Csor.00g088220 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g088220
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionPMEI domain-containing protein
LocationCsor_Chr01: 10221201 .. 10221710 (-)
RNA-Seq ExpressionCsor.00g088220
SyntenyCsor.00g088220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSsinglepolypeptidestart_codonstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTTCCATTATTTCTCCTCGCCCTCTTCGCCCTCTTCTCTCCGGCGAAACCAGACGCCGCCCTCATCCGAAACCTCTGCGGCAGAACCGAGCAGCCGGTGATTTGTTCTGCTTGCCTCAATTCCGACTCCGCCAGCCGGACAGCCAATGGGCGTGGACTCGCAGTCATCGCCGTCGGATGTGCGGAGAGGAATACCAGAGCAATGGCCCAGAGGATCGGCGACATTTCCCGTAGAACGCCGAATTCCCCTGTGAAGACGCTGTTGAACGATTGCTGGTTTTCTACAGGGTATGCCGCCGGCAATTTTCCAGGAATCAGAAGTGCGGTCCAAGGAGGAGATTATGCCCGTGCCAGAAACGGCGTGGAGATTTCGATTAGGAATGTCAATAGCTGCTTGGCTGGGTTCGATAAAAACCCTTCGGTCCCGGTGCCGTCGGAGGTTTTGGCGGGGACGGTGGCGGCCGTTCAGAGCTGCCGGATTGTTTTGGGGATATTAAACAACATTTAA

mRNA sequence

ATGCTTCCATTATTTCTCCTCGCCCTCTTCGCCCTCTTCTCTCCGGCGAAACCAGACGCCGCCCTCATCCGAAACCTCTGCGGCAGAACCGAGCAGCCGGTGATTTGTTCTGCTTGCCTCAATTCCGACTCCGCCAGCCGGACAGCCAATGGGCGTGGACTCGCAGTCATCGCCGTCGGATGTGCGGAGAGGAATACCAGAGCAATGGCCCAGAGGATCGGCGACATTTCCCGTAGAACGCCGAATTCCCCTGTGAAGACGCTGTTGAACGATTGCTGGTTTTCTACAGGGTATGCCGCCGGCAATTTTCCAGGAATCAGAAGTGCGGTCCAAGGAGGAGATTATGCCCGTGCCAGAAACGGCGTGGAGATTTCGATTAGGAATGTCAATAGCTGCTTGGCTGGGTTCGATAAAAACCCTTCGGTCCCGGTGCCGTCGGAGGTTTTGGCGGGGACGGTGGCGGCCGTTCAGAGCTGCCGGATTGTTTTGGGGATATTAAACAACATTTAA

Coding sequence (CDS)

ATGCTTCCATTATTTCTCCTCGCCCTCTTCGCCCTCTTCTCTCCGGCGAAACCAGACGCCGCCCTCATCCGAAACCTCTGCGGCAGAACCGAGCAGCCGGTGATTTGTTCTGCTTGCCTCAATTCCGACTCCGCCAGCCGGACAGCCAATGGGCGTGGACTCGCAGTCATCGCCGTCGGATGTGCGGAGAGGAATACCAGAGCAATGGCCCAGAGGATCGGCGACATTTCCCGTAGAACGCCGAATTCCCCTGTGAAGACGCTGTTGAACGATTGCTGGTTTTCTACAGGGTATGCCGCCGGCAATTTTCCAGGAATCAGAAGTGCGGTCCAAGGAGGAGATTATGCCCGTGCCAGAAACGGCGTGGAGATTTCGATTAGGAATGTCAATAGCTGCTTGGCTGGGTTCGATAAAAACCCTTCGGTCCCGGTGCCGTCGGAGGTTTTGGCGGGGACGGTGGCGGCCGTTCAGAGCTGCCGGATTGTTTTGGGGATATTAAACAACATTTAA

Protein sequence

MLPLFLLALFALFSPAKPDAALIRNLCGRTEQPVICSACLNSDSASRTANGRGLAVIAVGCAERNTRAMAQRIGDISRRTPNSPVKTLLNDCWFSTGYAAGNFPGIRSAVQGGDYARARNGVEISIRNVNSCLAGFDKNPSVPVPSEVLAGTVAAVQSCRIVLGILNNI
Homology
BLAST of Csor.00g088220 vs. NCBI nr
Match: KAG6607897.1 (hypothetical protein SDJN03_01239, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 330 bits (845), Expect = 9.81e-114
Identity = 169/169 (100.00%), Postives = 169/169 (100.00%), Query Frame = 0

Query: 1   MLPLFLLALFALFSPAKPDAALIRNLCGRTEQPVICSACLNSDSASRTANGRGLAVIAVG 60
           MLPLFLLALFALFSPAKPDAALIRNLCGRTEQPVICSACLNSDSASRTANGRGLAVIAVG
Sbjct: 1   MLPLFLLALFALFSPAKPDAALIRNLCGRTEQPVICSACLNSDSASRTANGRGLAVIAVG 60

Query: 61  CAERNTRAMAQRIGDISRRTPNSPVKTLLNDCWFSTGYAAGNFPGIRSAVQGGDYARARN 120
           CAERNTRAMAQRIGDISRRTPNSPVKTLLNDCWFSTGYAAGNFPGIRSAVQGGDYARARN
Sbjct: 61  CAERNTRAMAQRIGDISRRTPNSPVKTLLNDCWFSTGYAAGNFPGIRSAVQGGDYARARN 120

Query: 121 GVEISIRNVNSCLAGFDKNPSVPVPSEVLAGTVAAVQSCRIVLGILNNI 169
           GVEISIRNVNSCLAGFDKNPSVPVPSEVLAGTVAAVQSCRIVLGILNNI
Sbjct: 121 GVEISIRNVNSCLAGFDKNPSVPVPSEVLAGTVAAVQSCRIVLGILNNI 169

BLAST of Csor.00g088220 vs. NCBI nr
Match: XP_022940751.1 (uncharacterized protein LOC111446254 [Cucurbita moschata] >KAG7037425.1 hypothetical protein SDJN02_01051, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 330 bits (845), Expect = 1.21e-113
Identity = 169/169 (100.00%), Postives = 169/169 (100.00%), Query Frame = 0

Query: 1   MLPLFLLALFALFSPAKPDAALIRNLCGRTEQPVICSACLNSDSASRTANGRGLAVIAVG 60
           MLPLFLLALFALFSPAKPDAALIRNLCGRTEQPVICSACLNSDSASRTANGRGLAVIAVG
Sbjct: 7   MLPLFLLALFALFSPAKPDAALIRNLCGRTEQPVICSACLNSDSASRTANGRGLAVIAVG 66

Query: 61  CAERNTRAMAQRIGDISRRTPNSPVKTLLNDCWFSTGYAAGNFPGIRSAVQGGDYARARN 120
           CAERNTRAMAQRIGDISRRTPNSPVKTLLNDCWFSTGYAAGNFPGIRSAVQGGDYARARN
Sbjct: 67  CAERNTRAMAQRIGDISRRTPNSPVKTLLNDCWFSTGYAAGNFPGIRSAVQGGDYARARN 126

Query: 121 GVEISIRNVNSCLAGFDKNPSVPVPSEVLAGTVAAVQSCRIVLGILNNI 169
           GVEISIRNVNSCLAGFDKNPSVPVPSEVLAGTVAAVQSCRIVLGILNNI
Sbjct: 127 GVEISIRNVNSCLAGFDKNPSVPVPSEVLAGTVAAVQSCRIVLGILNNI 175

BLAST of Csor.00g088220 vs. NCBI nr
Match: XP_023523451.1 (uncharacterized protein LOC111787665 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 327 bits (837), Expect = 4.98e-112
Identity = 167/169 (98.82%), Postives = 169/169 (100.00%), Query Frame = 0

Query: 1   MLPLFLLALFALFSPAKPDAALIRNLCGRTEQPVICSACLNSDSASRTANGRGLAVIAVG 60
           MLPLFLLALFALFSPAKPDAALIRNLCGRTEQPVICSACLNSDSASRTANGRGLAVIAVG
Sbjct: 33  MLPLFLLALFALFSPAKPDAALIRNLCGRTEQPVICSACLNSDSASRTANGRGLAVIAVG 92

Query: 61  CAERNTRAMAQRIGDISRRTPNSPVKTLLNDCWFSTGYAAGNFPGIRSAVQGGDYARARN 120
           CAERNTRAMAQRIGDISRRTPNSPVKTLLNDCWFSTGYAAGNFPGIR+AVQGGDYARARN
Sbjct: 93  CAERNTRAMAQRIGDISRRTPNSPVKTLLNDCWFSTGYAAGNFPGIRNAVQGGDYARARN 152

Query: 121 GVEISIRNVNSCLAGFDKNPSVPVPSEVLAGTVAAVQSCRIVLGILNNI 169
           GVEISIRNVNSCLAGFDKNPSVPVPSEVLAGTVAAVQSCRIVLGILN+I
Sbjct: 153 GVEISIRNVNSCLAGFDKNPSVPVPSEVLAGTVAAVQSCRIVLGILNHI 201

BLAST of Csor.00g088220 vs. NCBI nr
Match: XP_022981703.1 (uncharacterized protein LOC111480766 [Cucurbita maxima])

HSP 1 Score: 320 bits (821), Expect = 5.52e-110
Identity = 164/169 (97.04%), Postives = 167/169 (98.82%), Query Frame = 0

Query: 1   MLPLFLLALFALFSPAKPDAALIRNLCGRTEQPVICSACLNSDSASRTANGRGLAVIAVG 60
           MLPLFLL++FA FSPAKPDAALIRNLCGRTEQPVICSACLNSDSASRTANGRGLAVIAVG
Sbjct: 7   MLPLFLLSVFAPFSPAKPDAALIRNLCGRTEQPVICSACLNSDSASRTANGRGLAVIAVG 66

Query: 61  CAERNTRAMAQRIGDISRRTPNSPVKTLLNDCWFSTGYAAGNFPGIRSAVQGGDYARARN 120
           CAERNTRAMAQRIGDISRRTPNSPVKTLLNDCWFSTGYAAGNFPGIRSAV+ GDYARARN
Sbjct: 67  CAERNTRAMAQRIGDISRRTPNSPVKTLLNDCWFSTGYAAGNFPGIRSAVEEGDYARARN 126

Query: 121 GVEISIRNVNSCLAGFDKNPSVPVPSEVLAGTVAAVQSCRIVLGILNNI 169
           GVEISIRNVNSCLAGFDKNPSVPVPSEVLAGTVAAVQSCRIVLGILNNI
Sbjct: 127 GVEISIRNVNSCLAGFDKNPSVPVPSEVLAGTVAAVQSCRIVLGILNNI 175

BLAST of Csor.00g088220 vs. NCBI nr
Match: XP_038896712.1 (pectinesterase inhibitor-like [Benincasa hispida])

HSP 1 Score: 232 bits (592), Expect = 4.02e-75
Identity = 117/164 (71.34%), Postives = 135/164 (82.32%), Query Frame = 0

Query: 6   LLALFALFSPAKPDAALIRNLCGRTEQPVICSACLNSDSASRTANGRGLAVIAVGCAERN 65
           ++ LFALFSPAKPDAALI NLC +TEQP ICS CLNS S S+TA+G GLA+IA+GCAE+N
Sbjct: 10  IIVLFALFSPAKPDAALIANLCCKTEQPKICSDCLNSGSGSQTADGLGLALIAIGCAEKN 69

Query: 66  TRAMAQRIGDISRRTPNSPVKTLLNDCWFSTGYAAGNFPGIRSAVQGGDYARARNGVEIS 125
           T+ MAQR+G + R TP+S +KTLLNDCW STG AAGNFPGI  +V  GDYA  RN +EI+
Sbjct: 70  TKLMAQRLGGLVRSTPDSTLKTLLNDCWLSTGSAAGNFPGIARSVAAGDYAGGRNVLEIT 129

Query: 126 IRNVNSCLAGFDKNPSVPVPSEVLAGTVAAVQSCRIVLGILNNI 169
           IRN NSCL  F+KNPSVPVPSEVLAGTVAA Q CRI+  ILNNI
Sbjct: 130 IRNANSCLDNFNKNPSVPVPSEVLAGTVAAAQDCRIISEILNNI 173

BLAST of Csor.00g088220 vs. ExPASy TrEMBL
Match: A0A6J1FJC0 (uncharacterized protein LOC111446254 OS=Cucurbita moschata OX=3662 GN=LOC111446254 PE=4 SV=1)

HSP 1 Score: 330 bits (845), Expect = 5.86e-114
Identity = 169/169 (100.00%), Postives = 169/169 (100.00%), Query Frame = 0

Query: 1   MLPLFLLALFALFSPAKPDAALIRNLCGRTEQPVICSACLNSDSASRTANGRGLAVIAVG 60
           MLPLFLLALFALFSPAKPDAALIRNLCGRTEQPVICSACLNSDSASRTANGRGLAVIAVG
Sbjct: 7   MLPLFLLALFALFSPAKPDAALIRNLCGRTEQPVICSACLNSDSASRTANGRGLAVIAVG 66

Query: 61  CAERNTRAMAQRIGDISRRTPNSPVKTLLNDCWFSTGYAAGNFPGIRSAVQGGDYARARN 120
           CAERNTRAMAQRIGDISRRTPNSPVKTLLNDCWFSTGYAAGNFPGIRSAVQGGDYARARN
Sbjct: 67  CAERNTRAMAQRIGDISRRTPNSPVKTLLNDCWFSTGYAAGNFPGIRSAVQGGDYARARN 126

Query: 121 GVEISIRNVNSCLAGFDKNPSVPVPSEVLAGTVAAVQSCRIVLGILNNI 169
           GVEISIRNVNSCLAGFDKNPSVPVPSEVLAGTVAAVQSCRIVLGILNNI
Sbjct: 127 GVEISIRNVNSCLAGFDKNPSVPVPSEVLAGTVAAVQSCRIVLGILNNI 175

BLAST of Csor.00g088220 vs. ExPASy TrEMBL
Match: A0A6J1J2U5 (uncharacterized protein LOC111480766 OS=Cucurbita maxima OX=3661 GN=LOC111480766 PE=4 SV=1)

HSP 1 Score: 320 bits (821), Expect = 2.67e-110
Identity = 164/169 (97.04%), Postives = 167/169 (98.82%), Query Frame = 0

Query: 1   MLPLFLLALFALFSPAKPDAALIRNLCGRTEQPVICSACLNSDSASRTANGRGLAVIAVG 60
           MLPLFLL++FA FSPAKPDAALIRNLCGRTEQPVICSACLNSDSASRTANGRGLAVIAVG
Sbjct: 7   MLPLFLLSVFAPFSPAKPDAALIRNLCGRTEQPVICSACLNSDSASRTANGRGLAVIAVG 66

Query: 61  CAERNTRAMAQRIGDISRRTPNSPVKTLLNDCWFSTGYAAGNFPGIRSAVQGGDYARARN 120
           CAERNTRAMAQRIGDISRRTPNSPVKTLLNDCWFSTGYAAGNFPGIRSAV+ GDYARARN
Sbjct: 67  CAERNTRAMAQRIGDISRRTPNSPVKTLLNDCWFSTGYAAGNFPGIRSAVEEGDYARARN 126

Query: 121 GVEISIRNVNSCLAGFDKNPSVPVPSEVLAGTVAAVQSCRIVLGILNNI 169
           GVEISIRNVNSCLAGFDKNPSVPVPSEVLAGTVAAVQSCRIVLGILNNI
Sbjct: 127 GVEISIRNVNSCLAGFDKNPSVPVPSEVLAGTVAAVQSCRIVLGILNNI 175

BLAST of Csor.00g088220 vs. ExPASy TrEMBL
Match: A0A5D3DXI0 (Pectinesterase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G001540 PE=4 SV=1)

HSP 1 Score: 228 bits (581), Expect = 9.18e-74
Identity = 113/161 (70.19%), Postives = 130/161 (80.75%), Query Frame = 0

Query: 9   LFALFSPAKPDAALIRNLCGRTEQPVICSACLNSDSASRTANGRGLAVIAVGCAERNTRA 68
           LFALFSPAKPDAA I NLC +TEQP ICS CLNS   S++A+GRGLA+IAVGCAERNT+ 
Sbjct: 13  LFALFSPAKPDAAYIGNLCSKTEQPKICSDCLNSGRGSQSASGRGLALIAVGCAERNTKL 72

Query: 69  MAQRIGDISRRTPNSPVKTLLNDCWFSTGYAAGNFPGIRSAVQGGDYARARNGVEISIRN 128
           MAQR+G++ R TP S +K LLN+CW STGYAAGNFPGI  +V  GDYA  +N +EI+IRN
Sbjct: 73  MAQRVGNLVRSTPESTLKMLLNECWMSTGYAAGNFPGIARSVAAGDYASGKNVLEITIRN 132

Query: 129 VNSCLAGFDKNPSVPVPSEVLAGTVAAVQSCRIVLGILNNI 169
           VN CL  F KNP++PVPSEVLAGTVA  Q CRIV  ILNNI
Sbjct: 133 VNMCLVNFKKNPNIPVPSEVLAGTVAVTQDCRIVTQILNNI 173

BLAST of Csor.00g088220 vs. ExPASy TrEMBL
Match: A0A1S3CK48 (pectinesterase inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103501854 PE=4 SV=1)

HSP 1 Score: 228 bits (581), Expect = 9.18e-74
Identity = 113/161 (70.19%), Postives = 130/161 (80.75%), Query Frame = 0

Query: 9   LFALFSPAKPDAALIRNLCGRTEQPVICSACLNSDSASRTANGRGLAVIAVGCAERNTRA 68
           LFALFSPAKPDAA I NLC +TEQP ICS CLNS   S++A+GRGLA+IAVGCAERNT+ 
Sbjct: 13  LFALFSPAKPDAAYIGNLCSKTEQPKICSDCLNSGRGSQSASGRGLALIAVGCAERNTKL 72

Query: 69  MAQRIGDISRRTPNSPVKTLLNDCWFSTGYAAGNFPGIRSAVQGGDYARARNGVEISIRN 128
           MAQR+G++ R TP S +K LLN+CW STGYAAGNFPGI  +V  GDYA  +N +EI+IRN
Sbjct: 73  MAQRVGNLVRSTPESTLKMLLNECWMSTGYAAGNFPGIARSVAAGDYASGKNVLEITIRN 132

Query: 129 VNSCLAGFDKNPSVPVPSEVLAGTVAAVQSCRIVLGILNNI 169
           VN CL  F KNP++PVPSEVLAGTVA  Q CRIV  ILNNI
Sbjct: 133 VNMCLVNFKKNPNIPVPSEVLAGTVAVTQDCRIVTQILNNI 173

BLAST of Csor.00g088220 vs. ExPASy TrEMBL
Match: A0A0A0L044 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G628950 PE=4 SV=1)

HSP 1 Score: 221 bits (564), Expect = 3.55e-71
Identity = 111/161 (68.94%), Postives = 129/161 (80.12%), Query Frame = 0

Query: 9   LFALFSPAKPDAALIRNLCGRTEQPVICSACLNSDSASRTANGRGLAVIAVGCAERNTRA 68
           LFALFSPAKPDAA I+NLCG+TEQP ICS CLNS   S++A GRGLA+IAV CAERNT+ 
Sbjct: 13  LFALFSPAKPDAAYIQNLCGKTEQPKICSDCLNSGQGSQSAIGRGLALIAVECAERNTKL 72

Query: 69  MAQRIGDISRRTPNSPVKTLLNDCWFSTGYAAGNFPGIRSAVQGGDYARARNGVEISIRN 128
           MAQR+G++ R TP S +KTLLN+CW STGYAAGNFPGI  +V  GDY   ++ +EI+IRN
Sbjct: 73  MAQRVGNLVRSTPESTLKTLLNECWMSTGYAAGNFPGIARSVAAGDYGGGKSVLEITIRN 132

Query: 129 VNSCLAGFDKNPSVPVPSEVLAGTVAAVQSCRIVLGILNNI 169
           VN C+  F KNPS+ VPSEVLAGTVA  Q CRIV  ILNNI
Sbjct: 133 VNMCMDNFKKNPSILVPSEVLAGTVAVTQDCRIVTQILNNI 173

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
KAG6607897.19.81e-114100.00hypothetical protein SDJN03_01239, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022940751.11.21e-113100.00uncharacterized protein LOC111446254 [Cucurbita moschata] >KAG7037425.1 hypothet... [more]
XP_023523451.14.98e-11298.82uncharacterized protein LOC111787665 [Cucurbita pepo subsp. pepo][more]
XP_022981703.15.52e-11097.04uncharacterized protein LOC111480766 [Cucurbita maxima][more]
XP_038896712.14.02e-7571.34pectinesterase inhibitor-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1FJC05.86e-114100.00uncharacterized protein LOC111446254 OS=Cucurbita moschata OX=3662 GN=LOC1114462... [more]
A0A6J1J2U52.67e-11097.04uncharacterized protein LOC111480766 OS=Cucurbita maxima OX=3661 GN=LOC111480766... [more]
A0A5D3DXI09.18e-7470.19Pectinesterase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3CK489.18e-7470.19pectinesterase inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103501854 PE=4 SV=1[more]
A0A0A0L0443.55e-7168.94PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G628950 PE=4 S... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 18..165
e-value: 6.4E-13
score: 58.9
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 22..146
e-value: 5.3E-13
score: 49.5
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 4..168
e-value: 2.0E-22
score: 77.3
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 19..169
e-value: 6.9E-23
score: 83.3
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 21..168
NoneNo IPR availablePANTHERPTHR31080PECTINESTERASE INHIBITOR-LIKEcoord: 2..169
NoneNo IPR availablePANTHERPTHR31080:SF83CELL WALL / VACUOLAR INHIBITOR OF FRUCTOSIDASE 2-LIKEcoord: 2..169
IPR034086Pectinesterase inhibitor, plantCDDcd15797PMEIcoord: 19..169
e-value: 2.25687E-25
score: 93.2557

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g088220.m01Csor.00g088220.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity