Csor.00g104350 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g104350
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
LocationCsor_Chr17: 7734161 .. 7735927 (-)
RNA-Seq ExpressionCsor.00g104350
SyntenyCsor.00g104350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGTCCGAAACACAAGCCAGAGTGCCCGTAATAGACTTGTCCAAATTAGAGAAGTTGGAGGCTGGCAGTGACGCATGGGGCTCAACATGCAAGGCGATGAGGGTTGGTTTGGAGGAATTTGGGTGCTTTTGTGTGGTGTTTGAGGAGGTTTCTTTGGAGCTTCATAACTCCTTCTTTGGAGCTGTTGATGAGCTGTTTCAGCTTCCAATTGAAACTCGAATCAAGAACACAAGTGAAAAGGCTTACCATGGCTACTTTGGCCGCTACTCCTTTCTTCCTCTCTATGAATCCATGGCTATTGATAACCCAACTCAGCTAGACGCTGCTCAAAGCTTTGCCAATCTCATGTGGCCGAATCAAGGAAACGATCGCTTCAGGTAACTTCTGAACTCGAATTCAACGTCTTAACAACTATGTAACTATGTTCATGTTTGAAATTGACTCGAAAAAAAAAGTTGAGTTATATGTTTAATTTATGAAGTTTGAACTCTGTGTTTATTTAGTTCATAAGCTTTAAAAATGTTTTGGTTGGTCGTAAACTTTTAATTTTGTGTTTAATAGGTTTGTAAATTTTTAGAAATTTTGATTAGGTGAAACATGGTGTTAACTCTTTACCACTTTGTGGTCTGAGGGAGCTATCTTATGTCACGTGAGTGTAATGGTTGGACGAGCGTTGTAAGATGAATGTCTTGGGCGTCTTCGTTTACAAATGGGTTTTTGAAAATAATGTCATAGGCACGGGTGTGTTGTGCCATGTAAAACTTTAGGATCAAATCGACACAAACTTGAAAGTTCATAAATTGTGAGATCCCACATCGGTTGAGGAGGAAAATGAAGTATTCCTTGTAAGGGTGTGGAAACCTTTCCCTAGAAGACACGTTTTAAAACCGGGCCAAAGTAGACAATATCTGCTAGCGATGGGCTTGAGCTGTTACAATTGATATCAAAGCCAGACACGAGACGGTGTGCCAGCGAGGATGCAGGTCTCCAAAGGGGGTAGATTGTGAGATTTTAAAATCCCAACATCGACTATCAGTGGACTTTAGTGTAAGGTGACTTACACTGTCAATTATGAACTTATAATCTAACCTAACGAAATCTTCCCTTAACGAAATCACAGCGAAACTATTCAACGTTTCTCAACCTTGGTGGCGAAACTGGACAAGATAGTAACAAGAATGGTGCTGGAAAGCTACGGAGTATCAAAGCAGCTGATCGACACAAACGTAGATTCAACAGCCAACCTTCTCCGCTTCTTCAAATACAGAGTTCCTGAGCAACACGAAACCGATGTGGGACTGCACGCCCATACAGATCTCACTTTTTTCTCCATAATTCATCAACATCTCATCGGTGGTCTCCAAATTCAATCGCTCGATGATCAATGGATCGACCTCGAGCCCTCCAGCCACTGCTCCTTCATCGTCATGGCGGGTGACGCGCTCATGGTAACCACACTCCATCATTCTGATTCATATCAATTATCGTTGAACTATACTCACTTTGTCTAAATAAACGTAGGCGTGGAGTAACGATAGAATAAGGAGTTGTAGGCATCGAGTGACGATGAAAGCGGAGGAAACGAGATACTCAGCGGGGATGTTTTCGTTCAAGAACGGAACGATTAGTGTGCCTGAAGAGCTTGTGGATGATGCCAACCCATTGCGATACAAGCCCTTTAATCACTATGACTTCCTTACTCATGACATAGCAAAGGGTTCTAAAAAATTTCTCTATCGTATTAGAGATTATTGCAGCATTTGA

mRNA sequence

ATGGGGTCCGAAACACAAGCCAGAGTGCCCGTAATAGACTTGTCCAAATTAGAGAAGTTGGAGGCTGGCAGTGACGCATGGGGCTCAACATGCAAGGCGATGAGGGTTGGTTTGGAGGAATTTGGGTGCTTTTGTGTGGTGTTTGAGGAGGTTTCTTTGGAGCTTCATAACTCCTTCTTTGGAGCTGTTGATGAGCTGTTTCAGCTTCCAATTGAAACTCGAATCAAGAACACAAGTGAAAAGGCTTACCATGGCTACTTTGGCCGCTACTCCTTTCTTCCTCTCTATGAATCCATGGCTATTGATAACCCAACTCAGCTAGACGCTGCTCAAAGCTTTGCCAATCTCATGTGGCCGAATCAAGGAAACGATCGCTTCAGCGAAACTATTCAACGTTTCTCAACCTTGGTGGCGAAACTGGACAAGATAGTAACAAGAATGGTGCTGGAAAGCTACGGAGTATCAAAGCAGCTGATCGACACAAACGTAGATTCAACAGCCAACCTTCTCCGCTTCTTCAAATACAGAGTTCCTGAGCAACACGAAACCGATGTGGGACTGCACGCCCATACAGATCTCACTTTTTTCTCCATAATTCATCAACATCTCATCGGTGGTCTCCAAATTCAATCGCTCGATGATCAATGGATCGACCTCGAGCCCTCCAGCCACTGCTCCTTCATCGTCATGGCGGGTGACGCGCTCATGGCGTGGAGTAACGATAGAATAAGGAGTTGTAGGCATCGAGTGACGATGAAAGCGGAGGAAACGAGATACTCAGCGGGGATGTTTTCGTTCAAGAACGGAACGATTAGTGTGCCTGAAGAGCTTGTGGATGATGCCAACCCATTGCGATACAAGCCCTTTAATCACTATGACTTCCTTACTCATGACATAGCAAAGGGTTCTAAAAAATTTCTCTATCGTATTAGAGATTATTGCAGCATTTGA

Coding sequence (CDS)

ATGGGGTCCGAAACACAAGCCAGAGTGCCCGTAATAGACTTGTCCAAATTAGAGAAGTTGGAGGCTGGCAGTGACGCATGGGGCTCAACATGCAAGGCGATGAGGGTTGGTTTGGAGGAATTTGGGTGCTTTTGTGTGGTGTTTGAGGAGGTTTCTTTGGAGCTTCATAACTCCTTCTTTGGAGCTGTTGATGAGCTGTTTCAGCTTCCAATTGAAACTCGAATCAAGAACACAAGTGAAAAGGCTTACCATGGCTACTTTGGCCGCTACTCCTTTCTTCCTCTCTATGAATCCATGGCTATTGATAACCCAACTCAGCTAGACGCTGCTCAAAGCTTTGCCAATCTCATGTGGCCGAATCAAGGAAACGATCGCTTCAGCGAAACTATTCAACGTTTCTCAACCTTGGTGGCGAAACTGGACAAGATAGTAACAAGAATGGTGCTGGAAAGCTACGGAGTATCAAAGCAGCTGATCGACACAAACGTAGATTCAACAGCCAACCTTCTCCGCTTCTTCAAATACAGAGTTCCTGAGCAACACGAAACCGATGTGGGACTGCACGCCCATACAGATCTCACTTTTTTCTCCATAATTCATCAACATCTCATCGGTGGTCTCCAAATTCAATCGCTCGATGATCAATGGATCGACCTCGAGCCCTCCAGCCACTGCTCCTTCATCGTCATGGCGGGTGACGCGCTCATGGCGTGGAGTAACGATAGAATAAGGAGTTGTAGGCATCGAGTGACGATGAAAGCGGAGGAAACGAGATACTCAGCGGGGATGTTTTCGTTCAAGAACGGAACGATTAGTGTGCCTGAAGAGCTTGTGGATGATGCCAACCCATTGCGATACAAGCCCTTTAATCACTATGACTTCCTTACTCATGACATAGCAAAGGGTTCTAAAAAATTTCTCTATCGTATTAGAGATTATTGCAGCATTTGA

Protein sequence

MGSETQARVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFFGAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPNQGNDRFSETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNVDSTANLLRFFKYRVPEQHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSNDRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDIAKGSKKFLYRIRDYCSI
Homology
BLAST of Csor.00g104350 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.3e-59
Identity = 123/294 (41.84%), Postives = 181/294 (61.56%), Query Frame = 0

Query: 8   RVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFFGAVDELF 67
           ++PVID S  + L+ GS  W      +   LE++GCF   F+++S+EL+ S F A+++LF
Sbjct: 6   QLPVIDFSD-QNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLF 65

Query: 68  QLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPNQGNDRFS 127
           +LPI T+ +N S K +HGY        LYES+ I++   L+    F   +WP+ GN   S
Sbjct: 66  ELPIPTKQRNVSSKLFHGYLCH----NLYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 125

Query: 128 ETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNVDSTANLLRFFKYRVP----EQHET 187
           ETI  FS  + +LD +V RM++ES+G+ K  ID +++ST  L R  KY  P    +  ET
Sbjct: 126 ETIHLFSEQLVELDLMVRRMIMESFGIEK-YIDEHLNSTYYLTRLMKYTSPPDDDDDEET 185

Query: 188 DVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSNDRI 247
            +GL +HTD    +I+HQ+ + GL++++ DD+WI ++PS   S +VM GD+L A  N R+
Sbjct: 186 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQD-SVLVMVGDSLCALLNGRL 245

Query: 248 RSCRHRVTMKAEETRYSAGMFSF-KNGT-ISVPEELVDDANPLRYKPFNHYDFL 296
            S  HRV M  ++TRYS G+FS  K G  I  PEELVD  +P  +KPF + DFL
Sbjct: 246 HSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFL 292

BLAST of Csor.00g104350 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 1.7e-59
Identity = 123/295 (41.69%), Postives = 181/295 (61.36%), Query Frame = 0

Query: 8   RVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFFGAVDELF 67
           ++PVID S  + L+ GS  W      +   LE++GCF   F+++S+EL+ S F A+++LF
Sbjct: 13  QLPVIDFSD-QNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDLF 72

Query: 68  QLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPNQGNDRFS 127
           +LPI T+ +N S K +HGY        LYES+ ID+   L+    F   +WP+ GN   S
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCH----NLYESLGIDDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 128 ETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNVDSTANLLRFFKYRVP-----EQHE 187
           ETI  FS  + +LD +V RM++ES+G+ +  ID +++ST  L R  KY  P     +  E
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGI-ENYIDEHLNSTYYLTRLMKYTSPPDDDDDDEE 192

Query: 188 TDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSNDR 247
           T +GL +HTD    +I+HQ+ + GL++++ DD+WI ++PS   S +VM GD+L A  N R
Sbjct: 193 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQD-SVLVMVGDSLCALLNGR 252

Query: 248 IRSCRHRVTMKAEETRYSAGMFSF-KNGT-ISVPEELVDDANPLRYKPFNHYDFL 296
           + S  HRV M  ++TRYS G+FS  K G  I  PEELVD  +P  +KPF + DFL
Sbjct: 253 LHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFL 300

BLAST of Csor.00g104350 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 6.6e-59
Identity = 122/296 (41.22%), Postives = 181/296 (61.15%), Query Frame = 0

Query: 8   RVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFFGAVDELF 67
           ++PVID S  + L+ GS  W      +   LE++GCF   F+++S+EL+ S F A+++LF
Sbjct: 13  QLPVIDFSD-QNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLF 72

Query: 68  QLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPNQGNDRFS 127
           +LPI T+ +N S K +HGY        LYES+ I++   L+    F   +WP+ GN   S
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCH----NLYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 128 ETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNVDSTANLLRFFKYRVP------EQH 187
           ETI  FS  + +LD +V RM++ES+G+ +  ID +++ST  L R  KY  P      +  
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGI-ENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDE 192

Query: 188 ETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSND 247
           ET +GL +HTD    +I+HQ+ + GL++++ DD+WI ++PS   S +VM GD+L A  N 
Sbjct: 193 ETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQD-SVLVMVGDSLCALLNG 252

Query: 248 RIRSCRHRVTMKAEETRYSAGMFSF-KNGT-ISVPEELVDDANPLRYKPFNHYDFL 296
           R+ S  HRV M  ++TRYS G+FS  K G  I  PEELVD  +P  +KPF + DFL
Sbjct: 253 RLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFL 301

BLAST of Csor.00g104350 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 1.6e-52
Identity = 115/313 (36.74%), Postives = 171/313 (54.63%), Query Frame = 0

Query: 1   MGSETQARVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60
           MGS    R+PVIDLS ++ L+ G+ +W S    +R  LEE+GCF  V + VS EL  +  
Sbjct: 1   MGSTAPLRLPVIDLS-MKNLKPGTTSWNSVRTQVREALEEYGCFEAVIDAVSPELQKAVC 60

Query: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPN 120
               EL  LP+ET++ N ++  Y G+    S   L E M +   T L+  + F NLMWP 
Sbjct: 61  NKGHELLNLPLETKMLNGNKPEYDGF---TSIPNLNEGMGVGRITDLEKVERFTNLMWP- 120

Query: 121 QGNDRFSETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNVDSTANLLRFFKYRVPEQ 180
           +GN  F ET+  +   +A++D I+  MV ES+G+ K   D+  +ST  LL F +Y+ P +
Sbjct: 121 EGNKDFCETVYSYGKRMAEVDHILKMMVFESFGMEKH-FDSFCESTNYLLHFMRYQQPGK 180

Query: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSN 240
                 L  H D +  +I++Q+ + GL+ ++ D +WI     +H   IV+ GD  MAWSN
Sbjct: 181 DGRSPALSLHKDKSILTIVNQNDVKGLEFETKDGEWILPTADNH---IVLLGDCFMAWSN 240

Query: 241 DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDIA 300
            R+ S  HRVT+ A + R S   FSF    I  P ELVD+ +PL + PF   + L +   
Sbjct: 241 GRLHSPLHRVTLVANQARLSTSSFSFPKDIIETPAELVDEEHPLLFNPFEITELLAYCFT 300

Query: 301 KGSKKFLYRIRDY 314
           K   K +  ++ Y
Sbjct: 301 KEGAKAVCDLKQY 304

BLAST of Csor.00g104350 vs. ExPASy Swiss-Prot
Match: Q9ZTA2 (Probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.0e-43
Identity = 121/403 (30.02%), Postives = 193/403 (47.89%), Query Frame = 0

Query: 1   MGSETQARVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60
           MGS    ++P+I+L+  + LE GS  W      +R  LE+FGCF   +++VSLEL  S  
Sbjct: 1   MGS-CSLQLPLINLAD-KTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIM 60

Query: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPN 120
             ++ELF LP+ET+ +N   K Y GY    +   L ES+ I N   L+    F   +WP+
Sbjct: 61  KTMEELFALPVETKQRNVCPKPYVGYLNHNN---LSESLGISNANILENINEFTQQLWPH 120

Query: 121 -QGNDRFSETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNVDSTA------------ 180
             GN+  S+TIQ F+  + ++D +V RMV+ES+G+ K  ID ++ STA            
Sbjct: 121 GDGNENISKTIQLFAEKLVEIDVMVRRMVMESFGIEK-YIDDHLKSTAYATDELNIVGVE 180

Query: 181 -------------------------------------NLLRFFKYRV------------- 240
                                                NL       V             
Sbjct: 181 PNVGVKVNADISDDVNANASVNAGVGANVNADTGVNDNLNVDANVAVGGGVNANTDLGVG 240

Query: 241 ---------------------PEQHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQW 300
                                 +  E  +GL  HTD   F+++ QH I GL++++ D++W
Sbjct: 241 VNVNSNVAVNAKTGGDDVEANDDNEEKKLGLPCHTDKNLFTVLFQHEIEGLEVKTKDEKW 300

Query: 301 IDLEPSSHCSFIVMAGDALMAWSNDRIRSCRHRVTM-KAEETRYSAGMFSF--KNGTISV 317
           I ++PS + +FIV+AGD+L A  N RIR+  HRV + + + TRY+A +F+    +  I  
Sbjct: 301 IRVKPSPN-TFIVIAGDSLCALMNGRIRAPYHRVRVTEKKRTRYTAAIFTCPKPDYVIEA 360

BLAST of Csor.00g104350 vs. NCBI nr
Match: KAG6575570.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1.2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 645 bits (1664), Expect = 1.09e-233
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0

Query: 1   MGSETQARVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60
           MGSETQARVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF
Sbjct: 1   MGSETQARVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60

Query: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPN 120
           GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPN
Sbjct: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPN 120

Query: 121 QGNDRFSETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNVDSTANLLRFFKYRVPEQ 180
           QGNDRFSETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNVDSTANLLRFFKYRVPEQ
Sbjct: 121 QGNDRFSETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNVDSTANLLRFFKYRVPEQ 180

Query: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSN 240
           HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSN
Sbjct: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSN 240

Query: 241 DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDIA 300
           DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDIA
Sbjct: 241 DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDIA 300

Query: 301 KGSKKFLYRIRDYCSI 316
           KGSKKFLYRIRDYCSI
Sbjct: 301 KGSKKFLYRIRDYCSI 316

BLAST of Csor.00g104350 vs. NCBI nr
Match: XP_023547382.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 638 bits (1645), Expect = 8.63e-231
Identity = 310/316 (98.10%), Postives = 315/316 (99.68%), Query Frame = 0

Query: 1   MGSETQARVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60
           MGSETQARVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLE+HNSFF
Sbjct: 1   MGSETQARVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLEIHNSFF 60

Query: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPN 120
           GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWP+
Sbjct: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPD 120

Query: 121 QGNDRFSETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNVDSTANLLRFFKYRVPEQ 180
           QGNDRFSETIQRFSTL+AKLDKIVTRMVLESYGVSKQLIDTN+DSTANLLRFFKYRVPEQ
Sbjct: 121 QGNDRFSETIQRFSTLMAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFFKYRVPEQ 180

Query: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSN 240
           HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSF+VMAGDALMAWSN
Sbjct: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFVVMAGDALMAWSN 240

Query: 241 DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDIA 300
           DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHD A
Sbjct: 241 DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDKA 300

Query: 301 KGSKKFLYRIRDYCSI 316
           KGSKKFLYRIRDYCSI
Sbjct: 301 KGSKKFLYRIRDYCSI 316

BLAST of Csor.00g104350 vs. NCBI nr
Match: XP_022953943.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata])

HSP 1 Score: 634 bits (1636), Expect = 2.03e-229
Identity = 310/316 (98.10%), Postives = 314/316 (99.37%), Query Frame = 0

Query: 1   MGSETQARVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60
           MGSETQA VPVIDLSKLEKLEAG+DAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF
Sbjct: 1   MGSETQAGVPVIDLSKLEKLEAGTDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60

Query: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPN 120
           GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWP+
Sbjct: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPD 120

Query: 121 QGNDRFSETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNVDSTANLLRFFKYRVPEQ 180
           QGN+RFSETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTN+DSTANLLRFFKYRVPEQ
Sbjct: 121 QGNNRFSETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFFKYRVPEQ 180

Query: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSN 240
           HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSN
Sbjct: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSN 240

Query: 241 DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDIA 300
           DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHD A
Sbjct: 241 DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDKA 300

Query: 301 KGSKKFLYRIRDYCSI 316
           KGSKKFLYRIRDYCSI
Sbjct: 301 KGSKKFLYRIRDYCSI 316

BLAST of Csor.00g104350 vs. NCBI nr
Match: XP_022992484.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita maxima])

HSP 1 Score: 619 bits (1596), Expect = 2.55e-223
Identity = 299/316 (94.62%), Postives = 311/316 (98.42%), Query Frame = 0

Query: 1   MGSETQARVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60
           MGSETQARVPVIDLSKLE LEAG++AWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF
Sbjct: 1   MGSETQARVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60

Query: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPN 120
           GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAI+NPTQLDAAQSFANLMWP 
Sbjct: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPA 120

Query: 121 QGNDRFSETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNVDSTANLLRFFKYRVPEQ 180
           QGN+ FSETIQRFSTL+AKLDKI+TRMVLESYGV+KQLIDTN+DSTANLLRFFKYRVPE+
Sbjct: 121 QGNNHFSETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVPEE 180

Query: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSN 240
           HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPS HCSF+VMAGDALMAWSN
Sbjct: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSFVVMAGDALMAWSN 240

Query: 241 DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDIA 300
           DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNH+DFLT+D A
Sbjct: 241 DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHFDFLTYDKA 300

Query: 301 KGSKKFLYRIRDYCSI 316
           KGSKKFLYRIRDYCSI
Sbjct: 301 KGSKKFLYRIRDYCSI 316

BLAST of Csor.00g104350 vs. NCBI nr
Match: KAG7014111.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1.2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 564 bits (1454), Expect = 6.83e-198
Identity = 279/304 (91.78%), Postives = 280/304 (92.11%), Query Frame = 0

Query: 13  DLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFFGAVDELFQLPIE 72
           DLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFFGAVDELFQLPIE
Sbjct: 281 DLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFFGAVDELFQLPIE 340

Query: 73  TRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPNQGNDRFSETIQR 132
           TRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWP+QGNDRFS     
Sbjct: 341 TRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPDQGNDRFS----- 400

Query: 133 FSTLVAKLDKIVTRMVLESYGVSKQLIDTNVDSTANLLRFFKYRVPEQHETDVGLHAHTD 192
                              YGVSKQLIDTNVDSTANLLRFFKYRVPEQHETDVGLHAHTD
Sbjct: 401 -------------------YGVSKQLIDTNVDSTANLLRFFKYRVPEQHETDVGLHAHTD 460

Query: 193 LTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSNDRIRSCRHRVTM 252
           LTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSNDRIRSCRHRVTM
Sbjct: 461 LTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSNDRIRSCRHRVTM 520

Query: 253 KAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDIAKGSKKFLYRIRD 312
           KAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDIAKGSKKFLYRIRD
Sbjct: 521 KAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDIAKGSKKFLYRIRD 560

Query: 313 YCSI 316
           YCSI
Sbjct: 581 YCSI 560

BLAST of Csor.00g104350 vs. ExPASy TrEMBL
Match: A0A6J1GPR2 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111456352 PE=3 SV=1)

HSP 1 Score: 634 bits (1636), Expect = 9.84e-230
Identity = 310/316 (98.10%), Postives = 314/316 (99.37%), Query Frame = 0

Query: 1   MGSETQARVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60
           MGSETQA VPVIDLSKLEKLEAG+DAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF
Sbjct: 1   MGSETQAGVPVIDLSKLEKLEAGTDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60

Query: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPN 120
           GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWP+
Sbjct: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPD 120

Query: 121 QGNDRFSETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNVDSTANLLRFFKYRVPEQ 180
           QGN+RFSETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTN+DSTANLLRFFKYRVPEQ
Sbjct: 121 QGNNRFSETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFFKYRVPEQ 180

Query: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSN 240
           HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSN
Sbjct: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSN 240

Query: 241 DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDIA 300
           DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHD A
Sbjct: 241 DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDKA 300

Query: 301 KGSKKFLYRIRDYCSI 316
           KGSKKFLYRIRDYCSI
Sbjct: 301 KGSKKFLYRIRDYCSI 316

BLAST of Csor.00g104350 vs. ExPASy TrEMBL
Match: A0A6J1JXN6 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 GN=LOC111488804 PE=3 SV=1)

HSP 1 Score: 619 bits (1596), Expect = 1.23e-223
Identity = 299/316 (94.62%), Postives = 311/316 (98.42%), Query Frame = 0

Query: 1   MGSETQARVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60
           MGSETQARVPVIDLSKLE LEAG++AWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF
Sbjct: 1   MGSETQARVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60

Query: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPN 120
           GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAI+NPTQLDAAQSFANLMWP 
Sbjct: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPA 120

Query: 121 QGNDRFSETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNVDSTANLLRFFKYRVPEQ 180
           QGN+ FSETIQRFSTL+AKLDKI+TRMVLESYGV+KQLIDTN+DSTANLLRFFKYRVPE+
Sbjct: 121 QGNNHFSETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVPEE 180

Query: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSN 240
           HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPS HCSF+VMAGDALMAWSN
Sbjct: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSFVVMAGDALMAWSN 240

Query: 241 DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDIA 300
           DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNH+DFLT+D A
Sbjct: 241 DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHFDFLTYDKA 300

Query: 301 KGSKKFLYRIRDYCSI 316
           KGSKKFLYRIRDYCSI
Sbjct: 301 KGSKKFLYRIRDYCSI 316

BLAST of Csor.00g104350 vs. ExPASy TrEMBL
Match: A0A1S3CDV2 (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499759 PE=3 SV=1)

HSP 1 Score: 462 bits (1189), Expect = 1.27e-161
Identity = 226/315 (71.75%), Postives = 259/315 (82.22%), Query Frame = 0

Query: 3   SETQARVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFFGA 62
           ++++  +P IDLSKLE    G+  WG  CK MR GLEEFGCF VV+++VSLELHNS   A
Sbjct: 5   AKSKVVIPTIDLSKLE---VGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVEA 64

Query: 63  VDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPNQG 122
             ELF LPIETR+KNTSEK YHGYFG YSFLPLYESMAIDNP QL + QSFANLMWP + 
Sbjct: 65  TKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRD 124

Query: 123 NDRFSETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNVDSTANLLRFFKYRVPEQHE 182
           N+ F  T++RFSTLVAKL+K+VT+MVLESYGVSK + +  ++ST  LLR FKYRV E+ E
Sbjct: 125 NNHFCVTVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDE 184

Query: 183 TDVGLHAHTDLTFFSIIHQHLIGGLQIQSL-DDQWIDLEPSSHCSFIVMAGDALMAWSND 242
           TDVGLH+HTDLTFFSIIHQHLI GLQIQSL DDQW+D+ PSSHCSFIVMAGDALMAWSN+
Sbjct: 185 TDVGLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNE 244

Query: 243 RIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDIAK 302
           RIRSCRHRV M+ EETRYS GMFSFKNGTI VP+  VDDANPLRYKPFNHYDFLT+D A 
Sbjct: 245 RIRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKAN 304

Query: 303 GSKKFLYRIRDYCSI 316
            S   + RI+DYC +
Sbjct: 305 ASHNTISRIKDYCGL 316

BLAST of Csor.00g104350 vs. ExPASy TrEMBL
Match: A0A6J1CXE5 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Momordica charantia OX=3673 GN=LOC111015077 PE=3 SV=1)

HSP 1 Score: 444 bits (1141), Expect = 2.66e-154
Identity = 215/320 (67.19%), Postives = 263/320 (82.19%), Query Frame = 0

Query: 1   MGSETQARVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60
           MG++ ++++P I+LS+ EKL+ GS+ W    K +RV LEEFGCF VV+E+VSLELHNS F
Sbjct: 1   MGTQAKSKIPTIELSE-EKLKVGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSIF 60

Query: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPN 120
            A  ELF LPIETRI+NTSEK YHGYFGRYSFLPLYESMA+DNPT L A Q+F NLMWP 
Sbjct: 61  EATKELFHLPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWP- 120

Query: 121 QGNDRFSETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNVDSTANLLRFFKYRVPEQ 180
            GN  F ET+  FSTLVAKL++ VTRMVLESYGVSKQ+ +++++ST +LLR FKYRVP+Q
Sbjct: 121 AGNKTFCETVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPKQ 180

Query: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDD----QWIDLEPSSHCSFIVMAGDALM 240
            ETD+GLH+HTDLTF SI+HQHLIGGLQ++SLD+    +WID++P    SFIVMAGDALM
Sbjct: 181 DETDLGLHSHTDLTFLSILHQHLIGGLQVKSLDNNNDGEWIDIDPVGRSSFIVMAGDALM 240

Query: 241 AWSNDRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLT 300
           AWSNDRIR CRHRV M+ +ETRYS G+FSFK+GTISVPEELV+D +PL+YKPF+H++FL 
Sbjct: 241 AWSNDRIRGCRHRVMMRGDETRYSLGLFSFKSGTISVPEELVEDNHPLKYKPFDHFEFLR 300

Query: 301 HDIAKGSKKFLYRIRDYCSI 316
           +D A  S K   RI+DYC I
Sbjct: 301 YDKANASHKTCSRIKDYCGI 318

BLAST of Csor.00g104350 vs. ExPASy TrEMBL
Match: A0A5A7USF0 (Putative 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G002400 PE=3 SV=1)

HSP 1 Score: 379 bits (972), Expect = 9.76e-130
Identity = 182/243 (74.90%), Postives = 207/243 (85.19%), Query Frame = 0

Query: 75  IKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPNQGNDRFSETIQRFS 134
           +KNTSEK YHGYFG YSFLPLYESMAIDNP QL + QSFANLMWP + N+ F  T++RFS
Sbjct: 1   MKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRDNNHFCVTVERFS 60

Query: 135 TLVAKLDKIVTRMVLESYGVSKQLIDTNVDSTANLLRFFKYRVPEQHETDVGLHAHTDLT 194
           TLVAKL+K+VT+MVLESYGVSK + +  ++ST  LLR FKYRV E+ ETDVGLH+HTDLT
Sbjct: 61  TLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDETDVGLHSHTDLT 120

Query: 195 FFSIIHQHLIGGLQIQSL-DDQWIDLEPSSHCSFIVMAGDALMAWSNDRIRSCRHRVTMK 254
           FFSIIHQHLI GLQIQSL DDQW+D+ PSSHCSFIVMAGDALMAWSN+RIRSCRH+V M+
Sbjct: 121 FFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNERIRSCRHKVIMR 180

Query: 255 AEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDIAKGSKKFLYRIRDY 314
            EETRYS GMFSFKNGTI VP+  VDDANPLRYKPFNHYDFLT+D A  S   + RI+DY
Sbjct: 181 GEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKANASHNTISRIKDY 240

Query: 315 CSI 316
           C +
Sbjct: 241 CGL 243

BLAST of Csor.00g104350 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 293.1 bits (749), Expect = 2.6e-79
Identity = 138/309 (44.66%), Postives = 200/309 (64.72%), Query Frame = 0

Query: 8   RVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFFGAVDELF 67
           +VP++DL+  ++L+  +  W S  +     LEE+GCF  V++ V+ +L +S F A +ELF
Sbjct: 9   KVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAEELF 68

Query: 68  QLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPNQGNDRFS 127
            LP ET+ KN +EK YHGY G+   +PL+E + +D  T  + AQ F +LMWP QGNDRF 
Sbjct: 69  DLPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWP-QGNDRFC 128

Query: 128 ETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNVDSTANLLRFFKYRVPEQHETDVGL 187
            T+  FS  VA+LD++V RM+ E+YGV K   +++V S   LL+F KY  P +  +    
Sbjct: 129 NTVHTFSNAVAELDRLVVRMIFENYGVEKH-YESHVGSKTYLLKFLKYLAPPESISMPAF 188

Query: 188 HAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSNDRIRSCR 247
             HTD TF SI+HQ+ + GL+++S D +WI L+     S++VMAGD  M WSNDRIRSC 
Sbjct: 189 PQHTDKTFLSILHQNDVNGLEVKSKDGEWISLQLPPK-SYVVMAGDISMGWSNDRIRSCE 248

Query: 248 HRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDIAKGSKKFL 307
           HRVTM+ ++TRY+ G+FSF    +S+PEELVDD +PL YKPF++   +     K  ++  
Sbjct: 249 HRVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEGREAN 308

Query: 308 YRIRDYCSI 317
             ++ YC I
Sbjct: 309 STLKAYCGI 314

BLAST of Csor.00g104350 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 268.9 bits (686), Expect = 5.3e-72
Identity = 135/308 (43.83%), Postives = 198/308 (64.29%), Query Frame = 0

Query: 8   RVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCV-VFEEVSLELHNSFFGAVDEL 67
           ++P +D S+ E L+ G+  W ST + +R  LEE+GCF + + ++  L+L +  FG++ +L
Sbjct: 4   KIPTLDFSR-EDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLVDL 63

Query: 68  FQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPNQGNDRF 127
           F LP +T++KN  +K  +GY G+   LPL+ES+ IDN T L+A +SF  LMWP QGN+ F
Sbjct: 64  FDLPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWP-QGNEHF 123

Query: 128 SETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNVDSTANLLRFFKYRVPEQHETDVG 187
           SE + +++   A+LD++VTRMV +SY V K   D  ++ST  LLR  K R P      +G
Sbjct: 124 SECLYKYAEFAAELDQMVTRMVFQSYNVEK-YYDPYIESTTYLLRVLKNRAPNNENPTLG 183

Query: 188 LHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSNDRIRSC 247
              HTD +F +I+HQ  + GL++++ + + I++  SS   F+V+AGDALMAWSNDR+ S 
Sbjct: 184 FVTHTDKSFTTILHQDQVNGLEMETREGERININLSSPSLFMVVAGDALMAWSNDRVWSP 243

Query: 248 RHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDIAKGSKKF 307
           RH+V +  E  RYS GMF+F NGT+ VPEEL+D  +PL YKPF+H   L         K 
Sbjct: 244 RHQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTDTGYKS 303

Query: 308 LYRIRDYC 315
              I+ YC
Sbjct: 304 ECPIKAYC 308

BLAST of Csor.00g104350 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 260.8 bits (665), Expect = 1.5e-69
Identity = 135/321 (42.06%), Postives = 199/321 (61.99%), Query Frame = 0

Query: 1   MGSET---QARVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHN 60
           MGSET     R+PVID S  + L+ G   W  T   ++  L+++G F   F+ +  EL  
Sbjct: 1   MGSETPLLPLRLPVIDFSN-KNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRK 60

Query: 61  SFFGAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLM 120
           S FGA++ELF LP++T+++N S+K +HGY G+Y  +PLYESM ID+    +   +F   +
Sbjct: 61  SVFGALEELFDLPLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKL 120

Query: 121 WPNQGNDRFSETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNVDSTANLLRFFKYRV 180
           WP QGN  FS TIQ FS  +++LD  + RM++ES+G+ K  ID ++ ST  LLR  KY+ 
Sbjct: 121 WP-QGNISFSTTIQSFSKKLSELDITIRRMIMESFGLDK-YIDEHLHSTNYLLRVMKYKG 180

Query: 181 PEQHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMA 240
           P+  ET VGL+AHTD    +I++Q+ + GL++Q+ D  WI ++P+   SF VM GD+L A
Sbjct: 181 PDTEETKVGLNAHTDKNIVTILYQNHVEGLEVQTKDKNWIKVKPTQD-SFTVMIGDSLYA 240

Query: 241 WSNDRIRSCRHRVTMKAEETRYSAGMFSFKNG--TISVPEELVDDANPLRYKPFNHYDFL 300
             N R+ S  HRV M   ETRYS G+FS       +S P+ELVD+ +P  +KPF+H +FL
Sbjct: 241 LLNGRLHSPYHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFL 300

Query: 301 THDIAKGSKKFLYRIRDYCSI 317
                +  ++    ++ YC I
Sbjct: 301 QFYYTEAGQRSQSALKTYCGI 317

BLAST of Csor.00g104350 vs. TAIR 10
Match: AT1G80320.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 243.8 bits (621), Expect = 1.8e-64
Identity = 129/324 (39.81%), Postives = 192/324 (59.26%), Query Frame = 0

Query: 1   MGSETQARVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60
           M S  +  +P++D S  +KL  G+  W +T   +R  +E  G F   F  VS +L ++  
Sbjct: 1   MDSHEEQCLPILDFSS-DKLVRGTSHWITTRDDVRRAMEGQGWFVAEFSGVSSDLRDNLL 60

Query: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGR-YSFLPLYESMAIDNPTQLDAAQSFANLMWP 120
             + E++ LP + +IKN + KA HGY         ++ES+ ID  T+L A + F+ L+WP
Sbjct: 61  AGMKEMYYLPDQIKIKNENHKASHGYMSMVVDDYRIHESLGIDYATELQACKDFSKLLWP 120

Query: 121 NQGNDRFSETIQRFSTLVAKLDKIVTRMVLESYGV-SKQLIDTNVDSTANLLRFFKYRVP 180
            QGND F +T   ++  +A+LD+ V RM+ ESYG+  K+   ++ +ST  LLR   YR  
Sbjct: 121 -QGNDPFCQTTHMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRMLSYRRQ 180

Query: 181 EQHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAW 240
           +  E + G  +HTD +F SI+HQ+ +GGLQ++++  QW+   PS    F+V++G  L AW
Sbjct: 181 QNGEANTGFVSHTDKSFMSILHQNHVGGLQLKTMTGQWVGFNPSP-TRFVVLSGMGLTAW 240

Query: 241 SNDRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNH------Y 300
           SNDRI++C H+V M A+E RYS G FSF  GTI  PEELVDD +PLRY PF H      Y
Sbjct: 241 SNDRIKACYHKVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHDGLLRFY 300

Query: 301 DFLTHDIAKGSKKFLYRIRDYCSI 317
           +   + + K S+  L   + YC +
Sbjct: 301 ESYINSLKKSSEDLL---QIYCGV 318

BLAST of Csor.00g104350 vs. TAIR 10
Match: AT4G03070.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 229.2 bits (583), Expect = 4.7e-60
Identity = 122/296 (41.22%), Postives = 181/296 (61.15%), Query Frame = 0

Query: 8   RVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFFGAVDELF 67
           ++PVID S  + L+ GS  W      +   LE++GCF   F+++S+EL+ S F A+++LF
Sbjct: 13  QLPVIDFSD-QNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLF 72

Query: 68  QLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPNQGNDRFS 127
           +LPI T+ +N S K +HGY        LYES+ I++   L+    F   +WP+ GN   S
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCH----NLYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 128 ETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNVDSTANLLRFFKYRVP------EQH 187
           ETI  FS  + +LD +V RM++ES+G+ +  ID +++ST  L R  KY  P      +  
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGI-ENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDE 192

Query: 188 ETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSND 247
           ET +GL +HTD    +I+HQ+ + GL++++ DD+WI ++PS   S +VM GD+L A  N 
Sbjct: 193 ETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQD-SVLVMVGDSLCALLNG 252

Query: 248 RIRSCRHRVTMKAEETRYSAGMFSF-KNGT-ISVPEELVDDANPLRYKPFNHYDFL 296
           R+ S  HRV M  ++TRYS G+FS  K G  I  PEELVD  +P  +KPF + DFL
Sbjct: 253 RLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFL 301

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q944Z51.3e-5941.84Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha... [more]
Q945B61.7e-5941.69Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=... [more]
Q9ZTA36.6e-5941.22Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37... [more]
A0A0N9HQ361.6e-5236.74Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=... [more]
Q9ZTA21.0e-4330.02Probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thali... [more]
Match NameE-valueIdentityDescription
KAG6575570.11.09e-233100.00putative 2-oxoglutarate-dependent dioxygenase AOP1.2, partial [Cucurbita argyros... [more]
XP_023547382.18.63e-23198.10probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita pepo subsp. pepo][more]
XP_022953943.12.03e-22998.10probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata][more]
XP_022992484.12.55e-22394.62probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita maxima][more]
KAG7014111.16.83e-19891.78putative 2-oxoglutarate-dependent dioxygenase AOP1.2, partial [Cucurbita argyros... [more]
Match NameE-valueIdentityDescription
A0A6J1GPR29.84e-23098.10probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662... [more]
A0A6J1JXN61.23e-22394.62probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 G... [more]
A0A1S3CDV21.27e-16171.75probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo OX... [more]
A0A6J1CXE52.66e-15467.19probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Momordica charantia OX=367... [more]
A0A5A7USF09.76e-13074.90Putative 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo va... [more]
Match NameE-valueIdentityDescription
AT1G52800.12.6e-7944.662-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G52790.15.3e-7243.832-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G52820.11.5e-6942.062-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G80320.11.8e-6439.812-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G03070.14.7e-6041.222-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 175..265
e-value: 3.0E-17
score: 62.9
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 9..88
e-value: 3.0E-10
score: 40.9
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 1..306
e-value: 4.2E-59
score: 202.4
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 4..315
NoneNo IPR availablePANTHERPTHR47990:SF1692-OXOGLUTARATE-DEPENDENT DIOXYGENASEcoord: 4..315
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 7..297
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 166..269
score: 11.104969

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g104350.m01Csor.00g104350.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding