Csor.00g123370 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g123370
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description2-hydroxyacyl-CoA lyase-like
LocationCsor_Chr14: 11219476 .. 11221567 (+)
RNA-Seq ExpressionCsor.00g123370
SyntenyCsor.00g123370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintronterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCATTTCACTCTCTTCCGCACTTCGTTTCTCCCCCACCCCGAAATCTCAAGCTCCAATGGGGGATTCCGCCGGAGATCTTCCCAGTGACCTCAAATTTCACGGTCCGATTAACGGAAACATCCTTGCCGCCATGGCGCTGGCCCGCTGCGGCGTCGAACGAATGTTCGGCGTCGTTGGAATCCCTGTTACCTCTCTGGCCACTCGCGCCGTTTCTCTTGGCATCCGATTCATCGCCTTTCACAATGAACAATCGGCTGGCTATGCCTTCTGCTTATGGCTATCTAACGGGCCGCCCTGGTGTGCTTCTTACTGTTTCTGGCCCTGGTTGTGTTCATGGCCTTGCAGGGATTTCTAACGCCATGGTTAATGCTTGGCCTTTTGTCATGATCTCTGGTTCTTGTCATAGTTCATATTTCGGCCGTGGTGATTTTCAGGAACTTGATCAGGTTGAGGCTGTGAAGCCCTTTTCCAAATTTTCCGTGAAGGCCACGGATATTTCCGAGATTCCCAATTGCGTTGCTCGAGTTCTTAATATTGCCGTTTCTGGTCGGCCTGGTGGGTGCTATATTGATCTCCCGTCCGATATTTTGCACCAGACTATTTCCGAGTCTGAGGCGGAGAGATTATTGGGAGCAGCGGAGAAATTTACACACCGCGAGGTGATTTATAGAGTTCCGAATTCGCAGATTGAGGCAGCGATTTCGTTGTTGAGACGTGCGGAACGGCCATTGATTGTGTTTGGTAAAGGGGCGGCGATTGCTCGTGCTGAGGGGCCATTGAAGAAGCTTGTGGAGAGCACTGGAATTCCATTTCTTCCTACTCCAATGGGTAAAGGATTGCTTCCTGATTCACACGAGTTAGCGGCTACCGCCGCTAGGTCGTTGGCGATTGGTAAGTGCGATGTTGCGCTTGTGGTTGGGGCGAGGCTTAATTGGTTGTTGCACTTTGGCGAGCCACCTAAATGGTCCAAGGATGTGAAGTTCATTTTGGTTGATATTTGCAAGGAAGAAATTGAATTAAGAAAGCCCCATTTGGGTTTGATTGGTGATGCAAAAGAGGTATTGGAATCAATAAACAAGGAGATTAAGGATGACCCATTTTGTCTTGGGAAGTCTCATCCATGGGTTGAAGCCATTTCGCAGAAATCAAAGGACAATGTGGCGAAAATGGAAGTTCAGCTGGCGAGGGATGTTGTGCCATTCAATTTCTTGACACCAATGAGAATCATTCGAGATGCTATTGCGGCTCTCGGTAGCCCTGCGCCCATATTGGTTTCTGAAGGGGCCAATACAATGGATGTTGGTCGCTCAGTTTTGATTCAGACTGAGCCAAGGACCAGGTTGGATGCAGGAACATGGGGCACAATGGGGGTTGGCTTGGGCTATTGCATTGCAGCTGCTGTTGCTTCGCCTGATCGGTTGGTAGTCGCGGTCGAAGGCGATTCTGGATTTGGGTTCAGTGCCATGGAAGTTGAGGTATTCACTCTCCCCTGCACTTTATTTTCATCCTGTTGGTTTCTTGCTAATGATCAGAATGTTATATGCTTGGTATCTGATTCTTAGCTCATTCAAGAGTGTCTTTAGCTAGCCAGTATTCTTTATTGAGTTTGTGCATTGTTGTGCAATCCTAACATAAATGTTCATATGCCCGTCCTCTCAATCTAACTTCATGTTCAGACAAGTTTTTAGCTTTTGTACTTAAAAGTGTAGATGCTTTTCCTATTTTGTGGGAGATACATTCTCATCTGGTCCACATTTGTCTGGTTTTGAACAGACGCTGGTGCGGTACCAGTTGGCAGTGGTTGTGATAGTTTTCAACAACGGTGGTGTATATGGTGGCGACCGGAGAACACCACAAGAAATAACCGGACCCTATAAGCATGATCCTGCGCCAACCTCATTTGTCCCATCAGCAAGTTACCACAAAATGATGGAGGCTTTTGGAGGTAGAGGATACCATGTTGAAACGGTGGAGGAACTAAAATCTGCACTAGCCGAGTCGTTCTCGGCTCGAAAACCAGCAGTCATAAACGTCACGATCGATCCCTATGCCGGTGCAGAAAGTGGGAGGCTGCAACACAAGAATTGA

mRNA sequence

ATGTCATTTCACTCTCTTCCGCACTTCGTTTCTCCCCCACCCCGAAATCTCAAGCTCCAATGGGGGATTCCGCCGGAGATCTTCCCAGTGACCTCAAATTTCACGGTCCGATTAACGGAAACATCCTTGCCGCCATGGCGCTGGCCCGCTGCGGCGTCGAACGAATGTTCGGCGTCGTTGGAATCCCTGTTACCTCTCTGGCCACTCGCGCCGTTTCTCTTGGCATCCGATTCATCGCCTTTCACAATGAACAATCGGCTGGCTATGCCTTCTGCTTATGGCTATCTAACGGGCCGCCCTGGTGTGCTTCTTACTGTTTCTGGCCCTGGTTGTGTTCATGGCCTTGCAGGGATTTCTAACGCCATGGTTAATGCTTGGCCTTTTGTCATGATCTCTGGTTCTTGTCATAGTTCATATTTCGGCCGTGGTGATTTTCAGGAACTTGATCAGGTTGAGGCTGTGAAGCCCTTTTCCAAATTTTCCGTGAAGGCCACGGATATTTCCGAGATTCCCAATTGCGTTGCTCGAGTTCTTAATATTGCCGTTTCTGGTCGGCCTGGTGGGTGCTATATTGATCTCCCGTCCGATATTTTGCACCAGACTATTTCCGAGTCTGAGGCGGAGAGATTATTGGGAGCAGCGGAGAAATTTACACACCGCGAGGTGATTTATAGAGTTCCGAATTCGCAGATTGAGGCAGCGATTTCGTTGTTGAGACGTGCGGAACGGCCATTGATTGTGTTTGGTAAAGGGGCGGCGATTGCTCGTGCTGAGGGGCCATTGAAGAAGCTTGTGGAGAGCACTGGAATTCCATTTCTTCCTACTCCAATGGGTAAAGGATTGCTTCCTGATTCACACGAGTTAGCGGCTACCGCCGCTAGGTCGTTGGCGATTGGTAAGTGCGATGTTGCGCTTGTGGTTGGGGCGAGGCTTAATTGGTTGTTGCACTTTGGCGAGCCACCTAAATGGTCCAAGGATGTGAAGTTCATTTTGGTTGATATTTGCAAGGAAGAAATTGAATTAAGAAAGCCCCATTTGGGTTTGATTGGTGATGCAAAAGAGGTATTGGAATCAATAAACAAGGAGATTAAGGATGACCCATTTTGTCTTGGGAAGTCTCATCCATGGGTTGAAGCCATTTCGCAGAAATCAAAGGACAATGTGGCGAAAATGGAAGTTCAGCTGGCGAGGGATGTTGTGCCATTCAATTTCTTGACACCAATGAGAATCATTCGAGATGCTATTGCGGCTCTCGGTAGCCCTGCGCCCATATTGGTTTCTGAAGGGGCCAATACAATGGATGTTGGTCGCTCAGTTTTGATTCAGACTGAGCCAAGGACCAGGTTGGATGCAGGAACATGGGGCACAATGGGGGTTGGCTTGGGCTATTGCATTGCAGCTGCTGTTGCTTCGCCTGATCGGTTGGTAGTCGCGGTCGAAGGCGATTCTGGATTTGGGTTCAGTGCCATGGAAGTTGAGACGCTGGTGCGGTACCAGTTGGCAGTGGTTGTGATAGTTTTCAACAACGGTGGTGTATATGGTGGCGACCGGAGAACACCACAAGAAATAACCGGACCCTATAAGCATGATCCTGCGCCAACCTCATTTGTCCCATCAGCAAGTTACCACAAAATGATGGAGGCTTTTGGAGGTAGAGGATACCATGTTGAAACGGTGGAGGAACTAAAATCTGCACTAGCCGAGTCGTTCTCGGCTCGAAAACCAGCAGTCATAAACGTCACGATCGATCCCTATGCCGGTGCAGAAAGTGGGAGGCTGCAACACAAGAATTGA

Coding sequence (CDS)

ATGTCATTTCACTCTCTTCCGCACTTCGTTTCTCCCCCACCCCGAAATCTCAAGCTCCAATGGGGGATTCCGCCGGAGATCTTCCCAGTGACCTCAAATTTCACGGTCCGATTAACGGAAACATCCTTGCCGCCATGGCGCTGGCCCGCTGCGGCGTCGAACGAATGTTCGGCGTCGTTGGAATCCCTGTTACCTCTCTGGCCACTCGCGCCGTTTCTCTTGGCATCCGATTCATCGCCTTTCACAATGAACAATCGGCTGGCTATGCCTTCTGCTTATGGCTATCTAACGGGCCGCCCTGGTGTGCTTCTTACTGTTTCTGGCCCTGGTTGTGTTCATGGCCTTGCAGGGATTTCTAACGCCATGGTTAATGCTTGGCCTTTTGTCATGATCTCTGGTTCTTGTCATAGTTCATATTTCGGCCGTGGTGATTTTCAGGAACTTGATCAGGTTGAGGCTGTGAAGCCCTTTTCCAAATTTTCCGTGAAGGCCACGGATATTTCCGAGATTCCCAATTGCGTTGCTCGAGTTCTTAATATTGCCGTTTCTGGTCGGCCTGGTGGGTGCTATATTGATCTCCCGTCCGATATTTTGCACCAGACTATTTCCGAGTCTGAGGCGGAGAGATTATTGGGAGCAGCGGAGAAATTTACACACCGCGAGGTGATTTATAGAGTTCCGAATTCGCAGATTGAGGCAGCGATTTCGTTGTTGAGACGTGCGGAACGGCCATTGATTGTGTTTGGTAAAGGGGCGGCGATTGCTCGTGCTGAGGGGCCATTGAAGAAGCTTGTGGAGAGCACTGGAATTCCATTTCTTCCTACTCCAATGGGTAAAGGATTGCTTCCTGATTCACACGAGTTAGCGGCTACCGCCGCTAGGTCGTTGGCGATTGGTAAGTGCGATGTTGCGCTTGTGGTTGGGGCGAGGCTTAATTGGTTGTTGCACTTTGGCGAGCCACCTAAATGGTCCAAGGATGTGAAGTTCATTTTGGTTGATATTTGCAAGGAAGAAATTGAATTAAGAAAGCCCCATTTGGGTTTGATTGGTGATGCAAAAGAGGTATTGGAATCAATAAACAAGGAGATTAAGGATGACCCATTTTGTCTTGGGAAGTCTCATCCATGGGTTGAAGCCATTTCGCAGAAATCAAAGGACAATGTGGCGAAAATGGAAGTTCAGCTGGCGAGGGATGTTGTGCCATTCAATTTCTTGACACCAATGAGAATCATTCGAGATGCTATTGCGGCTCTCGGTAGCCCTGCGCCCATATTGGTTTCTGAAGGGGCCAATACAATGGATGTTGGTCGCTCAGTTTTGATTCAGACTGAGCCAAGGACCAGGTTGGATGCAGGAACATGGGGCACAATGGGGGTTGGCTTGGGCTATTGCATTGCAGCTGCTGTTGCTTCGCCTGATCGGTTGGTAGTCGCGGTCGAAGGCGATTCTGGATTTGGGTTCAGTGCCATGGAAGTTGAGACGCTGGTGCGGTACCAGTTGGCAGTGGTTGTGATAGTTTTCAACAACGGTGGTGTATATGGTGGCGACCGGAGAACACCACAAGAAATAACCGGACCCTATAAGCATGATCCTGCGCCAACCTCATTTGTCCCATCAGCAAGTTACCACAAAATGATGGAGGCTTTTGGAGGTAGAGGATACCATGTTGAAACGGTGGAGGAACTAAAATCTGCACTAGCCGAGTCGTTCTCGGCTCGAAAACCAGCAGTCATAAACGTCACGATCGATCCCTATGCCGGTGCAGAAAGTGGGAGGCTGCAACACAAGAATTGA

Protein sequence

MSFHSLPHFVSPPPRNLKLQWGIPPEIFPVTSNFTVRLTETSLPPWRWPAAASNECSASLESLLPLWPLAPFLLASDSSPFTMNNRLAMPSAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPFVMISGSCHSSYFGRGDFQELDQVEAVKPFSKFSVKATDISEIPNCVARVLNIAVSGRPGGCYIDLPSDILHQTISESEAERLLGAAEKFTHREVIYRVPNSQIEAAISLLRRAERPLIVFGKGAAIARAEGPLKKLVESTGIPFLPTPMGKGLLPDSHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFILVDICKEEIELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVEAISQKSKDNVAKMEVQLARDVVPFNFLTPMRIIRDAIAALGSPAPILVSEGANTMDVGRSVLIQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLAVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSASYHKMMEAFGGRGYHVETVEELKSALAESFSARKPAVINVTIDPYAGAESGRLQHKN
Homology
BLAST of Csor.00g123370 vs. ExPASy Swiss-Prot
Match: Q9LF46 (2-hydroxyacyl-CoA lyase OS=Arabidopsis thaliana OX=3702 GN=HACL PE=1 SV=1)

HSP 1 Score: 824.7 bits (2129), Expect = 6.7e-238
Identity = 399/507 (78.70%), Postives = 453/507 (89.35%), Query Frame = 0

Query: 91  SAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPFVMISGSCHSSYFGRGDFQELDQ 150
           SAYGYLTG+PG+LLTVSGPGCVHGLAG+SNA VN WP VMISGSC     GRGDFQELDQ
Sbjct: 66  SAYGYLTGKPGILLTVSGPGCVHGLAGLSNAWVNTWPMVMISGSCDQRDVGRGDFQELDQ 125

Query: 151 VEAVKPFSKFSVKATDISEIPNCVARVLNIAVSGRPGGCYIDLPSDILHQTISESEAERL 210
           +EAVK FSK S KA D+ EIP+CV+RVL+ AVSGRPGGCY+D+P+D+L Q ISESEA++L
Sbjct: 126 IEAVKAFSKLSEKAKDVREIPDCVSRVLDRAVSGRPGGCYLDIPTDVLRQKISESEADKL 185

Query: 211 LGAAEKFTHREVIYRVPNSQIEAAISLLRRAERPLIVFGKGAAIARAEGPLKKLVESTGI 270
           +   E+    E I     S+IE+A+SLLR+AERPLIVFGKGAA +RAE  LKKLVE TGI
Sbjct: 186 VDEVERSRKEEPIRGSLRSEIESAVSLLRKAERPLIVFGKGAAYSRAEDELKKLVEITGI 245

Query: 271 PFLPTPMGKGLLPDSHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFI 330
           PFLPTPMGKGLLPD+HE +ATAARSLAIGKCDVALVVGARLNWLLHFGE PKW KDVKFI
Sbjct: 246 PFLPTPMGKGLLPDTHEFSATAARSLAIGKCDVALVVGARLNWLLHFGESPKWDKDVKFI 305

Query: 331 LVDICKEEIELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVEAISQKSKDNVAK 390
           LVD+ +EEIELRKPHLG++GDAK V+  +N+EIKDDPFCLGKS+ WVE+IS+K+K+N  K
Sbjct: 306 LVDVSEEEIELRKPHLGIVGDAKTVIGLLNREIKDDPFCLGKSNSWVESISKKAKENGEK 365

Query: 391 MEVQLARDVVPFNFLTPMRIIRDAIAALGSPAPILVSEGANTMDVGRSVLIQTEPRTRLD 450
           ME+QLA+DVVPFNFLTPMRIIRDAI A+  P+P++VSEGANTMDVGRSVL+Q EPRTRLD
Sbjct: 366 MEIQLAKDVVPFNFLTPMRIIRDAILAVEGPSPVVVSEGANTMDVGRSVLVQKEPRTRLD 425

Query: 451 AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLAVVVIVFNNG 510
           AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRY LAVV+IVFNNG
Sbjct: 426 AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYNLAVVIIVFNNG 485

Query: 511 GVYGGDRRTPQEITGPYKHDPAPTSFVPSASYHKMMEAFGGRGYHVETVEELKSALAESF 570
           GVYGGDRR P+EI+GP+K DPAPTSFVP+A YHK++EAFGG+GY VET +ELKSALAESF
Sbjct: 486 GVYGGDRRGPEEISGPHKEDPAPTSFVPNAGYHKLIEAFGGKGYIVETPDELKSALAESF 545

Query: 571 SARKPAVINVTIDPYAGAESGRLQHKN 598
           +ARKPAV+NV IDP+AGAESGRLQHKN
Sbjct: 546 AARKPAVVNVIIDPFAGAESGRLQHKN 572

BLAST of Csor.00g123370 vs. ExPASy Swiss-Prot
Match: Q0JMH0 (2-hydroxyacyl-CoA lyase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0505400 PE=3 SV=3)

HSP 1 Score: 798.9 bits (2062), Expect = 3.9e-230
Identity = 386/511 (75.54%), Postives = 443/511 (86.69%), Query Frame = 0

Query: 91  SAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPFVMISGSCHSSYFGRGDFQELDQ 150
           +AYG+LTG PG+LLTVSGPGCVHGLAG+S+A  NAWP +M+SGSC     GRGDFQELDQ
Sbjct: 67  AAYGFLTGSPGLLLTVSGPGCVHGLAGLSHATANAWPLLMVSGSCSQPDAGRGDFQELDQ 126

Query: 151 VEAVKPFSKFSVKATDISEIPNCVARVLNIAVSGRPGGCYIDLPSDILHQTISESEAERL 210
           + A KPF K +VKAT I++IP  V + L   VSGRPGGCY+D+PSD+LHQT++ESEA  L
Sbjct: 127 IAATKPFIKIAVKATTIADIPRLVFQALAATVSGRPGGCYLDIPSDVLHQTLTESEAAAL 186

Query: 211 LGAA----EKFTHREVIYRVPNSQIEAAISLLRRAERPLIVFGKGAAIARAEGPLKKLVE 270
           + AA     K       ++  +  IE A  LLRRAERPL+VFGKGAA +RAE  + KLV+
Sbjct: 187 IDAAAADSAKSDSSPPKHKSLDEGIEKAAELLRRAERPLVVFGKGAAYSRAEDAIWKLVD 246

Query: 271 STGIPFLPTPMGKGLLPDSHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKD 330
           +TGIPFLPTPMGKG++PD+H L+ATAARSLAIG+CDVALVVGARLNWLLHFGEPPKWSKD
Sbjct: 247 TTGIPFLPTPMGKGVVPDTHPLSATAARSLAIGQCDVALVVGARLNWLLHFGEPPKWSKD 306

Query: 331 VKFILVDICKEEIELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVEAISQKSKD 390
           VKFILVD+C+EEIELRKPH+G++GDAK V+E IN+EIKD PFCL  SHPWVEAI++K++D
Sbjct: 307 VKFILVDVCEEEIELRKPHVGIVGDAKRVVELINREIKDQPFCLAPSHPWVEAITKKARD 366

Query: 391 NVAKMEVQLARDVVPFNFLTPMRIIRDAIAALGSPAPILVSEGANTMDVGRSVLIQTEPR 450
           NV KME QLA+DVVPFNFLTP+RIIRDAI A G+PAP++VSEGANTMDVGR+VL+Q EPR
Sbjct: 367 NVLKMEAQLAKDVVPFNFLTPLRIIRDAILAEGNPAPVVVSEGANTMDVGRAVLVQNEPR 426

Query: 451 TRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLAVVVIV 510
           TRLDAGTWGTMGVGLG+C+AAAVA PDRLVVAVEGDSGFGFSAMEVETLVRYQL VVVIV
Sbjct: 427 TRLDAGTWGTMGVGLGFCVAAAVAEPDRLVVAVEGDSGFGFSAMEVETLVRYQLPVVVIV 486

Query: 511 FNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSASYHKMMEAFGGRGYHVETVEELKSAL 570
           FNN GVYGGDRR+P EITGPYK DPAPTSFVP+A YHKMMEAFGG+GY VET +ELKSAL
Sbjct: 487 FNNNGVYGGDRRSPDEITGPYKDDPAPTSFVPAAGYHKMMEAFGGKGYLVETPDELKSAL 546

Query: 571 AESFSARKPAVINVTIDPYAGAESGRLQHKN 598
           +ESF ARKPAVINV IDPYAGAESGR+QHKN
Sbjct: 547 SESFRARKPAVINVIIDPYAGAESGRMQHKN 577

BLAST of Csor.00g123370 vs. ExPASy Swiss-Prot
Match: Q8CHM7 (2-hydroxyacyl-CoA lyase 1 OS=Rattus norvegicus OX=10116 GN=Hacl1 PE=1 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 5.7e-112
Identity = 221/509 (43.42%), Postives = 315/509 (61.89%), Query Frame = 0

Query: 91  SAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPFVMISGSCHSSYFGRGDFQELDQ 150
           SA GYLTGRPGV L VSGPG +H L G++NA +N WP ++I GS   +    G FQE  Q
Sbjct: 71  SAVGYLTGRPGVCLVVSGPGLIHALGGMANANMNCWPLIVIGGSSERNQEAMGAFQEFPQ 130

Query: 151 VEAVKPFSKFSVKATDISEIPNCVARVLNIAVSGRPGGCYIDLPSDILHQTISESEAERL 210
           VEA + +SKFS + + I  IP  + + +  ++ GRPG CYID+P+D++  T+ ES     
Sbjct: 131 VEACRLYSKFSARPSSIQHIPFVIEKAVRSSIYGRPGACYIDVPADLV--TLQES----- 190

Query: 211 LGAAEKFTHREVIYRVP-----NSQIEAAISLLRRAERPLIVFGKGAAIARAEGPLKKLV 270
                   ++E     P      S + AA S+LR A++PL++ GKGAA + AE  ++KLV
Sbjct: 191 ---TTSIKYKECCMPPPLSLAETSAVRAAASVLRSAKQPLLIIGKGAAYSHAEDSIRKLV 250

Query: 271 ESTGIPFLPTPMGKGLLPDSHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSK 330
           E   +PFLPTPMGKG++PD+H     AARS A+   DV ++ GARLNW+LHFG PP++  
Sbjct: 251 ELCNLPFLPTPMGKGVVPDNHPNCVGAARSRALQFADVIVLFGARLNWILHFGLPPRYQA 310

Query: 331 DVKFILVDICKEEIELR-KPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVEAISQKS 390
           DVKFI +DIC EE+    +P + L+GD   V + + ++   +P+       W E + +K 
Sbjct: 311 DVKFIQIDICAEELGNNVRPSVTLLGDVNAVSKQLLEQFVKNPWQYPTDSKWWETLREKR 370

Query: 391 KDNVAKMEVQLARDVVPFNFLTPMRIIRDAIAALGSPAPILVSEGANTMDVGRSVLIQTE 450
           K+N A  +   ++  +P N+ T    +++ +         +VSEGANTMD+GR+VL    
Sbjct: 371 KNNEAVSKELASKKSLPMNYYTVFYHVQEQLPR----NCFIVSEGANTMDIGRTVLQNYL 430

Query: 451 PRTRLDAGTWGTMGVGLGYCIAAAVA----SPDRLVVAVEGDSGFGFSAMEVETLVRYQL 510
           PR RLDAG++GTMGVGLG+ IAAAV     SP + V+ VEGDS FGFS MEVET+ RY L
Sbjct: 431 PRHRLDAGSFGTMGVGLGFAIAAAVVAKERSPGQRVICVEGDSAFGFSGMEVETICRYNL 490

Query: 511 AVVVIVFNNGGVYGG-DRRTPQEI---TGPYKHDPAPTSFVPSASYHKMMEAFGGRGYHV 570
            ++++V NN G+Y G D  T  +I    G     P P   +P++ Y ++M AFGG+GY V
Sbjct: 491 PIIILVVNNNGIYQGFDADTWGKILNFQGTATTIP-PMCLLPNSHYEQVMTAFGGKGYFV 550

Query: 571 ETVEELKSALAESF-SARKPAVINVTIDP 585
           +T EEL+ +L ++     KP +IN+ I+P
Sbjct: 551 QTPEELQDSLRQALKDTSKPCLINIMIEP 564

BLAST of Csor.00g123370 vs. ExPASy Swiss-Prot
Match: P0AFI1 (Oxalyl-CoA decarboxylase OS=Escherichia coli O157:H7 OX=83334 GN=oxc PE=3 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 7.4e-112
Identity = 216/510 (42.35%), Postives = 320/510 (62.75%), Query Frame = 0

Query: 91  SAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPFVMISGSCHSSY--FGRGDFQEL 150
           +A G+LT +PG+ LTVS PG ++GL  ++NA VN +P +MISGS   +     +GD++EL
Sbjct: 62  AASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMISGSSDRAIVDLQQGDYEEL 121

Query: 151 DQVEAVKPFSKFSVKATDISEIPNCVARVLNIAVSGRPGGCYIDLPSDILHQTISESEAE 210
           DQ+ A KP++K + +     ++   +AR + ++VSGRPGG Y+DLP+++L  T+ + EA 
Sbjct: 122 DQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPGGVYLDLPANVLAATMEKDEAL 181

Query: 211 RLLGAAEKFTHREVIYRVPNSQIEAAISLLRRAERPLIVFGKGAAIARAEGPLKKLVEST 270
             +   E       +   P S + +AISLL +AERPLI+ GKGAA ++A+  L++ +ES 
Sbjct: 182 TTIVKVE--NPSPALLPCPKS-VTSAISLLAKAERPLIILGKGAAYSQADEQLREFIESA 241

Query: 271 GIPFLPTPMGKGLLPDSHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVK 330
            IPFLP  M KG+L D+H L+A AARS A+   DV ++VGARLNWLL  G+   W+ D +
Sbjct: 242 QIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVMLVGARLNWLLAHGK-KGWAADTQ 301

Query: 331 FILVDICKEEIELRKP-HLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVEAISQKSKDN 390
           FI +DI  +EI+  +P  + ++GD    ++ +  E+K + F       W + ++   + N
Sbjct: 302 FIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELKQNTFT--TPLVWRDILNIHKQQN 361

Query: 391 VAKMEVQLARDVVPFNFLTPMRIIRDAIAALGSPAPILVSEGANTMDVGRSVLIQTEPRT 450
             KM  +L+ D  P N+   +  +RD +    +    LV+EGANT+D  R+++   +PR 
Sbjct: 362 AQKMHEKLSTDTQPLNYFNALSAVRDVLRE--NQDIYLVNEGANTLDNARNIIDMYKPRR 421

Query: 451 RLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLAVVVIVF 510
           RLD GTWG MG+G+GY I A+V S    VVA+EGDS FGFS ME+ET+ RY L V +++F
Sbjct: 422 RLDCGTWGVMGIGMGYAIGASVTSGSP-VVAIEGDSAFGFSGMEIETICRYNLPVTIVIF 481

Query: 511 NNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSASYHKMMEAFGGRGYHVETVEELKSALA 570
           NNGG+Y GD     +++G     P+PT  +  A Y K+M+AF G GY+V T +EL+ AL 
Sbjct: 482 NNGGIYRGD---GVDLSG--AGAPSPTDLLHHARYDKLMDAFRGVGYNVTTTDELRHALT 541

Query: 571 ESFSARKPAVINVTIDPYAGAESGRLQHKN 598
               +RKP +INV IDP AG ESG +   N
Sbjct: 542 TGIQSRKPTIINVVIDPAAGTESGHITKLN 557

BLAST of Csor.00g123370 vs. ExPASy Swiss-Prot
Match: P0AFI0 (Oxalyl-CoA decarboxylase OS=Escherichia coli (strain K12) OX=83333 GN=oxc PE=1 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 7.4e-112
Identity = 216/510 (42.35%), Postives = 320/510 (62.75%), Query Frame = 0

Query: 91  SAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPFVMISGSCHSSY--FGRGDFQEL 150
           +A G+LT +PG+ LTVS PG ++GL  ++NA VN +P +MISGS   +     +GD++EL
Sbjct: 62  AASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMISGSSDRAIVDLQQGDYEEL 121

Query: 151 DQVEAVKPFSKFSVKATDISEIPNCVARVLNIAVSGRPGGCYIDLPSDILHQTISESEAE 210
           DQ+ A KP++K + +     ++   +AR + ++VSGRPGG Y+DLP+++L  T+ + EA 
Sbjct: 122 DQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPGGVYLDLPANVLAATMEKDEAL 181

Query: 211 RLLGAAEKFTHREVIYRVPNSQIEAAISLLRRAERPLIVFGKGAAIARAEGPLKKLVEST 270
             +   E       +   P S + +AISLL +AERPLI+ GKGAA ++A+  L++ +ES 
Sbjct: 182 TTIVKVE--NPSPALLPCPKS-VTSAISLLAKAERPLIILGKGAAYSQADEQLREFIESA 241

Query: 271 GIPFLPTPMGKGLLPDSHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVK 330
            IPFLP  M KG+L D+H L+A AARS A+   DV ++VGARLNWLL  G+   W+ D +
Sbjct: 242 QIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVMLVGARLNWLLAHGK-KGWAADTQ 301

Query: 331 FILVDICKEEIELRKP-HLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVEAISQKSKDN 390
           FI +DI  +EI+  +P  + ++GD    ++ +  E+K + F       W + ++   + N
Sbjct: 302 FIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELKQNTFT--TPLVWRDILNIHKQQN 361

Query: 391 VAKMEVQLARDVVPFNFLTPMRIIRDAIAALGSPAPILVSEGANTMDVGRSVLIQTEPRT 450
             KM  +L+ D  P N+   +  +RD +    +    LV+EGANT+D  R+++   +PR 
Sbjct: 362 AQKMHEKLSTDTQPLNYFNALSAVRDVLRE--NQDIYLVNEGANTLDNARNIIDMYKPRR 421

Query: 451 RLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLAVVVIVF 510
           RLD GTWG MG+G+GY I A+V S    VVA+EGDS FGFS ME+ET+ RY L V +++F
Sbjct: 422 RLDCGTWGVMGIGMGYAIGASVTSGSP-VVAIEGDSAFGFSGMEIETICRYNLPVTIVIF 481

Query: 511 NNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSASYHKMMEAFGGRGYHVETVEELKSALA 570
           NNGG+Y GD     +++G     P+PT  +  A Y K+M+AF G GY+V T +EL+ AL 
Sbjct: 482 NNGGIYRGD---GVDLSG--AGAPSPTDLLHHARYDKLMDAFRGVGYNVTTTDELRHALT 541

Query: 571 ESFSARKPAVINVTIDPYAGAESGRLQHKN 598
               +RKP +INV IDP AG ESG +   N
Sbjct: 542 TGIQSRKPTIINVVIDPAAGTESGHITKLN 557

BLAST of Csor.00g123370 vs. NCBI nr
Match: KAG6581738.1 (2-hydroxyacyl-CoA lyase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1204 bits (3116), Expect = 0.0
Identity = 597/597 (100.00%), Postives = 597/597 (100.00%), Query Frame = 0

Query: 1   MSFHSLPHFVSPPPRNLKLQWGIPPEIFPVTSNFTVRLTETSLPPWRWPAAASNECSASL 60
           MSFHSLPHFVSPPPRNLKLQWGIPPEIFPVTSNFTVRLTETSLPPWRWPAAASNECSASL
Sbjct: 1   MSFHSLPHFVSPPPRNLKLQWGIPPEIFPVTSNFTVRLTETSLPPWRWPAAASNECSASL 60

Query: 61  ESLLPLWPLAPFLLASDSSPFTMNNRLAMPSAYGYLTGRPGVLLTVSGPGCVHGLAGISN 120
           ESLLPLWPLAPFLLASDSSPFTMNNRLAMPSAYGYLTGRPGVLLTVSGPGCVHGLAGISN
Sbjct: 61  ESLLPLWPLAPFLLASDSSPFTMNNRLAMPSAYGYLTGRPGVLLTVSGPGCVHGLAGISN 120

Query: 121 AMVNAWPFVMISGSCHSSYFGRGDFQELDQVEAVKPFSKFSVKATDISEIPNCVARVLNI 180
           AMVNAWPFVMISGSCHSSYFGRGDFQELDQVEAVKPFSKFSVKATDISEIPNCVARVLNI
Sbjct: 121 AMVNAWPFVMISGSCHSSYFGRGDFQELDQVEAVKPFSKFSVKATDISEIPNCVARVLNI 180

Query: 181 AVSGRPGGCYIDLPSDILHQTISESEAERLLGAAEKFTHREVIYRVPNSQIEAAISLLRR 240
           AVSGRPGGCYIDLPSDILHQTISESEAERLLGAAEKFTHREVIYRVPNSQIEAAISLLRR
Sbjct: 181 AVSGRPGGCYIDLPSDILHQTISESEAERLLGAAEKFTHREVIYRVPNSQIEAAISLLRR 240

Query: 241 AERPLIVFGKGAAIARAEGPLKKLVESTGIPFLPTPMGKGLLPDSHELAATAARSLAIGK 300
           AERPLIVFGKGAAIARAEGPLKKLVESTGIPFLPTPMGKGLLPDSHELAATAARSLAIGK
Sbjct: 241 AERPLIVFGKGAAIARAEGPLKKLVESTGIPFLPTPMGKGLLPDSHELAATAARSLAIGK 300

Query: 301 CDVALVVGARLNWLLHFGEPPKWSKDVKFILVDICKEEIELRKPHLGLIGDAKEVLESIN 360
           CDVALVVGARLNWLLHFGEPPKWSKDVKFILVDICKEEIELRKPHLGLIGDAKEVLESIN
Sbjct: 301 CDVALVVGARLNWLLHFGEPPKWSKDVKFILVDICKEEIELRKPHLGLIGDAKEVLESIN 360

Query: 361 KEIKDDPFCLGKSHPWVEAISQKSKDNVAKMEVQLARDVVPFNFLTPMRIIRDAIAALGS 420
           KEIKDDPFCLGKSHPWVEAISQKSKDNVAKMEVQLARDVVPFNFLTPMRIIRDAIAALGS
Sbjct: 361 KEIKDDPFCLGKSHPWVEAISQKSKDNVAKMEVQLARDVVPFNFLTPMRIIRDAIAALGS 420

Query: 421 PAPILVSEGANTMDVGRSVLIQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVE 480
           PAPILVSEGANTMDVGRSVLIQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVE
Sbjct: 421 PAPILVSEGANTMDVGRSVLIQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVE 480

Query: 481 GDSGFGFSAMEVETLVRYQLAVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSA 540
           GDSGFGFSAMEVETLVRYQLAVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSA
Sbjct: 481 GDSGFGFSAMEVETLVRYQLAVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSA 540

Query: 541 SYHKMMEAFGGRGYHVETVEELKSALAESFSARKPAVINVTIDPYAGAESGRLQHKN 597
           SYHKMMEAFGGRGYHVETVEELKSALAESFSARKPAVINVTIDPYAGAESGRLQHKN
Sbjct: 541 SYHKMMEAFGGRGYHVETVEELKSALAESFSARKPAVINVTIDPYAGAESGRLQHKN 597

BLAST of Csor.00g123370 vs. NCBI nr
Match: XP_022955931.1 (2-hydroxyacyl-CoA lyase-like [Cucurbita moschata])

HSP 1 Score: 1014 bits (2621), Expect = 0.0
Identity = 506/507 (99.80%), Postives = 506/507 (99.80%), Query Frame = 0

Query: 91  SAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPFVMISGSCHSSYFGRGDFQELDQ 150
           SAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPFVMISGSCHSSYFGRGDFQELDQ
Sbjct: 72  SAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPFVMISGSCHSSYFGRGDFQELDQ 131

Query: 151 VEAVKPFSKFSVKATDISEIPNCVARVLNIAVSGRPGGCYIDLPSDILHQTISESEAERL 210
           VEAVKPFSKFSVKATDISEIPNCVARVLNIAVSGRPGGCYIDLPSDILHQTISESEAERL
Sbjct: 132 VEAVKPFSKFSVKATDISEIPNCVARVLNIAVSGRPGGCYIDLPSDILHQTISESEAERL 191

Query: 211 LGAAEKFTHREVIYRVPNSQIEAAISLLRRAERPLIVFGKGAAIARAEGPLKKLVESTGI 270
           LGAAEKFTHREVIYRVPNSQIEAAISLLRRAERPLIVFGKGAAIARAEGPLKKLVESTGI
Sbjct: 192 LGAAEKFTHREVIYRVPNSQIEAAISLLRRAERPLIVFGKGAAIARAEGPLKKLVESTGI 251

Query: 271 PFLPTPMGKGLLPDSHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFI 330
           PFLPTPMGKGLLPDSHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFI
Sbjct: 252 PFLPTPMGKGLLPDSHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFI 311

Query: 331 LVDICKEEIELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVEAISQKSKDNVAK 390
           LVDICKEEIELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVEAISQKSKDNVAK
Sbjct: 312 LVDICKEEIELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVEAISQKSKDNVAK 371

Query: 391 MEVQLARDVVPFNFLTPMRIIRDAIAALGSPAPILVSEGANTMDVGRSVLIQTEPRTRLD 450
           MEVQLARDVVPFNFLTPMRIIRDAIAALGSPAPILVSEGANTMDVGRSVLIQTEPRTRLD
Sbjct: 372 MEVQLARDVVPFNFLTPMRIIRDAIAALGSPAPILVSEGANTMDVGRSVLIQTEPRTRLD 431

Query: 451 AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLAVVVIVFNNG 510
           AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLAVVVIVFNNG
Sbjct: 432 AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLAVVVIVFNNG 491

Query: 511 GVYGGDRRTPQEITGPYKHDPAPTSFVPSASYHKMMEAFGGRGYHVETVEELKSALAESF 570
           GVYGGDRRTPQEITGPYKHDPAPTSFVPSASYHKMMEAFGGRGYHVET EELKSALAESF
Sbjct: 492 GVYGGDRRTPQEITGPYKHDPAPTSFVPSASYHKMMEAFGGRGYHVETAEELKSALAESF 551

Query: 571 SARKPAVINVTIDPYAGAESGRLQHKN 597
           SARKPAVINVTIDPYAGAESGRLQHKN
Sbjct: 552 SARKPAVINVTIDPYAGAESGRLQHKN 578

BLAST of Csor.00g123370 vs. NCBI nr
Match: XP_023526990.1 (2-hydroxyacyl-CoA lyase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 998 bits (2579), Expect = 0.0
Identity = 496/507 (97.83%), Postives = 502/507 (99.01%), Query Frame = 0

Query: 91  SAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPFVMISGSCHSSYFGRGDFQELDQ 150
           SAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPFVMISGSCHS+YFGRGDFQELDQ
Sbjct: 72  SAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPFVMISGSCHSTYFGRGDFQELDQ 131

Query: 151 VEAVKPFSKFSVKATDISEIPNCVARVLNIAVSGRPGGCYIDLPSDILHQTISESEAERL 210
           VEAVKPFSKFSVKATDISEIPNCVARVLNIAVSGRPGGCYIDLPSDILHQTISESEAERL
Sbjct: 132 VEAVKPFSKFSVKATDISEIPNCVARVLNIAVSGRPGGCYIDLPSDILHQTISESEAERL 191

Query: 211 LGAAEKFTHREVIYRVPNSQIEAAISLLRRAERPLIVFGKGAAIARAEGPLKKLVESTGI 270
           L A E+FTHREVI+RVPNSQIE AISLL+RAERPLIVFGKGAAIARAEGPLKKLVESTGI
Sbjct: 192 LAAEEEFTHREVIHRVPNSQIETAISLLKRAERPLIVFGKGAAIARAEGPLKKLVESTGI 251

Query: 271 PFLPTPMGKGLLPDSHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFI 330
           PFLPTPMGKGLLPDSHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFI
Sbjct: 252 PFLPTPMGKGLLPDSHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFI 311

Query: 331 LVDICKEEIELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVEAISQKSKDNVAK 390
           LVDICKEEIELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVEAISQKSKDNVAK
Sbjct: 312 LVDICKEEIELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVEAISQKSKDNVAK 371

Query: 391 MEVQLARDVVPFNFLTPMRIIRDAIAALGSPAPILVSEGANTMDVGRSVLIQTEPRTRLD 450
           MEVQLARDVVPFNF TPMRIIRDAI+ALGSPAPILVSEGANTMDVGRSVLIQTEPRTRLD
Sbjct: 372 MEVQLARDVVPFNFFTPMRIIRDAISALGSPAPILVSEGANTMDVGRSVLIQTEPRTRLD 431

Query: 451 AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLAVVVIVFNNG 510
           AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLAVVVIVFNNG
Sbjct: 432 AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLAVVVIVFNNG 491

Query: 511 GVYGGDRRTPQEITGPYKHDPAPTSFVPSASYHKMMEAFGGRGYHVETVEELKSALAESF 570
           GVYGGDRRTPQEITGPYKHDPAPTSFVPSASYHKMMEAFGG+GYHVET EELKSALAESF
Sbjct: 492 GVYGGDRRTPQEITGPYKHDPAPTSFVPSASYHKMMEAFGGKGYHVETPEELKSALAESF 551

Query: 571 SARKPAVINVTIDPYAGAESGRLQHKN 597
           SARKPAVINVTIDPYAGAESGRLQHKN
Sbjct: 552 SARKPAVINVTIDPYAGAESGRLQHKN 578

BLAST of Csor.00g123370 vs. NCBI nr
Match: XP_038893948.1 (2-hydroxyacyl-CoA lyase [Benincasa hispida])

HSP 1 Score: 965 bits (2495), Expect = 0.0
Identity = 476/507 (93.89%), Postives = 492/507 (97.04%), Query Frame = 0

Query: 91  SAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPFVMISGSCHSSYFGRGDFQELDQ 150
           SAYGYLTG PGVLLTVSGPGCVHGLAG+SNAMVNAWP VMISGSC    FGRGDFQELDQ
Sbjct: 72  SAYGYLTGSPGVLLTVSGPGCVHGLAGVSNAMVNAWPLVMISGSCDQRDFGRGDFQELDQ 131

Query: 151 VEAVKPFSKFSVKATDISEIPNCVARVLNIAVSGRPGGCYIDLPSDILHQTISESEAERL 210
           VEAVKPFSK SVKATDISEIPNCVARVLN AVSGRPGGCY DLPSD+LHQT+SESEAERL
Sbjct: 132 VEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLHQTVSESEAERL 191

Query: 211 LGAAEKFTHREVIYRVPNSQIEAAISLLRRAERPLIVFGKGAAIARAEGPLKKLVESTGI 270
           L AAE+FTHREVI+RVPNSQIE AISLL+ AERPLIVFGKGAAIARAEGPLKKLVESTGI
Sbjct: 192 LAAAEEFTHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEGPLKKLVESTGI 251

Query: 271 PFLPTPMGKGLLPDSHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFI 330
           PFLPTPMGKGLLPD+HELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFI
Sbjct: 252 PFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFI 311

Query: 331 LVDICKEEIELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVEAISQKSKDNVAK 390
           LVDICKEE+ELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVEAISQK+KDNVAK
Sbjct: 312 LVDICKEEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVEAISQKAKDNVAK 371

Query: 391 MEVQLARDVVPFNFLTPMRIIRDAIAALGSPAPILVSEGANTMDVGRSVLIQTEPRTRLD 450
           MEVQLARDVVPFNFLTPMRIIRDAI+ALGSPAPILVSEGANTMDVGRSVL+QTEPRTRLD
Sbjct: 372 MEVQLARDVVPFNFLTPMRIIRDAISALGSPAPILVSEGANTMDVGRSVLVQTEPRTRLD 431

Query: 451 AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLAVVVIVFNNG 510
           AGTWGTMG+GLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQL VVVIVFNNG
Sbjct: 432 AGTWGTMGIGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLPVVVIVFNNG 491

Query: 511 GVYGGDRRTPQEITGPYKHDPAPTSFVPSASYHKMMEAFGGRGYHVETVEELKSALAESF 570
           GVYGGDRRTPQEITGPYKHDPAPTSFVPSASYHKM+EAFGG+GY+VET EELKSALAESF
Sbjct: 492 GVYGGDRRTPQEITGPYKHDPAPTSFVPSASYHKMIEAFGGKGYYVETPEELKSALAESF 551

Query: 571 SARKPAVINVTIDPYAGAESGRLQHKN 597
           SARKPAV+NV IDPYAGAESGRLQHKN
Sbjct: 552 SARKPAVVNVKIDPYAGAESGRLQHKN 578

BLAST of Csor.00g123370 vs. NCBI nr
Match: KAG7036762.1 (2-hydroxyacyl-CoA lyase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 957 bits (2475), Expect = 0.0
Identity = 467/507 (92.11%), Postives = 493/507 (97.24%), Query Frame = 0

Query: 91  SAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPFVMISGSCHSSYFGRGDFQELDQ 150
           SAYGYLTGRPGVLLTVSGPGCVHGLAG+SNAM NAWP VMISGSC    FGRGDFQELDQ
Sbjct: 72  SAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRDFGRGDFQELDQ 131

Query: 151 VEAVKPFSKFSVKATDISEIPNCVARVLNIAVSGRPGGCYIDLPSDILHQTISESEAERL 210
           +EAVKPFSKFSVKATDISEIPNCVARVLN AVSGRPGGCY DLPSD+LHQT++ESEAERL
Sbjct: 132 IEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLHQTVTESEAERL 191

Query: 211 LGAAEKFTHREVIYRVPNSQIEAAISLLRRAERPLIVFGKGAAIARAEGPLKKLVESTGI 270
           L AAE+F+HREVI+RVPNSQIE AISLL+RAERPLIVFGKGAAIARAEGPLKKLVESTGI
Sbjct: 192 LAAAEEFSHREVIHRVPNSQIEEAISLLKRAERPLIVFGKGAAIARAEGPLKKLVESTGI 251

Query: 271 PFLPTPMGKGLLPDSHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFI 330
           PFLPTPMGKGLLPD+H+LAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFI
Sbjct: 252 PFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFI 311

Query: 331 LVDICKEEIELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVEAISQKSKDNVAK 390
           L+DICKEE+ELRKPHLGLIGDAK++LESINKEIKDDPFCLGKSHPWVEAISQK++DNVAK
Sbjct: 312 LIDICKEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEAISQKARDNVAK 371

Query: 391 MEVQLARDVVPFNFLTPMRIIRDAIAALGSPAPILVSEGANTMDVGRSVLIQTEPRTRLD 450
           MEVQLARDVVPFNF+TPMRIIRDAI+A+GSPAPILVSEGANTMDVGRSVL+QTEPRTRLD
Sbjct: 372 MEVQLARDVVPFNFMTPMRIIRDAISAVGSPAPILVSEGANTMDVGRSVLVQTEPRTRLD 431

Query: 451 AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLAVVVIVFNNG 510
           AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQL VVVIVFNNG
Sbjct: 432 AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLPVVVIVFNNG 491

Query: 511 GVYGGDRRTPQEITGPYKHDPAPTSFVPSASYHKMMEAFGGRGYHVETVEELKSALAESF 570
           GVYGGDRRTPQEITGPYKHDPAPTSFVPSA+YHKM+EAFGG+GY+VET EELKSAL+ESF
Sbjct: 492 GVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETPEELKSALSESF 551

Query: 571 SARKPAVINVTIDPYAGAESGRLQHKN 597
           SAR PAVINV IDPYAGAESGRLQHKN
Sbjct: 552 SARSPAVINVKIDPYAGAESGRLQHKN 578

BLAST of Csor.00g123370 vs. ExPASy TrEMBL
Match: A0A6J1GV74 (2-hydroxyacyl-CoA lyase-like OS=Cucurbita moschata OX=3662 GN=LOC111457768 PE=3 SV=1)

HSP 1 Score: 1014 bits (2621), Expect = 0.0
Identity = 506/507 (99.80%), Postives = 506/507 (99.80%), Query Frame = 0

Query: 91  SAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPFVMISGSCHSSYFGRGDFQELDQ 150
           SAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPFVMISGSCHSSYFGRGDFQELDQ
Sbjct: 72  SAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPFVMISGSCHSSYFGRGDFQELDQ 131

Query: 151 VEAVKPFSKFSVKATDISEIPNCVARVLNIAVSGRPGGCYIDLPSDILHQTISESEAERL 210
           VEAVKPFSKFSVKATDISEIPNCVARVLNIAVSGRPGGCYIDLPSDILHQTISESEAERL
Sbjct: 132 VEAVKPFSKFSVKATDISEIPNCVARVLNIAVSGRPGGCYIDLPSDILHQTISESEAERL 191

Query: 211 LGAAEKFTHREVIYRVPNSQIEAAISLLRRAERPLIVFGKGAAIARAEGPLKKLVESTGI 270
           LGAAEKFTHREVIYRVPNSQIEAAISLLRRAERPLIVFGKGAAIARAEGPLKKLVESTGI
Sbjct: 192 LGAAEKFTHREVIYRVPNSQIEAAISLLRRAERPLIVFGKGAAIARAEGPLKKLVESTGI 251

Query: 271 PFLPTPMGKGLLPDSHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFI 330
           PFLPTPMGKGLLPDSHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFI
Sbjct: 252 PFLPTPMGKGLLPDSHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFI 311

Query: 331 LVDICKEEIELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVEAISQKSKDNVAK 390
           LVDICKEEIELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVEAISQKSKDNVAK
Sbjct: 312 LVDICKEEIELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVEAISQKSKDNVAK 371

Query: 391 MEVQLARDVVPFNFLTPMRIIRDAIAALGSPAPILVSEGANTMDVGRSVLIQTEPRTRLD 450
           MEVQLARDVVPFNFLTPMRIIRDAIAALGSPAPILVSEGANTMDVGRSVLIQTEPRTRLD
Sbjct: 372 MEVQLARDVVPFNFLTPMRIIRDAIAALGSPAPILVSEGANTMDVGRSVLIQTEPRTRLD 431

Query: 451 AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLAVVVIVFNNG 510
           AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLAVVVIVFNNG
Sbjct: 432 AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLAVVVIVFNNG 491

Query: 511 GVYGGDRRTPQEITGPYKHDPAPTSFVPSASYHKMMEAFGGRGYHVETVEELKSALAESF 570
           GVYGGDRRTPQEITGPYKHDPAPTSFVPSASYHKMMEAFGGRGYHVET EELKSALAESF
Sbjct: 492 GVYGGDRRTPQEITGPYKHDPAPTSFVPSASYHKMMEAFGGRGYHVETAEELKSALAESF 551

Query: 571 SARKPAVINVTIDPYAGAESGRLQHKN 597
           SARKPAVINVTIDPYAGAESGRLQHKN
Sbjct: 552 SARKPAVINVTIDPYAGAESGRLQHKN 578

BLAST of Csor.00g123370 vs. ExPASy TrEMBL
Match: A0A6J1KDD5 (2-hydroxyacyl-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC111492838 PE=3 SV=1)

HSP 1 Score: 956 bits (2472), Expect = 0.0
Identity = 466/507 (91.91%), Postives = 492/507 (97.04%), Query Frame = 0

Query: 91  SAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPFVMISGSCHSSYFGRGDFQELDQ 150
           SAYGYLTGRPGVLLTVSGPGCVHGLAG+SNAM NAWP VMISGSC    FGRGDFQELDQ
Sbjct: 72  SAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRDFGRGDFQELDQ 131

Query: 151 VEAVKPFSKFSVKATDISEIPNCVARVLNIAVSGRPGGCYIDLPSDILHQTISESEAERL 210
           +EAVKPFSKFSVKATDISEIPNCVARVLN AVSGRPGGCY DLPSD+LHQT++E+EAERL
Sbjct: 132 IEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLHQTVTETEAERL 191

Query: 211 LGAAEKFTHREVIYRVPNSQIEAAISLLRRAERPLIVFGKGAAIARAEGPLKKLVESTGI 270
           L AAE+F+HREVI+RVPNSQIE AISLL+RAERPLIVFGKGAAIARAEGPLKKLVESTGI
Sbjct: 192 LAAAEEFSHREVIHRVPNSQIEEAISLLKRAERPLIVFGKGAAIARAEGPLKKLVESTGI 251

Query: 271 PFLPTPMGKGLLPDSHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFI 330
           PFLPTPMGKGLLPD+H+LAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFI
Sbjct: 252 PFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFI 311

Query: 331 LVDICKEEIELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVEAISQKSKDNVAK 390
           LVDICKEE+E+RKPHLGLIGDAK++LESINKEIKDDPFCLGKSHPWVEAISQK++DNVAK
Sbjct: 312 LVDICKEEVEMRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEAISQKARDNVAK 371

Query: 391 MEVQLARDVVPFNFLTPMRIIRDAIAALGSPAPILVSEGANTMDVGRSVLIQTEPRTRLD 450
           MEVQLARDVVPFNF+TPMRIIRDAI ALGSPAPILVSEGANTMDVGRSVL+QTEPRTRLD
Sbjct: 372 MEVQLARDVVPFNFMTPMRIIRDAITALGSPAPILVSEGANTMDVGRSVLVQTEPRTRLD 431

Query: 451 AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLAVVVIVFNNG 510
           AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQL V+VIVFNNG
Sbjct: 432 AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLPVIVIVFNNG 491

Query: 511 GVYGGDRRTPQEITGPYKHDPAPTSFVPSASYHKMMEAFGGRGYHVETVEELKSALAESF 570
           GVYGGDRRTPQEITGPYKHDPAPTSFVPSA+YHKM+EAFGG+GY+VET EELKSAL+ESF
Sbjct: 492 GVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETPEELKSALSESF 551

Query: 571 SARKPAVINVTIDPYAGAESGRLQHKN 597
           SAR PAVINV IDPYAGAESGRLQHKN
Sbjct: 552 SARSPAVINVKIDPYAGAESGRLQHKN 578

BLAST of Csor.00g123370 vs. ExPASy TrEMBL
Match: A0A6J1GAR5 (2-hydroxyacyl-CoA lyase-like OS=Cucurbita moschata OX=3662 GN=LOC111452318 PE=3 SV=1)

HSP 1 Score: 951 bits (2457), Expect = 0.0
Identity = 464/507 (91.52%), Postives = 490/507 (96.65%), Query Frame = 0

Query: 91  SAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPFVMISGSCHSSYFGRGDFQELDQ 150
           SAYGYLTGRPGVLLTVSGPGCVHGLAG+SNAM NAWP VMISGSC    FGRGDFQELDQ
Sbjct: 72  SAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRDFGRGDFQELDQ 131

Query: 151 VEAVKPFSKFSVKATDISEIPNCVARVLNIAVSGRPGGCYIDLPSDILHQTISESEAERL 210
           +EAVKPFSKFSVKATDISEIPNCVARVLN AVSGRPGGCY DLPSD+LHQT++ESEAE L
Sbjct: 132 IEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLHQTVTESEAEGL 191

Query: 211 LGAAEKFTHREVIYRVPNSQIEAAISLLRRAERPLIVFGKGAAIARAEGPLKKLVESTGI 270
           L AAE+F+HREVI+RVPNSQIE AISLL+ AERPLIVFGKGAAIARAEGP+KK VESTGI
Sbjct: 192 LAAAEEFSHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEGPMKKFVESTGI 251

Query: 271 PFLPTPMGKGLLPDSHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFI 330
           PFLPTPMGKGLLPD+H+LAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFI
Sbjct: 252 PFLPTPMGKGLLPDTHKLAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFI 311

Query: 331 LVDICKEEIELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVEAISQKSKDNVAK 390
           LVDICKEE+ELRKPHLGLIGDAK++LESINKEIKDDPFCLGKSHPWVEAISQK++DNVAK
Sbjct: 312 LVDICKEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEAISQKARDNVAK 371

Query: 391 MEVQLARDVVPFNFLTPMRIIRDAIAALGSPAPILVSEGANTMDVGRSVLIQTEPRTRLD 450
           MEVQLARDVVPFNF+TPMRIIRDAI+A+GSPAPILVSEGANTMDVGRSVL+QTEPRTRLD
Sbjct: 372 MEVQLARDVVPFNFMTPMRIIRDAISAVGSPAPILVSEGANTMDVGRSVLVQTEPRTRLD 431

Query: 451 AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLAVVVIVFNNG 510
           AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQL VVVIVFNNG
Sbjct: 432 AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLPVVVIVFNNG 491

Query: 511 GVYGGDRRTPQEITGPYKHDPAPTSFVPSASYHKMMEAFGGRGYHVETVEELKSALAESF 570
           GVYGGDRRTPQEITGPYKHDPAPTSFVPSA+YHKM+EAFGG+GY+VET EELKSAL+ESF
Sbjct: 492 GVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETPEELKSALSESF 551

Query: 571 SARKPAVINVTIDPYAGAESGRLQHKN 597
           SAR PAVINV IDPYAGAESGRLQHKN
Sbjct: 552 SARSPAVINVKIDPYAGAESGRLQHKN 578

BLAST of Csor.00g123370 vs. ExPASy TrEMBL
Match: A0A5A7VDZ6 (2-hydroxyacyl-CoA lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2030G00330 PE=3 SV=1)

HSP 1 Score: 947 bits (2447), Expect = 0.0
Identity = 470/507 (92.70%), Postives = 487/507 (96.06%), Query Frame = 0

Query: 91  SAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPFVMISGSCHSSYFGRGDFQELDQ 150
           SAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWP VMISGSC    FGRGDFQELDQ
Sbjct: 72  SAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRDFGRGDFQELDQ 131

Query: 151 VEAVKPFSKFSVKATDISEIPNCVARVLNIAVSGRPGGCYIDLPSDILHQTISESEAERL 210
           VEAVKPFSK SVKATDISEIPNCVARVLN AVSGRPGGCY DLPSD+LHQTISESEAERL
Sbjct: 132 VEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLHQTISESEAERL 191

Query: 211 LGAAEKFTHREVIYRVPNSQIEAAISLLRRAERPLIVFGKGAAIARAEGPLKKLVESTGI 270
           L AAE+F +REVI RVPNSQIE AISLL+ AERPLIVFGKGAAIARAEGPLKKLVE+TGI
Sbjct: 192 LAAAEEFANREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEGPLKKLVETTGI 251

Query: 271 PFLPTPMGKGLLPDSHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFI 330
           PFLPTPMGKGLLPD+HELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFI
Sbjct: 252 PFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFI 311

Query: 331 LVDICKEEIELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVEAISQKSKDNVAK 390
           LVDICKEE+ELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWV AISQK+KDNVAK
Sbjct: 312 LVDICKEEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVGAISQKAKDNVAK 371

Query: 391 MEVQLARDVVPFNFLTPMRIIRDAIAALGSPAPILVSEGANTMDVGRSVLIQTEPRTRLD 450
           MEVQLA+DVVPFNFLTPMRIIRDAI A+GSPAPILVSEGANTMDVGRSVL+QTEPRTRLD
Sbjct: 372 MEVQLAKDVVPFNFLTPMRIIRDAILAVGSPAPILVSEGANTMDVGRSVLVQTEPRTRLD 431

Query: 451 AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLAVVVIVFNNG 510
           AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRY+L VVVIVFNN 
Sbjct: 432 AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYELPVVVIVFNNS 491

Query: 511 GVYGGDRRTPQEITGPYKHDPAPTSFVPSASYHKMMEAFGGRGYHVETVEELKSALAESF 570
           GVYGGDRRTPQEITGPYKHDPAPTSFVPSASY KM+EAFGG+GY+VE+ EELKSALAESF
Sbjct: 492 GVYGGDRRTPQEITGPYKHDPAPTSFVPSASYDKMIEAFGGKGYYVESPEELKSALAESF 551

Query: 571 SARKPAVINVTIDPYAGAESGRLQHKN 597
           SARKPAV+NV IDPYAGAESGRLQHKN
Sbjct: 552 SARKPAVVNVKIDPYAGAESGRLQHKN 578

BLAST of Csor.00g123370 vs. ExPASy TrEMBL
Match: A0A1S3B9H9 (2-hydroxyacyl-CoA lyase OS=Cucumis melo OX=3656 GN=LOC103487508 PE=3 SV=1)

HSP 1 Score: 947 bits (2447), Expect = 0.0
Identity = 470/507 (92.70%), Postives = 487/507 (96.06%), Query Frame = 0

Query: 91  SAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPFVMISGSCHSSYFGRGDFQELDQ 150
           SAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWP VMISGSC    FGRGDFQELDQ
Sbjct: 72  SAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRDFGRGDFQELDQ 131

Query: 151 VEAVKPFSKFSVKATDISEIPNCVARVLNIAVSGRPGGCYIDLPSDILHQTISESEAERL 210
           VEAVKPFSK SVKATDISEIPNCVARVLN AVSGRPGGCY DLPSD+LHQTISESEAERL
Sbjct: 132 VEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLHQTISESEAERL 191

Query: 211 LGAAEKFTHREVIYRVPNSQIEAAISLLRRAERPLIVFGKGAAIARAEGPLKKLVESTGI 270
           L AAE+F +REVI RVPNSQIE AISLL+ AERPLIVFGKGAAIARAEGPLKKLVE+TGI
Sbjct: 192 LAAAEEFANREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEGPLKKLVETTGI 251

Query: 271 PFLPTPMGKGLLPDSHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFI 330
           PFLPTPMGKGLLPD+HELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFI
Sbjct: 252 PFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFI 311

Query: 331 LVDICKEEIELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVEAISQKSKDNVAK 390
           LVDICKEE+ELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWV AISQK+KDNVAK
Sbjct: 312 LVDICKEEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVGAISQKAKDNVAK 371

Query: 391 MEVQLARDVVPFNFLTPMRIIRDAIAALGSPAPILVSEGANTMDVGRSVLIQTEPRTRLD 450
           MEVQLA+DVVPFNFLTPMRIIRDAI A+GSPAPILVSEGANTMDVGRSVL+QTEPRTRLD
Sbjct: 372 MEVQLAKDVVPFNFLTPMRIIRDAILAVGSPAPILVSEGANTMDVGRSVLVQTEPRTRLD 431

Query: 451 AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLAVVVIVFNNG 510
           AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRY+L VVVIVFNN 
Sbjct: 432 AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYELPVVVIVFNNS 491

Query: 511 GVYGGDRRTPQEITGPYKHDPAPTSFVPSASYHKMMEAFGGRGYHVETVEELKSALAESF 570
           GVYGGDRRTPQEITGPYKHDPAPTSFVPSASY KM+EAFGG+GY+VE+ EELKSALAESF
Sbjct: 492 GVYGGDRRTPQEITGPYKHDPAPTSFVPSASYDKMIEAFGGKGYYVESPEELKSALAESF 551

Query: 571 SARKPAVINVTIDPYAGAESGRLQHKN 597
           SARKPAV+NV IDPYAGAESGRLQHKN
Sbjct: 552 SARKPAVVNVKIDPYAGAESGRLQHKN 578

BLAST of Csor.00g123370 vs. TAIR 10
Match: AT5G17380.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 824.7 bits (2129), Expect = 4.8e-239
Identity = 399/507 (78.70%), Postives = 453/507 (89.35%), Query Frame = 0

Query: 91  SAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPFVMISGSCHSSYFGRGDFQELDQ 150
           SAYGYLTG+PG+LLTVSGPGCVHGLAG+SNA VN WP VMISGSC     GRGDFQELDQ
Sbjct: 66  SAYGYLTGKPGILLTVSGPGCVHGLAGLSNAWVNTWPMVMISGSCDQRDVGRGDFQELDQ 125

Query: 151 VEAVKPFSKFSVKATDISEIPNCVARVLNIAVSGRPGGCYIDLPSDILHQTISESEAERL 210
           +EAVK FSK S KA D+ EIP+CV+RVL+ AVSGRPGGCY+D+P+D+L Q ISESEA++L
Sbjct: 126 IEAVKAFSKLSEKAKDVREIPDCVSRVLDRAVSGRPGGCYLDIPTDVLRQKISESEADKL 185

Query: 211 LGAAEKFTHREVIYRVPNSQIEAAISLLRRAERPLIVFGKGAAIARAEGPLKKLVESTGI 270
           +   E+    E I     S+IE+A+SLLR+AERPLIVFGKGAA +RAE  LKKLVE TGI
Sbjct: 186 VDEVERSRKEEPIRGSLRSEIESAVSLLRKAERPLIVFGKGAAYSRAEDELKKLVEITGI 245

Query: 271 PFLPTPMGKGLLPDSHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFI 330
           PFLPTPMGKGLLPD+HE +ATAARSLAIGKCDVALVVGARLNWLLHFGE PKW KDVKFI
Sbjct: 246 PFLPTPMGKGLLPDTHEFSATAARSLAIGKCDVALVVGARLNWLLHFGESPKWDKDVKFI 305

Query: 331 LVDICKEEIELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVEAISQKSKDNVAK 390
           LVD+ +EEIELRKPHLG++GDAK V+  +N+EIKDDPFCLGKS+ WVE+IS+K+K+N  K
Sbjct: 306 LVDVSEEEIELRKPHLGIVGDAKTVIGLLNREIKDDPFCLGKSNSWVESISKKAKENGEK 365

Query: 391 MEVQLARDVVPFNFLTPMRIIRDAIAALGSPAPILVSEGANTMDVGRSVLIQTEPRTRLD 450
           ME+QLA+DVVPFNFLTPMRIIRDAI A+  P+P++VSEGANTMDVGRSVL+Q EPRTRLD
Sbjct: 366 MEIQLAKDVVPFNFLTPMRIIRDAILAVEGPSPVVVSEGANTMDVGRSVLVQKEPRTRLD 425

Query: 451 AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLAVVVIVFNNG 510
           AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRY LAVV+IVFNNG
Sbjct: 426 AGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYNLAVVIIVFNNG 485

Query: 511 GVYGGDRRTPQEITGPYKHDPAPTSFVPSASYHKMMEAFGGRGYHVETVEELKSALAESF 570
           GVYGGDRR P+EI+GP+K DPAPTSFVP+A YHK++EAFGG+GY VET +ELKSALAESF
Sbjct: 486 GVYGGDRRGPEEISGPHKEDPAPTSFVPNAGYHKLIEAFGGKGYIVETPDELKSALAESF 545

Query: 571 SARKPAVINVTIDPYAGAESGRLQHKN 598
           +ARKPAV+NV IDP+AGAESGRLQHKN
Sbjct: 546 AARKPAVVNVIIDPFAGAESGRLQHKN 572

BLAST of Csor.00g123370 vs. TAIR 10
Match: AT3G48560.1 (chlorsulfuron/imidazolinone resistant 1 )

HSP 1 Score: 159.1 bits (401), Expect = 1.1e-38
Identity = 121/433 (27.94%), Postives = 198/433 (45.73%), Query Frame = 0

Query: 93  YGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPFVMISGSCHSSYFGRGDFQELDQVE 152
           Y   +G+PG+ +  SGPG  + ++G+++A++++ P V I+G       G   FQE   VE
Sbjct: 154 YARSSGKPGICIATSGPGATNLVSGLADALLDSVPLVAITGQVPRRMIGTDAFQETPIVE 213

Query: 153 AVKPFSKFSVKATDISEIPNCVARVLNIAVSGRPGGCYIDLPSDILHQTI--SESEAERL 212
             +  +K +    D+ +IP  +     +A SGRPG   +D+P DI  Q    +  +A RL
Sbjct: 214 VTRSITKHNYLVMDVEDIPRIIEEAFFLATSGRPGPVLVDVPKDIQQQLAIPNWEQAMRL 273

Query: 213 LGAAEKFTHREVIYRVPNSQIEAAISLLRRAERPLIVFGKGAAIARAEGPLKKLVESTGI 272
            G   +           +S +E  + L+  +++P++  G G   +  E  L + VE TGI
Sbjct: 274 PGYMSRMPKPP-----EDSHLEQIVRLISESKKPVLYVGGGCLNSSDE--LGRFVELTGI 333

Query: 273 PFLPTPMGKGLLPDSHELA-------ATAARSLAIGKCDVALVVGARLNWLLHFGEPPKW 332
           P   T MG G  P   EL+        T   + A+   D+ L  G R +  +  G+   +
Sbjct: 334 PVASTLMGLGSYPCDDELSLHMLGMHGTVYANYAVEHSDLLLAFGVRFDDRV-TGKLEAF 393

Query: 333 SKDVKFILVDICKEEIELRK-PHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVEAISQ 392
           +   K + +DI   EI   K PH+ + GD K  L+ +NK +++             A   
Sbjct: 394 ASRAKIVHIDIDSAEIGKNKTPHVSVCGDVKLALQGMNKVLEN------------RAEEL 453

Query: 393 KSKDNVAKMEVQLARDVVPFNFLT------PMRIIRDAIAALGSPAPILVSEGANTMDVG 452
           K    V + E+ + +   P +F T      P   I+         A I    G + M   
Sbjct: 454 KLDFGVWRNELNVQKQKFPLSFKTFGEAIPPQYAIKVLDELTDGKAIISTGVGQHQMWAA 513

Query: 453 RSVLIQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLV 510
           +    + +PR  L +G  G MG GL   I A+VA+PD +VV ++GD  F  +  E+ T+ 
Sbjct: 514 QFYNYK-KPRQWLSSGGLGAMGFGLPAAIGASVANPDAIVVDIDGDGSFIMNVQELATIR 565

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LF466.7e-23878.702-hydroxyacyl-CoA lyase OS=Arabidopsis thaliana OX=3702 GN=HACL PE=1 SV=1[more]
Q0JMH03.9e-23075.542-hydroxyacyl-CoA lyase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0505400... [more]
Q8CHM75.7e-11243.422-hydroxyacyl-CoA lyase 1 OS=Rattus norvegicus OX=10116 GN=Hacl1 PE=1 SV=1[more]
P0AFI17.4e-11242.35Oxalyl-CoA decarboxylase OS=Escherichia coli O157:H7 OX=83334 GN=oxc PE=3 SV=1[more]
P0AFI07.4e-11242.35Oxalyl-CoA decarboxylase OS=Escherichia coli (strain K12) OX=83333 GN=oxc PE=1 S... [more]
Match NameE-valueIdentityDescription
KAG6581738.10.0100.002-hydroxyacyl-CoA lyase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022955931.10.099.802-hydroxyacyl-CoA lyase-like [Cucurbita moschata][more]
XP_023526990.10.097.832-hydroxyacyl-CoA lyase-like [Cucurbita pepo subsp. pepo][more]
XP_038893948.10.093.892-hydroxyacyl-CoA lyase [Benincasa hispida][more]
KAG7036762.10.092.112-hydroxyacyl-CoA lyase [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
A0A6J1GV740.099.802-hydroxyacyl-CoA lyase-like OS=Cucurbita moschata OX=3662 GN=LOC111457768 PE=3 ... [more]
A0A6J1KDD50.091.912-hydroxyacyl-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC111492838 PE=3 SV=1[more]
A0A6J1GAR50.091.522-hydroxyacyl-CoA lyase-like OS=Cucurbita moschata OX=3662 GN=LOC111452318 PE=3 ... [more]
A0A5A7VDZ60.092.702-hydroxyacyl-CoA lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3B9H90.092.702-hydroxyacyl-CoA lyase OS=Cucumis melo OX=3656 GN=LOC103487508 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G17380.14.8e-23978.70Thiamine pyrophosphate dependent pyruvate decarboxylase family protein [more]
AT3G48560.11.1e-3827.94chlorsulfuron/imidazolinone resistant 1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011766Thiamine pyrophosphate enzyme, C-terminal TPP-bindingPFAMPF02775TPP_enzyme_Ccoord: 431..580
e-value: 6.4E-27
score: 94.2
IPR012000Thiamine pyrophosphate enzyme, central domainPFAMPF00205TPP_enzyme_Mcoord: 231..358
e-value: 1.3E-32
score: 112.3
NoneNo IPR availableGENE3D3.40.50.970coord: 383..597
e-value: 8.3E-63
score: 213.3
NoneNo IPR availableGENE3D3.40.50.970coord: 84..209
e-value: 7.5E-28
score: 99.1
NoneNo IPR availableGENE3D3.40.50.1220coord: 227..381
e-value: 5.1E-38
score: 132.2
NoneNo IPR availablePANTHERPTHR43710:SF22-HYDROXYACYL-COA LYASE 1coord: 91..597
NoneNo IPR availableCDDcd02004TPP_BZL_OCoD_HPCLcoord: 404..583
e-value: 7.38997E-82
score: 252.453
NoneNo IPR availableCDDcd07035TPP_PYR_POX_likecoord: 88..194
e-value: 2.12197E-30
score: 114.551
IPR012001Thiamine pyrophosphate enzyme, N-terminal TPP-binding domainPFAMPF02776TPP_enzyme_Ncoord: 88..204
e-value: 2.2E-26
score: 92.5
IPR045025TPP-binding domain containing protein HACL1-likePANTHERPTHR437102-HYDROXYACYL-COA LYASEcoord: 91..597
IPR000399TPP-binding enzyme, conserved sitePROSITEPS00187TPP_ENZYMEScoord: 465..484
IPR029035DHS-like NAD/FAD-binding domain superfamilySUPERFAMILY52467DHS-like NAD/FAD-binding domaincoord: 227..385
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 87..204
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 407..586

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g123370.m01Csor.00g123370.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001561 fatty acid alpha-oxidation
cellular_component GO:0005777 peroxisome
molecular_function GO:0016829 lyase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0030976 thiamine pyrophosphate binding
molecular_function GO:0003824 catalytic activity