Csor.00g120220 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g120220
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionCytochrome b561 domain-containing protein
LocationCsor_Chr04: 20342985 .. 20346020 (+)
RNA-Seq ExpressionCsor.00g120220
SyntenyCsor.00g120220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGCTGAAATTGGCTTCGAATTTGCCCACGATTTCCTCCATTGAAGATCCTAATACTCCAATTCTTTCCCCCACTAACTCATCAACTGAAGAACCAATGGCGGTTCCCGCGGTTCAGTTCCCGATCTTCATGGCCGTGAGAGTGCTCGGGATGATTGTGGCAACTCTCGTCTTCATTTGGACCTTGCACTTCCGTGGAGGATTGGCGCTTATATCAGATAACAAGGATCTCATCTTCAATGTAATTAGCAATTTCCTCTGTATTCACAAATTATTTTGCTCTTCCGTGTGTTGTTCTTTTCCCTCTTTTTTCCGATTTCTGAATTGATGCGCATGTTTGATGGGTCTTGAGTTAATTTAGTTTTCCGTGGTCTTTCTGCTTCGGATTTTGGATTTGGGTGTTTGAAATTTGAGCACCATTTGGCTTGTCACCAGCAGAGATGTTTGGAATGAAATTAACGGTGCTTACTACTTGCTCACCATTGTTAGTTCTTCTCAGCTTCAGCCTGAAATGGCTGTTTAATGTACTCGTGTAGTTTTGAATTGTATGCTCTAAACCCATTGATGATGCATGCGTAGAGTAATTGGGTCTATTATTTCTGGGGTTTGGAGTATTGGTGACAAATTTAGAGATGAATTTTGAATGGCTGGTCATGTCTGGTGGGCAAAAGAAGTCATATGCATATAGAAATCTTTTACTGTCAGCCATCTGTGATCTGCATTTCTTTTCAGTTGCAAAAAGATGGGAGATAATGCAAAATAGAATTCATTCTTTACATGTGGCTCCAATCATCAAGGTCAATAGAATTTTTTGTTATTGACACAGCTTAGCTCGAGTAACATTTGGCTATCTACTTTTATTTCTTGTGCCTCTTATATGAATCTAGTAACAAGCATGGTTCAATCTTAGTATCTAAATTATTTTTTAATGCGTATCTTCATAGAATTGTTTATTTTATATTGATGGTAATCCTAGTGGAGAGTGTTTGTGTGAGATCCCACGCCGGTTGGGGAGGAGAATGAAGCATTCTTTACAAGGGTGTGGAAACCTCTCCCTAGTAGACACATTTTAAAAACCTAGTTCGAAAAGGAAAGCCCAAAGAGAACAATATCTGCTAGCGGTGGGTCTGGGCTGGGCTGTTACAGTTTGTTTGCTCCCATTCTAGAACTCAACTTAAATAATGCATCCGATGGTCTTGTACGTTTATAAGTTTGGTCTTGTAGTGGATAGAACCCAAGGATATGAGCATAGTATTGAAATGGTATTGTCATGAAGACTTTTGGATCTAATGATGTCACGCGAGGGAAACATGATTATTATCTTTGCTGTTGATATATTCTATATGCTTTACATAATAGAAACTTTAATTTTTAATTCTTTTTGTCCTTTTATTTTCCTGTTTATTTGGTGAAGTTGCTCCTGTTTTGTAGGTCCATCCTGTTCTTATGGTCATTGGACTCATACTTCTAAATGGTGAAGGTAAATATCTTTTTTCTTTGGACTTAAATTGTAGTTTGAAGCACTAGTTTACACACGTTTGTGTTCTTCTTCCATTGTGGTTTTGATGCGATGGTTTGTATTTGACTATTTGAATGCATATGTAGACTGTGAAAATATGATGCTACTATATTAAAAGTGAGAAATCACGAATTCTTTTTTTATGGGAAACAAAATGCATTTGATAAGAGATATGAAATTACAAAAAATAAGGGGGGAGGAAAGGCAAATCACGTTTGATATAAATAAGTGTTTTTGTGGGCTACGGGTGGAGGGTTATGAGATCGTTCACTCAATCCCGGGGAAAGAACTCCTTGAGCTTTAGTTGGAAGCTAAACCAAAGCTAAGCTTGTAGGCTCGCAGAGAAAGTCATTTTACAGGTATATCCCTGAACAAGCCAAGAAAGTTATGAAAACCTCTTTCAGTTGGCACAAAGATAAGAAGGTTGTAAAAAAAAAAAAAGGCTCTTACTAAAGAGCAAGTATTTGCCTTTCTCTAAAGAGACATAACAGCGTTGGAGAGTTGGAACTAGTAGATTGTAATTTAAGTGGCTCTTTTGATCTAGAACATTCAATGTGCTCAATTTTTTCTTCCCTGGGTGGTGGCGTGTAGAGTTGTATGAGAGAGATTGGTACTGTTGTTACTAGTTACTACTGAATGGTTATTGCTTACTGAATGGTTTTTGTTGTTGAGAAAACTGGGATAATGGGCAAGGATCTTAACAGTATAAAACATTTGCTAGGCTTTGTCACGTAGGTCTTAGGTTTTGTACAGGAAACGTGTTATTTTACAAAACGTGCTAGAGGTCAGGTTTAGAATCATGTATGAAATATGTATCCTGAAATCATTCACAAACTTGAGTTAAAGTTAATTCTCCTACCATTCTGGTGCGTTCAATGGTTTTGTTCACCGTGCTTAGATGTATTGATGCAGCAATGTTGGCATACAAGACAGTCTCAGGAACCAAAAATTTCAGAAAACTAGTTCATCTTGCAGTACAGTTTGTTGCATTCTGTTTAAGCCTGATTGGCATATGGGCTGCCCTGAAGTTCCACAACGACAAAGGAATCGAGAACTTTTACAGTTTGCATTCATGGTTGGGTCTAGTTTCGATCTTTCTGTTCGGCTTCCAGGTTCGTAAATTGCAATTTCTGCTGGTGTGATGGTATGATTTATTTTTTGAGCCCGCTTGTTGAGCTATAACTCATTAGGATGACACCATTTGAACAGTGGACTGCAGGATTTGTAACCTTTTGGTACCCAGGCGGCTCCAGAAACAGCAGATCGACTCTATTGCCATGGCATGTTTTCTTTGGTGTTTACATATATGGCCTTTCCATTGCTACAGCTGTTACTGGCTTGTTAGAAAAAGCTACATTTCTTCAAGTTAGCAAGGTGATTTTACGCTATTCCAACGAGGCGTTGCTGTTAAATTCGTTGGGTCTCCTCATTGTTCTTCTGGGTGGTCTTGTAATTCTCGCGGTTATCGCTCCATCGAATGGCAAAGGTGACATTTTCAGGGGGCCATCAGAATAG

mRNA sequence

ATGCAGCTGAAATTGGCTTCGAATTTGCCCACGATTTCCTCCATTGAAGATCCTAATACTCCAATTCTTTCCCCCACTAACTCATCAACTGAAGAACCAATGGCGGTTCCCGCGGTTCAGTTCCCGATCTTCATGGCCGTGAGAGTGCTCGGGATGATTGTGGCAACTCTCGTCTTCATTTGGACCTTGCACTTCCGTGGAGGATTGGCGCTTATATCAGATAACAAGGATCTCATCTTCAATGTCCATCCTGTTCTTATGGTCATTGGACTCATACTTCTAAATGGTGAAGCAATGTTGGCATACAAGACAGTCTCAGGAACCAAAAATTTCAGAAAACTAGTTCATCTTGCAGTACAGTTTGTTGCATTCTGTTTAAGCCTGATTGGCATATGGGCTGCCCTGAAGTTCCACAACGACAAAGGAATCGAGAACTTTTACAGTTTGCATTCATGGTTGGGTCTAGTTTCGATCTTTCTGTTCGGCTTCCAGTGGACTGCAGGATTTGTAACCTTTTGGTACCCAGGCGGCTCCAGAAACAGCAGATCGACTCTATTGCCATGGCATGTTTTCTTTGGTGTTTACATATATGGCCTTTCCATTGCTACAGCTGTTACTGGCTTGTTAGAAAAAGCTACATTTCTTCAAGTTAGCAAGGTGATTTTACGCTATTCCAACGAGGCGTTGCTGTTAAATTCGTTGGGTCTCCTCATTGTTCTTCTGGGTGGTCTTGTAATTCTCGCGGTTATCGCTCCATCGAATGGCAAAGGTGACATTTTCAGGGGGCCATCAGAATAG

Coding sequence (CDS)

ATGCAGCTGAAATTGGCTTCGAATTTGCCCACGATTTCCTCCATTGAAGATCCTAATACTCCAATTCTTTCCCCCACTAACTCATCAACTGAAGAACCAATGGCGGTTCCCGCGGTTCAGTTCCCGATCTTCATGGCCGTGAGAGTGCTCGGGATGATTGTGGCAACTCTCGTCTTCATTTGGACCTTGCACTTCCGTGGAGGATTGGCGCTTATATCAGATAACAAGGATCTCATCTTCAATGTCCATCCTGTTCTTATGGTCATTGGACTCATACTTCTAAATGGTGAAGCAATGTTGGCATACAAGACAGTCTCAGGAACCAAAAATTTCAGAAAACTAGTTCATCTTGCAGTACAGTTTGTTGCATTCTGTTTAAGCCTGATTGGCATATGGGCTGCCCTGAAGTTCCACAACGACAAAGGAATCGAGAACTTTTACAGTTTGCATTCATGGTTGGGTCTAGTTTCGATCTTTCTGTTCGGCTTCCAGTGGACTGCAGGATTTGTAACCTTTTGGTACCCAGGCGGCTCCAGAAACAGCAGATCGACTCTATTGCCATGGCATGTTTTCTTTGGTGTTTACATATATGGCCTTTCCATTGCTACAGCTGTTACTGGCTTGTTAGAAAAAGCTACATTTCTTCAAGTTAGCAAGGTGATTTTACGCTATTCCAACGAGGCGTTGCTGTTAAATTCGTTGGGTCTCCTCATTGTTCTTCTGGGTGGTCTTGTAATTCTCGCGGTTATCGCTCCATCGAATGGCAAAGGTGACATTTTCAGGGGGCCATCAGAATAG

Protein sequence

MQLKLASNLPTISSIEDPNTPILSPTNSSTEEPMAVPAVQFPIFMAVRVLGMIVATLVFIWTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEAMLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSNGKGDIFRGPSE
Homology
BLAST of Csor.00g120220 vs. ExPASy Swiss-Prot
Match: Q9SWS1 (Transmembrane ascorbate ferrireductase 2 OS=Arabidopsis thaliana OX=3702 GN=CYB561B PE=1 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 6.1e-90
Identity = 161/227 (70.93%), Postives = 191/227 (84.14%), Query Frame = 0

Query: 34  MAVPAV-QFPIFMAVRVLGMIVATLVFIWTLHFRGGLALISDNKDLIFNVHPVLMVIGLI 93
           MAVP +  FPIFM VRVLG I+A LV  WT+H+RGGLAL SDNKD IFNVHPV+MVIGLI
Sbjct: 1   MAVPVLGGFPIFMVVRVLGFIIAALVLTWTVHYRGGLALSSDNKDHIFNVHPVMMVIGLI 60

Query: 94  LLNGEAMLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSW 153
           L NGEAMLAYK+V GTKN +KLVHL +Q  AF LSLIG+WAALKFH DKGIENFYSLHSW
Sbjct: 61  LFNGEAMLAYKSVQGTKNLKKLVHLTLQLTAFILSLIGVWAALKFHIDKGIENFYSLHSW 120

Query: 154 LGLVSIFLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKA 213
           LGL  +FLF FQW AGFVT+WYPGGSRNSR++L+PWHVF G+ IY L++ TA TG+LEK 
Sbjct: 121 LGLACLFLFAFQWAAGFVTYWYPGGSRNSRASLMPWHVFLGISIYALALVTATTGILEKV 180

Query: 214 TFLQVSKVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSNGKGDI 260
           TFLQV++VI RYS EA+L+N++G+LI++LGG VIL V+ P +GK  +
Sbjct: 181 TFLQVNQVITRYSTEAMLVNTMGVLILILGGFVILGVVTPVSGKDQV 227

BLAST of Csor.00g120220 vs. ExPASy Swiss-Prot
Match: Q7XMK3 (Probable ascorbate-specific transmembrane electron transporter 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0533500 PE=2 SV=2)

HSP 1 Score: 204.9 bits (520), Expect = 1.1e-51
Identity = 104/214 (48.60%), Postives = 142/214 (66.36%), Query Frame = 0

Query: 42  PIFMAVRVLGMIVATLVFIWTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEAMLA 101
           P       L +  A +V +W + FRGGLA  +DNK+LIFNVHPVLM+IG I+L  EA++ 
Sbjct: 11  PFTYVAHALAVAAAVMVLVWCISFRGGLAFEADNKNLIFNVHPVLMLIGYIILGSEAIMI 70

Query: 102 YKTVSG-TKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIFL 161
           YK       +  KL+HL +  +A  L  +GI+ A KFHN+ GI N YSLHSWLG+ +I L
Sbjct: 71  YKIFPKLNHDTTKLIHLILHAIAIVLGAVGIYCAFKFHNESGIANLYSLHSWLGIGTISL 130

Query: 162 FGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSKV 221
           +G QW  GFV F+YPG + + R   LPWHV FG+++Y L++ATA  GLLEK TFLQ S  
Sbjct: 131 YGIQWIFGFVAFFYPGAAPHVRRGALPWHVLFGLFVYVLTLATAELGLLEKLTFLQ-SSG 190

Query: 222 ILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSN 255
           + +Y  EA L+N  GL++ L G  V++A +AP++
Sbjct: 191 LDKYGAEAFLVNFTGLVVALFGAAVVVAAVAPAH 223

BLAST of Csor.00g120220 vs. ExPASy Swiss-Prot
Match: A3A9H6 (Probable ascorbate-specific transmembrane electron transporter 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0642300 PE=3 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 9.5e-51
Identity = 101/213 (47.42%), Postives = 142/213 (66.67%), Query Frame = 0

Query: 42  PIFMAVRVLGMIVATLVFIWTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEAMLA 101
           P       L +  AT+V +W +HFRGGLA  + NK+LIFNVHPVLM+IG I+L  EA++ 
Sbjct: 9   PFTYVAHALAVAAATMVLVWCIHFRGGLAFEATNKNLIFNVHPVLMLIGYIILGSEAIMV 68

Query: 102 YKTVSGTK-NFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIFL 161
           YK +   K +  KL+HL +  +A     +GI+ A KFHN+ GI N YSLHSWLG+ +I L
Sbjct: 69  YKVLPTWKHDTTKLIHLILHAIALVFGAVGIYCAFKFHNESGIANLYSLHSWLGIGTICL 128

Query: 162 FGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSKV 221
           +G QW  GFV F++P  S + R  +LPWH+ FG+++Y L++ATA  G LEK TFLQ S  
Sbjct: 129 YGIQWIFGFVAFFFPRASPSVRKGVLPWHILFGLFVYILALATAELGFLEKLTFLQ-SSG 188

Query: 222 ILRYSNEALLLNSLGLLIVLLGGLVILAVIAPS 254
           + +Y  EA L+N   L++VL G  V++A ++P+
Sbjct: 189 LDKYGAEAFLVNFTALIVVLFGASVVVAAVSPA 220

BLAST of Csor.00g120220 vs. ExPASy Swiss-Prot
Match: Q6I681 (Ascorbate-specific transmembrane electron transporter 1 OS=Zea mays OX=4577 GN=ZCYB PE=1 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 1.6e-50
Identity = 99/212 (46.70%), Postives = 144/212 (67.92%), Query Frame = 0

Query: 42  PIFMAVRVLGMIVATLVFIWTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEAMLA 101
           P   A   L +  A +V +W++ FRGGLA+ S NK+LIFNVHPVLM+IG +++ GEA++ 
Sbjct: 11  PFTYAAHALAVAAAAMVLVWSIQFRGGLAIESTNKNLIFNVHPVLMLIGYVIIGGEAIMV 70

Query: 102 YKTV-SGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIFL 161
           Y+ + +   +  KL+HL +  +A  L  +GI+ A K HN+ GI N YSLHSW+G+ +I L
Sbjct: 71  YRVLPTSNHDTTKLIHLILHGIALVLGAVGIYFAFKNHNESGIANLYSLHSWIGIGTITL 130

Query: 162 FGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSKV 221
           +G QW  GFVTF++PG + N +  +LPWHV FG+++Y L++A A  G LEK TFL+ S  
Sbjct: 131 YGIQWIIGFVTFFFPGAAPNVKKGVLPWHVLFGLFVYILALANAELGFLEKLTFLE-SSG 190

Query: 222 ILRYSNEALLLNSLGLLIVLLGGLVILAVIAP 253
           + +Y  EA L+N   L++VL G  V++A IAP
Sbjct: 191 LDKYGTEAFLVNFTALVVVLFGASVVVAAIAP 221

BLAST of Csor.00g120220 vs. ExPASy Swiss-Prot
Match: C4IYS8 (Ascorbate-specific transmembrane electron transporter 2 OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 1.6e-50
Identity = 98/212 (46.23%), Postives = 143/212 (67.45%), Query Frame = 0

Query: 42  PIFMAVRVLGMIVATLVFIWTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEAMLA 101
           P   A   L +  A +V +W ++FRGGLA+ + NK+LIFNVHPVLM+IG I++ GEA++ 
Sbjct: 11  PFTYAAHALAVAAAAMVLVWAIYFRGGLAIEATNKNLIFNVHPVLMLIGYIIIGGEAIMV 70

Query: 102 YKTV-SGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIFL 161
           Y+ + +      KL+HL +  +A  L  +GI+ A K HN+ GI N YSLHSW+G+ +I L
Sbjct: 71  YRVLPTSNHETNKLIHLVLHGIALVLGAVGIYFAFKNHNESGIANLYSLHSWIGIGTITL 130

Query: 162 FGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSKV 221
           +G QW  GFVTF++PG + N +  +LPWH+ FG+++Y L++A A  G LEK TFL+ S  
Sbjct: 131 YGIQWIVGFVTFFFPGAAPNVKKGVLPWHILFGLFVYILALANAELGFLEKLTFLE-SSG 190

Query: 222 ILRYSNEALLLNSLGLLIVLLGGLVILAVIAP 253
           + +Y  EA L+N   L++VL G  V++A IAP
Sbjct: 191 LDKYGTEAFLVNFTALVVVLFGASVVVAAIAP 221

BLAST of Csor.00g120220 vs. NCBI nr
Match: KAG6602565.1 (putative transmembrane ascorbate ferrireductase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 512 bits (1318), Expect = 1.17e-182
Identity = 265/265 (100.00%), Postives = 265/265 (100.00%), Query Frame = 0

Query: 1   MQLKLASNLPTISSIEDPNTPILSPTNSSTEEPMAVPAVQFPIFMAVRVLGMIVATLVFI 60
           MQLKLASNLPTISSIEDPNTPILSPTNSSTEEPMAVPAVQFPIFMAVRVLGMIVATLVFI
Sbjct: 1   MQLKLASNLPTISSIEDPNTPILSPTNSSTEEPMAVPAVQFPIFMAVRVLGMIVATLVFI 60

Query: 61  WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEAMLAYKTVSGTKNFRKLVHLAVQ 120
           WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEAMLAYKTVSGTKNFRKLVHLAVQ
Sbjct: 61  WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEAMLAYKTVSGTKNFRKLVHLAVQ 120

Query: 121 FVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRN 180
           FVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRN
Sbjct: 121 FVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRN 180

Query: 181 SRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVL 240
           SRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVL
Sbjct: 181 SRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVL 240

Query: 241 LGGLVILAVIAPSNGKGDIFRGPSE 265
           LGGLVILAVIAPSNGKGDIFRGPSE
Sbjct: 241 LGGLVILAVIAPSNGKGDIFRGPSE 265

BLAST of Csor.00g120220 vs. NCBI nr
Match: XP_022956458.1 (probable transmembrane ascorbate ferrireductase 2 [Cucurbita moschata])

HSP 1 Score: 507 bits (1305), Expect = 1.12e-180
Identity = 262/265 (98.87%), Postives = 264/265 (99.62%), Query Frame = 0

Query: 1   MQLKLASNLPTISSIEDPNTPILSPTNSSTEEPMAVPAVQFPIFMAVRVLGMIVATLVFI 60
           MQLKLASNLPTISSIEDPNTPILSPT SSTEEPMAVPAVQFPIFMAVRVLGM+VATLVF+
Sbjct: 1   MQLKLASNLPTISSIEDPNTPILSPTISSTEEPMAVPAVQFPIFMAVRVLGMVVATLVFM 60

Query: 61  WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEAMLAYKTVSGTKNFRKLVHLAVQ 120
           WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEAMLAYKTVSGTKNFRKLVHLAVQ
Sbjct: 61  WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEAMLAYKTVSGTKNFRKLVHLAVQ 120

Query: 121 FVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRN 180
           FVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRN
Sbjct: 121 FVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRN 180

Query: 181 SRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVL 240
           SRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVL
Sbjct: 181 SRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVL 240

Query: 241 LGGLVILAVIAPSNGKGDIFRGPSE 265
           LGGLVILAVIAPSNGKGDIFRGPSE
Sbjct: 241 LGGLVILAVIAPSNGKGDIFRGPSE 265

BLAST of Csor.00g120220 vs. NCBI nr
Match: KAG7033242.1 (putative transmembrane ascorbate ferrireductase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 506 bits (1303), Expect = 2.18e-180
Identity = 262/264 (99.24%), Postives = 263/264 (99.62%), Query Frame = 0

Query: 2   QLKLASNLPTISSIEDPNTPILSPTNSSTEEPMAVPAVQFPIFMAVRVLGMIVATLVFIW 61
           QLKLASNLPTISSIEDPNTPILSPT SSTEEPMAVPAVQFPIFMAVRVLGM+VATLVFIW
Sbjct: 1   QLKLASNLPTISSIEDPNTPILSPTISSTEEPMAVPAVQFPIFMAVRVLGMVVATLVFIW 60

Query: 62  TLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEAMLAYKTVSGTKNFRKLVHLAVQF 121
           TLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEAMLAYKTVSGTKNFRKLVHLAVQF
Sbjct: 61  TLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEAMLAYKTVSGTKNFRKLVHLAVQF 120

Query: 122 VAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRNS 181
           VAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRNS
Sbjct: 121 VAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRNS 180

Query: 182 RSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVLL 241
           RSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVLL
Sbjct: 181 RSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVLL 240

Query: 242 GGLVILAVIAPSNGKGDIFRGPSE 265
           GGLVILAVIAPSNGKGDIFRGPSE
Sbjct: 241 GGLVILAVIAPSNGKGDIFRGPSE 264

BLAST of Csor.00g120220 vs. NCBI nr
Match: XP_023541096.1 (probable transmembrane ascorbate ferrireductase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 501 bits (1289), Expect = 3.08e-178
Identity = 259/265 (97.74%), Postives = 262/265 (98.87%), Query Frame = 0

Query: 1   MQLKLASNLPTISSIEDPNTPILSPTNSSTEEPMAVPAVQFPIFMAVRVLGMIVATLVFI 60
           MQLKLASNLPTISSIEDPNTPILSPT SS EEPMAVPAVQFPIFMAVRVLGM+VATLVFI
Sbjct: 1   MQLKLASNLPTISSIEDPNTPILSPTISSAEEPMAVPAVQFPIFMAVRVLGMVVATLVFI 60

Query: 61  WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEAMLAYKTVSGTKNFRKLVHLAVQ 120
           WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEAMLAYKTVSGTKNFRKLVHLAVQ
Sbjct: 61  WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEAMLAYKTVSGTKNFRKLVHLAVQ 120

Query: 121 FVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRN 180
           FVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRN
Sbjct: 121 FVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRN 180

Query: 181 SRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVL 240
           SRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVL
Sbjct: 181 SRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVL 240

Query: 241 LGGLVILAVIAPSNGKGDIFRGPSE 265
           LGGLVILAVIAPS+ KGDIFRGP+E
Sbjct: 241 LGGLVILAVIAPSSSKGDIFRGPAE 265

BLAST of Csor.00g120220 vs. NCBI nr
Match: XP_022989930.1 (probable transmembrane ascorbate ferrireductase 2 [Cucurbita maxima])

HSP 1 Score: 497 bits (1279), Expect = 1.03e-176
Identity = 258/265 (97.36%), Postives = 261/265 (98.49%), Query Frame = 0

Query: 1   MQLKLASNLPTISSIEDPNTPILSPTNSSTEEPMAVPAVQFPIFMAVRVLGMIVATLVFI 60
           MQLKLASNLPTISSIEDPNTPILSPT SS EE MAVPAVQFPIFMAVRVLGM+VATLVFI
Sbjct: 1   MQLKLASNLPTISSIEDPNTPILSPTISSAEELMAVPAVQFPIFMAVRVLGMVVATLVFI 60

Query: 61  WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEAMLAYKTVSGTKNFRKLVHLAVQ 120
           WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEAMLAYKTVSGTKNFRKLVHLAVQ
Sbjct: 61  WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEAMLAYKTVSGTKNFRKLVHLAVQ 120

Query: 121 FVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRN 180
           FVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRN
Sbjct: 121 FVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRN 180

Query: 181 SRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVL 240
           SRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVL
Sbjct: 181 SRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVL 240

Query: 241 LGGLVILAVIAPSNGKGDIFRGPSE 265
           LGGLVILAVIAPS+ KGDIFRGP+E
Sbjct: 241 LGGLVILAVIAPSSSKGDIFRGPAE 265

BLAST of Csor.00g120220 vs. ExPASy TrEMBL
Match: A0A6J1GWW7 (probable transmembrane ascorbate ferrireductase 2 OS=Cucurbita moschata OX=3662 GN=LOC111458185 PE=4 SV=1)

HSP 1 Score: 507 bits (1305), Expect = 5.43e-181
Identity = 262/265 (98.87%), Postives = 264/265 (99.62%), Query Frame = 0

Query: 1   MQLKLASNLPTISSIEDPNTPILSPTNSSTEEPMAVPAVQFPIFMAVRVLGMIVATLVFI 60
           MQLKLASNLPTISSIEDPNTPILSPT SSTEEPMAVPAVQFPIFMAVRVLGM+VATLVF+
Sbjct: 1   MQLKLASNLPTISSIEDPNTPILSPTISSTEEPMAVPAVQFPIFMAVRVLGMVVATLVFM 60

Query: 61  WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEAMLAYKTVSGTKNFRKLVHLAVQ 120
           WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEAMLAYKTVSGTKNFRKLVHLAVQ
Sbjct: 61  WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEAMLAYKTVSGTKNFRKLVHLAVQ 120

Query: 121 FVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRN 180
           FVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRN
Sbjct: 121 FVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRN 180

Query: 181 SRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVL 240
           SRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVL
Sbjct: 181 SRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVL 240

Query: 241 LGGLVILAVIAPSNGKGDIFRGPSE 265
           LGGLVILAVIAPSNGKGDIFRGPSE
Sbjct: 241 LGGLVILAVIAPSNGKGDIFRGPSE 265

BLAST of Csor.00g120220 vs. ExPASy TrEMBL
Match: A0A6J1JNQ1 (probable transmembrane ascorbate ferrireductase 2 OS=Cucurbita maxima OX=3661 GN=LOC111486970 PE=4 SV=1)

HSP 1 Score: 497 bits (1279), Expect = 4.99e-177
Identity = 258/265 (97.36%), Postives = 261/265 (98.49%), Query Frame = 0

Query: 1   MQLKLASNLPTISSIEDPNTPILSPTNSSTEEPMAVPAVQFPIFMAVRVLGMIVATLVFI 60
           MQLKLASNLPTISSIEDPNTPILSPT SS EE MAVPAVQFPIFMAVRVLGM+VATLVFI
Sbjct: 1   MQLKLASNLPTISSIEDPNTPILSPTISSAEELMAVPAVQFPIFMAVRVLGMVVATLVFI 60

Query: 61  WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEAMLAYKTVSGTKNFRKLVHLAVQ 120
           WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEAMLAYKTVSGTKNFRKLVHLAVQ
Sbjct: 61  WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEAMLAYKTVSGTKNFRKLVHLAVQ 120

Query: 121 FVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRN 180
           FVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRN
Sbjct: 121 FVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRN 180

Query: 181 SRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVL 240
           SRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVL
Sbjct: 181 SRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVL 240

Query: 241 LGGLVILAVIAPSNGKGDIFRGPSE 265
           LGGLVILAVIAPS+ KGDIFRGP+E
Sbjct: 241 LGGLVILAVIAPSSSKGDIFRGPAE 265

BLAST of Csor.00g120220 vs. ExPASy TrEMBL
Match: A0A6J1BZR9 (probable transmembrane ascorbate ferrireductase 2 OS=Momordica charantia OX=3673 GN=LOC111006724 PE=4 SV=1)

HSP 1 Score: 420 bits (1079), Expect = 4.54e-147
Identity = 209/232 (90.09%), Postives = 222/232 (95.69%), Query Frame = 0

Query: 34  MAVPAVQFPIFMAVRVLGMIVATLVFIWTLHFRGGLALISDNKDLIFNVHPVLMVIGLIL 93
           MAVP VQFPIFMAVRVLGMIVATLV IWT HFRGGLALISDNKDLIFNVHPVLMVIGL+L
Sbjct: 1   MAVPVVQFPIFMAVRVLGMIVATLVIIWTSHFRGGLALISDNKDLIFNVHPVLMVIGLVL 60

Query: 94  LNGEAMLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWL 153
           LNGEAMLAYKT SGTKNFRKLVHL++QFVAFCLSLIG+WAALKFHNDKGI+NFYSLHSWL
Sbjct: 61  LNGEAMLAYKTASGTKNFRKLVHLSLQFVAFCLSLIGVWAALKFHNDKGIDNFYSLHSWL 120

Query: 154 GLVSIFLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKAT 213
           GL+S+FLFG QWTAGFVTFWYPGGSRNSR+TLLPWHVFFGVY YGLSIATA TGLLEKAT
Sbjct: 121 GLLSVFLFGIQWTAGFVTFWYPGGSRNSRATLLPWHVFFGVYTYGLSIATAATGLLEKAT 180

Query: 214 FLQVSKVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSNGKGDIFRGPSE 265
           FLQ SKVILRYSNEALL+NSLG+L+VLLGGLVILAVIAP NGKG+I RGP+E
Sbjct: 181 FLQTSKVILRYSNEALLINSLGILLVLLGGLVILAVIAPPNGKGEILRGPAE 232

BLAST of Csor.00g120220 vs. ExPASy TrEMBL
Match: A0A6J1K1L7 (probable transmembrane ascorbate ferrireductase 2 OS=Cucurbita maxima OX=3661 GN=LOC111489314 PE=4 SV=1)

HSP 1 Score: 412 bits (1058), Expect = 7.18e-144
Identity = 206/232 (88.79%), Postives = 221/232 (95.26%), Query Frame = 0

Query: 34  MAVPAVQFPIFMAVRVLGMIVATLVFIWTLHFRGGLALISDNKDLIFNVHPVLMVIGLIL 93
           MAVPAVQFPIFMAVRVLGM+VA LVFIWTLHFRGGLAL SDNKDLIFNVHPVLMVIGLIL
Sbjct: 1   MAVPAVQFPIFMAVRVLGMVVAALVFIWTLHFRGGLALSSDNKDLIFNVHPVLMVIGLIL 60

Query: 94  LNGEAMLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWL 153
           LNGEAMLAYKTVSGTKNF+KLVHLA+QFVA  LSLIGIWAALKFHNDKGI+NFYSLHSWL
Sbjct: 61  LNGEAMLAYKTVSGTKNFKKLVHLALQFVALFLSLIGIWAALKFHNDKGIDNFYSLHSWL 120

Query: 154 GLVSIFLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKAT 213
           GLVS+FLFG QW AGFVTFWYPGGSRNSR+TLLPWHVFFGVYIYGL IAT VTGLLEKAT
Sbjct: 121 GLVSVFLFGIQWGAGFVTFWYPGGSRNSRATLLPWHVFFGVYIYGLCIATTVTGLLEKAT 180

Query: 214 FLQVSKVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSNGKGDIFRGPSE 265
           FLQ SKVILRYSNEALL+NSLG+++V+LGGL+ILAVIA SN KGD++RGP+E
Sbjct: 181 FLQTSKVILRYSNEALLINSLGIVVVVLGGLIILAVIARSNNKGDVYRGPAE 232

BLAST of Csor.00g120220 vs. ExPASy TrEMBL
Match: Q3LGX4 (Cytochrome b561 OS=Citrullus lanatus OX=3654 GN=CLb561B PE=2 SV=1)

HSP 1 Score: 412 bits (1058), Expect = 7.18e-144
Identity = 209/232 (90.09%), Postives = 222/232 (95.69%), Query Frame = 0

Query: 34  MAVPAVQFPIFMAVRVLGMIVATLVFIWTLHFRGGLALISDNKDLIFNVHPVLMVIGLIL 93
           MAVP VQFPIFMAVRVLGM+VA L+FIWTLHFRGGLALIS+NKD IFNVHPVLMVIGLIL
Sbjct: 1   MAVPLVQFPIFMAVRVLGMLVAALLFIWTLHFRGGLALISENKDHIFNVHPVLMVIGLIL 60

Query: 94  LNGEAMLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWL 153
           LNGEAMLAYKTVSGTKNF+KLVHLA+QFVAF LSLIGIWAALKFHNDKGI+NFYSLHSWL
Sbjct: 61  LNGEAMLAYKTVSGTKNFKKLVHLALQFVAFFLSLIGIWAALKFHNDKGIDNFYSLHSWL 120

Query: 154 GLVSIFLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKAT 213
           GLVSIFLFG QW+AGFVTFWYPGGSRNSR+TLLPWHVFFG+YIYGLSIAT VTGLLEKAT
Sbjct: 121 GLVSIFLFGIQWSAGFVTFWYPGGSRNSRATLLPWHVFFGIYIYGLSIATTVTGLLEKAT 180

Query: 214 FLQVSKVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSNGKGDIFRGPSE 265
           FLQ SKVILRYSNEALL+NSLG+LIVLLGGLVILAVIAPSN KGD+ RG +E
Sbjct: 181 FLQTSKVILRYSNEALLINSLGVLIVLLGGLVILAVIAPSNAKGDVNRGLAE 232

BLAST of Csor.00g120220 vs. TAIR 10
Match: AT5G38630.1 (cytochrome B561-1 )

HSP 1 Score: 332.0 bits (850), Expect = 4.3e-91
Identity = 161/227 (70.93%), Postives = 191/227 (84.14%), Query Frame = 0

Query: 34  MAVPAV-QFPIFMAVRVLGMIVATLVFIWTLHFRGGLALISDNKDLIFNVHPVLMVIGLI 93
           MAVP +  FPIFM VRVLG I+A LV  WT+H+RGGLAL SDNKD IFNVHPV+MVIGLI
Sbjct: 1   MAVPVLGGFPIFMVVRVLGFIIAALVLTWTVHYRGGLALSSDNKDHIFNVHPVMMVIGLI 60

Query: 94  LLNGEAMLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSW 153
           L NGEAMLAYK+V GTKN +KLVHL +Q  AF LSLIG+WAALKFH DKGIENFYSLHSW
Sbjct: 61  LFNGEAMLAYKSVQGTKNLKKLVHLTLQLTAFILSLIGVWAALKFHIDKGIENFYSLHSW 120

Query: 154 LGLVSIFLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKA 213
           LGL  +FLF FQW AGFVT+WYPGGSRNSR++L+PWHVF G+ IY L++ TA TG+LEK 
Sbjct: 121 LGLACLFLFAFQWAAGFVTYWYPGGSRNSRASLMPWHVFLGISIYALALVTATTGILEKV 180

Query: 214 TFLQVSKVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSNGKGDI 260
           TFLQV++VI RYS EA+L+N++G+LI++LGG VIL V+ P +GK  +
Sbjct: 181 TFLQVNQVITRYSTEAMLVNTMGVLILILGGFVILGVVTPVSGKDQV 227

BLAST of Csor.00g120220 vs. TAIR 10
Match: AT4G25570.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 194.1 bits (492), Expect = 1.4e-49
Identity = 97/220 (44.09%), Postives = 142/220 (64.55%), Query Frame = 0

Query: 34  MAVPAVQFPIFMAVRVLGMIVATLVFIWTLHFRGGLALISDNKDLIFNVHPVLMVIGLIL 93
           MAV      +      L +I A +V +W++ +RGGLA  + NK+LIFN+HPVLM+IG I+
Sbjct: 1   MAVRINAMAVTFVAHALAVIAAIMVLVWSISYRGGLAWEATNKNLIFNLHPVLMLIGFII 60

Query: 94  LNGEAMLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWL 153
           L GEA+++YK++   K  +KL+HL +  +A  L + GI AA K HN+  I N YSLHSW+
Sbjct: 61  LGGEAIISYKSLPLEKPVKKLIHLILHAIALALGIFGICAAFKNHNESHIPNLYSLHSWI 120

Query: 154 GLVSIFLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKAT 213
           G+  I L+GFQW   F+ F++PGGS N +S LLPWH   G+++Y L++  A  G LEK T
Sbjct: 121 GIGVISLYGFQWVYSFIVFFFPGGSTNLKSGLLPWHAMLGLFVYILAVGNAALGFLEKLT 180

Query: 214 FLQVSKVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPS 254
           FL+ +  + +Y +EA L+N   ++ +L G  V+L   A S
Sbjct: 181 FLE-NGGLDKYGSEAFLINFTAIITILFGAFVVLTASAES 219

BLAST of Csor.00g120220 vs. TAIR 10
Match: AT1G26100.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 181.8 bits (460), Expect = 7.2e-46
Identity = 100/220 (45.45%), Postives = 144/220 (65.45%), Query Frame = 0

Query: 35  AVPAVQFPIFMAVRVLGMIVATLVFIWTLHFRGGLALISDNKDLIFN-VHPVLMVIGLIL 94
           +V   +  + +  R+ G++VA  V  W        AL   N  L ++ +HP+LMVIG IL
Sbjct: 3   SVDPSRLSLVLFARLSGLVVAVSVLYW--------ALFLPNLGLSYSTLHPLLMVIGFIL 62

Query: 95  LNGEAMLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGI-ENFYSLHSW 154
           ++GEA+L ++ + G++  +K VHL +Q +A   ++ GIW   KFH  +G+  NFYSLHSW
Sbjct: 63  VSGEAILIHRWLPGSRKTKKAVHLWLQGMALASAVFGIWT--KFHYQRGVFANFYSLHSW 122

Query: 155 LGLVSIFLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKA 214
           +GL+S+ LF  QW  GF++FW+ G  R +R+T LPWHVF G+Y YGL+IATA TGLLEK 
Sbjct: 123 MGLLSVSLFAAQWVTGFMSFWHRGEVRTTRTTFLPWHVFLGLYTYGLAIATAETGLLEKL 182

Query: 215 TFLQVSKVILRYSNEALLLNSLGLLIVLLGGLVILAVIAP 253
           TFLQ  + + R  +E++ +N LGL + LLG +VI A I P
Sbjct: 183 TFLQTKRNVPRRGSESMTVNGLGLGLALLGCIVITAAILP 212

BLAST of Csor.00g120220 vs. TAIR 10
Match: AT1G14730.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 144.8 bits (364), Expect = 9.7e-35
Identity = 77/205 (37.56%), Postives = 116/205 (56.59%), Query Frame = 0

Query: 45  MAVRVLGMIVATLVFIWTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEAMLAYKT 104
           +     G++   L+ IW LH+R G+   SDN   + NVHP LM  G + L G+AM+ YKT
Sbjct: 19  LVAHFFGILAVVLMLIWLLHYREGIEYGSDNPLKVLNVHPFLMYCGFLFLVGQAMMTYKT 78

Query: 105 VSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQ 164
              +   +K+VH  +  +   L ++GI AA +FH+   +++  SLHSW+GL +  L G Q
Sbjct: 79  AYASHQVQKMVHGGLHLIGLVLGIVGICAAFRFHDKVNLKDMVSLHSWIGLTTFILLGVQ 138

Query: 165 WTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSKVILRY 224
           W  G  TF  P  S  +R+ ++PWHV  G  +  + I  A+TGL+++AT L  S      
Sbjct: 139 WLFGAFTFLAPQSSSGTRTRMMPWHVLGGRALLYMGIVAALTGLMQRATMLGQST----- 198

Query: 225 SNEALLLNSLGLLIVLLGGLVILAV 250
           + E+ L+N LGL I+L G  V  +V
Sbjct: 199 NAESRLINFLGLAILLFGVSVDFSV 218

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SWS16.1e-9070.93Transmembrane ascorbate ferrireductase 2 OS=Arabidopsis thaliana OX=3702 GN=CYB5... [more]
Q7XMK31.1e-5148.60Probable ascorbate-specific transmembrane electron transporter 2 OS=Oryza sativa... [more]
A3A9H69.5e-5147.42Probable ascorbate-specific transmembrane electron transporter 1 OS=Oryza sativa... [more]
Q6I6811.6e-5046.70Ascorbate-specific transmembrane electron transporter 1 OS=Zea mays OX=4577 GN=Z... [more]
C4IYS81.6e-5046.23Ascorbate-specific transmembrane electron transporter 2 OS=Zea mays OX=4577 PE=2... [more]
Match NameE-valueIdentityDescription
KAG6602565.11.17e-182100.00putative transmembrane ascorbate ferrireductase 2, partial [Cucurbita argyrosper... [more]
XP_022956458.11.12e-18098.87probable transmembrane ascorbate ferrireductase 2 [Cucurbita moschata][more]
KAG7033242.12.18e-18099.24putative transmembrane ascorbate ferrireductase 2, partial [Cucurbita argyrosper... [more]
XP_023541096.13.08e-17897.74probable transmembrane ascorbate ferrireductase 2 [Cucurbita pepo subsp. pepo][more]
XP_022989930.11.03e-17697.36probable transmembrane ascorbate ferrireductase 2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1GWW75.43e-18198.87probable transmembrane ascorbate ferrireductase 2 OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1JNQ14.99e-17797.36probable transmembrane ascorbate ferrireductase 2 OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1BZR94.54e-14790.09probable transmembrane ascorbate ferrireductase 2 OS=Momordica charantia OX=3673... [more]
A0A6J1K1L77.18e-14488.79probable transmembrane ascorbate ferrireductase 2 OS=Cucurbita maxima OX=3661 GN... [more]
Q3LGX47.18e-14490.09Cytochrome b561 OS=Citrullus lanatus OX=3654 GN=CLb561B PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT5G38630.14.3e-9170.93cytochrome B561-1 [more]
AT4G25570.11.4e-4944.09Cytochrome b561/ferric reductase transmembrane protein family [more]
AT1G26100.17.2e-4645.45Cytochrome b561/ferric reductase transmembrane protein family [more]
AT1G14730.19.7e-3537.56Cytochrome b561/ferric reductase transmembrane protein family [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006593Cytochrome b561/ferric reductase transmembraneSMARTSM00665561_7coord: 82..208
e-value: 5.2E-52
score: 188.8
IPR006593Cytochrome b561/ferric reductase transmembranePFAMPF03188Cytochrom_B561coord: 82..214
e-value: 3.6E-46
score: 156.7
IPR006593Cytochrome b561/ferric reductase transmembranePROSITEPS50939CYTOCHROME_B561coord: 46..250
score: 46.575283
NoneNo IPR availableGENE3D1.20.120.1770coord: 34..262
e-value: 3.4E-88
score: 296.6
NoneNo IPR availablePANTHERPTHR10106:SF49B561, PUTATIVE-RELATEDcoord: 34..261
NoneNo IPR availableCDDcd08766Cyt_b561_ACYB-1_likecoord: 73..216
e-value: 3.18553E-78
score: 231.427
IPR043205Cytochrome b561/Cytochrome b reductase 1-likePANTHERPTHR10106CYTOCHROME B561-RELATEDcoord: 34..261

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g120220.m01Csor.00g120220.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016491 oxidoreductase activity