Csor.00g121250 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g121250
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionmetalloendoproteinase 2-MMP-like
LocationCsor_Chr04: 19736131 .. 19737123 (-)
RNA-Seq ExpressionCsor.00g121250
SyntenyCsor.00g121250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSsinglepolypeptidestart_codonstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCAAACCCCTTCAACTCCTCGCTTTCACCCTTCTCCTCCCTTCCCTCTTCCCTCGTTTCGACATCGTGTCGGTCCAATGCCACTCAGAATTGGAAGCTGTGGGCTCATCTCCGATCATTTTGAGTCCTAATTCCCACGCCCCGAACTCGTCCTCCTTGTTGTTTCTCAACAATCTCCAAGGATGTCGTTTGGGTGACACAGCCCAAGGTATTCATCGGATAAAAAAGTACCTTCAACGCTTTGGTTACATTACCAATGTCCAAAAACAATCCAACGCCATGGATCATGATGACACCTTTGATAATGCCTTGGAATCCGCCCTTAAAACGTACCAAAAAAACTTCAATCTTGTTGCCTCTGGAATTTTGGACGCCAACACATTAGCTCAAATGGGGAAGCCACGATGTGGGGTTGAAGATGTTATAAATGGCACAACTTGGATGAGATCGGGAAGGGAAAGGAAGCCAAAAATGCATGCTCATTTTCACTTAGTGTCTCACTTTGCTTTCTTTGAAGGAAACCTCAAATGGCCGCCTTCAAAGTTCCACTTGACCTATGCATTTCTTCCAGGGTATCCCTTTGAGGCAATGATGGCAGTGTCGAGGGCTTTTTCCAAGTGGGCTTTCAATACCCACTTCACATTTTCACGTGTTTTTTACCATACAAAAGCTGATATCAAAATAAGCTTTGAAATAGGAGACCATGGAGATGATGTGCCGTTTGATGGTGCGGGGGGGATTCTGGCGCATGCTTATGCGCCCACGGACGGCAGGCTTCACTTTGATGGCGATGAGGCTTGGTCTGTGGGAGCTGTTGATGGTTATTTTGATGTGGAGACTATTGCGTTGCATGAGATTGGACACGTTCTAGGGCTTCAACATAGCTCCATTGAAAACGCTATTATGTTCCCAAGCATATCGGAGGGAGTTACTAAGGGTTTGCATGGGGATGACATAGCTGGGATTAGGGCTCTATATAAAGTTTGA

mRNA sequence

ATGGCTTCCAAACCCCTTCAACTCCTCGCTTTCACCCTTCTCCTCCCTTCCCTCTTCCCTCGTTTCGACATCGTGTCGGTCCAATGCCACTCAGAATTGGAAGCTGTGGGCTCATCTCCGATCATTTTGAGTCCTAATTCCCACGCCCCGAACTCGTCCTCCTTGTTGTTTCTCAACAATCTCCAAGGATGTCGTTTGGGTGACACAGCCCAAGGTATTCATCGGATAAAAAAGTACCTTCAACGCTTTGGTTACATTACCAATGTCCAAAAACAATCCAACGCCATGGATCATGATGACACCTTTGATAATGCCTTGGAATCCGCCCTTAAAACGTACCAAAAAAACTTCAATCTTGTTGCCTCTGGAATTTTGGACGCCAACACATTAGCTCAAATGGGGAAGCCACGATGTGGGGTTGAAGATGTTATAAATGGCACAACTTGGATGAGATCGGGAAGGGAAAGGAAGCCAAAAATGCATGCTCATTTTCACTTAGTGTCTCACTTTGCTTTCTTTGAAGGAAACCTCAAATGGCCGCCTTCAAAGTTCCACTTGACCTATGCATTTCTTCCAGGGTATCCCTTTGAGGCAATGATGGCAGTGTCGAGGGCTTTTTCCAAGTGGGCTTTCAATACCCACTTCACATTTTCACGTGTTTTTTACCATACAAAAGCTGATATCAAAATAAGCTTTGAAATAGGAGACCATGGAGATGATGTGCCGTTTGATGGTGCGGGGGGGATTCTGGCGCATGCTTATGCGCCCACGGACGGCAGGCTTCACTTTGATGGCGATGAGGCTTGGTCTGTGGGAGCTGTTGATGGTTATTTTGATGTGGAGACTATTGCGTTGCATGAGATTGGACACGTTCTAGGGCTTCAACATAGCTCCATTGAAAACGCTATTATGTTCCCAAGCATATCGGAGGGAGTTACTAAGGGTTTGCATGGGGATGACATAGCTGGGATTAGGGCTCTATATAAAGTTTGA

Coding sequence (CDS)

ATGGCTTCCAAACCCCTTCAACTCCTCGCTTTCACCCTTCTCCTCCCTTCCCTCTTCCCTCGTTTCGACATCGTGTCGGTCCAATGCCACTCAGAATTGGAAGCTGTGGGCTCATCTCCGATCATTTTGAGTCCTAATTCCCACGCCCCGAACTCGTCCTCCTTGTTGTTTCTCAACAATCTCCAAGGATGTCGTTTGGGTGACACAGCCCAAGGTATTCATCGGATAAAAAAGTACCTTCAACGCTTTGGTTACATTACCAATGTCCAAAAACAATCCAACGCCATGGATCATGATGACACCTTTGATAATGCCTTGGAATCCGCCCTTAAAACGTACCAAAAAAACTTCAATCTTGTTGCCTCTGGAATTTTGGACGCCAACACATTAGCTCAAATGGGGAAGCCACGATGTGGGGTTGAAGATGTTATAAATGGCACAACTTGGATGAGATCGGGAAGGGAAAGGAAGCCAAAAATGCATGCTCATTTTCACTTAGTGTCTCACTTTGCTTTCTTTGAAGGAAACCTCAAATGGCCGCCTTCAAAGTTCCACTTGACCTATGCATTTCTTCCAGGGTATCCCTTTGAGGCAATGATGGCAGTGTCGAGGGCTTTTTCCAAGTGGGCTTTCAATACCCACTTCACATTTTCACGTGTTTTTTACCATACAAAAGCTGATATCAAAATAAGCTTTGAAATAGGAGACCATGGAGATGATGTGCCGTTTGATGGTGCGGGGGGGATTCTGGCGCATGCTTATGCGCCCACGGACGGCAGGCTTCACTTTGATGGCGATGAGGCTTGGTCTGTGGGAGCTGTTGATGGTTATTTTGATGTGGAGACTATTGCGTTGCATGAGATTGGACACGTTCTAGGGCTTCAACATAGCTCCATTGAAAACGCTATTATGTTCCCAAGCATATCGGAGGGAGTTACTAAGGGTTTGCATGGGGATGACATAGCTGGGATTAGGGCTCTATATAAAGTTTGA

Protein sequence

MASKPLQLLAFTLLLPSLFPRFDIVSVQCHSELEAVGSSPIILSPNSHAPNSSSLLFLNNLQGCRLGDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALESALKTYQKNFNLVASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKMHAHFHLVSHFAFFEGNLKWPPSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVFYHTKADIKISFEIGDHGDDVPFDGAGGILAHAYAPTDGRLHFDGDEAWSVGAVDGYFDVETIALHEIGHVLGLQHSSIENAIMFPSISEGVTKGLHGDDIAGIRALYKV
Homology
BLAST of Csor.00g121250 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 3.4e-58
Identity = 122/283 (43.11%), Postives = 169/283 (59.72%), Query Frame = 0

Query: 59  NNLQGCRLGDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALESALKTYQKNFN 118
           +N  GC  G    G++RIKKY QRFGYI     ++ + +  D FD+ L++A++ YQ NFN
Sbjct: 45  SNFTGCHHGQNVDGLYRIKKYFQRFGYI----PETFSGNFTDDFDDILKAAVELYQTNFN 104

Query: 119 LVASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKMH---AHFHLVSHFAFFEG 178
           L  +G LDA T+  +  PRCG  DV+NGT+ M  GR +  +++    H H V  +  F G
Sbjct: 105 LNVTGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPG 164

Query: 179 NLKWPPSKFHLTYAFLPGYPF--EAMMAVSRAFSKWAFNTHFTFSRVFYHTKADIKISFE 238
             +WP ++  LTYAF P  P   E     SRAF +W+  T   F+     + +DI I F 
Sbjct: 165 EPRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFY 224

Query: 239 IGDHGDDVPFDGAGGILAHAYAPTDGRLHFDGDEAWSV-GAVDGYF------DVETIALH 298
            GDHGD  PFDG  G LAHA++P  G+ H D DE W V G +D +       D+E++A+H
Sbjct: 225 TGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVH 284

Query: 299 EIGHVLGLQHSSIENAIMFPSISEGVTK-GLHGDDIAGIRALY 329
           EIGH+LGL HSS+E +IM+P+I+ G  K  L  DD+ GI+ LY
Sbjct: 285 EIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

BLAST of Csor.00g121250 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 2.2e-57
Identity = 126/282 (44.68%), Postives = 165/282 (58.51%), Query Frame = 0

Query: 59  NNLQGCRLGDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALESALKTYQKNFN 118
           + L GC +G+   G+ ++K+Y +RFGYIT     +      D FD+ L+SA+ TYQKNFN
Sbjct: 46  SKLAGCHIGENINGLSKLKQYFRRFGYITTTGNCT------DDFDDVLQSAINTYQKNFN 105

Query: 119 LVASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKMHAHFHLVSHFAFFEGNLK 178
           L  +G LD++TL Q+ KPRCG  D+I+G + M  G+               ++FF G  +
Sbjct: 106 LKVTGKLDSSTLRQIVKPRCGNPDLIDGVSEMNGGK--------ILRTTEKYSFFPGKPR 165

Query: 179 WPPSKFHLTYAFLP--GYPFEAMMAVSRAFSKWAFNTHFTFSRVFYHTKADIKISFEIGD 238
           WP  K  LTYAF P      E     SRAF++WA  T   F+R     +ADI I F  G+
Sbjct: 166 WPKRKRDLTYAFAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGE 225

Query: 239 HGDDVPFDGAGGILAHAYAPTDGRLHFDGDEAWSVG---------AVDGYFDVETIALHE 298
           HGD  PFDGA G LAHA +P  G LH DGDE W +           V    D+E++A+HE
Sbjct: 226 HGDGEPFDGAMGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHE 285

Query: 299 IGHVLGLQHSSIENAIMFPSISEGVTK-GLHGDDIAGIRALY 329
           IGH+LGL HSS+E+AIMFP+IS G  K  L  DDI GI+ LY
Sbjct: 286 IGHLLGLGHSSVEDAIMFPAISGGDRKVELAKDDIEGIQHLY 313

BLAST of Csor.00g121250 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 2.9e-57
Identity = 122/284 (42.96%), Postives = 165/284 (58.10%), Query Frame = 0

Query: 60  NLQGCRLGDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALESALKTYQKNFNL 119
           N  GC  G    G++ +K+Y Q FGYIT      N     D FD+ L++A++ YQ+NF L
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYITETNLSGN---FTDDFDDILKNAVEMYQRNFQL 107

Query: 120 VASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKMHA----HFHLVSHFAFFEG 179
             +G+LD  TL  +  PRCG  DV+NGT+ M SGR+      A     FH V H++FF G
Sbjct: 108 NVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPG 167

Query: 180 NLKWPPSKFHLTYAFLP--GYPFEAMMAVSRAFSKWAFNTHFTFSRVFYHTKADIKISFE 239
             +WP ++  LTYAF P      E     SRAF++W   T  TF+RV   + +DI I F 
Sbjct: 168 EPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFY 227

Query: 240 IGDHGDDVPFDGAGGILAHAYAPTDGRLHFDGDEAWSVGAV--DGYF------DVETIAL 299
            G+HGD  PFDG    LAHA++P  G  H DG+E W V     DG+       D+E++A+
Sbjct: 228 SGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAV 287

Query: 300 HEIGHVLGLQHSSIENAIMFPSISEGVTK-GLHGDDIAGIRALY 329
           HEIGH+LGL HSS+E +IM+P+I  G  K  L  DD+ G++ LY
Sbjct: 288 HEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

BLAST of Csor.00g121250 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 209.1 bits (531), Expect = 7.4e-53
Identity = 120/273 (43.96%), Postives = 150/273 (54.95%), Query Frame = 0

Query: 67  GDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALESALKTYQKNFNLVASGILD 126
           G   +G+  +K Y    GYI       NA   DD FD+ L SA+KTYQKN+NL  +G  D
Sbjct: 48  GQNYKGLSNVKNYFHHLGYI------PNAPHFDDNFDDTLVSAIKTYQKNYNLNVTGKFD 107

Query: 127 ANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKMHAHFHLVSHFAFFEGNLKWPPSKFHL 186
            NTL Q+  PRCGV D+I  T    S           F ++S + FF+   +W      L
Sbjct: 108 INTLKQIMTPRCGVPDIIINTNKTTS-----------FGMISDYTFFKDMPRWQAGTTQL 167

Query: 187 TYAFLPGYPFEAMM--AVSRAFSKWAFNTHFTFSRVFYHTKADIKISFEIGDHGDDVPFD 246
           TYAF P    +     A++RAFSKW    +  F     +  A+IKI F   +HGD  PFD
Sbjct: 168 TYAFSPEPRLDDTFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFD 227

Query: 247 GAGGILAHAYAPTDGRLHFDGDEAW------SVGAVDGYFDVETIALHEIGHVLGLQHSS 306
           G GGIL HA+APTDGR HFD DE W      +   V   FD+E++A+HEIGH+LGL HSS
Sbjct: 228 GPGGILGHAFAPTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSS 287

Query: 307 IENAIMFPSISEGVTK-GLHGDDIAGIRALYKV 331
              AIM+PSI     K  L  DDI GIR LY +
Sbjct: 288 DLRAIMYPSIPPRTRKVNLAQDDIDGIRKLYGI 303

BLAST of Csor.00g121250 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 5.1e-46
Identity = 113/293 (38.57%), Postives = 157/293 (53.58%), Query Frame = 0

Query: 48  HAPNSSSLLFLNNLQGCRLGDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALE 107
           H  NS+   F + L   ++G    G+  +K+YL RFGY+ +      +    D FD  LE
Sbjct: 46  HVSNSTWHDF-SRLVDVQIGSHVSGVSELKRYLHRFGYVND-----GSEIFSDVFDGPLE 105

Query: 108 SALKTYQKNFNLVASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKMHAHFHLV 167
           SA+  YQ+N  L  +G LD +T+  M  PRCGV D     T M    +         H  
Sbjct: 106 SAISLYQENLGLPITGRLDTSTVTLMSLPRCGVSD-----THMTINND-------FLHTT 165

Query: 168 SHFAFFEGNLKWPPSKFHLTYAFLPGYPFEAMMA------VSRAFSKWAFNTHFTFSRVF 227
           +H+ +F G  KW  ++  LTYA    +  + + +        RAFS+W+     +F  V 
Sbjct: 166 AHYTYFNGKPKW--NRDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVD 225

Query: 228 YHTKADIKISFEIGDHGDDVPFDGAGGILAHAYAPTDGRLHFDGDEAWSV-----GAVDG 287
             T AD+KI F  GDHGD +PFDG  G LAHA+AP +GRLH D  E W V     G+ + 
Sbjct: 226 DFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEV 285

Query: 288 YFDVETIALHEIGHVLGLQHSSIENAIMFPSISEGVTK-GLHGDDIAGIRALY 329
             D+E++A HEIGH+LGL HSS E+A+M+PS+     K  L  DD+AG+  LY
Sbjct: 286 AVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

BLAST of Csor.00g121250 vs. NCBI nr
Match: KAG6602462.1 (Metalloendoproteinase 2-MMP, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 673 bits (1736), Expect = 3.44e-244
Identity = 330/330 (100.00%), Postives = 330/330 (100.00%), Query Frame = 0

Query: 1   MASKPLQLLAFTLLLPSLFPRFDIVSVQCHSELEAVGSSPIILSPNSHAPNSSSLLFLNN 60
           MASKPLQLLAFTLLLPSLFPRFDIVSVQCHSELEAVGSSPIILSPNSHAPNSSSLLFLNN
Sbjct: 1   MASKPLQLLAFTLLLPSLFPRFDIVSVQCHSELEAVGSSPIILSPNSHAPNSSSLLFLNN 60

Query: 61  LQGCRLGDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALESALKTYQKNFNLV 120
           LQGCRLGDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALESALKTYQKNFNLV
Sbjct: 61  LQGCRLGDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALESALKTYQKNFNLV 120

Query: 121 ASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKMHAHFHLVSHFAFFEGNLKWP 180
           ASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKMHAHFHLVSHFAFFEGNLKWP
Sbjct: 121 ASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKMHAHFHLVSHFAFFEGNLKWP 180

Query: 181 PSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVFYHTKADIKISFEIGDHGDD 240
           PSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVFYHTKADIKISFEIGDHGDD
Sbjct: 181 PSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVFYHTKADIKISFEIGDHGDD 240

Query: 241 VPFDGAGGILAHAYAPTDGRLHFDGDEAWSVGAVDGYFDVETIALHEIGHVLGLQHSSIE 300
           VPFDGAGGILAHAYAPTDGRLHFDGDEAWSVGAVDGYFDVETIALHEIGHVLGLQHSSIE
Sbjct: 241 VPFDGAGGILAHAYAPTDGRLHFDGDEAWSVGAVDGYFDVETIALHEIGHVLGLQHSSIE 300

Query: 301 NAIMFPSISEGVTKGLHGDDIAGIRALYKV 330
           NAIMFPSISEGVTKGLHGDDIAGIRALYKV
Sbjct: 301 NAIMFPSISEGVTKGLHGDDIAGIRALYKV 330

BLAST of Csor.00g121250 vs. NCBI nr
Match: XP_022962668.1 (metalloendoproteinase 2-MMP-like [Cucurbita moschata])

HSP 1 Score: 654 bits (1687), Expect = 1.02e-236
Identity = 322/330 (97.58%), Postives = 323/330 (97.88%), Query Frame = 0

Query: 1   MASKPLQLLAFTLLLPSLFPRFDIVSVQCHSELEAVGSSPIILSPNSHAPNSSSLLFLNN 60
           MASKPLQLLAFTLLLPS FP FDIVSVQCHSE EAVGSSPIIL+ NSHA NSSSLLFLNN
Sbjct: 1   MASKPLQLLAFTLLLPSFFPHFDIVSVQCHSESEAVGSSPIILNHNSHATNSSSLLFLNN 60

Query: 61  LQGCRLGDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALESALKTYQKNFNLV 120
           LQGCRLGDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALESALKTYQKNFNLV
Sbjct: 61  LQGCRLGDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALESALKTYQKNFNLV 120

Query: 121 ASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKMHAHFHLVSHFAFFEGNLKWP 180
           ASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKMHAHFHLVSHFAFFEGNLKWP
Sbjct: 121 ASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKMHAHFHLVSHFAFFEGNLKWP 180

Query: 181 PSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVFYHTKADIKISFEIGDHGDD 240
           PSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVF HTKADIKISFEIGDHGDD
Sbjct: 181 PSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVFDHTKADIKISFEIGDHGDD 240

Query: 241 VPFDGAGGILAHAYAPTDGRLHFDGDEAWSVGAVDGYFDVETIALHEIGHVLGLQHSSIE 300
           VPFDG GGILAHAYAPTDGRLHFDGDEAWSVGAVDGYFDVETIALHEIGHVLGLQHSSIE
Sbjct: 241 VPFDGVGGILAHAYAPTDGRLHFDGDEAWSVGAVDGYFDVETIALHEIGHVLGLQHSSIE 300

Query: 301 NAIMFPSISEGVTKGLHGDDIAGIRALYKV 330
           NAIMFPSISEGVTKGLHGDDIAGIRALYKV
Sbjct: 301 NAIMFPSISEGVTKGLHGDDIAGIRALYKV 330

BLAST of Csor.00g121250 vs. NCBI nr
Match: XP_022991021.1 (metalloendoproteinase 2-MMP-like [Cucurbita maxima])

HSP 1 Score: 639 bits (1649), Expect = 6.30e-231
Identity = 312/330 (94.55%), Postives = 317/330 (96.06%), Query Frame = 0

Query: 1   MASKPLQLLAFTLLLPSLFPRFDIVSVQCHSELEAVGSSPIILSPNSHAPNSSSLLFLNN 60
           MASKPLQLLAFTLLLPS FP FDI SVQCHSE + VGSSPIIL+ NSHA NS+SLLFLNN
Sbjct: 1   MASKPLQLLAFTLLLPSFFPHFDIASVQCHSESKVVGSSPIILNHNSHATNSASLLFLNN 60

Query: 61  LQGCRLGDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALESALKTYQKNFNLV 120
           LQGCRLGDT QGIHRIKKYLQRFGYI+NVQK SNAMDHDDTFDN LESALKTYQKNFNLV
Sbjct: 61  LQGCRLGDTVQGIHRIKKYLQRFGYISNVQKHSNAMDHDDTFDNTLESALKTYQKNFNLV 120

Query: 121 ASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKMHAHFHLVSHFAFFEGNLKWP 180
           ASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRER PKMHAHFHLVSHFAFFEGNLKWP
Sbjct: 121 ASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERNPKMHAHFHLVSHFAFFEGNLKWP 180

Query: 181 PSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVFYHTKADIKISFEIGDHGDD 240
           PSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVF HTKADIKISFEIGDHGDD
Sbjct: 181 PSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVFDHTKADIKISFEIGDHGDD 240

Query: 241 VPFDGAGGILAHAYAPTDGRLHFDGDEAWSVGAVDGYFDVETIALHEIGHVLGLQHSSIE 300
            PFDGAGGILAHAYAPTDGRLHFDGDEAWSVGAVDGYFDVET+ALHEIGHVLGLQHSSIE
Sbjct: 241 APFDGAGGILAHAYAPTDGRLHFDGDEAWSVGAVDGYFDVETVALHEIGHVLGLQHSSIE 300

Query: 301 NAIMFPSISEGVTKGLHGDDIAGIRALYKV 330
           NAIMFPSISEGVTKGLHGDDIAGIRALYKV
Sbjct: 301 NAIMFPSISEGVTKGLHGDDIAGIRALYKV 330

BLAST of Csor.00g121250 vs. NCBI nr
Match: XP_023552591.1 (metalloendoproteinase 5-MMP-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 638 bits (1646), Expect = 1.74e-230
Identity = 315/330 (95.45%), Postives = 321/330 (97.27%), Query Frame = 0

Query: 1   MASKPLQLLAFTLLLPSLFPRFDIVSVQCHSELEAVGSSPIILSPNSHAPNSSSLLFLNN 60
           MASKPLQLLAFTLLLPSLFPRFDI SVQCHSE EAVGSSPIIL+ NSHA N+SSL FL N
Sbjct: 1   MASKPLQLLAFTLLLPSLFPRFDIASVQCHSESEAVGSSPIILNHNSHATNTSSL-FLKN 60

Query: 61  LQGCRLGDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALESALKTYQKNFNLV 120
           LQGCRLGDTAQGIHRIKKYLQRFGYITNVQK SNAMDHDDTFDNALESA+KTYQKNFNLV
Sbjct: 61  LQGCRLGDTAQGIHRIKKYLQRFGYITNVQKHSNAMDHDDTFDNALESAVKTYQKNFNLV 120

Query: 121 ASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKMHAHFHLVSHFAFFEGNLKWP 180
           ASGILDANTLAQMGKPRCGVEDVINGTTWMR GRERKPKMHAHFHLV+HFAFFEGNLKWP
Sbjct: 121 ASGILDANTLAQMGKPRCGVEDVINGTTWMRWGRERKPKMHAHFHLVAHFAFFEGNLKWP 180

Query: 181 PSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVFYHTKADIKISFEIGDHGDD 240
           PSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVF HTKADIKISFEIGDHGDD
Sbjct: 181 PSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVFDHTKADIKISFEIGDHGDD 240

Query: 241 VPFDGAGGILAHAYAPTDGRLHFDGDEAWSVGAVDGYFDVETIALHEIGHVLGLQHSSIE 300
           VPFDGAGGILAHAYAPTDGRLHFDGDEAWSVGAVDGYFDVETIALHEIGHVLGLQHSSIE
Sbjct: 241 VPFDGAGGILAHAYAPTDGRLHFDGDEAWSVGAVDGYFDVETIALHEIGHVLGLQHSSIE 300

Query: 301 NAIMFPSISEGVTKGLHGDDIAGIRALYKV 330
           NAIMFPSISEGVTKGLHGDDIAGIRALY++
Sbjct: 301 NAIMFPSISEGVTKGLHGDDIAGIRALYQL 329

BLAST of Csor.00g121250 vs. NCBI nr
Match: XP_022134170.1 (metalloendoproteinase 2-MMP-like [Momordica charantia])

HSP 1 Score: 460 bits (1184), Expect = 3.02e-160
Identity = 239/332 (71.99%), Postives = 263/332 (79.22%), Query Frame = 0

Query: 1   MASKPLQLLAFTL-LLPSLFPRFDIVSVQCHSELEAVGSSPIILSPNS--HAPNSSSLLF 60
           MASK LQ+ AF L LL S FP  +            + S  +IL  N+  H  NSSS L 
Sbjct: 1   MASKALQIFAFILFLLLSFFPHLN------------MSSHVLILKQNAIAHDMNSSSSLS 60

Query: 61  LNNLQGCRLGDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALESALKTYQKNF 120
            NNL+GCRLGDTAQGIHR+KKYL+RFGYITNV+K S AMD DDTFD+ALESA+KTYQKN+
Sbjct: 61  FNNLRGCRLGDTAQGIHRLKKYLRRFGYITNVEKNSMAMD-DDTFDHALESAVKTYQKNY 120

Query: 121 NLVASGILDANTLAQMGKPRCGVEDVINGTTWMRS-GRERKPKMHAHFHLVSHFAFFEGN 180
           N+ ASGILDA T+ QMG PRCGV+D INGTTWMRS GR R    H+HFH VSHFAFFEGN
Sbjct: 121 NIDASGILDAKTITQMGMPRCGVQDFINGTTWMRSSGRRRNQNGHSHFHKVSHFAFFEGN 180

Query: 181 LKWPPSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVFYHTKADIKISFEIGD 240
           LKWP SKFHL+YAFLPGYP EA++ VSRAFSKWA NTHFTFS V  H  ADIKISFEI +
Sbjct: 181 LKWPASKFHLSYAFLPGYPSEAILPVSRAFSKWASNTHFTFSHVSDHKTADIKISFEIEE 240

Query: 241 HGDDVPFDGAGGILAHAYAPTDGRLHFDGDEAWSVGAVDGYFDVETIALHEIGHVLGLQH 300
           HGDD  FDGAGGILAHAY PTDGR H D  E WSVGAV G+ DVET+ALHEIGH+LGLQH
Sbjct: 241 HGDDAAFDGAGGILAHAYPPTDGRFHLDAHERWSVGAVSGHHDVETVALHEIGHLLGLQH 300

Query: 301 SSIENAIMFPSISEGVTKGLHGDDIAGIRALY 328
           SSIE  IMFPSISEGVTKGLHGDDIAGI+ALY
Sbjct: 301 SSIEGPIMFPSISEGVTKGLHGDDIAGIKALY 319

BLAST of Csor.00g121250 vs. ExPASy TrEMBL
Match: A0A6J1HFG1 (metalloendoproteinase 2-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111463086 PE=3 SV=1)

HSP 1 Score: 654 bits (1687), Expect = 4.92e-237
Identity = 322/330 (97.58%), Postives = 323/330 (97.88%), Query Frame = 0

Query: 1   MASKPLQLLAFTLLLPSLFPRFDIVSVQCHSELEAVGSSPIILSPNSHAPNSSSLLFLNN 60
           MASKPLQLLAFTLLLPS FP FDIVSVQCHSE EAVGSSPIIL+ NSHA NSSSLLFLNN
Sbjct: 1   MASKPLQLLAFTLLLPSFFPHFDIVSVQCHSESEAVGSSPIILNHNSHATNSSSLLFLNN 60

Query: 61  LQGCRLGDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALESALKTYQKNFNLV 120
           LQGCRLGDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALESALKTYQKNFNLV
Sbjct: 61  LQGCRLGDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALESALKTYQKNFNLV 120

Query: 121 ASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKMHAHFHLVSHFAFFEGNLKWP 180
           ASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKMHAHFHLVSHFAFFEGNLKWP
Sbjct: 121 ASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKMHAHFHLVSHFAFFEGNLKWP 180

Query: 181 PSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVFYHTKADIKISFEIGDHGDD 240
           PSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVF HTKADIKISFEIGDHGDD
Sbjct: 181 PSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVFDHTKADIKISFEIGDHGDD 240

Query: 241 VPFDGAGGILAHAYAPTDGRLHFDGDEAWSVGAVDGYFDVETIALHEIGHVLGLQHSSIE 300
           VPFDG GGILAHAYAPTDGRLHFDGDEAWSVGAVDGYFDVETIALHEIGHVLGLQHSSIE
Sbjct: 241 VPFDGVGGILAHAYAPTDGRLHFDGDEAWSVGAVDGYFDVETIALHEIGHVLGLQHSSIE 300

Query: 301 NAIMFPSISEGVTKGLHGDDIAGIRALYKV 330
           NAIMFPSISEGVTKGLHGDDIAGIRALYKV
Sbjct: 301 NAIMFPSISEGVTKGLHGDDIAGIRALYKV 330

BLAST of Csor.00g121250 vs. ExPASy TrEMBL
Match: A0A6J1JPK1 (metalloendoproteinase 2-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111487736 PE=3 SV=1)

HSP 1 Score: 639 bits (1649), Expect = 3.05e-231
Identity = 312/330 (94.55%), Postives = 317/330 (96.06%), Query Frame = 0

Query: 1   MASKPLQLLAFTLLLPSLFPRFDIVSVQCHSELEAVGSSPIILSPNSHAPNSSSLLFLNN 60
           MASKPLQLLAFTLLLPS FP FDI SVQCHSE + VGSSPIIL+ NSHA NS+SLLFLNN
Sbjct: 1   MASKPLQLLAFTLLLPSFFPHFDIASVQCHSESKVVGSSPIILNHNSHATNSASLLFLNN 60

Query: 61  LQGCRLGDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALESALKTYQKNFNLV 120
           LQGCRLGDT QGIHRIKKYLQRFGYI+NVQK SNAMDHDDTFDN LESALKTYQKNFNLV
Sbjct: 61  LQGCRLGDTVQGIHRIKKYLQRFGYISNVQKHSNAMDHDDTFDNTLESALKTYQKNFNLV 120

Query: 121 ASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKMHAHFHLVSHFAFFEGNLKWP 180
           ASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRER PKMHAHFHLVSHFAFFEGNLKWP
Sbjct: 121 ASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERNPKMHAHFHLVSHFAFFEGNLKWP 180

Query: 181 PSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVFYHTKADIKISFEIGDHGDD 240
           PSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVF HTKADIKISFEIGDHGDD
Sbjct: 181 PSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVFDHTKADIKISFEIGDHGDD 240

Query: 241 VPFDGAGGILAHAYAPTDGRLHFDGDEAWSVGAVDGYFDVETIALHEIGHVLGLQHSSIE 300
            PFDGAGGILAHAYAPTDGRLHFDGDEAWSVGAVDGYFDVET+ALHEIGHVLGLQHSSIE
Sbjct: 241 APFDGAGGILAHAYAPTDGRLHFDGDEAWSVGAVDGYFDVETVALHEIGHVLGLQHSSIE 300

Query: 301 NAIMFPSISEGVTKGLHGDDIAGIRALYKV 330
           NAIMFPSISEGVTKGLHGDDIAGIRALYKV
Sbjct: 301 NAIMFPSISEGVTKGLHGDDIAGIRALYKV 330

BLAST of Csor.00g121250 vs. ExPASy TrEMBL
Match: A0A6J1BXD5 (metalloendoproteinase 2-MMP-like OS=Momordica charantia OX=3673 GN=LOC111006498 PE=3 SV=1)

HSP 1 Score: 460 bits (1184), Expect = 1.46e-160
Identity = 239/332 (71.99%), Postives = 263/332 (79.22%), Query Frame = 0

Query: 1   MASKPLQLLAFTL-LLPSLFPRFDIVSVQCHSELEAVGSSPIILSPNS--HAPNSSSLLF 60
           MASK LQ+ AF L LL S FP  +            + S  +IL  N+  H  NSSS L 
Sbjct: 1   MASKALQIFAFILFLLLSFFPHLN------------MSSHVLILKQNAIAHDMNSSSSLS 60

Query: 61  LNNLQGCRLGDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALESALKTYQKNF 120
            NNL+GCRLGDTAQGIHR+KKYL+RFGYITNV+K S AMD DDTFD+ALESA+KTYQKN+
Sbjct: 61  FNNLRGCRLGDTAQGIHRLKKYLRRFGYITNVEKNSMAMD-DDTFDHALESAVKTYQKNY 120

Query: 121 NLVASGILDANTLAQMGKPRCGVEDVINGTTWMRS-GRERKPKMHAHFHLVSHFAFFEGN 180
           N+ ASGILDA T+ QMG PRCGV+D INGTTWMRS GR R    H+HFH VSHFAFFEGN
Sbjct: 121 NIDASGILDAKTITQMGMPRCGVQDFINGTTWMRSSGRRRNQNGHSHFHKVSHFAFFEGN 180

Query: 181 LKWPPSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVFYHTKADIKISFEIGD 240
           LKWP SKFHL+YAFLPGYP EA++ VSRAFSKWA NTHFTFS V  H  ADIKISFEI +
Sbjct: 181 LKWPASKFHLSYAFLPGYPSEAILPVSRAFSKWASNTHFTFSHVSDHKTADIKISFEIEE 240

Query: 241 HGDDVPFDGAGGILAHAYAPTDGRLHFDGDEAWSVGAVDGYFDVETIALHEIGHVLGLQH 300
           HGDD  FDGAGGILAHAY PTDGR H D  E WSVGAV G+ DVET+ALHEIGH+LGLQH
Sbjct: 241 HGDDAAFDGAGGILAHAYPPTDGRFHLDAHERWSVGAVSGHHDVETVALHEIGHLLGLQH 300

Query: 301 SSIENAIMFPSISEGVTKGLHGDDIAGIRALY 328
           SSIE  IMFPSISEGVTKGLHGDDIAGI+ALY
Sbjct: 301 SSIEGPIMFPSISEGVTKGLHGDDIAGIKALY 319

BLAST of Csor.00g121250 vs. ExPASy TrEMBL
Match: A0A5D3BM88 (Metalloendoproteinase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1582G00220 PE=3 SV=1)

HSP 1 Score: 437 bits (1123), Expect = 2.41e-151
Identity = 226/332 (68.07%), Postives = 255/332 (76.81%), Query Frame = 0

Query: 2   ASKPLQLL-AFTLLLPSLFPRFDIVSVQCHSELEAVGSSPIILSPNSHAPNSS-SLLFLN 61
           +SK +Q++  FTLL  SLFP  +              SSP+IL  NSH  NSS SL+FL 
Sbjct: 3   SSKAIQIIFPFTLLFLSLFPNLNT-------------SSPMILKHNSHNMNSSNSLMFLK 62

Query: 62  NLQGCRLGDTAQGIHRIKKYLQRFGYIT-NVQKQSNAMDHDDTFDNALESALKTYQKNFN 121
           NLQGC LGDT QGI +IKKYLQRFGYIT N+QK SN +  DDTFD  LESALKTYQ N N
Sbjct: 63  NLQGCHLGDTKQGIQQIKKYLQRFGYITTNIQKHSNPI-FDDTFDQVLESALKTYQTNHN 122

Query: 122 LVASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKMHAHFHLVSHFAFFEGNLK 181
           L  SGILD+NTLAQ+ KPRCGV+DVI      +  ++ K   H HFH VSHF FFEGNLK
Sbjct: 123 LAPSGILDSNTLAQIAKPRCGVQDVIKNK---KRNQKYKNNGHIHFHKVSHFTFFEGNLK 182

Query: 182 WPPSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVFYHTKADIKISFEIGDHG 241
           WP SKFHL+Y FLP YP EA+  VSRAFSKW+ NTHF FS V  + KADIKISFE G+HG
Sbjct: 183 WPSSKFHLSYGFLPNYPIEAIKPVSRAFSKWSLNTHFKFSHVVDYRKADIKISFERGEHG 242

Query: 242 DDVPFDGAGGILAHAYAPTDGRLHFDGDEAWSVGAVDGYFDVETIALHEIGHVLGLQHSS 301
           D+ PFDG GG+LAHAY PTDGRLHFDGDEAWSVGA+ GYFDVET+ALHEIGH+LGLQHS+
Sbjct: 243 DNAPFDGVGGVLAHAYPPTDGRLHFDGDEAWSVGAISGYFDVETVALHEIGHILGLQHST 302

Query: 302 IENAIMFPSISEGVTKGLHGDDIAGIRALYKV 330
           IE AIMFPSI EGVTKGLHGDDIAGI+ LY+V
Sbjct: 303 IEEAIMFPSIPEGVTKGLHGDDIAGIKTLYRV 317

BLAST of Csor.00g121250 vs. ExPASy TrEMBL
Match: A0A1S3C814 (metalloendoproteinase 1-like OS=Cucumis melo OX=3656 GN=LOC103498042 PE=3 SV=1)

HSP 1 Score: 436 bits (1122), Expect = 3.42e-151
Identity = 226/332 (68.07%), Postives = 254/332 (76.51%), Query Frame = 0

Query: 2   ASKPLQLL-AFTLLLPSLFPRFDIVSVQCHSELEAVGSSPIILSPNSHAPNSS-SLLFLN 61
           +SK +Q++  FTLL  SLFP  +              SSP+IL  NSH  NSS SL+FL 
Sbjct: 3   SSKAIQIIFPFTLLFLSLFPNLNT-------------SSPMILKHNSHNMNSSNSLMFLK 62

Query: 62  NLQGCRLGDTAQGIHRIKKYLQRFGYIT-NVQKQSNAMDHDDTFDNALESALKTYQKNFN 121
           NLQGC LGDT QGI +IKKYLQRFGYIT N+QK SN +  DDTFD  LESALKTYQ N N
Sbjct: 63  NLQGCHLGDTKQGIQQIKKYLQRFGYITTNIQKHSNPI-FDDTFDQVLESALKTYQTNHN 122

Query: 122 LVASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKMHAHFHLVSHFAFFEGNLK 181
           L  SGILD+NTLAQ+ KPRCGV+DVI      +  +  K   H HFH VSHF FFEGNLK
Sbjct: 123 LAPSGILDSNTLAQIAKPRCGVQDVIKNK---KRNQNYKNNGHIHFHKVSHFTFFEGNLK 182

Query: 182 WPPSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVFYHTKADIKISFEIGDHG 241
           WP SKFHL+Y FLP YP EA+  VSRAFSKW+ NTHF FS V  + KADIKISFE G+HG
Sbjct: 183 WPSSKFHLSYGFLPNYPIEAIKPVSRAFSKWSLNTHFKFSHVVDYRKADIKISFERGEHG 242

Query: 242 DDVPFDGAGGILAHAYAPTDGRLHFDGDEAWSVGAVDGYFDVETIALHEIGHVLGLQHSS 301
           D+ PFDG GG+LAHAY PTDGRLHFDGDEAWSVGA+ GYFDVET+ALHEIGH+LGLQHS+
Sbjct: 243 DNAPFDGVGGVLAHAYPPTDGRLHFDGDEAWSVGAISGYFDVETVALHEIGHILGLQHST 302

Query: 302 IENAIMFPSISEGVTKGLHGDDIAGIRALYKV 330
           IE AIMFPSI EGVTKGLHGDDIAGI+ LY+V
Sbjct: 303 IEEAIMFPSIPEGVTKGLHGDDIAGIKTLYRV 317

BLAST of Csor.00g121250 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 226.9 bits (577), Expect = 2.4e-59
Identity = 122/283 (43.11%), Postives = 169/283 (59.72%), Query Frame = 0

Query: 59  NNLQGCRLGDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALESALKTYQKNFN 118
           +N  GC  G    G++RIKKY QRFGYI     ++ + +  D FD+ L++A++ YQ NFN
Sbjct: 45  SNFTGCHHGQNVDGLYRIKKYFQRFGYI----PETFSGNFTDDFDDILKAAVELYQTNFN 104

Query: 119 LVASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKMH---AHFHLVSHFAFFEG 178
           L  +G LDA T+  +  PRCG  DV+NGT+ M  GR +  +++    H H V  +  F G
Sbjct: 105 LNVTGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPG 164

Query: 179 NLKWPPSKFHLTYAFLPGYPF--EAMMAVSRAFSKWAFNTHFTFSRVFYHTKADIKISFE 238
             +WP ++  LTYAF P  P   E     SRAF +W+  T   F+     + +DI I F 
Sbjct: 165 EPRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFY 224

Query: 239 IGDHGDDVPFDGAGGILAHAYAPTDGRLHFDGDEAWSV-GAVDGYF------DVETIALH 298
            GDHGD  PFDG  G LAHA++P  G+ H D DE W V G +D +       D+E++A+H
Sbjct: 225 TGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVH 284

Query: 299 EIGHVLGLQHSSIENAIMFPSISEGVTK-GLHGDDIAGIRALY 329
           EIGH+LGL HSS+E +IM+P+I+ G  K  L  DD+ GI+ LY
Sbjct: 285 EIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

BLAST of Csor.00g121250 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 224.2 bits (570), Expect = 1.6e-58
Identity = 126/282 (44.68%), Postives = 165/282 (58.51%), Query Frame = 0

Query: 59  NNLQGCRLGDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALESALKTYQKNFN 118
           + L GC +G+   G+ ++K+Y +RFGYIT     +      D FD+ L+SA+ TYQKNFN
Sbjct: 46  SKLAGCHIGENINGLSKLKQYFRRFGYITTTGNCT------DDFDDVLQSAINTYQKNFN 105

Query: 119 LVASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKMHAHFHLVSHFAFFEGNLK 178
           L  +G LD++TL Q+ KPRCG  D+I+G + M  G+               ++FF G  +
Sbjct: 106 LKVTGKLDSSTLRQIVKPRCGNPDLIDGVSEMNGGK--------ILRTTEKYSFFPGKPR 165

Query: 179 WPPSKFHLTYAFLP--GYPFEAMMAVSRAFSKWAFNTHFTFSRVFYHTKADIKISFEIGD 238
           WP  K  LTYAF P      E     SRAF++WA  T   F+R     +ADI I F  G+
Sbjct: 166 WPKRKRDLTYAFAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGE 225

Query: 239 HGDDVPFDGAGGILAHAYAPTDGRLHFDGDEAWSVG---------AVDGYFDVETIALHE 298
           HGD  PFDGA G LAHA +P  G LH DGDE W +           V    D+E++A+HE
Sbjct: 226 HGDGEPFDGAMGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHE 285

Query: 299 IGHVLGLQHSSIENAIMFPSISEGVTK-GLHGDDIAGIRALY 329
           IGH+LGL HSS+E+AIMFP+IS G  K  L  DDI GI+ LY
Sbjct: 286 IGHLLGLGHSSVEDAIMFPAISGGDRKVELAKDDIEGIQHLY 313

BLAST of Csor.00g121250 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 223.8 bits (569), Expect = 2.1e-58
Identity = 122/284 (42.96%), Postives = 165/284 (58.10%), Query Frame = 0

Query: 60  NLQGCRLGDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALESALKTYQKNFNL 119
           N  GC  G    G++ +K+Y Q FGYIT      N     D FD+ L++A++ YQ+NF L
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYITETNLSGN---FTDDFDDILKNAVEMYQRNFQL 107

Query: 120 VASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKMHA----HFHLVSHFAFFEG 179
             +G+LD  TL  +  PRCG  DV+NGT+ M SGR+      A     FH V H++FF G
Sbjct: 108 NVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPG 167

Query: 180 NLKWPPSKFHLTYAFLP--GYPFEAMMAVSRAFSKWAFNTHFTFSRVFYHTKADIKISFE 239
             +WP ++  LTYAF P      E     SRAF++W   T  TF+RV   + +DI I F 
Sbjct: 168 EPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFY 227

Query: 240 IGDHGDDVPFDGAGGILAHAYAPTDGRLHFDGDEAWSVGAV--DGYF------DVETIAL 299
            G+HGD  PFDG    LAHA++P  G  H DG+E W V     DG+       D+E++A+
Sbjct: 228 SGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAV 287

Query: 300 HEIGHVLGLQHSSIENAIMFPSISEGVTK-GLHGDDIAGIRALY 329
           HEIGH+LGL HSS+E +IM+P+I  G  K  L  DD+ G++ LY
Sbjct: 288 HEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

BLAST of Csor.00g121250 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 186.4 bits (472), Expect = 3.6e-47
Identity = 113/293 (38.57%), Postives = 157/293 (53.58%), Query Frame = 0

Query: 48  HAPNSSSLLFLNNLQGCRLGDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALE 107
           H  NS+   F + L   ++G    G+  +K+YL RFGY+ +      +    D FD  LE
Sbjct: 46  HVSNSTWHDF-SRLVDVQIGSHVSGVSELKRYLHRFGYVND-----GSEIFSDVFDGPLE 105

Query: 108 SALKTYQKNFNLVASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKMHAHFHLV 167
           SA+  YQ+N  L  +G LD +T+  M  PRCGV D     T M    +         H  
Sbjct: 106 SAISLYQENLGLPITGRLDTSTVTLMSLPRCGVSD-----THMTINND-------FLHTT 165

Query: 168 SHFAFFEGNLKWPPSKFHLTYAFLPGYPFEAMMA------VSRAFSKWAFNTHFTFSRVF 227
           +H+ +F G  KW  ++  LTYA    +  + + +        RAFS+W+     +F  V 
Sbjct: 166 AHYTYFNGKPKW--NRDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVD 225

Query: 228 YHTKADIKISFEIGDHGDDVPFDGAGGILAHAYAPTDGRLHFDGDEAWSV-----GAVDG 287
             T AD+KI F  GDHGD +PFDG  G LAHA+AP +GRLH D  E W V     G+ + 
Sbjct: 226 DFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEV 285

Query: 288 YFDVETIALHEIGHVLGLQHSSIENAIMFPSISEGVTK-GLHGDDIAGIRALY 329
             D+E++A HEIGH+LGL HSS E+A+M+PS+     K  L  DD+AG+  LY
Sbjct: 286 AVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

BLAST of Csor.00g121250 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 162.9 bits (411), Expect = 4.3e-40
Identity = 103/268 (38.43%), Postives = 144/268 (53.73%), Query Frame = 0

Query: 73  IHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALESALKTYQKNFNLVASGILDANTLAQ 132
           I  IK++LQ++GY+    K+S+        D + E AL  YQKN  L  +G  D++TL+Q
Sbjct: 50  IPEIKRHLQQYGYLPQ-NKESD--------DVSFEQALVRYQKNLGLPITGKPDSDTLSQ 109

Query: 133 MGKPRCGVEDVINGTTWMRSGRERKPKMHAHFHLVSHFAFFEGNLKWPPS-KFHLTYAF- 192
           +  PRCG  D +   T             A FH    + +F G  +W       LTYAF 
Sbjct: 110 ILLPRCGFPDDVEPKT-------------APFHTGKKYVYFPGRPRWTRDVPLKLTYAFS 169

Query: 193 ---LPGY--PFEAMMAVSRAFSKWAFNTHFTFSRVFYHTKADIKISFEIGDHGDDVPFDG 252
              L  Y  P +      RAF KWA     +F     +  ADIKI F  GDHGD  PFDG
Sbjct: 170 QENLTPYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDG 229

Query: 253 AGGILAHAYAPTDGRLHFDGDEAWSVGAVDG----YFDVETIALHEIGHVLGLQHSSIEN 312
             G+LAH ++P +GRLH D  E W+V   +       D+E++A+HEIGHVLGL HSS+++
Sbjct: 230 VLGVLAHTFSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKD 289

Query: 313 AIMFPSISEGVTK-GLHGDDIAGIRALY 329
           A M+P++     K  L+ DD+ G+++LY
Sbjct: 290 AAMYPTLKPRSKKVNLNMDDVVGVQSLY 295

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O045293.4e-5843.11Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1[more]
Q9ZUJ52.2e-5744.68Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1[more]
Q5XF512.9e-5742.96Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1[more]
P291367.4e-5343.96Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2[more]
O235075.1e-4638.57Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAG6602462.13.44e-244100.00Metalloendoproteinase 2-MMP, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022962668.11.02e-23697.58metalloendoproteinase 2-MMP-like [Cucurbita moschata][more]
XP_022991021.16.30e-23194.55metalloendoproteinase 2-MMP-like [Cucurbita maxima][more]
XP_023552591.11.74e-23095.45metalloendoproteinase 5-MMP-like [Cucurbita pepo subsp. pepo][more]
XP_022134170.13.02e-16071.99metalloendoproteinase 2-MMP-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A6J1HFG14.92e-23797.58metalloendoproteinase 2-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111463086 P... [more]
A0A6J1JPK13.05e-23194.55metalloendoproteinase 2-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111487736 PE=... [more]
A0A6J1BXD51.46e-16071.99metalloendoproteinase 2-MMP-like OS=Momordica charantia OX=3673 GN=LOC111006498 ... [more]
A0A5D3BM882.41e-15168.07Metalloendoproteinase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3C8143.42e-15168.07metalloendoproteinase 1-like OS=Cucumis melo OX=3656 GN=LOC103498042 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G70170.12.4e-5943.11matrix metalloproteinase [more]
AT1G59970.11.6e-5844.68Matrixin family protein [more]
AT1G24140.12.1e-5842.96Matrixin family protein [more]
AT4G16640.13.6e-4738.57Matrixin family protein [more]
AT2G45040.14.3e-4038.43Matrixin family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021190Peptidase M10APRINTSPR00138MATRIXINcoord: 226..254
score: 61.61
coord: 316..329
score: 52.96
coord: 133..146
score: 55.84
coord: 202..217
score: 34.6
coord: 283..308
score: 52.91
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 175..330
e-value: 8.9E-28
score: 108.3
IPR001818Peptidase M10, metallopeptidasePFAMPF00413Peptidase_M10coord: 197..328
e-value: 1.6E-42
score: 145.1
IPR002477Peptidoglycan binding-likePFAMPF01471PG_binding_1coord: 71..133
e-value: 3.2E-14
score: 52.9
IPR024079Metallopeptidase, catalytic domain superfamilyGENE3D3.40.390.10Collagenase (Catalytic Domain)coord: 65..330
e-value: 9.3E-72
score: 243.4
NoneNo IPR availablePANTHERPTHR10201:SF269METALLOENDOPROTEINASE 3-MMP-LIKEcoord: 46..330
NoneNo IPR availablePANTHERPTHR10201MATRIX METALLOPROTEINASEcoord: 46..330
NoneNo IPR availableSUPERFAMILY55486Metalloproteases ("zincins"), catalytic domaincoord: 172..329
IPR033739Peptidase M10A, catalytic domainCDDcd04278ZnMc_MMPcoord: 178..328
e-value: 2.19943E-59
score: 186.256
IPR036365PGBD-like superfamilySUPERFAMILY47090PGBD-likecoord: 64..143

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g121250.m01Csor.00g121250.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030574 collagen catabolic process
biological_process GO:0030198 extracellular matrix organization
biological_process GO:0006508 proteolysis
cellular_component GO:0031225 anchored component of membrane
cellular_component GO:0031012 extracellular matrix
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0008237 metallopeptidase activity