Csor.00g121400 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g121400
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	Folylpolyglutamate synthase
Location	Csor_Chr04: 19690899 .. 19695410 (+)
RNA-Seq Expression	Csor.00g121400
Synteny	Csor.00g121400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCACTCTCTTTCTCTAGCCGCCCCCTTCCAGTCTTCGGCACTCGCCCTCGGCTTCTCCGACGTTCCCGAAAGGAAGAGATTGGTGAAAAGGTGAGGACCCTTTTCCCCTTTTGACTCTGTTCTTATCTGAGCACTGAATCCTTGGTTATGGAATCTGTAATTATGTTTTGTTGAATATTACTCTGCTGCTTGTACGAGAAGGCGATGAAAATTGAACCCGTTTTTTCTTTATTGCCAATTAGTGTAAGGGAAACGGGGAAATTAGATACCCAATCGAATATATTCTTAATTAGAGCAGGGAATAAGCAGGGAATAAGCTAGACTATGAATGTAATATGTGTAAAGAAATTCTACCTCATCTTAAGCGATTAGGTAGCTTTTTTTTAGAGGGGGTTTGTTCCGTGAGCTATATGTTGGAACTTCCTGATTGTTTGATGTATTCTATGCTTTTCCCTGCATCTAAAAGACATTAGCTGTTTTGCAGCTTTGGAGAGTAGCAAACGTTGGGGGCCTTTGAATCTTTTAGACTAGGCTGAATCCTCAGGATATTGGAGAAGCAGGGGGTCATCTAGTAACCATGGAGGAAGGTATGGACACATTTTAAACCACTTTTGATGTGTTAAGCTATTCTATCATGTAAAACCAAAAAACGTTTTGGGGTTGTGATGGGTGCTTTTGAAGGTATGGGAGCATTGAAATACTCGCGGACAGCCTATGAAGAGGCATTGGATGTACTGTCCTCGTTAATTGTCAGCCGTAGACGCTGCGATAAATCTGGAGATCGGTTTGAGTTGCTGTTTGAGTATGTAAAGGTATTACTGAAACTTAACACTTTATTATTGCTATAGTATACTTGAGCTGATGATCATTGAGTTGACTCAATATAATAGATGCTTGACTTAGAGGAGTCCATGTCGAAAATGAAGGTCATCCATGTAGCTGGCACCAAAGGCAAGGTACCATCTTCATCAGTTCATGCTCTTTCATTTGGATACTTCTACTTTTGTCCCATTCACTTTAACATTGTCAACTTAGAATTTTCGAACTTTGCTTAGAAAACATCATTGAGTTAGCTTAAAAATGATGCTGTGTTAACTAAACTTTTTTCTTTTTTCGGGACTTACATAGGGATCCACGTGCACCTTTACCGAATCCATATTGCGTAAATGTGGCTTCCACACTGGCCTTTTCACTTCTCCTCACCTTATTGATGTCCGAGAAAGATTTCGCTTGGATGGGTAACTATTGTGCATACTACGTAGTTTTGTTGTTCTTTTACGTGGTAAAGTTGGTCGATTGCTACTTTGTTTATTGAGATGATGTAGATGGTTGTCCTTACAGTAAATCCATTGATTTTTCGTTGTATCAACGACTTGCTTCGTATTATCTAACTTTATTTCTGGAACTATTTTCTCTCTTCCCACTTTGAAGTTGCAAACTCTTGAATACCTTCTAATTGGTTGTTGCAGCATCGACATCAGTGAAGAGAAGTTTTTAGCCTATTTCTGGTGGTGTTATAATAGGCTAAAGGTACAATTTGATGTGTTATTATTACTTTTAATACATAAGTTCATCTAATGGATAAAATATATGGTGTCTTTAAATCTAATTGTATATGTAAACATTATATTTAGATTCTGAGTTGCTTATTCTTGTAGTAAAGAGACGTTTGAACTCTATAAAACTAAACTATGGGATTCATTATTATCTTTTAACTGTAAATAAAGCATTTCTTCGATATTTTGTAAGTTTTCTAGAACAATTTATTTTCTTCAGGAAAAAACTAGTGAGGATGTACCGATGCCTCACTTGTTTCGCTTCCTAGCGTTACTTGCGTTCAAAATTTTTGCAGATGAGCAGGTTTGATGTGTTCTTTTTATTTTAATCTTTTTCTGGATACGCTTTCTTTGGTCAATTTATTACTGGGATTATAATGATGATGTTTTTATTGGCATCAATGAAGGTGGATGTAGCTATCTTGGAGGTTGGTTTAGGTGGAACTTTTGATGCTACCAATGTGGTATGTTCATTCTCTTTTGGCTTAAATTATAAAGCATTTCATGGCATTTCAGATATTGTGAGATCCCACATCGGTTGGAGAGGGGAACGAAGCATTCCTTATAAGGGTGTGGAAACCTTTCCCTAACAGACGCGATTTAAAACTGTGAGACTGATGGTGATACGTAACGGCCCAAAGTGGATAATATTTGCTAGCGGTGGGCTTGGGCTGTTACAGATATAATTTATTCTTCATTAGAAAGTAGAAATCTTCATCAAGGTATAGCCATTTCATAGATTCAAATGTTTCAGGTCCAGGCACCCATTGTGTGTGGTATAGCTTCTTTGGGTTACGACCACACGGAACTTCTTGGTTAGCCTTCTCTCGACCTTGACAATAGTTTTCCTGGAAAATAAATGTTCGACAACTGTTTGCGTTAATGGCTTAAGCACGAGCGTAGATGTTTTCTATCCTGTTTGATAAGTTTGATAATGTCAAGGTTTTCTTACATTGTTGCTTTCTTTCTGTTTTGAATTTGTCCAATTAGGAAACACTCTTGGTGCGATAGCTTCGGAGAAAGCTGGTATCTTCAAGGTTTGCCTCAAGAAACTTTCCTTAAAGCATGAACGTCTAGGAATAATCATTTCATTTTGTTTCCATCCTTTCACTTTGGTGCAGCGTGGGGCTCCAGCTTTTACTGTATGTCAACCTGATGAAGCCATGCATGTGCTTAGAGATAAAGCTTCCCAGTTGGATGTATGTGCAAAGCTCTTCCGTTAGCTACTTCATGACTATGTGTTGTTCACACTGCCTTATATTTGCACGTCCCAGGTACAACTTACAGTAGTGCCATCGTTGGATCCGGAGCTTCTACAAGGTCATAAACTCGGGCTTGAAGGCGAGCACCAGTATCTAAATGCTCGTCTTGCTATTGCGCTATCCTCCACTTGGCTTCACAGGACCGGCCATTCCAATCTGTCGTACAAGGAACAGAGTGTAAGTTCTACATATGGGATTTGTCATCAGTTTTCCGTAAACATATCTCGTGTTTTCTGATTCTTTTTCTTCTTAACAATATGTTACCAGGGGTCTTTGCCTGAGCACTTCATAGAAGGGCTTACCACTGCCAGCTTTGAAGGGCGAGCTCAGATTGTTCCTGACAGATATAATGATATTGAATCCCCGGAAGATCTTGTCTTTTATTTGGATGGTGCCCATAGTCCTGAGAGCATGGTGGTTTGCAGTAGCTGGTTTTGTGCTGCAATAAAACATAACCAGGAAGGGATCTTAGATCATCAGTTGGATGATACTGGTACATCTTCAAATAAACTTCTTCAAGGATATGATAATGATGAGGCAAGGAAGAAATCTGTGCAGGTAGATTTCGACTTTAGCTCGACTTCTCCAAGCTTGTTTTATTGTTTGTCAAGAAAAAGGGATCATAAATAAGAAAGCTAGTTTCAGTTTGAATACCTTTTCATTTATGCAGTGCTTCAACTTCTTTAGATGTTGTTTAATCTAGTAGTGTTGGACATTGTAGGTGCTATTGTTCAATTGTATGTCGGTACGAGATCCACAATTGCTTCTTCAACCTCTGATCAAAACGTGCGCAAATAATGGTATGTATTTGTCGAACTCTTCGAACTGAAAAAATCTGGTGATCTGGTTAATACACAGTATTTTTTTAGGATTTAAAAGAATGATCTAAATATAACATTACTCGGAAAATCGAAGCTATAGAATAAGTTTTTAGCTGATGGATTAACGTCTTACGTTGTGAGATCTCATATTGGTTAGAGAGGGGAACAAAGCATTCCTTATAAGGATGTGGAAACTTCTCCCTAACAGATGCGTTTTAAAACCGTGAGGCTGACAGCGATACGTAATGGTTCAAAGTGGACAATATCTATTAGCATTGGGCTTGGATGTAACGTTAAATAAGATATGCTAGAAATCAATAAAATTCCGTAAAAGTTCGGTTATATGAACTCAGGGGTCCACTTCAAGAAGGCAGTCTTTGTTCCAAATACATCATTGTTCAACAAGGTTGGAACCCATGGTTTAGCAGAAGCAAATGGTGAAGTAGACCTTTCTTGGCAGTTCCATGTTCGAAGGGTGTGGGAAAACATCATCCAATCTGAGAAAGGTATTATCTGTTTATGCTAGTGATCTTTTTCTAAGTCTTTGGAACCATTCTTTATCCTTCCACATATTAGGAGGCGATTCTCAAGGCATTGGTGCCATGGTTGAAGAAGAAAGTGAAAAACCAGAAGTGAGTGGTAAGAGCTGCGAGAACAGCGCAGTGTTTTCTTCTCTCCCAATGGCCATTAAATGGCTCCGAGATAGTGTTCTGCAGCAGAACAGGTCCATTCGACTTCAGGTGCGAATATTTTCGAGTATGTAGACATGGGCACATTTGGAACGAAGAAATTTACGAGCTTTCTATTATTCAGGTTCTTGTGACTGGTTCTTTACATCTTGTGGGAGATGTGTTGAAATTAATCAAGTGA

mRNA sequence

ATGCCACTCTCTTTCTCTAGCCGCCCCCTTCCAGTCTTCGGCACTCGCCCTCGGCTTCTCCGACGTTCCCGAAAGGAAGAGATTGGTGAAAAGACTAGGCTGAATCCTCAGGATATTGGAGAAGCAGGGGGTCATCTAGTAACCATGGAGGAAGGTATGGGAGCATTGAAATACTCGCGGACAGCCTATGAAGAGGCATTGGATGTACTGTCCTCGTTAATTGTCAGCCGTAGACGCTGCGATAAATCTGGAGATCGGTTTGAGTTGCTGTTTGAGTATGTAAAGATGCTTGACTTAGAGGAGTCCATGTCGAAAATGAAGGTCATCCATGTAGCTGGCACCAAAGGCAAGGGATCCACGTGCACCTTTACCGAATCCATATTGCGTAAATGTGGCTTCCACACTGGCCTTTTCACTTCTCCTCACCTTATTGATGTCCGAGAAAGATTTCGCTTGGATGGCATCGACATCAGTGAAGAGAAGTTTTTAGCCTATTTCTGGTGGTGTTATAATAGGCTAAAGGAAAAAACTAGTGAGGATGTACCGATGCCTCACTTGTTTCGCTTCCTAGCGTTACTTGCGTTCAAAATTTTTGCAGATGAGCAGGTGGATGTAGCTATCTTGGAGGTTGGTTTAGGTGGAACTTTTGATGCTACCAATGTGGTCCAGGCACCCATTGTGTGTGGTATAGCTTCTTTGGGTTACGACCACACGGAACTTCTTGGAAACACTCTTGGTGCGATAGCTTCGGAGAAAGCTGGTATCTTCAAGCGTGGGGCTCCAGCTTTTACTGTATGTCAACCTGATGAAGCCATGCATGTGCTTAGAGATAAAGCTTCCCAGTTGGATGTACAACTTACAGTAGTGCCATCGTTGGATCCGGAGCTTCTACAAGGTCATAAACTCGGGCTTGAAGGCGAGCACCAGTATCTAAATGCTCGTCTTGCTATTGCGCTATCCTCCACTTGGCTTCACAGGACCGGCCATTCCAATCTGTCGTACAAGGAACAGAGTGGGTCTTTGCCTGAGCACTTCATAGAAGGGCTTACCACTGCCAGCTTTGAAGGGCGAGCTCAGATTGTTCCTGACAGATATAATGATATTGAATCCCCGGAAGATCTTGTCTTTTATTTGGATGGTGCCCATAGTCCTGAGAGCATGGTGGTTTGCAGTAGCTGGTTTTGTGCTGCAATAAAACATAACCAGGAAGGGATCTTAGATCATCAGTTGGATGATACTGGTACATCTTCAAATAAACTTCTTCAAGGATATGATAATGATGAGGCAAGGAAGAAATCTGTGCAGGTGCTATTGTTCAATTGTATGTCGGTACGAGATCCACAATTGCTTCTTCAACCTCTGATCAAAACGTGCGCAAATAATGGGGTCCACTTCAAGAAGGCAGTCTTTGTTCCAAATACATCATTGTTCAACAAGGTTGGAACCCATGGTTTAGCAGAAGCAAATGGTGAAGTAGACCTTTCTTGGCAGTTCCATGTTCGAAGGGTGTGGGAAAACATCATCCAATCTGAGAAAGGAGGCGATTCTCAAGGCATTGGTGCCATGGTTGAAGAAGAAAGTGAAAAACCAGAAGTGAGTGGTAAGAGCTGCGAGAACAGCGCAGTGTTTTCTTCTCTCCCAATGGCCATTAAATGGCTCCGAGATAGTGTTCTGCAGCAGAACAGGTCCATTCGACTTCAGGTTCTTGTGACTGGTTCTTTACATCTTGTGGGAGATGTGTTGAAATTAATCAAGTGA

Coding sequence (CDS)

ATGCCACTCTCTTTCTCTAGCCGCCCCCTTCCAGTCTTCGGCACTCGCCCTCGGCTTCTCCGACGTTCCCGAAAGGAAGAGATTGGTGAAAAGACTAGGCTGAATCCTCAGGATATTGGAGAAGCAGGGGGTCATCTAGTAACCATGGAGGAAGGTATGGGAGCATTGAAATACTCGCGGACAGCCTATGAAGAGGCATTGGATGTACTGTCCTCGTTAATTGTCAGCCGTAGACGCTGCGATAAATCTGGAGATCGGTTTGAGTTGCTGTTTGAGTATGTAAAGATGCTTGACTTAGAGGAGTCCATGTCGAAAATGAAGGTCATCCATGTAGCTGGCACCAAAGGCAAGGGATCCACGTGCACCTTTACCGAATCCATATTGCGTAAATGTGGCTTCCACACTGGCCTTTTCACTTCTCCTCACCTTATTGATGTCCGAGAAAGATTTCGCTTGGATGGCATCGACATCAGTGAAGAGAAGTTTTTAGCCTATTTCTGGTGGTGTTATAATAGGCTAAAGGAAAAAACTAGTGAGGATGTACCGATGCCTCACTTGTTTCGCTTCCTAGCGTTACTTGCGTTCAAAATTTTTGCAGATGAGCAGGTGGATGTAGCTATCTTGGAGGTTGGTTTAGGTGGAACTTTTGATGCTACCAATGTGGTCCAGGCACCCATTGTGTGTGGTATAGCTTCTTTGGGTTACGACCACACGGAACTTCTTGGAAACACTCTTGGTGCGATAGCTTCGGAGAAAGCTGGTATCTTCAAGCGTGGGGCTCCAGCTTTTACTGTATGTCAACCTGATGAAGCCATGCATGTGCTTAGAGATAAAGCTTCCCAGTTGGATGTACAACTTACAGTAGTGCCATCGTTGGATCCGGAGCTTCTACAAGGTCATAAACTCGGGCTTGAAGGCGAGCACCAGTATCTAAATGCTCGTCTTGCTATTGCGCTATCCTCCACTTGGCTTCACAGGACCGGCCATTCCAATCTGTCGTACAAGGAACAGAGTGGGTCTTTGCCTGAGCACTTCATAGAAGGGCTTACCACTGCCAGCTTTGAAGGGCGAGCTCAGATTGTTCCTGACAGATATAATGATATTGAATCCCCGGAAGATCTTGTCTTTTATTTGGATGGTGCCCATAGTCCTGAGAGCATGGTGGTTTGCAGTAGCTGGTTTTGTGCTGCAATAAAACATAACCAGGAAGGGATCTTAGATCATCAGTTGGATGATACTGGTACATCTTCAAATAAACTTCTTCAAGGATATGATAATGATGAGGCAAGGAAGAAATCTGTGCAGGTGCTATTGTTCAATTGTATGTCGGTACGAGATCCACAATTGCTTCTTCAACCTCTGATCAAAACGTGCGCAAATAATGGGGTCCACTTCAAGAAGGCAGTCTTTGTTCCAAATACATCATTGTTCAACAAGGTTGGAACCCATGGTTTAGCAGAAGCAAATGGTGAAGTAGACCTTTCTTGGCAGTTCCATGTTCGAAGGGTGTGGGAAAACATCATCCAATCTGAGAAAGGAGGCGATTCTCAAGGCATTGGTGCCATGGTTGAAGAAGAAAGTGAAAAACCAGAAGTGAGTGGTAAGAGCTGCGAGAACAGCGCAGTGTTTTCTTCTCTCCCAATGGCCATTAAATGGCTCCGAGATAGTGTTCTGCAGCAGAACAGGTCCATTCGACTTCAGGTTCTTGTGACTGGTTCTTTACATCTTGTGGGAGATGTGTTGAAATTAATCAAGTGA

Protein sequence

MPLSFSSRPLPVFGTRPRLLRRSRKEEIGEKTRLNPQDIGEAGGHLVTMEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKVIHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWWCYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDHQLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKAVFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESEKPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK

Homology

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BLAST of Csor.00g121400 vs. ExPASy Swiss-Prot
Match: F4K2A1 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS1 PE=1 SV=1)

HSP 1 Score: 636.7 bits (1641), Expect = 2.5e-181
Identity = 330/528 (62.50%), Postives = 395/528 (74.81%), Query Frame = 0


Query: 62  AYEEALDVLSSLIVSRRRCDKS--GDRFELLFEYVKMLDLEESMSKMKVIHVAGTKGKGS 121
           +YEEAL  LSSLI  R R DKS  GDRFEL+F+Y+K+LDLEE + KM VIHVAGTKGKGS
Sbjct: 66  SYEEALAALSSLITKRSRADKSNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKGKGS 125

Query: 122 TCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWWCYNRLKEKTSE 181
           TCTFTESI+R  GF TGLFTSPHLIDVRERFRLDG+DISEEKFL YFWWCYNRLKE+T+E
Sbjct: 126 TCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKERTNE 185

Query: 182 DVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYDHTE 241
           ++PMP  FRFLALLAFKIFA E+VD AILEVGLGG FDATN VQ P+VCGI+SLGYDH E
Sbjct: 186 EIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYDHME 245

Query: 242 LLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTVVPSLDPELLQG 301
           +LG+TLG IA EKAGIFK G PAFTV QPDEAM VL +KAS+ +V L VV  L   LL G
Sbjct: 246 ILGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASETEVNLEVVQPLTARLLSG 305

Query: 302 HKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEGLTTASFEGRAQ 361
            KLGL+GEHQY+NA LA++L+S WL + G   +  + Q   LPE FI+GL TAS +GRAQ
Sbjct: 306 QKLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVPSRTQMSILPEKFIKGLATASLQGRAQ 365

Query: 362 IVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIK-HNQEGILDHQLDDTGTSSN 421
           +VPD+Y +  +  DLVFYLDGAHSPESM  C+ WF  A+K  NQ G   H ++ +  SS+
Sbjct: 366 VVPDQYTESRTSGDLVFYLDGAHSPESMEACAKWFSVAVKGDNQSGSSGHLVNGSAGSSH 425

Query: 422 KLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKAVFVPNTSLFN 481
                + N+       Q+LLFNCMSVRDP LLL  L   CA  GV+FKKA+FVPN S+++
Sbjct: 426 ---DKWSNETCE----QILLFNCMSVRDPNLLLPHLKNMCAKYGVNFKKALFVPNMSVYH 485

Query: 482 KVGTHG-LAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESEKPEVSGKSC 541
           KVGT   L E + +VDLSWQF +++VWE+++QSE+ G                E  G+S 
Sbjct: 486 KVGTAADLPENDPQVDLSWQFTLQKVWESLVQSERDG----------------EKDGESD 545

Query: 542 ENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 586
            NS VF+SLPMAIK LRD+V + + + R QVLVTGSLHLVGDVL+LI+
Sbjct: 546 GNSEVFTSLPMAIKCLRDTVHESSSATRFQVLVTGSLHLVGDVLRLIR 570

[Close]

BLAST of Csor.00g121400 vs. ExPASy Swiss-Prot
Match: Q8W035 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS3 PE=1 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 1.1e-157
Identity = 298/525 (56.76%), Postives = 364/525 (69.33%), Query Frame = 0


Query: 63  YEEALDVLSSLIVSRRR--CDKSGDRFELLFEYVKMLDLEESMSKMKVIHVAGTKGKGST 122
           Y+  LD LSSLI  R R   +    RF LLF Y+K+L+LE+++S+MK+IHVAGTKGKGST
Sbjct: 13  YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGTKGKGST 72

Query: 123 CTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWWCYNRLKEKTSED 182
           CTF ESILR  G  TGLFTSPHLIDVRERFRL+GI+IS+EKF+ YFW C+++LKEKTS +
Sbjct: 73  CTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLKEKTSNE 132

Query: 183 VPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYDHTEL 242
           VPMP  F FLALLAFKIF  EQVDV ILEVGLGG FDATNV+Q P+VCGI+SLGYDH E+
Sbjct: 133 VPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNVIQKPVVCGISSLGYDHMEI 192

Query: 243 LGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTVVPSLDPELLQGH 302
           LG TL  IA+EKAGIFK G PAFTV QPDEAM VL +KAS+L+V L VV  LD       
Sbjct: 193 LGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKLEVNLQVVEPLD----SSQ 252

Query: 303 KLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEGLTTASFEGRAQI 362
           +LGL+GEHQYLNA LA+AL ST+L   G  + +  +Q+  LPE FI GL+ A   GRA I
Sbjct: 253 RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMI 312

Query: 363 VPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDHQLDDTGTSSNKL 422
           VPD     E PE++V+YLDGAHSPESM  C+ WF   IK NQE                 
Sbjct: 313 VPDS----ELPEEIVYYLDGAHSPESMEACAIWFSKQIKQNQE----------------- 372

Query: 423 LQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKAVFVPNTSLFNKV 482
                    +K+S Q+LLFNCMSVRDP LLL  L   C + GV FK+AVFVPN S++N+V
Sbjct: 373 -------RNQKRSEQILLFNCMSVRDPSLLLPRLRSKCIDQGVDFKRAVFVPNVSVYNQV 432

Query: 483 GTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESEKPEVSGKSCENS 542
           G+        E  +SWQF ++R+WE++ + E   +S            K +  GK  E S
Sbjct: 433 GSSTNVGTRVE-SMSWQFGLQRIWESLARGEAKSNS------------KSDSKGKEEEKS 491

Query: 543 AVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 586
            VFSSLP+A+ WLRD+  +Q++ +R QVLVTGSLHLVGD+L+ IK
Sbjct: 493 FVFSSLPVAVDWLRDNA-RQSKQVRFQVLVTGSLHLVGDLLRFIK 491

[Close]

BLAST of Csor.00g121400 vs. ExPASy Swiss-Prot
Match: F4J2K2 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS2 PE=1 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 1.9e-144
Identity = 279/554 (50.36%), Postives = 370/554 (66.79%), Query Frame = 0


Query: 61  TAYEEALDVLSSLIVSRRRCDK-----SGDRFELLFEYVKMLDLEESMSKMKVIHVAGTK 120
           ++Y++A++ LS+LI  R R D+     + D+ E +  Y+K+LDLE+ + ++KVIHVAGTK
Sbjct: 81  SSYDDAMEALSTLISRRNRGDRTPTKGNRDKLEQVVTYLKILDLEDKIKELKVIHVAGTK 140

Query: 121 GKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWWCYNRLKE 180
           GKGSTC F+E+ILR CGF TG+FTSPHLIDVRERFR+DG+DISEEKFL YFW C+  LKE
Sbjct: 141 GKGSTCVFSEAILRNCGFRTGMFTSPHLIDVRERFRIDGLDISEEKFLQYFWECWKLLKE 200

Query: 181 KTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGY 240
           K  + + MP LF+FL +LAFKIF  E+VDVA++EVGLGG  D+TNV+Q P+VCGIASLG 
Sbjct: 201 KAVDGLTMPPLFQFLTVLAFKIFVCEKVDVAVIEVGLGGKLDSTNVIQKPVVCGIASLGM 260

Query: 241 DHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTVVPSLDPE 300
           DH ++LGNTL  IA  KAGIFK   PAFTV Q  EAM VL+  A+ L+V L VV  L+P+
Sbjct: 261 DHMDILGNTLADIAFHKAGIFKPQIPAFTVPQLSEAMDVLQKTANNLEVPLEVVAPLEPK 320

Query: 301 LLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQS--GSLPEHFIEGLTTAS 360
            L G  LGL G+HQ +NA LA++LS  WL RTG+    +  +S    +P  F  GL TA 
Sbjct: 321 KLDGVTLGLSGDHQLVNAGLAVSLSRCWLQRTGNWKKIFPNESKETEIPVAFCRGLATAR 380

Query: 361 FEGRAQIVPDRYND-------IESP-EDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGI 420
             GRAQ+V D  +D       +E+P  DL+FYLDGAHSPESM  C  WF +A++ ++   
Sbjct: 381 LHGRAQVVHDVVSDPQDSSDSMETPCGDLIFYLDGAHSPESMEACGRWFSSAVRGDK--- 440

Query: 421 LDHQLDDTGTSSNKLLQ-GYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVH 480
                    T+ N  ++ G    +  + S Q+LLFNCM VRDPQ+LL  L+ TCA++G H
Sbjct: 441 ------SLSTAVNGYMRHGEYGTDLNRVSKQILLFNCMEVRDPQVLLPKLVTTCASSGTH 500

Query: 481 FKKAVFVPNTSLFNKV--GTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGG-----DSQ 540
           F +A+FVP+ S +NKV  G   +       DL+WQF ++R+WE  IQ    G        
Sbjct: 501 FSRALFVPSMSTYNKVISGASAIPSDTRRKDLTWQFRLQRLWEKSIQGTDAGLDHTLKPD 560

Query: 541 GIGAMVEEE---SEKPEVSGKS---CENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVT 586
           GI A+   +    + P+  G +     +SAV  SLP+ I WLRD V ++N S++L+VLVT
Sbjct: 561 GITALPPHDFLCGDAPQCGGPAGTPVTSSAVMPSLPLTINWLRDCV-RRNPSLKLEVLVT 620

[Close]

BLAST of Csor.00g121400 vs. ExPASy Swiss-Prot
Match: A6H751 (Folylpolyglutamate synthase, mitochondrial OS=Bos taurus OX=9913 GN=FPGS PE=2 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 1.1e-80
Identity = 217/604 (35.93%), Postives = 303/604 (50.17%), Query Frame = 0


Query: 47  VTMEEGMGA---LKYSRTAYEEALDVLSSL--------IVSRRRCDKSGDRFELLFEYVK 106
           V +  G+ A   L+     Y++A+  L++L        +V R+R D    + E +  Y+ 
Sbjct: 23  VAVRRGLSAWPVLQEPDMEYQDAVRTLNTLQTNANYLEMVKRKRGDPQ-TQLEAMKLYLA 82

Query: 107 MLDLE-ESMSKMKVIHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDG 166
              L+ E + ++ +IH+ GTKGKGSTC FTE ILR  G  TG F+SPHL+ VRER R++G
Sbjct: 83  RSGLQVEDLDQLNIIHITGTKGKGSTCAFTERILRSYGLKTGFFSSPHLVQVRERIRING 142

Query: 167 IDISEEKFLAYFWWCYNRLKEKTSED--VPMPHLFRFLALLAFKIFADEQVDVAILEVGL 226
             IS E F  +FW  Y+RL+E   +   V MP  FRFL L+AF +F  E+VD+A+LEVG+
Sbjct: 143 QPISSELFTKHFWRLYHRLEETKDDSSCVSMPGYFRFLTLMAFHVFLQEKVDLAVLEVGI 202

Query: 227 GGTFDATNVVQAPIVCGIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAM 286
           GG +D TN+++ P+VCGI SLG DHT LLG+T+  IA +K GIFK G PAFTV QPDE +
Sbjct: 203 GGAYDCTNIIRKPVVCGITSLGIDHTGLLGDTMEKIAWQKGGIFKSGVPAFTVLQPDEPL 262

Query: 287 HVLRDKASQLDVQLTVVPSLDPELLQGH---KLGLEGEHQYLNARLAIALSSTWLHRTGH 346
            VLRD+A Q+   L + P L   L +G     LGLEGEHQ  NA LA+ ++  WL + G+
Sbjct: 263 AVLRDRAQQISCPLYLCPPLQ-ALEEGGPPLTLGLEGEHQRSNAALALQVARCWLQQKGY 322

Query: 347 SNLSYKEQS-----GSLP--------EHFIEGLTTASFEGRAQIVPDRYNDIESPEDLVF 406
            +    + S     G LP            +GL    + GR Q++            L +
Sbjct: 323 QDAGELKASRPSLPGQLPLAPVFQPTPRMQQGLRHTEWPGRTQLL--------RRGPLTW 382

Query: 407 YLDGAHSPESMVVCSSWFCAAIKHNQEGILDHQLDDTGTSSNKLLQGYDNDEARKKSVQV 466
           YLDGAH+  SM  C  WF  A+         H+ +   + S                V+V
Sbjct: 383 YLDGAHTTSSMQACVRWFRQAL---------HRCERPDSGS---------------EVRV 442

Query: 467 LLFNCMSVRDPQLLLQPLIKTCANNGVHFKKAVFVPNTSLFNKVGTHGLAEANGEVD--- 526
           LLFN    RD   LL+ L++ C      F  A+F PN  L     T    + N  V    
Sbjct: 443 LLFNSTGDRDSAALLK-LLQPC-----QFDYAIFCPN--LAEMASTDNADQQNFTVTLDQ 502

Query: 527 -----LSWQFHVRRVWENIIQ----SEKGGDSQGIGAMVEEESEKPEVSGKSCENSAVFS 586
                L+ Q H  R+ E  +     S  G +  G  +++      P        +S VFS
Sbjct: 503 VLLRCLAHQQHWSRLHEEQVSPDPWSTPGQEQDGPASLL----LAPHPPHTHSASSLVFS 562

[Close]

BLAST of Csor.00g121400 vs. ExPASy Swiss-Prot
Match: P48760 (Folylpolyglutamate synthase, mitochondrial OS=Mus musculus OX=10090 GN=Fpgs PE=1 SV=3)

HSP 1 Score: 300.4 bits (768), Expect = 4.3e-80
Identity = 205/580 (35.34%), Postives = 291/580 (50.17%), Query Frame = 0


Query: 63  YEEALDVLSSL--------IVSRRRCDKSGDRFELLFEYVKMLDLE-ESMSKMKVIHVAG 122
           Y++A+  L++L         V R+R D    + E +  Y+    L+ E ++++ +IHV G
Sbjct: 45  YQDAVRTLNTLQTNASYLEQVKRQRSDPQA-QLEAMEMYLARSGLQVEDLNRLNIIHVTG 104

Query: 123 TKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWWCYNRL 182
           TKGKGSTC FTE ILR  G  TG F+SPH++ VRER R++G  IS E F  +FW  YN+L
Sbjct: 105 TKGKGSTCAFTERILRNYGLKTGFFSSPHMVQVRERIRINGKPISPELFTKHFWCLYNQL 164

Query: 183 KE-KTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVCGIAS 242
           +E K    V MP  FRFL L+AF +F  E+VD+A++EVG+GG FD TN+++ P+VCG++S
Sbjct: 165 EEFKDDSHVSMPSYFRFLTLMAFHVFLQEKVDLAVVEVGIGGAFDCTNIIRKPVVCGVSS 224

Query: 243 LGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTVVPSL 302
           LG DHT LLG+T+  IA +K GIFK G PAFTV QP+  + VLRD+A Q+   L + P L
Sbjct: 225 LGIDHTSLLGDTVEKIAWQKGGIFKPGVPAFTVVQPEGPLAVLRDRAQQIGCPLYLCPPL 284

Query: 303 D--PELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQS-----GSLP--- 362
           +   E+     LGLEG HQ  NA LA+ L+  WL R  H ++   + S       LP   
Sbjct: 285 EALEEVGLPLSLGLEGAHQRSNAALALQLAHCWLERQDHQDIQELKVSRPSIRWQLPLAP 344

Query: 363 -----EHFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAA 422
                 H   GL    + GR QI+            L +YLDGAH+  S+  C  W+  +
Sbjct: 345 VFRPTPHMRRGLRDTVWPGRTQIL--------QRGPLTWYLDGAHTTSSVQACVHWYRQS 404

Query: 423 IKHNQEGILDHQLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKT 482
           ++ ++         D G+                  V +LLFN    RD   LL+ L++ 
Sbjct: 405 LERSKR-------TDGGS-----------------EVHILLFNSTGDRDSAALLK-LLQP 464

Query: 483 CANNGVHFKKAVFVPNTSLFNKVGTHGLAEANGEVD----------LSWQFHVRRVWENI 542
           C      F  AVF PN +  + +G          +D            W     +   + 
Sbjct: 465 C-----QFDYAVFCPNVTEVSSIGNADQQNFTVTLDQVLLRCLQHQQHWNGLAEKQASSN 524

Query: 543 IQSEKGGDSQGIGAMVEEESEKPEVSGKSCENSAVFSSLPMAIKWL---RDSVLQQ---- 585
           + S  G D  G G+++      P     +  +S VFS +  A+ W+   RD + Q     
Sbjct: 525 LWSSCGPDPAGPGSLL----LAPHPPQPTRTSSLVFSCISHALLWISQGRDPIFQPQSLP 581

[Close]

BLAST of Csor.00g121400 vs. NCBI nr
Match: KAG6602447.1 (Folylpolyglutamate synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1172 bits (3031), Expect = 0.0
Identity = 585/585 (100.00%), Postives = 585/585 (100.00%), Query Frame = 0


Query: 1   MPLSFSSRPLPVFGTRPRLLRRSRKEEIGEKTRLNPQDIGEAGGHLVTMEEGMGALKYSR 60
           MPLSFSSRPLPVFGTRPRLLRRSRKEEIGEKTRLNPQDIGEAGGHLVTMEEGMGALKYSR
Sbjct: 1   MPLSFSSRPLPVFGTRPRLLRRSRKEEIGEKTRLNPQDIGEAGGHLVTMEEGMGALKYSR 60

Query: 61  TAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKVIHVAGTKGKGST 120
           TAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKVIHVAGTKGKGST
Sbjct: 61  TAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKVIHVAGTKGKGST 120

Query: 121 CTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWWCYNRLKEKTSED 180
           CTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWWCYNRLKEKTSED
Sbjct: 121 CTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWWCYNRLKEKTSED 180

Query: 181 VPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYDHTEL 240
           VPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYDHTEL
Sbjct: 181 VPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYDHTEL 240

Query: 241 LGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTVVPSLDPELLQGH 300
           LGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTVVPSLDPELLQGH
Sbjct: 241 LGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTVVPSLDPELLQGH 300

Query: 301 KLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEGLTTASFEGRAQI 360
           KLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEGLTTASFEGRAQI
Sbjct: 301 KLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEGLTTASFEGRAQI 360

Query: 361 VPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDHQLDDTGTSSNKL 420
           VPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDHQLDDTGTSSNKL
Sbjct: 361 VPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDHQLDDTGTSSNKL 420

Query: 421 LQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKAVFVPNTSLFNKV 480
           LQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKAVFVPNTSLFNKV
Sbjct: 421 LQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKAVFVPNTSLFNKV 480

Query: 481 GTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESEKPEVSGKSCENS 540
           GTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESEKPEVSGKSCENS
Sbjct: 481 GTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESEKPEVSGKSCENS 540

Query: 541 AVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 585
           AVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 541 AVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 585

[Close]

BLAST of Csor.00g121400 vs. NCBI nr
Match: KAG7033130.1 (Folylpolyglutamate synthase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1076 bits (2782), Expect = 0.0
Identity = 536/537 (99.81%), Postives = 536/537 (99.81%), Query Frame = 0


Query: 49  MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 108
           MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 60

Query: 109 IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWW 168
           IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDG DISEEKFLAYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGFDISEEKFLAYFWW 120

Query: 169 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 228
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 229 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 288
           GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 240

Query: 289 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEG 348
           VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEG
Sbjct: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEG 300

Query: 349 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 408
           LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH
Sbjct: 301 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 360

Query: 409 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 468
           QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA
Sbjct: 361 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 420

Query: 469 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 528
           VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE
Sbjct: 421 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 480

Query: 529 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 585
           KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537

[Close]

BLAST of Csor.00g121400 vs. NCBI nr
Match: XP_022961882.1 (folylpolyglutamate synthase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1073 bits (2774), Expect = 0.0
Identity = 535/537 (99.63%), Postives = 535/537 (99.63%), Query Frame = 0


Query: 49  MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 108
           MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 60

Query: 109 IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWW 168
           IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWW 120

Query: 169 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 228
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 229 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 288
           GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 240

Query: 289 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEG 348
           VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPE FIEG
Sbjct: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEQFIEG 300

Query: 349 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 408
           LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH
Sbjct: 301 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 360

Query: 409 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 468
           QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTC NNGVHFKKA
Sbjct: 361 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCTNNGVHFKKA 420

Query: 469 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 528
           VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE
Sbjct: 421 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 480

Query: 529 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 585
           KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537

[Close]

BLAST of Csor.00g121400 vs. NCBI nr
Match: XP_023533507.1 (folylpolyglutamate synthase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1065 bits (2755), Expect = 0.0
Identity = 529/537 (98.51%), Postives = 533/537 (99.26%), Query Frame = 0


Query: 49  MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 108
           MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFEL+FEYVKMLDLEESMSKMKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 60

Query: 109 IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWW 168
           IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLID+RERFRLDGIDISEEKFLAYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 120

Query: 169 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 228
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIF+DEQVDVAILEVGLGGTFDATNVVQAPIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 229 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 288
           GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 240

Query: 289 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEG 348
           VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPE FIEG
Sbjct: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEQFIEG 300

Query: 349 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 408
           LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH
Sbjct: 301 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 360

Query: 409 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 468
           QLDD GTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA
Sbjct: 361 QLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 420

Query: 469 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 528
           VFVPNTS+FNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIG MVEEESE
Sbjct: 421 VFVPNTSVFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGTMVEEESE 480

Query: 529 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 585
           KPEVSGK CENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVSGKCCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537

[Close]

BLAST of Csor.00g121400 vs. NCBI nr
Match: XP_022961890.1 (folylpolyglutamate synthase-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1056 bits (2731), Expect = 0.0
Identity = 530/537 (98.70%), Postives = 530/537 (98.70%), Query Frame = 0


Query: 49  MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 108
           MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 60

Query: 109 IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWW 168
           IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWW 120

Query: 169 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 228
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 229 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 288
           GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 240

Query: 289 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEG 348
           VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPE FIEG
Sbjct: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEQFIEG 300

Query: 349 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 408
           LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH
Sbjct: 301 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 360

Query: 409 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 468
           QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTC NNGVHFKKA
Sbjct: 361 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCTNNGVHFKKA 420

Query: 469 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 528
           VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKG     IGAMVEEESE
Sbjct: 421 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKG-----IGAMVEEESE 480

Query: 529 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 585
           KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 532

[Close]

BLAST of Csor.00g121400 vs. ExPASy TrEMBL
Match: A0A6J1HBB1 (Folylpolyglutamate synthase OS=Cucurbita moschata OX=3662 GN=LOC111462519 PE=3 SV=1)

HSP 1 Score: 1073 bits (2774), Expect = 0.0
Identity = 535/537 (99.63%), Postives = 535/537 (99.63%), Query Frame = 0


Query: 49  MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 108
           MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 60

Query: 109 IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWW 168
           IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWW 120

Query: 169 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 228
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 229 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 288
           GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 240

Query: 289 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEG 348
           VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPE FIEG
Sbjct: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEQFIEG 300

Query: 349 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 408
           LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH
Sbjct: 301 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 360

Query: 409 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 468
           QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTC NNGVHFKKA
Sbjct: 361 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCTNNGVHFKKA 420

Query: 469 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 528
           VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE
Sbjct: 421 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 480

Query: 529 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 585
           KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537

[Close]

BLAST of Csor.00g121400 vs. ExPASy TrEMBL
Match: A0A6J1HDI1 (Folylpolyglutamate synthase OS=Cucurbita moschata OX=3662 GN=LOC111462519 PE=3 SV=1)

HSP 1 Score: 1056 bits (2731), Expect = 0.0
Identity = 530/537 (98.70%), Postives = 530/537 (98.70%), Query Frame = 0


Query: 49  MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 108
           MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 60

Query: 109 IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWW 168
           IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWW 120

Query: 169 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 228
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 229 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 288
           GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 240

Query: 289 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEG 348
           VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPE FIEG
Sbjct: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEQFIEG 300

Query: 349 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 408
           LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH
Sbjct: 301 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 360

Query: 409 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 468
           QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTC NNGVHFKKA
Sbjct: 361 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCTNNGVHFKKA 420

Query: 469 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 528
           VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKG     IGAMVEEESE
Sbjct: 421 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKG-----IGAMVEEESE 480

Query: 529 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 585
           KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 532

[Close]

BLAST of Csor.00g121400 vs. ExPASy TrEMBL
Match: A0A6J1JQH1 (Folylpolyglutamate synthase OS=Cucurbita maxima OX=3661 GN=LOC111487415 PE=3 SV=1)

HSP 1 Score: 1053 bits (2723), Expect = 0.0
Identity = 524/537 (97.58%), Postives = 531/537 (98.88%), Query Frame = 0


Query: 49  MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 108
           MEEGMGALKYSRTAYEEALDVL SLIV+RRRCDKSGDRFEL+FEYVKMLDLEESMSKMKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLFSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 60

Query: 109 IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWW 168
           IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLID+RERFRLDGIDISEEKFLAYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 120

Query: 169 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 228
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIF+DEQVDVAILEVGLGGTFDATNVVQAPIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 229 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 288
           GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTV QPDEAMHVLRDKASQLDVQLTV
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240

Query: 289 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEG 348
           VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTG SNLSYKEQSGSLPE FIEG
Sbjct: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGRSNLSYKEQSGSLPEQFIEG 300

Query: 349 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 408
           LTTA FEGRAQIVPDRYNDIE+PEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGIL+H
Sbjct: 301 LTTARFEGRAQIVPDRYNDIETPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILNH 360

Query: 409 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 468
           QLDD GTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA
Sbjct: 361 QLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 420

Query: 469 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 528
           +FVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE
Sbjct: 421 LFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 480

Query: 529 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 585
           KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537

[Close]

BLAST of Csor.00g121400 vs. ExPASy TrEMBL
Match: A0A6J1JTP4 (Folylpolyglutamate synthase OS=Cucurbita maxima OX=3661 GN=LOC111487415 PE=3 SV=1)

HSP 1 Score: 1038 bits (2684), Expect = 0.0
Identity = 524/565 (92.74%), Postives = 531/565 (93.98%), Query Frame = 0


Query: 49  MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 108
           MEEGMGALKYSRTAYEEALDVL SLIV+RRRCDKSGDRFEL+FEYVKMLDLEESMSKMKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLFSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 60

Query: 109 IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWW 168
           IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLID+RERFRLDGIDISEEKFLAYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 120

Query: 169 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 228
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIF+DEQVDVAILEVGLGGTFDATNVVQAPIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 229 GIASLGYDHTELLGNTLGAIASEKAGIFK----------------------------RGA 288
           GIASLGYDHTELLGNTLGAIASEKAGIFK                            RGA
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKVCLKKLSLKHERLGIISSFCFHPFTLVQRGA 240

Query: 289 PAFTVCQPDEAMHVLRDKASQLDVQLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALS 348
           PAFTV QPDEAMHVLRDKASQLDVQLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALS
Sbjct: 241 PAFTVFQPDEAMHVLRDKASQLDVQLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALS 300

Query: 349 STWLHRTGHSNLSYKEQSGSLPEHFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDG 408
           STWLHRTG SNLSYKEQSGSLPE FIEGLTTA FEGRAQIVPDRYNDIE+PEDLVFYLDG
Sbjct: 301 STWLHRTGRSNLSYKEQSGSLPEQFIEGLTTARFEGRAQIVPDRYNDIETPEDLVFYLDG 360

Query: 409 AHSPESMVVCSSWFCAAIKHNQEGILDHQLDDTGTSSNKLLQGYDNDEARKKSVQVLLFN 468
           AHSPESMVVCSSWFCAAIKHNQEGIL+HQLDD GTSSNKLLQGYDNDEARKKSVQVLLFN
Sbjct: 361 AHSPESMVVCSSWFCAAIKHNQEGILNHQLDDNGTSSNKLLQGYDNDEARKKSVQVLLFN 420

Query: 469 CMSVRDPQLLLQPLIKTCANNGVHFKKAVFVPNTSLFNKVGTHGLAEANGEVDLSWQFHV 528
           CMSVRDPQLLLQPLIKTCANNGVHFKKA+FVPNTSLFNKVGTHGLAEANGEVDLSWQFHV
Sbjct: 421 CMSVRDPQLLLQPLIKTCANNGVHFKKALFVPNTSLFNKVGTHGLAEANGEVDLSWQFHV 480

Query: 529 RRVWENIIQSEKGGDSQGIGAMVEEESEKPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQ 585
           RRVWENIIQSEKGGDSQGIGAMVEEESEKPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQ
Sbjct: 481 RRVWENIIQSEKGGDSQGIGAMVEEESEKPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQ 540

[Close]

BLAST of Csor.00g121400 vs. ExPASy TrEMBL
Match: A0A6J1JNC2 (Folylpolyglutamate synthase OS=Cucurbita maxima OX=3661 GN=LOC111487415 PE=3 SV=1)

HSP 1 Score: 1036 bits (2680), Expect = 0.0
Identity = 519/537 (96.65%), Postives = 526/537 (97.95%), Query Frame = 0


Query: 49  MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 108
           MEEGMGALKYSRTAYEEALDVL SLIV+RRRCDKSGDRFEL+FEYVKMLDLEESMSKMKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLFSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 60

Query: 109 IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWW 168
           IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLID+RERFRLDGIDISEEKFLAYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 120

Query: 169 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 228
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIF+DEQVDVAILEVGLGGTFDATNVVQAPIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 229 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 288
           GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTV QPDEAMHVLRDKASQLDVQLTV
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240

Query: 289 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEG 348
           VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTG SNLSYKEQSGSLPE FIEG
Sbjct: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGRSNLSYKEQSGSLPEQFIEG 300

Query: 349 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 408
           LTTA FEGRAQIVPDRYNDIE+PEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGIL+H
Sbjct: 301 LTTARFEGRAQIVPDRYNDIETPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILNH 360

Query: 409 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 468
           QLDD GTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA
Sbjct: 361 QLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 420

Query: 469 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 528
           +FVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKG     IGAMVEEESE
Sbjct: 421 LFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKG-----IGAMVEEESE 480

Query: 529 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 585
           KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 532

[Close]

BLAST of Csor.00g121400 vs. TAIR 10
Match: AT5G05980.1 (DHFS-FPGS homolog B )

HSP 1 Score: 636.7 bits (1641), Expect = 1.8e-182
Identity = 330/528 (62.50%), Postives = 395/528 (74.81%), Query Frame = 0


Query: 62  AYEEALDVLSSLIVSRRRCDKS--GDRFELLFEYVKMLDLEESMSKMKVIHVAGTKGKGS 121
           +YEEAL  LSSLI  R R DKS  GDRFEL+F+Y+K+LDLEE + KM VIHVAGTKGKGS
Sbjct: 66  SYEEALAALSSLITKRSRADKSNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKGKGS 125

Query: 122 TCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWWCYNRLKEKTSE 181
           TCTFTESI+R  GF TGLFTSPHLIDVRERFRLDG+DISEEKFL YFWWCYNRLKE+T+E
Sbjct: 126 TCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKERTNE 185

Query: 182 DVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYDHTE 241
           ++PMP  FRFLALLAFKIFA E+VD AILEVGLGG FDATN VQ P+VCGI+SLGYDH E
Sbjct: 186 EIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYDHME 245

Query: 242 LLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTVVPSLDPELLQG 301
           +LG+TLG IA EKAGIFK G PAFTV QPDEAM VL +KAS+ +V L VV  L   LL G
Sbjct: 246 ILGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASETEVNLEVVQPLTARLLSG 305

Query: 302 HKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEGLTTASFEGRAQ 361
            KLGL+GEHQY+NA LA++L+S WL + G   +  + Q   LPE FI+GL TAS +GRAQ
Sbjct: 306 QKLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVPSRTQMSILPEKFIKGLATASLQGRAQ 365

Query: 362 IVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIK-HNQEGILDHQLDDTGTSSN 421
           +VPD+Y +  +  DLVFYLDGAHSPESM  C+ WF  A+K  NQ G   H ++ +  SS+
Sbjct: 366 VVPDQYTESRTSGDLVFYLDGAHSPESMEACAKWFSVAVKGDNQSGSSGHLVNGSAGSSH 425

Query: 422 KLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKAVFVPNTSLFN 481
                + N+       Q+LLFNCMSVRDP LLL  L   CA  GV+FKKA+FVPN S+++
Sbjct: 426 ---DKWSNETCE----QILLFNCMSVRDPNLLLPHLKNMCAKYGVNFKKALFVPNMSVYH 485

Query: 482 KVGTHG-LAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESEKPEVSGKSC 541
           KVGT   L E + +VDLSWQF +++VWE+++QSE+ G                E  G+S 
Sbjct: 486 KVGTAADLPENDPQVDLSWQFTLQKVWESLVQSERDG----------------EKDGESD 545

Query: 542 ENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 586
            NS VF+SLPMAIK LRD+V + + + R QVLVTGSLHLVGDVL+LI+
Sbjct: 546 GNSEVFTSLPMAIKCLRDTVHESSSATRFQVLVTGSLHLVGDVLRLIR 570

[Close]

BLAST of Csor.00g121400 vs. TAIR 10
Match: AT5G05980.2 (DHFS-FPGS homolog B )

HSP 1 Score: 636.7 bits (1641), Expect = 1.8e-182
Identity = 330/528 (62.50%), Postives = 395/528 (74.81%), Query Frame = 0


Query: 62  AYEEALDVLSSLIVSRRRCDKS--GDRFELLFEYVKMLDLEESMSKMKVIHVAGTKGKGS 121
           +YEEAL  LSSLI  R R DKS  GDRFEL+F+Y+K+LDLEE + KM VIHVAGTKGKGS
Sbjct: 8   SYEEALAALSSLITKRSRADKSNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKGKGS 67

Query: 122 TCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWWCYNRLKEKTSE 181
           TCTFTESI+R  GF TGLFTSPHLIDVRERFRLDG+DISEEKFL YFWWCYNRLKE+T+E
Sbjct: 68  TCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKERTNE 127

Query: 182 DVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYDHTE 241
           ++PMP  FRFLALLAFKIFA E+VD AILEVGLGG FDATN VQ P+VCGI+SLGYDH E
Sbjct: 128 EIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYDHME 187

Query: 242 LLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTVVPSLDPELLQG 301
           +LG+TLG IA EKAGIFK G PAFTV QPDEAM VL +KAS+ +V L VV  L   LL G
Sbjct: 188 ILGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASETEVNLEVVQPLTARLLSG 247

Query: 302 HKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEGLTTASFEGRAQ 361
            KLGL+GEHQY+NA LA++L+S WL + G   +  + Q   LPE FI+GL TAS +GRAQ
Sbjct: 248 QKLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVPSRTQMSILPEKFIKGLATASLQGRAQ 307

Query: 362 IVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIK-HNQEGILDHQLDDTGTSSN 421
           +VPD+Y +  +  DLVFYLDGAHSPESM  C+ WF  A+K  NQ G   H ++ +  SS+
Sbjct: 308 VVPDQYTESRTSGDLVFYLDGAHSPESMEACAKWFSVAVKGDNQSGSSGHLVNGSAGSSH 367

Query: 422 KLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKAVFVPNTSLFN 481
                + N+       Q+LLFNCMSVRDP LLL  L   CA  GV+FKKA+FVPN S+++
Sbjct: 368 ---DKWSNETCE----QILLFNCMSVRDPNLLLPHLKNMCAKYGVNFKKALFVPNMSVYH 427

Query: 482 KVGTHG-LAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESEKPEVSGKSC 541
           KVGT   L E + +VDLSWQF +++VWE+++QSE+ G                E  G+S 
Sbjct: 428 KVGTAADLPENDPQVDLSWQFTLQKVWESLVQSERDG----------------EKDGESD 487

Query: 542 ENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 586
            NS VF+SLPMAIK LRD+V + + + R QVLVTGSLHLVGDVL+LI+
Sbjct: 488 GNSEVFTSLPMAIKCLRDTVHESSSATRFQVLVTGSLHLVGDVLRLIR 512

[Close]

BLAST of Csor.00g121400 vs. TAIR 10
Match: AT3G55630.3 (DHFS-FPGS homolog D )

HSP 1 Score: 558.1 bits (1437), Expect = 8.1e-159
Identity = 298/525 (56.76%), Postives = 364/525 (69.33%), Query Frame = 0


Query: 63  YEEALDVLSSLIVSRRR--CDKSGDRFELLFEYVKMLDLEESMSKMKVIHVAGTKGKGST 122
           Y+  LD LSSLI  R R   +    RF LLF Y+K+L+LE+++S+MK+IHVAGTKGKGST
Sbjct: 13  YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGTKGKGST 72

Query: 123 CTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWWCYNRLKEKTSED 182
           CTF ESILR  G  TGLFTSPHLIDVRERFRL+GI+IS+EKF+ YFW C+++LKEKTS +
Sbjct: 73  CTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLKEKTSNE 132

Query: 183 VPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYDHTEL 242
           VPMP  F FLALLAFKIF  EQVDV ILEVGLGG FDATNV+Q P+VCGI+SLGYDH E+
Sbjct: 133 VPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNVIQKPVVCGISSLGYDHMEI 192

Query: 243 LGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTVVPSLDPELLQGH 302
           LG TL  IA+EKAGIFK G PAFTV QPDEAM VL +KAS+L+V L VV  LD       
Sbjct: 193 LGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKLEVNLQVVEPLD----SSQ 252

Query: 303 KLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEGLTTASFEGRAQI 362
           +LGL+GEHQYLNA LA+AL ST+L   G  + +  +Q+  LPE FI GL+ A   GRA I
Sbjct: 253 RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMI 312

Query: 363 VPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDHQLDDTGTSSNKL 422
           VPD     E PE++V+YLDGAHSPESM  C+ WF   IK NQE                 
Sbjct: 313 VPDS----ELPEEIVYYLDGAHSPESMEACAIWFSKQIKQNQE----------------- 372

Query: 423 LQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKAVFVPNTSLFNKV 482
                    +K+S Q+LLFNCMSVRDP LLL  L   C + GV FK+AVFVPN S++N+V
Sbjct: 373 -------RNQKRSEQILLFNCMSVRDPSLLLPRLRSKCIDQGVDFKRAVFVPNVSVYNQV 432

Query: 483 GTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESEKPEVSGKSCENS 542
           G+        E  +SWQF ++R+WE++ + E   +S            K +  GK  E S
Sbjct: 433 GSSTNVGTRVE-SMSWQFGLQRIWESLARGEAKSNS------------KSDSKGKEEEKS 491

Query: 543 AVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 586
            VFSSLP+A+ WLRD+  +Q++ +R QVLVTGSLHLVGD+L+ IK
Sbjct: 493 FVFSSLPVAVDWLRDNA-RQSKQVRFQVLVTGSLHLVGDLLRFIK 491

[Close]

BLAST of Csor.00g121400 vs. TAIR 10
Match: AT3G55630.2 (DHFS-FPGS homolog D )

HSP 1 Score: 552.4 bits (1422), Expect = 4.5e-157
Identity = 297/525 (56.57%), Postives = 363/525 (69.14%), Query Frame = 0


Query: 63  YEEALDVLSSLIVSRRR--CDKSGDRFELLFEYVKMLDLEESMSKMKVIHVAGTKGKGST 122
           Y+  LD LSSLI  R R   +    RF LLF Y+K+L+LE+++S+MK+IHVAGTKGKGST
Sbjct: 13  YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGTKGKGST 72

Query: 123 CTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWWCYNRLKEKTSED 182
           CTF ESILR  G  TGLFTSPHLIDVRERFRL+GI+IS+EKF+ YFW C+++LKEKTS +
Sbjct: 73  CTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLKEKTSNE 132

Query: 183 VPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYDHTEL 242
           VPMP  F FLALLAFKIF  EQVDV ILEVGLGG FDATN +Q P+VCGI+SLGYDH E+
Sbjct: 133 VPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATN-IQKPVVCGISSLGYDHMEI 192

Query: 243 LGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTVVPSLDPELLQGH 302
           LG TL  IA+EKAGIFK G PAFTV QPDEAM VL +KAS+L+V L VV  LD       
Sbjct: 193 LGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKLEVNLQVVEPLD----SSQ 252

Query: 303 KLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEGLTTASFEGRAQI 362
           +LGL+GEHQYLNA LA+AL ST+L   G  + +  +Q+  LPE FI GL+ A   GRA I
Sbjct: 253 RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMI 312

Query: 363 VPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDHQLDDTGTSSNKL 422
           VPD     E PE++V+YLDGAHSPESM  C+ WF   IK NQE                 
Sbjct: 313 VPDS----ELPEEIVYYLDGAHSPESMEACAIWFSKQIKQNQE----------------- 372

Query: 423 LQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKAVFVPNTSLFNKV 482
                    +K+S Q+LLFNCMSVRDP LLL  L   C + GV FK+AVFVPN S++N+V
Sbjct: 373 -------RNQKRSEQILLFNCMSVRDPSLLLPRLRSKCIDQGVDFKRAVFVPNVSVYNQV 432

Query: 483 GTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESEKPEVSGKSCENS 542
           G+        E  +SWQF ++R+WE++ + E   +S            K +  GK  E S
Sbjct: 433 GSSTNVGTRVE-SMSWQFGLQRIWESLARGEAKSNS------------KSDSKGKEEEKS 490

Query: 543 AVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 586
            VFSSLP+A+ WLRD+  +Q++ +R QVLVTGSLHLVGD+L+ IK
Sbjct: 493 FVFSSLPVAVDWLRDNA-RQSKQVRFQVLVTGSLHLVGDLLRFIK 490

[Close]

BLAST of Csor.00g121400 vs. TAIR 10
Match: AT3G55630.1 (DHFS-FPGS homolog D )

HSP 1 Score: 515.0 bits (1325), Expect = 7.9e-146
Identity = 281/525 (53.52%), Postives = 347/525 (66.10%), Query Frame = 0


Query: 63  YEEALDVLSSLIVSRRR--CDKSGDRFELLFEYVKMLDLEESMSKMKVIHVAGTKGKGST 122
           Y+  LD LSSLI  R R   +    RF LLF Y+K+L+LE+++S+MK+IHVAGTKGKGST
Sbjct: 13  YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGTKGKGST 72

Query: 123 CTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWWCYNRLKEKTSED 182
           CTF ESILR  G  TGLFTSPHLIDVRERFRL+GI+IS+EKF+ YFW C+++LKEKTS  
Sbjct: 73  CTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLKEKTS-- 132

Query: 183 VPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYDHTEL 242
                                +VDV ILEVGLGG FDATNV+Q P+VCGI+SLGYDH E+
Sbjct: 133 --------------------NEVDVVILEVGLGGRFDATNVIQKPVVCGISSLGYDHMEI 192

Query: 243 LGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTVVPSLDPELLQGH 302
           LG TL  IA+EKAGIFK G PAFTV QPDEAM VL +KAS+L+V L VV  LD       
Sbjct: 193 LGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKLEVNLQVVEPLD----SSQ 252

Query: 303 KLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEGLTTASFEGRAQI 362
           +LGL+GEHQYLNA LA+AL ST+L   G  + +  +Q+  LPE FI GL+ A   GRA I
Sbjct: 253 RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMI 312

Query: 363 VPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDHQLDDTGTSSNKL 422
           VPD     E PE++V+YLDGAHSPESM  C+ WF   IK NQE                 
Sbjct: 313 VPDS----ELPEEIVYYLDGAHSPESMEACAIWFSKQIKQNQE----------------- 372

Query: 423 LQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKAVFVPNTSLFNKV 482
                    +K+S Q+LLFNCMSVRDP LLL  L   C + GV FK+AVFVPN S++N+V
Sbjct: 373 -------RNQKRSEQILLFNCMSVRDPSLLLPRLRSKCIDQGVDFKRAVFVPNVSVYNQV 432

Query: 483 GTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESEKPEVSGKSCENS 542
           G+        E  +SWQF ++R+WE++ + E   +S            K +  GK  E S
Sbjct: 433 GSSTNVGTRVE-SMSWQFGLQRIWESLARGEAKSNS------------KSDSKGKEEEKS 469

Query: 543 AVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 586
            VFSSLP+A+ WLRD+  +Q++ +R QVLVTGSLHLVGD+L+ IK
Sbjct: 493 FVFSSLPVAVDWLRDNA-RQSKQVRFQVLVTGSLHLVGDLLRFIK 469

The following BLAST results are available for this feature:

BLAST of Csor.00g121400 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4K2A1	2.5e-181	62.50	Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS1 PE=1 SV=1	[more]
Q8W035	1.1e-157	56.76	Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS3 PE=1 SV=1	[more]
F4J2K2	1.9e-144	50.36	Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS2 PE=1 SV=1	[more]
A6H751	1.1e-80	35.93	Folylpolyglutamate synthase, mitochondrial OS=Bos taurus OX=9913 GN=FPGS PE=2 SV...	[more]
P48760	4.3e-80	35.34	Folylpolyglutamate synthase, mitochondrial OS=Mus musculus OX=10090 GN=Fpgs PE=1...	[more]

BLAST of Csor.00g121400 vs. NCBI nr


Match Name	Identity	Description
KAG6602447.1	100.00	Folylpolyglutamate synthase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7033130.1	99.81	Folylpolyglutamate synthase [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022961882.1	99.63	folylpolyglutamate synthase-like isoform X1 [Cucurbita moschata]	[more]
XP_023533507.1	98.51	folylpolyglutamate synthase-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022961890.1	98.70	folylpolyglutamate synthase-like isoform X2 [Cucurbita moschata]	[more]

BLAST of Csor.00g121400 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1HBB1	99.63	Folylpolyglutamate synthase OS=Cucurbita moschata OX=3662 GN=LOC111462519 PE=3 S...	[more]
A0A6J1HDI1	98.70	Folylpolyglutamate synthase OS=Cucurbita moschata OX=3662 GN=LOC111462519 PE=3 S...	[more]
A0A6J1JQH1	97.58	Folylpolyglutamate synthase OS=Cucurbita maxima OX=3661 GN=LOC111487415 PE=3 SV=...	[more]
A0A6J1JTP4	92.74	Folylpolyglutamate synthase OS=Cucurbita maxima OX=3661 GN=LOC111487415 PE=3 SV=...	[more]
A0A6J1JNC2	96.65	Folylpolyglutamate synthase OS=Cucurbita maxima OX=3661 GN=LOC111487415 PE=3 SV=...	[more]

BLAST of Csor.00g121400 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G05980.1	1.8e-182	62.50	DHFS-FPGS homolog B	[more]
AT5G05980.2	1.8e-182	62.50	DHFS-FPGS homolog B	[more]
AT3G55630.3	8.1e-159	56.76	DHFS-FPGS homolog D	[more]
AT3G55630.2	4.5e-157	56.57	DHFS-FPGS homolog D	[more]
AT3G55630.1	7.9e-146	53.52	DHFS-FPGS homolog D	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001645	Folylpolyglutamate synthetase	TIGRFAM	TIGR01499	TIGR01499	coord: 100..400 e-value: 5.4E-87 score: 290.7
IPR001645	Folylpolyglutamate synthetase	PANTHER	PTHR11136	FOLYLPOLYGLUTAMATE SYNTHASE-RELATED	coord: 58..585
IPR023600	Folylpolyglutamate synthase, eukaryota	PIRSF	PIRSF038895	FPGS	coord: 48..585 e-value: 7.8E-150 score: 496.9
IPR013221	Mur ligase, central	PFAM	PF08245	Mur_ligase_M	coord: 111..285 e-value: 1.7E-4 score: 21.6
IPR036565	Mur-like, catalytic domain superfamily	GENE3D	3.40.1190.10		coord: 53..337 e-value: 6.2E-89 score: 299.9
IPR036565	Mur-like, catalytic domain superfamily	SUPERFAMILY	53623	MurD-like peptide ligases, catalytic domain	coord: 79..325
IPR036615	Mur ligase, C-terminal domain superfamily	GENE3D	3.90.190.20		coord: 356..585 e-value: 1.3E-9 score: 40.4
IPR036615	Mur ligase, C-terminal domain superfamily	SUPERFAMILY	53244	MurD-like peptide ligases, peptide-binding domain	coord: 356..584
None	No IPR available	PANTHER	PTHR11136:SF10	FOLYLPOLYGLUTAMATE SYNTHASE	coord: 58..585
IPR018109	Folylpolyglutamate synthetase, conserved site	PROSITE	PS01012	FOLYLPOLYGLU_SYNT_2	coord: 207..222
IPR018109	Folylpolyglutamate synthetase, conserved site	PROSITE	PS01011	FOLYLPOLYGLU_SYNT_1	coord: 109..132

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g121400.m01	Csor.00g121400.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006730	one-carbon metabolic process
biological_process	GO:0046901	tetrahydrofolylpolyglutamate biosynthetic process
biological_process	GO:0009058	biosynthetic process
biological_process	GO:0009396	folic acid-containing compound biosynthetic process
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004326	tetrahydrofolylpolyglutamate synthase activity
molecular_function	GO:0016874	ligase activity