Csor.00g103010 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g103010
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionMitochondrial substrate carrier family protein
LocationCsor_Chr20: 1681155 .. 1684588 (-)
RNA-Seq ExpressionCsor.00g103010
SyntenyCsor.00g103010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintronterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCACCGAGGCGACCGAAGCCTCGGCTAAGTTGAAGAGTCCGGATTTTCGCCCGGTACCGTCGCCTCCAGATTTCCATCCCGAGATTCTGGTCTCAGCTCATGATGGTCTTCGGTTCTGGCAGTTCATGATTGCTGGTTCAATCGCTGGGTCTGTTGAGCATATGGCTATGTTTCCGGTTGATACAGTCAAGACCCATATGCAAGCCCTTGGCTCTTGTCCAATTAAATCTGTTGGAGTTCGACAAGCGCTTCGGTCAATTTTGAAGACAGAGGGACCTGCTGGTTTTTATCGTGGTATTGGTGCTATGGGTCTTGGAGCTGGACCTGCACATGCTGTGTATTTTACAGTCTACGAGGATTGTAAGAAGTTTTTCTCCGGTGGGGATCCTAATAATTCTGTTGCCCACGCTGCTTCTGGCGTCTGTGCAACGGTGGCGAGTGACGCTGTTTTTACACCGATGGATATGGTTAAACAGAGGCTGCAGCTGAGTAATAGTCCCTACAAGGGGGTTTTGGATTGTGTCACAAGGGTCCTCAGAGACGAGGGGTTTAAAGCATTTTACGCATCATATAGAACTACAGTGTTAATGAATGCTCCATTTACTGCTGTGCATTTTGCTACTTATGAAGCTGCAAAGAGAGGTTTGCTGGAGGTTTCGCCAGAGATTGTAAATAATGAACAGTGGATCGTTCATGCGACGGCAGGAGCTGCTGCTGGAGCCTCAGCTGCATTTGTTACAACGCCGCTTGATGTGGTTAAGACTCAACTACAATGTCAGGTACCCCATTTAATAGTTTGGTCGTAGACTCTGCTAATTTTGTCCAGATTCCCCTTTTCCCATTTGCCGGAAGATTTTAACCATAAGTGTAGATCCCCATGGTTGGGGAGGAAAACGAAGCATAATTTATACGGGTGTGAGAACCTCCCCTAGCAGTGTTTATACGGGTGTGAGAACCTCCCCTAGCAGTGTTTATAGGGAAAGCCTAAAGAGACAATATCTACTAGCGGTGAGCTTAAGCTATTACAAATAGCATCAAAGTCAGACACGGGACGATGTGCCAGCAAGGAGCTTAAGTCCCGAAGGGGGTGGACATGAGGCGGTGTGCCAGCAAGGCTGTTGAGTCCCGAAGGGGGTGGATTGGGGTCCCACATCGATTAGAGAAGGGAACGAGTGCTAGCATGGACGTTGGGCCTCGAAGGGGTGGATTGTGAGATCCACGTCGATTGGAGAGGAGAACAAAGTATTCTTTACAAGGATGATCTACTAGCGGTGAGCTTAAGCTATTACAAATAGCATCAAAGTCAGACACGGGACGATGTGCCAGCAAGGAGCTTAAGTCCCGAAGGGGGTGGACATGAGGCGGTGTGCCAGCAAGGCTGTTGGGTCCCGAAGAGGTGGATTGGGGTCCCACATCGATTAGAGAAGGGAACGAGTGCTAGCATGGACGTTGGGCCTCGAAGGGGTGGATTGTGAGATCCACGTCGATTGGAGAGGAGAACAAAGCATTCTTTACAAGGATGTGGAAACTTCTTCCTAGCATATGGATTTTAAAAATCTTGAGTAGAAGTCTGACAATATTTGTTAGCGGGAAGAGAATGTATTTTTAGAGGTAGATTAAGGTCATTTTTTGGGGTGCTTTTGTCATGCCAAAAGAACTTTCTACTTGAAAATTTAAATTTCCAAAGCACTTGTTGGGCCAAGGTTCTTTGAGTGATTGGCCGAAAAGCGATAAGTACTAGTACCTAGCATCAAAATGTTCTGTCATTAAGCATTTTTTTACCAGTGCTTATAATTAGAGACCCCTAAGTGGAGGCCTCATTGGCTGAGGCTCGCAATCTCTTAACACGTACCCAAGATTGCTGGTTCAAATCTGCAAGTGAACTTATTAAGGAAAAACTTTGGTGTCTCCAATGTCTGGACAAGGGGCAGGTGCGAATTTTGAAAAGAGAAGTGCTTGTAATTAGAATACTTCTGCTCTTATTCCGGAAGCTCTACCTAACAACATTCTAAAGCTAAGAATGGTTATGTTCTCAGGGGGCAAAGGCAAGAGAAGAAAAAACATGACGTAATTGCAACGAAGCATTCCTTATAAGAGTGTGGAAATCTCTCCCTAGAAGACGCGTTTTAGAAACATTGAGGAGAAGTCCGAAAGTAAAAGCCTAAAGAGGACAATATTTGCTAGCAGTGGCTTGGACTGTTACACCCTGAGACCCCAAAAGGAACGGGAAAATGCCAGAAAAAGTTCATAACTAAAAGTTAAAATTATATCAGCTAACATGGTTTGAAAGAATACCGTTGAAGGTCCAGAGAGTAACTGAGAGTTGATAATTGTTGACGGAGAATGGGGGAGGAAAATTATGCCATACCCTTATTCTTTAAACTCTTTTTCCAGAGTTCTCCTATTTATTCAATCATAATTATTTGAATATCAAATTAGTCTGAACAAGGACGGGTCAGGGATGGATAGCAGAATCCATTTCCGAACCCAATATCATCATCTTACTAGTCTATATAAATCTGCGAAGGTTTTGTTCACAGAAATTGACTGTGTACTATGTGTTCTGTGAATTAAACAACAATCATTATCTCAATTTGTTTAGTCCATACCTACCACCTTCTTCTGATGTTAAACATTCAAGAGTTTGTCAATTTTGTATATGTTAGTTTATCTTTTGATGGACCCTGCTTGGTTAGCTATCTCTCTCTTAGGAATCTTTCAGTTCACCTTTCTTATGGAACAAGTGGAGTCTTTTGATAGTCTGGTCTTCTCTAATCTCTATATCTAAGATGTTTTGATAACTAATGATGCCTACCCCCAAAAGGATTCCTTCTTTTCCTTGGAATATTATAGGATGTACCTGTCTACTTTGTTCGTCACGAAAAAATCGAAAAGTACTAAAAATATAGTGAACTCTGCTACAGGTTACATGTGAATTGAAAGGAGAACAGACGATATAGATTTTTTTAGTTTTTTAGTGATAAGAAATGTATGAACTGTATAATTTAAGTTCTTGTTATATTGATATGGCAGCACGATATGAATGCTTCATGAACATGTAAAGGATGCATGATCAACTGAAGTATGCATAAAAATGGAATGTGTTAAGGATTGTACGATGCTTATGGAGCAGAAGCAGGCTCAATTTCACATGCTAAATTACCTGCTACTAGCGCTAGATTCATAGCCTAACCTTAAATTCACATTTGTGGATCCCCAGGGTGTATGTGGCTGCGATAGATTCAAAAGCGGGTCGATAGAAGACGTCGTTAGGACAATACTCAAGAAGGACGGATATCGAGGGCTTATGAGGGGATGGGTTCCAAGGATGCTCTTTCACGCTCCGGCTGCTGCTATTTGCTGGTCGACATACGAAGCGATAAAATCTCTGTTCCAAGAGCTCAATGGCAGCAGTGACGAAATGACATGA

mRNA sequence

ATGGCCACCGAGGCGACCGAAGCCTCGGCTAAGTTGAAGAGTCCGGATTTTCGCCCGGTACCGTCGCCTCCAGATTTCCATCCCGAGATTCTGGTCTCAGCTCATGATGGTCTTCGGTTCTGGCAGTTCATGATTGCTGGTTCAATCGCTGGGTCTGTTGAGCATATGGCTATGTTTCCGGTTGATACAGTCAAGACCCATATGCAAGCCCTTGGCTCTTGTCCAATTAAATCTGTTGGAGTTCGACAAGCGCTTCGGTCAATTTTGAAGACAGAGGGACCTGCTGGTTTTTATCGTGGTATTGGTGCTATGGGTCTTGGAGCTGGACCTGCACATGCTGTGTATTTTACAGTCTACGAGGATTGTAAGAAGTTTTTCTCCGGTGGGGATCCTAATAATTCTGTTGCCCACGCTGCTTCTGGCGTCTGTGCAACGGTGGCGAGTGACGCTGTTTTTACACCGATGGATATGGTTAAACAGAGGCTGCAGCTGAGTAATAGTCCCTACAAGGGGGTTTTGGATTGTGTCACAAGGGTCCTCAGAGACGAGGGGTTTAAAGCATTTTACGCATCATATAGAACTACAGTGTTAATGAATGCTCCATTTACTGCTGTGCATTTTGCTACTTATGAAGCTGCAAAGAGAGGTTTGCTGGAGGTTTCGCCAGAGATTGTAAATAATGAACAGTGGATCGTTCATGCGACGGCAGGAGCTGCTGCTGGAGCCTCAGCTGCATTTGTTACAACGCCGCTTGATGTGGTTAAGACTCAACTACAATGTCAGGGTGTATGTGGCTGCGATAGATTCAAAAGCGGGTCGATAGAAGACGTCGTTAGGACAATACTCAAGAAGGACGGATATCGAGGGCTTATGAGGGGATGGGTTCCAAGGATGCTCTTTCACGCTCCGGCTGCTGCTATTTGCTGGTCGACATACGAAGCGATAAAATCTCTGTTCCAAGAGCTCAATGGCAGCAGTGACGAAATGACATGA

Coding sequence (CDS)

ATGGCCACCGAGGCGACCGAAGCCTCGGCTAAGTTGAAGAGTCCGGATTTTCGCCCGGTACCGTCGCCTCCAGATTTCCATCCCGAGATTCTGGTCTCAGCTCATGATGGTCTTCGGTTCTGGCAGTTCATGATTGCTGGTTCAATCGCTGGGTCTGTTGAGCATATGGCTATGTTTCCGGTTGATACAGTCAAGACCCATATGCAAGCCCTTGGCTCTTGTCCAATTAAATCTGTTGGAGTTCGACAAGCGCTTCGGTCAATTTTGAAGACAGAGGGACCTGCTGGTTTTTATCGTGGTATTGGTGCTATGGGTCTTGGAGCTGGACCTGCACATGCTGTGTATTTTACAGTCTACGAGGATTGTAAGAAGTTTTTCTCCGGTGGGGATCCTAATAATTCTGTTGCCCACGCTGCTTCTGGCGTCTGTGCAACGGTGGCGAGTGACGCTGTTTTTACACCGATGGATATGGTTAAACAGAGGCTGCAGCTGAGTAATAGTCCCTACAAGGGGGTTTTGGATTGTGTCACAAGGGTCCTCAGAGACGAGGGGTTTAAAGCATTTTACGCATCATATAGAACTACAGTGTTAATGAATGCTCCATTTACTGCTGTGCATTTTGCTACTTATGAAGCTGCAAAGAGAGGTTTGCTGGAGGTTTCGCCAGAGATTGTAAATAATGAACAGTGGATCGTTCATGCGACGGCAGGAGCTGCTGCTGGAGCCTCAGCTGCATTTGTTACAACGCCGCTTGATGTGGTTAAGACTCAACTACAATGTCAGGGTGTATGTGGCTGCGATAGATTCAAAAGCGGGTCGATAGAAGACGTCGTTAGGACAATACTCAAGAAGGACGGATATCGAGGGCTTATGAGGGGATGGGTTCCAAGGATGCTCTTTCACGCTCCGGCTGCTGCTATTTGCTGGTCGACATACGAAGCGATAAAATCTCTGTTCCAAGAGCTCAATGGCAGCAGTGACGAAATGACATGA

Protein sequence

MATEATEASAKLKSPDFRPVPSPPDFHPEILVSAHDGLRFWQFMIAGSIAGSVEHMAMFPVDTVKTHMQALGSCPIKSVGVRQALRSILKTEGPAGFYRGIGAMGLGAGPAHAVYFTVYEDCKKFFSGGDPNNSVAHAASGVCATVASDAVFTPMDMVKQRLQLSNSPYKGVLDCVTRVLRDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLLEVSPEIVNNEQWIVHATAGAAAGASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIEDVVRTILKKDGYRGLMRGWVPRMLFHAPAAAICWSTYEAIKSLFQELNGSSDEMT
Homology
BLAST of Csor.00g103010 vs. ExPASy Swiss-Prot
Match: Q55DY8 (Mitoferrin OS=Dictyostelium discoideum OX=44689 GN=mcfF PE=3 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 7.9e-55
Identity = 118/299 (39.46%), Postives = 178/299 (59.53%), Query Frame = 0

Query: 36  DGLRFWQFMIAGSIAGSVEHMAMFPVDTVKTHMQALGSCPIKSVGVRQALRSILKTEGPA 95
           +G  F+  +IAG+ AG  EH  M+P+DT+KTH+QA+    +++  + Q  + I++  G  
Sbjct: 12  EGGSFYVHLIAGAAAGFAEHCGMYPIDTIKTHIQAIKPGAMQTSSL-QITKHIIQQHGIT 71

Query: 96  GFYRGIGAMGLGAGPAHAVYFTVYEDCK-KFFSGGDPNNSVAHAASGVCATVASDAVFTP 155
           G +RG+ A+  GA P+HAV+F++YE  K KF    + ++ +    +G  AT+ S+AV +P
Sbjct: 72  GLFRGLTAVAAGAAPSHAVHFSIYELLKFKFIGSDEDHHPIKVGIAGAIATMTSEAVASP 131

Query: 156 MDMVKQRLQLSNSPYKGVLDCVTRVLRDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAK 215
           MD+VKQRLQL  + YKG+ DC  R+   EG + FY+ Y TT++MN P+  V+FA+YE+ K
Sbjct: 132 MDVVKQRLQLQITDYKGLTDCTKRIWVKEGIRGFYSGYTTTLVMNVPYNIVYFASYESLK 191

Query: 216 RGLL----EVSPEIVNNEQWIVHATAGAAAGASAAFVTTPLDVVKTQLQCQG-------V 275
           + +       +PE   + Q I H  AG  AG  AA  T P DVVKT+LQ Q        +
Sbjct: 192 KIIQPWFNNKNPE-ERSYQLIDHLVAGGGAGMLAAAFTNPFDVVKTRLQTQSDFIASSTI 251

Query: 276 CGCDRFKS-GSIEDVVRTILKKDGYRGLMRGWVPRMLFHAPAAAICWSTYEAIKSLFQE 322
                 K  G + D ++TI  ++G  G +RG  PRM+FH+ ++AI WS YE  K +  E
Sbjct: 252 NSAKSIKRYGGMMDAMKTIWIEEGMDGYLRGMKPRMVFHSMSSAIVWSVYEYFKFILGE 308

BLAST of Csor.00g103010 vs. ExPASy Swiss-Prot
Match: Q620A6 (Mitoferrin OS=Caenorhabditis briggsae OX=6238 GN=mfn-1 PE=3 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 2.3e-54
Identity = 125/295 (42.37%), Postives = 164/295 (55.59%), Query Frame = 0

Query: 44  MIAGSIAGSVEHMAMFPVDTVKTHMQALGSCPIKSVGVRQALRSILKTEGPAGFYRGIGA 103
           + AG++AG+VEH  MFP D+VKT MQ+L  C  K      +L SI+K EG     RG+ A
Sbjct: 21  LAAGALAGAVEHCVMFPFDSVKTRMQSLCPCETKCPTPVHSLMSIVKREGWLRPLRGVNA 80

Query: 104 MGLGAGPAHAVYFTVYEDCKKFFSGGDP--NNSVAHAASGVCATVASDAVFTPMDMVKQR 163
           +  G+ PAHA+YFTVYE  K F +G      +++A+ ASGV AT+  DAV  P ++VKQR
Sbjct: 81  VAAGSMPAHALYFTVYEKMKSFLTGNTAGHEHTLAYGASGVVATLIHDAVMNPAEVVKQR 140

Query: 164 LQLSNSPYKGVLDCVTRVLRDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLLEVS 223
           +Q++ SPY   L+C   V   EGF AFY SY T + MN PF A+HF  YE  ++ L   +
Sbjct: 141 MQMAYSPYGSSLECARCVYNREGFAAFYRSYTTQLAMNVPFQAIHFMGYEFWQQVL---N 200

Query: 224 PEIVNNEQWIVHATAGAAAGASAAFVTTPLDVVKTQLQCQGVCGCD----------RFKS 283
           PE   + +   H  AG  AG  AA VTTP+D VKT L  Q     D          R++ 
Sbjct: 201 PEHKYDPK--SHLIAGGLAGGLAAAVTTPMDCVKTVLNTQQAAEADPSNRRIFLKARYRY 260

Query: 284 GSIEDVVRTILKKDGYRGLMRGWVPRMLFHAPAAAICWSTYEAIKSLFQELNGSS 327
             I D VRTI  + G  G   G   R++F  PA A+ WS YE  K +     G S
Sbjct: 261 RGISDAVRTIYSQRGMAGFSCGLQARVIFQVPATALSWSVYELFKFMLSFEGGHS 310

BLAST of Csor.00g103010 vs. ExPASy Swiss-Prot
Match: Q9VAY3 (Mitoferrin OS=Drosophila melanogaster OX=7227 GN=mfrn PE=2 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 5.1e-54
Identity = 121/273 (44.32%), Postives = 162/273 (59.34%), Query Frame = 0

Query: 44  MIAGSIAGSVEHMAMFPVDTVKTHMQALGSCPIKSVGVRQALRSILKTEGPAGFYRGIGA 103
           M AG+IAG +EH+ M+P+D+VKT MQ+L S P K++ +   LR+++  EG     RG  A
Sbjct: 18  MTAGAIAGVLEHVVMYPLDSVKTRMQSL-SPPTKNMNIVSTLRTMITREGLLRPIRGASA 77

Query: 104 MGLGAGPAHAVYFTVYEDCKKFFSGGDPNNSVAHAASGVCATVASDAVFTPMDMVKQRLQ 163
           + LGAGPAH++YF  YE  K+  +      ++ +  SG  AT+  DA+ +P D++KQR+Q
Sbjct: 78  VVLGAGPAHSLYFAAYEMTKELTAKFTSVRNLNYVISGAVATLIHDAISSPTDVIKQRMQ 137

Query: 164 LSNSPYKGVLDCVTRVLRDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLLEVSPE 223
           + NSPY  V+ CV  + + EGFKAFY +Y T ++MN P+  +HF TYE       +    
Sbjct: 138 MYNSPYTSVVSCVRDIYKREGFKAFYRAYGTQLVMNLPYQTIHFTTYE-----FFQNKMN 197

Query: 224 IVNNEQWIVHATAGAAAGASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIEDVVRTILK 283
           +       VH  AGAAAGA AA VTTPLDV+KT L  Q   G  R   G IE   R I  
Sbjct: 198 LERKYNPPVHMAAGAAAGACAAAVTTPLDVIKTLLNTQET-GLTR---GMIE-ASRKIYH 257

Query: 284 KDGYRGLMRGWVPRMLFHAPAAAICWSTYEAIK 317
             G  G  RG   R+L+  PA AICWSTYE  K
Sbjct: 258 MAGPLGFFRGTTARVLYSMPATAICWSTYEFFK 279

BLAST of Csor.00g103010 vs. ExPASy Swiss-Prot
Match: Q23125 (Mitoferrin OS=Caenorhabditis elegans OX=6239 GN=mfn-1 PE=2 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 5.6e-53
Identity = 123/296 (41.55%), Postives = 165/296 (55.74%), Query Frame = 0

Query: 44  MIAGSIAGSVEHMAMFPVDTVKTHMQALGSCP-IKSVGVRQALRSILKTEGPAGFYRGIG 103
           + AG++AG+VEH  MFP D+VKT MQ+L  CP  K      +L SI+K EG     RG+ 
Sbjct: 21  LTAGALAGAVEHCVMFPFDSVKTRMQSLCPCPETKCPTPVHSLMSIVKREGWLRPLRGVN 80

Query: 104 AMGLGAGPAHAVYFTVYEDCKKFFSGGDP--NNSVAHAASGVCATVASDAVFTPMDMVKQ 163
           A+  G+ PAHA+YFTVYE  K + +G     +N++A+ ASGV AT+  DA+  P ++VKQ
Sbjct: 81  AVAAGSMPAHALYFTVYEKMKGYLTGNSAGHSNTLAYGASGVVATLIHDAIMNPAEVVKQ 140

Query: 164 RLQLSNSPYKGVLDCVTRVLRDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLLEV 223
           R+Q++ SPY   L+C   V   EG  AFY SY T + MN PF A+HF +YE  +  L   
Sbjct: 141 RMQMAFSPYGSSLECARCVYNREGVAAFYRSYTTQLAMNVPFQAIHFMSYEFWQHVL--- 200

Query: 224 SPEIVNNEQWIVHATAGAAAGASAAFVTTPLDVVKTQLQCQGVCGCD----------RFK 283
           +PE   + +   H  AG  AG  AA +TTP+D VKT L  Q     D          R++
Sbjct: 201 NPEHKYDPK--SHLIAGGLAGGLAAALTTPMDCVKTVLNTQQAAEADPANRRIFLQARYR 260

Query: 284 SGSIEDVVRTILKKDGYRGLMRGWVPRMLFHAPAAAICWSTYEAIKSLFQELNGSS 327
              I D VRTI  + G  G   G   R++F  PA A+ WS YE  K +     G S
Sbjct: 261 YRGISDAVRTIYSQRGLSGFSCGLQARVIFQVPATALSWSVYELFKFMLSFEGGHS 311

BLAST of Csor.00g103010 vs. ExPASy Swiss-Prot
Match: Q287T7 (Mitoferrin-1 OS=Danio rerio OX=7955 GN=slc25a37 PE=1 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 1.8e-51
Identity = 134/327 (40.98%), Postives = 176/327 (53.82%), Query Frame = 0

Query: 1   MATEATEASAKLKSPDFRPVPSPPDFHPEILVSAHDGLRFWQFMIAGSIAGSVEHMAMFP 60
           + T+A  AS ++      PV +  D+     + AH  L     M AG++AG +EH  M+P
Sbjct: 3   LRTDAVLASLEMS----EPVKNDEDYES---LPAHASL--GTHMTAGAVAGILEHTVMYP 62

Query: 61  VDTVKTHMQALGSCP-IKSVGVRQALRSILKTEGPAGFYRGIGAMGLGAGPAHAVYFTVY 120
           VD+VKT MQ+L   P  +   V  AL+ I++TEG     RG+    LGAGPAHA+YF  Y
Sbjct: 63  VDSVKTRMQSLQPDPKAQYRSVYGALKRIVRTEGLLRPLRGLNITVLGAGPAHALYFACY 122

Query: 121 EDCKKFFSGGDPNNSVAHAASGVC---ATVASDAVFTPMDMVKQRLQLSNSPYKGVLDCV 180
           E  K+  S    N   +H A+GV    ATV  DAV  P ++VKQR+Q+ NSPY+ + DCV
Sbjct: 123 ERIKRSLSDVIQNGGNSHIANGVAGSVATVLHDAVMNPAEVVKQRMQMYNSPYRSLYDCV 182

Query: 181 TRVLRDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGL---LEVSPEIVNNEQWIVH 240
             V R EG  AFY SY T + MN PF AVHF TYE  +       +  PE         H
Sbjct: 183 LMVSRKEGLAAFYRSYSTQLTMNIPFQAVHFITYEFMQEHFNPHRQYRPE--------TH 242

Query: 241 ATAGAAAGASAAFVTTPLDVVKTQLQCQ-GVCGCDRFKSGSIEDVV---RTILKKDGYRG 300
             +GAAAGA +A VTTPLDV KT L  Q  V       SG +  +V   RT+ +  G   
Sbjct: 243 IISGAAAGAVSAAVTTPLDVCKTLLNTQENVALSSAHVSGHLSGMVNALRTVYRLGGVPA 302

Query: 301 LMRGWVPRMLFHAPAAAICWSTYEAIK 317
             +G   R+++  P+ AI WS YE  K
Sbjct: 303 FFKGIQARVIYQMPSTAIAWSVYEFFK 312

BLAST of Csor.00g103010 vs. NCBI nr
Match: XP_022943642.1 (mitoferrin-like [Cucurbita moschata] >KAG6570636.1 Mitoferrin, partial [Cucurbita argyrosperma subsp. sororia] >KAG7010487.1 Mitoferrin [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 667 bits (1720), Expect = 9.47e-242
Identity = 330/330 (100.00%), Postives = 330/330 (100.00%), Query Frame = 0

Query: 1   MATEATEASAKLKSPDFRPVPSPPDFHPEILVSAHDGLRFWQFMIAGSIAGSVEHMAMFP 60
           MATEATEASAKLKSPDFRPVPSPPDFHPEILVSAHDGLRFWQFMIAGSIAGSVEHMAMFP
Sbjct: 1   MATEATEASAKLKSPDFRPVPSPPDFHPEILVSAHDGLRFWQFMIAGSIAGSVEHMAMFP 60

Query: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKTEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120
           VDTVKTHMQALGSCPIKSVGVRQALRSILKTEGPAGFYRGIGAMGLGAGPAHAVYFTVYE
Sbjct: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKTEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120

Query: 121 DCKKFFSGGDPNNSVAHAASGVCATVASDAVFTPMDMVKQRLQLSNSPYKGVLDCVTRVL 180
           DCKKFFSGGDPNNSVAHAASGVCATVASDAVFTPMDMVKQRLQLSNSPYKGVLDCVTRVL
Sbjct: 121 DCKKFFSGGDPNNSVAHAASGVCATVASDAVFTPMDMVKQRLQLSNSPYKGVLDCVTRVL 180

Query: 181 RDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLLEVSPEIVNNEQWIVHATAGAAA 240
           RDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLLEVSPEIVNNEQWIVHATAGAAA
Sbjct: 181 RDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLLEVSPEIVNNEQWIVHATAGAAA 240

Query: 241 GASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIEDVVRTILKKDGYRGLMRGWVPRMLF 300
           GASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIEDVVRTILKKDGYRGLMRGWVPRMLF
Sbjct: 241 GASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIEDVVRTILKKDGYRGLMRGWVPRMLF 300

Query: 301 HAPAAAICWSTYEAIKSLFQELNGSSDEMT 330
           HAPAAAICWSTYEAIKSLFQELNGSSDEMT
Sbjct: 301 HAPAAAICWSTYEAIKSLFQELNGSSDEMT 330

BLAST of Csor.00g103010 vs. NCBI nr
Match: XP_023512983.1 (mitoferrin isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 665 bits (1717), Expect = 2.71e-241
Identity = 329/330 (99.70%), Postives = 330/330 (100.00%), Query Frame = 0

Query: 1   MATEATEASAKLKSPDFRPVPSPPDFHPEILVSAHDGLRFWQFMIAGSIAGSVEHMAMFP 60
           MATEATEASAKLKSPDFRPVPSPPDFHPEILVSAHDGLRFWQFMIAGSIAGSVEHMAMFP
Sbjct: 1   MATEATEASAKLKSPDFRPVPSPPDFHPEILVSAHDGLRFWQFMIAGSIAGSVEHMAMFP 60

Query: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKTEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120
           VDTVKTHMQALGSCPIKSVGVRQALRSILKTEGPAGFYRGIGAMGLGAGPAHAVYFTVYE
Sbjct: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKTEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120

Query: 121 DCKKFFSGGDPNNSVAHAASGVCATVASDAVFTPMDMVKQRLQLSNSPYKGVLDCVTRVL 180
           DCKKFFSGGDPNNSVAHAASGVCATVASDAVFTPMDMVKQRLQLSNSPYKGVLDCVTRVL
Sbjct: 121 DCKKFFSGGDPNNSVAHAASGVCATVASDAVFTPMDMVKQRLQLSNSPYKGVLDCVTRVL 180

Query: 181 RDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLLEVSPEIVNNEQWIVHATAGAAA 240
           RDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLLEVSPEIVNNEQWIVHATAGAAA
Sbjct: 181 RDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLLEVSPEIVNNEQWIVHATAGAAA 240

Query: 241 GASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIEDVVRTILKKDGYRGLMRGWVPRMLF 300
           GASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIEDVVRTILKKDGYRGLMRGWVPRMLF
Sbjct: 241 GASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIEDVVRTILKKDGYRGLMRGWVPRMLF 300

Query: 301 HAPAAAICWSTYEAIKSLFQELNGSSDEMT 330
           HAPAAAICWSTYEAIKS+FQELNGSSDEMT
Sbjct: 301 HAPAAAICWSTYEAIKSVFQELNGSSDEMT 330

BLAST of Csor.00g103010 vs. NCBI nr
Match: XP_022986893.1 (mitoferrin [Cucurbita maxima])

HSP 1 Score: 664 bits (1714), Expect = 7.78e-241
Identity = 328/330 (99.39%), Postives = 330/330 (100.00%), Query Frame = 0

Query: 1   MATEATEASAKLKSPDFRPVPSPPDFHPEILVSAHDGLRFWQFMIAGSIAGSVEHMAMFP 60
           MATEATEASAKLKSPDFRPVPSPPDFHPEI+VSAHDGLRFWQFMIAGSIAGSVEHMAMFP
Sbjct: 1   MATEATEASAKLKSPDFRPVPSPPDFHPEIVVSAHDGLRFWQFMIAGSIAGSVEHMAMFP 60

Query: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKTEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120
           VDTVKTHMQALGSCPIKSVGVRQALRSILKTEGPAGFYRGIGAMGLGAGPAHAVYFTVYE
Sbjct: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKTEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120

Query: 121 DCKKFFSGGDPNNSVAHAASGVCATVASDAVFTPMDMVKQRLQLSNSPYKGVLDCVTRVL 180
           DCKKFFSGGDPNNSVAHAASGVCATVASDAVFTPMDMVKQRLQLSNSPYKGVLDCVTRVL
Sbjct: 121 DCKKFFSGGDPNNSVAHAASGVCATVASDAVFTPMDMVKQRLQLSNSPYKGVLDCVTRVL 180

Query: 181 RDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLLEVSPEIVNNEQWIVHATAGAAA 240
           RDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLLEVSP+IVNNEQWIVHATAGAAA
Sbjct: 181 RDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLLEVSPDIVNNEQWIVHATAGAAA 240

Query: 241 GASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIEDVVRTILKKDGYRGLMRGWVPRMLF 300
           GASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIEDVVRTILKKDGYRGLMRGWVPRMLF
Sbjct: 241 GASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIEDVVRTILKKDGYRGLMRGWVPRMLF 300

Query: 301 HAPAAAICWSTYEAIKSLFQELNGSSDEMT 330
           HAPAAAICWSTYEAIKSLFQELNGSSDEMT
Sbjct: 301 HAPAAAICWSTYEAIKSLFQELNGSSDEMT 330

BLAST of Csor.00g103010 vs. NCBI nr
Match: XP_022148452.1 (mitoferrin [Momordica charantia])

HSP 1 Score: 627 bits (1616), Expect = 6.76e-226
Identity = 302/330 (91.52%), Postives = 320/330 (96.97%), Query Frame = 0

Query: 1   MATEATEASAKLKSPDFRPVPSPPDFHPEILVSAHDGLRFWQFMIAGSIAGSVEHMAMFP 60
           MATEATEA+ K ++PDFRPVPSPPDFHPEI+VSAHDGLRFW+FM+AGS+AGSVEHMAMFP
Sbjct: 1   MATEATEATTKFQNPDFRPVPSPPDFHPEIVVSAHDGLRFWEFMVAGSVAGSVEHMAMFP 60

Query: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKTEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120
           VDTVKTHMQALGSCPIKSVGVRQALRSILK+EGPAGFYRGIGAMGLGAGPAHAVYFTVYE
Sbjct: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120

Query: 121 DCKKFFSGGDPNNSVAHAASGVCATVASDAVFTPMDMVKQRLQLSNSPYKGVLDCVTRVL 180
           +CKKFFSGGDPNNSVAHA SGVCATVASDAVFTPMDMVKQRLQLSNSPYKGVLDCV RVL
Sbjct: 121 NCKKFFSGGDPNNSVAHAVSGVCATVASDAVFTPMDMVKQRLQLSNSPYKGVLDCVKRVL 180

Query: 181 RDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLLEVSPEIVNNEQWIVHATAGAAA 240
           RDEG  AFYASYRTTVLMNAPFTAVHF+TYEAAKRGL+EVSPEIVN+EQWIVHATAGAAA
Sbjct: 181 RDEGLGAFYASYRTTVLMNAPFTAVHFSTYEAAKRGLMEVSPEIVNDEQWIVHATAGAAA 240

Query: 241 GASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIEDVVRTILKKDGYRGLMRGWVPRMLF 300
           GASAAFVTTPLDVVKTQLQCQG+CGCDRFKS SI DV+RTILKKDGYRGLMRGW+PRMLF
Sbjct: 241 GASAAFVTTPLDVVKTQLQCQGICGCDRFKSSSIGDVIRTILKKDGYRGLMRGWIPRMLF 300

Query: 301 HAPAAAICWSTYEAIKSLFQELNGSSDEMT 330
           HAPAAAICWSTYEA+KS FQE+NGSS E+T
Sbjct: 301 HAPAAAICWSTYEALKSFFQEVNGSSGEVT 330

BLAST of Csor.00g103010 vs. NCBI nr
Match: XP_004143170.1 (mitoferrin [Cucumis sativus] >KGN47047.1 hypothetical protein Csa_020661 [Cucumis sativus])

HSP 1 Score: 624 bits (1609), Expect = 8.18e-225
Identity = 300/326 (92.02%), Postives = 316/326 (96.93%), Query Frame = 0

Query: 1   MATEATEASAKLKSPDFRPVPSPPDFHPEILVSAHDGLRFWQFMIAGSIAGSVEHMAMFP 60
           MATEATEA+ K +SPDFRPVPSPPDFHPEI+VSAHDGLRFWQFMIAGSIAGS EHMAMFP
Sbjct: 1   MATEATEATTKFQSPDFRPVPSPPDFHPEIVVSAHDGLRFWQFMIAGSIAGSAEHMAMFP 60

Query: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKTEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120
           +DTVKTHMQALGSCPIKSVGVRQALRSILK+EGPAGFYRGIGAMGLGAGPAHAVYFTVYE
Sbjct: 61  IDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120

Query: 121 DCKKFFSGGDPNNSVAHAASGVCATVASDAVFTPMDMVKQRLQLSNSPYKGVLDCVTRVL 180
           +CKKFFSGGDPNNS+AHAASGVCATVASDAVFTPMDMVKQRLQLSN+PYKGVLDC+ +VL
Sbjct: 121 NCKKFFSGGDPNNSLAHAASGVCATVASDAVFTPMDMVKQRLQLSNNPYKGVLDCIKKVL 180

Query: 181 RDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLLEVSPEIVNNEQWIVHATAGAAA 240
           RDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGL+EVSPE VN+EQW+VHATAGA A
Sbjct: 181 RDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLMEVSPESVNDEQWVVHATAGAVA 240

Query: 241 GASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIEDVVRTILKKDGYRGLMRGWVPRMLF 300
           GASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSI DV+RTILKKDGYRGLMRGWVPRMLF
Sbjct: 241 GASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIRDVIRTILKKDGYRGLMRGWVPRMLF 300

Query: 301 HAPAAAICWSTYEAIKSLFQELNGSS 326
           HAPAAAICWSTYEA+KS F +LNG S
Sbjct: 301 HAPAAAICWSTYEALKSFFHDLNGGS 326

BLAST of Csor.00g103010 vs. ExPASy TrEMBL
Match: A0A6J1FTL2 (mitoferrin-like OS=Cucurbita moschata OX=3662 GN=LOC111448348 PE=3 SV=1)

HSP 1 Score: 667 bits (1720), Expect = 4.58e-242
Identity = 330/330 (100.00%), Postives = 330/330 (100.00%), Query Frame = 0

Query: 1   MATEATEASAKLKSPDFRPVPSPPDFHPEILVSAHDGLRFWQFMIAGSIAGSVEHMAMFP 60
           MATEATEASAKLKSPDFRPVPSPPDFHPEILVSAHDGLRFWQFMIAGSIAGSVEHMAMFP
Sbjct: 1   MATEATEASAKLKSPDFRPVPSPPDFHPEILVSAHDGLRFWQFMIAGSIAGSVEHMAMFP 60

Query: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKTEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120
           VDTVKTHMQALGSCPIKSVGVRQALRSILKTEGPAGFYRGIGAMGLGAGPAHAVYFTVYE
Sbjct: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKTEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120

Query: 121 DCKKFFSGGDPNNSVAHAASGVCATVASDAVFTPMDMVKQRLQLSNSPYKGVLDCVTRVL 180
           DCKKFFSGGDPNNSVAHAASGVCATVASDAVFTPMDMVKQRLQLSNSPYKGVLDCVTRVL
Sbjct: 121 DCKKFFSGGDPNNSVAHAASGVCATVASDAVFTPMDMVKQRLQLSNSPYKGVLDCVTRVL 180

Query: 181 RDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLLEVSPEIVNNEQWIVHATAGAAA 240
           RDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLLEVSPEIVNNEQWIVHATAGAAA
Sbjct: 181 RDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLLEVSPEIVNNEQWIVHATAGAAA 240

Query: 241 GASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIEDVVRTILKKDGYRGLMRGWVPRMLF 300
           GASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIEDVVRTILKKDGYRGLMRGWVPRMLF
Sbjct: 241 GASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIEDVVRTILKKDGYRGLMRGWVPRMLF 300

Query: 301 HAPAAAICWSTYEAIKSLFQELNGSSDEMT 330
           HAPAAAICWSTYEAIKSLFQELNGSSDEMT
Sbjct: 301 HAPAAAICWSTYEAIKSLFQELNGSSDEMT 330

BLAST of Csor.00g103010 vs. ExPASy TrEMBL
Match: A0A6J1JHV3 (mitoferrin OS=Cucurbita maxima OX=3661 GN=LOC111484495 PE=3 SV=1)

HSP 1 Score: 664 bits (1714), Expect = 3.77e-241
Identity = 328/330 (99.39%), Postives = 330/330 (100.00%), Query Frame = 0

Query: 1   MATEATEASAKLKSPDFRPVPSPPDFHPEILVSAHDGLRFWQFMIAGSIAGSVEHMAMFP 60
           MATEATEASAKLKSPDFRPVPSPPDFHPEI+VSAHDGLRFWQFMIAGSIAGSVEHMAMFP
Sbjct: 1   MATEATEASAKLKSPDFRPVPSPPDFHPEIVVSAHDGLRFWQFMIAGSIAGSVEHMAMFP 60

Query: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKTEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120
           VDTVKTHMQALGSCPIKSVGVRQALRSILKTEGPAGFYRGIGAMGLGAGPAHAVYFTVYE
Sbjct: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKTEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120

Query: 121 DCKKFFSGGDPNNSVAHAASGVCATVASDAVFTPMDMVKQRLQLSNSPYKGVLDCVTRVL 180
           DCKKFFSGGDPNNSVAHAASGVCATVASDAVFTPMDMVKQRLQLSNSPYKGVLDCVTRVL
Sbjct: 121 DCKKFFSGGDPNNSVAHAASGVCATVASDAVFTPMDMVKQRLQLSNSPYKGVLDCVTRVL 180

Query: 181 RDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLLEVSPEIVNNEQWIVHATAGAAA 240
           RDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLLEVSP+IVNNEQWIVHATAGAAA
Sbjct: 181 RDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLLEVSPDIVNNEQWIVHATAGAAA 240

Query: 241 GASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIEDVVRTILKKDGYRGLMRGWVPRMLF 300
           GASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIEDVVRTILKKDGYRGLMRGWVPRMLF
Sbjct: 241 GASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIEDVVRTILKKDGYRGLMRGWVPRMLF 300

Query: 301 HAPAAAICWSTYEAIKSLFQELNGSSDEMT 330
           HAPAAAICWSTYEAIKSLFQELNGSSDEMT
Sbjct: 301 HAPAAAICWSTYEAIKSLFQELNGSSDEMT 330

BLAST of Csor.00g103010 vs. ExPASy TrEMBL
Match: A0A6J1D537 (mitoferrin OS=Momordica charantia OX=3673 GN=LOC111017091 PE=3 SV=1)

HSP 1 Score: 627 bits (1616), Expect = 3.27e-226
Identity = 302/330 (91.52%), Postives = 320/330 (96.97%), Query Frame = 0

Query: 1   MATEATEASAKLKSPDFRPVPSPPDFHPEILVSAHDGLRFWQFMIAGSIAGSVEHMAMFP 60
           MATEATEA+ K ++PDFRPVPSPPDFHPEI+VSAHDGLRFW+FM+AGS+AGSVEHMAMFP
Sbjct: 1   MATEATEATTKFQNPDFRPVPSPPDFHPEIVVSAHDGLRFWEFMVAGSVAGSVEHMAMFP 60

Query: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKTEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120
           VDTVKTHMQALGSCPIKSVGVRQALRSILK+EGPAGFYRGIGAMGLGAGPAHAVYFTVYE
Sbjct: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120

Query: 121 DCKKFFSGGDPNNSVAHAASGVCATVASDAVFTPMDMVKQRLQLSNSPYKGVLDCVTRVL 180
           +CKKFFSGGDPNNSVAHA SGVCATVASDAVFTPMDMVKQRLQLSNSPYKGVLDCV RVL
Sbjct: 121 NCKKFFSGGDPNNSVAHAVSGVCATVASDAVFTPMDMVKQRLQLSNSPYKGVLDCVKRVL 180

Query: 181 RDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLLEVSPEIVNNEQWIVHATAGAAA 240
           RDEG  AFYASYRTTVLMNAPFTAVHF+TYEAAKRGL+EVSPEIVN+EQWIVHATAGAAA
Sbjct: 181 RDEGLGAFYASYRTTVLMNAPFTAVHFSTYEAAKRGLMEVSPEIVNDEQWIVHATAGAAA 240

Query: 241 GASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIEDVVRTILKKDGYRGLMRGWVPRMLF 300
           GASAAFVTTPLDVVKTQLQCQG+CGCDRFKS SI DV+RTILKKDGYRGLMRGW+PRMLF
Sbjct: 241 GASAAFVTTPLDVVKTQLQCQGICGCDRFKSSSIGDVIRTILKKDGYRGLMRGWIPRMLF 300

Query: 301 HAPAAAICWSTYEAIKSLFQELNGSSDEMT 330
           HAPAAAICWSTYEA+KS FQE+NGSS E+T
Sbjct: 301 HAPAAAICWSTYEALKSFFQEVNGSSGEVT 330

BLAST of Csor.00g103010 vs. ExPASy TrEMBL
Match: A0A0A0KF81 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G181520 PE=3 SV=1)

HSP 1 Score: 624 bits (1609), Expect = 3.96e-225
Identity = 300/326 (92.02%), Postives = 316/326 (96.93%), Query Frame = 0

Query: 1   MATEATEASAKLKSPDFRPVPSPPDFHPEILVSAHDGLRFWQFMIAGSIAGSVEHMAMFP 60
           MATEATEA+ K +SPDFRPVPSPPDFHPEI+VSAHDGLRFWQFMIAGSIAGS EHMAMFP
Sbjct: 1   MATEATEATTKFQSPDFRPVPSPPDFHPEIVVSAHDGLRFWQFMIAGSIAGSAEHMAMFP 60

Query: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKTEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120
           +DTVKTHMQALGSCPIKSVGVRQALRSILK+EGPAGFYRGIGAMGLGAGPAHAVYFTVYE
Sbjct: 61  IDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120

Query: 121 DCKKFFSGGDPNNSVAHAASGVCATVASDAVFTPMDMVKQRLQLSNSPYKGVLDCVTRVL 180
           +CKKFFSGGDPNNS+AHAASGVCATVASDAVFTPMDMVKQRLQLSN+PYKGVLDC+ +VL
Sbjct: 121 NCKKFFSGGDPNNSLAHAASGVCATVASDAVFTPMDMVKQRLQLSNNPYKGVLDCIKKVL 180

Query: 181 RDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLLEVSPEIVNNEQWIVHATAGAAA 240
           RDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGL+EVSPE VN+EQW+VHATAGA A
Sbjct: 181 RDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLMEVSPESVNDEQWVVHATAGAVA 240

Query: 241 GASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIEDVVRTILKKDGYRGLMRGWVPRMLF 300
           GASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSI DV+RTILKKDGYRGLMRGWVPRMLF
Sbjct: 241 GASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIRDVIRTILKKDGYRGLMRGWVPRMLF 300

Query: 301 HAPAAAICWSTYEAIKSLFQELNGSS 326
           HAPAAAICWSTYEA+KS F +LNG S
Sbjct: 301 HAPAAAICWSTYEALKSFFHDLNGGS 326

BLAST of Csor.00g103010 vs. ExPASy TrEMBL
Match: A0A1S3CK52 (mitoferrin-like OS=Cucumis melo OX=3656 GN=LOC103501881 PE=3 SV=1)

HSP 1 Score: 625 bits (1612), Expect = 4.71e-225
Identity = 301/328 (91.77%), Postives = 317/328 (96.65%), Query Frame = 0

Query: 1   MATEATEASAKLKSPDFRPVPSPPDFHPEILVSAHDGLRFWQFMIAGSIAGSVEHMAMFP 60
           MATEATEA+ K +SPDFRPVPSPPDFHPEI+VSAHDGLRFWQFMIAGSIAGS EHMAMFP
Sbjct: 34  MATEATEATTKFQSPDFRPVPSPPDFHPEIVVSAHDGLRFWQFMIAGSIAGSAEHMAMFP 93

Query: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKTEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120
           +DTVKTHMQALGSCPIKSVGVRQALRSILK+EGPAGFYRGIGAMGLGAGPAHAVYFTVYE
Sbjct: 94  IDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 153

Query: 121 DCKKFFSGGDPNNSVAHAASGVCATVASDAVFTPMDMVKQRLQLSNSPYKGVLDCVTRVL 180
           +CKKFFSGGDPNNS+AHAASGVCATVASDAVFTPMDMVKQRLQLSN+PYKGVLDC+ +VL
Sbjct: 154 NCKKFFSGGDPNNSLAHAASGVCATVASDAVFTPMDMVKQRLQLSNNPYKGVLDCIKKVL 213

Query: 181 RDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLLEVSPEIVNNEQWIVHATAGAAA 240
           RDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGL+EVSPE VN+EQWIVHATAGA A
Sbjct: 214 RDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLMEVSPESVNDEQWIVHATAGAVA 273

Query: 241 GASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIEDVVRTILKKDGYRGLMRGWVPRMLF 300
           GASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSI DV+RTILKKDGYRGLMRGWVPRMLF
Sbjct: 274 GASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIRDVIRTILKKDGYRGLMRGWVPRMLF 333

Query: 301 HAPAAAICWSTYEAIKSLFQELNGSSDE 328
           HAPAAAICWSTYEA+KS F +LNG S +
Sbjct: 334 HAPAAAICWSTYEALKSFFHDLNGGSSD 361

BLAST of Csor.00g103010 vs. TAIR 10
Match: AT2G30160.1 (Mitochondrial substrate carrier family protein )

HSP 1 Score: 506.1 bits (1302), Expect = 2.1e-143
Identity = 244/328 (74.39%), Postives = 273/328 (83.23%), Query Frame = 0

Query: 5   ATEASAKLKSPDFRPVPSPPDFHPEILVSAHD-GLRFWQFMIAGSIAGSVEHMAMFPVDT 64
           ATEA+ K    D RP+P PPDFHP I+V A +  L+FWQ M+AGSIAGSVEHMAMFPVDT
Sbjct: 2   ATEATTKFPESDLRPIPQPPDFHPAIIVPAQNTTLKFWQLMVAGSIAGSVEHMAMFPVDT 61

Query: 65  VKTHMQALGSCPIKSVGVRQALRSILKTEGPAGFYRGIGAMGLGAGPAHAVYFTVYEDCK 124
           VKTHMQAL SCPIK +G+RQA RSI+KT+GP+  YRGI AMGLGAGPAHAVYF+ YE  K
Sbjct: 62  VKTHMQALRSCPIKPIGIRQAFRSIIKTDGPSALYRGIWAMGLGAGPAHAVYFSFYEVSK 121

Query: 125 KFFSGGDPNNSVAHAASGVCATVASDAVFTPMDMVKQRLQLSNSPYKGVLDCVTRVLRDE 184
           KF SGG+PNNS AHA SGV AT++SDAVFTPMDMVKQRLQ+ N  YKGV DC+ RV R+E
Sbjct: 122 KFLSGGNPNNSAAHAISGVFATISSDAVFTPMDMVKQRLQIGNGTYKGVWDCIKRVTREE 181

Query: 185 GFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLLEVSPE----IVNNEQWIVHATAGAA 244
           GF AFYASYRTTVLMNAPFTAVHF TYEA KRGL E+ PE      + E W+++ATAGAA
Sbjct: 182 GFGAFYASYRTTVLMNAPFTAVHFTTYEAVKRGLREMLPEHAVGAEDEEGWLIYATAGAA 241

Query: 245 AGASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIEDVVRTILKKDGYRGLMRGWVPRML 304
           AG  AA VTTPLDVVKTQLQCQGVCGCDRFKS SI DV RTI+KKDGYRGL RGW+PRML
Sbjct: 242 AGGLAAAVTTPLDVVKTQLQCQGVCGCDRFKSSSISDVFRTIVKKDGYRGLARGWLPRML 301

Query: 305 FHAPAAAICWSTYEAIKSLFQELNGSSD 328
           FHAPAAAICWSTYE +KS FQ+LNG ++
Sbjct: 302 FHAPAAAICWSTYETVKSFFQDLNGEAN 329

BLAST of Csor.00g103010 vs. TAIR 10
Match: AT1G07030.1 (Mitochondrial substrate carrier family protein )

HSP 1 Score: 497.7 bits (1280), Expect = 7.4e-141
Identity = 244/328 (74.39%), Postives = 274/328 (83.54%), Query Frame = 0

Query: 1   MATEATEASAKLKSPDFRPVPSPPDFHPEILVSAHDGLRFWQFMIAGSIAGSVEHMAMFP 60
           MATEAT    K + PD R V   PDF PEI   AHDGL+FWQFMIAGSIAGSVEHMAMFP
Sbjct: 1   MATEATTV-PKFQEPDLRQVSQTPDFKPEI---AHDGLKFWQFMIAGSIAGSVEHMAMFP 60

Query: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKTEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120
           VDT+KTHMQAL  CP+K VG+R+A RSI++ EGP+  YRGI AMGLGAGPAHAVYF+ YE
Sbjct: 61  VDTIKTHMQALRPCPLKPVGIREAFRSIIQKEGPSALYRGIWAMGLGAGPAHAVYFSFYE 120

Query: 121 DCKKFFSGGDPNNSVAHAASGVCATVASDAVFTPMDMVKQRLQLSNSPYKGVLDCVTRVL 180
             KK+ S GD NNSVAHA SGV AT++SDAVFTPMDMVKQRLQ+    YKGV DCV RVL
Sbjct: 121 VSKKYLSAGDQNNSVAHAMSGVFATISSDAVFTPMDMVKQRLQMGEGTYKGVWDCVKRVL 180

Query: 181 RDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLLEVSPEIVNNEQ-WIVHATAGAA 240
           R+EG  AFYASYRTTVLMNAPFTAVHFATYEAAK+GL+E SP+ +++E+ W+VHATAGAA
Sbjct: 181 REEGIGAFYASYRTTVLMNAPFTAVHFATYEAAKKGLMEFSPDRISDEEGWLVHATAGAA 240

Query: 241 AGASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIEDVVRTILKKDGYRGLMRGWVPRML 300
           AG  AA VTTPLDVVKTQLQCQGVCGCDRF S SI  V+RTI+KKDGYRGL+RGW+PRML
Sbjct: 241 AGGLAAAVTTPLDVVKTQLQCQGVCGCDRFTSSSISHVLRTIVKKDGYRGLLRGWLPRML 300

Query: 301 FHAPAAAICWSTYEAIKSLFQELNGSSD 328
           FHAPAAAICWSTYE +KS FQ+ N  S+
Sbjct: 301 FHAPAAAICWSTYEGVKSFFQDFNVDSN 324

BLAST of Csor.00g103010 vs. TAIR 10
Match: AT1G74240.1 (Mitochondrial substrate carrier family protein )

HSP 1 Score: 147.1 bits (370), Expect = 2.4e-35
Identity = 101/349 (28.94%), Postives = 162/349 (46.42%), Query Frame = 0

Query: 13  KSPDFRPVPSPPDFHPEILVSA-HDGLRFWQFMIAGSIAGSVEHMAMFPVDTVKTHMQA- 72
           KS D     SPP F   + + A HD    W+  + G IAG+     M PVDT+KT +Q+ 
Sbjct: 4   KSSDSSHKQSPPSFRKSVEIKATHDQFFVWREFLWGGIAGAFGEGMMHPVDTLKTRLQSQ 63

Query: 73  -LGSCPIKSVGVRQALRSILKTEGPAGFYRGIGAMGLGAGPAHAVYFTVYEDCKKFFSGG 132
            + +   +   + Q LR++   +G  GFYRGI     G+    A YF   E  KK+    
Sbjct: 64  IIMNATQRQKSILQMLRTVWVGDGLKGFYRGIAPGVTGSLATGATYFGFIESTKKWIEES 123

Query: 133 DPN--NSVAHAASGVCATVASDAVFTPMDMVKQRLQLSNSP------------------- 192
            P+     AH  +G         ++ P +++KQR+Q+  +                    
Sbjct: 124 HPSLAGHWAHFIAGAVGDTLGSFIYVPCEVIKQRMQIQGTSSSWSSYISRNSVPVQPRGD 183

Query: 193 ----YKGVLDCVTRVLRDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAK----RGLLEV 252
               Y G+      + +++G K  YA Y +T+  + PF  +    YE  K    +G  + 
Sbjct: 184 MYGYYTGMFQAGCSIWKEQGPKGLYAGYWSTLARDVPFAGLMVVFYEGLKDLTDQGKKKF 243

Query: 253 SPEIVNNEQWIVHATAGAAAGASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIEDVVRT 312
               VN+   I     G  AG  +A++TTPLDVVKT+LQ QG       K     D V  
Sbjct: 244 PQYGVNSS--IEGLVLGGLAGGLSAYLTTPLDVVKTRLQVQG----STIKYKGWLDAVGQ 303

Query: 313 ILKKDGYRGLMRGWVPRMLFHAPAAAICWSTYEAIKSLFQELNGSSDEM 330
           I +K+G +G  RG VPR++++ PA+A+ +   E ++  F+E + +++ +
Sbjct: 304 IWRKEGPQGFFRGSVPRVMWYLPASALTFMAVEFLRDNFREKSNNNNNV 346

BLAST of Csor.00g103010 vs. TAIR 10
Match: AT4G11440.1 (Mitochondrial substrate carrier family protein )

HSP 1 Score: 143.7 bits (361), Expect = 2.7e-34
Identity = 91/277 (32.85%), Postives = 149/277 (53.79%), Query Frame = 0

Query: 46  AGSIAGSVEHMAMFPVDTVKTHMQALGSCPIKSVGVRQALRSILKTEGPAGFYRGIGAMG 105
           AG++AG    + + P+DTVKT +Q   SC ++   +    RSI+   G +G YRGI +  
Sbjct: 332 AGALAGISVSLCLHPLDTVKTMIQ---SCRLEEKSLCNTGRSIISERGFSGLYRGIASNI 391

Query: 106 LGAGPAHAVYFTVYEDCKKFFSGGDPNN--SVAHAASGVCATVASDAVFTPMDMVKQRLQ 165
             + P  A+Y   YE  K       P    S+AH  +G  A++A+  +FTP + +KQ++Q
Sbjct: 392 ASSAPISALYTFTYETVKGTLLPLFPKEYCSLAHCLAGGSASIATSFIFTPSERIKQQMQ 451

Query: 166 LSNSPYKGVLDCVTRVLRDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLLEVSPE 225
           +S S Y+     +  +++  G  + YA +   +  N P + + F  YE  K+ +L     
Sbjct: 452 VS-SHYRNCWTALVGIIQKGGLLSLYAGWTAVLCRNIPHSIIKFYVYENMKQMVLPSPGP 511

Query: 226 IVNNEQ--WIVHATAGAAAGASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIEDVVRTI 285
                Q   +   T G  AG++AAF TTP DVVKT+LQ Q + G  R +  S+   +++I
Sbjct: 512 CGEMAQPTTLQTLTCGGLAGSAAAFFTTPFDVVKTRLQTQ-IPG-SRNQHPSVYQTLQSI 571

Query: 286 LKKDGYRGLMRGWVPRMLFHAPAAAICWSTYEAIKSL 319
            +++G RGL RG +PR++ +    AI +++YE  KS+
Sbjct: 572 RRQEGLRGLYRGLIPRLVMYMSQGAIFFASYEFYKSV 602

BLAST of Csor.00g103010 vs. TAIR 10
Match: AT1G34065.1 (S-adenosylmethionine carrier 2 )

HSP 1 Score: 139.8 bits (351), Expect = 3.9e-33
Identity = 92/288 (31.94%), Postives = 145/288 (50.35%), Query Frame = 0

Query: 41  WQFMIAGSIAGSVEHMAMFPVDTVKTHMQALGSCPIKSVGVRQALRSILKTEGPAGFYRG 100
           ++ +I G +AG V   A++P+DT+KT +Q            R   + I K     G Y G
Sbjct: 79  YESLITGGLAGVVVEAALYPIDTIKTRIQV----------ARDGGKIIWK-----GLYSG 138

Query: 101 IGAMGLGAGPAHAVYFTVYEDCKKFFSGGDPNN--SVAHAASGVCATVASDAVFTPMDMV 160
           +G   +G  PA A++F VYE  K+      P+N  +VAH A+G      S  V  P ++V
Sbjct: 139 LGGNLVGVLPASALFFGVYEPTKQKLLKVLPDNLSAVAHLAAGALGGAVSSIVRVPTEVV 198

Query: 161 KQRLQLSNSPYKGVLDCVTRVLRDEGFKAFYASYRTTVLMNAPFTAVHFATYEAAKRGLL 220
           KQR+Q     +    D V  ++  EGF   YA Y + +L + PF A+ F  YE  + G  
Sbjct: 199 KQRMQ--TGQFVSAPDAVRLIIAKEGFGGMYAGYGSFLLRDLPFDALQFCVYEQLRIGYK 258

Query: 221 EVSPEIVNNEQWIVHATAGAAAGASAAFVTTPLDVVKTQLQCQGVCGCDRFKSGSIEDVV 280
             +   +N+ +   +A  GA AGA    +TTPLDV+KT+L  QG     ++K   + D +
Sbjct: 259 LAARRDLNDPE---NAMIGAFAGAVTGVLTTPLDVIKTRLMVQG--SGTQYK--GVSDCI 318

Query: 281 RTILKKDGYRGLMRGWVPRMLFHAPAAAICWSTYEAIKSLFQELNGSS 327
           +TI++++G   L +G  PR+L+     +I +   E  K +  E +  S
Sbjct: 319 KTIIREEGSSALWKGMGPRVLWIGIGGSIFFGVLEKTKQILSERSQKS 342

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q55DY87.9e-5539.46Mitoferrin OS=Dictyostelium discoideum OX=44689 GN=mcfF PE=3 SV=1[more]
Q620A62.3e-5442.37Mitoferrin OS=Caenorhabditis briggsae OX=6238 GN=mfn-1 PE=3 SV=1[more]
Q9VAY35.1e-5444.32Mitoferrin OS=Drosophila melanogaster OX=7227 GN=mfrn PE=2 SV=1[more]
Q231255.6e-5341.55Mitoferrin OS=Caenorhabditis elegans OX=6239 GN=mfn-1 PE=2 SV=1[more]
Q287T71.8e-5140.98Mitoferrin-1 OS=Danio rerio OX=7955 GN=slc25a37 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022943642.19.47e-242100.00mitoferrin-like [Cucurbita moschata] >KAG6570636.1 Mitoferrin, partial [Cucurbit... [more]
XP_023512983.12.71e-24199.70mitoferrin isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022986893.17.78e-24199.39mitoferrin [Cucurbita maxima][more]
XP_022148452.16.76e-22691.52mitoferrin [Momordica charantia][more]
XP_004143170.18.18e-22592.02mitoferrin [Cucumis sativus] >KGN47047.1 hypothetical protein Csa_020661 [Cucumi... [more]
Match NameE-valueIdentityDescription
A0A6J1FTL24.58e-242100.00mitoferrin-like OS=Cucurbita moschata OX=3662 GN=LOC111448348 PE=3 SV=1[more]
A0A6J1JHV33.77e-24199.39mitoferrin OS=Cucurbita maxima OX=3661 GN=LOC111484495 PE=3 SV=1[more]
A0A6J1D5373.27e-22691.52mitoferrin OS=Momordica charantia OX=3673 GN=LOC111017091 PE=3 SV=1[more]
A0A0A0KF813.96e-22592.02Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G181520 PE=3 SV=1[more]
A0A1S3CK524.71e-22591.77mitoferrin-like OS=Cucumis melo OX=3656 GN=LOC103501881 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G30160.12.1e-14374.39Mitochondrial substrate carrier family protein [more]
AT1G07030.17.4e-14174.39Mitochondrial substrate carrier family protein [more]
AT1G74240.12.4e-3528.94Mitochondrial substrate carrier family protein [more]
AT4G11440.12.7e-3432.85Mitochondrial substrate carrier family protein [more]
AT1G34065.13.9e-3331.94S-adenosylmethionine carrier 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002067Mitochondrial carrier proteinPRINTSPR00926MITOCARRIERcoord: 147..165
score: 25.97
coord: 43..56
score: 22.48
coord: 56..70
score: 32.24
coord: 189..207
score: 18.38
coord: 237..259
score: 24.52
IPR023395Mitochondrial carrier domain superfamilyGENE3D1.50.40.10Mitochondrial carrier domaincoord: 25..166
e-value: 1.9E-34
score: 120.9
IPR023395Mitochondrial carrier domain superfamilyGENE3D1.50.40.10Mitochondrial carrier domaincoord: 167..330
e-value: 3.8E-43
score: 149.4
IPR023395Mitochondrial carrier domain superfamilySUPERFAMILY103506Mitochondrial carriercoord: 36..314
IPR018108Mitochondrial substrate/solute carrierPFAMPF00153Mito_carrcoord: 133..218
e-value: 2.4E-19
score: 69.1
coord: 38..127
e-value: 2.3E-21
score: 75.5
coord: 229..321
e-value: 2.7E-23
score: 81.7
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 228..318
score: 23.328228
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 38..125
score: 21.311638
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 132..216
score: 24.829945
NoneNo IPR availablePANTHERPTHR45758:SF11MITOCHONDRIAL CARRIER PROTEIN, EXPRESSEDcoord: 5..326
NoneNo IPR availablePANTHERPTHR45758MITOFERRIN-1-RELATEDcoord: 5..326

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g103010.m01Csor.00g103010.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0048250 iron import into the mitochondrion
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005381 iron ion transmembrane transporter activity