEAMRDLGLALTELLGISLG 182
           TFSFGFDHCKTSNNE LVVDFFSSILGHEFQEIGALYEKYCEAMRDLGLALTELLGISLG
Sbjct: 121 TFSFGFDHCKTSNNEPLVVDFFSSILGHEFQEIGALYEKYCEAMRDLGLALTELLGISLG 180

Query: 183 VERSHFRKFFEDGSSIMRLNSYPICEQGSVALGTGPHCDPTGLTILHQDQVGGLEVFADN 242
           VERSHFRKFFEDGSSIMRLNSYPICEQGSVALGTGPHCDPTGLTILHQDQVGGLEVFADN
Sbjct: 181 VERSHFRKFFEDGSSIMRLNSYPICEQGSVALGTGPHCDPTGLTILHQDQVGGLEVFADN 240

Query: 243 KWHTVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRP 302
           KWHTVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRP
Sbjct: 241 KWHTVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRP 300

Query: 303 PETLVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARPCDV 353
           PETLVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARPCDV
Sbjct: 301 PETLVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARPCDV 351

BLAST of Csor.00g125720 vs. NCBI nr
Match: XP_022993350.1 (gibberellin 20 oxidase 3-like [Cucurbita maxima])

HSP 1 Score: 718 bits (1854), Expect = 1.97e-261
Identity = 345/351 (98.29%), Postives = 349/351 (99.43%), Query Frame = 0

Query: 3   MDSSSPLLVLSSQFIWPKEDLVDAYDEKLSEPHVDLHGFLKGDQKATIEASHLVRTACMK 62
           MDSSSPLLVLSSQFIWPKEDLVDAYD+KL+EPHVDLHGFLKGDQKATIEASHLVRTACMK
Sbjct: 1   MDSSSPLLVLSSQFIWPKEDLVDAYDQKLTEPHVDLHGFLKGDQKATIEASHLVRTACMK 60

Query: 63  HGFFQVINHGVDQTLLATALDKMGPLFNLPFHLKTTASAKMWGFSTAHSNRFSSKLPWKE 122
           HGFFQVINHGVDQTLLATALDKMGPLFNLPFHLKTTASAKMWGFSTAHSNRFSSKLPWKE
Sbjct: 61  HGFFQVINHGVDQTLLATALDKMGPLFNLPFHLKTTASAKMWGFSTAHSNRFSSKLPWKE 120

Query: 123 TFSFGFDHCKTSNNEALVVDFFSSILGHEFQEIGALYEKYCEAMRDLGLALTELLGISLG 182
           TFSFGFDHCKTSNNE LVVDFFSS+LGHEFQEIGALYEKYCEAMRDLGLALTELLGISLG
Sbjct: 121 TFSFGFDHCKTSNNEPLVVDFFSSMLGHEFQEIGALYEKYCEAMRDLGLALTELLGISLG 180

Query: 183 VERSHFRKFFEDGSSIMRLNSYPICEQGSVALGTGPHCDPTGLTILHQDQVGGLEVFADN 242
           VERSHFRKFFEDGSSIMRLNSYPICEQGS+ALGTGPHCDPTGLTILHQDQVGGLEVFADN
Sbjct: 181 VERSHFRKFFEDGSSIMRLNSYPICEQGSLALGTGPHCDPTGLTILHQDQVGGLEVFADN 240

Query: 243 KWHTVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRP 302
           KWHTVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRP
Sbjct: 241 KWHTVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRP 300

Query: 303 PETLVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARPCDV 353
           PE LVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARPCDV
Sbjct: 301 PEKLVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARPCDV 351

BLAST of Csor.00g125720 vs. NCBI nr
Match: XP_023550661.1 (gibberellin 20 oxidase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 707 bits (1825), Expect = 5.01e-257
Identity = 340/350 (97.14%), Postives = 346/350 (98.86%), Query Frame = 0

Query: 3   MDSSSPLLVLSSQFIWPKEDLVDAYDEKLSEPHVDLHGFLKGDQKATIEASHLVRTACMK 62
           MDSSSPLLVLSSQFIWPKEDLVDAY EKLSEPHVDLHGFLKGDQKATIEASH+VRTACMK
Sbjct: 1   MDSSSPLLVLSSQFIWPKEDLVDAYYEKLSEPHVDLHGFLKGDQKATIEASHIVRTACMK 60

Query: 63  HGFFQVINHGVDQTLLATALDKMGPLFNLPFHLKTTASAKMWGFSTAHSNRFSSKLPWKE 122
           HGFFQVINHGVDQ+LLATALDKMGPLFNLPF+LKTTAS KMWGFSTAHSNRFSSKLPWKE
Sbjct: 61  HGFFQVINHGVDQSLLATALDKMGPLFNLPFNLKTTASTKMWGFSTAHSNRFSSKLPWKE 120

Query: 123 TFSFGFDHCKTSNNEALVVDFFSSILGHEFQEIGALYEKYCEAMRDLGLALTELLGISLG 182
           TFSFGFDHCK+SNNE LVVDFFSS+LGHEFQEIGALYEKYCEAMRDLGLALTELLGISLG
Sbjct: 121 TFSFGFDHCKSSNNEPLVVDFFSSMLGHEFQEIGALYEKYCEAMRDLGLALTELLGISLG 180

Query: 183 VERSHFRKFFEDGSSIMRLNSYPICEQGSVALGTGPHCDPTGLTILHQDQVGGLEVFADN 242
           VERSHFRKFFEDGSSIMRLNSYPICEQGSVALGTGPHCDPTGLTILHQDQVGGLEVFADN
Sbjct: 181 VERSHFRKFFEDGSSIMRLNSYPICEQGSVALGTGPHCDPTGLTILHQDQVGGLEVFADN 240

Query: 243 KWHTVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRP 302
           KWHTVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRP
Sbjct: 241 KWHTVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRP 300

Query: 303 PETLVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARPCD 352
           PE LVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARPC+
Sbjct: 301 PEKLVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARPCE 350

BLAST of Csor.00g125720 vs. NCBI nr
Match: XP_004144295.2 (gibberellin 20 oxidase 1-D [Cucumis sativus] >KGN47512.1 hypothetical protein Csa_018977 [Cucumis sativus] >CCG14216.1 GA 20-oxidase [Cucumis sativus])

HSP 1 Score: 594 bits (1531), Expect = 8.92e-212
Identity = 284/346 (82.08%), Postives = 312/346 (90.17%), Query Frame = 0

Query: 10  LVLSSQFIWPKEDLVDAYDEKLSEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVI 69
           L + S+FIWPK DLV+AY EKLSEPHVDL GFLKGD+KAT+EAS LVR AC+KHGFFQV 
Sbjct: 32  LFVQSKFIWPKGDLVEAY-EKLSEPHVDLEGFLKGDKKATLEASKLVRKACLKHGFFQVT 91

Query: 70  NHGVDQTLLATALDKMGPLFNLPFHLKTTAS----AKMWGFSTAHSNRFSSKLPWKETFS 129
           NHGVDQ LLATAL +MGP+FNLPF++KT AS    AKMWGFSTAHSNRFSSKLPWKETFS
Sbjct: 92  NHGVDQNLLATALHEMGPIFNLPFNVKTRASQSHPAKMWGFSTAHSNRFSSKLPWKETFS 151

Query: 130 FGFDHCKTSNNEALVVDFFSSILGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGVER 189
           FGFDHC + NNE  VVDFFSS LG EF+EIG +YEKYCEAMRDL LALTELLGISLG+ER
Sbjct: 152 FGFDHCNSFNNEPSVVDFFSSTLGKEFKEIGVIYEKYCEAMRDLSLALTELLGISLGLER 211

Query: 190 SHFRKFFEDGSSIMRLNSYPICEQGSVALGTGPHCDPTGLTILHQDQVGGLEVFADNKWH 249
           SHFRKFFEDGSSIMRLNSYPICEQG VALGTGPHCDPT LTILHQDQVGGLEVFA+N+WH
Sbjct: 212 SHFRKFFEDGSSIMRLNSYPICEQGGVALGTGPHCDPTALTILHQDQVGGLEVFANNQWH 271

Query: 250 TVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPET 309
           +VPP+ NALV+N+GD+FMA CNGEYKSCVHRA+VN +K+RRSLAFFLCPRK+KVVRPPE 
Sbjct: 272 SVPPTPNALVVNIGDLFMAQCNGEYKSCVHRAVVNNYKKRRSLAFFLCPRKNKVVRPPEK 331

Query: 310 LVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARPC 351
           LV    SRKYPDF+WSELLEFTQK+YRADA TLQNFTKWVVS+RPC
Sbjct: 332 LVADDESRKYPDFSWSELLEFTQKHYRADAATLQNFTKWVVSSRPC 376

BLAST of Csor.00g125720 vs. ExPASy TrEMBL
Match: A0A6J1FF43 (gibberellin 20 oxidase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111445146 PE=3 SV=1)

HSP 1 Score: 725 bits (1872), Expect = 1.72e-264
Identity = 350/351 (99.72%), Postives = 350/351 (99.72%), Query Frame = 0

Query: 3   MDSSSPLLVLSSQFIWPKEDLVDAYDEKLSEPHVDLHGFLKGDQKATIEASHLVRTACMK 62
           MDSSSPLLVLSSQFIWPKEDLVDAYDEKLSEPHVDLHGFLKGDQKATIEASHLVRTACMK
Sbjct: 1   MDSSSPLLVLSSQFIWPKEDLVDAYDEKLSEPHVDLHGFLKGDQKATIEASHLVRTACMK 60

Query: 63  HGFFQVINHGVDQTLLATALDKMGPLFNLPFHLKTTASAKMWGFSTAHSNRFSSKLPWKE 122
           HGFFQVINHGVDQTLLATALDKMGPLFNLPFHLKTTASAKMWGFSTAHSNRFSSKLPWKE
Sbjct: 61  HGFFQVINHGVDQTLLATALDKMGPLFNLPFHLKTTASAKMWGFSTAHSNRFSSKLPWKE 120

Query: 123 TFSFGFDHCKTSNNEALVVDFFSSILGHEFQEIGALYEKYCEAMRDLGLALTELLGISLG 182
           TFSFGFDHCKTSNNE LVVDFFSSILGHEFQEIGALYEKYCEAMRDLGLALTELLGISLG
Sbjct: 121 TFSFGFDHCKTSNNEPLVVDFFSSILGHEFQEIGALYEKYCEAMRDLGLALTELLGISLG 180

Query: 183 VERSHFRKFFEDGSSIMRLNSYPICEQGSVALGTGPHCDPTGLTILHQDQVGGLEVFADN 242
           VERSHFRKFFEDGSSIMRLNSYPICEQGSVALGTGPHCDPTGLTILHQDQVGGLEVFADN
Sbjct: 181 VERSHFRKFFEDGSSIMRLNSYPICEQGSVALGTGPHCDPTGLTILHQDQVGGLEVFADN 240

Query: 243 KWHTVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRP 302
           KWHTVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRP
Sbjct: 241 KWHTVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRP 300

Query: 303 PETLVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARPCDV 353
           PETLVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARPCDV
Sbjct: 301 PETLVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARPCDV 351

BLAST of Csor.00g125720 vs. ExPASy TrEMBL
Match: A0A6J1JYA4 (gibberellin 20 oxidase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111489390 PE=3 SV=1)

HSP 1 Score: 718 bits (1854), Expect = 9.56e-262
Identity = 345/351 (98.29%), Postives = 349/351 (99.43%), Query Frame = 0

Query: 3   MDSSSPLLVLSSQFIWPKEDLVDAYDEKLSEPHVDLHGFLKGDQKATIEASHLVRTACMK 62
           MDSSSPLLVLSSQFIWPKEDLVDAYD+KL+EPHVDLHGFLKGDQKATIEASHLVRTACMK
Sbjct: 1   MDSSSPLLVLSSQFIWPKEDLVDAYDQKLTEPHVDLHGFLKGDQKATIEASHLVRTACMK 60

Query: 63  HGFFQVINHGVDQTLLATALDKMGPLFNLPFHLKTTASAKMWGFSTAHSNRFSSKLPWKE 122
           HGFFQVINHGVDQTLLATALDKMGPLFNLPFHLKTTASAKMWGFSTAHSNRFSSKLPWKE
Sbjct: 61  HGFFQVINHGVDQTLLATALDKMGPLFNLPFHLKTTASAKMWGFSTAHSNRFSSKLPWKE 120

Query: 123 TFSFGFDHCKTSNNEALVVDFFSSILGHEFQEIGALYEKYCEAMRDLGLALTELLGISLG 182
           TFSFGFDHCKTSNNE LVVDFFSS+LGHEFQEIGALYEKYCEAMRDLGLALTELLGISLG
Sbjct: 121 TFSFGFDHCKTSNNEPLVVDFFSSMLGHEFQEIGALYEKYCEAMRDLGLALTELLGISLG 180

Query: 183 VERSHFRKFFEDGSSIMRLNSYPICEQGSVALGTGPHCDPTGLTILHQDQVGGLEVFADN 242
           VERSHFRKFFEDGSSIMRLNSYPICEQGS+ALGTGPHCDPTGLTILHQDQVGGLEVFADN
Sbjct: 181 VERSHFRKFFEDGSSIMRLNSYPICEQGSLALGTGPHCDPTGLTILHQDQVGGLEVFADN 240

Query: 243 KWHTVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRP 302
           KWHTVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRP
Sbjct: 241 KWHTVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRP 300

Query: 303 PETLVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARPCDV 353
           PE LVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARPCDV
Sbjct: 301 PEKLVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARPCDV 351

BLAST of Csor.00g125720 vs. ExPASy TrEMBL
Match: S0BE44 (GA 20-oxidase OS=Cucumis sativus OX=3659 GN=GA20ox5 PE=2 SV=1)

HSP 1 Score: 594 bits (1531), Expect = 4.32e-212
Identity = 284/346 (82.08%), Postives = 312/346 (90.17%), Query Frame = 0

Query: 10  LVLSSQFIWPKEDLVDAYDEKLSEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVI 69
           L + S+FIWPK DLV+AY EKLSEPHVDL GFLKGD+KAT+EAS LVR AC+KHGFFQV 
Sbjct: 32  LFVQSKFIWPKGDLVEAY-EKLSEPHVDLEGFLKGDKKATLEASKLVRKACLKHGFFQVT 91

Query: 70  NHGVDQTLLATALDKMGPLFNLPFHLKTTAS----AKMWGFSTAHSNRFSSKLPWKETFS 129
           NHGVDQ LLATAL +MGP+FNLPF++KT AS    AKMWGFSTAHSNRFSSKLPWKETFS
Sbjct: 92  NHGVDQNLLATALHEMGPIFNLPFNVKTRASQSHPAKMWGFSTAHSNRFSSKLPWKETFS 151

Query: 130 FGFDHCKTSNNEALVVDFFSSILGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGVER 189
           FGFDHC + NNE  VVDFFSS LG EF+EIG +YEKYCEAMRDL LALTELLGISLG+ER
Sbjct: 152 FGFDHCNSFNNEPSVVDFFSSTLGKEFKEIGVIYEKYCEAMRDLSLALTELLGISLGLER 211

Query: 190 SHFRKFFEDGSSIMRLNSYPICEQGSVALGTGPHCDPTGLTILHQDQVGGLEVFADNKWH 249
           SHFRKFFEDGSSIMRLNSYPICEQG VALGTGPHCDPT LTILHQDQVGGLEVFA+N+WH
Sbjct: 212 SHFRKFFEDGSSIMRLNSYPICEQGGVALGTGPHCDPTALTILHQDQVGGLEVFANNQWH 271

Query: 250 TVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPET 309
           +VPP+ NALV+N+GD+FMA CNGEYKSCVHRA+VN +K+RRSLAFFLCPRK+KVVRPPE 
Sbjct: 272 SVPPTPNALVVNIGDLFMAQCNGEYKSCVHRAVVNNYKKRRSLAFFLCPRKNKVVRPPEK 331

Query: 310 LVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARPC 351
           LV    SRKYPDF+WSELLEFTQK+YRADA TLQNFTKWVVS+RPC
Sbjct: 332 LVADDESRKYPDFSWSELLEFTQKHYRADAATLQNFTKWVVSSRPC 376

BLAST of Csor.00g125720 vs. ExPASy TrEMBL
Match: A0A5D3D529 (Gibberellin 20 oxidase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold390G00180 PE=3 SV=1)

HSP 1 Score: 581 bits (1497), Expect = 6.50e-207
Identity = 279/345 (80.87%), Postives = 308/345 (89.28%), Query Frame = 0

Query: 10  LVLSSQFIWPKEDLVDAYDEKLSEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVI 69
           L + S+FIWPK D+V+AY  KLSEP+VDL GFLKGD+KAT+EAS LVR AC++HGFFQV 
Sbjct: 32  LFVPSKFIWPKGDIVEAYG-KLSEPYVDLEGFLKGDKKATLEASKLVRKACLEHGFFQVT 91

Query: 70  NHGVDQTLLATALDKMGPLFNLPFHLKTTAS----AKMWGFSTAHSNRFSSKLPWKETFS 129
           NHGVDQ LLATAL +MGP+FNLPF++KT AS    AKMWGFSTAHSNRFSSKLPWKETFS
Sbjct: 92  NHGVDQNLLATALLEMGPIFNLPFNVKTRASQSHPAKMWGFSTAHSNRFSSKLPWKETFS 151

Query: 130 FGFDHCKTSNNEALVVDFFSSILGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGVER 189
           FGFDHC + NNE  VVDFFSS LG EF+EIG +YEKYCEAMRDL LALTELLGISLG+ER
Sbjct: 152 FGFDHCNSFNNEPSVVDFFSSALGKEFKEIGVIYEKYCEAMRDLSLALTELLGISLGLER 211

Query: 190 SHFRKFFEDGSSIMRLNSYPICEQGSVALGTGPHCDPTGLTILHQDQVGGLEVFADNKWH 249
           SHFR FFEDGSSIMRLNSYPICEQG VALGTGPHCDPT LTILHQDQVGGLEVFA+N WH
Sbjct: 212 SHFRMFFEDGSSIMRLNSYPICEQGGVALGTGPHCDPTALTILHQDQVGGLEVFANNLWH 271

Query: 250 TVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPET 309
           +VPPS NALV+N+GD+ MA CNGEYKSCVHRA+VN+HK+RRSLAFFLCPRKDKVVRPPE 
Sbjct: 272 SVPPSPNALVVNIGDLLMAQCNGEYKSCVHRAVVNQHKKRRSLAFFLCPRKDKVVRPPEK 331

Query: 310 LVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARP 350
           L+    SRKYPDF+WSELLEFTQK+YRADA TLQNFTKWVVS+RP
Sbjct: 332 LISDDESRKYPDFSWSELLEFTQKHYRADAATLQNFTKWVVSSRP 375

BLAST of Csor.00g125720 vs. ExPASy TrEMBL
Match: A0A1S3B3S2 (gibberellin 20 oxidase 3 OS=Cucumis melo OX=3656 GN=LOC103485678 PE=3 SV=1)

HSP 1 Score: 581 bits (1497), Expect = 6.50e-207
Identity = 279/345 (80.87%), Postives = 308/345 (89.28%), Query Frame = 0

Query: 10  LVLSSQFIWPKEDLVDAYDEKLSEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVI 69
           L + S+FIWPK D+V+AY  KLSEP+VDL GFLKGD+KAT+EAS LVR AC++HGFFQV 
Sbjct: 32  LFVPSKFIWPKGDIVEAYG-KLSEPYVDLEGFLKGDKKATLEASKLVRKACLEHGFFQVT 91

Query: 70  NHGVDQTLLATALDKMGPLFNLPFHLKTTAS----AKMWGFSTAHSNRFSSKLPWKETFS 129
           NHGVDQ LLATAL +MGP+FNLPF++KT AS    AKMWGFSTAHSNRFSSKLPWKETFS
Sbjct: 92  NHGVDQNLLATALLEMGPIFNLPFNVKTRASQSHPAKMWGFSTAHSNRFSSKLPWKETFS 151

Query: 130 FGFDHCKTSNNEALVVDFFSSILGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGVER 189
           FGFDHC + NNE  VVDFFSS LG EF+EIG +YEKYCEAMRDL LALTELLGISLG+ER
Sbjct: 152 FGFDHCNSFNNEPSVVDFFSSALGKEFKEIGVIYEKYCEAMRDLSLALTELLGISLGLER 211

Query: 190 SHFRKFFEDGSSIMRLNSYPICEQGSVALGTGPHCDPTGLTILHQDQVGGLEVFADNKWH 249
           SHFR FFEDGSSIMRLNSYPICEQG VALGTGPHCDPT LTILHQDQVGGLEVFA+N WH
Sbjct: 212 SHFRMFFEDGSSIMRLNSYPICEQGGVALGTGPHCDPTALTILHQDQVGGLEVFANNLWH 271

Query: 250 TVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPET 309
           +VPPS NALV+N+GD+ MA CNGEYKSCVHRA+VN+HK+RRSLAFFLCPRKDKVVRPPE 
Sbjct: 272 SVPPSPNALVVNIGDLLMAQCNGEYKSCVHRAVVNQHKKRRSLAFFLCPRKDKVVRPPEK 331

Query: 310 LVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARP 350
           L+    SRKYPDF+WSELLEFTQK+YRADA TLQNFTKWVVS+RP
Sbjct: 332 LISDDESRKYPDFSWSELLEFTQKHYRADAATLQNFTKWVVSSRP 375

BLAST of Csor.00g125720 vs. TAIR 10
Match: AT5G07200.1 (gibberellin 20-oxidase 3 )

HSP 1 Score: 377.1 bits (967), Expect = 1.6e-104
Identity = 183/344 (53.20%), Postives = 234/344 (68.02%), Query Frame = 0

Query: 12  LSSQFIWPKEDLVDAYDEKLSEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVINH 71
           +  QF+WP  +      + L  P +DL GFL GD     EA+ LV  A  KHGFF + NH
Sbjct: 37  IPQQFVWPDHEKPSTDVQPLQVPLIDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNH 96

Query: 72  GVDQTLLATALDKMGPLFNLPFHLKTTASAKMWGFSTAHSN----RFSSKLPWKETFSFG 131
           GVD++LL+ A   M   F  P   K  A  K WG S+ +++    RFSSKLPWKET SF 
Sbjct: 97  GVDESLLSRAYLHMDSFFKAPACEKQKAQRK-WGESSGYASSFVGRFSSKLPWKETLSFK 156

Query: 132 FDHCKTSNNEALVVDFFSSILGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGVERSH 191
           F   +  +++  V DF S  +G  +++ G +Y++Y EAM  L L + ELLG+SLGVER +
Sbjct: 157 FSPEEKIHSQT-VKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRY 216

Query: 192 FRKFFEDGSSIMRLNSYPICEQGSVALGTGPHCDPTGLTILHQDQVGGLEVFADNKWHTV 251
           F++FFED  SI RLN YP C+Q  +ALGTGPHCDPT LTILHQDQVGGL+VF DNKW ++
Sbjct: 217 FKEFFEDSDSIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSI 276

Query: 252 PPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPETLV 311
           PP+ +A V+N+GD FMAL NG YKSC+HRA+VN  +ER++ AFFLCP+ +KVV+PPE LV
Sbjct: 277 PPNPHAFVVNIGDTFMALTNGRYKSCLHRAVVNSERERKTFAFFLCPKGEKVVKPPEELV 336

Query: 312 VG--GGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSAR 350
            G   G RKYPDF WS  LEFTQK+YRAD  TL  F+ W+ + R
Sbjct: 337 NGVKSGERKYPDFTWSMFLEFTQKHYRADMNTLDEFSIWLKNRR 378

BLAST of Csor.00g125720 vs. TAIR 10
Match: AT5G51810.1 (gibberellin 20 oxidase 2 )

HSP 1 Score: 367.9 bits (943), Expect = 9.4e-102
Identity = 175/357 (49.02%), Postives = 237/357 (66.39%), Query Frame = 0

Query: 4   DSSSPLLV----------LSSQFIWPKEDLVDAYDEKLSEPHVDLHGFLKGDQKATIEAS 63
           D +SPL+           + +QFIWP E+       +L+ P +DL       Q +T+EA 
Sbjct: 24  DQTSPLIFNPSLLNLQSQIPNQFIWPDEEKPSIDIPELNVPFIDL-----SSQDSTLEAP 83

Query: 64  HLVRTACMKHGFFQVINHGVDQTLLATALDKMGPLFNLPFHLKTTASAK---MWGFSTAH 123
            ++  AC KHGFF V+NHGV ++L+A A   M   F++P   K  A  K     G++++ 
Sbjct: 84  RVIAEACTKHGFFLVVNHGVSESLIADAHRLMESFFDMPLAGKQKAQRKPGESCGYASSF 143

Query: 124 SNRFSSKLPWKETFSFGFDHCKTSNNEALVVDFFSSILGHEFQEIGALYEKYCEAMRDLG 183
           + RFS+KLPWKET SF F +   ++    V D+FS  LG EF++ G +Y+ YCEAM  L 
Sbjct: 144 TGRFSTKLPWKETLSFQFSN--DNSGSRTVQDYFSDTLGQEFEQFGKVYQDYCEAMSSLS 203

Query: 184 LALTELLGISLGVERSHFRKFFEDGSSIMRLNSYPICEQGSVALGTGPHCDPTGLTILHQ 243
           L + ELLG+SLGV R +FR FFE+  SIMRLN YP C+   + LGTGPHCDP+ LTILHQ
Sbjct: 204 LKIMELLGLSLGVNRDYFRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQ 263

Query: 244 DQVGGLEVFADNKWHTVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAF 303
           D V GL+VF DN+W ++ P+  A V+N+GD FMAL NG +KSC+HRA+VN+   R+S+AF
Sbjct: 264 DHVNGLQVFVDNQWQSIRPNPKAFVVNIGDTFMALSNGIFKSCLHRAVVNRESARKSMAF 323

Query: 304 FLCPRKDKVVRPPETLVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVS 348
           FLCP+KDKVV+PP  ++    +RKYPDF WS  LEFTQK+YRAD  TL +F+ WV++
Sbjct: 324 FLCPKKDKVVKPPSDILEKMKTRKYPDFTWSMFLEFTQKHYRADVNTLDSFSNWVIT 373

BLAST of Csor.00g125720 vs. TAIR 10
Match: AT4G25420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 365.5 bits (937), Expect = 4.7e-101
Identity = 171/337 (50.74%), Postives = 227/337 (67.36%), Query Frame = 0

Query: 12  LSSQFIWPKEDLVDAYDEKLSEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVINH 71
           + +QFIWP ++       +L  P +DL   L  D  +T++AS L+  AC KHGFF V+NH
Sbjct: 40  IPNQFIWPDDEKPSINVLELDVPLIDLQNLL-SDPSSTLDASRLISEACKKHGFFLVVNH 99

Query: 72  GVDQTLLATALDKMGPLFNLPFHLKTTA---SAKMWGFSTAHSNRFSSKLPWKETFSFGF 131
           G+ + L++ A +     F++P   K      S +  G++++ + RFS+KLPWKET SF F
Sbjct: 100 GISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLSFRF 159

Query: 132 DHCKTSNNEALVVDFFSSILGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGVERSHF 191
             C   +    V D+F   LGH FQ  G +Y++YCEAM  L L + ELLG+SLGV+R +F
Sbjct: 160 --CDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDYF 219

Query: 192 RKFFEDGSSIMRLNSYPICEQGSVALGTGPHCDPTGLTILHQDQVGGLEVFADNKWHTVP 251
           R+FFE+  SIMRLN YP C +  + LGTGPHCDPT LTILHQD V GL+VF +N+W ++ 
Sbjct: 220 REFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSIR 279

Query: 252 PSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPETLVV 311
           P+  A V+N+GD FMAL N  YKSC+HRA+VN   ER+SLAFFLCP+KD+VV PP  L+ 
Sbjct: 280 PNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRELLD 339

Query: 312 GGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWV 346
              SR+YPDF WS  LEFTQK+YRAD  TLQ F+ W+
Sbjct: 340 SITSRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDWL 373

BLAST of Csor.00g125720 vs. TAIR 10
Match: AT1G60980.1 (gibberellin 20-oxidase 4 )

HSP 1 Score: 351.3 bits (900), Expect = 9.1e-97
Identity = 169/338 (50.00%), Postives = 217/338 (64.20%), Query Frame = 0

Query: 12  LSSQFIWPKEDLVDAYDEKLSEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVINH 71
           +  +F+WP  +        L  P +DL GFL  D     EA  LV  A  KHGFF V NH
Sbjct: 36  IPQEFVWPDHEKPSKNVPILQVPVIDLAGFLSNDPLLVSEAERLVSEAAKKHGFFLVTNH 95

Query: 72  GVDQTLLATALDKMGPLFNLPFHLKTTASAKM---WGFSTAHSNRFSSKLPWKETFSFGF 131
           GVD+ LL+TA   M   F  P + K  A  K+    G++++   RF   LPWKET SF F
Sbjct: 96  GVDERLLSTAHKLMDTFFKSPNYEKLKAQRKVGETTGYASSFVGRFKENLPWKETLSFSF 155

Query: 132 DHCKTSNNEALVV-DFFSSILGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGVERSH 191
              + S N +  V ++ S  +G  +++ G++Y++Y E M +L L + ELLG+SLG++R H
Sbjct: 156 SPTEKSENYSQTVKNYISKTMGDGYKDFGSVYQEYAETMSNLSLKIMELLGMSLGIKREH 215

Query: 192 FRKFFEDGSSIMRLNSYPICEQGSVALGTGPHCDPTGLTILHQDQVGGLEVFADNKWHTV 251
           FR+FFED  SI RLN YP C+Q  + LGTGPHCDPT LTIL QDQV GL+VF DN+W ++
Sbjct: 216 FREFFEDNESIFRLNYYPKCKQPDLVLGTGPHCDPTSLTILQQDQVSGLQVFVDNQWQSI 275

Query: 252 PPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPETLV 311
           PP   ALV+N+GD  MAL NG YKSC+HRA+VN    R++LAFFLCP+ DKVV+PP  L 
Sbjct: 276 PPIPQALVVNIGDTLMALTNGIYKSCLHRAVVNGETTRKTLAFFLCPKVDKVVKPPSEL- 335

Query: 312 VGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWV 346
              G R YPDF WS  LEFT K+YRAD  TL+ FT W+
Sbjct: 336 --EGERAYPDFTWSMFLEFTMKHYRADMNTLEEFTNWL 370

BLAST of Csor.00g125720 vs. TAIR 10
Match: AT1G44090.1 (gibberellin 20-oxidase 5 )

HSP 1 Score: 328.9 bits (842), Expect = 4.9e-90
Identity = 167/339 (49.26%), Postives = 216/339 (63.72%), Query Frame = 0

Query: 14  SQFIWPKEDLVDAYDEKLSEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVINHGV 73
           ++F WP++D+  + +  L  P +DL GFL G++  T  A+  V+ ACM HG F V+NHG 
Sbjct: 45  AEFFWPEKDVAPS-EGDLDLPIIDLSGFLNGNEAETQLAAKAVKKACMAHGTFLVVNHGF 104

Query: 74  DQTLLATALDKMGPLFNLPFHLKTTA---SAKMWGFSTAHSNRFSSKLPWKETFSFGFDH 133
              L   AL+     F L    K  A      + G++  HS RFSS LPW ET +  F  
Sbjct: 105 KSGLAEKALEISSLFFGLSKDEKLRAYRIPGNISGYTAGHSQRFSSNLPWNETLTLAF-- 164

Query: 134 CKTSNNEALVVDFFSSILGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGV-ERSHFR 193
                   +V DF +S LG+  QEIG +++++C+AM  L + L ELLGIS+G+ +R+++R
Sbjct: 165 --KKGPPHVVEDFLTSRLGNHRQEIGQVFQEFCDAMNGLVMDLMELLGISMGLKDRTYYR 224

Query: 194 KFFEDGSSIMRLNSYPICEQGSVALGTGPHCDPTGLTILHQDQVGGLEVFADNKWHTVPP 253
           +FFEDGS I R N YP C+Q   ALG GPH DPT +T+L QD V GLEVFA   W TV P
Sbjct: 225 RFFEDGSGIFRCNYYPPCKQPEKALGVGPHNDPTAITVLLQDDVVGLEVFAAGSWQTVRP 284

Query: 254 SQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPETLVVG 313
              ALV+NVGD FMAL NG Y+SC HRA+VNK K RRSL FF CPR+DK++ PP  LV G
Sbjct: 285 RPGALVVNVGDTFMALSNGNYRSCYHRAVVNKEKVRRSLVFFSCPREDKIIVPPPELVEG 344

Query: 314 -GGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVS 348
              SRKYPDF W++L +FTQ  YR D  TL NF+ W+VS
Sbjct: 345 EEASRKYPDFTWAQLQKFTQSGYRVDNTTLHNFSSWLVS 378

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q391122.2e-10353.20Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=GA20OX3 PE=2 SV=1[more]
Q391111.3e-10049.02Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1[more]
Q391106.6e-10050.74Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2[more]
O047061.8e-9750.74Gibberellin 20 oxidase 1-B OS=Triticum aestivum OX=4565 GN=GA20ox1B PE=2 SV=1[more]
P0C5H58.9e-9752.25Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. indica OX=39946 GN=20ox2 PE=1 SV... [more]
Match NameE-valueIdentityDescription
KAG6578931.12.81e-266100.00Gibberellin 20 oxidase 3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022939151.13.56e-26499.72gibberellin 20 oxidase 3-like [Cucurbita moschata] >KAG7016454.1 Gibberellin 20 ... [more]
XP_022993350.11.97e-26198.29gibberellin 20 oxidase 3-like [Cucurbita maxima][more]
XP_023550661.15.01e-25797.14gibberellin 20 oxidase 3-like [Cucurbita pepo subsp. pepo][more]
XP_004144295.28.92e-21282.08gibberellin 20 oxidase 1-D [Cucumis sativus] >KGN47512.1 hypothetical protein Cs... [more]
Match NameE-valueIdentityDescription
A0A6J1FF431.72e-26499.72gibberellin 20 oxidase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111445146 PE=3... [more]
A0A6J1JYA49.56e-26298.29gibberellin 20 oxidase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111489390 PE=3 S... [more]
S0BE444.32e-21282.08GA 20-oxidase OS=Cucumis sativus OX=3659 GN=GA20ox5 PE=2 SV=1[more]
A0A5D3D5296.50e-20780.87Gibberellin 20 oxidase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3B3S26.50e-20780.87gibberellin 20 oxidase 3 OS=Cucumis melo OX=3656 GN=LOC103485678 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G07200.11.6e-10453.20gibberellin 20-oxidase 3 [more]
AT5G51810.19.4e-10249.02gibberellin 20 oxidase 2 [more]
AT4G25420.14.7e-10150.742-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G60980.19.1e-9750.00gibberellin 20-oxidase 4 [more]
AT1G44090.14.9e-9049.26gibberellin 20-oxidase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00682IPNSYNTHASEcoord: 56..73
score: 44.95
coord: 219..240
score: 36.36
coord: 282..300
score: 35.89
NoneNo IPR availablePANTHERPTHR47990:SF86GIBBERELLIN 20 OXIDASE 5coord: 11..347
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 11..347
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 13..333
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 34..130
e-value: 8.1E-15
score: 55.6
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 5..326
e-value: 6.1E-79
score: 267.9
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 197..294
e-value: 8.2E-26
score: 90.4
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 195..294
score: 12.743742

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g125720.m01Csor.00g125720.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009908 flower development
biological_process GO:0009686 gibberellin biosynthetic process
biological_process GO:0009416 response to light stimulus
biological_process GO:0009826 unidimensional cell growth
molecular_function GO:0045544 gibberellin 20-oxidase activity
molecular_function GO:0046872 metal ion binding