Csor.00g124560 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g124560
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionnifU-like protein 4, mitochondrial
LocationCsor_Chr15: 2475400 .. 2478490 (-)
RNA-Seq ExpressionCsor.00g124560
SyntenyCsor.00g124560
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGGGCTTAGGAAGTTTGATTGTGCGAGCTCGAAGAATTTCACCACTGGGTTCTCACGGAGTTGGGAAGATTCGATTCTTCTCAGCCGCAACAACTTCTTCTATATTCGGCGGAAAAGAAGCTGCTTCGTGTTCTGTATCGTCAAGAACTTGCTCCGATCCTCTCTTGCGTTCATGGAGATGGAGCCAATTTGGAGGTGCGTGAGCTTTGACGAACCATCATTGGAATCAGCTGTATTTGTTTTTGAATAATCTGTCGTATTTTGTTGCATTCAGGACAAAAAAGAACAATGTTCATTGAAACTCAGTCAACACCCAATCCTTCATCTCTCATGTTCTATCCTGGAAAGCCTGTTATGGAAGTCGGGAGTGCTGATTTTCCTAATGCACGTTCTGCTATGAACTCCCCACTAGCAAAGGCTCTTTATGGTGTTGACGGTACATATTCTGAACTTATGATATCTTTTTAGGTTTCTGTTGGATTTTCAATTTGGAATACCCGCTTCCTTTTATCTGAATTTTAAAGAGTTTAAGATGTATGCTTGCATTTTAGAGGTCTGGAATTATGTCGACATTGAACTGAGACATGGAACATTGAACTGAGAGTAGGAAAGTTGTGAGGCATAGTAAATAAGTGCTAGTTTGAAGTTTAATTGATATATGACTTGGTGTTGGTTTACAAGTGTTCTTACTGGTTTTACTGATTCCTGGCGTGGGGCGTTCTTGATTGCTTGTGCAGGTGTTATCCGAGTCTTCTTTGGATCAGATTTTGTTACTGTAACAAAGTCAAATGAGGCTTCGTGGGATTTCCTTAAGCCTGAAATTTTTGCTTCAATCATGGACTTCTATTCTTCTGGACAACCACTATTTCTAGACGCTAGTACTGCTGCTGCCATGGATACAGCCATCAACGAAGTATGTCTTCTAACTGCAAATTAATCCACTTTCTTGTTAGAGTTTTACTCATTAAAAAACAAGTTAATTTTTTAGATCACATGGTGTTCTCTTCTCGTCGAACTTTTACATATTGGTTAGAAATAAGAAAGTAGAAAACTCATTTACGTTTTTCTCTCTCTTGGGATATTTATGATAATTTTATATTAGGTTATGTTTTGATCACAAATTATGCAATTCTATATGGTTCTCTTTCTCGCTTTCGATACTCGACATGATATAATACTAGGTCAAATGTGAGTTCCTTGGTGAAATGAAACCACACTAATTGATATACATAGATTTTATCTTCCTTAAGTGCTAATATAGAACTATGAACATTTAATGTCATCATACGTCGGATTAAAAGTACTTGAGGCTCATGGTATTAATAGAGTAATGTGGTGCCTTGCAGTTCCATTTCTACTTTTTTGGATACAACTTTGGCTAAGAACTTGTTTTCTGATCTTCATATTGGTCATTTTGATGGACATTCGATTTTCTCTTAAGTCAAATTAGTTACATTTTTTCTGTCCTGTCGTTAAGAAACGGGTTTTTCCTTTAATATAAAGTGAGGGATATACAAGCTACTGTGCCACTTGTGGAGACCTAGAGAACTGAAAAGGAATTACACAAGATACTGTAGTTGTACTTTTTGTTTTCAATCACTGAAGTTCTCTTGTTAGCTGTTATTAAAATTAGTATGTCTACTCAAAGAAGTTGAGTTAAGTCCAGCGCTAGCAGCTATTGTCCTCTTTAGGCTTTCCCTTTCGGGCTTCCCAACAAGGTTTTTAAAACTCTTCTGCTAGGGTGAGGTTTCCACACCCTTAAGGGTGTTTCGTTTTCCCCCCCCAACCGATGTGGGATCTTACAAGTTACTTACCTAAAAGATGAGTATGTCAATGTCTTTCTATTATATGTCAAAATTTATTTGTAATATTTCAATTTGAGGTTTGACTTTGTGGTCGTTGTTTTCTGTGCTGCTAAGTATTGAATCTTTCAAAGTTATATTCGTCATGTTGGAATCTGAAACATCATAAATTTTTATTATTCTAGTAGTTGGAAACAGCTTGATTCTTAATAGATCTGTCCACTTGTGCCTTTATTTAGGATGATTCTGAAACTGTTGCTATGATTAAAGAGCTGCTTGAGACTCGTATTCGACCAACAGTGCAAGATGATGGTGGAGATATTGAATACAGGGGATATAATGAGTAGGTTTTTCAGTTACCGGCGTTCCGTAAAGCATTAGGAGTTGTATGAATGATTGCTTTTATATGGTTATATATTGAATTTTCCATTTCTATCTTCTTCAGAGAAACTGGAATTGTCAAATTGAGAATGCAAGGAGCATGCAGTGGCTGCCCAAGCTCATCCGTCACTCTAAAATCTGGCATTGAAAATATGCTGATGCATTATGTACCAGAGGTGAGAGTTATGTAATCTTTGTATTTGTAAGCTCATGTTCAATGAGAAGCATATCCAATATTCCACTTGGACATCTAGGTAGGTCCTCCATGCATATTGCTTTTGTTCCCTTCTTTTACATGATACCCGACCTGAGGTGTCCTACAGCCATATTTCTTAATTTAGTTGCATGATGTTAGTATTGAGCTTGAATTCACACTGACACCACCAGCTGGAATGGAAGGACTTTTTGTTTTTTTTAGATAATAGGAATGGCAGGACATTGCACTATATAATTGAGATTGAATCTAAGTTTTATGACTAAGAACAGTTGGTTTTCCAACTTCTCACTGAATCTTGCTATAAGCAAGTGGCTAGCTACCGTTCATGTTTTACTGAAAATAATCATATGAAATGCAACATGCTCGATGGATAACAAGAATGCATCGACGAACACCGCATGTTTGATAAGACGGAAGACAATGTACCCTAGAGAGCCAACGTAGAAAATCACTCACTTCAAAGGGAGAAAATTGAAATAGAATAATGTTACACAGCTGCTTAGTTCTAGTATATAGGTTCTTTCTTCCTCTTTTGTTTCTATGCTTCTCAGATCCATAAACATCTTTTTTTCTTTTTTTGGAATATCTATGATTTGAATGTCTATTCTCAATTTTTAGGTGAAGGGCGTGGAACAAGAATTTGACGTCGAGGAAGAGGATGTAAAGTCAACCAGCCAGATGGAGTAA

mRNA sequence

ATGAGGGGCTTAGGAAGTTTGATTGTGCGAGCTCGAAGAATTTCACCACTGGGTTCTCACGGAGTTGGGAAGATTCGATTCTTCTCAGCCGCAACAACTTCTTCTATATTCGGCGGAAAAGAAGCTGCTTCGTGTTCTGTATCGTCAAGAACTTGCTCCGATCCTCTCTTGCGTTCATGGAGATGGAGCCAATTTGGAGGACAAAAAAGAACAATGTTCATTGAAACTCAGTCAACACCCAATCCTTCATCTCTCATGTTCTATCCTGGAAAGCCTGTTATGGAAGTCGGGAGTGCTGATTTTCCTAATGCACGTTCTGCTATGAACTCCCCACTAGCAAAGGCTCTTTATGGTGTTGACGGTGTTATCCGAGTCTTCTTTGGATCAGATTTTGTTACTGTAACAAAGTCAAATGAGGCTTCGTGGGATTTCCTTAAGCCTGAAATTTTTGCTTCAATCATGGACTTCTATTCTTCTGGACAACCACTATTTCTAGACGCTAGTACTGCTGCTGCCATGGATACAGCCATCAACGAAGATGATTCTGAAACTGTTGCTATGATTAAAGAGCTGCTTGAGACTCGTATTCGACCAACAGTGCAAGATGATGGTGGAGATATTGAATACAGGGGATATAATGAAGAAACTGGAATTGTCAAATTGAGAATGCAAGGAGCATGCAGTGGCTGCCCAAGCTCATCCGTCACTCTAAAATCTGGCATTGAAAATATGCTGATGCATTATGTACCAGAGGTGAAGGGCGTGGAACAAGAATTTGACGTCGAGGAAGAGGATGTAAAGTCAACCAGCCAGATGGAGTAA

Coding sequence (CDS)

ATGAGGGGCTTAGGAAGTTTGATTGTGCGAGCTCGAAGAATTTCACCACTGGGTTCTCACGGAGTTGGGAAGATTCGATTCTTCTCAGCCGCAACAACTTCTTCTATATTCGGCGGAAAAGAAGCTGCTTCGTGTTCTGTATCGTCAAGAACTTGCTCCGATCCTCTCTTGCGTTCATGGAGATGGAGCCAATTTGGAGGACAAAAAAGAACAATGTTCATTGAAACTCAGTCAACACCCAATCCTTCATCTCTCATGTTCTATCCTGGAAAGCCTGTTATGGAAGTCGGGAGTGCTGATTTTCCTAATGCACGTTCTGCTATGAACTCCCCACTAGCAAAGGCTCTTTATGGTGTTGACGGTGTTATCCGAGTCTTCTTTGGATCAGATTTTGTTACTGTAACAAAGTCAAATGAGGCTTCGTGGGATTTCCTTAAGCCTGAAATTTTTGCTTCAATCATGGACTTCTATTCTTCTGGACAACCACTATTTCTAGACGCTAGTACTGCTGCTGCCATGGATACAGCCATCAACGAAGATGATTCTGAAACTGTTGCTATGATTAAAGAGCTGCTTGAGACTCGTATTCGACCAACAGTGCAAGATGATGGTGGAGATATTGAATACAGGGGATATAATGAAGAAACTGGAATTGTCAAATTGAGAATGCAAGGAGCATGCAGTGGCTGCCCAAGCTCATCCGTCACTCTAAAATCTGGCATTGAAAATATGCTGATGCATTATGTACCAGAGGTGAAGGGCGTGGAACAAGAATTTGACGTCGAGGAAGAGGATGTAAAGTCAACCAGCCAGATGGAGTAA

Protein sequence

MRGLGSLIVRARRISPLGSHGVGKIRFFSAATTSSIFGGKEAASCSVSSRTCSDPLLRSWRWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVDGVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDASTAAAMDTAINEDDSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVEQEFDVEEEDVKSTSQME
Homology
BLAST of Csor.00g124560 vs. ExPASy Swiss-Prot
Match: Q9LIG6 (NifU-like protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFU4 PE=2 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 6.9e-97
Identity = 186/282 (65.96%), Postives = 223/282 (79.08%), Query Frame = 0

Query: 1   MRGLGSLIVRARRISPLGSHGVGKIRFFSAATTSSIFGGKE----AASCSVSSRTCSDPL 60
           M+G+  L+    RI   G   V      +++++SS+   +     +A+  ++SRT    L
Sbjct: 1   MKGIARLVTSLSRIG--GRKVVSGTSTVTSSSSSSLLLSRRSLFISATNLLNSRTKDSAL 60

Query: 61  ------LRSWRWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNS 120
                 L + +W+  GGQ+RTMFI+TQSTPNPSSLMFYPGKPVMEVGSADFPN RSA+ S
Sbjct: 61  PSLNSSLLAQKWNFLGGQRRTMFIQTQSTPNPSSLMFYPGKPVMEVGSADFPNVRSALGS 120

Query: 121 PLAKALYGVDGVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDASTA 180
           PLAK++Y +DGV+RVFFGSDFVTVTKS++ SWD LKPEIFA++MDFYSSGQPLFLD+  A
Sbjct: 121 PLAKSIYSIDGVVRVFFGSDFVTVTKSDDVSWDILKPEIFAAVMDFYSSGQPLFLDSQAA 180

Query: 181 AAMDTAINEDDSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGC 240
           AA DTAI+EDDSETVAMIKELLETRIRP VQDDGGDIEY G++ E+GIVKLRMQGACSGC
Sbjct: 181 AAKDTAISEDDSETVAMIKELLETRIRPAVQDDGGDIEYCGFDPESGIVKLRMQGACSGC 240

Query: 241 PSSSVTLKSGIENMLMHYVPEVKGVEQEFDVEEEDVKSTSQM 273
           PSSSVTLKSGIENMLMHYV EVKGVEQEFD E+E+   + +M
Sbjct: 241 PSSSVTLKSGIENMLMHYVSEVKGVEQEFDGEDEEGTLSGEM 280

BLAST of Csor.00g124560 vs. ExPASy Swiss-Prot
Match: Q9C8J2 (NifU-like protein 5, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFU5 PE=2 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 3.5e-93
Identity = 170/221 (76.92%), Postives = 193/221 (87.33%), Query Frame = 0

Query: 48  SSRTCSDPLLRSWRWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSA 107
           +SR CS      + W +   Q+RTMFI+TQSTPNPSSLMF PGKPVME+GSADFPN+RSA
Sbjct: 54  ASRNCSRSSSFPFNWIE---QRRTMFIQTQSTPNPSSLMFSPGKPVMEIGSADFPNSRSA 113

Query: 108 MNSPLAKALYGVDGVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDA 167
           M+SPLAKA++ +DGV+RVF+GSDFVTVTKS++ +WD LKP+IFA +MDFYSSGQPLFLD+
Sbjct: 114 MSSPLAKAIFAIDGVVRVFYGSDFVTVTKSDDVTWDILKPDIFAVVMDFYSSGQPLFLDS 173

Query: 168 STAAAMDTAINEDDSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGAC 227
              AA DTAI+EDDSETVAMIKELLETRIRP+VQDDGGDIEY G++ ETGIVKLRMQGAC
Sbjct: 174 QATAAKDTAIHEDDSETVAMIKELLETRIRPSVQDDGGDIEYCGFDTETGIVKLRMQGAC 233

Query: 228 SGCPSSSVTLKSGIENMLMHYVPEVKGVEQEFDVEEEDVKS 269
           SGCPSSSVTLKSGIENMLMHYV EVKGVEQEFD EEE   S
Sbjct: 234 SGCPSSSVTLKSGIENMLMHYVSEVKGVEQEFDGEEEGTSS 271

BLAST of Csor.00g124560 vs. ExPASy Swiss-Prot
Match: Q8SY96 (NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila melanogaster OX=7227 GN=CG32500 PE=2 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.0e-60
Identity = 120/201 (59.70%), Postives = 150/201 (74.63%), Query Frame = 0

Query: 69  KRTMFIETQSTPNPSSLMFYPGKPVMEVGSA-DFPNARSAMNSPLAKALYGVDGVIRVFF 128
           +R+MFI+TQ TPNP SL F PG  V+  G+  DFPN  +A NSPLAK L+ V+GV  VFF
Sbjct: 64  RRSMFIQTQDTPNPESLKFLPGVDVLGKGNTYDFPNGTTAHNSPLAKLLFRVEGVKGVFF 123

Query: 129 GSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDASTAAAMDTAINEDDSETVAM 188
           G+DFVT++K   A W  +KPE+FA IMDF++SG P+  DA   A  DT I EDD ETV M
Sbjct: 124 GADFVTISKQEGAEWSLIKPEVFAVIMDFFASGLPVLNDAQPNA--DTEILEDDDETVMM 183

Query: 189 IKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSGIENMLMH 248
           IKELL+TRIRPTVQ+DGGDI + GY  E G+VKL+MQG+CS CPSS VTLK+G++NML  
Sbjct: 184 IKELLDTRIRPTVQEDGGDIVFMGY--EGGVVKLKMQGSCSSCPSSIVTLKNGVQNMLQF 243

Query: 249 YVPEVKGVEQEFDVEEEDVKS 269
           Y+PEV+ VEQ FD  +  ++S
Sbjct: 244 YIPEVESVEQVFDEADRMIES 260

BLAST of Csor.00g124560 vs. ExPASy Swiss-Prot
Match: Q9QZ23 (NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Mus musculus OX=10090 GN=Nfu1 PE=1 SV=2)

HSP 1 Score: 234.2 bits (596), Expect = 1.8e-60
Identity = 117/202 (57.92%), Postives = 151/202 (74.75%), Query Frame = 0

Query: 70  RTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVDGVIRVFFGS 129
           R MFI+TQ TPNP+SL F PGKPV+E  + DFP   +A  SPLA+ L+ ++GV  VFFG 
Sbjct: 55  RFMFIQTQDTPNPNSLKFIPGKPVLETRTMDFPTPAAAFRSPLARQLFRIEGVKSVFFGP 114

Query: 130 DFVTVTKSN-EASWDFLKPEIFASIMDFYSSGQPLFLDASTAAAMDTAINEDDSETVAMI 189
           DF+TVTK N E  W+ LKP+I+A+IMDF++SG PL  +  T      A +E+D E VAMI
Sbjct: 115 DFITVTKENEELDWNLLKPDIYATIMDFFASGLPLVTE-ETPPPPGEAGSEEDDEVVAMI 174

Query: 190 KELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHY 249
           KELL+TRIRPTVQ+DGGD+ YRG+  E GIV+L++QG+C+ CPSS +TLKSGI+NML  Y
Sbjct: 175 KELLDTRIRPTVQEDGGDVIYRGF--EDGIVRLKLQGSCTSCPSSIITLKSGIQNMLQFY 234

Query: 250 VPEVKGVEQEFDVEEEDVKSTS 271
           +PEV+GVEQ  D +E D K  +
Sbjct: 235 IPEVEGVEQVMDDDESDEKEAN 253

BLAST of Csor.00g124560 vs. ExPASy Swiss-Prot
Match: B4PZ52 (NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila yakuba OX=7245 GN=GE15286 PE=3 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 3.0e-60
Identity = 120/201 (59.70%), Postives = 149/201 (74.13%), Query Frame = 0

Query: 69  KRTMFIETQSTPNPSSLMFYPGKPVMEVGSA-DFPNARSAMNSPLAKALYGVDGVIRVFF 128
           +R+MFI+TQ TPNP SL F PG  V+  G+  DFPN  +A +SPLAK L+ V+GV  VFF
Sbjct: 64  RRSMFIQTQDTPNPDSLKFLPGVDVLGKGNTYDFPNGTTAHSSPLAKLLFRVEGVKGVFF 123

Query: 129 GSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDASTAAAMDTAINEDDSETVAM 188
           G+DFVT++K   A W  +KPE+FA IMDF++SG P+  DA   A  DT I EDD ETV M
Sbjct: 124 GADFVTISKQEGAEWSLIKPEVFAVIMDFFASGLPVLHDAQPNA--DTEILEDDDETVMM 183

Query: 189 IKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSGIENMLMH 248
           IKELL+TRIRPTVQ+DGGDI + GY  E G+VKL+MQG+CS CPSS VTLK+G++NML  
Sbjct: 184 IKELLDTRIRPTVQEDGGDIVFMGY--EAGVVKLKMQGSCSSCPSSIVTLKNGVQNMLQF 243

Query: 249 YVPEVKGVEQEFDVEEEDVKS 269
           Y+PEV+ VEQ FD  +  V S
Sbjct: 244 YIPEVESVEQVFDEADRMVDS 260

BLAST of Csor.00g124560 vs. NCBI nr
Match: KAG6578816.1 (NifU-like protein 4, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 536 bits (1380), Expect = 7.68e-192
Identity = 273/273 (100.00%), Postives = 273/273 (100.00%), Query Frame = 0

Query: 1   MRGLGSLIVRARRISPLGSHGVGKIRFFSAATTSSIFGGKEAASCSVSSRTCSDPLLRSW 60
           MRGLGSLIVRARRISPLGSHGVGKIRFFSAATTSSIFGGKEAASCSVSSRTCSDPLLRSW
Sbjct: 1   MRGLGSLIVRARRISPLGSHGVGKIRFFSAATTSSIFGGKEAASCSVSSRTCSDPLLRSW 60

Query: 61  RWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD 120
           RWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD
Sbjct: 61  RWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD 120

Query: 121 GVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDASTAAAMDTAINED 180
           GVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDASTAAAMDTAINED
Sbjct: 121 GVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDASTAAAMDTAINED 180

Query: 181 DSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSG 240
           DSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSG
Sbjct: 181 DSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSG 240

Query: 241 IENMLMHYVPEVKGVEQEFDVEEEDVKSTSQME 273
           IENMLMHYVPEVKGVEQEFDVEEEDVKSTSQME
Sbjct: 241 IENMLMHYVPEVKGVEQEFDVEEEDVKSTSQME 273

BLAST of Csor.00g124560 vs. NCBI nr
Match: XP_022939962.1 (nifU-like protein 4, mitochondrial [Cucurbita moschata])

HSP 1 Score: 530 bits (1366), Expect = 1.05e-189
Identity = 270/273 (98.90%), Postives = 271/273 (99.27%), Query Frame = 0

Query: 1   MRGLGSLIVRARRISPLGSHGVGKIRFFSAATTSSIFGGKEAASCSVSSRTCSDPLLRSW 60
           MRGLGSLIVRARRISPLGSHGVGKIRFFSAATTSSIFGG EAASCSVSSRTC DPLLRSW
Sbjct: 1   MRGLGSLIVRARRISPLGSHGVGKIRFFSAATTSSIFGGNEAASCSVSSRTCFDPLLRSW 60

Query: 61  RWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD 120
           RWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD
Sbjct: 61  RWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD 120

Query: 121 GVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDASTAAAMDTAINED 180
           GVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDA+TAAAMDTAINED
Sbjct: 121 GVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDANTAAAMDTAINED 180

Query: 181 DSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSG 240
           DSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSG
Sbjct: 181 DSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSG 240

Query: 241 IENMLMHYVPEVKGVEQEFDVEEEDVKSTSQME 273
           IENMLMHYVPEVKGVEQEFDVEEEDVKSTSQME
Sbjct: 241 IENMLMHYVPEVKGVEQEFDVEEEDVKSTSQME 273

BLAST of Csor.00g124560 vs. NCBI nr
Match: XP_022992592.1 (nifU-like protein 4, mitochondrial [Cucurbita maxima])

HSP 1 Score: 524 bits (1349), Expect = 4.08e-187
Identity = 267/273 (97.80%), Postives = 269/273 (98.53%), Query Frame = 0

Query: 1   MRGLGSLIVRARRISPLGSHGVGKIRFFSAATTSSIFGGKEAASCSVSSRTCSDPLLRSW 60
           MRGLGSLIVRARRIS LGSHG GKIRFFSAATTSSIFGGKEAASCSVSSRTCSDP LRSW
Sbjct: 1   MRGLGSLIVRARRISTLGSHGGGKIRFFSAATTSSIFGGKEAASCSVSSRTCSDPFLRSW 60

Query: 61  RWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD 120
            WSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNAR+AMNSPLAKALYGVD
Sbjct: 61  SWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARTAMNSPLAKALYGVD 120

Query: 121 GVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDASTAAAMDTAINED 180
           GVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDA+TAAAMDTAINED
Sbjct: 121 GVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDANTAAAMDTAINED 180

Query: 181 DSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSG 240
           DSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSG
Sbjct: 181 DSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSG 240

Query: 241 IENMLMHYVPEVKGVEQEFDVEEEDVKSTSQME 273
           IENMLMHYVPEVKGVEQEFDVEEEDVKSTSQME
Sbjct: 241 IENMLMHYVPEVKGVEQEFDVEEEDVKSTSQME 273

BLAST of Csor.00g124560 vs. NCBI nr
Match: XP_023550903.1 (nifU-like protein 4, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 523 bits (1348), Expect = 5.80e-187
Identity = 267/273 (97.80%), Postives = 268/273 (98.17%), Query Frame = 0

Query: 1   MRGLGSLIVRARRISPLGSHGVGKIRFFSAATTSSIFGGKEAASCSVSSRTCSDPLLRSW 60
           MRGLGSLIVRARRIS LGSHG GKIRFFSAATTSSIFGGKEA SC VSSRTCSDP LRSW
Sbjct: 1   MRGLGSLIVRARRISTLGSHGGGKIRFFSAATTSSIFGGKEAVSCYVSSRTCSDPFLRSW 60

Query: 61  RWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD 120
           RWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD
Sbjct: 61  RWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD 120

Query: 121 GVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDASTAAAMDTAINED 180
           GVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDA+TAAAMDTAINED
Sbjct: 121 GVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDANTAAAMDTAINED 180

Query: 181 DSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSG 240
           DSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSG
Sbjct: 181 DSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSG 240

Query: 241 IENMLMHYVPEVKGVEQEFDVEEEDVKSTSQME 273
           IENMLMHYVPEVKGVEQEFDVEEEDVKSTSQME
Sbjct: 241 IENMLMHYVPEVKGVEQEFDVEEEDVKSTSQME 273

BLAST of Csor.00g124560 vs. NCBI nr
Match: KAG7016340.1 (NifU-like protein 4, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 499 bits (1286), Expect = 1.31e-177
Identity = 251/253 (99.21%), Postives = 253/253 (100.00%), Query Frame = 0

Query: 1   MRGLGSLIVRARRISPLGSHGVGKIRFFSAATTSSIFGGKEAASCSVSSRTCSDPLLRSW 60
           MRGLGSLIVRARRISPLGSHGVGKIRFFSAATTSSIFGGKEAASCSVSSRTCSDPLLRSW
Sbjct: 13  MRGLGSLIVRARRISPLGSHGVGKIRFFSAATTSSIFGGKEAASCSVSSRTCSDPLLRSW 72

Query: 61  RWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD 120
           RWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD
Sbjct: 73  RWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD 132

Query: 121 GVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDASTAAAMDTAINED 180
           GVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDA+TAAAMDTAINED
Sbjct: 133 GVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDANTAAAMDTAINED 192

Query: 181 DSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSG 240
           DSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSG
Sbjct: 193 DSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSG 252

Query: 241 IENMLMHYVPEVK 253
           IENMLMHYVPEV+
Sbjct: 253 IENMLMHYVPEVR 265

BLAST of Csor.00g124560 vs. ExPASy TrEMBL
Match: A0A6J1FIB0 (nifU-like protein 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111445668 PE=3 SV=1)

HSP 1 Score: 530 bits (1366), Expect = 5.06e-190
Identity = 270/273 (98.90%), Postives = 271/273 (99.27%), Query Frame = 0

Query: 1   MRGLGSLIVRARRISPLGSHGVGKIRFFSAATTSSIFGGKEAASCSVSSRTCSDPLLRSW 60
           MRGLGSLIVRARRISPLGSHGVGKIRFFSAATTSSIFGG EAASCSVSSRTC DPLLRSW
Sbjct: 1   MRGLGSLIVRARRISPLGSHGVGKIRFFSAATTSSIFGGNEAASCSVSSRTCFDPLLRSW 60

Query: 61  RWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD 120
           RWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD
Sbjct: 61  RWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD 120

Query: 121 GVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDASTAAAMDTAINED 180
           GVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDA+TAAAMDTAINED
Sbjct: 121 GVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDANTAAAMDTAINED 180

Query: 181 DSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSG 240
           DSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSG
Sbjct: 181 DSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSG 240

Query: 241 IENMLMHYVPEVKGVEQEFDVEEEDVKSTSQME 273
           IENMLMHYVPEVKGVEQEFDVEEEDVKSTSQME
Sbjct: 241 IENMLMHYVPEVKGVEQEFDVEEEDVKSTSQME 273

BLAST of Csor.00g124560 vs. ExPASy TrEMBL
Match: A0A6J1JQB1 (nifU-like protein 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111488894 PE=3 SV=1)

HSP 1 Score: 524 bits (1349), Expect = 1.98e-187
Identity = 267/273 (97.80%), Postives = 269/273 (98.53%), Query Frame = 0

Query: 1   MRGLGSLIVRARRISPLGSHGVGKIRFFSAATTSSIFGGKEAASCSVSSRTCSDPLLRSW 60
           MRGLGSLIVRARRIS LGSHG GKIRFFSAATTSSIFGGKEAASCSVSSRTCSDP LRSW
Sbjct: 1   MRGLGSLIVRARRISTLGSHGGGKIRFFSAATTSSIFGGKEAASCSVSSRTCSDPFLRSW 60

Query: 61  RWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD 120
            WSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNAR+AMNSPLAKALYGVD
Sbjct: 61  SWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARTAMNSPLAKALYGVD 120

Query: 121 GVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDASTAAAMDTAINED 180
           GVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDA+TAAAMDTAINED
Sbjct: 121 GVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDANTAAAMDTAINED 180

Query: 181 DSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSG 240
           DSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSG
Sbjct: 181 DSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSG 240

Query: 241 IENMLMHYVPEVKGVEQEFDVEEEDVKSTSQME 273
           IENMLMHYVPEVKGVEQEFDVEEEDVKSTSQME
Sbjct: 241 IENMLMHYVPEVKGVEQEFDVEEEDVKSTSQME 273

BLAST of Csor.00g124560 vs. ExPASy TrEMBL
Match: A0A6J1H8H2 (nifU-like protein 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111461505 PE=3 SV=1)

HSP 1 Score: 484 bits (1246), Expect = 9.87e-172
Identity = 246/273 (90.11%), Postives = 260/273 (95.24%), Query Frame = 0

Query: 1   MRGLGSLIVRARRISPLGSHGVGKIRFFSAATTSSIFGGKEAASCSVSSRTCSDPLLRSW 60
           MRGLGSLIVRARRI  L S GVGKIRF SAA TSS+F G+EAAS SVSSRT SD  LRSW
Sbjct: 1   MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSW 60

Query: 61  RWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD 120
           +W++FGGQ+RTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLA+ALYGVD
Sbjct: 61  KWNRFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGVD 120

Query: 121 GVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDASTAAAMDTAINED 180
           GV+RVFFGSDFVTVTKSN+ASWDFLKPEIFA+IMDFYSSGQPLFLD+ TAAAMDTAINED
Sbjct: 121 GVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINED 180

Query: 181 DSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSG 240
           DSETVAMIKELLETRIRP VQDDGGDIEYRGY+EETGIVKLRMQGACSGCPSSSVTLKSG
Sbjct: 181 DSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKSG 240

Query: 241 IENMLMHYVPEVKGVEQEFDVEEEDVKSTSQME 273
           IENMLMHYVPEVKGVEQEFDVEE+D+KSTSQME
Sbjct: 241 IENMLMHYVPEVKGVEQEFDVEEDDIKSTSQME 273

BLAST of Csor.00g124560 vs. ExPASy TrEMBL
Match: A0A0A0KE63 (Nfu_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G045100 PE=3 SV=1)

HSP 1 Score: 481 bits (1237), Expect = 2.32e-170
Identity = 244/273 (89.38%), Postives = 255/273 (93.41%), Query Frame = 0

Query: 1   MRGLGSLIVRARRISPLGSHGVGKIRFFSAATTSSIFGGKEAASCSVSSRTCSDPLLRSW 60
           M+GLG LI+RARRI  LG HG G  RFFSAATTSS+FGG+E ASCS+SS  CSD  LRSW
Sbjct: 1   MKGLGRLILRARRIPSLGFHGGGNTRFFSAATTSSMFGGEEPASCSISSTICSDSSLRSW 60

Query: 61  RWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD 120
           +  QFGGQ+RTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD
Sbjct: 61  KSIQFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD 120

Query: 121 GVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDASTAAAMDTAINED 180
           GV+RVFFGSDFVTVTKSN+ASWDFLKPEIFA+IMDFYSSGQPLFLD+ TAAAMDTAI ED
Sbjct: 121 GVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAIKED 180

Query: 181 DSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSG 240
           DSETVAMIKELLETRIRP VQDDGGDIEYRGYNEETGIV LRMQGACSGCPSSSVTLKSG
Sbjct: 181 DSETVAMIKELLETRIRPAVQDDGGDIEYRGYNEETGIVTLRMQGACSGCPSSSVTLKSG 240

Query: 241 IENMLMHYVPEVKGVEQEFDVEEEDVKSTSQME 273
           IENMLMHYVPEVKGVEQEFDVEEEDVKSTSQME
Sbjct: 241 IENMLMHYVPEVKGVEQEFDVEEEDVKSTSQME 273

BLAST of Csor.00g124560 vs. ExPASy TrEMBL
Match: A0A6J1JTA1 (nifU-like protein 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111487295 PE=3 SV=1)

HSP 1 Score: 479 bits (1234), Expect = 6.65e-170
Identity = 243/273 (89.01%), Postives = 257/273 (94.14%), Query Frame = 0

Query: 1   MRGLGSLIVRARRISPLGSHGVGKIRFFSAATTSSIFGGKEAASCSVSSRTCSDPLLRSW 60
           MRGLGSLIVRARRI  L   GVGKIRF SAA TSS+F G+EAAS SVSSRTCSD  LRSW
Sbjct: 1   MRGLGSLIVRARRIPGLNPRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTCSDSYLRSW 60

Query: 61  RWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD 120
           +W++FGGQ+RTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLA+ALYGVD
Sbjct: 61  KWNRFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGVD 120

Query: 121 GVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDASTAAAMDTAINED 180
           GV+RVFFGSDFVTV KSN+ASWDFLKPEIFA+IMDFYSSGQPLFLD+ TAAAMDTAINED
Sbjct: 121 GVVRVFFGSDFVTVRKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINED 180

Query: 181 DSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSG 240
           DSETVAMIKELLETRIRP VQDDGGDIEYRGY+EETGIV LRMQGACSGCPSSSVTLKSG
Sbjct: 181 DSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVNLRMQGACSGCPSSSVTLKSG 240

Query: 241 IENMLMHYVPEVKGVEQEFDVEEEDVKSTSQME 273
           IENMLMHYVPEVKGVEQ FD+EE+DVKSTSQME
Sbjct: 241 IENMLMHYVPEVKGVEQVFDIEEDDVKSTSQME 273

BLAST of Csor.00g124560 vs. TAIR 10
Match: AT3G20970.1 (NFU domain protein 4 )

HSP 1 Score: 355.1 bits (910), Expect = 4.9e-98
Identity = 186/282 (65.96%), Postives = 223/282 (79.08%), Query Frame = 0

Query: 1   MRGLGSLIVRARRISPLGSHGVGKIRFFSAATTSSIFGGKE----AASCSVSSRTCSDPL 60
           M+G+  L+    RI   G   V      +++++SS+   +     +A+  ++SRT    L
Sbjct: 1   MKGIARLVTSLSRIG--GRKVVSGTSTVTSSSSSSLLLSRRSLFISATNLLNSRTKDSAL 60

Query: 61  ------LRSWRWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNS 120
                 L + +W+  GGQ+RTMFI+TQSTPNPSSLMFYPGKPVMEVGSADFPN RSA+ S
Sbjct: 61  PSLNSSLLAQKWNFLGGQRRTMFIQTQSTPNPSSLMFYPGKPVMEVGSADFPNVRSALGS 120

Query: 121 PLAKALYGVDGVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDASTA 180
           PLAK++Y +DGV+RVFFGSDFVTVTKS++ SWD LKPEIFA++MDFYSSGQPLFLD+  A
Sbjct: 121 PLAKSIYSIDGVVRVFFGSDFVTVTKSDDVSWDILKPEIFAAVMDFYSSGQPLFLDSQAA 180

Query: 181 AAMDTAINEDDSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGC 240
           AA DTAI+EDDSETVAMIKELLETRIRP VQDDGGDIEY G++ E+GIVKLRMQGACSGC
Sbjct: 181 AAKDTAISEDDSETVAMIKELLETRIRPAVQDDGGDIEYCGFDPESGIVKLRMQGACSGC 240

Query: 241 PSSSVTLKSGIENMLMHYVPEVKGVEQEFDVEEEDVKSTSQM 273
           PSSSVTLKSGIENMLMHYV EVKGVEQEFD E+E+   + +M
Sbjct: 241 PSSSVTLKSGIENMLMHYVSEVKGVEQEFDGEDEEGTLSGEM 280

BLAST of Csor.00g124560 vs. TAIR 10
Match: AT1G51390.1 (NFU domain protein 5 )

HSP 1 Score: 342.8 bits (878), Expect = 2.5e-94
Identity = 170/221 (76.92%), Postives = 193/221 (87.33%), Query Frame = 0

Query: 48  SSRTCSDPLLRSWRWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSA 107
           +SR CS      + W +   Q+RTMFI+TQSTPNPSSLMF PGKPVME+GSADFPN+RSA
Sbjct: 54  ASRNCSRSSSFPFNWIE---QRRTMFIQTQSTPNPSSLMFSPGKPVMEIGSADFPNSRSA 113

Query: 108 MNSPLAKALYGVDGVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDA 167
           M+SPLAKA++ +DGV+RVF+GSDFVTVTKS++ +WD LKP+IFA +MDFYSSGQPLFLD+
Sbjct: 114 MSSPLAKAIFAIDGVVRVFYGSDFVTVTKSDDVTWDILKPDIFAVVMDFYSSGQPLFLDS 173

Query: 168 STAAAMDTAINEDDSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGAC 227
              AA DTAI+EDDSETVAMIKELLETRIRP+VQDDGGDIEY G++ ETGIVKLRMQGAC
Sbjct: 174 QATAAKDTAIHEDDSETVAMIKELLETRIRPSVQDDGGDIEYCGFDTETGIVKLRMQGAC 233

Query: 228 SGCPSSSVTLKSGIENMLMHYVPEVKGVEQEFDVEEEDVKS 269
           SGCPSSSVTLKSGIENMLMHYV EVKGVEQEFD EEE   S
Sbjct: 234 SGCPSSSVTLKSGIENMLMHYVSEVKGVEQEFDGEEEGTSS 271

BLAST of Csor.00g124560 vs. TAIR 10
Match: AT3G20970.2 (NFU domain protein 4 )

HSP 1 Score: 265.0 bits (676), Expect = 6.7e-71
Identity = 139/223 (62.33%), Postives = 171/223 (76.68%), Query Frame = 0

Query: 1   MRGLGSLIVRARRISPLGSHGVGKIRFFSAATTSSIFGGKE----AASCSVSSRTCSDPL 60
           M+G+  L+    RI   G   V      +++++SS+   +     +A+  ++SRT    L
Sbjct: 1   MKGIARLVTSLSRIG--GRKVVSGTSTVTSSSSSSLLLSRRSLFISATNLLNSRTKDSAL 60

Query: 61  ------LRSWRWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNS 120
                 L + +W+  GGQ+RTMFI+TQSTPNPSSLMFYPGKPVMEVGSADFPN RSA+ S
Sbjct: 61  PSLNSSLLAQKWNFLGGQRRTMFIQTQSTPNPSSLMFYPGKPVMEVGSADFPNVRSALGS 120

Query: 121 PLAKALYGVDGVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDASTA 180
           PLAK++Y +DGV+RVFFGSDFVTVTKS++ SWD LKPEIFA++MDFYSSGQPLFLD+  A
Sbjct: 121 PLAKSIYSIDGVVRVFFGSDFVTVTKSDDVSWDILKPEIFAAVMDFYSSGQPLFLDSQAA 180

Query: 181 AAMDTAINEDDSETVAMIKELLETRIRPTVQDDGGDIEYRGYN 214
           AA DTAI+EDDSETVAMIKELLETRIRP VQDDGGDIEY G++
Sbjct: 181 AAKDTAISEDDSETVAMIKELLETRIRPAVQDDGGDIEYCGFD 221

BLAST of Csor.00g124560 vs. TAIR 10
Match: AT5G49940.1 (NIFU-like protein 2 )

HSP 1 Score: 60.1 bits (144), Expect = 3.3e-09
Identity = 38/109 (34.86%), Postives = 60/109 (55.05%), Query Frame = 0

Query: 157 YSSGQPLFLDASTAAAMDTAINEDDSETVAMIKELLET---RIRPTVQDDGGDIEYRGYN 216
           + S + LF  +    A+ T    D    V + +E +E+    IRP +  DGG++    + 
Sbjct: 58  FLSSRQLFRRSKVVKAVAT---PDPILEVPLTEENVESVLDEIRPYLMSDGGNVAL--HE 117

Query: 217 EETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVEQEFDVE 263
            +  IV++++QGAC  CPSS++T+K GIE  LM  +PE+  VE   D E
Sbjct: 118 IDGNIVRVKLQGACGSCPSSTMTMKMGIERRLMEKIPEIVAVEALPDEE 161

BLAST of Csor.00g124560 vs. TAIR 10
Match: AT5G49940.2 (NIFU-like protein 2 )

HSP 1 Score: 60.1 bits (144), Expect = 3.3e-09
Identity = 38/109 (34.86%), Postives = 60/109 (55.05%), Query Frame = 0

Query: 157 YSSGQPLFLDASTAAAMDTAINEDDSETVAMIKELLET---RIRPTVQDDGGDIEYRGYN 216
           + S + LF  +    A+ T    D    V + +E +E+    IRP +  DGG++    + 
Sbjct: 58  FLSSRQLFRRSKVVKAVAT---PDPILEVPLTEENVESVLDEIRPYLMSDGGNVAL--HE 117

Query: 217 EETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVEQEFDVE 263
            +  IV++++QGAC  CPSS++T+K GIE  LM  +PE+  VE   D E
Sbjct: 118 IDGNIVRVKLQGACGSCPSSTMTMKMGIERRLMEKIPEIVAVEALPDEE 161

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LIG66.9e-9765.96NifU-like protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFU4 PE=2... [more]
Q9C8J23.5e-9376.92NifU-like protein 5, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFU5 PE=2... [more]
Q8SY961.0e-6059.70NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila melanogas... [more]
Q9QZ231.8e-6057.92NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Mus musculus OX=1009... [more]
B4PZ523.0e-6059.70NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila yakuba OX... [more]
Match NameE-valueIdentityDescription
KAG6578816.17.68e-192100.00NifU-like protein 4, mitochondrial, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022939962.11.05e-18998.90nifU-like protein 4, mitochondrial [Cucurbita moschata][more]
XP_022992592.14.08e-18797.80nifU-like protein 4, mitochondrial [Cucurbita maxima][more]
XP_023550903.15.80e-18797.80nifU-like protein 4, mitochondrial [Cucurbita pepo subsp. pepo][more]
KAG7016340.11.31e-17799.21NifU-like protein 4, mitochondrial, partial [Cucurbita argyrosperma subsp. argyr... [more]
Match NameE-valueIdentityDescription
A0A6J1FIB05.06e-19098.90nifU-like protein 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111445668... [more]
A0A6J1JQB11.98e-18797.80nifU-like protein 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111488894 P... [more]
A0A6J1H8H29.87e-17290.11nifU-like protein 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111461505... [more]
A0A0A0KE632.32e-17089.38Nfu_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G045100 PE=3 ... [more]
A0A6J1JTA16.65e-17089.01nifU-like protein 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111487295 P... [more]
Match NameE-valueIdentityDescription
AT3G20970.14.9e-9865.96NFU domain protein 4 [more]
AT1G51390.12.5e-9476.92NFU domain protein 5 [more]
AT3G20970.26.7e-7162.33NFU domain protein 4 [more]
AT5G49940.13.3e-0934.86NIFU-like protein 2 [more]
AT5G49940.23.3e-0934.86NIFU-like protein 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014824Scaffold protein Nfu/NifU, N-terminalSMARTSM00932Nfu_N_3acoord: 74..160
e-value: 5.4E-42
score: 155.5
IPR014824Scaffold protein Nfu/NifU, N-terminalPFAMPF08712Nfu_Ncoord: 74..160
e-value: 2.2E-28
score: 98.2
IPR001075NIF system FeS cluster assembly, NifU, C-terminalPFAMPF01106NifUcoord: 188..256
e-value: 7.3E-29
score: 99.7
IPR036498Scaffold protein Nfu/NifU, N-terminal domain superfamilyGENE3D3.30.1370.70coord: 64..166
e-value: 5.7E-35
score: 121.2
IPR036498Scaffold protein Nfu/NifU, N-terminal domain superfamilySUPERFAMILY110836Hypothetical protein SAV1430coord: 72..162
IPR034904Fe-S cluster assembly domain superfamilyGENE3D3.30.300.130coord: 182..257
e-value: 4.0E-34
score: 118.5
IPR034904Fe-S cluster assembly domain superfamilySUPERFAMILY117916Fe-S cluster assembly (FSCA) domain-likecoord: 174..260
NoneNo IPR availablePANTHERPTHR11178IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATEDcoord: 27..268
NoneNo IPR availablePANTHERPTHR11178:SF43NIFU-LIKE PROTEIN 5, MITOCHONDRIALcoord: 27..268

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g124560.m01Csor.00g124560.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016226 iron-sulfur cluster assembly
biological_process GO:0097428 protein maturation by iron-sulfur cluster transfer
cellular_component GO:0005739 mitochondrion
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0051536 iron-sulfur cluster binding